AIM:To investigate the role of genetic polymorphisms in the progression of hepatic fibrosis in hereditary haemochromatosis.METHODS:A cohort of 245 well-characterised C282Y homozygous patients with haemochromatosis was...AIM:To investigate the role of genetic polymorphisms in the progression of hepatic fibrosis in hereditary haemochromatosis.METHODS:A cohort of 245 well-characterised C282Y homozygous patients with haemochromatosis was studied,with all subjects having liver biopsy data and DNA available for testing.This study assessed the association of eight single nucleotide polymorphisms(SNPs)in a total of six genes including toll-like receptor 4(TLR4),transforming growth factor-beta(TGF-β),oxoguanine DNA glycosylase,monocyte chemoattractant protein 1,chemokine C-C motif receptor 2 and interleukin-10 with liver disease severity.Genotyping was performed using high resolution melt analysis and sequencing.The results were analysed in relation to the stage of hepatic fibrosis in multivariate analysis incorporating other cofactors including alcohol consumption and hepatic iron concentration.RESULTS:There were significant associations between the cofactors of male gender(P=0.0001),increasing age(P=0.006),alcohol consumption(P=0.0001),steatosis(P=0.03),hepatic iron concentration(P<0.0001)and the presence of hepatic fibrosis.Of the candidate gene polymorphisms studied,none showed a significant association with hepatic fibrosis in univariate or multivariate analysis incorporating cofactors.We also specifically studied patients with hepatic iron loading above threshold levels for cirrhosis and compared the genetic polymorphisms between those with no fibrosis vs cirrhosis however there was no significant effect from any of the candidate genes studied.Importantly,in this large,well characterised cohort of patients there was no association between SNPs for TGF-βor TLR4and the presence of fibrosis,cirrhosis or increasing fibrosis stage in multivariate analysis.CONCLUSION:In our large,well characterised group of haemochromatosis subjects we did not demonstrate any relationship between candidate gene polymorphisms and hepatic fibrosis or cirrhosis.展开更多
In-situ observations on α/γ phase transformation were made to study the effects of grain boundary microstructures on the formation of a new phase and the migration of α/γ interphase boundary in an iron4. 2%Cr allo...In-situ observations on α/γ phase transformation were made to study the effects of grain boundary microstructures on the formation of a new phase and the migration of α/γ interphase boundary in an iron4. 2%Cr alloy. It was found that triple junctions with more random boundaries could be the primary nucleation sites for a new phase, while triple junctions with low angle or low ∑ coincidence boundaries did not play any role as preferential sites. The migration of α/γ interphase boundary during heating over the transformation temperature range showed the two stage behaviour characterized by a stage with a migration velocity of 0. 33-0. 75 mm/s and secondly by a stage with 3. 7-7. 6 mm/s. It was also found that abnormal grain growth and a high density of ∑3 coincidence boundaries could occur in a phase with bcc structure after cycling of α/γ phase transformation. A new mechanism of nucleation and growth of a new phase in α/γ phase transformation is proposed on the basis of roles of plane-matching interphase boundaries, as previously discussed on the origin of anisotropy of grain growth due to the migration of {110} plane-matching boundaries in Fe-3z%Si alloy. The most recent theoretical work on the distribution of plane-matching boundaries in solids with different crystal structures was found to be useful for the understanding of nucleation and growth during α/γ phase transformation.展开更多
We reported in this manuscript that TGF-beta1 induces apoptosis in AML12 murine hepatocytes, which is associated with the activation of p38 MAPK signaling pathway. SB202190, a specific inhibitor of p38 MAPK, strongly ...We reported in this manuscript that TGF-beta1 induces apoptosis in AML12 murine hepatocytes, which is associated with the activation of p38 MAPK signaling pathway. SB202190, a specific inhibitor of p38 MAPK, strongly inhibited the TGF-beta1-induced apoptosis and PAI-1 promoter activity. Treatment of cells with TGF-beta1 activates p38. Furthermore, over-expression of dominant negative mutant p38 also reduced the TGF-beta1-induced apoptosis. The data indicate that the activation of p38 is involved in TGF-beta1-mediated gene expression and apoptosis.展开更多
Taking Zhejiang Province as an example,this paper explores the mechanisms and implementation pathways through which the low-altitude economy drives the transformation and upgrading of the tourism industry.It finds tha...Taking Zhejiang Province as an example,this paper explores the mechanisms and implementation pathways through which the low-altitude economy drives the transformation and upgrading of the tourism industry.It finds that the low-altitude economy can effectively promote the development of high-end and diversified tourism in Zhejiang by innovating tourism formats,optimizing resource allocation,and enhancing tourist experiences.Besides,it analyzes the current development status of the low-altitude economy in Zhejiang and its potential for integration with tourism,revealing specific enabling pathways for tourism transformation,including low-altitude sightseeing,aviation tourism,and low-altitude sports.Finally,it proposes policy recommendations such as strengthening policy support,enhancing infrastructure development,and cultivating market entities.The findings aim to provide theoretical references and practical guidance for the high-quality development of tourism in Zhejiang Province.展开更多
Image registration is an indispensable component in multi-source remote sensing image processing. In this paper, we put forward a remote sensing image registration method by including an improved multi-scale and multi...Image registration is an indispensable component in multi-source remote sensing image processing. In this paper, we put forward a remote sensing image registration method by including an improved multi-scale and multi-direction Harris algorithm and a novel compound feature. Multi-scale circle Gaussian combined invariant moments and multi-direction gray level co-occurrence matrix are extracted as features for image matching. The proposed algorithm is evaluated on numerous multi-source remote sensor images with noise and illumination changes. Extensive experimental studies prove that our proposed method is capable of receiving stable and even distribution of key points as well as obtaining robust and accurate correspondence matches. It is a promising scheme in multi-source remote sensing image registration.展开更多
The problem of estimating an image corrupted by additive white Gaussian noise has been of interest for practical reasons. Non-linear denoising methods based on wavelets, have become popular but Multiwavelets outperfor...The problem of estimating an image corrupted by additive white Gaussian noise has been of interest for practical reasons. Non-linear denoising methods based on wavelets, have become popular but Multiwavelets outperform wavelets in image denoising. Multiwavelets are wavelets with several scaling and wavelet functions, offer simultaneously Orthogonality, Symmetry, Short support and Vanishing moments, which is not possible with ordinary (scalar) wavelets. These properties make Multiwavelets promising for image processing applications, such as image denoising. The aim of this paper is to apply various non-linear thresholding techniques such as hard, soft, universal, modified universal, fixed and multivariate thresholding in Multiwavelet transform domain such as Discrete Multiwavelet Transform, Symmetric Asymmetric (SA4), Chui Lian (CL), and Bi-Hermite (Bih52S) for different Multiwavelets at different levels, to denoise an image and determine the best one out of it. The performance of denoising algorithms and various thresholding are measured using quantitative performance measures such as, Mean Square Error (MSE), and Root Mean Square Error (RMSE), Signal-to-Noise Ratio (SNR), Peak Signal-to-Noise Ratio (PSNR). It is found that CL Multiwavelet transform in combination with modified universal thresholding has given best results.展开更多
The transition from a known Taylor series ?of a known function f(x) to a new function ?primarily defined by the infinite power series ?with coefficients f(n)(0)?from the Taylor series of the function f(x)?can be made ...The transition from a known Taylor series ?of a known function f(x) to a new function ?primarily defined by the infinite power series ?with coefficients f(n)(0)?from the Taylor series of the function f(x)?can be made by an integral transformation which is a modified Laplace transformation and is called Sumudu transformation. It makes the transition from the Exponential series to the Geometric series and may help to evaluate new infinite power series from known Taylor series. The Sumudu transformation is demonstrated to be a limiting case of Fractional integration. Apart from the basic Sumudu integral transformation we discuss a modification where the coefficients ?from the Taylor series are not changed to f(n)(0)?but only to . Beside simple examples our applications are mainly concerned to calculate new Generating functions for Hermite polynomials from the basic ones.展开更多
Background: To study the influence of blood lipid levels on hemorrhagic transformation(HT) and prognosis after acute cerebral infarction(ACI).Methods: Patients with ACI within 72 h of symptoms onset between January 1 ...Background: To study the influence of blood lipid levels on hemorrhagic transformation(HT) and prognosis after acute cerebral infarction(ACI).Methods: Patients with ACI within 72 h of symptoms onset between January 1 st, 2015, and December 31 st, 2016, were retrospectively analyzed. Patients were divided into group A(without HT) and group B(HT). The outcomes were assessed after 3 months of disease onset using the modified Rankin Scale(m RS). An m RS score of 0–2 points indicated excellent prognosis, and an m RS score of 3–6 points indicated poor prognosis.Results: A total of 732 patients conformed to the inclusion criteria, including 628 in group A and 104 in group B. The incidence of HT was 14.2%, and the median onset time was 2 d(interquartile range, 1–7 d). The percentages of patients with large infarct size and cortex involvement in group B were 80.8% and 79.8%, respectively, which were both significantly higher than those in group A(28.7 and 33.4%, respectively). The incidence rate of atrial fibrillation(AF) in group B was significantly higher than that in group A(39.4% vs. 13.9%, P<0.001). The adjusted multivariate analysis results showed that large infarct size, cortex involvement and AF were independent risk factors of HT, while total cholesterol(TC) was a protective factor of HT(OR=0.359, 95% CI 0.136–0.944, P=0.038). With every 1 mmol/L reduction in normal TC levels, the risk of HT increased by 64.1%. The mortality and morbidity at 3 months in group B(21.2% and 76.7%, respectively) were both significantly higher than those in group A(8.0% and 42.8%, respectively). The adjusted multivariate analysis results showed that large infarct size(OR=12.178, 95% CI 5.390–27.516, P<0.001) was an independent risk factor of long-term unfavorable outcomes, whereas low-density lipoprotein cholesterol(LDL-C) was a protective factor(OR=0.538, 95% CI 0.300–0.964, P=0.037). With every 1 mmol/L reduction in normal LDL-C levels, the risk of an unfavorable outcome increased by 46.2%. Major therapies, including intravenous recombinant human tissue plasminogen activator(r TPA), intensive lipid-lowering statins and anti-platelets, were not significantly related to either HT or long-term, post-ACI poor prognosis.Conclusions: For patients with large infarct sizes, especially those with cortex involvement, AF, or lower levels of TC, the risk of HT might increase after ACI. The risk of a long-term unfavorable outcome in these patients might increase with a reduction in LDL-C.展开更多
It is shown that in Euclidean space with any number of spatial dimensions more than three, the Lorentz transform holds true if the proper time of each elementary particle is proportional to the length of its path in t...It is shown that in Euclidean space with any number of spatial dimensions more than three, the Lorentz transform holds true if the proper time of each elementary particle is proportional to the length of its path in the extra-dimensional subspace, and all elementary particles move at the speed of light in the complete space. The six-dimensional treatment of the Coulomb force of interaction between two charges is given. The electric force is due to the motion of charges in the extra-dimensional subspace and is equal to the corresponding Lorentz force.展开更多
AIM: To study persistence and replication of hepatitis C virus (HCV) in patients' peripheral blood mononuclear cells (PBMC) cultured in vitro. METHODS: Epstein Barr virus (EBV) was used to transform the hepatitis ...AIM: To study persistence and replication of hepatitis C virus (HCV) in patients' peripheral blood mononuclear cells (PBMC) cultured in vitro. METHODS: Epstein Barr virus (EBV) was used to transform the hepatitis C virus from a HCV positive patient to permanent lymphoblastoid cell lines (LCL). Positive and negative HCV RNA strands of the cultured cells and growth media were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) each month. Core and NS5 proteins of HCV were further tested using immunohistochemical SP method and in situ RT-PCR. RESULTS: HCV RNA positive strands were consistently detected the cultured cells for one year. The negative-strand RNA in LCL cells and the positive-strand RNA in supernatants were observed intermittently. Immunohistochemical results medicated expression of HCV NS3 and C proteins in LCL cytoplasm mostly. The positive signal of PCR product was dark blue and mainly localized to the LCL cytoplasm. The RT-PCR signal was eliminated by overnight RNase digestion but not DNase digestion. CONCLUSION: HCV may exist and remain functional in a cultured cell line for a long period.展开更多
In the Kigongo area of Mwanza Region,northwest Tanzania,fishmonger Neema Aisha remembers how the morning’s fresh catch would sour while she queued for the ferry,putting her business at risk.
Adult neurogenesis continuously produces new neurons critical for cognitive plasticity in adult rodents.While it is known transforming growth factor-βsignaling is important in embryonic neurogenesis,its role in postn...Adult neurogenesis continuously produces new neurons critical for cognitive plasticity in adult rodents.While it is known transforming growth factor-βsignaling is important in embryonic neurogenesis,its role in postnatal neurogenesis remains unclear.In this study,to define the precise role of transforming growth factor-βsignaling in postnatal neurogenesis at distinct stages of the neurogenic cascade both in vitro and in vivo,we developed two novel inducible and cell type-specific mouse models to specifically silence transforming growth factor-βsignaling in neural stem cells in(mGFAPcre-ALK5fl/fl-Ai9)or immature neuroblasts in(DCXcreERT2-ALK5fl/fl-Ai9).Our data showed that exogenous transforming growth factor-βtreatment led to inhibition of the proliferation of primary neural stem cells while stimulating their migration.These effects were abolished in activin-like kinase 5(ALK5)knockout primary neural stem cells.Consistent with this,inhibition of transforming growth factor-βsignaling with SB-431542 in wild-type neural stem cells stimulated proliferation while inhibited the migration of neural stem cells.Interestingly,deletion of transforming growth factor-βreceptor in neural stem cells in vivo inhibited the migration of postnatal born neurons in mGFAPcre-ALK5fl/fl-Ai9 mice,while abolishment of transforming growth factor-βsignaling in immature neuroblasts in DCXcreERT2-ALK5fl/fl-Ai9 mice did not affect the migration of these cells in the hippocampus.In summary,our data supports a dual role of transforming growth factor-βsignaling in the proliferation and migration of neural stem cells in vitro.Moreover,our data provides novel insights on cell type-specific-dependent requirements of transforming growth factor-βsignaling on neural stem cell proliferation and migration in vivo.展开更多
In this work, a new method to deal with the unconnected pixels in motion compensated temporal filtering (MCTF) is presented, which is designed to improve the performance of 3D lifted wavelet coding. Furthermore, multi...In this work, a new method to deal with the unconnected pixels in motion compensated temporal filtering (MCTF) is presented, which is designed to improve the performance of 3D lifted wavelet coding. Furthermore, multiple description scalable coding (MDSC) is investigated, and novel MDSC schemes based on 3D wavelet coding are proposed, using the lifting imple- mentation of temporal filtering. The proposed MDSC schemes can avoid the mismatch problem in multiple description video coding, and have high scalability and robustness of video transmission. Experimental results showed that the proposed schemes are feasible and adequately effective.展开更多
OBJECTIVE:To evaluate the effect of Wulong Xiaozheng Wan medicated serum on the epithelial-mesenchymal transition (EMT) of BGC823 cell induced by transforming growth factor-β,(TGF-β,) and to explore its mechanism.ME...OBJECTIVE:To evaluate the effect of Wulong Xiaozheng Wan medicated serum on the epithelial-mesenchymal transition (EMT) of BGC823 cell induced by transforming growth factor-β,(TGF-β,) and to explore its mechanism.METHODS:EMT model of BGC823 was stimulated by TGF-β1.Wulong Xiaozheng Wan medicated serum and LY-364947 were used as intervention.The proliferation and adhesion of BGC823 were detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide and flow cytometry was used to detect the apoptosis.The invasion and migration were detected by Transwell.The level of matrix metalloproteins was detected by enzyme-linked immunosorbent assay.The expressions of related proteins and mRNA of EMT marker and TGF-β1/Smad signal pathway were detected by Western blot and reverse transcription-polymerase chain reaction.RESULTS:Compared with the TGF-β1 group,Wulong Xiaozheng Wan medicated serum could inhibit the ability of proliferation,heterogeneous adhesion,invasion,and migration.It also promotes apoptosis and homotypic adhesion in BGC823,with a dose-dependent manner.Meanwhile,Wulong Xiaozheng Wan medicated serum could regulate the expression of related proteins and mRNA of TGF-β1/Smad signaling pathway,and inhibit the expressions of EMT transcription factors and EMT markers.CONCLUSION:Wulong Xiaozheng Wan medicated serum inhibited epithelial-mesenchymal transition by down-regulated the expression of TβRI and the activation of TGF-β1/Smad signaling pathway.展开更多
In this brief communication we present a new integral transform, so far unknown, which is applicable, for instance, to studying the kinetic theory of natural eigenmodes or transport excited in plasmas with bounded dis...In this brief communication we present a new integral transform, so far unknown, which is applicable, for instance, to studying the kinetic theory of natural eigenmodes or transport excited in plasmas with bounded distribution functions such as in Q machines/plasma diodes or in the scrap-off layer of Tokamak fusion plasmas. The results are valid for functions of function spaces—Lebesgue spaces, which are defined using a natural generalization of the p-norm for finite-dimensional vector spaces, where is the real set, σs is the σ-algebra of Lebesgue measurable sets, and the μ Lebesgue measure. , so that . Note that, using a simpler notation, more natural/known to engineers, f could be considered any piecewise continuous function, that is: Here is a Euclidian space with the usual norm (inner product: ) given by: [1].展开更多
Background:Accurate classification of normal blood cells is a critical foundation for automated hematological analysis,including the detection of pathological conditions like leukemia.While convolutional neural networ...Background:Accurate classification of normal blood cells is a critical foundation for automated hematological analysis,including the detection of pathological conditions like leukemia.While convolutional neural networks(CNNs)excel in local feature extraction,their ability to capture global contextual relationships in complex cellular morphologies is limited.This study introduces a hybrid CNN-Transformer framework to enhance normal blood cell classification,laying the groundwork for future leukemia diagnostics.Methods:The proposed architecture integrates pre-trained CNNs(ResNet50,EfficientNetB3,InceptionV3,CustomCNN)with Vision Transformer(ViT)layers to combine local and global feature modeling.Four hybrid models were evaluated on the publicly available Blood Cell Images dataset from Kaggle,comprising 17,092 annotated normal blood cell images across eight classes.The models were trained using transfer learning,fine-tuning,and computational optimizations,including cross-model parameter sharing to reduce redundancy by reusing weights across CNN backbones and attention-guided layer pruning to eliminate low-contribution layers based on attention scores,improving efficiency without sacrificing accuracy.Results:The InceptionV3-ViT model achieved a weighted accuracy of 97.66%(accounting for class imbalance by weighting each class’s contribution),a macro F1-score of 0.98,and a ROC-AUC of 0.998.The framework excelled in distinguishing morphologically similar cell types demonstrating robustness and reliable calibration(ECE of 0.019).The framework addresses generalization challenges,including class imbalance and morphological similarities,ensuring robust performance across diverse cell types.Conclusion:The hybrid CNN-Transformer framework significantly improves normal blood cell classification by capturing multi-scale features and long-range dependencies.Its high accuracy,efficiency,and generalization position it as a strong baseline for automated hematological analysis,with potential for extension to leukemia subtype classification through future validation on pathological samples.展开更多
An effective near-field - far-field (NF - FF) transformation with spherical scanning for quasi-planar antennas from irregularly spaced data is developed in this paper. Two efficient approaches for evaluating the regul...An effective near-field - far-field (NF - FF) transformation with spherical scanning for quasi-planar antennas from irregularly spaced data is developed in this paper. Two efficient approaches for evaluating the regularly spaced spherical samples from the nonuniformly distributed ones are proposed and numerically compared. Both the approaches rely on a nonredundant sampling representation of the voltage measured by the probe, based on an oblate ellipsoidal modelling of the antenna under test. The former employs the singular value decomposition method to reconstruct the NF data at the points fixed by the nonredundant sampling representation and can be applied when the irregularly acquired samples lie on nonuniform parallels. The latter is based on an iterative technique and can be used also when such a hypothesis does not hold, but requires the existence of a biunique correspondence between the uniform and nonuniform samples, associ- ating at each uniform sampling point the nearest irregular one. Once the regularly spaced spherical samples have been recovered, the NF data needed by a probe compensated NF - FF transformation with spherical scanning are efficiently evaluated by using an optimal sampling interpolation algorithm. It is so possible to accurately compensate known posi- tioning errors in the NF - FF transformation with spherical scanning for quasi-planar antennas. Some numerical tests assessing the accuracy and the robustness of the proposed approaches are reported.展开更多
基金Supported by NHMRC Medical Postgraduate Scholarship and the Royal Brisbane and Women’s Hospital Research Foundation to Wood MJthe National Health and Medical Research Council(NHMRC)to Ramm GA and Powell LW+1 种基金the recipient of an NHMRC Senior Research Fellowship,1024672 to Subramaniam VNan NHMRC Senior Research Fellowship,No.552409 to Ramm GA
文摘AIM:To investigate the role of genetic polymorphisms in the progression of hepatic fibrosis in hereditary haemochromatosis.METHODS:A cohort of 245 well-characterised C282Y homozygous patients with haemochromatosis was studied,with all subjects having liver biopsy data and DNA available for testing.This study assessed the association of eight single nucleotide polymorphisms(SNPs)in a total of six genes including toll-like receptor 4(TLR4),transforming growth factor-beta(TGF-β),oxoguanine DNA glycosylase,monocyte chemoattractant protein 1,chemokine C-C motif receptor 2 and interleukin-10 with liver disease severity.Genotyping was performed using high resolution melt analysis and sequencing.The results were analysed in relation to the stage of hepatic fibrosis in multivariate analysis incorporating other cofactors including alcohol consumption and hepatic iron concentration.RESULTS:There were significant associations between the cofactors of male gender(P=0.0001),increasing age(P=0.006),alcohol consumption(P=0.0001),steatosis(P=0.03),hepatic iron concentration(P<0.0001)and the presence of hepatic fibrosis.Of the candidate gene polymorphisms studied,none showed a significant association with hepatic fibrosis in univariate or multivariate analysis incorporating cofactors.We also specifically studied patients with hepatic iron loading above threshold levels for cirrhosis and compared the genetic polymorphisms between those with no fibrosis vs cirrhosis however there was no significant effect from any of the candidate genes studied.Importantly,in this large,well characterised cohort of patients there was no association between SNPs for TGF-βor TLR4and the presence of fibrosis,cirrhosis or increasing fibrosis stage in multivariate analysis.CONCLUSION:In our large,well characterised group of haemochromatosis subjects we did not demonstrate any relationship between candidate gene polymorphisms and hepatic fibrosis or cirrhosis.
文摘In-situ observations on α/γ phase transformation were made to study the effects of grain boundary microstructures on the formation of a new phase and the migration of α/γ interphase boundary in an iron4. 2%Cr alloy. It was found that triple junctions with more random boundaries could be the primary nucleation sites for a new phase, while triple junctions with low angle or low ∑ coincidence boundaries did not play any role as preferential sites. The migration of α/γ interphase boundary during heating over the transformation temperature range showed the two stage behaviour characterized by a stage with a migration velocity of 0. 33-0. 75 mm/s and secondly by a stage with 3. 7-7. 6 mm/s. It was also found that abnormal grain growth and a high density of ∑3 coincidence boundaries could occur in a phase with bcc structure after cycling of α/γ phase transformation. A new mechanism of nucleation and growth of a new phase in α/γ phase transformation is proposed on the basis of roles of plane-matching interphase boundaries, as previously discussed on the origin of anisotropy of grain growth due to the migration of {110} plane-matching boundaries in Fe-3z%Si alloy. The most recent theoretical work on the distribution of plane-matching boundaries in solids with different crystal structures was found to be useful for the understanding of nucleation and growth during α/γ phase transformation.
基金grants fromthe Chinese Academy of Sciences (No. KJ951-BI608), the National Natural Sciences FOundation ofChina (No. 39625007 and
文摘We reported in this manuscript that TGF-beta1 induces apoptosis in AML12 murine hepatocytes, which is associated with the activation of p38 MAPK signaling pathway. SB202190, a specific inhibitor of p38 MAPK, strongly inhibited the TGF-beta1-induced apoptosis and PAI-1 promoter activity. Treatment of cells with TGF-beta1 activates p38. Furthermore, over-expression of dominant negative mutant p38 also reduced the TGF-beta1-induced apoptosis. The data indicate that the activation of p38 is involved in TGF-beta1-mediated gene expression and apoptosis.
文摘Taking Zhejiang Province as an example,this paper explores the mechanisms and implementation pathways through which the low-altitude economy drives the transformation and upgrading of the tourism industry.It finds that the low-altitude economy can effectively promote the development of high-end and diversified tourism in Zhejiang by innovating tourism formats,optimizing resource allocation,and enhancing tourist experiences.Besides,it analyzes the current development status of the low-altitude economy in Zhejiang and its potential for integration with tourism,revealing specific enabling pathways for tourism transformation,including low-altitude sightseeing,aviation tourism,and low-altitude sports.Finally,it proposes policy recommendations such as strengthening policy support,enhancing infrastructure development,and cultivating market entities.The findings aim to provide theoretical references and practical guidance for the high-quality development of tourism in Zhejiang Province.
基金supported by National Nature Science Foundation of China (Nos. 61462046 and 61762052)Natural Science Foundation of Jiangxi Province (Nos. 20161BAB202049 and 20161BAB204172)+2 种基金the Bidding Project of the Key Laboratory of Watershed Ecology and Geographical Environment Monitoring, NASG (Nos. WE2016003, WE2016013 and WE2016015)the Science and Technology Research Projects of Jiangxi Province Education Department (Nos. GJJ160741, GJJ170632 and GJJ170633)the Art Planning Project of Jiangxi Province (Nos. YG2016250 and YG2017381)
文摘Image registration is an indispensable component in multi-source remote sensing image processing. In this paper, we put forward a remote sensing image registration method by including an improved multi-scale and multi-direction Harris algorithm and a novel compound feature. Multi-scale circle Gaussian combined invariant moments and multi-direction gray level co-occurrence matrix are extracted as features for image matching. The proposed algorithm is evaluated on numerous multi-source remote sensor images with noise and illumination changes. Extensive experimental studies prove that our proposed method is capable of receiving stable and even distribution of key points as well as obtaining robust and accurate correspondence matches. It is a promising scheme in multi-source remote sensing image registration.
文摘The problem of estimating an image corrupted by additive white Gaussian noise has been of interest for practical reasons. Non-linear denoising methods based on wavelets, have become popular but Multiwavelets outperform wavelets in image denoising. Multiwavelets are wavelets with several scaling and wavelet functions, offer simultaneously Orthogonality, Symmetry, Short support and Vanishing moments, which is not possible with ordinary (scalar) wavelets. These properties make Multiwavelets promising for image processing applications, such as image denoising. The aim of this paper is to apply various non-linear thresholding techniques such as hard, soft, universal, modified universal, fixed and multivariate thresholding in Multiwavelet transform domain such as Discrete Multiwavelet Transform, Symmetric Asymmetric (SA4), Chui Lian (CL), and Bi-Hermite (Bih52S) for different Multiwavelets at different levels, to denoise an image and determine the best one out of it. The performance of denoising algorithms and various thresholding are measured using quantitative performance measures such as, Mean Square Error (MSE), and Root Mean Square Error (RMSE), Signal-to-Noise Ratio (SNR), Peak Signal-to-Noise Ratio (PSNR). It is found that CL Multiwavelet transform in combination with modified universal thresholding has given best results.
文摘The transition from a known Taylor series ?of a known function f(x) to a new function ?primarily defined by the infinite power series ?with coefficients f(n)(0)?from the Taylor series of the function f(x)?can be made by an integral transformation which is a modified Laplace transformation and is called Sumudu transformation. It makes the transition from the Exponential series to the Geometric series and may help to evaluate new infinite power series from known Taylor series. The Sumudu transformation is demonstrated to be a limiting case of Fractional integration. Apart from the basic Sumudu integral transformation we discuss a modification where the coefficients ?from the Taylor series are not changed to f(n)(0)?but only to . Beside simple examples our applications are mainly concerned to calculate new Generating functions for Hermite polynomials from the basic ones.
文摘Background: To study the influence of blood lipid levels on hemorrhagic transformation(HT) and prognosis after acute cerebral infarction(ACI).Methods: Patients with ACI within 72 h of symptoms onset between January 1 st, 2015, and December 31 st, 2016, were retrospectively analyzed. Patients were divided into group A(without HT) and group B(HT). The outcomes were assessed after 3 months of disease onset using the modified Rankin Scale(m RS). An m RS score of 0–2 points indicated excellent prognosis, and an m RS score of 3–6 points indicated poor prognosis.Results: A total of 732 patients conformed to the inclusion criteria, including 628 in group A and 104 in group B. The incidence of HT was 14.2%, and the median onset time was 2 d(interquartile range, 1–7 d). The percentages of patients with large infarct size and cortex involvement in group B were 80.8% and 79.8%, respectively, which were both significantly higher than those in group A(28.7 and 33.4%, respectively). The incidence rate of atrial fibrillation(AF) in group B was significantly higher than that in group A(39.4% vs. 13.9%, P<0.001). The adjusted multivariate analysis results showed that large infarct size, cortex involvement and AF were independent risk factors of HT, while total cholesterol(TC) was a protective factor of HT(OR=0.359, 95% CI 0.136–0.944, P=0.038). With every 1 mmol/L reduction in normal TC levels, the risk of HT increased by 64.1%. The mortality and morbidity at 3 months in group B(21.2% and 76.7%, respectively) were both significantly higher than those in group A(8.0% and 42.8%, respectively). The adjusted multivariate analysis results showed that large infarct size(OR=12.178, 95% CI 5.390–27.516, P<0.001) was an independent risk factor of long-term unfavorable outcomes, whereas low-density lipoprotein cholesterol(LDL-C) was a protective factor(OR=0.538, 95% CI 0.300–0.964, P=0.037). With every 1 mmol/L reduction in normal LDL-C levels, the risk of an unfavorable outcome increased by 46.2%. Major therapies, including intravenous recombinant human tissue plasminogen activator(r TPA), intensive lipid-lowering statins and anti-platelets, were not significantly related to either HT or long-term, post-ACI poor prognosis.Conclusions: For patients with large infarct sizes, especially those with cortex involvement, AF, or lower levels of TC, the risk of HT might increase after ACI. The risk of a long-term unfavorable outcome in these patients might increase with a reduction in LDL-C.
文摘It is shown that in Euclidean space with any number of spatial dimensions more than three, the Lorentz transform holds true if the proper time of each elementary particle is proportional to the length of its path in the extra-dimensional subspace, and all elementary particles move at the speed of light in the complete space. The six-dimensional treatment of the Coulomb force of interaction between two charges is given. The electric force is due to the motion of charges in the extra-dimensional subspace and is equal to the corresponding Lorentz force.
基金The paper was support by a grant from the Ministry Youth Research of China,No.98-1-269
文摘AIM: To study persistence and replication of hepatitis C virus (HCV) in patients' peripheral blood mononuclear cells (PBMC) cultured in vitro. METHODS: Epstein Barr virus (EBV) was used to transform the hepatitis C virus from a HCV positive patient to permanent lymphoblastoid cell lines (LCL). Positive and negative HCV RNA strands of the cultured cells and growth media were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) each month. Core and NS5 proteins of HCV were further tested using immunohistochemical SP method and in situ RT-PCR. RESULTS: HCV RNA positive strands were consistently detected the cultured cells for one year. The negative-strand RNA in LCL cells and the positive-strand RNA in supernatants were observed intermittently. Immunohistochemical results medicated expression of HCV NS3 and C proteins in LCL cytoplasm mostly. The positive signal of PCR product was dark blue and mainly localized to the LCL cytoplasm. The RT-PCR signal was eliminated by overnight RNase digestion but not DNase digestion. CONCLUSION: HCV may exist and remain functional in a cultured cell line for a long period.
文摘In the Kigongo area of Mwanza Region,northwest Tanzania,fishmonger Neema Aisha remembers how the morning’s fresh catch would sour while she queued for the ferry,putting her business at risk.
基金supported by NIH grants,Nos.R01NS125074,R01AG083164,R01NS107365,and R21NS127177(to YL),1F31NS129204-01A1(to KW)and Albert Ryan Fellowship(to KW).
文摘Adult neurogenesis continuously produces new neurons critical for cognitive plasticity in adult rodents.While it is known transforming growth factor-βsignaling is important in embryonic neurogenesis,its role in postnatal neurogenesis remains unclear.In this study,to define the precise role of transforming growth factor-βsignaling in postnatal neurogenesis at distinct stages of the neurogenic cascade both in vitro and in vivo,we developed two novel inducible and cell type-specific mouse models to specifically silence transforming growth factor-βsignaling in neural stem cells in(mGFAPcre-ALK5fl/fl-Ai9)or immature neuroblasts in(DCXcreERT2-ALK5fl/fl-Ai9).Our data showed that exogenous transforming growth factor-βtreatment led to inhibition of the proliferation of primary neural stem cells while stimulating their migration.These effects were abolished in activin-like kinase 5(ALK5)knockout primary neural stem cells.Consistent with this,inhibition of transforming growth factor-βsignaling with SB-431542 in wild-type neural stem cells stimulated proliferation while inhibited the migration of neural stem cells.Interestingly,deletion of transforming growth factor-βreceptor in neural stem cells in vivo inhibited the migration of postnatal born neurons in mGFAPcre-ALK5fl/fl-Ai9 mice,while abolishment of transforming growth factor-βsignaling in immature neuroblasts in DCXcreERT2-ALK5fl/fl-Ai9 mice did not affect the migration of these cells in the hippocampus.In summary,our data supports a dual role of transforming growth factor-βsignaling in the proliferation and migration of neural stem cells in vitro.Moreover,our data provides novel insights on cell type-specific-dependent requirements of transforming growth factor-βsignaling on neural stem cell proliferation and migration in vivo.
基金Project supported by the National Natural Science Foundation ofChina (No. 60472100), the Natural Science Foundation of ZhejiangProvince (Nos. RC01057, Y105577, 601017), the Ningbo Scienceand Technology Project (Nos. 2003A61001, 2004A610001,2004A630002), and the Zhejiang Science and Technology Project(No. 2004C31105), China
文摘In this work, a new method to deal with the unconnected pixels in motion compensated temporal filtering (MCTF) is presented, which is designed to improve the performance of 3D lifted wavelet coding. Furthermore, multiple description scalable coding (MDSC) is investigated, and novel MDSC schemes based on 3D wavelet coding are proposed, using the lifting imple- mentation of temporal filtering. The proposed MDSC schemes can avoid the mismatch problem in multiple description video coding, and have high scalability and robustness of video transmission. Experimental results showed that the proposed schemes are feasible and adequately effective.
基金Supported by State Administration of Traditional Chinese Medicine Science and Technology Research Projects(Chinese pharmaceutical 2016ZX05)Effect of Wulong Xiaozheng Wan on Intermediate Gastric Carcinoma and its Mechanism
文摘OBJECTIVE:To evaluate the effect of Wulong Xiaozheng Wan medicated serum on the epithelial-mesenchymal transition (EMT) of BGC823 cell induced by transforming growth factor-β,(TGF-β,) and to explore its mechanism.METHODS:EMT model of BGC823 was stimulated by TGF-β1.Wulong Xiaozheng Wan medicated serum and LY-364947 were used as intervention.The proliferation and adhesion of BGC823 were detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide and flow cytometry was used to detect the apoptosis.The invasion and migration were detected by Transwell.The level of matrix metalloproteins was detected by enzyme-linked immunosorbent assay.The expressions of related proteins and mRNA of EMT marker and TGF-β1/Smad signal pathway were detected by Western blot and reverse transcription-polymerase chain reaction.RESULTS:Compared with the TGF-β1 group,Wulong Xiaozheng Wan medicated serum could inhibit the ability of proliferation,heterogeneous adhesion,invasion,and migration.It also promotes apoptosis and homotypic adhesion in BGC823,with a dose-dependent manner.Meanwhile,Wulong Xiaozheng Wan medicated serum could regulate the expression of related proteins and mRNA of TGF-β1/Smad signaling pathway,and inhibit the expressions of EMT transcription factors and EMT markers.CONCLUSION:Wulong Xiaozheng Wan medicated serum inhibited epithelial-mesenchymal transition by down-regulated the expression of TβRI and the activation of TGF-β1/Smad signaling pathway.
文摘In this brief communication we present a new integral transform, so far unknown, which is applicable, for instance, to studying the kinetic theory of natural eigenmodes or transport excited in plasmas with bounded distribution functions such as in Q machines/plasma diodes or in the scrap-off layer of Tokamak fusion plasmas. The results are valid for functions of function spaces—Lebesgue spaces, which are defined using a natural generalization of the p-norm for finite-dimensional vector spaces, where is the real set, σs is the σ-algebra of Lebesgue measurable sets, and the μ Lebesgue measure. , so that . Note that, using a simpler notation, more natural/known to engineers, f could be considered any piecewise continuous function, that is: Here is a Euclidian space with the usual norm (inner product: ) given by: [1].
基金the Deanship of Graduate Studies and Scientific Research at Najran University,Saudi Arabia,for their financial support through the Easy Track Research program,grant code(NU/EFP/MRC/13).
文摘Background:Accurate classification of normal blood cells is a critical foundation for automated hematological analysis,including the detection of pathological conditions like leukemia.While convolutional neural networks(CNNs)excel in local feature extraction,their ability to capture global contextual relationships in complex cellular morphologies is limited.This study introduces a hybrid CNN-Transformer framework to enhance normal blood cell classification,laying the groundwork for future leukemia diagnostics.Methods:The proposed architecture integrates pre-trained CNNs(ResNet50,EfficientNetB3,InceptionV3,CustomCNN)with Vision Transformer(ViT)layers to combine local and global feature modeling.Four hybrid models were evaluated on the publicly available Blood Cell Images dataset from Kaggle,comprising 17,092 annotated normal blood cell images across eight classes.The models were trained using transfer learning,fine-tuning,and computational optimizations,including cross-model parameter sharing to reduce redundancy by reusing weights across CNN backbones and attention-guided layer pruning to eliminate low-contribution layers based on attention scores,improving efficiency without sacrificing accuracy.Results:The InceptionV3-ViT model achieved a weighted accuracy of 97.66%(accounting for class imbalance by weighting each class’s contribution),a macro F1-score of 0.98,and a ROC-AUC of 0.998.The framework excelled in distinguishing morphologically similar cell types demonstrating robustness and reliable calibration(ECE of 0.019).The framework addresses generalization challenges,including class imbalance and morphological similarities,ensuring robust performance across diverse cell types.Conclusion:The hybrid CNN-Transformer framework significantly improves normal blood cell classification by capturing multi-scale features and long-range dependencies.Its high accuracy,efficiency,and generalization position it as a strong baseline for automated hematological analysis,with potential for extension to leukemia subtype classification through future validation on pathological samples.
文摘An effective near-field - far-field (NF - FF) transformation with spherical scanning for quasi-planar antennas from irregularly spaced data is developed in this paper. Two efficient approaches for evaluating the regularly spaced spherical samples from the nonuniformly distributed ones are proposed and numerically compared. Both the approaches rely on a nonredundant sampling representation of the voltage measured by the probe, based on an oblate ellipsoidal modelling of the antenna under test. The former employs the singular value decomposition method to reconstruct the NF data at the points fixed by the nonredundant sampling representation and can be applied when the irregularly acquired samples lie on nonuniform parallels. The latter is based on an iterative technique and can be used also when such a hypothesis does not hold, but requires the existence of a biunique correspondence between the uniform and nonuniform samples, associ- ating at each uniform sampling point the nearest irregular one. Once the regularly spaced spherical samples have been recovered, the NF data needed by a probe compensated NF - FF transformation with spherical scanning are efficiently evaluated by using an optimal sampling interpolation algorithm. It is so possible to accurately compensate known posi- tioning errors in the NF - FF transformation with spherical scanning for quasi-planar antennas. Some numerical tests assessing the accuracy and the robustness of the proposed approaches are reported.
