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Astrometric Reduction of Saturnian Satellites with Cassini-ISS Images Degraded by Trailed Stars
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作者 Qing-Feng Zhang Meng-Qi Liu +4 位作者 Yan Li Lin-Peng Wu Zhi-Qiang Wang Li-Sha Zhu Zhan Li 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2023年第3期85-92,共8页
The Imaging Science Subsystem(ISS)mounted on the Cassini spacecraft has taken a lot of images,which provides an important source of high-precision astrometry of some planets and satellites.However,some of these images... The Imaging Science Subsystem(ISS)mounted on the Cassini spacecraft has taken a lot of images,which provides an important source of high-precision astrometry of some planets and satellites.However,some of these images are degraded by trailed stars.Previously,these degraded images cannot be used for astrometry.In this paper,a new method is proposed to detect and compute the centers of these trailed stars automatically.The method is then performed on the astrometry of ISS images with trailed stars.Finally,we provided 658 astrometric positions between 2004 and 2017 of several satellites that include Enceladus,Dione,Tethys,Mimas and Rhea.Compared with the JPL ephemeris SAT427,the mean residuals of these measurements are 0.11 km and 0.26 km in R.A.and decl.,respectively.Their standard deviations are 1.08 km and 1.37 km,respectively.The results show that the proposed method performs astrometric measurements of Cassini ISS images with trailed stars effectively. 展开更多
关键词 STARS TRAIL SATURN
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携带TRAIL基因的P28启动子溶瘤腺病毒的抗肝癌研究
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作者 金晓婷 刘新垣 +2 位作者 陈若愚 李宁 章康健 《中国细胞生物学学报》 2025年第4期767-776,共10页
P28(也被称为PSMD10、P28^(GANK)或gankyrin)在肝癌中高表达(96.9%)而在正常细胞中不表达,因此利用P28启动子设计了具有肝癌靶向性的新型溶瘤病毒载体并在此基础上设计了携带肿瘤坏死因子(tumor necrosis factor,TNF)相关凋亡诱导配体(T... P28(也被称为PSMD10、P28^(GANK)或gankyrin)在肝癌中高表达(96.9%)而在正常细胞中不表达,因此利用P28启动子设计了具有肝癌靶向性的新型溶瘤病毒载体并在此基础上设计了携带肿瘤坏死因子(tumor necrosis factor,TNF)相关凋亡诱导配体(TNF related apoptosis inducing ligand,TRAIL)基因的溶瘤腺病毒(Onco^(Ad)-P28-E1A-?E1B-TRAIL)。通过Onco^(Ad)-P28-TRAIL感染肝癌细胞显示TRAIL蛋白可以在肝癌细胞中高效表达。结晶紫染色显示该病毒可以选择性靶向杀伤肝癌细胞,且10 MOI对人正常肝细胞没有明显毒性。体外细胞水平杀伤及凋亡研究显示Onco~(Ad)-P28-TRAIL对肝癌细胞Huh-7和Hep-3B均有抑制作用,且呈时间和剂量依赖性,可以显著促进肝癌细胞凋亡。皮下移植瘤CDX药效显示该溶瘤腺病毒可以促进肿瘤细胞的凋亡及免疫细胞的浸润从而显著抑制肝癌细胞皮下瘤的生长。这些结果提示溶瘤腺病毒Onco^(Ad)-P28-TRAIL能有效抑制肝癌的发展,是一种具有临床应用潜力的肝癌治疗候选药物。 展开更多
关键词 P28启动子 TRAIL 溶瘤腺病毒 肝癌
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编委推荐
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《遗传》 北大核心 2025年第12期1285-1286,共2页
Cell|低糖饮食通过外泌体TRAIL介导形成免疫抑制微环境促进肿瘤肺转移 葡萄糖代谢重编程是肿瘤恶性特征之一。有临床前证据提示,低碳水化合物饮食或糖酵解抑制剂在缩小原发灶的同时,可能意外播下肿瘤远处转移的“种子”。2025年7月15日... Cell|低糖饮食通过外泌体TRAIL介导形成免疫抑制微环境促进肿瘤肺转移 葡萄糖代谢重编程是肿瘤恶性特征之一。有临床前证据提示,低碳水化合物饮食或糖酵解抑制剂在缩小原发灶的同时,可能意外播下肿瘤远处转移的“种子”。2025年7月15日,中山大学邝栋明、魏瑗团队在Cell在线发表了题为“Glucose restriction shapes pre-metastatic innate immune landscapes in the lung through exosomal TRAIL”的研究论文(doi:10.1016/j.cell.2025.06.027),破除了这一矛盾。 展开更多
关键词 外泌体TRAIL 低糖饮食 免疫抑制微环境 Cell
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探秘中亚最长徒步路线
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作者 王玲燕 《疯狂英语(新悦读)》 2025年第9期38-40,78,共4页
主题语境:旅游探险,篇幅:330词,建议用时:6分钟,For thrill-seekers and nature lovers,Kyrgyzstan just got a whole lot more exciting!The Kyrgyz Nomad Trail(KNT)is an incredible 2,000⁃kilometer hiking route that stretches ac... 主题语境:旅游探险,篇幅:330词,建议用时:6分钟,For thrill-seekers and nature lovers,Kyrgyzstan just got a whole lot more exciting!The Kyrgyz Nomad Trail(KNT)is an incredible 2,000⁃kilometer hiking route that stretches across northern and eastern Kyrgyzstan,making it Central Asias longest trail network.Launched in June 2024,this adventure trail weaves through breathtaking landscapes,passing through regions like Jalal-Abad,Naryn and Issyk-Kul. 展开更多
关键词 kyrgyz nomad trail knt nature lovers HIKING kilometer KYRGYZSTAN kyrgyz nomad trail thrill seekers central asia
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Interference mechanism of trailing edge flap shedding vortices with rotor wake and aerodynamic characteristics
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作者 Yang LIU Yongjie SHI +2 位作者 Aqib AZIZ Guohua XU Haifeng GAO 《Chinese Journal of Aeronautics》 2025年第1期325-339,共15页
A robust Reynolds-Averaged Navier-Stokes(RANS)based solver is established to predict the complex unsteady aerodynamic characteristics of the Active Flap Control(AFC)rotor.The complex motion with multiple degrees of fr... A robust Reynolds-Averaged Navier-Stokes(RANS)based solver is established to predict the complex unsteady aerodynamic characteristics of the Active Flap Control(AFC)rotor.The complex motion with multiple degrees of freedom of the Trailing Edge Flap(TEF)is analyzed by employing an inverse nested overset grid method.Simulation of non-rotational and rotational modes of blade motion are carried out to investigate the formation and development of TEF shedding vortex with high-frequency deflection of TEF.Moreover,the mechanism of TEF deflection interference with blade tip vortex and overall rotor aerodynamics is also explored.In nonrotational mode,two bundles of vortices form at the gap ends of TEF and the main blade and merge into a single TEF vortex.Dynamic deflection of the TEF significantly interferes with the blade tip vortex.The position of the blade tip vortex consistently changes,and its frequency is directly related to the frequency of TEF deflection.In rotational mode,the tip vortex forms a helical structure.The end vortices at the gap sides co-swirl and subsequently merge into the concentrated beam of tip vortices,causing fluctuations in the vorticity and axial position of the tip vortex under the rotor.This research concludes with the investigation on suppression of Blade Vortex Interaction(BVI),showing an increase in miss distance and reduction in the vorticity of tip vortex through TEF phase control at a particular control frequency.Through this mechanism,a designed TEF deflection law increases the miss distance by 34.7%and reduces vorticity by 11.9%at the target position,demonstrating the effectiveness of AFC in mitigating BVI. 展开更多
关键词 CFD ROTOR Aerodynamic Trailing edge flap Vortex shedding BVI
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文化编织者——品牌如何把赛道变成信仰
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作者 《中国自行车》编辑部 《中国自行车》 2025年第6期52-61,共10页
当一条条赛道落地生根,谁在背后编织它们的灵魂?或许是品牌。品牌不再仅是设备的提供者,还是文化基础设施的共建者,以长期主义的心态,投入资金、技术与愿景,将骑行转化为一种信仰。禧玛诺通过“Trail Born基金”在中国播种林道文化;闪... 当一条条赛道落地生根,谁在背后编织它们的灵魂?或许是品牌。品牌不再仅是设备的提供者,还是文化基础设施的共建者,以长期主义的心态,投入资金、技术与愿景,将骑行转化为一种信仰。禧玛诺通过“Trail Born基金”在中国播种林道文化;闪电借“寻山辟路”计划深化骑行社群的联结,拓宽运动的边界。品牌的文化基建之战,早已超越商业竞争,进入价值输出的新阶段。 展开更多
关键词 信仰 品牌 赛道 Trail Born基金 长期主义 禧玛诺
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Thematic Tour Interactive Experience Platform Empowered by Dynamic Path Planning and Augmented Reality:An Empirical Study on Enhancing Visitor Engagement and Cultural Identity
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作者 Li Shen Yueying Huang +1 位作者 Jinxin Wang Xiaowen Ye 《Journal of Electronic Research and Application》 2025年第6期427-434,共8页
This study focuses on the application of dynamic route planning and augmented reality(AR)technology within interactive theme trail platforms.Taking the“Dongpo Travelogue”digital guide mini-program as a case study,it... This study focuses on the application of dynamic route planning and augmented reality(AR)technology within interactive theme trail platforms.Taking the“Dongpo Travelogue”digital guide mini-program as a case study,it employs empirical analysis to explore its impact on enhancing visitor engagement and cultural identification.Employing a combined quantitative and qualitative methodology,the study analyses the platform’s functional implementation in route planning,cultural narration,interactive games,and their impact on visitor experience.Findings indicate that the integration of dynamic route planning and AR technology significantly enhances visitor engagement and cultural identity,offering novel insights for the digital transformation of the cultural tourism industry. 展开更多
关键词 Dynamic route planning Themed trails Interactive experience Cultural identity
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Verification of the Lattice Spring Model for Studying on the Incline Angle of a Three-Segment Towed Array
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作者 GUO Ru-qian MIAO Qiu-yan +3 位作者 YAN Guo-feng JIANG Lang WU Qi XIAO Chun 《China Ocean Engineering》 2025年第4期718-727,共10页
This study introduces the lattice spring model(LSM)to investigate the incline angle of a non-uniform three-segment towed array under steady-state conditions.A numerical model was established,and parametric analysis wa... This study introduces the lattice spring model(LSM)to investigate the incline angle of a non-uniform three-segment towed array under steady-state conditions.A numerical model was established,and parametric analysis was conducted to examine the effects of towing speed and cable density on the incline angle.The numerical simulations demonstrate that for a conventional three-segment towed array with heavy vibration-isolation cable and density exceeding that of seawater,the towing speed must exceed 4 kn to maintain the acoustic cable's average incline angle below 10°.To validate the proposed LSM,a 100-meter-long towed array with variable densities was fabricated and tested through lake trials.The experimental results align closely with simulations,confirming LSM as a reliable model for predicting towed array position and posture.The study concludes by analyzing the parallel computing capabilities of LSM and its application in Fluid-Structure Interaction(FSI)problems.The model's precision and parallel computing capabilities make LSM an efficient,reliable tool for analyzing the steady-state behavior of towed systems. 展开更多
关键词 incline angle towed array lattice spring model lake trail
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Design and aerodynamic characteristic analysis of flexible morphing wing based on cellular structure
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作者 Kexin ZHENG Daochun LI +4 位作者 Zi KAN Kai MA Ziyu WANG Lu ZHANG Jinwu XIANG 《Chinese Journal of Aeronautics》 2025年第9期166-176,共11页
This paper aims to design a morphing wing with both Flexible Leading Edge(FLE)and Flexible Trailing Edge(FTE)by using cellular structures,which can help the wing boost the deformation to a greater extent on the premis... This paper aims to design a morphing wing with both Flexible Leading Edge(FLE)and Flexible Trailing Edge(FTE)by using cellular structures,which can help the wing boost the deformation to a greater extent on the premise that the weight is not changed,so as to play a greater role in aerodynamic control such as gust interference.First,as for structural design,based on NACA0012,a morphing wing model constructed by 3 forms of cell structures is proposed.Then,the aerodynamic characteristics under the interference of FLE and FTE are calculated by the Computational Fluid Dynamic(CFD)method.After the surrogate model is established to predict the lift coefficient of the wing effectively,the sensitivity analysis reveals that the main sensitivity index of FTE deflection angle β is 0.565,which has the greatest influence on the lift coefficient.And the total sensitivity index of FLE deflection angle γ is increased by 78.9%,which reveals a strong coupling relationship between FLE and FTE.Finally,using Finite Element Analysis(FEA)method and experiment,the deformation capability of the model under certain static loads are obtained.The results reveal that the maximum deflection angle of the morphing wing model can be±22°at FLE and±64°at FTE,indicating strong structural stiffness and resistance to bending breakage of the model.The presented results can be useful in the design of the cellular morphing wing with multiple flexible systems. 展开更多
关键词 Cellular structure Flexible leading edge Flexible trailing edge Morphing wing Surrogate model
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Vortex-Induced Vibration and Frequency Lock-In of an Elastically Suspended Hydrofoil with Blunt Trailing Edge
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作者 QIN Guangfei ZHANG Huaixin LI Date 《Journal of Shanghai Jiaotong university(Science)》 2025年第6期1289-1298,共10页
Vortex-induced vibration of hydrofoils is concerned with structural safety and noise level in hydraulic machinery and marine engineering.The research on vibration characteristics under different operating conditions i... Vortex-induced vibration of hydrofoils is concerned with structural safety and noise level in hydraulic machinery and marine engineering.The research on vibration characteristics under different operating conditions is significant.In this study,numerical simulations are conducted to investigate the vortex-induced vibration responses of an elastically suspended hydrofoil with blunt trailing edge in pitch direction.The work studies the effects of four parameters,namely the structural natural frequency,mass ratio,initial attack angle,and Reynolds number on vibration characteristics,with special emphasis on frequency lock-in.Results indicate that as the structural natural frequency changes,the vibration amplitude may increase substantially within a certain frequency range,in which the vortex shedding frequency locks into the structural natural frequency,and frequency lock-in occurs.In addition,with increasing the mass ratio,the frequency range of lock-in becomes narrower,and both the upper and lower thresholds decrease.As the initial attack angle increases from 0◦to 6◦,the lock-in range gets reduced.Over the three Reynolds numbers(6×10^(5),9×10^(5),and 12×10^(5)),the lock-in range remains virtually unchanged.Moreover,for a certain structural natural frequency,modifying the mass ratio,initial attack angle,and Reynolds number could effectively suppress the vibration amplitude. 展开更多
关键词 vortex-induced vibration HYDROFOIL blunt trailing edge frequency lock-in structural natural frequency mass ratio initial attack angle Reynolds number
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In Pursuit of Performance
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作者 GE LIJUN 《ChinAfrica》 2025年第9期25-27,共3页
On a summer morning in Boqiang Village,tucked beneath the emerald mountains of Fanshi County,Shanxi Province,the Ultra Trail Mount Wutai 2025 commenced with the sound of the starting pistol-an event that was much more... On a summer morning in Boqiang Village,tucked beneath the emerald mountains of Fanshi County,Shanxi Province,the Ultra Trail Mount Wutai 2025 commenced with the sound of the starting pistol-an event that was much more than just a competition.As soon as the colourful smoke bombs lit up the sky,some 2,000 runners from China and abroad set off to reach one of the country’s most sacred Buddhist peaks.Over the past decade,this race has become a fixture on the global running calendar and a symbol of the growing popularity of endurance sports. 展开更多
关键词 smoke bombs Running Competition Ultra Trail Mount Wutai Global Running Calendar Endurance Sports Sacred Buddhist Peaks
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Bioinspired Trailing Edge Serrations for Vertical Axis Wind Turbine Blades in Urban Environments: Performance Effects
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作者 Luis Santamaria Laura Suarez Fernandez +2 位作者 Manuel Garcia-Diaz Jose Gonzalez Perez Monica Galdo 《Journal of Bionic Engineering》 2025年第2期822-837,共16页
Biomimetics has recently emerged as an interesting approach to enhance renewable energy technologies.In this work,bioinspired Trailing Edge Serrations(TES)were evaluated on a typical Vertical Axis Wind Turbine(VAWT)ai... Biomimetics has recently emerged as an interesting approach to enhance renewable energy technologies.In this work,bioinspired Trailing Edge Serrations(TES)were evaluated on a typical Vertical Axis Wind Turbine(VAWT)airfoil,the DU06-W200.As noise reduction benefits of these mechanisms are already well-established,this study focuses on their impact on airfoil and VAWT performance.A saw-tooth geometry was chosen based on VAWT specifications and existing research,followed by a detailed assessment through wind tunnel tests using a newly developed aerodynamic balance.For a broad spectrum of attack angles and Reynolds numbers,lift,drag,and pitching moments were carefully measured.The results show that TES enhance the lift-to-drag ratio,especially in stalled conditions,and postpone stall at negative angles,expanding the effective performance range.A notable increase in pitching moment also is also observed,relevant for blade-strut joint design.Additionally,the impact on turbine performance was estimated using an analytical model,demonstrating excellent accuracy when compared against previous experimental results.TES offer a modest 2%improve-ment in peak performance,though they slightly narrow the optimal tip-speed ratio zone.Despite this,the potential noise reduction and performance gains make TES a valuable addition to VAWT designs,especially in urban settings. 展开更多
关键词 Biomimetics Trailing edge serrations Passive flow control device Vertical axis wind turbine VAWT Wind tunnel DU06-W200
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Call for Papers─Feature Topic Vol.23,No.2,2026 Beyond Terrestrial Infrastructure: Meteor Burst Communications for Next-Generation Emergency Communication Networks
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《China Communications》 2025年第8期I0002-I0002,共1页
Meteor Burst Communication(MBC),a niche yet revolutionary wireless communication paradigm,exploits the transient ionized trails generated by meteors ablating in Earth’s atmosphere to enable sporadic yet resilient lon... Meteor Burst Communication(MBC),a niche yet revolutionary wireless communication paradigm,exploits the transient ionized trails generated by meteors ablating in Earth’s atmosphere to enable sporadic yet resilient long-distance radio links.Known for its exceptional resilience,robustness,and sustained connectivity,MBC holds significant promise for applications in emergency communications,remote area connectivity,military/defense systems,and environmental monitoring.However,the scientific exploration and application of MBC have long been highly challenging.In particular,under the combined influence of multiple physical field factors,the channel experiences superimposed multiple random fading effects,exhibiting bursty,highly time-varying,and strongly random characteristics.This persistent technical challenge has resulted in the absence of a practical statistical channel model for MBC to date. 展开更多
关键词 wireless communication paradigmexploits earth s atmosphere Resilient Long Distance Radio Links Emergency Communication Networks Meteor Burst Communications emergency communicationsremote area connectivitymilitary defense transient ionized trails meteor burst communication mbc
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Fas FasL TRAIL分子在尖锐湿疣皮损中的分布 被引量:19
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作者 谢红付 冯浩 +3 位作者 范学工 肖满兰 陈翔 施为 《中国皮肤性病学杂志》 CAS 北大核心 2003年第3期151-153,共3页
目的 探讨Fas、FasL和TRAIL介导的细胞凋亡在尖锐湿疣发病中的可能作用及意义。方法 用免疫组化方法检测了 30例尖锐湿疣患者皮损中Fas、FasL和TRAIL的表达。结果 所有尖锐湿疣标本 ( 10 0 % )Fas均阳性 ,2 3例 ( 76 .7% )FasL阳性 ,... 目的 探讨Fas、FasL和TRAIL介导的细胞凋亡在尖锐湿疣发病中的可能作用及意义。方法 用免疫组化方法检测了 30例尖锐湿疣患者皮损中Fas、FasL和TRAIL的表达。结果 所有尖锐湿疣标本 ( 10 0 % )Fas均阳性 ,2 3例 ( 76 .7% )FasL阳性 ,2 1例 ( 70 .0 % )TRAIL阳性 ,三者分布基本一致 ,正常表皮基本不表达。且FasL和TRAIL分子与真皮单一核细胞浸润呈显著负相关 (γ =-0 .5 0 6和γ =-0 .45 0 ,P均 <0 .0 5 )。结论 尖锐湿疣组织中细胞凋亡相关分子Fas、FasL和TRAIL表达异常 ,且Fas、TRAIL分子表达影响局部免疫反应 ,考虑凋亡失控可能参与尖锐湿疣发病 ,引起复发。 展开更多
关键词 FAS FASL TRAIL 尖锐湿疣 皮损 细胞凋亡 人乳头瘤病毒
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TRAIL受体在肿瘤细胞系上的表达及意义 被引量:8
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作者 韩丽辉 孙汶生 +3 位作者 马春红 张利宁 曹英林 宋静 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2002年第2期128-130,共3页
目的  检测TNF相关凋亡诱导配体(TRAIL)的受体,在来源于血液系统、肝脏、肺脏和大肠的8个肿瘤细胞系中的表达,并探讨其意义。方法采用半定量RT-PCR,对TRAIL受体的表达进行半定量检测。结果 TRAIL凋亡... 目的  检测TNF相关凋亡诱导配体(TRAIL)的受体,在来源于血液系统、肝脏、肺脏和大肠的8个肿瘤细胞系中的表达,并探讨其意义。方法采用半定量RT-PCR,对TRAIL受体的表达进行半定量检测。结果 TRAIL凋亡通路中,能够诱导凋亡反应的死亡受体DR4和DR5,在所检测的肿瘤细胞系中都有表达,其中DR5在所有肿瘤细胞系中的表达水平均显著高于DR4(P<0.05)。而能够竞争性阻断TRAIL诱导的凋亡反应的诱骗受体DcR1和DcR2,在所有的肿瘤细胞中都呈低水平表达或不表达。结论 DR5可能在TRAIL诱导凋亡的通路中发挥最重要的作用。TRAIL死亡受体和诱骗受体在肿瘤细胞系中的表达具有差异性,这种差异性可在一定程度上解释不同细胞对TRAIL诱导凋亡的敏感度。 展开更多
关键词 肿瘤 RT-PCR TRAIL TRAIL受体
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抑制Livin基因对肺腺癌SPC-A1细胞增殖及顺铂敏感性的影响 被引量:16
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作者 庄莉 董超 +1 位作者 杨润祥 高春林 《现代生物医学进展》 CAS 2015年第6期1036-1040,共5页
目的:Livin是近年来发现的人类凋亡抑制蛋白(inhibitorof apoptosis protein,IAP)家族的新成员,发现在多数肿瘤中表达,与肿瘤发生有密切关系,并可能作为肿瘤诱导凋亡治疗的新靶点。本研究旨在探讨si RNA-Livin和夫拉平度(Flavopiridol,... 目的:Livin是近年来发现的人类凋亡抑制蛋白(inhibitorof apoptosis protein,IAP)家族的新成员,发现在多数肿瘤中表达,与肿瘤发生有密切关系,并可能作为肿瘤诱导凋亡治疗的新靶点。本研究旨在探讨si RNA-Livin和夫拉平度(Flavopiridol,FP)协同凋亡诱导配体(TRAIL)两种方式有效抑制Livin表达,诱导非小细胞肺癌SPC-A1凋亡并增强对化疗药物顺铂的敏感性。方法:si RNA-Livin转染SPC-A1,Real-Time PCR检测Livin基因的表达水平,MTT检测干扰组和干扰加药组肿瘤细胞的增殖及活性;TRAIL、FP单独及联合作用诱导细胞凋亡,蛋白质印迹法检测凋亡抑制蛋白Livin的表达水平,MTT检测各处理组细胞的增殖及活性。结果:100 nmol/LFP处理组(F)细胞存活率为(84.30±1.34)%,100 ng/m LTRAIL处理组(T)为(93.40±1.56)%,FP和TRAIL联合组(F+T)为(48.02±1.35)%,si RNA-Livin处理组为(50.88±1.14)%,1.2μg/m L Cisplatin处理组为(19.30±0.89)%,si RNA-livin+Cisplatin组为(14.37±0.81)%,FP+T+Cisplatin组为(10.86±0.87)%,C组存活率为100%。F+T组对细胞的增殖抑制作用显著高于单独用药组,si RNA-livin+Cisplatin与si RNA-Livin组相比、FP+T+Cisplatin与FP+T组相比都显著增强了化疗药物对SPC-A1的杀伤作用。50μmol/LZ-VAD-FMK预处理后联合用药组细胞的存活率为(88.16±1.64)%,caspase抑制剂能明显抑制F+T联合处理组的凋亡效应。结论:RNA干扰和F+T联合用药都能显著降低凋亡抑制蛋白Livin的表达,有效抑制肿瘤细胞的增殖生长,并增强肿瘤细胞对化疗药物顺铂的敏感性,为肺腺癌的靶向治疗提供新的理论依据。 展开更多
关键词 LIVIN RNA干扰 FLAVOPIRIDOL TRAIL 顺铂 SPC-A1
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Meta分析系列之三:随机对照试验的质量评价工具 被引量:265
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作者 曾宪涛 包翠萍 +1 位作者 曹世义 刘菊英 《中国循证心血管医学杂志》 2012年第3期183-185,共3页
Meta分析是对原始研究的二次综合分析与评价,其质量受纳入原始研究质量、评价的方法、评价者的知识水平及观点的影响.若Meta分析纳入的原始研究质量低,且未对原始研究方法学质量进行正确评价,Meta分析的结果和结论可能是不正确的,从而... Meta分析是对原始研究的二次综合分析与评价,其质量受纳入原始研究质量、评价的方法、评价者的知识水平及观点的影响.若Meta分析纳入的原始研究质量低,且未对原始研究方法学质量进行正确评价,Meta分析的结果和结论可能是不正确的,从而对临床实践造成误导[1].因此,对Meta分析纳入的原始研究质量进行严格的评价尤为重要.原始研究质量评价工具主要根据研究设计、实施、结果分析整个过程中可能出现偏倚的各个因素而确定,目前出现的评价工具较多,主要有单个评价条目(components/items)、清单(checklist/list)和量表(scale)三种类型.前面我们已经对Meta分析的类型[2]、Meta分析的软件[3]进行了介绍,本文将在简要回顾原始研究类型的基础上,对随机对照试验(randomized controlled trail,RCT)常用的质量评价工具进行简要分析. 展开更多
关键词 META分析 随机对照试验 评价工具 质量 研究方法学 TRAIL 知识水平 临床实践
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重组可溶性TRAIL的表达与生物学活性 被引量:16
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作者 史娟 张锦春 +3 位作者 畅晓燕 刘士廉 刘彦信 郑德先 《中国生物工程杂志》 CAS CSCD 2003年第6期46-49,共4页
研究重组可溶性TRAIL的抗肿瘤生物学活性。采用基因分子生物学方法构建重组可溶性TRAIL的表达载体 ,建立大肠杆菌表达菌株 ,采用柱层析等方法获得纯化的重组可溶性TRAIL ;采用流式细胞术、细胞活性测定法和体内药效学实验分析重组可溶性... 研究重组可溶性TRAIL的抗肿瘤生物学活性。采用基因分子生物学方法构建重组可溶性TRAIL的表达载体 ,建立大肠杆菌表达菌株 ,采用柱层析等方法获得纯化的重组可溶性TRAIL ;采用流式细胞术、细胞活性测定法和体内药效学实验分析重组可溶性TRAIL杀伤肿瘤细胞的生物学活性。结果表明重组可溶性TRAIL在体外可诱导人白血病细胞和肝癌细胞凋亡 ,凋亡率达5 0 %以上。中枢神经系统的肿瘤细胞对重组可溶性TRAIL不敏感。重组可溶性TRAIL在体内能显著抑制人非小细胞肺癌细胞在小鼠体内生长 ,抑制率达 70 %以上。结论为研究制备的重组可溶性TRAIL能在体内外杀死多种肿瘤细胞 ,具有显著的临床应用前景。 展开更多
关键词 重组可溶性TRAIL 表达 生物学活性 肿瘤细胞凋亡
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腺病毒载体携带人TRAIL基因治疗H446小细胞肺癌的体外实验研究 被引量:9
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作者 刘永靖 陈飞虎 +3 位作者 于奇 韩继彪 苏长青 钱其军 《中国药理学通报》 CAS CSCD 北大核心 2007年第6期755-759,共5页
目的评价携带人TRAIL基因的腺病毒载体对人小细胞肺癌细胞株H446的基因治疗功效。方法构建的携带人TRAIL基因的腺病毒Ad-hTRAIL,感染人小细胞肺癌细胞株H446以及人正常肝细胞株WRL-68,人正常成纤维细胞株MRC-5,通过四甲基偶氮唑蓝(MTT)... 目的评价携带人TRAIL基因的腺病毒载体对人小细胞肺癌细胞株H446的基因治疗功效。方法构建的携带人TRAIL基因的腺病毒Ad-hTRAIL,感染人小细胞肺癌细胞株H446以及人正常肝细胞株WRL-68,人正常成纤维细胞株MRC-5,通过四甲基偶氮唑蓝(MTT)比色法检测其杀伤肿瘤细胞的效能,流式细胞术(FCM)检测Ad-hTRAIL对细胞早期凋亡的影响,并进行RT-PCR、酶联免疫吸附试验(ELISA)检测TRAIL mRNA和TRAIL蛋白的表达量。结果携带人TRAIL基因的增殖缺陷型腺病毒Ad-hTRAIL对H446细胞的感染效率较高;感染72h后H446、WRL-68、MRC-5细胞培养上清中TRAIL的表达量分别为32.02、17.08、16.89μg.L-1,在MOI=1.0时,Ad-hTRAIL即可引起H446细胞明显凋亡;而在MOI=100时对正常细胞WRL-68、MRC-5也没有明显杀伤作用。结论Ad-hTRAIL能够强烈诱导小细胞肺癌细胞H446凋亡而对正常肝细胞及成纤维细胞无明显抑制作用,Ad-hTRAIL在小细胞肺癌的基因治疗方面有潜在的应用前景。 展开更多
关键词 小细胞肺癌 基因治疗 腺病毒 TRAIL 凋亡
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TRAIL蛋白的表达、纯化和抗肿瘤活性 被引量:14
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作者 李小安 房殿春 +1 位作者 杨仕明 罗元辉 《第三军医大学学报》 CAS CSCD 北大核心 2001年第9期1058-1060,共3页
目的 利用大肠杆菌表达出TRAIL蛋白 ,并观察分离纯化后的TRAIL蛋白的抗肿瘤活性。方法 采用CaCl2 法将带有TRAIL(氨基酸 114 -2 81,含六聚组氨酸尾 )基因的pET d质粒转入大肠杆菌BL2 1(DE3)pLysS ,IPTG诱导蛋白表达 ;使用Ni NTA层析... 目的 利用大肠杆菌表达出TRAIL蛋白 ,并观察分离纯化后的TRAIL蛋白的抗肿瘤活性。方法 采用CaCl2 法将带有TRAIL(氨基酸 114 -2 81,含六聚组氨酸尾 )基因的pET d质粒转入大肠杆菌BL2 1(DE3)pLysS ,IPTG诱导蛋白表达 ;使用Ni NTA层析柱分离纯化蛋白 ,SDS PAGE和Westernblot法鉴定TRAIL蛋白的表达 ;MTT法检测TRAIL蛋白的抗肿瘤活性。结果 我们分离纯化出分子量为 2 0 .1× 10 3 的蛋白 ,Westernblot法证实为TRAIL蛋白 ,MTT法检测出其有较高的抗肿瘤活性 ,呈现出较好的时效和量效关系。结论 成功地利用大肠杆菌表达、Ni NTA层析柱分离纯化出具有抗肿瘤活性的TRAIL蛋白 。 展开更多
关键词 TRAIL蛋白 大肠杆菌 抗肿瘤活性 药理作用 抗癌药
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