The carotenoid-producing strain Rhodosporidium toruloides NP11 and its mutant strain A1-15 were cultivated under salt stress using chemostat cultivation.A multi-omics approach revealed distinct mechanisms between NP11...The carotenoid-producing strain Rhodosporidium toruloides NP11 and its mutant strain A1-15 were cultivated under salt stress using chemostat cultivation.A multi-omics approach revealed distinct mechanisms between NP11 and A1-15 under stress conditions.Salt stress significantly reduced carotenoid production in NP11 but increased it in A1-15,with torularhodin being the predominant carotenoid in A1-15.NP11 exhibited enhanced lipid synthesis under salt stress,suggesting this as its primary adaptive response.In A1-15,reactive oxygen species(ROS)pressure upregulated the CRTI and CRTY genes,with a more pronounced upregulation of CRTI,likely contributing to increased torularhodin production.Additionally,upregulation of the COX7A gene in both strains may support energy demands and mitigate oxidative stress.Notably,this study is the first to mention and explore COX7A in research related to R.toruloides.展开更多
Four non-conventional yeast strains belonging to the genera Yarrowia and Rhodosporidium were cultivated under nitrogen-limited conditions in shake flasks with biodiesel-derived glycerol employed as sole substrate.The ...Four non-conventional yeast strains belonging to the genera Yarrowia and Rhodosporidium were cultivated under nitrogen-limited conditions in shake flasks with biodiesel-derived glycerol employed as sole substrate.The strains R.toruloides DSM 4444 and Y.lipolytica ACA-DC 5033 displayed better performances and were further studied.At initial glycerol(Gly0)≈50 g/L,the strain ACA-DC 5033 produced dry cell weight(DCW)≈7.0 g/L,and also secondary extra-cellular metabolites as citric acid(CA)at≈16 g/L and polyols(Pol)(mannitol,erythritol and arabitol)at≈21 g/L.In double-media(besides nitrogen also Mg was limiting)for Gly0≈50 g/L,a shift towards CA production occurred(CAmax≈33 g/L).The strain DSM 4444 at Gly0≈50 g/L produced DCW=18.1 g/L containing lipids=30.3%in DCW.In single nitrogen-limited media with Gly0≈90 g/L,the strain ACA-DC 5033 produced Pol≈48 g/L and CA≈20 g/L,while the strain DSM 4444 produced DCW=27.3 g/L containing lipid=54.5%w/w.At the late growth phases,metabolites were re-consumed.Balanced growth phase(trophophase)and phase of secondary metabolite synthesis(idiophase)were successfully simulated with the aid of a modified Velhlust-Aggelis model.Lipid extraction process was studied for these two strains by using two different extraction methods.Yeast lipids contained mostly oleic acid,constituting suitable precursors for the synthesis of 2nd generation biodiesel.展开更多
基金supported by the National Natural Science Foundation of China(31972204).
文摘The carotenoid-producing strain Rhodosporidium toruloides NP11 and its mutant strain A1-15 were cultivated under salt stress using chemostat cultivation.A multi-omics approach revealed distinct mechanisms between NP11 and A1-15 under stress conditions.Salt stress significantly reduced carotenoid production in NP11 but increased it in A1-15,with torularhodin being the predominant carotenoid in A1-15.NP11 exhibited enhanced lipid synthesis under salt stress,suggesting this as its primary adaptive response.In A1-15,reactive oxygen species(ROS)pressure upregulated the CRTI and CRTY genes,with a more pronounced upregulation of CRTI,likely contributing to increased torularhodin production.Additionally,upregulation of the COX7A gene in both strains may support energy demands and mitigate oxidative stress.Notably,this study is the first to mention and explore COX7A in research related to R.toruloides.
基金financially supported by the project entitled“Adding value to biodiesel-derived crude glycerol with the use of Chemical and Microbial Technology”(Acronym:Addvalue2glycerol,project codeΤ1ЕΔΚ-03002)financed by the Ministry of National Education and Religious Affairs,Greece(project action:“Investigate-Create-Innovate 2014-2020,Intervention Ⅱ”).
文摘Four non-conventional yeast strains belonging to the genera Yarrowia and Rhodosporidium were cultivated under nitrogen-limited conditions in shake flasks with biodiesel-derived glycerol employed as sole substrate.The strains R.toruloides DSM 4444 and Y.lipolytica ACA-DC 5033 displayed better performances and were further studied.At initial glycerol(Gly0)≈50 g/L,the strain ACA-DC 5033 produced dry cell weight(DCW)≈7.0 g/L,and also secondary extra-cellular metabolites as citric acid(CA)at≈16 g/L and polyols(Pol)(mannitol,erythritol and arabitol)at≈21 g/L.In double-media(besides nitrogen also Mg was limiting)for Gly0≈50 g/L,a shift towards CA production occurred(CAmax≈33 g/L).The strain DSM 4444 at Gly0≈50 g/L produced DCW=18.1 g/L containing lipids=30.3%in DCW.In single nitrogen-limited media with Gly0≈90 g/L,the strain ACA-DC 5033 produced Pol≈48 g/L and CA≈20 g/L,while the strain DSM 4444 produced DCW=27.3 g/L containing lipid=54.5%w/w.At the late growth phases,metabolites were re-consumed.Balanced growth phase(trophophase)and phase of secondary metabolite synthesis(idiophase)were successfully simulated with the aid of a modified Velhlust-Aggelis model.Lipid extraction process was studied for these two strains by using two different extraction methods.Yeast lipids contained mostly oleic acid,constituting suitable precursors for the synthesis of 2nd generation biodiesel.
