Alginate oligosaccharides(AOS)and fucoidan are two important bioactive carbohydrate which that can be prepared from brown seaweeds.We investigated a novel enzyme-assisted one-pot method on AOS and fucoidan extraction ...Alginate oligosaccharides(AOS)and fucoidan are two important bioactive carbohydrate which that can be prepared from brown seaweeds.We investigated a novel enzyme-assisted one-pot method on AOS and fucoidan extraction from Lessonia trabeculata,and found that the optimum extraction procedure was:first,using 10-g/L Na_(2)CO_(3) solution to digest the seaweeds for 2 h at 80℃ with the material-liquid ratio 1:20(g/mL),and then adding 4.0%fucoidan cleavage enzyme for reaction for 12 h at 45℃.The yield of AOS and fucoidan was 21.36%and 14.40%,respectively,which was higher than those using traditional methods.The degree of polymerization of AOS was determined to be 3-5,confirming the results through thin layer chromatography(TLC)and electrospray ionization mass spectrometry(ESI-MS).The ratio of guluronic acid to mannuronic acid in AOS was found to be 1.97.Through anion-exchange column chromatography,four sulfated polysaccharide fractions(F0.5,F1.0,F1.5,and F2.0)were successfully isolated from fucoidan.Chemical analysis revealed that fucoidan and F0.5 were heteropolysaccharides,consisting mainly of mannuronic acid,fucose,and xylose,while the other fractions were predominantly composed of fucose and galactose.The sulfate group content increased from 8.74% to 18.76%,while the uronic acid content decreased from 23.79% to 5.58%across the fractions.All fractions of fucoidan exhibited significant antioxidant activity in various tests.There was a positive correlation between the sulfate content and the ability to scavenge superoxide radicals.Additionally,both AOS and the fucoidan fractions showed immunostimulation activity and were nontoxic to RAW264.7 cells.These results indicate that the novel enzyme-assisted one-pot method is a green and efficient approach for the extraction of fucoidan and alginate oligosaccharides from brown seaweeds.It has potential applications in the processing industry of brown seaweeds.展开更多
In this study, we evaluated the chemical property and antioxidant activity of fucoidans isolated from brown algae, Laminaria japonica(LJF), Lessonia nigrescens(LNF), Lessonia trabeculata(LTF), Ascophyllum mackaii(AMF)...In this study, we evaluated the chemical property and antioxidant activity of fucoidans isolated from brown algae, Laminaria japonica(LJF), Lessonia nigrescens(LNF), Lessonia trabeculata(LTF), Ascophyllum mackaii(AMF), and Ecklonia maxima(EMF). LJF was less in sulfate content(14.16%) and more in galactose and mannose content(1.08 and 0.68) than the documented early. EMF contained 20%–30% of sulfate and fucose, 0.97 in molar ratio which was lower than that of sulfate to other four fucoidans(1.21–1.41). AMF(162 kDa) and EMF(150 kDa) were the first two largest in molecular weight, which were followed by LJP(126 kDa), LNF(113 kDa) and LTF(105 kDa). The fucoidans isolated these algae showed a wide range of antioxidant activity in vitro. It was found that the reducing power of the isolated fucoidans was positively correlated with their sulfate content and molecular weight. In addition, LNF and LTF at low concentrations exhibited high superoxide and hydroxyl radical scavenging activity. This demonstrated that low molecular weight fucoidans may perform a high antioxidant activity.展开更多
基金Supported by the Jiangsu Coast Development Group Co.,Ltd.the Marine Biological High Value Development and Utilization Project(No.2023YHTZZZ04)+1 种基金the Natural Science Foundation of Shandong Province(No.ZR2022MD007)the Science and Technology Project of Fujian Province(Nos.2022T3024,2023T3057)。
文摘Alginate oligosaccharides(AOS)and fucoidan are two important bioactive carbohydrate which that can be prepared from brown seaweeds.We investigated a novel enzyme-assisted one-pot method on AOS and fucoidan extraction from Lessonia trabeculata,and found that the optimum extraction procedure was:first,using 10-g/L Na_(2)CO_(3) solution to digest the seaweeds for 2 h at 80℃ with the material-liquid ratio 1:20(g/mL),and then adding 4.0%fucoidan cleavage enzyme for reaction for 12 h at 45℃.The yield of AOS and fucoidan was 21.36%and 14.40%,respectively,which was higher than those using traditional methods.The degree of polymerization of AOS was determined to be 3-5,confirming the results through thin layer chromatography(TLC)and electrospray ionization mass spectrometry(ESI-MS).The ratio of guluronic acid to mannuronic acid in AOS was found to be 1.97.Through anion-exchange column chromatography,four sulfated polysaccharide fractions(F0.5,F1.0,F1.5,and F2.0)were successfully isolated from fucoidan.Chemical analysis revealed that fucoidan and F0.5 were heteropolysaccharides,consisting mainly of mannuronic acid,fucose,and xylose,while the other fractions were predominantly composed of fucose and galactose.The sulfate group content increased from 8.74% to 18.76%,while the uronic acid content decreased from 23.79% to 5.58%across the fractions.All fractions of fucoidan exhibited significant antioxidant activity in various tests.There was a positive correlation between the sulfate content and the ability to scavenge superoxide radicals.Additionally,both AOS and the fucoidan fractions showed immunostimulation activity and were nontoxic to RAW264.7 cells.These results indicate that the novel enzyme-assisted one-pot method is a green and efficient approach for the extraction of fucoidan and alginate oligosaccharides from brown seaweeds.It has potential applications in the processing industry of brown seaweeds.
文摘将F藻（Lessonia trabeculata）的茎部浸泡后粉碎至1-3 mm进行消化反应,考察了Na2CO3浓度、料液质量比、搅拌转速、消化温度和消化时间对褐藻胶消化得率和褐藻胶溶液粘度的影响,通过正交实验对消化工艺条件进行优化,并对褐藻胶进行红外光谱定性分析.结果表明,最佳消化条件为：温度70℃,Na2CO3浓度1.67%,搅拌转速400 r/min,料液质量比1：15,时间8 h.该条件下褐藻酸钠产品得率为34.80%,粘度为2261.67 m Pa·s,远高于市售产品的173.30m Pa·s.褐藻酸钠基本理化指标均符合国家标准、粘均分子量为11.85 k Da.
基金supported by the Ocean Public Welfare Scientific Research Project, State Ocean Administration of China (No. 201105029-9)
文摘In this study, we evaluated the chemical property and antioxidant activity of fucoidans isolated from brown algae, Laminaria japonica(LJF), Lessonia nigrescens(LNF), Lessonia trabeculata(LTF), Ascophyllum mackaii(AMF), and Ecklonia maxima(EMF). LJF was less in sulfate content(14.16%) and more in galactose and mannose content(1.08 and 0.68) than the documented early. EMF contained 20%–30% of sulfate and fucose, 0.97 in molar ratio which was lower than that of sulfate to other four fucoidans(1.21–1.41). AMF(162 kDa) and EMF(150 kDa) were the first two largest in molecular weight, which were followed by LJP(126 kDa), LNF(113 kDa) and LTF(105 kDa). The fucoidans isolated these algae showed a wide range of antioxidant activity in vitro. It was found that the reducing power of the isolated fucoidans was positively correlated with their sulfate content and molecular weight. In addition, LNF and LTF at low concentrations exhibited high superoxide and hydroxyl radical scavenging activity. This demonstrated that low molecular weight fucoidans may perform a high antioxidant activity.
