Understanding the interaction between groundwater and surface water in permafrost regions is essential to study flood frequencies and river water quality, especially in the high latitude/altitude basins. The applicati...Understanding the interaction between groundwater and surface water in permafrost regions is essential to study flood frequencies and river water quality, especially in the high latitude/altitude basins. The application of heat tracing method,based on oscillating streambed temperature signals, is a promising geophysical method for identifying and quantifying the interaction between groundwater and surface water. Analytical analysis based on a one-dimensional convective-conductive heat transport equation combined with the fiber-optic distributed temperature sensing method was applied on a streambed of a mountainous permafrost region in the Yeniugou Basin, located in the upper Heihe River on the northern Tibetan Plateau. The results indicated that low connectivity existed between the stream and groundwater in permafrost regions.The interaction between surface water and groundwater increased with the thawing of the active layer. This study demonstrates that the heat tracing method can be applied to study surface water-groundwater interaction over temporal and spatial scales in permafrost regions.展开更多
The classical Lagrangian particle tracing method is widely used in the evaluation of the ocean annual subduction rate.However,our analysis indicates that in addition to neglecting the effect of mixing,there are two po...The classical Lagrangian particle tracing method is widely used in the evaluation of the ocean annual subduction rate.However,our analysis indicates that in addition to neglecting the effect of mixing,there are two possible deviations in the method:one is an overestimation due to not considering that the amount of subducted water at the source location may be inadequate during the late winter of the first year when the mixed layer becomes shallow;the other one is an underestimation due to the neglect of the effective subduction caused by strong vertical pumping.Quantitative analysis shows that these two deviations mainly exist in the low-latitude subduction areas of the South Pacific and South Atlantic.The two deviations have very similar distribution areas and can partially off set each other.However,the overall deviation is still large,and the maximum relative deviation ratio can reach 50%;therefore,it cannot be ignored.展开更多
The intersection method is one of the basic approaches for locating earthquakes and is not only robust but also efficient. However, its location accuracy is not high, especially for focal depth because the velocity mo...The intersection method is one of the basic approaches for locating earthquakes and is not only robust but also efficient. However, its location accuracy is not high, especially for focal depth because the velocity model used for the conventional intersection method is based on homogeneous or laterally homogeneous media, which is too simple. In order to improve the accuracy, we have modified the existing intersection method. In the modified approach, the earthquake loci are not assumed to be circular or hyperbolic and calculation accuracy is improved using a minimum traveltime tree algorithm for tracing rays. The numerical model shows that the modified method can locate earthquakes in complex velocity models.展开更多
This paper presents a method for tracing a planar implicit curve f（x, y）=0 on a rectangular region based on continuation scheme. First, according to the starting track-point and the starting track-direction of the c...This paper presents a method for tracing a planar implicit curve f（x, y）=0 on a rectangular region based on continuation scheme. First, according to the starting track-point and the starting track-direction of the curve, make a new fimction F（x, y）=0 where the same curve withf（x, y）=0 is defined. Then we trace the curve between the two domains where F（x, y）〉0 and F（x, y）〈0 alternately, according to the two rules presented in this paper. Equal step size or adaptive step size can be used, when we trace the curve. An irregular planar implicit curve （such as the curve with large curvatures at some points on the curve）, can be plotted if an adaptive step size is used. Moreover, this paper presents a scheme to search for the multiple points on the curve. Our method has the following advantages： （1） it can plot Co planar implicit curves; （2） it can plot the planar implicit curves with multiple points; （3） by the help of using the two rules, our method does not need to compute the tangent vector at the points on the curve, and directly searches for the direction of the tracing curve; （4） the tracing procedure costs only one of two evaluations of function f（x, y）=0 per moving step, while most existing similar methods cost more evaluations of the function.展开更多
Objective:To develop a rapid,cost effective RT-PCR method for the mass scale diagnosis of such diseases at the vireraia stage to find out the actual disease burden in that area.Methods:For this purpose,cases with the ...Objective:To develop a rapid,cost effective RT-PCR method for the mass scale diagnosis of such diseases at the vireraia stage to find out the actual disease burden in that area.Methods:For this purpose,cases with the history of only short febrile illness were considered.Thus 157 samples with the history of dengue/chikungunya like illness and only 58 samples with a history of acute encephalitis syndrome(AES)were selected.Results:Out of 157 samples,42 and 74 were detected as dengue and chikungunya,respectively and out of 58 AES cases only 23 could be detected as Japanese encephalitis by this RT-PCR method.Conclusions:This cost effective RT-PCR method can detect the total positive cases that remain undetected by EL1SA method.Moreover,this method is capable to detect the viral RNA from patients'sera even after the appearance of IgM antibody at one fifth costs as compared with the other commercially available kits.展开更多
A new method of NAA for sediment movement study is presented in this paper. Through analyzing the natural sediment, the information about natural tracing agents has been obtained, which can show the direction of sedim...A new method of NAA for sediment movement study is presented in this paper. Through analyzing the natural sediment, the information about natural tracing agents has been obtained, which can show the direction of sediment movement and relative discharge of sediment transportation. Test results in some areas, such as Shantou Harbour and Hangzhou Bay, are in good agreement with those from normal hydrologic measurements and some results are not obtainable with the latter. The NAA is economical, practical and reliable, and should be widely used in engineering practice.展开更多
Generally speaking, the factors of both medium and tectonic that give rise to heterogeneity of the earth crust and mantle structures should be taken into account simultaneously in three dimensional seismic ray tracin...Generally speaking, the factors of both medium and tectonic that give rise to heterogeneity of the earth crust and mantle structures should be taken into account simultaneously in three dimensional seismic ray tracing. In this paper, the three dimensional structure models are constructed with the model similar to generation system in computer aid design and manufacturing (CAD/CAM). Based on the algorithm proposed by Cerveny et al . for complete seismic ray tracing in complex three dimensional structures, a new technique called the indirect approach method for two point seismic ray tracing in three dimensional laterally heterogeneous media has been put forward, and its numerical computing examples were given.展开更多
Although previous investigations of the trace elements in snow and ice from the Qinghai-Tibetan Plateau obtained interesting information about pollution from human activities on the plateau, most were based on traditi...Although previous investigations of the trace elements in snow and ice from the Qinghai-Tibetan Plateau obtained interesting information about pollution from human activities on the plateau, most were based on traditional acidification methods.To emphasize the influence of the different sample-preparation methods on the records of trace elements and rare earth elements, snow samples were collected from glaciers on the Qinghai-Tibetan Plateau in China and prepared using two methods: traditional acidification and total digestion. Concentrations of 18 trace elements(Al, Ti, Fe, Rb, Sr, Ba, V, Cr, Mn, Li,Cu, Co, Mo, Cs, Sb, Pb, Tl, and U), along with 14 rare earth elements(REEs: La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er,Tm, Yb, and Lu), Y, and Th in the snow samples, were measured using inductively coupled plasma-sector field mass spectrometry(ICP-SFMS). The results showed that the mass fraction of the trace elements(defined as ratio of concentration in the acid-leachable fraction to that in the digested sample) such as Mo, Ti, Al, Rb, and V, varied from 0.06 to 0.5. The mass fraction of other trace elements varied from about 0.6 to more than 0.9; those of the REEs, Y, and Th varied from 0.34 to0.75. Lower mass fractions will lead to an overestimated contribution of other sources, especially human activities, and the underestimated fluxes of these trace elements(especially REEs, Y, and Th, as well as dust) if the REEs are used as the proxy for the crust dust. The two sample-preparation methods exhibited different REE normalized distribution patterns,REE ratios, and provenance-tracing results. The REE normalized distribution patterns and proxies in the digested samples are more reliable and integrated than those found in traditional acidification method for dust-provenance tracing.展开更多
The accurate power measurement is important for an ECRH system in tokamak.The dummy load is designed and developed for the measurement of the millimeter wave power.This work analyzes the dummy load based on the quasi-...The accurate power measurement is important for an ECRH system in tokamak.The dummy load is designed and developed for the measurement of the millimeter wave power.This work analyzes the dummy load based on the quasi-optical method and the ray tracing method.The reflectivity and thermal deposition of the dummy load have been considered to ensure the safety of the entire system.High-power tests have been carried out at a 105 GHz/500 kW ECRH system.The results of the tests indicate that the designed dummy load is stable and valid.展开更多
This paper presents a gradient-descent travel time tomography method for solving the acoustictype velocity model inversion problem.Similarly to the adjoint-state method,the proposed method is based on the Eikonal equa...This paper presents a gradient-descent travel time tomography method for solving the acoustictype velocity model inversion problem.Similarly to the adjoint-state method,the proposed method is based on the Eikonal equation,enabling simultaneous calculation of contributions from all common-source receivers to the gradient.This overcomes the inefficiency inherent in conventional travel time tomography methods,which rely on a two-point ray tracing process.By directly calculating Fréchet derivatives,our method avoids the complex derivation processes associated with the adjoint-state method.The key to calculating the Fréchet derivatives is to calculate a so-called ray-path term.Consequently,compared to the adjoint-state method,the proposed method can explicitly obtain the ray paths,resulting in a more concise and intuitive derivation process.Furthermore,our method retains the benefits of the adjoint-state method,such as speed,low memory usage,and robustness.This paper focuses on elucidating the principles and algorithms for calculating the raypath term based on the fast sweeping method.The algorithms could be further speeded up by using parallel computational techniques.Synthetic tests demonstrate that our proposed travel time tomographic method accurately calculates ray paths,regardless of the complexity of the model and recording geometry.展开更多
Submarine groundwater discharge(SGD)is an important part in the land-sea interactions,which mainly contains three components:submarine fresh groundwater discharge(SFGD),tidal flat recirculated saline groundwater disch...Submarine groundwater discharge(SGD)is an important part in the land-sea interactions,which mainly contains three components:submarine fresh groundwater discharge(SFGD),tidal flat recirculated saline groundwater discharge(tidal flat RSGD)and subtidal recirculated saline groundwater discharge(subtidal RSGD).In order to make a more accurate assessment of the impact of SGD on coastal ecological environment,it is necessary to distinguish the main components of SGD.In this study,the Maowei Sea,located in the northern part of the Beibu Gulf,was selected as the study area.Based on the radium(Ra)tracing method,we present a new analytical method for distinguishing the three main components of SGD in this area combined with field data.The average daily flow along the coastline of the Maowei Sea of tidal flat RSGD was slightly higher than that of SFGD,and both two were on the magnitude of 1×10^(5)m^(3)/d.The average daily flow for the subtidal RSGD of the entire subtidal zone of the Maowei Sea reached to the magnitude of 1×10^(6)-1×10^(7)m^(3)/d.The long-term variation trend of terrestrial SGD is a valuable information for the study of the influence of terrigenous material on the coastal ecological environment.Based on the results of four sampling periods,it is found that the fluxes of SFGD and tidal flat RSGD in the Maowei Sea had good linear correlation with the net precipitation.As an example,January 2015 to August 2022 were selected as the study periods,and the variation trends of SFGD and tidal flat RSGD were calculated by linear function with net precipitation as the independent variable.The results showed that the flux of tidal flat RSGD was slightly higher than that of SFGD,and the difference between the two is larger in flood season while smaller in dry season.In general,in the coastal range of China,the total SGD flux in the Maowei Sea area is at a high level,and the SFGD flux is at a medium level.展开更多
With the help of technique of integration within an ordered product of operators we can fully display the partial trace method to deduce some new density operators of light field. The general form of single-mode densi...With the help of technique of integration within an ordered product of operators we can fully display the partial trace method to deduce some new density operators of light field. The general form of single-mode density operator is derived in this way from a normalized two-mode generalized squeezed pure state density operator.展开更多
A型牛轮状病毒(BRV-A)是引起牛腹泻的重要病原之一,对养牛业危害最大。为建立一种针对BRV-A的快速、高通量、低成本检测方法,根据BRV-A的VP6基因保守区设计特异性引物,建立了检测BRV-A的SYBR Green I RT-qPCR方法。该方法对浓度梯度为4....A型牛轮状病毒(BRV-A)是引起牛腹泻的重要病原之一,对养牛业危害最大。为建立一种针对BRV-A的快速、高通量、低成本检测方法,根据BRV-A的VP6基因保守区设计特异性引物,建立了检测BRV-A的SYBR Green I RT-qPCR方法。该方法对浓度梯度为4.41×10^(8)~4.41×10^(2) copies/μL质粒标准品的扩增Ct值与拷贝数浓度呈良好的线性关系,熔解曲线为单峰;与其他引起牛腹泻的常见病原无交叉反应,最低检测限为4.41×10^(1) copies/μL,批内、批间重复性试验Ct值变异系数均低于1%;对临床粪便样品的阳性检出率高于地方标准中的PCR方法,且检测结果符合性较好。综上,本研究建立的BRV-A SYBR Green I RT-qPCR检测方法灵敏、特异、稳定,且操作简单、成本低,为临床样品的大规模BRV-A检测及其感染的早期诊断提供了技术支撑。展开更多
基金supported by the National Natural Science Foundation of China(41690141,41671067)the second Tibetan Plateau Scientific Expedition and Research Program(STEP)(2019QZKK0605)+1 种基金the Fundamental Research Funds for Central Universities(lzujbky-2019-40)CAS"Light of West China"and the State Key Laboratory of Cryospheric Science,CAS(SKLCS-ZZ-2020).
文摘Understanding the interaction between groundwater and surface water in permafrost regions is essential to study flood frequencies and river water quality, especially in the high latitude/altitude basins. The application of heat tracing method,based on oscillating streambed temperature signals, is a promising geophysical method for identifying and quantifying the interaction between groundwater and surface water. Analytical analysis based on a one-dimensional convective-conductive heat transport equation combined with the fiber-optic distributed temperature sensing method was applied on a streambed of a mountainous permafrost region in the Yeniugou Basin, located in the upper Heihe River on the northern Tibetan Plateau. The results indicated that low connectivity existed between the stream and groundwater in permafrost regions.The interaction between surface water and groundwater increased with the thawing of the active layer. This study demonstrates that the heat tracing method can be applied to study surface water-groundwater interaction over temporal and spatial scales in permafrost regions.
基金Supported by the National Natural Science Foundation of China(No.41676009)the National Key R&D Program of China(No.2016YFC0301203)the State Key Program of National Natural Science of China(No.41730534)。
文摘The classical Lagrangian particle tracing method is widely used in the evaluation of the ocean annual subduction rate.However,our analysis indicates that in addition to neglecting the effect of mixing,there are two possible deviations in the method:one is an overestimation due to not considering that the amount of subducted water at the source location may be inadequate during the late winter of the first year when the mixed layer becomes shallow;the other one is an underestimation due to the neglect of the effective subduction caused by strong vertical pumping.Quantitative analysis shows that these two deviations mainly exist in the low-latitude subduction areas of the South Pacific and South Atlantic.The two deviations have very similar distribution areas and can partially off set each other.However,the overall deviation is still large,and the maximum relative deviation ratio can reach 50%;therefore,it cannot be ignored.
基金This work is supported by the National Natural Science Foundation of China(40674044)the Special Foundation for Basic Professional Scientific Research (DQJB06A02)
文摘The intersection method is one of the basic approaches for locating earthquakes and is not only robust but also efficient. However, its location accuracy is not high, especially for focal depth because the velocity model used for the conventional intersection method is based on homogeneous or laterally homogeneous media, which is too simple. In order to improve the accuracy, we have modified the existing intersection method. In the modified approach, the earthquake loci are not assumed to be circular or hyperbolic and calculation accuracy is improved using a minimum traveltime tree algorithm for tracing rays. The numerical model shows that the modified method can locate earthquakes in complex velocity models.
文摘This paper presents a method for tracing a planar implicit curve f（x, y）=0 on a rectangular region based on continuation scheme. First, according to the starting track-point and the starting track-direction of the curve, make a new fimction F（x, y）=0 where the same curve withf（x, y）=0 is defined. Then we trace the curve between the two domains where F（x, y）〉0 and F（x, y）〈0 alternately, according to the two rules presented in this paper. Equal step size or adaptive step size can be used, when we trace the curve. An irregular planar implicit curve （such as the curve with large curvatures at some points on the curve）, can be plotted if an adaptive step size is used. Moreover, this paper presents a scheme to search for the multiple points on the curve. Our method has the following advantages： （1） it can plot Co planar implicit curves; （2） it can plot the planar implicit curves with multiple points; （3） by the help of using the two rules, our method does not need to compute the tangent vector at the points on the curve, and directly searches for the direction of the tracing curve; （4） the tracing procedure costs only one of two evaluations of function f（x, y）=0 per moving step, while most existing similar methods cost more evaluations of the function.
基金supported by the Department of Science and Technology,Goverment of West Bengal.India[grant No.705(Sanc.)ST/P/S&T/9G-27/2007]
文摘Objective:To develop a rapid,cost effective RT-PCR method for the mass scale diagnosis of such diseases at the vireraia stage to find out the actual disease burden in that area.Methods:For this purpose,cases with the history of only short febrile illness were considered.Thus 157 samples with the history of dengue/chikungunya like illness and only 58 samples with a history of acute encephalitis syndrome(AES)were selected.Results:Out of 157 samples,42 and 74 were detected as dengue and chikungunya,respectively and out of 58 AES cases only 23 could be detected as Japanese encephalitis by this RT-PCR method.Conclusions:This cost effective RT-PCR method can detect the total positive cases that remain undetected by EL1SA method.Moreover,this method is capable to detect the viral RNA from patients'sera even after the appearance of IgM antibody at one fifth costs as compared with the other commercially available kits.
文摘A new method of NAA for sediment movement study is presented in this paper. Through analyzing the natural sediment, the information about natural tracing agents has been obtained, which can show the direction of sediment movement and relative discharge of sediment transportation. Test results in some areas, such as Shantou Harbour and Hangzhou Bay, are in good agreement with those from normal hydrologic measurements and some results are not obtainable with the latter. The NAA is economical, practical and reliable, and should be widely used in engineering practice.
文摘Generally speaking, the factors of both medium and tectonic that give rise to heterogeneity of the earth crust and mantle structures should be taken into account simultaneously in three dimensional seismic ray tracing. In this paper, the three dimensional structure models are constructed with the model similar to generation system in computer aid design and manufacturing (CAD/CAM). Based on the algorithm proposed by Cerveny et al . for complete seismic ray tracing in complex three dimensional structures, a new technique called the indirect approach method for two point seismic ray tracing in three dimensional laterally heterogeneous media has been put forward, and its numerical computing examples were given.
基金supported by grants provided by the National Natural Science Foundation of China(Grant Nos.41276194,40771046,and 40601021)
文摘Although previous investigations of the trace elements in snow and ice from the Qinghai-Tibetan Plateau obtained interesting information about pollution from human activities on the plateau, most were based on traditional acidification methods.To emphasize the influence of the different sample-preparation methods on the records of trace elements and rare earth elements, snow samples were collected from glaciers on the Qinghai-Tibetan Plateau in China and prepared using two methods: traditional acidification and total digestion. Concentrations of 18 trace elements(Al, Ti, Fe, Rb, Sr, Ba, V, Cr, Mn, Li,Cu, Co, Mo, Cs, Sb, Pb, Tl, and U), along with 14 rare earth elements(REEs: La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er,Tm, Yb, and Lu), Y, and Th in the snow samples, were measured using inductively coupled plasma-sector field mass spectrometry(ICP-SFMS). The results showed that the mass fraction of the trace elements(defined as ratio of concentration in the acid-leachable fraction to that in the digested sample) such as Mo, Ti, Al, Rb, and V, varied from 0.06 to 0.5. The mass fraction of other trace elements varied from about 0.6 to more than 0.9; those of the REEs, Y, and Th varied from 0.34 to0.75. Lower mass fractions will lead to an overestimated contribution of other sources, especially human activities, and the underestimated fluxes of these trace elements(especially REEs, Y, and Th, as well as dust) if the REEs are used as the proxy for the crust dust. The two sample-preparation methods exhibited different REE normalized distribution patterns,REE ratios, and provenance-tracing results. The REE normalized distribution patterns and proxies in the digested samples are more reliable and integrated than those found in traditional acidification method for dust-provenance tracing.
基金supported by the National Key Research and Development Program of China (Nos. 2017YFE0300200 and 2017YFE0300204)part by National Natural Science Foundation of China (No. 51821005)
文摘The accurate power measurement is important for an ECRH system in tokamak.The dummy load is designed and developed for the measurement of the millimeter wave power.This work analyzes the dummy load based on the quasi-optical method and the ray tracing method.The reflectivity and thermal deposition of the dummy load have been considered to ensure the safety of the entire system.High-power tests have been carried out at a 105 GHz/500 kW ECRH system.The results of the tests indicate that the designed dummy load is stable and valid.
基金supported by 14th Five-Year Plan major science and technology projects(no.KJGG2022-0201)。
文摘This paper presents a gradient-descent travel time tomography method for solving the acoustictype velocity model inversion problem.Similarly to the adjoint-state method,the proposed method is based on the Eikonal equation,enabling simultaneous calculation of contributions from all common-source receivers to the gradient.This overcomes the inefficiency inherent in conventional travel time tomography methods,which rely on a two-point ray tracing process.By directly calculating Fréchet derivatives,our method avoids the complex derivation processes associated with the adjoint-state method.The key to calculating the Fréchet derivatives is to calculate a so-called ray-path term.Consequently,compared to the adjoint-state method,the proposed method can explicitly obtain the ray paths,resulting in a more concise and intuitive derivation process.Furthermore,our method retains the benefits of the adjoint-state method,such as speed,low memory usage,and robustness.This paper focuses on elucidating the principles and algorithms for calculating the raypath term based on the fast sweeping method.The algorithms could be further speeded up by using parallel computational techniques.Synthetic tests demonstrate that our proposed travel time tomographic method accurately calculates ray paths,regardless of the complexity of the model and recording geometry.
基金The National Natural Science Foundation of China under contract Nos 41576083 and 41906150the National Key R&D Program of China under contract No.2022YFE0209300.
文摘Submarine groundwater discharge(SGD)is an important part in the land-sea interactions,which mainly contains three components:submarine fresh groundwater discharge(SFGD),tidal flat recirculated saline groundwater discharge(tidal flat RSGD)and subtidal recirculated saline groundwater discharge(subtidal RSGD).In order to make a more accurate assessment of the impact of SGD on coastal ecological environment,it is necessary to distinguish the main components of SGD.In this study,the Maowei Sea,located in the northern part of the Beibu Gulf,was selected as the study area.Based on the radium(Ra)tracing method,we present a new analytical method for distinguishing the three main components of SGD in this area combined with field data.The average daily flow along the coastline of the Maowei Sea of tidal flat RSGD was slightly higher than that of SFGD,and both two were on the magnitude of 1×10^(5)m^(3)/d.The average daily flow for the subtidal RSGD of the entire subtidal zone of the Maowei Sea reached to the magnitude of 1×10^(6)-1×10^(7)m^(3)/d.The long-term variation trend of terrestrial SGD is a valuable information for the study of the influence of terrigenous material on the coastal ecological environment.Based on the results of four sampling periods,it is found that the fluxes of SFGD and tidal flat RSGD in the Maowei Sea had good linear correlation with the net precipitation.As an example,January 2015 to August 2022 were selected as the study periods,and the variation trends of SFGD and tidal flat RSGD were calculated by linear function with net precipitation as the independent variable.The results showed that the flux of tidal flat RSGD was slightly higher than that of SFGD,and the difference between the two is larger in flood season while smaller in dry season.In general,in the coastal range of China,the total SGD flux in the Maowei Sea area is at a high level,and the SFGD flux is at a medium level.
基金Supported by the President Foundation of Chinese Academy of Science and National Natural Science Foundation of China under Grant Nos.10775097 and 10874174
文摘With the help of technique of integration within an ordered product of operators we can fully display the partial trace method to deduce some new density operators of light field. The general form of single-mode density operator is derived in this way from a normalized two-mode generalized squeezed pure state density operator.
文摘A型牛轮状病毒(BRV-A)是引起牛腹泻的重要病原之一,对养牛业危害最大。为建立一种针对BRV-A的快速、高通量、低成本检测方法,根据BRV-A的VP6基因保守区设计特异性引物,建立了检测BRV-A的SYBR Green I RT-qPCR方法。该方法对浓度梯度为4.41×10^(8)~4.41×10^(2) copies/μL质粒标准品的扩增Ct值与拷贝数浓度呈良好的线性关系,熔解曲线为单峰;与其他引起牛腹泻的常见病原无交叉反应,最低检测限为4.41×10^(1) copies/μL,批内、批间重复性试验Ct值变异系数均低于1%;对临床粪便样品的阳性检出率高于地方标准中的PCR方法,且检测结果符合性较好。综上,本研究建立的BRV-A SYBR Green I RT-qPCR检测方法灵敏、特异、稳定,且操作简单、成本低,为临床样品的大规模BRV-A检测及其感染的早期诊断提供了技术支撑。
