Nonhuman primates are increasingly being used as animal models in neuroscience research.However,efficient neuronal tracing techniques for labeling motor neurons and primary sensory afferents in the monkey spinal cord ...Nonhuman primates are increasingly being used as animal models in neuroscience research.However,efficient neuronal tracing techniques for labeling motor neurons and primary sensory afferents in the monkey spinal cord are lacking.Here,by injecting the cholera toxin B subunit into the sciatic nerve of a rhesus monkey,we successfully labeled the motor neurons and primary sensory afferents in the lumbar and sacralspinal cord.Labeled alpha motor neurons were located in lamina IX of the L6–S1 segments,which innervate both flexors and extensors.The labeled primary sensory afferents were mainly myelinated Aβfibers that terminated mostly in laminae I and II of the L4–L7 segments.Together with the labeled proprioceptive afferents,the primary sensory afferents formed excitatory synapses with multiple types of spinal neurons.In summary,our methods successfully traced neuronal connections in the monkey spinal cord and can be used in spinal cord studies when nonhuman primates are used.展开更多
In the field of intelligent education,the integration of artificial intelligence,especially deep learning technologies,has garnered significant attention.Knowledge tracing(KT)plays a pivotal role in this field by pred...In the field of intelligent education,the integration of artificial intelligence,especially deep learning technologies,has garnered significant attention.Knowledge tracing(KT)plays a pivotal role in this field by predicting students’future performance through the analysis of historical interaction data,thereby assisting educators in evaluating knowledgemastery and tailoring instructional strategies.Traditional knowledge tracingmethods,largely based on Recurrent Neural Networks(RNNs)and Transformer models,primarily focus on capturing long-term interaction patterns in sequential data.However,these models may neglect crucial short-term dynamics and other relevant features.This paper introduces a novel approach to knowledge tracing by leveraging a pure Multilayer Perceptron(MLP)architecture.We proposeMixerKT,a knowledge tracing model based on theHyperMixer framework,which uniquely integrates global and localMixer feature extractors.This architecture enables more effective extraction of both long-terminteraction trends and recent learning behaviors,addressing limitations in currentmodels thatmay overlook these key aspects.Empirical evaluations on twowidely-used datasets,ASSIS Tments2009 and Algebra2005,demonstrate that MixerKT consistently outperforms several state-of-the-art models,including DKT,SAKT,and Separated Self-Attentive Neural Knowledge Tracing(SAINT).Specifically,MixerKT achieves higher prediction accuracy,highlighting its effectiveness in capturing the nuances of learners’knowledge states.These results indicate that our model provides a more comprehensive representation of student learning patterns,enhancing the ability to predict future performance with greater precision.展开更多
Although ray tracing produces high-fidelity, realistic images, it is considered computationally burdensome when implemented on a high rendering rate system. Perception-driven rendering techniques generate images with ...Although ray tracing produces high-fidelity, realistic images, it is considered computationally burdensome when implemented on a high rendering rate system. Perception-driven rendering techniques generate images with minimal noise and distortion that are generally acceptable to the human visual system, thereby reducing rendering costs. In this paper, we introduce a perception-entropy-driven temporal reusing method to accelerate real-time ray tracing. We first build a just noticeable difference(JND) model to represent the uncertainty of ray samples and image space masking effects. Then, we expand the shading gradient through gradient max-pooling and gradient filtering to enlarge the visual receipt field. Finally, we dynamically optimize reusable time segments to improve the accuracy of temporal reusing. Compared with Monte Carlo ray tracing, our algorithm enhances frames per second(fps) by 1.93× to 2.96× at 8 to 16 samples per pixel, significantly accelerating the Monte Carlo ray tracing process while maintaining visual quality.展开更多
The art of polychrome lacquer painting with gold tracing and inlay represents the culmination of traditional Chinese lacquer craftsmanship.It integrates four core techniques-lacquer application,polychrome painting,gol...The art of polychrome lacquer painting with gold tracing and inlay represents the culmination of traditional Chinese lacquer craftsmanship.It integrates four core techniques-lacquer application,polychrome painting,gold tracing,and inlay-embodying the pinnacle of ancient artisan ingenuity.This paper begins by outlining the historical origins,technical characteristics,and cultural significance of this art form.Subsequently,using a Qing Dynasty“Hanging Screen Inlaid with Boxwood and Coral Depicting the Scene of Sudhana Paying Homage to Guanyin,with Polychrome Lacquer Painting and Gold Tracing”as a case study,it offers an in-depth analysis of its craftsmanship,visual elements,thematic narrative,and cultural connotations.The study aims to unveil its artistic value and contemporary relevance as a form of“living cultural heritage.”展开更多
The opening of the Central South Atlantic and the consequent formation of the eastern Brazilian continental margin was marked by a complex history of mafic magmatism,carbonate sedimentation,and deposition of a thick s...The opening of the Central South Atlantic and the consequent formation of the eastern Brazilian continental margin was marked by a complex history of mafic magmatism,carbonate sedimentation,and deposition of a thick salt layer.The carbonates underlying the salt layer(pre-salt carbonates)were formed in restricted lacustrine basins.Here,the timing and fluid sources of deposition,diagenetic,and hydrothermal alterations of the pre-salt carbonate rocks are defined through in-situ U-Pb dating,87Sr/86Sr,and trace element analyses of samples from the Santos Basin.The very alkaline nature of the Aptian lake(s)produced characteristically unique and widely distributed carbonate rocks such as Mg-clays with calcite spherulite and calcite crystal shrub limestones transitioning laterally and vertically into travertines formed by hydrothermal pulses during basin evolution.Hydrothermalism caused extensive replacement,dissolution,and calcite cementation.REE+Y PAAS-normalised patterns and 87Sr/86Sr ratios indicate that deposition/eo-diagenesis of the primary carbonates occurred in a lacustrine environment primarily controlled by evaporation,pH,and continental water source,with 2%–10%hydrothermal fluid input.Trace elements and Sr-isotope of travertines and burial diagenetic phases show that they are produced from a hot mixture of mafic/mantle-derived fluids and dissolution/alteration of older carbonate formations.U-Pb dating indicates that carbonate deposition occurred between 124.8±2.6 Ma and 120.0±1.6 Ma,earlier than previously proposed,followed closely by the circulation of hydrothermal fluids.Replacement and cementation ages range from 120.5±2.4 Ma to 80.4±2.4 Ma.展开更多
Shallow-water seabed reverberation presents a critical disturbance in acoustic propagation,affecting the target detection performance of monostatic sonar.This paper proposes a novel seabed reverberation model integrat...Shallow-water seabed reverberation presents a critical disturbance in acoustic propagation,affecting the target detection performance of monostatic sonar.This paper proposes a novel seabed reverberation model integrating Gaussian beam tracing with seabed scattering physics.The model synthesizes time-domain reverberation signals by superimposing scattering signals received across multiple propagation paths.It accurately resolves scattering signals along distinct paths and enables simulation of reverberation under diverse shallow-water environments by adjusting the marine parameters.Furthermore,we model the seabed reverberation signals in the time domain and the space domain for a cylindrical transceiver array,and provide a detailed statistical characterization of the simulated seabed reverberation signals.Finally,shallow-water seabed reverberation experiments were conducted with a cylindrical transceiver array.Comparisons between shallow-water seabed reverberation measurements and simulation estimates at various sites and transceiver depths demonstrate that the proposed seabed reverberation model can efficiently simulate shallow-water seabed reverberation.展开更多
Background:Rabies virus(RABV)-derived neuronal tracing tools are extensively applied in retrograde tracing due to their strict retrograde transsynaptic transfer property and low neurotoxicity.However,the RABV infectio...Background:Rabies virus(RABV)-derived neuronal tracing tools are extensively applied in retrograde tracing due to their strict retrograde transsynaptic transfer property and low neurotoxicity.However,the RABV infection and expression of fluorescence products would be gradually cleared while the infected neurons still survive,a phenomenon known as non-cytolytic immune clearance(NCLIC).This phenomenon introduced the risk of fluorescence loss and led to the omission of a subset of neurons that should be labeled,thereby interfering in the analysis of tracing results.Methods:To compensate for the fluorescence loss problem,in this study,we developed a novel marker footprints(MF)mouse,involving a Cre recombinase-dependent red fluorescent reporter system and systemic expression of glycoprotein(G)and ASLV-A receptor(TVA).Using this mouse model combined with the well-developed RABV-EnvA-ΔG-GFP-Cre viral tool,we developed a novel green-to-red spectral labeling strategy.Results:Neurons in the MF mouse could be co-labeled with green fluorescence from the very quick expression of the viral tool and with red fluorescence from the relatively slow expression of the neuron itself,so neurons undergoing NCLIC with green fluorescence loss could be relabeled red.Furthermore,newly infected neurons could be labeled green and other neurons could be labeled yellow due to the temporal expression difference between the two fluorescent proteins.Conclusions:This is the first polysynaptic retrograde tracing labeling strategy that could label neurons using spectral fluorescence colors with only one injection of the viral tool,enabling its application in recognizing the labeling sequence of neurons in brain regions and enhancing the spatiotemporal resolution of neuronal tracing.展开更多
Cell lineage tracing is a key technology for describing the developmental history of individual progenitor cells and assembling them to form a lineage development tree.However,traditional methods have limitations of p...Cell lineage tracing is a key technology for describing the developmental history of individual progenitor cells and assembling them to form a lineage development tree.However,traditional methods have limitations of poor stability and insufficient reso-lution.As an efficient and flexible gene editing tool,CRISPR-Cas9 system has been widely used in biological research.Furthermore,CRISPR-Cas9 gene editing-based tracing methods can introduce fluorescent proteins,reporter genes,or DNA barcodes for high-throughput sequencing,enabling precise lineage analysis,significantly im-proving precision and resolution,and expanding its application range.In this review,we summarize applications of CRISPR-Cas9 system in cell lineage tracing,with special emphasis on its successful applications in traditional model animals(e.g.,zebrafish and mice),large animal models(pigs),and human cells or organoids.We also discussed its potential prospects and challenges in xenotransplantation and regenerative medicine.展开更多
To address the problem of subdividing inflexible rectangular grid models and their poor definition of velocity interfaces,we propose a complex structure triangular net for a minimum traveltime ray tracing global algor...To address the problem of subdividing inflexible rectangular grid models and their poor definition of velocity interfaces,we propose a complex structure triangular net for a minimum traveltime ray tracing global algorithm.Our procedure is：（1） Subdivide a triangle grid based on the Delaunay triangular subdivision criterion and the relationships of the points,lines,and the surfaces in the subdividing area.（2） Define the topology relationships and related concepts of triangular unit ray tracing.（3） The source point and wave arrival points at any time compose the propagating plane wave and the minimum traveltime and secondary source positions are calculated during the plane wave propagation.We adopt the hyperbolic approximation global algorithm for secondary source retrieving.（4） By minimum traveltime ray tracing,collect the path from receiver to source points with the neighborhood point＇s traveltime and the direction of the secondary source.Numerical simulation examples are given to test the algorithm.The results show that the triangular net ray tracing method demonstrates model subdivision flexibility,precise velocity discontinuity interfaces,and accurate computations.展开更多
A fast algorithm for ray tracing is presented, with which the specular reflection term of global illumination model is improved. A hybrid technique combining hierarchical bounding volumes and constant size box partiti...A fast algorithm for ray tracing is presented, with which the specular reflection term of global illumination model is improved. A hybrid technique combining hierarchical bounding volumes and constant size box partitioning is presented and a fast box traversal algorithm is used. By this technique multiple ray intersections with objects that are in more than one box can be avoided. As a result, the speed of ray tracing is considerably increased.展开更多
To perform various functions in the body,skeletal muscle is controlled and coordinated as a whole by nerves.However,there has been little research into whether the nerve control characteristics of different muscles ar...To perform various functions in the body,skeletal muscle is controlled and coordinated as a whole by nerves.However,there has been little research into whether the nerve control characteristics of different muscles are different,and the importance of these potential differences.In the present study,we used a three-dimensional imaging of solvent-cleared organ-compatible multi-tracer technique to explore the spatial distribution patterns of sensory and sympathetic neurons that innervate limb muscles.We integrated transcriptome sequencing datasets from mouse limb muscles in public databases and performed correlation analysis with neuronal spatial distribution data to reveal the unique effects of different types of neurons on muscle functional pathways.In terms of spatial distribution patterns,sympathetic neurons exhibited a more concentrated distribution than sensory and motor neurons.In addition,the neuronal innervation of limb muscles exhibited four different characteristics:sympathetic neuron-rich muscle,sensory neuron-rich muscle,neuron-sparse muscle,and motor neuron-rich muscle.Sensory neuron density was mainly associated with muscle contractile structure and cell pH,whereas sympathetic neuron density was associated with protein kinase activity,muscle vasculature,muscle calcium-dependent protein kinase activity,lipid transport,and vesicle release.Motor neuron density was mainly associated with protein kinase activity,cell adhesion,oxidoreductase activity,and exocytosis.These findings may contribute to a deeper understanding of how nerves cooperate to endow muscles with diverse physiological functions,thereby providing new insights and experimental evidence for the treatment of various neuromuscular diseases.展开更多
Exogenous neural stem cell transplantation has become one of the most promising treatment methods for chronic stroke.Recent studies have shown that most ischemia-reperfusion model rats recover spontaneously after inju...Exogenous neural stem cell transplantation has become one of the most promising treatment methods for chronic stroke.Recent studies have shown that most ischemia-reperfusion model rats recover spontaneously after injury,which limits the ability to observe long-term behavioral recovery.Here,we used a severe stroke rat model with 150 minutes of ischemia,which produced severe behavioral deficiencies that persisted at 12 weeks,to study the therapeutic effect of neural stem cells on neural restoration in chronic stroke.Our study showed that stroke model rats treated with human neural stem cells had long-term sustained recovery of motor function,reduced infarction volume,long-term human neural stem cell survival,and improved local inflammatory environment and angiogenesis.We also demonstrated that transplanted human neural stem cells differentiated into mature neurons in vivo,formed stable functional synaptic connections with host neurons,and exhibited the electrophysiological properties of functional mature neurons,indicating that they replaced the damaged host neurons.The findings showed that human fetal-derived neural stem cells had long-term effects for neurological recovery in a model of severe stroke,which suggests that human neural stem cells-based therapy may be effective for repairing damaged neural circuits in stroke patients.展开更多
Nitrogen use efficiency in rice is lower than in upland crops,likely due to differences in soil nitrogen dynamics and crop nitrogen preferences.However,the specific nitrogen dynamics in paddy and upland systems and th...Nitrogen use efficiency in rice is lower than in upland crops,likely due to differences in soil nitrogen dynamics and crop nitrogen preferences.However,the specific nitrogen dynamics in paddy and upland systems and their impact on crop nitrogen uptake remain poorly understood.The N dynamics and impact on crop N uptake determine the downstream environmental pollution from nitrogen fertilizer.To address this poor understanding,we analyzed 2,044 observations of gross nitrogen transformation rates in soils from 136 studies to examine nitrogen dynamics in both systems and their effects on nitrogen uptake in rice and upland crops.Our findings revealed that nitrogen mineralization and autotrophic nitrification rates are lower in paddies than in upland soil,while dissimilatory nitrate reduction to ammonium is higher in paddies,these differences being driven by flooding and lower total nitrogen content in paddies.Rice exhibited higher ammonium uptake,while upland crops had over twice the nitrate uptake.Autotrophic nitrification stimulated by p H reduced rice nitrogen uptake,while heterotrophic nitrification enhanced nitrogen uptake of upland crops.Autotrophic nitrification played a key role in regulating the ammonium-to-nitrate ratio in soils,which further affected the balance of plant nitrogen uptake.These results highlight the need to align soil nitrogen dynamics with crop nitrogen preferences to maximize plant maximize productivity and reduce reactive nitrogen pollution.展开更多
Background:Excessive use of inorganic trace minerals(ITMs)in swine production leads to high fecal mineral excretion and environmental risks,while most studies on organic trace minerals(OTMs)focus on single elements,wi...Background:Excessive use of inorganic trace minerals(ITMs)in swine production leads to high fecal mineral excretion and environmental risks,while most studies on organic trace minerals(OTMs)focus on single elements,with limited data on the synergistic effects and molecular mechanisms of combined OTMs(Fe,Cu,Mn,Zn)in growing-finishing pigs.Methods:This study aimed to investigate the effects of graded levels of micromineral proteinates(combined OTMs)on growth performance,mineral metabolism,and mRNA expression of mineral regulatory proteins.A total of 360 crossbred Duroc×Landrace×Large White pigs(initial body weight 47.1±4.8 kg)were randomly assigned to 6 dietary treatments:basal diet without microminerals(CON),basal diet with ITMs at commercially recommended levels(IT),and basal diets with 15%(OT 15%),25%(OT 25%),35%(OT 35%)commercially recommended levels(CRL)of combined micromineral proteinates.After a 70-day feeding trial,samples were analyzed using ICP-OES,ELISA,and RT-qPCR.Results:Results showed that reduced levels(15-35%CRL)of micromineral proteinates did not significantly affect average daily gain,average daily feed intake,or feed conversion ratio(gain-to-feed ratio)compared to IT(P>0.05),but significantly increased plasma Cu(1.73-1.83μg/mL)and Zn(1.72-1.97μg/mL)concentrations(P<0.05)and elevated activities of Cu/Zn-superoxide dismutase(32.9-35.9 U/L)and manganese superoxide dismutase(20.5-24.1 U/L)compared to CON(P<0.05),with no significant differences from IT(P>0.05).Fecal excretion of Fe,Cu,Mn,and Zn was significantly reduced by 35-50%in OT 15%-OT 35%groups compared to IT(P<0.05).OT 25%group exhibited the highest apparent absorptivity of Fe(38.5%),Cu(27.8%),and Zn(42.4%)(P<0.05),which was associated with significantly regulated mRNA expression of mineral regulatory proteins:upregulated DMT1,FPN1,ZIP4,and MT1A in the duodenum,and modulated HAMP,ATP7B,ZIP14,and ZnT1 in the liver(P<0.05).Conclusion:In conclusion,dietary supplementation with 25%CRL or less of combined micromineral proteinates can fully meet the nutritional needs of growing-finishing pigs,improve mineral absorptivity,and reduce fecal mineral excretion by regulating intestinal and hepatic mineral transport and homeostatic proteins,providing a sustainable alternative to high-dose ITMs.展开更多
In Brazil, the use of software and applications in railway geometric designs is rare. Currently, after the division and privatization of the national rail network, the concessionaires who assumed the administration, a...In Brazil, the use of software and applications in railway geometric designs is rare. Currently, after the division and privatization of the national rail network, the concessionaires who assumed the administration, are investing in modernization, recovery and extension of this transport model to make it more efficient. The PIL （Program of Investment in Logistics） has generated growth of the sector in response to the amount of investments, greatly enhancing the load capacity and optimizing the geometric design, which would allow speed of 80 km/h. Based on this information, the objective of this research is to verify the software applications of Power Rail Track Program of Bentley Systems, Inc. for geometric designs in future deployments of railways in Brazil. This software is based on numerical calculations, including roller adjustment by the least squares method to calculate and choose the best alignments, and regression tools, to a fast and effectively optimization of various parameters of the geometry for a future or existing railway. Different variables that allow you to optimize the geometric design are analyzed, considering several optional alignments and complex restrictions present in geometric design, taking into account variables such as circular curves, transition, tangents and rays. The software regression tools include curvature diagrams identifying points and curves, tangent and spirals. The analysis results are presented quickly and points can be added or deleted from the regression analysis, providing improved tracing. The software also allows to detail the elements of the executive project of the railway line such as rails, joints, turning points, waypoints and others. The result of this study has shown that the application of the Power Rail Track Program generates significant cost savings in time and increases in productivity, providing greater opportunity to optimize the geometry and reducing geometric design costs.展开更多
Automated Program Repair(APR)techniques have shown significant potential in mitigating the cost and complexity associated with debugging by automatically generating corrective patches for software defects.Despite cons...Automated Program Repair(APR)techniques have shown significant potential in mitigating the cost and complexity associated with debugging by automatically generating corrective patches for software defects.Despite considerable progress in APR methodologies,existing approaches frequently lack contextual awareness of runtime behaviors and structural intricacies inherent in buggy source code.In this paper,we propose a novel APR approach that integrates attention mechanisms within an autoencoder-based framework,explicitly utilizing structural code affinity and execution context correlation derived from stack trace analysis.Our approach begins with an innovative preprocessing pipeline,where code segments and stack traces are transformed into tokenized representations.Subsequently,the BM25 ranking algorithm is employed to quantitatively measure structural code affinity and execution context correlation,identifying syntactically and semantically analogous buggy code snippets and relevant runtime error contexts from extensive repositories.These extracted features are then encoded via an attention-enhanced autoencoder model,specifically designed to capture significant patterns and correlations essential for effective patch generation.To assess the efficacy and generalizability of our proposed method,we conducted rigorous experimental comparisons against DeepFix,a state-of-the-art APR system,using a substantial dataset comprising 53,478 studentdeveloped C programs.Experimental outcomes indicate that our model achieves a notable bug repair success rate of approximately 62.36%,representing a statistically significant performance improvement of over 6%compared to the baseline.Furthermore,a thorough K-fold cross-validation reinforced the consistency,robustness,and reliability of our method across diverse subsets of the dataset.Our findings present the critical advantage of integrating attentionbased learning with code structural and execution context features in APR tasks,leading to improved accuracy and practical applicability.Future work aims to extend the model’s applicability across different programming languages,systematically optimize hyperparameters,and explore alternative feature representation methods to further enhance debugging efficiency and effectiveness.展开更多
As the chemical industry expands,the use of benzene,toluene,and xylene(collectively known as BTX)in industrial production has increased greatly.Meanwhile,the toxic nature and potential health hazards of BTX gases cann...As the chemical industry expands,the use of benzene,toluene,and xylene(collectively known as BTX)in industrial production has increased greatly.Meanwhile,the toxic nature and potential health hazards of BTX gases cannot be ignored due to low-concentration leaks underline the critical need for rapid and real-time monitoring of these gases.Chemiresistive metal oxide semiconductor(MOS)-based gas sensors,which are extensively used for gas detection in both industrial settings and everyday life,emerge as one of the optimal solutions for trace BTX detection.These sensors are highly valued for their high sensitivity and low detection limits.Nevertheless,the improvement of selectivity towards specific BTX gases to achieve efficient and precise detection still remains challenging.This review summarizes the chemiresistive MOS-based gas sensors designed for BTX detection,categorizing them based on the components of sensing materials-basically into three groups:single-component,single heterojunction,and multiple heterojunctions gas sensing materials.Further,the review proposes the future application prospects of chemiresistive MOS-based BTX gas sensors,with specific emphasis on their significance in promoting industrial safety and environmental monitoring.展开更多
A central objective in deciphering the nervous system in health and disease is to define the connections of neurons. The propensity of neurotropic viruses to spread among synaptically-linked neurons makes them ideal f...A central objective in deciphering the nervous system in health and disease is to define the connections of neurons. The propensity of neurotropic viruses to spread among synaptically-linked neurons makes them ideal for mapping neural circuits. So far, several classes of viral neuronal tracers have become available and provide a powerful toolbox for delineating neural networks. In this paper, we review the recent developments of neurotropic viral tracers and highlight their unique properties in revealing patterns of neuronal connections.展开更多
基金supported by a grant from Ministry of Science and Technology China,No.2022ZD0204704(to WW)the National Natural Science Foundation of China,No.82301572(to XZ)the China Postdoctoral Science Foundation,No.2023M731202(to XZ)。
文摘Nonhuman primates are increasingly being used as animal models in neuroscience research.However,efficient neuronal tracing techniques for labeling motor neurons and primary sensory afferents in the monkey spinal cord are lacking.Here,by injecting the cholera toxin B subunit into the sciatic nerve of a rhesus monkey,we successfully labeled the motor neurons and primary sensory afferents in the lumbar and sacralspinal cord.Labeled alpha motor neurons were located in lamina IX of the L6–S1 segments,which innervate both flexors and extensors.The labeled primary sensory afferents were mainly myelinated Aβfibers that terminated mostly in laminae I and II of the L4–L7 segments.Together with the labeled proprioceptive afferents,the primary sensory afferents formed excitatory synapses with multiple types of spinal neurons.In summary,our methods successfully traced neuronal connections in the monkey spinal cord and can be used in spinal cord studies when nonhuman primates are used.
基金supported by National Natural Science Foundation of China(Nos.62266054 and 62166050)Key Program of Fundamental Research Project of Yunnan Science and Technology Plan(No.202201AS070021)+2 种基金Yunnan Fundamental Research Projects(No.202401AT070122)Yunnan International Joint Research and Development Center of China-Laos-Thailand Educational Digitalization(No.202203AP140006)Scientific Research Foundation of Yunnan Provincial Department of Education(No.2024Y159).
文摘In the field of intelligent education,the integration of artificial intelligence,especially deep learning technologies,has garnered significant attention.Knowledge tracing(KT)plays a pivotal role in this field by predicting students’future performance through the analysis of historical interaction data,thereby assisting educators in evaluating knowledgemastery and tailoring instructional strategies.Traditional knowledge tracingmethods,largely based on Recurrent Neural Networks(RNNs)and Transformer models,primarily focus on capturing long-term interaction patterns in sequential data.However,these models may neglect crucial short-term dynamics and other relevant features.This paper introduces a novel approach to knowledge tracing by leveraging a pure Multilayer Perceptron(MLP)architecture.We proposeMixerKT,a knowledge tracing model based on theHyperMixer framework,which uniquely integrates global and localMixer feature extractors.This architecture enables more effective extraction of both long-terminteraction trends and recent learning behaviors,addressing limitations in currentmodels thatmay overlook these key aspects.Empirical evaluations on twowidely-used datasets,ASSIS Tments2009 and Algebra2005,demonstrate that MixerKT consistently outperforms several state-of-the-art models,including DKT,SAKT,and Separated Self-Attentive Neural Knowledge Tracing(SAINT).Specifically,MixerKT achieves higher prediction accuracy,highlighting its effectiveness in capturing the nuances of learners’knowledge states.These results indicate that our model provides a more comprehensive representation of student learning patterns,enhancing the ability to predict future performance with greater precision.
基金supported by the National Natural Science Foundation of China (No.U19A2063)the Jilin Provincial Science&Technology Development Program of China (No.20230201080GX)。
文摘Although ray tracing produces high-fidelity, realistic images, it is considered computationally burdensome when implemented on a high rendering rate system. Perception-driven rendering techniques generate images with minimal noise and distortion that are generally acceptable to the human visual system, thereby reducing rendering costs. In this paper, we introduce a perception-entropy-driven temporal reusing method to accelerate real-time ray tracing. We first build a just noticeable difference(JND) model to represent the uncertainty of ray samples and image space masking effects. Then, we expand the shading gradient through gradient max-pooling and gradient filtering to enlarge the visual receipt field. Finally, we dynamically optimize reusable time segments to improve the accuracy of temporal reusing. Compared with Monte Carlo ray tracing, our algorithm enhances frames per second(fps) by 1.93× to 2.96× at 8 to 16 samples per pixel, significantly accelerating the Monte Carlo ray tracing process while maintaining visual quality.
文摘The art of polychrome lacquer painting with gold tracing and inlay represents the culmination of traditional Chinese lacquer craftsmanship.It integrates four core techniques-lacquer application,polychrome painting,gold tracing,and inlay-embodying the pinnacle of ancient artisan ingenuity.This paper begins by outlining the historical origins,technical characteristics,and cultural significance of this art form.Subsequently,using a Qing Dynasty“Hanging Screen Inlaid with Boxwood and Coral Depicting the Scene of Sudhana Paying Homage to Guanyin,with Polychrome Lacquer Painting and Gold Tracing”as a case study,it offers an in-depth analysis of its craftsmanship,visual elements,thematic narrative,and cultural connotations.The study aims to unveil its artistic value and contemporary relevance as a form of“living cultural heritage.”
基金C.Lana and F.F.Alkmim benefit from the Brazilian National Research Council(CNPq)research grants 3073353/2019-2 and 311543/2020-0.C.Lana,F.F.Alkmim,and Maria Eugênia Souza are part of the Instituto GeoAtlântico,a National Institute of Science and Technology,CNPq-Brazil process 405653/2022-0We acknowledge Petrobras’s support during the investigation and permission to publish this study.I.Figueiredo acknowledged the Brazilian Federal Agency for Support and Evaluation of Graduate Education(CAPES)for Master's scholarship 88887.816343/2023-00.
文摘The opening of the Central South Atlantic and the consequent formation of the eastern Brazilian continental margin was marked by a complex history of mafic magmatism,carbonate sedimentation,and deposition of a thick salt layer.The carbonates underlying the salt layer(pre-salt carbonates)were formed in restricted lacustrine basins.Here,the timing and fluid sources of deposition,diagenetic,and hydrothermal alterations of the pre-salt carbonate rocks are defined through in-situ U-Pb dating,87Sr/86Sr,and trace element analyses of samples from the Santos Basin.The very alkaline nature of the Aptian lake(s)produced characteristically unique and widely distributed carbonate rocks such as Mg-clays with calcite spherulite and calcite crystal shrub limestones transitioning laterally and vertically into travertines formed by hydrothermal pulses during basin evolution.Hydrothermalism caused extensive replacement,dissolution,and calcite cementation.REE+Y PAAS-normalised patterns and 87Sr/86Sr ratios indicate that deposition/eo-diagenesis of the primary carbonates occurred in a lacustrine environment primarily controlled by evaporation,pH,and continental water source,with 2%–10%hydrothermal fluid input.Trace elements and Sr-isotope of travertines and burial diagenetic phases show that they are produced from a hot mixture of mafic/mantle-derived fluids and dissolution/alteration of older carbonate formations.U-Pb dating indicates that carbonate deposition occurred between 124.8±2.6 Ma and 120.0±1.6 Ma,earlier than previously proposed,followed closely by the circulation of hydrothermal fluids.Replacement and cementation ages range from 120.5±2.4 Ma to 80.4±2.4 Ma.
基金The Youth Project of National Natural Science Foundation of China under contract No.12304507the Natural Science Foundation of Guangdong Province,China under contract No.2024A1515011512+2 种基金the Stable Supporting Fund of Acoustic Science and Technology Laboratory under contract No.JCKYS2025SSJS010the Fundamental Research Funds for the Central Universities under contract No.20720240108the Special Project for Marine Economy Development of Guangdong Province,China under contract No.GDNRC2023-47.
文摘Shallow-water seabed reverberation presents a critical disturbance in acoustic propagation,affecting the target detection performance of monostatic sonar.This paper proposes a novel seabed reverberation model integrating Gaussian beam tracing with seabed scattering physics.The model synthesizes time-domain reverberation signals by superimposing scattering signals received across multiple propagation paths.It accurately resolves scattering signals along distinct paths and enables simulation of reverberation under diverse shallow-water environments by adjusting the marine parameters.Furthermore,we model the seabed reverberation signals in the time domain and the space domain for a cylindrical transceiver array,and provide a detailed statistical characterization of the simulated seabed reverberation signals.Finally,shallow-water seabed reverberation experiments were conducted with a cylindrical transceiver array.Comparisons between shallow-water seabed reverberation measurements and simulation estimates at various sites and transceiver depths demonstrate that the proposed seabed reverberation model can efficiently simulate shallow-water seabed reverberation.
基金Hubei Natural Science Foundation of China,Grant/Award Number:2024AFB593。
文摘Background:Rabies virus(RABV)-derived neuronal tracing tools are extensively applied in retrograde tracing due to their strict retrograde transsynaptic transfer property and low neurotoxicity.However,the RABV infection and expression of fluorescence products would be gradually cleared while the infected neurons still survive,a phenomenon known as non-cytolytic immune clearance(NCLIC).This phenomenon introduced the risk of fluorescence loss and led to the omission of a subset of neurons that should be labeled,thereby interfering in the analysis of tracing results.Methods:To compensate for the fluorescence loss problem,in this study,we developed a novel marker footprints(MF)mouse,involving a Cre recombinase-dependent red fluorescent reporter system and systemic expression of glycoprotein(G)and ASLV-A receptor(TVA).Using this mouse model combined with the well-developed RABV-EnvA-ΔG-GFP-Cre viral tool,we developed a novel green-to-red spectral labeling strategy.Results:Neurons in the MF mouse could be co-labeled with green fluorescence from the very quick expression of the viral tool and with red fluorescence from the relatively slow expression of the neuron itself,so neurons undergoing NCLIC with green fluorescence loss could be relabeled red.Furthermore,newly infected neurons could be labeled green and other neurons could be labeled yellow due to the temporal expression difference between the two fluorescent proteins.Conclusions:This is the first polysynaptic retrograde tracing labeling strategy that could label neurons using spectral fluorescence colors with only one injection of the viral tool,enabling its application in recognizing the labeling sequence of neurons in brain regions and enhancing the spatiotemporal resolution of neuronal tracing.
基金supported by Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences and Comparative Medicine Center,Peking Union Medical College,Collaborative Innovation Program of the Chinese Academy of Sciences(22SH19)Nonprofit Central Research Institute Fund of Chinese Academy of Medical Sciences(2023-PT180-01).
文摘Cell lineage tracing is a key technology for describing the developmental history of individual progenitor cells and assembling them to form a lineage development tree.However,traditional methods have limitations of poor stability and insufficient reso-lution.As an efficient and flexible gene editing tool,CRISPR-Cas9 system has been widely used in biological research.Furthermore,CRISPR-Cas9 gene editing-based tracing methods can introduce fluorescent proteins,reporter genes,or DNA barcodes for high-throughput sequencing,enabling precise lineage analysis,significantly im-proving precision and resolution,and expanding its application range.In this review,we summarize applications of CRISPR-Cas9 system in cell lineage tracing,with special emphasis on its successful applications in traditional model animals(e.g.,zebrafish and mice),large animal models(pigs),and human cells or organoids.We also discussed its potential prospects and challenges in xenotransplantation and regenerative medicine.
基金the National Natural Science Foundation of China(Grant Nos.50974081,50774051, 51034003)the Foundation for the Author of National Excellent Doctoral Dissertation of PR China(Grant No.200958)+1 种基金the Program for Changjiang Scholars and Innovative Research Team in University(Grant No.IRT0843)the Open Research Fund Program of the State Key Laboratory of Coal Resources and Safe Mining(China University of Mining and Technology, Beijing)(No.2007-07)
文摘To address the problem of subdividing inflexible rectangular grid models and their poor definition of velocity interfaces,we propose a complex structure triangular net for a minimum traveltime ray tracing global algorithm.Our procedure is：（1） Subdivide a triangle grid based on the Delaunay triangular subdivision criterion and the relationships of the points,lines,and the surfaces in the subdividing area.（2） Define the topology relationships and related concepts of triangular unit ray tracing.（3） The source point and wave arrival points at any time compose the propagating plane wave and the minimum traveltime and secondary source positions are calculated during the plane wave propagation.We adopt the hyperbolic approximation global algorithm for secondary source retrieving.（4） By minimum traveltime ray tracing,collect the path from receiver to source points with the neighborhood point＇s traveltime and the direction of the secondary source.Numerical simulation examples are given to test the algorithm.The results show that the triangular net ray tracing method demonstrates model subdivision flexibility,precise velocity discontinuity interfaces,and accurate computations.
文摘A fast algorithm for ray tracing is presented, with which the specular reflection term of global illumination model is improved. A hybrid technique combining hierarchical bounding volumes and constant size box partitioning is presented and a fast box traversal algorithm is used. By this technique multiple ray intersections with objects that are in more than one box can be avoided. As a result, the speed of ray tracing is considerably increased.
基金supported by the National Natural Science Foundation of China,No.82072162(to XY).
文摘To perform various functions in the body,skeletal muscle is controlled and coordinated as a whole by nerves.However,there has been little research into whether the nerve control characteristics of different muscles are different,and the importance of these potential differences.In the present study,we used a three-dimensional imaging of solvent-cleared organ-compatible multi-tracer technique to explore the spatial distribution patterns of sensory and sympathetic neurons that innervate limb muscles.We integrated transcriptome sequencing datasets from mouse limb muscles in public databases and performed correlation analysis with neuronal spatial distribution data to reveal the unique effects of different types of neurons on muscle functional pathways.In terms of spatial distribution patterns,sympathetic neurons exhibited a more concentrated distribution than sensory and motor neurons.In addition,the neuronal innervation of limb muscles exhibited four different characteristics:sympathetic neuron-rich muscle,sensory neuron-rich muscle,neuron-sparse muscle,and motor neuron-rich muscle.Sensory neuron density was mainly associated with muscle contractile structure and cell pH,whereas sympathetic neuron density was associated with protein kinase activity,muscle vasculature,muscle calcium-dependent protein kinase activity,lipid transport,and vesicle release.Motor neuron density was mainly associated with protein kinase activity,cell adhesion,oxidoreductase activity,and exocytosis.These findings may contribute to a deeper understanding of how nerves cooperate to endow muscles with diverse physiological functions,thereby providing new insights and experimental evidence for the treatment of various neuromuscular diseases.
文摘Exogenous neural stem cell transplantation has become one of the most promising treatment methods for chronic stroke.Recent studies have shown that most ischemia-reperfusion model rats recover spontaneously after injury,which limits the ability to observe long-term behavioral recovery.Here,we used a severe stroke rat model with 150 minutes of ischemia,which produced severe behavioral deficiencies that persisted at 12 weeks,to study the therapeutic effect of neural stem cells on neural restoration in chronic stroke.Our study showed that stroke model rats treated with human neural stem cells had long-term sustained recovery of motor function,reduced infarction volume,long-term human neural stem cell survival,and improved local inflammatory environment and angiogenesis.We also demonstrated that transplanted human neural stem cells differentiated into mature neurons in vivo,formed stable functional synaptic connections with host neurons,and exhibited the electrophysiological properties of functional mature neurons,indicating that they replaced the damaged host neurons.The findings showed that human fetal-derived neural stem cells had long-term effects for neurological recovery in a model of severe stroke,which suggests that human neural stem cells-based therapy may be effective for repairing damaged neural circuits in stroke patients.
基金funded by the National Key Research and Development Program of China(2024YFD1501602)the National Natural Science Foundation of China(42407437)conducted as part of the Coordinated Research Project D1.50.16,implemented by the Soil and Water Management and Crop Nutrition Section of the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture,Department of Nuclear Sciences and Applications,Vienna,Austria。
文摘Nitrogen use efficiency in rice is lower than in upland crops,likely due to differences in soil nitrogen dynamics and crop nitrogen preferences.However,the specific nitrogen dynamics in paddy and upland systems and their impact on crop nitrogen uptake remain poorly understood.The N dynamics and impact on crop N uptake determine the downstream environmental pollution from nitrogen fertilizer.To address this poor understanding,we analyzed 2,044 observations of gross nitrogen transformation rates in soils from 136 studies to examine nitrogen dynamics in both systems and their effects on nitrogen uptake in rice and upland crops.Our findings revealed that nitrogen mineralization and autotrophic nitrification rates are lower in paddies than in upland soil,while dissimilatory nitrate reduction to ammonium is higher in paddies,these differences being driven by flooding and lower total nitrogen content in paddies.Rice exhibited higher ammonium uptake,while upland crops had over twice the nitrate uptake.Autotrophic nitrification stimulated by p H reduced rice nitrogen uptake,while heterotrophic nitrification enhanced nitrogen uptake of upland crops.Autotrophic nitrification played a key role in regulating the ammonium-to-nitrate ratio in soils,which further affected the balance of plant nitrogen uptake.These results highlight the need to align soil nitrogen dynamics with crop nitrogen preferences to maximize plant maximize productivity and reduce reactive nitrogen pollution.
基金financially supported by the Hainan Province Science and Technology Special Fund(Grant no:ZDYF2024XDNY187).
文摘Background:Excessive use of inorganic trace minerals(ITMs)in swine production leads to high fecal mineral excretion and environmental risks,while most studies on organic trace minerals(OTMs)focus on single elements,with limited data on the synergistic effects and molecular mechanisms of combined OTMs(Fe,Cu,Mn,Zn)in growing-finishing pigs.Methods:This study aimed to investigate the effects of graded levels of micromineral proteinates(combined OTMs)on growth performance,mineral metabolism,and mRNA expression of mineral regulatory proteins.A total of 360 crossbred Duroc×Landrace×Large White pigs(initial body weight 47.1±4.8 kg)were randomly assigned to 6 dietary treatments:basal diet without microminerals(CON),basal diet with ITMs at commercially recommended levels(IT),and basal diets with 15%(OT 15%),25%(OT 25%),35%(OT 35%)commercially recommended levels(CRL)of combined micromineral proteinates.After a 70-day feeding trial,samples were analyzed using ICP-OES,ELISA,and RT-qPCR.Results:Results showed that reduced levels(15-35%CRL)of micromineral proteinates did not significantly affect average daily gain,average daily feed intake,or feed conversion ratio(gain-to-feed ratio)compared to IT(P>0.05),but significantly increased plasma Cu(1.73-1.83μg/mL)and Zn(1.72-1.97μg/mL)concentrations(P<0.05)and elevated activities of Cu/Zn-superoxide dismutase(32.9-35.9 U/L)and manganese superoxide dismutase(20.5-24.1 U/L)compared to CON(P<0.05),with no significant differences from IT(P>0.05).Fecal excretion of Fe,Cu,Mn,and Zn was significantly reduced by 35-50%in OT 15%-OT 35%groups compared to IT(P<0.05).OT 25%group exhibited the highest apparent absorptivity of Fe(38.5%),Cu(27.8%),and Zn(42.4%)(P<0.05),which was associated with significantly regulated mRNA expression of mineral regulatory proteins:upregulated DMT1,FPN1,ZIP4,and MT1A in the duodenum,and modulated HAMP,ATP7B,ZIP14,and ZnT1 in the liver(P<0.05).Conclusion:In conclusion,dietary supplementation with 25%CRL or less of combined micromineral proteinates can fully meet the nutritional needs of growing-finishing pigs,improve mineral absorptivity,and reduce fecal mineral excretion by regulating intestinal and hepatic mineral transport and homeostatic proteins,providing a sustainable alternative to high-dose ITMs.
文摘In Brazil, the use of software and applications in railway geometric designs is rare. Currently, after the division and privatization of the national rail network, the concessionaires who assumed the administration, are investing in modernization, recovery and extension of this transport model to make it more efficient. The PIL （Program of Investment in Logistics） has generated growth of the sector in response to the amount of investments, greatly enhancing the load capacity and optimizing the geometric design, which would allow speed of 80 km/h. Based on this information, the objective of this research is to verify the software applications of Power Rail Track Program of Bentley Systems, Inc. for geometric designs in future deployments of railways in Brazil. This software is based on numerical calculations, including roller adjustment by the least squares method to calculate and choose the best alignments, and regression tools, to a fast and effectively optimization of various parameters of the geometry for a future or existing railway. Different variables that allow you to optimize the geometric design are analyzed, considering several optional alignments and complex restrictions present in geometric design, taking into account variables such as circular curves, transition, tangents and rays. The software regression tools include curvature diagrams identifying points and curves, tangent and spirals. The analysis results are presented quickly and points can be added or deleted from the regression analysis, providing improved tracing. The software also allows to detail the elements of the executive project of the railway line such as rails, joints, turning points, waypoints and others. The result of this study has shown that the application of the Power Rail Track Program generates significant cost savings in time and increases in productivity, providing greater opportunity to optimize the geometry and reducing geometric design costs.
文摘Automated Program Repair(APR)techniques have shown significant potential in mitigating the cost and complexity associated with debugging by automatically generating corrective patches for software defects.Despite considerable progress in APR methodologies,existing approaches frequently lack contextual awareness of runtime behaviors and structural intricacies inherent in buggy source code.In this paper,we propose a novel APR approach that integrates attention mechanisms within an autoencoder-based framework,explicitly utilizing structural code affinity and execution context correlation derived from stack trace analysis.Our approach begins with an innovative preprocessing pipeline,where code segments and stack traces are transformed into tokenized representations.Subsequently,the BM25 ranking algorithm is employed to quantitatively measure structural code affinity and execution context correlation,identifying syntactically and semantically analogous buggy code snippets and relevant runtime error contexts from extensive repositories.These extracted features are then encoded via an attention-enhanced autoencoder model,specifically designed to capture significant patterns and correlations essential for effective patch generation.To assess the efficacy and generalizability of our proposed method,we conducted rigorous experimental comparisons against DeepFix,a state-of-the-art APR system,using a substantial dataset comprising 53,478 studentdeveloped C programs.Experimental outcomes indicate that our model achieves a notable bug repair success rate of approximately 62.36%,representing a statistically significant performance improvement of over 6%compared to the baseline.Furthermore,a thorough K-fold cross-validation reinforced the consistency,robustness,and reliability of our method across diverse subsets of the dataset.Our findings present the critical advantage of integrating attentionbased learning with code structural and execution context features in APR tasks,leading to improved accuracy and practical applicability.Future work aims to extend the model’s applicability across different programming languages,systematically optimize hyperparameters,and explore alternative feature representation methods to further enhance debugging efficiency and effectiveness.
基金supported by the National Natural Science Foundation of China(Nos.62104045,52101213)Jiangsu Provincial Department of Science and Technology of China(No.BE2022426).
文摘As the chemical industry expands,the use of benzene,toluene,and xylene(collectively known as BTX)in industrial production has increased greatly.Meanwhile,the toxic nature and potential health hazards of BTX gases cannot be ignored due to low-concentration leaks underline the critical need for rapid and real-time monitoring of these gases.Chemiresistive metal oxide semiconductor(MOS)-based gas sensors,which are extensively used for gas detection in both industrial settings and everyday life,emerge as one of the optimal solutions for trace BTX detection.These sensors are highly valued for their high sensitivity and low detection limits.Nevertheless,the improvement of selectivity towards specific BTX gases to achieve efficient and precise detection still remains challenging.This review summarizes the chemiresistive MOS-based gas sensors designed for BTX detection,categorizing them based on the components of sensing materials-basically into three groups:single-component,single heterojunction,and multiple heterojunctions gas sensing materials.Further,the review proposes the future application prospects of chemiresistive MOS-based BTX gas sensors,with specific emphasis on their significance in promoting industrial safety and environmental monitoring.
基金supported by the National Natural Science Foundation of China (31671119 and 31871090)the Shenzhen Science and Technology Innovation Commission (JCYJ20160428164440255, JCYJ20170413162938668, JCYJ20170818155056369, and JCYJ20170307170742519)+3 种基金the Shenzhen Discipline Construction Project for Neurobiology (DRCSM [2016]1379)the Japan Society for the Promotion of Science KAKENHI (JP18K08494) the Japan Science and Technology Agency PRESTO (JPMJPR1784)the Ono Medical Research Foundation, and the Novartis Foundation (Japan) for the Promotion of Science
文摘A central objective in deciphering the nervous system in health and disease is to define the connections of neurons. The propensity of neurotropic viruses to spread among synaptically-linked neurons makes them ideal for mapping neural circuits. So far, several classes of viral neuronal tracers have become available and provide a powerful toolbox for delineating neural networks. In this paper, we review the recent developments of neurotropic viral tracers and highlight their unique properties in revealing patterns of neuronal connections.
