OBJECTIVE Nowadays,with the living habits and diet structure changes,the morbidity and mortality of hypertension has been rising yearly,which induced by adverse lifestyles,excessive alcohol and poor diet.However,the a...OBJECTIVE Nowadays,with the living habits and diet structure changes,the morbidity and mortality of hypertension has been rising yearly,which induced by adverse lifestyles,excessive alcohol and poor diet.However,the animal models of hypertension resulted from adverse lifestyles have rarely been reported.It is essential to set up some more systematic models to study the characters of hypertension and evaluate the efficacy of traditional Chinese medicine(TCM).METHODS To build the rat model of human lifehabitual hypertension,SD rats had free access to alcohol with high-sucrose-fat diet(AHSFD),alcohol with high-fat diet(AHFD),high-sucrose-fat diet(HSFD),high-fat diet(HFD)and alcohol etc.Various of indicators were detected systematically to explore the features of hypertension models which included blood pressure(BP),liver and kidney functions,the lipid profiles and indexes quantifying TCM symptoms.Then we choose one of the stable and sustained animal models,studying the effects of RPA.E(which extracted from a TCM)on modulating the level of hypertension and the preliminary mechanism in this abstract.RESULTS ①The BP level,serum TC,TG,LDL-C of AHSFD-induced and AHFD-induced rats increased significantly,while the HDL-C reduced in the 4th week.② The BP level HSFD-induced rats increased significantly in the 6th week,serum TC,TG increased in 10 th week.③ The BP level of alcohol-induced rats increased significantly in the 9th week,serum TC,TG increased in 24 th week.④ The level of serum ALT,AST,UA and Cr of all model rats increased significantly after 12 weeks.Meanwhile the microcirculation increased significantly after8 weeks.⑤ After 4-week administration,RPA.E could significantly reduce BP of AHFD-induced hypertensive rats,and could reduce the serum TC,LDL-C,HDL-C,ALT,AST level after 8-week.CONCLUSION The rats induced by AHSFD,AHFD,HSFD and alcohol can raise BP obviously with underlying hepatic and kidney damage,the lipid profiles disorder and TCM symptoms change,while the AH(S)FD-induced hypertension models are earlier and more stable.RPA.E could mildly reduce BP level,and improve the lipid profiles disorders,hepatic damage,which reflects the characteristics of TCM on antihypertensive effects.The above models have their own characteristics,can be used to study the causes and pathogenesis of hypertension complicating metabolic disorder and the related treatment drug screening.展开更多
Comprehensive identification of conditionally essential genes requires efficient tools for generating high-density transposon libraries that, ideally, can be analysed using next-generation sequencing methods such as T...Comprehensive identification of conditionally essential genes requires efficient tools for generating high-density transposon libraries that, ideally, can be analysed using next-generation sequencing methods such as Transposon Directed Insertion-site Sequencing (TraDIS). The Himar1 (mariner) transposon is ideal for generating near-saturating mutant libraries, especially in AT-rich chromosomes, as the requirement for integration is a TA dinucleotide, and this transposon has been used for mutagenesis of a wide variety of bacteria. However, plasmids for mariner delivery do not necessarily work well in all bacteria. In particular, there are limited tools for functional genomic analysis of Pasteurellaceae species of major veterinary importance, such as swine and cattle pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida, respectively. Here, we developed plasmids, pTsodCPC9 and pTlacPC9 (differing only in the promoter driving expression of the transposase gene), that allow delivery of mariner into both these pathogens, but which should also be applicable to a wider range of bacteria. Using the pTlacPC9 vector, we have generated, for the first time, saturating mariner mutant libraries in both A. pleuropneumoniae and P. multocida that showed a near random distribution of insertions around the respective chromosomes as detected by TraDIS. A preliminary screen of 5000 mutants each identified 8 and 14 genes, respectively, that are required for growth under anaerobic conditions. Future high-throughput screening of the generated libraries will facilitate identification of mutants required for growth under different conditions, including in vivo, highlighting key virulence factors and pathways that can be exploited for development of novel therapeutics and vaccines.展开更多
目的探讨活血化瘀中药熏药对乳腺癌改良根治术后切口愈合的影响。方法选取2022年12月至2024年12月赣南医科大学第二附属医院收治的60例行乳腺癌改良根治术治疗的乳腺癌患者作为研究对象,按照随机数字表法分为对照组与观察组,每组30例。...目的探讨活血化瘀中药熏药对乳腺癌改良根治术后切口愈合的影响。方法选取2022年12月至2024年12月赣南医科大学第二附属医院收治的60例行乳腺癌改良根治术治疗的乳腺癌患者作为研究对象,按照随机数字表法分为对照组与观察组,每组30例。对照组术后给予常规消毒及正常换药,观察组在对照组的基础上联合活血化瘀中药熏药,比较两组患者切口愈合能力(切口愈合初期、中期及完全愈合所需时间),术后疼痛程度[术后第1、4天视觉模拟评分法(VAS)评分],术前及术后炎症水平[术前1 d及术后4 d降钙素原(PCT)、C反应蛋白(CRP)]及术后治疗效果。结果两组患者术前炎症水平、术后切口愈合初期所需时间及术后第1天VAS评分比较,差异无统计学意义(P>0.05)。观察组术后切口愈合中期及完全愈合所需时间均短于对照组,术后第4天VAS评分低于对照组,术后4 d PCT、CRP水平均低于对照组,治疗总有效率高于对照组,差异有统计学意义(P<0.05)。结论活血化瘀中药熏药可有效促进乳腺癌患者改良根治术后的切口愈合,缓解患者疼痛程度,改善炎症反应,疗效显著。展开更多
基金The project supported by National Natural Science Foundation of China(81274123,81374003)National Major Scientific and Technological Specialized Project for the Significant Formulation of New Drugs,China(2011ZX09101-002-07,2009ZX09502-016)
文摘OBJECTIVE Nowadays,with the living habits and diet structure changes,the morbidity and mortality of hypertension has been rising yearly,which induced by adverse lifestyles,excessive alcohol and poor diet.However,the animal models of hypertension resulted from adverse lifestyles have rarely been reported.It is essential to set up some more systematic models to study the characters of hypertension and evaluate the efficacy of traditional Chinese medicine(TCM).METHODS To build the rat model of human lifehabitual hypertension,SD rats had free access to alcohol with high-sucrose-fat diet(AHSFD),alcohol with high-fat diet(AHFD),high-sucrose-fat diet(HSFD),high-fat diet(HFD)and alcohol etc.Various of indicators were detected systematically to explore the features of hypertension models which included blood pressure(BP),liver and kidney functions,the lipid profiles and indexes quantifying TCM symptoms.Then we choose one of the stable and sustained animal models,studying the effects of RPA.E(which extracted from a TCM)on modulating the level of hypertension and the preliminary mechanism in this abstract.RESULTS ①The BP level,serum TC,TG,LDL-C of AHSFD-induced and AHFD-induced rats increased significantly,while the HDL-C reduced in the 4th week.② The BP level HSFD-induced rats increased significantly in the 6th week,serum TC,TG increased in 10 th week.③ The BP level of alcohol-induced rats increased significantly in the 9th week,serum TC,TG increased in 24 th week.④ The level of serum ALT,AST,UA and Cr of all model rats increased significantly after 12 weeks.Meanwhile the microcirculation increased significantly after8 weeks.⑤ After 4-week administration,RPA.E could significantly reduce BP of AHFD-induced hypertensive rats,and could reduce the serum TC,LDL-C,HDL-C,ALT,AST level after 8-week.CONCLUSION The rats induced by AHSFD,AHFD,HSFD and alcohol can raise BP obviously with underlying hepatic and kidney damage,the lipid profiles disorder and TCM symptoms change,while the AH(S)FD-induced hypertension models are earlier and more stable.RPA.E could mildly reduce BP level,and improve the lipid profiles disorders,hepatic damage,which reflects the characteristics of TCM on antihypertensive effects.The above models have their own characteristics,can be used to study the causes and pathogenesis of hypertension complicating metabolic disorder and the related treatment drug screening.
基金This work was supported by a Longer and Larger(LoLa)grant from the Biotechnology and Biological Sciences Research Council(BBSRC,grant numbers BB/G020744/1,BB/G019177/1,BB/G019274/1 and BB/G018553/1)the UK Department for Environment,Food and Rural Affairs and Zoetis awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology(BRaDP1T)consortium.Funding for LZ was provided by the BBSRC(grant number BB/C508193/1)The funders had no role in study design,data collection and analysis,decision to publish,or preparation of the manuscript.
文摘Comprehensive identification of conditionally essential genes requires efficient tools for generating high-density transposon libraries that, ideally, can be analysed using next-generation sequencing methods such as Transposon Directed Insertion-site Sequencing (TraDIS). The Himar1 (mariner) transposon is ideal for generating near-saturating mutant libraries, especially in AT-rich chromosomes, as the requirement for integration is a TA dinucleotide, and this transposon has been used for mutagenesis of a wide variety of bacteria. However, plasmids for mariner delivery do not necessarily work well in all bacteria. In particular, there are limited tools for functional genomic analysis of Pasteurellaceae species of major veterinary importance, such as swine and cattle pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida, respectively. Here, we developed plasmids, pTsodCPC9 and pTlacPC9 (differing only in the promoter driving expression of the transposase gene), that allow delivery of mariner into both these pathogens, but which should also be applicable to a wider range of bacteria. Using the pTlacPC9 vector, we have generated, for the first time, saturating mariner mutant libraries in both A. pleuropneumoniae and P. multocida that showed a near random distribution of insertions around the respective chromosomes as detected by TraDIS. A preliminary screen of 5000 mutants each identified 8 and 14 genes, respectively, that are required for growth under anaerobic conditions. Future high-throughput screening of the generated libraries will facilitate identification of mutants required for growth under different conditions, including in vivo, highlighting key virulence factors and pathways that can be exploited for development of novel therapeutics and vaccines.
文摘目的探讨活血化瘀中药熏药对乳腺癌改良根治术后切口愈合的影响。方法选取2022年12月至2024年12月赣南医科大学第二附属医院收治的60例行乳腺癌改良根治术治疗的乳腺癌患者作为研究对象,按照随机数字表法分为对照组与观察组,每组30例。对照组术后给予常规消毒及正常换药,观察组在对照组的基础上联合活血化瘀中药熏药,比较两组患者切口愈合能力(切口愈合初期、中期及完全愈合所需时间),术后疼痛程度[术后第1、4天视觉模拟评分法(VAS)评分],术前及术后炎症水平[术前1 d及术后4 d降钙素原(PCT)、C反应蛋白(CRP)]及术后治疗效果。结果两组患者术前炎症水平、术后切口愈合初期所需时间及术后第1天VAS评分比较,差异无统计学意义(P>0.05)。观察组术后切口愈合中期及完全愈合所需时间均短于对照组,术后第4天VAS评分低于对照组,术后4 d PCT、CRP水平均低于对照组,治疗总有效率高于对照组,差异有统计学意义(P<0.05)。结论活血化瘀中药熏药可有效促进乳腺癌患者改良根治术后的切口愈合,缓解患者疼痛程度,改善炎症反应,疗效显著。
