Extreme ozone pollution events(EOPEs)are associated with synoptic weather patterns(SWPs)and pose severe health and ecological risks.However,a systematic investigation of themeteorological causes,transport pathways,and...Extreme ozone pollution events(EOPEs)are associated with synoptic weather patterns(SWPs)and pose severe health and ecological risks.However,a systematic investigation of themeteorological causes,transport pathways,and source contributions to historical EOPEs is still lacking.In this paper,the K-means clustering method is applied to identify six dominant SWPs during the warm season in the Yangtze River Delta(YRD)region from 2016 to 2022.It provides an integrated analysis of the meteorological factors affecting ozone pollution in Hefei under different SWPs.Using the WRF-FLEXPART model,the transport pathways(TPPs)and geographical sources of the near-surface air masses in Hefei during EOPEs are investigated.The results reveal that Hefei experienced the highest ozone concentration(134.77±42.82μg/m^(3)),exceedance frequency(46 days(23.23%)),and proportion of EOPEs(21 instances,47.7%)under the control of peripheral subsidence of typhoon(Type 5).Regional southeast winds correlated with the ozone pollution in Hefei.During EOPEs,a high boundary layer height,solar radiation,and temperature;lowhumidity and cloud cover;and pronounced subsidence airflow occurred over Hefei and the broader YRD region.The East-South(E_S)patterns exhibited the highest frequency(28 instances,65.11%).Regarding the TPPs and geographical sources of the near-surface air masses during historical EOPEs.The YRD was the main source for land-originating air masses under E_S patterns(50.28%),with Hefei,southern Anhui,southern Jiangsu,and northern Zhejiang being key contributors.These findings can help improve ozone pollution early warning and control mechanisms at urban and regional scales.展开更多
In the context of urbanization,air pollution has emerged as a significant environmental challenge.A thorough understanding of their transport pathways,especially at a national scale,is essential for environmental prot...In the context of urbanization,air pollution has emerged as a significant environmental challenge.A thorough understanding of their transport pathways,especially at a national scale,is essential for environmental protection and policy-making.However,it remains partially elusive due to the constraints of available data and analytical methods.This study proposed a data-driven spatiotemporal correlation analysis method employing the Dynamic Time Warping(DTW).We represented the first comprehensive attempt to chart the long-term and nationwide transport pathways of PM_(2.5) utilizing an extensive dataset spanning from 2000 to 2021 across China,which is crucial for understanding long-term air pollution trends.Compared with traditional chemical transport models(CTMs),this data-driven method can generate transport pathways of PM_(2.5) without requiring extensive meteorological or emission data,and suggesting fundamentally consistent spatial distribution and trends.Our analysis reveals that China’s transport pathways are notably pronounced in the Northwest(34%of the total pathways in China),Southwest(22%),and North(21%)regions,with less significant pathways in the Northeast(10%)region and isolated occurrences elsewhere.Additionally,a notable decrease in the number of China’s PM_(2.5) transport pathways,similar to annual average concentrations,was observed after 2013,aligning with stricter environmental regulations.Furthermore,we have demonstrated the feasibility of applying our method to the transport pathways of other gaseous pollutants.The approach is effective in detecting and quantifying air pollutants’transport pathways,even in regions like the Northwest with limited monitoring infrastructure,which may aid in environmental decision-making.The study will notably improve the current understanding of air pollutants’transport process,providing a new perspective for studying the large-scale spatiotemporal correlations.展开更多
Interface modification has been demonstrated as an effective means to enhance the performance of perovskite solar cells.However,the effect depends on the anchoring mode and strength of the interfacial molecules,which ...Interface modification has been demonstrated as an effective means to enhance the performance of perovskite solar cells.However,the effect depends on the anchoring mode and strength of the interfacial molecules,which determines whether long-term robust interface for carrier viaduct can be achieved under operational light illumination.Herein,we select squaric acid(SA)as the interfacial molecule between the perovskite and SnO_(2)layer and propose a selfregulated bilateral anchoring strategy.The unique four-membered ring conjugated structure and dicarboxylic acid groups facilitate stable hydrogen bonds and coordination bonds at both SnO_(2)/SA and SA/PbI_(2)interfaces.The self-transforming property of SA enables the dynamic bilateral anchoring at the buried interface,ultimately releasing residual stress and constructing a stable interfacial molecular bridge.The results show that SA molecular bridge not only can effectively inhibit the generation of diverse charged defects but also serves as an effective electron transport pathway,resulting in improved power conversion efficiency(PCE)from 23.19 to 25.50%and excellent stability at the maximum power point.Additionally,the PCEs of the flexible and large-area(1 cm^(2))devices were increased to 24.92%and 24.01%,respectively,demonstrating the universal applicability of the bilateral anchoring to PSCs based on different substrates and larger area.展开更多
In situ X-ray diffraction(XRD)has been widely employed to probe the structural evolution of lithium-ion battery electrodes.However,electrodes with practically relevant high-energy densities often exhibit pronounced el...In situ X-ray diffraction(XRD)has been widely employed to probe the structural evolution of lithium-ion battery electrodes.However,electrodes with practically relevant high-energy densities often exhibit pronounced electrochemical inhomogeneities,which substantially hinder accurate structu ral interpretatio n.In this study,we systematically investigate the origin and characteristics of localized electrochemical dead zones(LEDZs)observed during in situ XRD measurements.Through comprehensive analyses using modified coin cells and pouch cells containing various cathode materials under diverse electrochemical conditions,we identify insufficient electron and ion conductivity as the prima ry cause of LEDZ formation.Crucially,our results demonstrate that decoupling electron and ion transport pathways from the X-ray beam direction-an effect inherently realized in pouch cell configurations-effectively mitigates LEDZs,thereby enabling artifact-free structural analysis.This work underscores the critical role of cell geometry and conductivity pathways in achieving accurate structural characterization,providing fundamental guidelines for the design and interpretation of high-energy-density lithium-ion battery electrodes.展开更多
This study tracked the characteristics of atmospheric wet deposition of the toxic element arsenic(As)at both urban(Guangzhou(GZ))and forested(Dinghushan Natural Reserve(DHS))sites within the Pearl River Delta(PRD)regi...This study tracked the characteristics of atmospheric wet deposition of the toxic element arsenic(As)at both urban(Guangzhou(GZ))and forested(Dinghushan Natural Reserve(DHS))sites within the Pearl River Delta(PRD)region between 2016 and 2019,examining its correlation with rainfall patterns.Additionally,by employing backward trajectory analysis and the potential source contribution function(PSCF)in conjunction with pertinent emission inventories,we pinpointed the main pathways of atmospheric arsenic transport and evaluated the emission contributions from priority source areas.The study revealed that the atmospheric arsenic wet deposition fluxes at the GZ and DHS sites exhibited a trend of increase followed by a decrease over the four-year period.Wet season deposition fluxes were more than triple those of the dry season,with urban site showing a difference of over four times.Notably,wet season As deposition at both sites was predominantly affected by heavy rainfall from marine air masses,constituting 31%of the total deposition.The predominant trajectory directions contributing to arsenic deposition at GZ and DHS were northeast(55%)and south(53%),respectively.The primary source areas for both sites were largely outside the PRD region,with the GZ site having 80%to 95%of its source area in the non-PRD region,compared to 69%to 88%at the DHS site.Furthermore,non-PRD areas contributed approximately 65%to arsenic emissions for both sites,with the industrial sector being the dominant emission source,exceeding 97%of the total emissions.展开更多
This study attempts to identify the dominant transport pathways,potential source areas,and their seasonal variation at sites with high inorganic nitrogen(IN)wet deposition flux in southern China.This is a long-term st...This study attempts to identify the dominant transport pathways,potential source areas,and their seasonal variation at sites with high inorganic nitrogen(IN)wet deposition flux in southern China.This is a long-term study(2010-2017)based on continuous deposition measurements at the Guangzhou urban site(GZ)and the Dinghushan Natural Reserve site(DHS)located in the Pearl River Delta(PRD)region.A dataset on monthly IN concentration in precipitation and wet deposition flux were provided.The average annual fluxes measured at both sites(GZ:33.04±9.52,DHS:20.52±10.22 kg N/(ha·year))were higher,while the ratios of reduced to oxidized N(GZ:1.19±0.77,DHS:1.25±0.84)were lower compared with the national mean level and the previous reported level throughout the PRD region.The dominant pathways were not always consistent with the highest proportional trajectory clusters.The transport pathways contributing most of deposition were identified in the north and northnortheast in the dry season and in the east-southeast,east,and south-southwest in the wet season.A weighted potential source contribution function(WPSCF)value>0.3 was determined reasonably to define the potential source area.Emission within the PRD region contributed the majority(≥95%at both sites)of the IN deposition in the wet season,while the contribution outside the region increased significantly in the dry season(GZ:27.86%,DHS:95.26%).Our results could help create more effective policy to control precursor emissions for IN fluxes,enabling reduction of the ecological risks due to excessive nitrogen.展开更多
Guided by the analysis of source-to-sink system,this study investigates the Paleogene Oligocene Lingshui Formation in the Qiongdongnan Basin by comparing the geological characterizes in land and sea areas and integrat...Guided by the analysis of source-to-sink system,this study investigates the Paleogene Oligocene Lingshui Formation in the Qiongdongnan Basin by comparing the geological characterizes in land and sea areas and integrating outcrop,core,drilling,logging and 3D seismic data,to systematically analyze the characteristics of the source,transport pathway,and sink during the deposition of Lingshui Formation,and reveal the patterns,controlling factors and petroleum geologic significance of the source-to-sink systems.The results are obtained in five aspects.First,during the fault-depression transition,the Qiongdongnan Basin received sediments from the provenances presenting as segments in east-west and zones in north-south,primarily with the Indosinian granites in the Shenhu Uplift in the east and the Yanshanian granites in the west.Overall,the sources are young in the southern and northern parts and old in the interior of the basin.Second,three types of sediment transport pathways are identified:paleo-valleys,fault troughs and trough-valley transitional zones.Third,based on differences in sediment supply modes,the unique source-to-sink systems during the fault-depression transition in marine rift basins are categorized into three types:exogenous,endogenous and composite.Fourth,the characteristics of these source-to-sink systems are primarily controlled by provenance,paleogeomorphology,and sea-level changes.Provenance lithology and scale dictated the composition and volume of sedimentary deposits.Paleogeomorphology influenced erosion intensity in the provenance and the development of paleodrainage systems,thereby affecting the distribution and types of sedimentary systems.Additionally,sea-level changes decided the extent of the provenance,but also regulated the sediment distribution patterns through oceanic processes such as waves and tides.Fifth,the exogenous source-to-sink systems may form large-scale reservoir bodies,the endogenous systems develop secondary pores due to presence of soluble minerals,and the composite systems demonstrate reservoir properties varying from area to area.展开更多
Regarding the rapid shrinkage of the Arctic cryosphere, sea ice plays a significant role in the temporal storage,transport, and release of microplastics(an emergent pollutant) among atmospheric, aquatic, and terrestri...Regarding the rapid shrinkage of the Arctic cryosphere, sea ice plays a significant role in the temporal storage,transport, and release of microplastics(an emergent pollutant) among atmospheric, aquatic, and terrestrial environments. However, there are sparse studies on microplastics in the landfast sea ice and lagoon lake ice in the Alaskan Arctic region. Therefore, this study investigated characteristics and potential sources of microplastics in the landfast sea ice and lagoon lake ice in the Alaskan Arctic(Point Barrow). The results found that the average abundance of microplastics in the landfast sea ice(220.6±140.1 items/L) was comparable to that in lagoon lake ice near Point Barrow(148.9±141.8 items/L). For different layers of sea ice cores, the maximum abundance of microplastics generally occurred in the bottom layer. The overall particle sizes for the detected microplastics revealed that the abundance of microplastics decreased with increasing size for both landfast sea ice and lagoon lake ice samples. Small-sized microplastics(≤50 μm) accounted for more than 80% of the detected microplastics,with the dominant shape being fragments. The predominant polymers in sea ice were polyamide(PA), polyethylene(PE), and polyethylene terephthalate(PET). Meanwhile, PE and rubber dominated the polymers detected in lagoon lake ice. These differences between microplastics in Arctic sea ice and lagoon lake ice further indicated the discrepancies in microplastic transport pathways and deposition. Microplastics in landfast sea ice were mainly affected by seawater transported from the Pacific Ocean into the Chukchi Sea. In contrast, microplastics in lagoon lake ice were mostly influenced by the seawater of the Beaufort Sea and local vehicle emissions(e.g., rubber). This study further highlighted that a large abundance of microplastics was widely distributed in the sea ice of the Alaska Arctic region and may pose potential risks to the local ecosystems.展开更多
Continuous observations of mass concentration and elemental composition of aerosol particles （PM2.5） were conducted at Tongyu, a semi-arid site in Northeast China in the spring of 2006. The average mass concentratio...Continuous observations of mass concentration and elemental composition of aerosol particles （PM2.5） were conducted at Tongyu, a semi-arid site in Northeast China in the spring of 2006. The average mass concentration of PM2.5 at Tongyu station was 260.9±274.4 μg m^-3 during the observation period. Nine dust events were monitored with a mean concentration of 528.0±302.7 μgm^-3. The PM2.5 level during non- dust storm （NDS） period was 111.65±63.37 μg m^-3. High mass concentration shows that fine-size particles pollution was very serious in the semi-arid area in Northeast China. The enrichment factor values for crust elements during the dust storm （DS） period are close to those in the NDS period, while the enrichment factor values for pollution elements during the NDS period are much higher than those in the DS period, showing these elements were from anthropogenic sources. The ratios of dust elements to Fe were relative constant during the DS period. The Ca/Fe ratio in dust aerosols at Tongyu is remarkably different from that observed in other source regions and downwind regions. Meteorological analysis shows that dust events at Tongyu are usually associated with dry, low pressure and high wind speed weather conditions. Air mass back-trajectory analysis identified three kinds of general pathways were associated with the aerosol particle transport to Tongyu, and the northwest direction pathway was the main transport route.展开更多
The objective of this study was to characterize the mass concentration and chemical composition of aerosol particles（PM2.5） collected at Tongliao（Inner Mongolia Autonomous Region, China）, a site in Horqin Sand-lan...The objective of this study was to characterize the mass concentration and chemical composition of aerosol particles（PM2.5） collected at Tongliao（Inner Mongolia Autonomous Region, China）, a site in Horqin Sand-land in northeast China. During spring 2005, the mass concentration for PM2.5 was （126±71）μg/m^3 in average. Five dust storm events were monitored with higher concentration of （255 ± 77）μg/m^3 in average than the non dusty days of （106 ± 44）μg/m^3. Concentrations for 20 elements were obtained by the PIXE method. Mass concentrations of ALl, Mg, Si, K, Ca, Ti, Mn, and V, which increased with the PM2.5 concentration, were higher than the pollution elements （S, Cl, Zn, Ar, Se, Br, and Pb）. Enrichment factor relative to crust material was also calculated, which showed dust trace elements were mainly from earth upper crust and pollution elements were dominated the anthropogenic aerosols. The Si/Al, Ca/Al, and Fe/Al ratios in PM2.5 samples at Tongliao were 4.07, 0.94, and 0.82, respectively, which were remarkably different with those on other source regions, such as ＂Western desert source region＂, ＂North desert source region＂ and central Asia source. Air mass back-trajectory analysis identified three kinds of general pathways were associated with the aerosol particle transport to Tongliao, but have the similar elemental ratios, implying that elemental signatures for dust aerosol from Horqin Sand-land were different with other regions.展开更多
This study presents a Lagrangian view of upper water exchanges across the Luzon Strait based on the finite-time Lyapunov exponents(FTLE)fields computed from the surface geostrophic current.The Lagrangian coherent stru...This study presents a Lagrangian view of upper water exchanges across the Luzon Strait based on the finite-time Lyapunov exponents(FTLE)fields computed from the surface geostrophic current.The Lagrangian coherent structures(LCSs)extracted from the FTLE fields well identify the typical flow patterns and eddy activities around the Luzon Strait.In addition,they reveal the intricate transport paths and fluid domains,which are validated by the tracks of satellite-tracked surface drifters and cannot be visually recognized in the velocity maps.The FTLE fields indicate that there are mainly four types of transport patterns near the Luzon Strait;among them,the Kuroshio northward-flowing"leaping"pattern and the clockwise rotating"looping"pattern occur more frequently than the"leaking"pattern of the direct Kuroshio branch into the SCS and the"outflowing"pattern from the SCS to the Pacific.The eddy shedding events of the Kuroshio at the Luzon Strait are further analyzed,and the importance of considering LCSs in estimating transport by eddies is highlighted.The anticyclonic eddy(ACE)shedding cases reveal that ACEs mainly originate from the looping paths of Kuroshio and thus could effectively trap the Kuroshio water before eddy detachments.LCSs provide useful information to predict the positions of the upstream waters that finally enter the ACEs.In contrast,LCS snapshots indicate that during the formation of cyclonic eddies(CEs),most CEs are not connected with the pathways of Kuroshio water.Hence,the contribution of CEs to the surface water exchanges from the Pacific into the SCS is tiny.展开更多
Baicalin is a flavonoid compound extracted from Scutellaria baicalensis root.Recent evidence indicates that baicalin is neuroprotective in models of ischemic stroke.Here,we investigate the neuroprotective effect of ba...Baicalin is a flavonoid compound extracted from Scutellaria baicalensis root.Recent evidence indicates that baicalin is neuroprotective in models of ischemic stroke.Here,we investigate the neuroprotective effect of baicalin in a neonatal rat model of hypoxic-ischemic encephalopathy.Seven-day-old pups underwent left common carotid artery ligation followed by hypoxia（8% oxygen at 37°C） for 2 hours,before being injected with baicalin（120 mg/kg intraperitoneally） and examined 24 hours later.Baicalin effectively reduced cerebral infarct volume and neuronal loss,inhibited apoptosis,and upregulated the expression of p-Akt and glutamate transporter 1.Intracerebroventricular injection of the phosphoinositide 3-kinase/protein kinase B（PI3 K/Akt） inhibitor LY294002 30 minutes before injury blocked the effect of baicalin on p-Akt and glutamate transporter 1,and weakened the associated neuroprotective effect.Our findings provide the first evidence,to our knowledge that baicalin can protect neonatal rat brains against hypoxic-ischemic injury by upregulating glutamate transporter 1 via the PI3 K/Akt signaling pathway.展开更多
Objective To investigate the potential involvement of DMT1（IRE） protein in the brain vascular system in vivo during Pb exposure. Methods Three groups of male Sprague-Dawley rats were exposed to Pb in drinking water,...Objective To investigate the potential involvement of DMT1（IRE） protein in the brain vascular system in vivo during Pb exposure. Methods Three groups of male Sprague-Dawley rats were exposed to Pb in drinking water, among which two groups were concurrently administered by oral gavage once every other day as the low and high Fe treatment group, respectively, for 6 weeks. At the same time, the group only supplied with high Fe was also set as a reference. The animals were decapitated, then brain capillary-rich fraction was isolate from cerebral cortex. Western blot method was used to identify protein expression, and RT-PCR to detect the change of the m RNA. Results Pb exposure significantly increased Pb concentrations in cerebral cortex. Low Fe dose significantly reduced the cortex Pb levels, However, high Fe dose increased the cortex Pb levels. Interestingly, changes of DMT1（IRE） protein in brain capillary-rich fraction were highly related to the Pb level, but those of DMT1（IRE） m RNA were not significantly different. Moreover, the consistent changes in the levels of p-ERK1/2 or IRP1 with the changes in the levels of DMT1（IRE）. Conclusion These results suggest that Pb is transported into the brain through DMT1（IRE）, and the ERK MAPK pathway is involved in DMT1（IRE）-mediated transport regulation in brain vascular system in vivo.展开更多
The confined ionic liquid(IL) in solid polymer composite electrolytes(SCPEs) can improve the performance of lithium metal batteries. However, the impact/role and working mechanism of confined IL in SCPEs remain ambigu...The confined ionic liquid(IL) in solid polymer composite electrolytes(SCPEs) can improve the performance of lithium metal batteries. However, the impact/role and working mechanism of confined IL in SCPEs remain ambiguous. Herein, IL was immobilized on SiO_(2)(SiO_(2)@IL-C) and then used to prepare the confined SCPEs together with LiTFSI and PEO to study the impacts of confined-IL on the properties and performance of electrolytes and reveal the Li+transport mechanism. The results show that, compared to the IL-unconfined SCPE, the IL-confined ones exhibit better performance of electrolytes and cells, such as higher ionic conductivity, higher t+Li, and wider electrochemical windows, as well as more stable cycle performance, due to the increased dissociation degree of lithium salt and enlarged polymer amorphousness. The finite-element/molecular-dynamics simulations suggest that the IL confined on the SiO_(2) provided an additional Li+transport pathway(Li+→ SiO_(2)@IL-C) that can accelerate ion transfer and alleviate lithium dendrites, leading to ultrastable stripping/plating cycling over 1900 h for the Li/SCPEs/Li symmetric cells. This study demonstrates that IL-confinement is an effective strategy for the intelligent approach of high-performance lithium metal batteries.展开更多
Intraocular injection of anti-vascular endothelial growth factor(VEGF)antibodies is the first-line treatment for ocular neovascular diseases.However,the invasive nature of this administration method often reduces pati...Intraocular injection of anti-vascular endothelial growth factor(VEGF)antibodies is the first-line treatment for ocular neovascular diseases.However,the invasive nature of this administration method often reduces patient compliance and negatively affects treatment outcomes.Noninvasive formulations of anti-VEGF antibody are urgently needed,but their development remains challenging due to the complex ocular barriers.This study identified an anti-VEGF singledomain antibody(sdVE01)that is three times smaller than the commercially available ranibizumab,yet retains a comparable anti-angiogenic effect to the heavy-chain region of ranibizumab(VHHL).Additionally,four dithiolane molecules(DM)were designed to construct DM-based antibody nanoformulations,which effectively penetrate both the anterior and posterior segments of the eye.Upon eyedrop administration,DM-based antibody nanoformulations significantly inhibited the VEGF pathway and reduced neovascularization in a corneal alkali-burn rat model.Notably,the therapeutic effects of the antibody eyedrops were comparable to those of ranibizumab administered via subconjunctival injection.Overall,the dithiolane-based antibody eyedrops represent a promising noninvasive strategy for treating ocular neovascularization diseases.展开更多
The proton conductive electrolytes operating under high temperature(100-200℃)are highly desired for high temperature proton exchange membrane fuel cells(HTPEMFC),which possess high CO tolerance,easy thermal-water man...The proton conductive electrolytes operating under high temperature(100-200℃)are highly desired for high temperature proton exchange membrane fuel cells(HTPEMFC),which possess high CO tolerance,easy thermal-water management,and high efficiency.However,conventional proton exchange membranes face the challenge of proton carrier leaking.To conquer such issue,in this work,the nanometer-sized proton transport channels are constructed in ZIF-8 membrane,in which deep eutectic solvents(DES)act as proton carriers to build continuous hydrogen-bonding networks.The microporous structure of ZIF-8 would inhibit DES leaking by size sieving.An excellent proton conductivity is achieved in the DES@ZIF-8 membrane,which is 1.21×10^(-2)S cm^(-1)at 180℃.Furthermore,it is discovered that weak electron donors could optimize the proton transport pathway by tuning the structure and HOMO of hydrogen-bonding networks,which would lower the activation energy for proton hopping and enhance rapid proton transport.As a proof of concept,the DES@ZIF-8 membrane is fabricated directly into a H_(2)/O_(2)HTPEMFC.This work provides not only a kind of efficient proton conducive electrolytes,but also theoretical support for improving proton conductivity by optimizing hydrogen-bonding networks.展开更多
Transport of vacuolar proteins from Golgi apparatus or trans-Golgi network （TGN） to vacuoles is a receptor-mediated process via an intermediate membrane-bound prevacuolar compartment （PVC） in plant cells. Both vac...Transport of vacuolar proteins from Golgi apparatus or trans-Golgi network （TGN） to vacuoles is a receptor-mediated process via an intermediate membrane-bound prevacuolar compartment （PVC） in plant cells. Both vacuolar sorting receptor （VSR） and receptor homology region-transmembrane domain-RING-H2 （RMR） proteins have been shown to function in transporting storage proteins to protein storage vacuole （PSV）, but little is known about the nature of the PVC for the PSV pathway. Here, we use the rice RMR1 （OsRMR1） as a probe to study the PSV pathway in plants. Immunogold electron microscopy （EM） with specific OsRMR1 antibodies showed that OsRMR1 proteins were found in the Golgi apparatus, TGN, and a distinct organelle with characteristics of PVC in both rice culture cells and developing rice seeds, as well as the protein body type II （PBII） or PSV in developing rice seeds. This organelle, also found in both tobacco BY-2 and Arabidopsis suspension cultured cells, is morphologically distinct from the VSR-positive multivesicular lytic PVC or multivesicular body （MVB） and thus represent a PVC for the PSV pathway that we name storage PVC （sPVC）. Further in vivo and in vitro interaction studies using truncated OsRMR1 proteins secreted into the culture media of transgenic BY-2 suspension cells demonstrated that OsRMR1 functions as a sorting receptor in transporting vicilin-like storage proteins.展开更多
Owing to the feature of strongα-glucosidase inhibitory activity,1-deoxynojirimycin(1-DNJ)has broad application prospects in areas of functional food,biomedicine,etc.,and this research wants to construct an efficient ...Owing to the feature of strongα-glucosidase inhibitory activity,1-deoxynojirimycin(1-DNJ)has broad application prospects in areas of functional food,biomedicine,etc.,and this research wants to construct an efficient strain for 1-DNJ production,basing on Bacillus amyloliquefaciens HZ-12.Firstly,using the temperature-sensitive shuttle plasmid T2(2)-Ori,gene ptsG in phosphotransferase system(PTS)was weakened by homologous recombination,and non-PTS pathway was strengthened by deleting its repressor gene iolR,and 1-DNJ yield of resultant strain HZ-S2 was increased by 4.27-fold,reached 110.72 mg/L.Then,to increase precursor fructose-6-phosphate(F-6-P)supply,phosphofructokinase was weaken,fructose phosphatase GlpX and 6-phosphate glucose isomerase Pgi were strengthened by promoter replacement,moreover,regulator gene nanR was deleted,1-DNJ yield was further increased to 267.37 mg/L by 2.41-fold.Subsequently,promoter of 1-DNJ synthetase cluster was optimized,as well as 5′-UTRs of downstream genes in synthetase cluster,and 1-DNJ produced by the final strain reached 478.62 mg/L.Last but not the least,1-DNJ yield of 1632.50 mg/L was attained in 3 L fermenter,which was the highest yield of 1-DNJ reported to date.Taken together,our results demonstrated that metabolic engineering was an effective strategy for 1-DNJ synthesis,this research laid a foundation for industrialization of functional food and drugs based on 1-DNJ.展开更多
基金supported by the National Natural Science Foundation of China(Nos.U19A2044,42105132,42030609,and 41975037)the National Key Research and Development Programof China(No.2022YFC3700303).
文摘Extreme ozone pollution events(EOPEs)are associated with synoptic weather patterns(SWPs)and pose severe health and ecological risks.However,a systematic investigation of themeteorological causes,transport pathways,and source contributions to historical EOPEs is still lacking.In this paper,the K-means clustering method is applied to identify six dominant SWPs during the warm season in the Yangtze River Delta(YRD)region from 2016 to 2022.It provides an integrated analysis of the meteorological factors affecting ozone pollution in Hefei under different SWPs.Using the WRF-FLEXPART model,the transport pathways(TPPs)and geographical sources of the near-surface air masses in Hefei during EOPEs are investigated.The results reveal that Hefei experienced the highest ozone concentration(134.77±42.82μg/m^(3)),exceedance frequency(46 days(23.23%)),and proportion of EOPEs(21 instances,47.7%)under the control of peripheral subsidence of typhoon(Type 5).Regional southeast winds correlated with the ozone pollution in Hefei.During EOPEs,a high boundary layer height,solar radiation,and temperature;lowhumidity and cloud cover;and pronounced subsidence airflow occurred over Hefei and the broader YRD region.The East-South(E_S)patterns exhibited the highest frequency(28 instances,65.11%).Regarding the TPPs and geographical sources of the near-surface air masses during historical EOPEs.The YRD was the main source for land-originating air masses under E_S patterns(50.28%),with Hefei,southern Anhui,southern Jiangsu,and northern Zhejiang being key contributors.These findings can help improve ozone pollution early warning and control mechanisms at urban and regional scales.
基金funded by the National Natural Science Foundation of China(grant No.42376246)the Key Research and Development Project of Guangxi(grant No.GuikeAB24010046)the Joint Funds of the National Natural Science Foundation of China(grant No.U2268217).
文摘In the context of urbanization,air pollution has emerged as a significant environmental challenge.A thorough understanding of their transport pathways,especially at a national scale,is essential for environmental protection and policy-making.However,it remains partially elusive due to the constraints of available data and analytical methods.This study proposed a data-driven spatiotemporal correlation analysis method employing the Dynamic Time Warping(DTW).We represented the first comprehensive attempt to chart the long-term and nationwide transport pathways of PM_(2.5) utilizing an extensive dataset spanning from 2000 to 2021 across China,which is crucial for understanding long-term air pollution trends.Compared with traditional chemical transport models(CTMs),this data-driven method can generate transport pathways of PM_(2.5) without requiring extensive meteorological or emission data,and suggesting fundamentally consistent spatial distribution and trends.Our analysis reveals that China’s transport pathways are notably pronounced in the Northwest(34%of the total pathways in China),Southwest(22%),and North(21%)regions,with less significant pathways in the Northeast(10%)region and isolated occurrences elsewhere.Additionally,a notable decrease in the number of China’s PM_(2.5) transport pathways,similar to annual average concentrations,was observed after 2013,aligning with stricter environmental regulations.Furthermore,we have demonstrated the feasibility of applying our method to the transport pathways of other gaseous pollutants.The approach is effective in detecting and quantifying air pollutants’transport pathways,even in regions like the Northwest with limited monitoring infrastructure,which may aid in environmental decision-making.The study will notably improve the current understanding of air pollutants’transport process,providing a new perspective for studying the large-scale spatiotemporal correlations.
基金supported by the National Natural Science Foundation of China(Grant 52102196 and 52402228)China Postdoctoral Science Foundation(Grant 2023M740474)Postdoctoral Fellowship Program of CPSF(GZB20230095)。
文摘Interface modification has been demonstrated as an effective means to enhance the performance of perovskite solar cells.However,the effect depends on the anchoring mode and strength of the interfacial molecules,which determines whether long-term robust interface for carrier viaduct can be achieved under operational light illumination.Herein,we select squaric acid(SA)as the interfacial molecule between the perovskite and SnO_(2)layer and propose a selfregulated bilateral anchoring strategy.The unique four-membered ring conjugated structure and dicarboxylic acid groups facilitate stable hydrogen bonds and coordination bonds at both SnO_(2)/SA and SA/PbI_(2)interfaces.The self-transforming property of SA enables the dynamic bilateral anchoring at the buried interface,ultimately releasing residual stress and constructing a stable interfacial molecular bridge.The results show that SA molecular bridge not only can effectively inhibit the generation of diverse charged defects but also serves as an effective electron transport pathway,resulting in improved power conversion efficiency(PCE)from 23.19 to 25.50%and excellent stability at the maximum power point.Additionally,the PCEs of the flexible and large-area(1 cm^(2))devices were increased to 24.92%and 24.01%,respectively,demonstrating the universal applicability of the bilateral anchoring to PSCs based on different substrates and larger area.
基金supported by the National Research Foundation of Korea(NRF-2022R1C1C1004171,RS-2024-00345331,and RS2024-00413975)supported by the 2025 Research Fund(1.250008.01)of UNIST。
文摘In situ X-ray diffraction(XRD)has been widely employed to probe the structural evolution of lithium-ion battery electrodes.However,electrodes with practically relevant high-energy densities often exhibit pronounced electrochemical inhomogeneities,which substantially hinder accurate structu ral interpretatio n.In this study,we systematically investigate the origin and characteristics of localized electrochemical dead zones(LEDZs)observed during in situ XRD measurements.Through comprehensive analyses using modified coin cells and pouch cells containing various cathode materials under diverse electrochemical conditions,we identify insufficient electron and ion conductivity as the prima ry cause of LEDZ formation.Crucially,our results demonstrate that decoupling electron and ion transport pathways from the X-ray beam direction-an effect inherently realized in pouch cell configurations-effectively mitigates LEDZs,thereby enabling artifact-free structural analysis.This work underscores the critical role of cell geometry and conductivity pathways in achieving accurate structural characterization,providing fundamental guidelines for the design and interpretation of high-energy-density lithium-ion battery electrodes.
基金supported by the National Natural Science Foundation of China(Nos.42121004,42275107,and 42077205)the National Key Research and Development Plan(No.2023YFC3706202)+1 种基金the Foundational and Applied Basic Research in Guangzhou in 2023(No.2023A04J0251)the Special Fund Project for Science and Technology Innovation Strategy of Guangdong Province(No.2019B121205004)。
文摘This study tracked the characteristics of atmospheric wet deposition of the toxic element arsenic(As)at both urban(Guangzhou(GZ))and forested(Dinghushan Natural Reserve(DHS))sites within the Pearl River Delta(PRD)region between 2016 and 2019,examining its correlation with rainfall patterns.Additionally,by employing backward trajectory analysis and the potential source contribution function(PSCF)in conjunction with pertinent emission inventories,we pinpointed the main pathways of atmospheric arsenic transport and evaluated the emission contributions from priority source areas.The study revealed that the atmospheric arsenic wet deposition fluxes at the GZ and DHS sites exhibited a trend of increase followed by a decrease over the four-year period.Wet season deposition fluxes were more than triple those of the dry season,with urban site showing a difference of over four times.Notably,wet season As deposition at both sites was predominantly affected by heavy rainfall from marine air masses,constituting 31%of the total deposition.The predominant trajectory directions contributing to arsenic deposition at GZ and DHS were northeast(55%)and south(53%),respectively.The primary source areas for both sites were largely outside the PRD region,with the GZ site having 80%to 95%of its source area in the non-PRD region,compared to 69%to 88%at the DHS site.Furthermore,non-PRD areas contributed approximately 65%to arsenic emissions for both sites,with the industrial sector being the dominant emission source,exceeding 97%of the total emissions.
基金supported by National Key Research and Development Plan(No.2017YFC0210100)the National Natural Science Foundation of China(Nos.41905086,41905107,42077205,41425020)+4 种基金the Special Fund Project for Science and Technology Innovation Strategy of Guangdong Province(No.2019B121205004)the Natural Science Foundation of Guangdong Province(No.2019A1515011291)the China Postdoctoral Science Foundation(No.2020M683174)the Air Quip(High resolution Air Quality Information for Policy)Project funded by the Research Council of Norway,the Collaborative Innovation Center of Climate Change,Jiangsu province,China,the high-performance computing platform of Jinan University,the Mt.Dinghu Forest Ecosystem Research Station,Chinese Academy of Sciences(CAS)the Comprehensive Observation and Study Site of Urban Meteorology and Environment,Sun Yat-sen University,and the Guangdong Province Key Laboratory for Climate Change and Natural Disaster Studies(No.2020B1212060025)
文摘This study attempts to identify the dominant transport pathways,potential source areas,and their seasonal variation at sites with high inorganic nitrogen(IN)wet deposition flux in southern China.This is a long-term study(2010-2017)based on continuous deposition measurements at the Guangzhou urban site(GZ)and the Dinghushan Natural Reserve site(DHS)located in the Pearl River Delta(PRD)region.A dataset on monthly IN concentration in precipitation and wet deposition flux were provided.The average annual fluxes measured at both sites(GZ:33.04±9.52,DHS:20.52±10.22 kg N/(ha·year))were higher,while the ratios of reduced to oxidized N(GZ:1.19±0.77,DHS:1.25±0.84)were lower compared with the national mean level and the previous reported level throughout the PRD region.The dominant pathways were not always consistent with the highest proportional trajectory clusters.The transport pathways contributing most of deposition were identified in the north and northnortheast in the dry season and in the east-southeast,east,and south-southwest in the wet season.A weighted potential source contribution function(WPSCF)value>0.3 was determined reasonably to define the potential source area.Emission within the PRD region contributed the majority(≥95%at both sites)of the IN deposition in the wet season,while the contribution outside the region increased significantly in the dry season(GZ:27.86%,DHS:95.26%).Our results could help create more effective policy to control precursor emissions for IN fluxes,enabling reduction of the ecological risks due to excessive nitrogen.
基金Supported by National Natural Science Foundation of China Enterprise Joint Fund Project(U24B200849)National Natural Science Foundation of China(91528303).
文摘Guided by the analysis of source-to-sink system,this study investigates the Paleogene Oligocene Lingshui Formation in the Qiongdongnan Basin by comparing the geological characterizes in land and sea areas and integrating outcrop,core,drilling,logging and 3D seismic data,to systematically analyze the characteristics of the source,transport pathway,and sink during the deposition of Lingshui Formation,and reveal the patterns,controlling factors and petroleum geologic significance of the source-to-sink systems.The results are obtained in five aspects.First,during the fault-depression transition,the Qiongdongnan Basin received sediments from the provenances presenting as segments in east-west and zones in north-south,primarily with the Indosinian granites in the Shenhu Uplift in the east and the Yanshanian granites in the west.Overall,the sources are young in the southern and northern parts and old in the interior of the basin.Second,three types of sediment transport pathways are identified:paleo-valleys,fault troughs and trough-valley transitional zones.Third,based on differences in sediment supply modes,the unique source-to-sink systems during the fault-depression transition in marine rift basins are categorized into three types:exogenous,endogenous and composite.Fourth,the characteristics of these source-to-sink systems are primarily controlled by provenance,paleogeomorphology,and sea-level changes.Provenance lithology and scale dictated the composition and volume of sedimentary deposits.Paleogeomorphology influenced erosion intensity in the provenance and the development of paleodrainage systems,thereby affecting the distribution and types of sedimentary systems.Additionally,sea-level changes decided the extent of the provenance,but also regulated the sediment distribution patterns through oceanic processes such as waves and tides.Fifth,the exogenous source-to-sink systems may form large-scale reservoir bodies,the endogenous systems develop secondary pores due to presence of soluble minerals,and the composite systems demonstrate reservoir properties varying from area to area.
基金supported by the National Key Research and Development Program of China(2020YFA0608503)the Science and Technology Program of Gansu Province(23ZDFA017).
文摘Regarding the rapid shrinkage of the Arctic cryosphere, sea ice plays a significant role in the temporal storage,transport, and release of microplastics(an emergent pollutant) among atmospheric, aquatic, and terrestrial environments. However, there are sparse studies on microplastics in the landfast sea ice and lagoon lake ice in the Alaskan Arctic region. Therefore, this study investigated characteristics and potential sources of microplastics in the landfast sea ice and lagoon lake ice in the Alaskan Arctic(Point Barrow). The results found that the average abundance of microplastics in the landfast sea ice(220.6±140.1 items/L) was comparable to that in lagoon lake ice near Point Barrow(148.9±141.8 items/L). For different layers of sea ice cores, the maximum abundance of microplastics generally occurred in the bottom layer. The overall particle sizes for the detected microplastics revealed that the abundance of microplastics decreased with increasing size for both landfast sea ice and lagoon lake ice samples. Small-sized microplastics(≤50 μm) accounted for more than 80% of the detected microplastics,with the dominant shape being fragments. The predominant polymers in sea ice were polyamide(PA), polyethylene(PE), and polyethylene terephthalate(PET). Meanwhile, PE and rubber dominated the polymers detected in lagoon lake ice. These differences between microplastics in Arctic sea ice and lagoon lake ice further indicated the discrepancies in microplastic transport pathways and deposition. Microplastics in landfast sea ice were mainly affected by seawater transported from the Pacific Ocean into the Chukchi Sea. In contrast, microplastics in lagoon lake ice were mostly influenced by the seawater of the Beaufort Sea and local vehicle emissions(e.g., rubber). This study further highlighted that a large abundance of microplastics was widely distributed in the sea ice of the Alaska Arctic region and may pose potential risks to the local ecosystems.
基金National Basic Research Program of China (Grant No. 2006CB400501)the Hundred Talents Program (Aerosol Characteristics and its Climatic Impact) of the Chinese Academy of Sciences, and National Natural Science Foun- dation of China (Grant Nos. 40675074, 40645028)
文摘Continuous observations of mass concentration and elemental composition of aerosol particles （PM2.5） were conducted at Tongyu, a semi-arid site in Northeast China in the spring of 2006. The average mass concentration of PM2.5 at Tongyu station was 260.9±274.4 μg m^-3 during the observation period. Nine dust events were monitored with a mean concentration of 528.0±302.7 μgm^-3. The PM2.5 level during non- dust storm （NDS） period was 111.65±63.37 μg m^-3. High mass concentration shows that fine-size particles pollution was very serious in the semi-arid area in Northeast China. The enrichment factor values for crust elements during the dust storm （DS） period are close to those in the NDS period, while the enrichment factor values for pollution elements during the NDS period are much higher than those in the DS period, showing these elements were from anthropogenic sources. The ratios of dust elements to Fe were relative constant during the DS period. The Ca/Fe ratio in dust aerosols at Tongyu is remarkably different from that observed in other source regions and downwind regions. Meteorological analysis shows that dust events at Tongyu are usually associated with dry, low pressure and high wind speed weather conditions. Air mass back-trajectory analysis identified three kinds of general pathways were associated with the aerosol particle transport to Tongyu, and the northwest direction pathway was the main transport route.
文摘The objective of this study was to characterize the mass concentration and chemical composition of aerosol particles（PM2.5） collected at Tongliao（Inner Mongolia Autonomous Region, China）, a site in Horqin Sand-land in northeast China. During spring 2005, the mass concentration for PM2.5 was （126±71）μg/m^3 in average. Five dust storm events were monitored with higher concentration of （255 ± 77）μg/m^3 in average than the non dusty days of （106 ± 44）μg/m^3. Concentrations for 20 elements were obtained by the PIXE method. Mass concentrations of ALl, Mg, Si, K, Ca, Ti, Mn, and V, which increased with the PM2.5 concentration, were higher than the pollution elements （S, Cl, Zn, Ar, Se, Br, and Pb）. Enrichment factor relative to crust material was also calculated, which showed dust trace elements were mainly from earth upper crust and pollution elements were dominated the anthropogenic aerosols. The Si/Al, Ca/Al, and Fe/Al ratios in PM2.5 samples at Tongliao were 4.07, 0.94, and 0.82, respectively, which were remarkably different with those on other source regions, such as ＂Western desert source region＂, ＂North desert source region＂ and central Asia source. Air mass back-trajectory analysis identified three kinds of general pathways were associated with the aerosol particle transport to Tongliao, but have the similar elemental ratios, implying that elemental signatures for dust aerosol from Horqin Sand-land were different with other regions.
基金The National Key Research and Development Program of China under contract No.2016YFA0601201the National Natural Science Foundation of China under contract Nos 91858202,91958203,41730533 and 41776003。
文摘This study presents a Lagrangian view of upper water exchanges across the Luzon Strait based on the finite-time Lyapunov exponents(FTLE)fields computed from the surface geostrophic current.The Lagrangian coherent structures(LCSs)extracted from the FTLE fields well identify the typical flow patterns and eddy activities around the Luzon Strait.In addition,they reveal the intricate transport paths and fluid domains,which are validated by the tracks of satellite-tracked surface drifters and cannot be visually recognized in the velocity maps.The FTLE fields indicate that there are mainly four types of transport patterns near the Luzon Strait;among them,the Kuroshio northward-flowing"leaping"pattern and the clockwise rotating"looping"pattern occur more frequently than the"leaking"pattern of the direct Kuroshio branch into the SCS and the"outflowing"pattern from the SCS to the Pacific.The eddy shedding events of the Kuroshio at the Luzon Strait are further analyzed,and the importance of considering LCSs in estimating transport by eddies is highlighted.The anticyclonic eddy(ACE)shedding cases reveal that ACEs mainly originate from the looping paths of Kuroshio and thus could effectively trap the Kuroshio water before eddy detachments.LCSs provide useful information to predict the positions of the upstream waters that finally enter the ACEs.In contrast,LCS snapshots indicate that during the formation of cyclonic eddies(CEs),most CEs are not connected with the pathways of Kuroshio water.Hence,the contribution of CEs to the surface water exchanges from the Pacific into the SCS is tiny.
基金supported by the Chinese Medicine Research Foundation of Jiangxi Provincial Health Department of China,No.2013A040the Science and Technology Program of Jiangxi Provincial Health Department of China,No.20123023the Science and Technology Support Program of Jiangxi Province of China,No.2009BSB11209
文摘Baicalin is a flavonoid compound extracted from Scutellaria baicalensis root.Recent evidence indicates that baicalin is neuroprotective in models of ischemic stroke.Here,we investigate the neuroprotective effect of baicalin in a neonatal rat model of hypoxic-ischemic encephalopathy.Seven-day-old pups underwent left common carotid artery ligation followed by hypoxia（8% oxygen at 37°C） for 2 hours,before being injected with baicalin（120 mg/kg intraperitoneally） and examined 24 hours later.Baicalin effectively reduced cerebral infarct volume and neuronal loss,inhibited apoptosis,and upregulated the expression of p-Akt and glutamate transporter 1.Intracerebroventricular injection of the phosphoinositide 3-kinase/protein kinase B（PI3 K/Akt） inhibitor LY294002 30 minutes before injury blocked the effect of baicalin on p-Akt and glutamate transporter 1,and weakened the associated neuroprotective effect.Our findings provide the first evidence,to our knowledge that baicalin can protect neonatal rat brains against hypoxic-ischemic injury by upregulating glutamate transporter 1 via the PI3 K/Akt signaling pathway.
基金supported by National Natural Science Foundation of China(No.81472478)Medical Science Youth Breeding Project of PLA(13QNP161)
文摘Objective To investigate the potential involvement of DMT1（IRE） protein in the brain vascular system in vivo during Pb exposure. Methods Three groups of male Sprague-Dawley rats were exposed to Pb in drinking water, among which two groups were concurrently administered by oral gavage once every other day as the low and high Fe treatment group, respectively, for 6 weeks. At the same time, the group only supplied with high Fe was also set as a reference. The animals were decapitated, then brain capillary-rich fraction was isolate from cerebral cortex. Western blot method was used to identify protein expression, and RT-PCR to detect the change of the m RNA. Results Pb exposure significantly increased Pb concentrations in cerebral cortex. Low Fe dose significantly reduced the cortex Pb levels, However, high Fe dose increased the cortex Pb levels. Interestingly, changes of DMT1（IRE） protein in brain capillary-rich fraction were highly related to the Pb level, but those of DMT1（IRE） m RNA were not significantly different. Moreover, the consistent changes in the levels of p-ERK1/2 or IRP1 with the changes in the levels of DMT1（IRE）. Conclusion These results suggest that Pb is transported into the brain through DMT1（IRE）, and the ERK MAPK pathway is involved in DMT1（IRE）-mediated transport regulation in brain vascular system in vivo.
基金support from European Union’s Horizon 2020 research,innovation programme under grant agreement No. 958174, Vinnova (Swedish Governmental Agency for Innovation Systems)the financial support from the LTU CREATERNITY program+2 种基金the J. Gust Richert Foundationthe Swedish Energy Agency,STINT (CH2019-8287),and Bio4energythe National Natural Science Foundation of China (No.U23A20122)。
文摘The confined ionic liquid(IL) in solid polymer composite electrolytes(SCPEs) can improve the performance of lithium metal batteries. However, the impact/role and working mechanism of confined IL in SCPEs remain ambiguous. Herein, IL was immobilized on SiO_(2)(SiO_(2)@IL-C) and then used to prepare the confined SCPEs together with LiTFSI and PEO to study the impacts of confined-IL on the properties and performance of electrolytes and reveal the Li+transport mechanism. The results show that, compared to the IL-unconfined SCPE, the IL-confined ones exhibit better performance of electrolytes and cells, such as higher ionic conductivity, higher t+Li, and wider electrochemical windows, as well as more stable cycle performance, due to the increased dissociation degree of lithium salt and enlarged polymer amorphousness. The finite-element/molecular-dynamics simulations suggest that the IL confined on the SiO_(2) provided an additional Li+transport pathway(Li+→ SiO_(2)@IL-C) that can accelerate ion transfer and alleviate lithium dendrites, leading to ultrastable stripping/plating cycling over 1900 h for the Li/SCPEs/Li symmetric cells. This study demonstrates that IL-confinement is an effective strategy for the intelligent approach of high-performance lithium metal batteries.
基金supported by the National Natural Science Foundation of China(Nos.22304027 and 81570807)the Natural Science Foundation of Fujian Province(No.2022J01208)+2 种基金the Joint Funds for the Innovation of Science and Technology,Fujian Province(Nos.2024Y9109,2024Y9107,and 2024Y9103)the Startup Fund for Scientific Research,Fujian Medical University(No.XRCZX2021015)the Scientific Research Foundation of State Key Laboratory of Vaccines for Infectious Diseases,and Xiang An Biomedicine Laboratory(No.2023XAKJ0101018).
文摘Intraocular injection of anti-vascular endothelial growth factor(VEGF)antibodies is the first-line treatment for ocular neovascular diseases.However,the invasive nature of this administration method often reduces patient compliance and negatively affects treatment outcomes.Noninvasive formulations of anti-VEGF antibody are urgently needed,but their development remains challenging due to the complex ocular barriers.This study identified an anti-VEGF singledomain antibody(sdVE01)that is three times smaller than the commercially available ranibizumab,yet retains a comparable anti-angiogenic effect to the heavy-chain region of ranibizumab(VHHL).Additionally,four dithiolane molecules(DM)were designed to construct DM-based antibody nanoformulations,which effectively penetrate both the anterior and posterior segments of the eye.Upon eyedrop administration,DM-based antibody nanoformulations significantly inhibited the VEGF pathway and reduced neovascularization in a corneal alkali-burn rat model.Notably,the therapeutic effects of the antibody eyedrops were comparable to those of ranibizumab administered via subconjunctival injection.Overall,the dithiolane-based antibody eyedrops represent a promising noninvasive strategy for treating ocular neovascularization diseases.
基金supported by the National Natural Science Foundation of China(22209060,22208126)the Natural Science Foundation of Jiangsu Province(BK20220539,BK20210782,BK20210774)+2 种基金the National Key R&D Program of China(2018YFE0121200)the China Postdoctoral Science Foundation(2023M731359,2024T170356)the Young Talent Support Fund from Jiangsu University(22JDG018)。
文摘The proton conductive electrolytes operating under high temperature(100-200℃)are highly desired for high temperature proton exchange membrane fuel cells(HTPEMFC),which possess high CO tolerance,easy thermal-water management,and high efficiency.However,conventional proton exchange membranes face the challenge of proton carrier leaking.To conquer such issue,in this work,the nanometer-sized proton transport channels are constructed in ZIF-8 membrane,in which deep eutectic solvents(DES)act as proton carriers to build continuous hydrogen-bonding networks.The microporous structure of ZIF-8 would inhibit DES leaking by size sieving.An excellent proton conductivity is achieved in the DES@ZIF-8 membrane,which is 1.21×10^(-2)S cm^(-1)at 180℃.Furthermore,it is discovered that weak electron donors could optimize the proton transport pathway by tuning the structure and HOMO of hydrogen-bonding networks,which would lower the activation energy for proton hopping and enhance rapid proton transport.As a proof of concept,the DES@ZIF-8 membrane is fabricated directly into a H_(2)/O_(2)HTPEMFC.This work provides not only a kind of efficient proton conducive electrolytes,but also theoretical support for improving proton conductivity by optimizing hydrogen-bonding networks.
文摘Transport of vacuolar proteins from Golgi apparatus or trans-Golgi network （TGN） to vacuoles is a receptor-mediated process via an intermediate membrane-bound prevacuolar compartment （PVC） in plant cells. Both vacuolar sorting receptor （VSR） and receptor homology region-transmembrane domain-RING-H2 （RMR） proteins have been shown to function in transporting storage proteins to protein storage vacuole （PSV）, but little is known about the nature of the PVC for the PSV pathway. Here, we use the rice RMR1 （OsRMR1） as a probe to study the PSV pathway in plants. Immunogold electron microscopy （EM） with specific OsRMR1 antibodies showed that OsRMR1 proteins were found in the Golgi apparatus, TGN, and a distinct organelle with characteristics of PVC in both rice culture cells and developing rice seeds, as well as the protein body type II （PBII） or PSV in developing rice seeds. This organelle, also found in both tobacco BY-2 and Arabidopsis suspension cultured cells, is morphologically distinct from the VSR-positive multivesicular lytic PVC or multivesicular body （MVB） and thus represent a PVC for the PSV pathway that we name storage PVC （sPVC）. Further in vivo and in vitro interaction studies using truncated OsRMR1 proteins secreted into the culture media of transgenic BY-2 suspension cells demonstrated that OsRMR1 functions as a sorting receptor in transporting vicilin-like storage proteins.
基金Key research and development program of Hubei Province(2022BBA0031)the Key Science and Technology Innovation Project of Hubei Province(2021BAD001)+1 种基金Wuhan Science and Technology Project(2020020602012124)Knowledge Innovation Program of Wuhan-Shuguang Project(2022020801020334).
文摘Owing to the feature of strongα-glucosidase inhibitory activity,1-deoxynojirimycin(1-DNJ)has broad application prospects in areas of functional food,biomedicine,etc.,and this research wants to construct an efficient strain for 1-DNJ production,basing on Bacillus amyloliquefaciens HZ-12.Firstly,using the temperature-sensitive shuttle plasmid T2(2)-Ori,gene ptsG in phosphotransferase system(PTS)was weakened by homologous recombination,and non-PTS pathway was strengthened by deleting its repressor gene iolR,and 1-DNJ yield of resultant strain HZ-S2 was increased by 4.27-fold,reached 110.72 mg/L.Then,to increase precursor fructose-6-phosphate(F-6-P)supply,phosphofructokinase was weaken,fructose phosphatase GlpX and 6-phosphate glucose isomerase Pgi were strengthened by promoter replacement,moreover,regulator gene nanR was deleted,1-DNJ yield was further increased to 267.37 mg/L by 2.41-fold.Subsequently,promoter of 1-DNJ synthetase cluster was optimized,as well as 5′-UTRs of downstream genes in synthetase cluster,and 1-DNJ produced by the final strain reached 478.62 mg/L.Last but not the least,1-DNJ yield of 1632.50 mg/L was attained in 3 L fermenter,which was the highest yield of 1-DNJ reported to date.Taken together,our results demonstrated that metabolic engineering was an effective strategy for 1-DNJ synthesis,this research laid a foundation for industrialization of functional food and drugs based on 1-DNJ.
