目的探讨不同周龄转基因前列腺癌小鼠模型（transgenic adenocarcinoma of mouse prostate，TRAMP）的病理学特点。方法TRAMP小鼠饲养于SPF级环境中，记录其生存周期；通过组织病理学切片、HE染色及免疫组化法观察不同周龄TRAMP小鼠前...目的探讨不同周龄转基因前列腺癌小鼠模型（transgenic adenocarcinoma of mouse prostate，TRAMP）的病理学特点。方法TRAMP小鼠饲养于SPF级环境中，记录其生存周期；通过组织病理学切片、HE染色及免疫组化法观察不同周龄TRAMP小鼠前列腺的病理学变化、SV40Tantigen（Tag）表达及细胞增殖。结果TRAMP小鼠普遍生存周期为30～55周，随着周龄的增加其前列腺异型增生程度愈加明显，16周左右处于明显增生期，24周左右发生腺瘤，32周左右出现低分化癌，55周时发现明显的前列腺癌神经内分泌分化现象。免疫表型：随着周龄的增加，TRAMP小鼠前列腺上皮细胞Tag的表达增强且增殖明显。结论TRAMP小鼠可稳定自发前列腺癌，出现前列腺上皮内瘤、高分化腺癌、低分化癌和神经内分泌癌的病理学特点，可作为人类前列腺癌研究的经典动物模型。展开更多
The most appropriate time to introduce androgen deprivation therapy for prostate cancer remains controversial. Our aim was to evaluate the effects of early versus delayed surgical castration on prostate cancer progres...The most appropriate time to introduce androgen deprivation therapy for prostate cancer remains controversial. Our aim was to evaluate the effects of early versus delayed surgical castration on prostate cancer progression and survival in the transgenic adenocarcinoma of the mouse prostate （TRAMP） model. TRAMP mice were randomly divided into three groups： the early castration group （on which castration was performed at the age of 4 weeks）, the delayed castration group （on which castration was performed when abdominal tumours could be palpated）, and the sham-castrated group. Mice were monitored daily throughout their lives until cancer-related death or the develop- ment of an obviously moribund appearance, at which time the individual mouse was killed. Androgen receptor expression in prostate tumours was also evaluated. The results shows that the average lifespan in early castration, delayed castration and sham-castrated groups were 54.1 weeks, 59.9 weeks and 39.1 weeks, respectively. Both early castration and delayed castration conferred a statistically significant survival advantage when compared with the sham-castrated group （P 〈 0.001）. However, the difference in lifespan between the early castration group and the delayed castration group was not statistically significant （P = 0.85）. The increase in lifespan in the TRAMP mice that received either early or delayed castration correlated with lower G/B value （genitourinary tract weight/body weight） at death than the sham-castrated mice. In conclusion, early and delayed castrations in TRAMP mice pro- longed survival to a similar extent. This finding may provide a guide for clinical practice in prostate cancer therapy.展开更多
Many invasive invertebrates belong to unresolved species complexes, and have a history of misidentification and in-appropriate management. Here we detail confusion surrounding the taxonomy and identification of two do...Many invasive invertebrates belong to unresolved species complexes, and have a history of misidentification and in-appropriate management. Here we detail confusion surrounding the taxonomy and identification of two dolichoderine ant species, Iridomyrmex anceps Roger and OcheteUus glaber Mayr, which are commonly cited in the literature as having been spread widely by human commerce. We provide morphometric data and images strongly suggesting that these two ＂species＂ are instead com- plexes of species that are likely native to many regions where they are purportedly introduced. We take particular interest in the status of species referred to as L anceps and O. glaber in China, as this is the most northerly distribution of both genera. We con-clude by suggesting that many identifications of both species are likely to be unreliable, and we recommend against any manage- ment action aimed at controlling these species under the assumption that they are exotic [Current Zoology 57 （5）： 662-667, 2011].展开更多
The effect of tramp elements in the steel was intensively studied. It was found that the solubility of tramp elements decreased as the temperature decreased under normal cooling conditions. The tramp elements （Cu, Pb...The effect of tramp elements in the steel was intensively studied. It was found that the solubility of tramp elements decreased as the temperature decreased under normal cooling conditions. The tramp elements （Cu, Pb, and Sn） diffused toward the grain boundaries, and intermetallic compounds or rich phases which have low melting points were formed, causing reduction in ductility and failure during the bending test. Rebars with Cu content which were left to air cooling after the last step showed drop in elongation, up to 32 %. On contrast, the samples with high per- centage of tramp elements （Cu, Pb, and Sn） in the billet, which were rolled and subjected to Tempcore process, did not show drop in elongation or failure in bending test （especially for rebar with diameter less than 32 mm）; however, copper must be less than 0.35 mass% to prevent the precipitation of Cu-rich zones of critical size in 32 mm. When quench- ing was applied, the tramp elements remained in the interstitial supersaturated solid solution positions inside the grains and would not have the chance to diffuse and form precipitates, hindering the copper precipitates from reac- hing the critical size necessary for impairing the properties. This would hinder the occurrence of the harmful effect of the tramp elements on the elongation or the hot shortness after rolling.展开更多
In the thermodynamic evaluation Y was selected as a typical heavy rare earth metal for reducing the detrmental effects of tramp elements in the petroleum pipe steel. The possibilities of reactions between Y and trampe...In the thermodynamic evaluation Y was selected as a typical heavy rare earth metal for reducing the detrmental effects of tramp elements in the petroleum pipe steel. The possibilities of reactions between Y and trampelements such as Sn, Sb, As, etc. after deoxidation and desulphurization were estimated by calculating the Gibbsfree energies of formation. The equilibrium content of Y for eliminating the detrimental effects of tramp elements inthe steel was recommended from the results of thermodynamic calculation.展开更多
Diets rich in soy products may reduce the risk of prostate cancer (PCa). Daidzein, the major isoflavone present in soy germ, can be metabolized by the gut microbiota into equol. The effects of daidzein and equol on PC...Diets rich in soy products may reduce the risk of prostate cancer (PCa). Daidzein, the major isoflavone present in soy germ, can be metabolized by the gut microbiota into equol. The effects of daidzein and equol on PCa have not been well studied. The objective of this study was to investigate the effect of feeding 2% soy germ, 92 ppm daidzein, or 88 ppm equol diets on the progression of PCa in the transgenic adenocarcinoma of the mouse prostate (TRAMP) model. 3-week old male C57BL/6 X FVB TRAMP mice were weaned from our breeding colony and immediately acclimated to an AIN-93G control diet for one week. At 4 weeks of age, mice (n = 30 per diet group) were randomized to one of four pelleted study diets until 18 weeks of age. Unexpectedly, we did not detect any statistical differences in cancer incidence between diets. We suggest that these results are due to the physical attributes of the pelleted diets in the current study. Mice fed pelleted diets had reduced food intake and significantly decreased body weights (p < 0.001) compared to previous studies. A reduction in food intake is known to reduce cancer incidence in a number of cancer models and is likely to have contributed to the decrease in expected cancer incidence in the current study. In conclusion, we suggest that the hardness of the diets pellets could result in a decreased cancer incidence in TRAMP mice.展开更多
Most disturbed habitats in the tropics and subtropics harbor numerous species of invasive ants, and occasionally the same species has been introduced repeatedly from multiple geographical sources. We examined how expe...Most disturbed habitats in the tropics and subtropics harbor numerous species of invasive ants, and occasionally the same species has been introduced repeatedly from multiple geographical sources. We examined how experimental crossbreeding between sexuals from different populations affects the fitness of queens of the tramp ant Cardiocondyla itsukii, which is widely distributed in Asia and the Pacific Islands. Eggs laid by queens that mated with nestmate males had a higher hatchi ng rate than eggs laid by queens mated to males from neighboring (Hawaii x Kauai) or distant introduced populations (Hawaii/Kauai x Okinawa). Furthermore, inbreeding queens had a Ion ger lifespan and produced a less female-biased offspring sex ratio than queens from allopatric mating. This suggests that the genetic divergence between different source populations may already be so large that in case of multiple invasions eventual crossbreeding might negatively affect the fitness of tramp ants.展开更多
Tramp-tolerance characters of C299 of Cynodon dactylon (L.) Pers. and two hybrids ‘Tifdwarf’ and ‘Tifgreen’ of C. dactylon×C. transvaalensis Burtt-Davy were evaluated under the light-tramp, medium-tramp and s...Tramp-tolerance characters of C299 of Cynodon dactylon (L.) Pers. and two hybrids ‘Tifdwarf’ and ‘Tifgreen’ of C. dactylon×C. transvaalensis Burtt-Davy were evaluated under the light-tramp, medium-tramp and serious-tramp with a 75 kg tramper. The results indicated that tramp caused leaf blades of C299 narrower, color lighter and turf quality lower; but the turf height and internode length of C299 with different rolling intensity were not obviously different from that of ‘Tifgreen’. The turf quality of C299 under serious tramp is higher than that of ‘Tifgreen’ and ‘Tifdwarf’. C299 had stronger ability to recover in vertical direction than that of ‘Tifgreen’ and ‘Tifdwarf’ under the tramp. The results show that C299 has the high turf quality and tramp tolerance charater.展开更多
文摘目的探讨不同周龄转基因前列腺癌小鼠模型（transgenic adenocarcinoma of mouse prostate，TRAMP）的病理学特点。方法TRAMP小鼠饲养于SPF级环境中，记录其生存周期；通过组织病理学切片、HE染色及免疫组化法观察不同周龄TRAMP小鼠前列腺的病理学变化、SV40Tantigen（Tag）表达及细胞增殖。结果TRAMP小鼠普遍生存周期为30～55周，随着周龄的增加其前列腺异型增生程度愈加明显，16周左右处于明显增生期，24周左右发生腺瘤，32周左右出现低分化癌，55周时发现明显的前列腺癌神经内分泌分化现象。免疫表型：随着周龄的增加，TRAMP小鼠前列腺上皮细胞Tag的表达增强且增殖明显。结论TRAMP小鼠可稳定自发前列腺癌，出现前列腺上皮内瘤、高分化腺癌、低分化癌和神经内分泌癌的病理学特点，可作为人类前列腺癌研究的经典动物模型。
基金Acknowledgement This work was supported by the ＇National Natural Science Foundation of China （Grant number 30571854） and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry.
文摘The most appropriate time to introduce androgen deprivation therapy for prostate cancer remains controversial. Our aim was to evaluate the effects of early versus delayed surgical castration on prostate cancer progression and survival in the transgenic adenocarcinoma of the mouse prostate （TRAMP） model. TRAMP mice were randomly divided into three groups： the early castration group （on which castration was performed at the age of 4 weeks）, the delayed castration group （on which castration was performed when abdominal tumours could be palpated）, and the sham-castrated group. Mice were monitored daily throughout their lives until cancer-related death or the develop- ment of an obviously moribund appearance, at which time the individual mouse was killed. Androgen receptor expression in prostate tumours was also evaluated. The results shows that the average lifespan in early castration, delayed castration and sham-castrated groups were 54.1 weeks, 59.9 weeks and 39.1 weeks, respectively. Both early castration and delayed castration conferred a statistically significant survival advantage when compared with the sham-castrated group （P 〈 0.001）. However, the difference in lifespan between the early castration group and the delayed castration group was not statistically significant （P = 0.85）. The increase in lifespan in the TRAMP mice that received either early or delayed castration correlated with lower G/B value （genitourinary tract weight/body weight） at death than the sham-castrated mice. In conclusion, early and delayed castrations in TRAMP mice pro- longed survival to a similar extent. This finding may provide a guide for clinical practice in prostate cancer therapy.
文摘Many invasive invertebrates belong to unresolved species complexes, and have a history of misidentification and in-appropriate management. Here we detail confusion surrounding the taxonomy and identification of two dolichoderine ant species, Iridomyrmex anceps Roger and OcheteUus glaber Mayr, which are commonly cited in the literature as having been spread widely by human commerce. We provide morphometric data and images strongly suggesting that these two ＂species＂ are instead com- plexes of species that are likely native to many regions where they are purportedly introduced. We take particular interest in the status of species referred to as L anceps and O. glaber in China, as this is the most northerly distribution of both genera. We con-clude by suggesting that many identifications of both species are likely to be unreliable, and we recommend against any manage- ment action aimed at controlling these species under the assumption that they are exotic [Current Zoology 57 （5）： 662-667, 2011].
文摘The effect of tramp elements in the steel was intensively studied. It was found that the solubility of tramp elements decreased as the temperature decreased under normal cooling conditions. The tramp elements （Cu, Pb, and Sn） diffused toward the grain boundaries, and intermetallic compounds or rich phases which have low melting points were formed, causing reduction in ductility and failure during the bending test. Rebars with Cu content which were left to air cooling after the last step showed drop in elongation, up to 32 %. On contrast, the samples with high per- centage of tramp elements （Cu, Pb, and Sn） in the billet, which were rolled and subjected to Tempcore process, did not show drop in elongation or failure in bending test （especially for rebar with diameter less than 32 mm）; however, copper must be less than 0.35 mass% to prevent the precipitation of Cu-rich zones of critical size in 32 mm. When quench- ing was applied, the tramp elements remained in the interstitial supersaturated solid solution positions inside the grains and would not have the chance to diffuse and form precipitates, hindering the copper precipitates from reac- hing the critical size necessary for impairing the properties. This would hinder the occurrence of the harmful effect of the tramp elements on the elongation or the hot shortness after rolling.
文摘In the thermodynamic evaluation Y was selected as a typical heavy rare earth metal for reducing the detrmental effects of tramp elements in the petroleum pipe steel. The possibilities of reactions between Y and trampelements such as Sn, Sb, As, etc. after deoxidation and desulphurization were estimated by calculating the Gibbsfree energies of formation. The equilibrium content of Y for eliminating the detrimental effects of tramp elements inthe steel was recommended from the results of thermodynamic calculation.
文摘Diets rich in soy products may reduce the risk of prostate cancer (PCa). Daidzein, the major isoflavone present in soy germ, can be metabolized by the gut microbiota into equol. The effects of daidzein and equol on PCa have not been well studied. The objective of this study was to investigate the effect of feeding 2% soy germ, 92 ppm daidzein, or 88 ppm equol diets on the progression of PCa in the transgenic adenocarcinoma of the mouse prostate (TRAMP) model. 3-week old male C57BL/6 X FVB TRAMP mice were weaned from our breeding colony and immediately acclimated to an AIN-93G control diet for one week. At 4 weeks of age, mice (n = 30 per diet group) were randomized to one of four pelleted study diets until 18 weeks of age. Unexpectedly, we did not detect any statistical differences in cancer incidence between diets. We suggest that these results are due to the physical attributes of the pelleted diets in the current study. Mice fed pelleted diets had reduced food intake and significantly decreased body weights (p < 0.001) compared to previous studies. A reduction in food intake is known to reduce cancer incidence in a number of cancer models and is likely to have contributed to the decrease in expected cancer incidence in the current study. In conclusion, we suggest that the hardness of the diets pellets could result in a decreased cancer incidence in TRAMP mice.
文摘Most disturbed habitats in the tropics and subtropics harbor numerous species of invasive ants, and occasionally the same species has been introduced repeatedly from multiple geographical sources. We examined how experimental crossbreeding between sexuals from different populations affects the fitness of queens of the tramp ant Cardiocondyla itsukii, which is widely distributed in Asia and the Pacific Islands. Eggs laid by queens that mated with nestmate males had a higher hatchi ng rate than eggs laid by queens mated to males from neighboring (Hawaii x Kauai) or distant introduced populations (Hawaii/Kauai x Okinawa). Furthermore, inbreeding queens had a Ion ger lifespan and produced a less female-biased offspring sex ratio than queens from allopatric mating. This suggests that the genetic divergence between different source populations may already be so large that in case of multiple invasions eventual crossbreeding might negatively affect the fitness of tramp ants.
文摘Tramp-tolerance characters of C299 of Cynodon dactylon (L.) Pers. and two hybrids ‘Tifdwarf’ and ‘Tifgreen’ of C. dactylon×C. transvaalensis Burtt-Davy were evaluated under the light-tramp, medium-tramp and serious-tramp with a 75 kg tramper. The results indicated that tramp caused leaf blades of C299 narrower, color lighter and turf quality lower; but the turf height and internode length of C299 with different rolling intensity were not obviously different from that of ‘Tifgreen’. The turf quality of C299 under serious tramp is higher than that of ‘Tifgreen’ and ‘Tifdwarf’. C299 had stronger ability to recover in vertical direction than that of ‘Tifgreen’ and ‘Tifdwarf’ under the tramp. The results show that C299 has the high turf quality and tramp tolerance charater.
