OBJECTIVE:This work aims to observe the effects of electroacupuncture on brain-derived neuro-trophic factor(BDNF)mRNA expression in mouse hippocampus following cerebral ischemia-reperfu-sion injury.METHODS:The models ...OBJECTIVE:This work aims to observe the effects of electroacupuncture on brain-derived neuro-trophic factor(BDNF)mRNA expression in mouse hippocampus following cerebral ischemia-reperfu-sion injury.METHODS:The models of mouse cerebral isch-emia-reperfusion injury were established.A total of 96 healthy mice were randomly assigned into 4 groups,namely,the sham surgery,model,mod-el+electroacupuncture,and mode+hydergine groups.Mice in the model+electroacupuncturegroup were treated through electroacupuncture at the Shenshu(BL 23),Geshu(BL 17),and Baihui(GV 20)acupoints.Mice in the model+hydergine group were intragastrically administered with hy-dergine(0.77 mg/kg-1.day-l).The levels of BDNF mRNA expressions in the hippocampus were ana-lyzed through a semi-quantitative reverse transcrip-tion-polymerase chain reaction assay on days 1 and 7 after the surgeries.RESULTS:BDNF mRNA expressions in the mouse hippocampus of the model group on days 1 and 7 after the surgery were higher than those of the sham surgery group(both P〈0.01).On days 1 and 7 of the electroacupuncture treatment,BDNF mRNA expression in the mouse hippocampus of the mod-el+electroacupuncture group was significantly ele-vated compared with the model group(both P〈0.01)or the model+hydergine group(both P〈0.01).On days 1 and 7 of the hydergine treatment,BDNF mRNA expression in the mouse hippocam-pus of the model+hydergine group tended to in-crease compared with the model group;however,statistical significance was not achieved(both P〉0.05).CONCLUSION:Electroacupuncture treatment en-hances endogenous BDNF expression,which may improve the survival environment for intracerebral neurons and inhibit the apoptosis of hippocampal cells.展开更多
基金Supported by Department of Science&Technology of He-bei Province(No.06276102D-31)Department of Health of Hebei Province(No.2005156)Training Program for Backbone of Scientific Research Talents of Hebei Medical University(2007)
文摘OBJECTIVE:This work aims to observe the effects of electroacupuncture on brain-derived neuro-trophic factor(BDNF)mRNA expression in mouse hippocampus following cerebral ischemia-reperfu-sion injury.METHODS:The models of mouse cerebral isch-emia-reperfusion injury were established.A total of 96 healthy mice were randomly assigned into 4 groups,namely,the sham surgery,model,mod-el+electroacupuncture,and mode+hydergine groups.Mice in the model+electroacupuncturegroup were treated through electroacupuncture at the Shenshu(BL 23),Geshu(BL 17),and Baihui(GV 20)acupoints.Mice in the model+hydergine group were intragastrically administered with hy-dergine(0.77 mg/kg-1.day-l).The levels of BDNF mRNA expressions in the hippocampus were ana-lyzed through a semi-quantitative reverse transcrip-tion-polymerase chain reaction assay on days 1 and 7 after the surgeries.RESULTS:BDNF mRNA expressions in the mouse hippocampus of the model group on days 1 and 7 after the surgery were higher than those of the sham surgery group(both P〈0.01).On days 1 and 7 of the electroacupuncture treatment,BDNF mRNA expression in the mouse hippocampus of the mod-el+electroacupuncture group was significantly ele-vated compared with the model group(both P〈0.01)or the model+hydergine group(both P〈0.01).On days 1 and 7 of the hydergine treatment,BDNF mRNA expression in the mouse hippocam-pus of the model+hydergine group tended to in-crease compared with the model group;however,statistical significance was not achieved(both P〉0.05).CONCLUSION:Electroacupuncture treatment en-hances endogenous BDNF expression,which may improve the survival environment for intracerebral neurons and inhibit the apoptosis of hippocampal cells.
