Powdered activated carbon（PAC） adsorption of two fishy odorants, trans,trans-2,4-heptadienal（HDE） and trans,trans-2,4-decadienal（DDE）, was investigated. Both the pseudo first-order and the pseudo second-order ki...Powdered activated carbon（PAC） adsorption of two fishy odorants, trans,trans-2,4-heptadienal（HDE） and trans,trans-2,4-decadienal（DDE）, was investigated. Both the pseudo first-order and the pseudo second-order kinetic models well described the kinetics curves, and DDE was more readily removed by PAC. In isotherm tests, both Freundlich and Modified Freundlich isotherms fitted the experimental data well. PAC exhibited a higher adsorption capacity for DDE than for HDE, which could be ascribed to the difference in their hydrophobicity. The calculated thermodynamic parameters（ΔG^0, ΔH^0, and ΔS^0） indicated an exothermic and spontaneous adsorption process. PAC dosage, p H, and natural organic matter（NOM） presence were found to influence the adsorption process. With increasing PAC dosage, the pseudo first-order and pseudo second-order rate constants both increased. The value of p H had little influence on HDE or DDE molecules but altered the surface charge of PAC, and the maximum adsorption capacity occurred at p H 9. The presence of NOM, especially the fraction with molecular weight less than1 k Dalton, hindered the adsorption. The study showed that preloaded NOM impaired the adsorption capacity of HDE or DDE more severely than simultaneously fed NOM did.展开更多
The hydration of olefins is one of the most im-portant processes for the production of alcohols[1].Ingeneral,the preparation methods using chiral organo-metallic complexes[2],microorganism[3]or cation-ex-changed montm...The hydration of olefins is one of the most im-portant processes for the production of alcohols[1].Ingeneral,the preparation methods using chiral organo-metallic complexes[2],microorganism[3]or cation-ex-changed montmorillonite[4]suffer from the drawbacks such as poor selectivity,separation difficulty or insta-bility.Enzymes as natural polymeric catalysts have attracted much attention in recent years due to their high activity and selectivity under mild condi-tions[5.6].In imitating the function of an enzyme,the use of natural biopolymers may be one of the ef-fective methods[7].Some natural biopolymer-metal complexes have been prepared and used as the catalyst for hydration to overcome the above disadvan-tages[8,9].Here we report the first results on the use of a wool-Pd complex for the asymmetric hydration of trans-2-butenoic acid.展开更多
Polycystic ovarian syndrome(PCOS)is the most principal reason for infertility in reproductive women,but no versatile treatment is feasible.Although trans-10-hydroxy-2-decenoic acid(10H2DA)exhibits the anti-inflammator...Polycystic ovarian syndrome(PCOS)is the most principal reason for infertility in reproductive women,but no versatile treatment is feasible.Although trans-10-hydroxy-2-decenoic acid(10H2DA)exhibits the anti-inflammatory,anti-cancerous and immunomodulatory properties,its role in improving PCOS remains elusive.This present study examined the effects of 10H2DA on the estrous cycle,ovarian morphology,reproductive hormone,glucose and insulin tolerances of PCOS mice,analyzed the binding between 10H2DA and free fatty acid receptor 4(FFAR4),and explored the regulation of 10H2DA on the c-Jun N-terminal kinase(JNK)/JUN(Jun proto-oncogene)signaling,cytosolic calcium ion(Ca^(2+)),endoplasmic reticulum(ER)Ca^(2+) release,mitochondrial Ca^(2+),mitochondrial function,autophagy,lysosomal acidification,mitochondrial fission,secretion function and apoptosis of ovarian granulosa cells(GCs)using enzyme-linked immunosorbent assay,flow cytometry,Western blotting,molecular docking,various fluorescent probes or plasmid,etc.The results showed that 10H2DA alleviated the symptom of PCOS mice through improving the secretion dysfunction of GCs and protecting GCs against apoptosis.After binding to FFAR4 and inactivation of JNK/JUN signaling,10H2DA restricted the release of ER Ca^(2+)through targeting ryanodine receptors(RYRs)which had been identified as the downstream targets of JUN,diminished cytosolic Ca^(2+) accumulation and prevented Ca^(2+) influx into mitochondria via adjusting the mitochondrial calcium uptake 1 and mitochondrial calcium uniporter.Concurrently,10H2DA kept the integrity of mitochondrial function through maintaining mitochondrial Ca2 homeostasis and suppressed the discharge of mitochondrial reactive oxygen species into the cytosol through blocking the mitochondrial permeability transition pore opening to avoid the lipid peroxidation and ameliorate the secretory function and apoptosis of GCs.Furthermore,1OH2DA rescued the defective autophagic flux along with the decline of autophagosomes and sequestosome 1 aggregation,restored the mitophagy flux with the abatement of mitophagosomes and Parkin recruitment to mitochondria,and accelerated the lysosomal degradation for depolarized mitochondria with the recovery of lysosomal acidification,whereas activation of JNK antagonized the amelioration of 10H2DA on above effectiveness,but this antagonism was counteracted by the attenuation of intracellular Ca^(2+).After application of 10H2DA,dynamin-related protein 1 phosphorylation was diminished and its recruitment to mitochondrial surface was impeded concomitant with the improvement of mitochondrial fragmentation,whereas inhibition of late-stage autophagy caused the failure of 10H2DA in improving mitochondrial fission.Collectively,10H2DA might ameliorate PCOS mice through modulating autophagy-mediated mitochondrial fission dependent on the maintenance of Ca^(2+) homeostasis between ER and mitochondria.展开更多
The title complex [AgNO3(C17H15NO2)2]2 was synthesized and characterized by X-ray diffraction analysis. The compound crystallizes in triclinic, space group P with a = 11.226(7), b = 11.906(7), c = 12.144(7) , = 99.79...The title complex [AgNO3(C17H15NO2)2]2 was synthesized and characterized by X-ray diffraction analysis. The compound crystallizes in triclinic, space group P with a = 11.226(7), b = 11.906(7), c = 12.144(7) , = 99.796(10), = 91.631(10), = 101.225(10), V = 1565.6(16) 3, Z = 1, Mr = 1400.96, Dc = 1.486 g/cm3, F(000) = 716 and (Mo-K? = 0.697 mm-1. The final R and wR are 0.0401 and 0.0995, respectively for 5492 independent observable reflections with I > 2(I). The results show that the central Ag atom is four-coordinated with two O atoms from two distinct NO3- anions and two N atoms from two trans-5-methyl-2-[2-(4-methoxylphenyl)ethenyl]- benzoxazole ligands, and the two Ag atoms bridged with two O atoms give an interesting Ag-O-Ag-O parallelogram structure. The coordination sphere of Ag atom exhibits a heavily distorted tetrahedral geometry and the coordination angles lie in the range of 66.73(12)～129.59(10). The intra- molecular dihedral angles between the benzoxazolyl and phenyl planes are 4.1(3) and 2.9(3), respectively.展开更多
Trans-4-hydroxy-2-hexenal(4-HHE) and trans-4-hydroxy-2-nonenal(4-HNE) are secondary lipid peroxidation products in edible oils, which are cytotoxic and genotoxic. They could covalently bind with protein, phospholipids...Trans-4-hydroxy-2-hexenal(4-HHE) and trans-4-hydroxy-2-nonenal(4-HNE) are secondary lipid peroxidation products in edible oils, which are cytotoxic and genotoxic. They could covalently bind with protein, phospholipids and DNA, further disrupting the normal function of liver, lung and brain.Derivation process was generally conducted during pretreatment before detection and quantification of 4-HHE and 4-HNE. However, the derivation procedures were time consuming and chemical degradation may occur during the process. Hence, this paper aims to establish a simple solid phase extractionhigh performance liquid chromatography(SPE-HPLC) method to determine the 4-HHE and 4-HNE contents in thermally treated soybean oil. C18 solid phase extraction was applied in the pretreatment process. Firstly, the reliability of the method was evaluated. Good linearity was observed in the range of 0.1–0.5 μg/m L and 0.5–10 μg/m L for 4-HHE and 4-HNE. The limit of detection(LOD) of 4-HHE and 4-HNE were 0.0486 and 0.0129 μg/m L, respectively. And the limit of quantitation(LOQ) of4-HHE and 4-HNE were 0.1458 and 0.0431 μg/m L, respectively. Recovery rate were in the range of89.11%–91.58% and 71.83%–79.40% for 4-HHE and 4-HNE, respectively. The method achieved the extraction, purification and detection of 4-HHE and 4-HNE simultaneously and had the advantages of simple operation, effectiveness, high precision, good repeatability. Then, the method was applied to monitor the concentrations of 4-HHE and 4-HNE in soybean oil heated at 180 °C for 40 h. The contents of 4-HHE and 4-HNE were 0–0.32 μg/g and 0–6.97 μg/g, respectively, which provided guidance for evaluating health risks of thermally treated soybean oil during heating.展开更多
The monofunctional substitution reactions between trans-[PtCl（H2O）（NH3）（pip）]＋,trans-[Pt（H2O）2（NH3）（pip）]2＋,trans-[PtCl（H2O）（pip）2]＋,trans-[Pt（H2O）2（pip）2]2＋ （pip = piperidine） and adenine/gu...The monofunctional substitution reactions between trans-[PtCl（H2O）（NH3）（pip）]＋,trans-[Pt（H2O）2（NH3）（pip）]2＋,trans-[PtCl（H2O）（pip）2]＋,trans-[Pt（H2O）2（pip）2]2＋ （pip = piperidine） and adenine/guanine nucleotides are explored by using B3LYP hybrid functional and IEF-PCM salvation models. For the trans-[Pt（H2O）2（NH3）（pip）]2＋ and trans-[PtCl（H2O）（NH3）（pip）]＋ complexes,the computed barrier heights in aqueous solution are 13.5/13.5 and 11.6/11.6 kcal/mol from trans-Pt-chloroaqua complex to trans/cis-monoadduct for adenine and guanine,and the corresponding values are 20.7/20.7 and 18.8/18.8 kcal/mol from trans-Pt-diaqua complex to trans/cis-monoadduct for adenine and guanine,respectively. For trans-[PtCl（H2O）（pip）2]＋ and trans-[Pt（H2O）2（pip）2]2＋,the corresponding values are 21.5/21.3 and 19.4/19.4 kcal/mol,and 26.0/26.0 and 20.7/20.8 kal/mol for adenine and guanine,respectively. Our calculations demonstrate that the barrier heights of chloroaqua are lower than the corresponding values of diaqua for adenine and guanine. In addition,the free energies of activation for guanine in aqueous solution are all smaller than that for adenine,which predicts a preference of 1.9 kcal/mol when trans-[PtCl（H2O）（NH3）（pip）]＋ and trans-[Pt（H2O）2（NH3）（pip）]2＋ are the active agents and ～1.9 and ～ 5.3 kcal/mol when trans-[PtCl（H2O）（pip）2]＋ and trans-[Pt（H2O）2（pip）2]2＋ are the active agents,respectively. For the reaction of trans-Pt-chloroaqua （or diaqua） to cis-monoadduct,we obtain the same transition-state structure as from the reaction of trans-Pt-chloroaqua （or diaqua） to trans-monoadduct,which seems that the trans-Pt-chloroaqua （or diaqua） complex can generate trans-or cis-monoadduct via the same transition-state.展开更多
The title compound of trans-3-phenyl-2-propenoic acid （C9H802）, a main active organic acid of cinnamon bark and styrax, was obtained by oxidization from trans-3-phenyl-2-propenaldehyde and characterized by X-ray dif...The title compound of trans-3-phenyl-2-propenoic acid （C9H802）, a main active organic acid of cinnamon bark and styrax, was obtained by oxidization from trans-3-phenyl-2-propenaldehyde and characterized by X-ray diffraction analysis. It crystallizes in monoclinic system, space group P21/n with a = 5.55916（10）, b = 17.4870（3）, c = 7.70677（13） A, V = 744.89（2） A3, Z= 4, Dc= 1.321 g/cm3, Mr = 148.15, F（000） = 312, and/t μ=0.764 mm-1. A total of 2661 reflections with 1447 unique ones （Rint = 0.0137） were collected, in which 1447 were observed （I〉2σ（/））. There were two C9H802 molecules in an asymmetric unit. The structural unit was a dimer formed by the hydrogen bonding of the oxygen atom of carbonyl group and the hydrogen atom of hydroxyl group. A 3D layer structure was formed through hydrogen bonds and Van der Walls＇ forces.展开更多
Pea aphid,Acyrthosiphon pisum,is a serious pest of many different leguminous plants,and it mainly relies on its odorant receptors(Ors)to discriminate among host species.However,less is known about the role that Ors pl...Pea aphid,Acyrthosiphon pisum,is a serious pest of many different leguminous plants,and it mainly relies on its odorant receptors(Ors)to discriminate among host species.However,less is known about the role that Ors play in the host plant location.In this study,we identified a novel conserved odorant receptor clade by phylogenetic analysis,and conducted the functional analysis of ApisOr23 in A.pisum.The results showed that the homologous Ors from A.pisum,Aphis glycines and Aphis gossypii share 94.28% identity in amino acid sequences.Moreover,conserved motifs were analyzed using the annotated homologous Or23 from eight aphid species,providing further proof of the high conservation level of the Or23 clade.According to the tissue expression pattern analysis,ApisOr23 was mainly expressed in the antennae.Further functional study using a heterologous Xenopus expression system revealed that ApisOr23 was tuned to five plant volatiles,namely trans-2-hexen-1-al,cis-2-hexen-1-ol,1-heptanol,4’-ethylacetophenone,and hexyl acetate.Among them,trans-2-hexen-1-al,which is one of the main volatile organic compounds released from legume plants,activated the highest response of ApisOr23.Our findings suggest that the conserved Or23 clade in most aphid species might play an important role in host plant detection.展开更多
A square wave voltammetry (DPV) method for trans-Pt[Cl2(Dimethylamine)(isopropylamine)] determination is developed. To this end, all the chemical and instrumental variables affecting the determination of trans-Pt[Cl2(...A square wave voltammetry (DPV) method for trans-Pt[Cl2(Dimethylamine)(isopropylamine)] determination is developed. To this end, all the chemical and instrumental variables affecting the determination of trans-Pt[Cl2(Dimethylamine) (isopropylamine)] are optimized. From studies of the mechanisms governing the electrochemical response of trans-Pt[Cl2(Dimethylamine)(isopropylamine)], it was concluded that it was an electrochemically reversible system with an adsorptive oxidation phenomenon. Under optimal conditions, the variation of analytical signal (Ip) with trans-Pt[Cl2(Dimethylamine)(isopropylamine)] concentration was linear in the 0.05 μg·mL-1 to 10 μg·mL-1 range, with a LOD 91 μg·mL-1 of and a LOQ of 303 μg·mL-1, a RSD 1.10% and Er 0.72%. The optimized method was applied to the determination of trans-Pt[Cl2(Dimethylamine)(isopropylamine)] in biological fluids, in human urine and synthetic urine.展开更多
The crystal and molecular structures of cydta (trans-1, 2-cyclohexanediaminetetraacetic acid) have been determined by single\|crystal X-ray structure analyses. The reasons that RE Ⅲ -edta and RE Ⅲ -cydta compl...The crystal and molecular structures of cydta (trans-1, 2-cyclohexanediaminetetraacetic acid) have been determined by single\|crystal X-ray structure analyses. The reasons that RE Ⅲ -edta and RE Ⅲ -cydta complexes select different coordinate structures were discussed and illustrated through the comparison of the molecular and crystal structures between edta and cydta, (NH_ 4 )\[Y Ⅲ (edta)(H_ 2 O)_ 3 \]\55H_ 2 O and (NH_ 4 )\[Y Ⅲ (cydta)(H_ 2 O)_ 2 \]\55H_ 2 O as well as (NH_ 4 )\[Gd Ⅲ (edta)(H_ 2 O)_ 3 \]\55H_ 2 O and (NH_ 4 )\[Gd Ⅲ (cydta) (H_ 2 O) _ 2 \]\55H_ 2 O complexes.展开更多
The interaction of several anti-tumor metal complexes with dGMP have been investigated using trans-[en_(2)Os(h^(2)-H_(2))]^(2+)as a ^(1)H NMR probe in a competitive mode.Me_(2)SnCl_(2),Bu_(2)SnCl_(2),Et_(2)Sn(phen)Cl_...The interaction of several anti-tumor metal complexes with dGMP have been investigated using trans-[en_(2)Os(h^(2)-H_(2))]^(2+)as a ^(1)H NMR probe in a competitive mode.Me_(2)SnCl_(2),Bu_(2)SnCl_(2),Et_(2)Sn(phen)Cl_(2) and Et_(2)SnCl_(2) can bind to dGMP mainly via phosphate;Cp_(2)TiCl_(2) binds to dGMP mainly via phosphate and N_(7).The binding constant for(CH_(3))_(2)SnCl_(2) binding to phos-phate of dGMP exceeds 2.71×10^(4).The binding constant for Cp_(2)TiCl_(2) to phosphate is even greater than that of Sn(IV).Cis-platin has high affinity for both N_(7) and phosphate,but mainly for N_(7).Binding of the probe to N_(7) of dGMP reduces the binding affinity for phosphate of the same dGMP molecule by a factor of 5 to 6.Much the same factor is expected to apply to other metals containing agents interacting with dGMP.展开更多
基金supported by the National Natural Science Foundation of China (No. 51290284)
文摘Powdered activated carbon（PAC） adsorption of two fishy odorants, trans,trans-2,4-heptadienal（HDE） and trans,trans-2,4-decadienal（DDE）, was investigated. Both the pseudo first-order and the pseudo second-order kinetic models well described the kinetics curves, and DDE was more readily removed by PAC. In isotherm tests, both Freundlich and Modified Freundlich isotherms fitted the experimental data well. PAC exhibited a higher adsorption capacity for DDE than for HDE, which could be ascribed to the difference in their hydrophobicity. The calculated thermodynamic parameters（ΔG^0, ΔH^0, and ΔS^0） indicated an exothermic and spontaneous adsorption process. PAC dosage, p H, and natural organic matter（NOM） presence were found to influence the adsorption process. With increasing PAC dosage, the pseudo first-order and pseudo second-order rate constants both increased. The value of p H had little influence on HDE or DDE molecules but altered the surface charge of PAC, and the maximum adsorption capacity occurred at p H 9. The presence of NOM, especially the fraction with molecular weight less than1 k Dalton, hindered the adsorption. The study showed that preloaded NOM impaired the adsorption capacity of HDE or DDE more severely than simultaneously fed NOM did.
基金Supported by the Scholarship Foundation of Shanxi Province and the Natural Science Foundation of Shanxi Province(021109).
文摘The hydration of olefins is one of the most im-portant processes for the production of alcohols[1].Ingeneral,the preparation methods using chiral organo-metallic complexes[2],microorganism[3]or cation-ex-changed montmorillonite[4]suffer from the drawbacks such as poor selectivity,separation difficulty or insta-bility.Enzymes as natural polymeric catalysts have attracted much attention in recent years due to their high activity and selectivity under mild condi-tions[5.6].In imitating the function of an enzyme,the use of natural biopolymers may be one of the ef-fective methods[7].Some natural biopolymer-metal complexes have been prepared and used as the catalyst for hydration to overcome the above disadvan-tages[8,9].Here we report the first results on the use of a wool-Pd complex for the asymmetric hydration of trans-2-butenoic acid.
基金supported by Natural Science Foundation of Jilin Province(20220101287JC)National Natural Science Foundation of China(31873003 and 31472158)Graduate Innovation Fund of Jilin University(2022140).
文摘Polycystic ovarian syndrome(PCOS)is the most principal reason for infertility in reproductive women,but no versatile treatment is feasible.Although trans-10-hydroxy-2-decenoic acid(10H2DA)exhibits the anti-inflammatory,anti-cancerous and immunomodulatory properties,its role in improving PCOS remains elusive.This present study examined the effects of 10H2DA on the estrous cycle,ovarian morphology,reproductive hormone,glucose and insulin tolerances of PCOS mice,analyzed the binding between 10H2DA and free fatty acid receptor 4(FFAR4),and explored the regulation of 10H2DA on the c-Jun N-terminal kinase(JNK)/JUN(Jun proto-oncogene)signaling,cytosolic calcium ion(Ca^(2+)),endoplasmic reticulum(ER)Ca^(2+) release,mitochondrial Ca^(2+),mitochondrial function,autophagy,lysosomal acidification,mitochondrial fission,secretion function and apoptosis of ovarian granulosa cells(GCs)using enzyme-linked immunosorbent assay,flow cytometry,Western blotting,molecular docking,various fluorescent probes or plasmid,etc.The results showed that 10H2DA alleviated the symptom of PCOS mice through improving the secretion dysfunction of GCs and protecting GCs against apoptosis.After binding to FFAR4 and inactivation of JNK/JUN signaling,10H2DA restricted the release of ER Ca^(2+)through targeting ryanodine receptors(RYRs)which had been identified as the downstream targets of JUN,diminished cytosolic Ca^(2+) accumulation and prevented Ca^(2+) influx into mitochondria via adjusting the mitochondrial calcium uptake 1 and mitochondrial calcium uniporter.Concurrently,10H2DA kept the integrity of mitochondrial function through maintaining mitochondrial Ca2 homeostasis and suppressed the discharge of mitochondrial reactive oxygen species into the cytosol through blocking the mitochondrial permeability transition pore opening to avoid the lipid peroxidation and ameliorate the secretory function and apoptosis of GCs.Furthermore,1OH2DA rescued the defective autophagic flux along with the decline of autophagosomes and sequestosome 1 aggregation,restored the mitophagy flux with the abatement of mitophagosomes and Parkin recruitment to mitochondria,and accelerated the lysosomal degradation for depolarized mitochondria with the recovery of lysosomal acidification,whereas activation of JNK antagonized the amelioration of 10H2DA on above effectiveness,but this antagonism was counteracted by the attenuation of intracellular Ca^(2+).After application of 10H2DA,dynamin-related protein 1 phosphorylation was diminished and its recruitment to mitochondrial surface was impeded concomitant with the improvement of mitochondrial fragmentation,whereas inhibition of late-stage autophagy caused the failure of 10H2DA in improving mitochondrial fission.Collectively,10H2DA might ameliorate PCOS mice through modulating autophagy-mediated mitochondrial fission dependent on the maintenance of Ca^(2+) homeostasis between ER and mitochondria.
基金This project was supported by the Foundation of University Key Teacher by the Ministry of Education
文摘The title complex [AgNO3(C17H15NO2)2]2 was synthesized and characterized by X-ray diffraction analysis. The compound crystallizes in triclinic, space group P with a = 11.226(7), b = 11.906(7), c = 12.144(7) , = 99.796(10), = 91.631(10), = 101.225(10), V = 1565.6(16) 3, Z = 1, Mr = 1400.96, Dc = 1.486 g/cm3, F(000) = 716 and (Mo-K? = 0.697 mm-1. The final R and wR are 0.0401 and 0.0995, respectively for 5492 independent observable reflections with I > 2(I). The results show that the central Ag atom is four-coordinated with two O atoms from two distinct NO3- anions and two N atoms from two trans-5-methyl-2-[2-(4-methoxylphenyl)ethenyl]- benzoxazole ligands, and the two Ag atoms bridged with two O atoms give an interesting Ag-O-Ag-O parallelogram structure. The coordination sphere of Ag atom exhibits a heavily distorted tetrahedral geometry and the coordination angles lie in the range of 66.73(12)～129.59(10). The intra- molecular dihedral angles between the benzoxazolyl and phenyl planes are 4.1(3) and 2.9(3), respectively.
基金supported by grants from the National Natural Science Foundation of China(No.31471668)。
文摘Trans-4-hydroxy-2-hexenal(4-HHE) and trans-4-hydroxy-2-nonenal(4-HNE) are secondary lipid peroxidation products in edible oils, which are cytotoxic and genotoxic. They could covalently bind with protein, phospholipids and DNA, further disrupting the normal function of liver, lung and brain.Derivation process was generally conducted during pretreatment before detection and quantification of 4-HHE and 4-HNE. However, the derivation procedures were time consuming and chemical degradation may occur during the process. Hence, this paper aims to establish a simple solid phase extractionhigh performance liquid chromatography(SPE-HPLC) method to determine the 4-HHE and 4-HNE contents in thermally treated soybean oil. C18 solid phase extraction was applied in the pretreatment process. Firstly, the reliability of the method was evaluated. Good linearity was observed in the range of 0.1–0.5 μg/m L and 0.5–10 μg/m L for 4-HHE and 4-HNE. The limit of detection(LOD) of 4-HHE and 4-HNE were 0.0486 and 0.0129 μg/m L, respectively. And the limit of quantitation(LOQ) of4-HHE and 4-HNE were 0.1458 and 0.0431 μg/m L, respectively. Recovery rate were in the range of89.11%–91.58% and 71.83%–79.40% for 4-HHE and 4-HNE, respectively. The method achieved the extraction, purification and detection of 4-HHE and 4-HNE simultaneously and had the advantages of simple operation, effectiveness, high precision, good repeatability. Then, the method was applied to monitor the concentrations of 4-HHE and 4-HNE in soybean oil heated at 180 °C for 40 h. The contents of 4-HHE and 4-HNE were 0–0.32 μg/g and 0–6.97 μg/g, respectively, which provided guidance for evaluating health risks of thermally treated soybean oil during heating.
基金supported from the National Natural Science Foundation of China (No. 20971056)
文摘The monofunctional substitution reactions between trans-[PtCl（H2O）（NH3）（pip）]＋,trans-[Pt（H2O）2（NH3）（pip）]2＋,trans-[PtCl（H2O）（pip）2]＋,trans-[Pt（H2O）2（pip）2]2＋ （pip = piperidine） and adenine/guanine nucleotides are explored by using B3LYP hybrid functional and IEF-PCM salvation models. For the trans-[Pt（H2O）2（NH3）（pip）]2＋ and trans-[PtCl（H2O）（NH3）（pip）]＋ complexes,the computed barrier heights in aqueous solution are 13.5/13.5 and 11.6/11.6 kcal/mol from trans-Pt-chloroaqua complex to trans/cis-monoadduct for adenine and guanine,and the corresponding values are 20.7/20.7 and 18.8/18.8 kcal/mol from trans-Pt-diaqua complex to trans/cis-monoadduct for adenine and guanine,respectively. For trans-[PtCl（H2O）（pip）2]＋ and trans-[Pt（H2O）2（pip）2]2＋,the corresponding values are 21.5/21.3 and 19.4/19.4 kcal/mol,and 26.0/26.0 and 20.7/20.8 kal/mol for adenine and guanine,respectively. Our calculations demonstrate that the barrier heights of chloroaqua are lower than the corresponding values of diaqua for adenine and guanine. In addition,the free energies of activation for guanine in aqueous solution are all smaller than that for adenine,which predicts a preference of 1.9 kcal/mol when trans-[PtCl（H2O）（NH3）（pip）]＋ and trans-[Pt（H2O）2（NH3）（pip）]2＋ are the active agents and ～1.9 and ～ 5.3 kcal/mol when trans-[PtCl（H2O）（pip）2]＋ and trans-[Pt（H2O）2（pip）2]2＋ are the active agents,respectively. For the reaction of trans-Pt-chloroaqua （or diaqua） to cis-monoadduct,we obtain the same transition-state structure as from the reaction of trans-Pt-chloroaqua （or diaqua） to trans-monoadduct,which seems that the trans-Pt-chloroaqua （or diaqua） complex can generate trans-or cis-monoadduct via the same transition-state.
基金The China Agriculture Research System(Grant No.CARS-21)the public welfare research special project in General Administration of Quality Supervision,Inspection and Quarantine of the People’s Republic of China(Grant No.201210209)
文摘The title compound of trans-3-phenyl-2-propenoic acid （C9H802）, a main active organic acid of cinnamon bark and styrax, was obtained by oxidization from trans-3-phenyl-2-propenaldehyde and characterized by X-ray diffraction analysis. It crystallizes in monoclinic system, space group P21/n with a = 5.55916（10）, b = 17.4870（3）, c = 7.70677（13） A, V = 744.89（2） A3, Z= 4, Dc= 1.321 g/cm3, Mr = 148.15, F（000） = 312, and/t μ=0.764 mm-1. A total of 2661 reflections with 1447 unique ones （Rint = 0.0137） were collected, in which 1447 were observed （I〉2σ（/））. There were two C9H802 molecules in an asymmetric unit. The structural unit was a dimer formed by the hydrogen bonding of the oxygen atom of carbonyl group and the hydrogen atom of hydroxyl group. A 3D layer structure was formed through hydrogen bonds and Van der Walls＇ forces.
基金funded by the National Natural Science Foundation of China(31572072 and 31725023)the Intergovernmental International Science,Technology and Innovation Cooperation Key Project,China(2019YFE0105800)the Shenzhen Science and Technology Program,China(KQTD20180411143628272)。
文摘Pea aphid,Acyrthosiphon pisum,is a serious pest of many different leguminous plants,and it mainly relies on its odorant receptors(Ors)to discriminate among host species.However,less is known about the role that Ors play in the host plant location.In this study,we identified a novel conserved odorant receptor clade by phylogenetic analysis,and conducted the functional analysis of ApisOr23 in A.pisum.The results showed that the homologous Ors from A.pisum,Aphis glycines and Aphis gossypii share 94.28% identity in amino acid sequences.Moreover,conserved motifs were analyzed using the annotated homologous Or23 from eight aphid species,providing further proof of the high conservation level of the Or23 clade.According to the tissue expression pattern analysis,ApisOr23 was mainly expressed in the antennae.Further functional study using a heterologous Xenopus expression system revealed that ApisOr23 was tuned to five plant volatiles,namely trans-2-hexen-1-al,cis-2-hexen-1-ol,1-heptanol,4’-ethylacetophenone,and hexyl acetate.Among them,trans-2-hexen-1-al,which is one of the main volatile organic compounds released from legume plants,activated the highest response of ApisOr23.Our findings suggest that the conserved Or23 clade in most aphid species might play an important role in host plant detection.
文摘A square wave voltammetry (DPV) method for trans-Pt[Cl2(Dimethylamine)(isopropylamine)] determination is developed. To this end, all the chemical and instrumental variables affecting the determination of trans-Pt[Cl2(Dimethylamine) (isopropylamine)] are optimized. From studies of the mechanisms governing the electrochemical response of trans-Pt[Cl2(Dimethylamine)(isopropylamine)], it was concluded that it was an electrochemically reversible system with an adsorptive oxidation phenomenon. Under optimal conditions, the variation of analytical signal (Ip) with trans-Pt[Cl2(Dimethylamine)(isopropylamine)] concentration was linear in the 0.05 μg·mL-1 to 10 μg·mL-1 range, with a LOD 91 μg·mL-1 of and a LOQ of 303 μg·mL-1, a RSD 1.10% and Er 0.72%. The optimized method was applied to the determination of trans-Pt[Cl2(Dimethylamine)(isopropylamine)] in biological fluids, in human urine and synthetic urine.
文摘The crystal and molecular structures of cydta (trans-1, 2-cyclohexanediaminetetraacetic acid) have been determined by single\|crystal X-ray structure analyses. The reasons that RE Ⅲ -edta and RE Ⅲ -cydta complexes select different coordinate structures were discussed and illustrated through the comparison of the molecular and crystal structures between edta and cydta, (NH_ 4 )\[Y Ⅲ (edta)(H_ 2 O)_ 3 \]\55H_ 2 O and (NH_ 4 )\[Y Ⅲ (cydta)(H_ 2 O)_ 2 \]\55H_ 2 O as well as (NH_ 4 )\[Gd Ⅲ (edta)(H_ 2 O)_ 3 \]\55H_ 2 O and (NH_ 4 )\[Gd Ⅲ (cydta) (H_ 2 O) _ 2 \]\55H_ 2 O complexes.
基金the National Natural Science Foundation of China(Grant No.20171031)Provincial Natural Foundation of Shanxi.
文摘The interaction of several anti-tumor metal complexes with dGMP have been investigated using trans-[en_(2)Os(h^(2)-H_(2))]^(2+)as a ^(1)H NMR probe in a competitive mode.Me_(2)SnCl_(2),Bu_(2)SnCl_(2),Et_(2)Sn(phen)Cl_(2) and Et_(2)SnCl_(2) can bind to dGMP mainly via phosphate;Cp_(2)TiCl_(2) binds to dGMP mainly via phosphate and N_(7).The binding constant for(CH_(3))_(2)SnCl_(2) binding to phos-phate of dGMP exceeds 2.71×10^(4).The binding constant for Cp_(2)TiCl_(2) to phosphate is even greater than that of Sn(IV).Cis-platin has high affinity for both N_(7) and phosphate,but mainly for N_(7).Binding of the probe to N_(7) of dGMP reduces the binding affinity for phosphate of the same dGMP molecule by a factor of 5 to 6.Much the same factor is expected to apply to other metals containing agents interacting with dGMP.
