Background Sperm cryopreservation is widely used in the cattle industry,as it allows for disassociating the localiza-tion of sires and the collection of semen from the timing of artificial insemination.While freeze-th...Background Sperm cryopreservation is widely used in the cattle industry,as it allows for disassociating the localiza-tion of sires and the collection of semen from the timing of artificial insemination.While freeze-thawing is known to impair sperm DNA integrity,whether the damage induced consists of single-(SSB)or double-strand breaks(DSB)has not been determined.In addition,no previous study has addressed if DNA breaks preferentially reside in specific genome regions such as those forming the toroid linker regions,or are rather spread throughout the regions linked to protamines.The main aim of the present work,therefore,was to elucidate the type and localization of the DNA damage generated by cryopreservation and to evaluate its impact on artificial insemination outcomes in cattle.Results The incidence of SSB and DSB was evaluated in 12 ejaculates before and after cryopreservation with the Comet assay,and the localization of the DNA breaks was assessed using pulsed-field gel electrophoresis(PFGE).Before cryopreservation,the incidence of SSB was 10.99%±4.62%and involved 20.56%±3.04%of sperm cells,whereas these figures significantly(P<0.0001)increased up to 34.11%±3.48%and 53.36%±11.00%in frozen-thawed sperm.In contrast,no significant differences in the incidence of DSB were observed(P>0.990)before and after cryopreservation(before:incidence of 13.91%±1.75%of sperm DNA affecting 56.04%±12.49%of sperm cells;after:incidence of 13.55%±1.55%of sperm DNA involving 53.36%±11.00%of sperm cells).Moreover,PFGE revealed that the percentage of sperm DNA fragments whose length was shorter than a toroid(<31.5 kb)was greater(P<0.0001)after(27.00%±4.26%)than before freeze-thawing(15.57%±4.53%).These differences indicated that the DNA breaks induced by cryopreservation affect the regions condensed in protamines,which are structured in toroids.On the other hand,in vivo fertility rates were associated to the incidence of SSB and DSB in frozen-thawed sperm(P=0.032 and P=0.005),but not with the size of the DNA fragments resulting from these breaks(P>0.05).Conclusion Cryopreservation of bovine sperm generates single-strand DNA breaks,which are mainly located in protamine-condensed toroidal regions.The incidence of DNA breaks in cryopreserved sperm has an impact on cat-tle fertility,regardless of the size of generated fragments.展开更多
Toroidal torques,generated by the resonant magnetic perturbation(RMP)and acting on the plasma column,are numerically systematically investigated for an ITER baseline scenario.The neoclassical toroidal viscosity(NTV),i...Toroidal torques,generated by the resonant magnetic perturbation(RMP)and acting on the plasma column,are numerically systematically investigated for an ITER baseline scenario.The neoclassical toroidal viscosity(NTV),in particular the resonant portion,is found to provide the dominant contribution to the total toroidal torque under the slow plasma flow regime in ITER.While the electromagnetic torque always opposes the plasma flow,the toroidal torque associated with the Reynolds stress enhances the plasma flow independent of the flow direction.A peculiar double-peak structure for the net NTV torque is robustly computed for ITER,as the toroidal rotation frequency is scanned near the zero value.This structure is found to be ultimately due to a non-monotonic behavior of the wave-particle resonance integral(over the particle pitch angle)in the superbanana plateau NTV regime in ITER.These findings are qualitatively insensitive to variations of a range of factors including the wall resistivity,the plasma pedestal flow and the assumed frequency of the rotating RMP field.展开更多
在[4]和[5]中已经研究了sim p ly-laced型T oro idal李代数的顶点表示,[6]文据此给出了Bl型T oro idal李代数顶点表示的构造.受[6]文启发,本文给出了G2型T oro idal李代数的顶点表示的构造,这种构造方式与D(41)的D ynk in图的顶点粘合...在[4]和[5]中已经研究了sim p ly-laced型T oro idal李代数的顶点表示,[6]文据此给出了Bl型T oro idal李代数顶点表示的构造.受[6]文启发,本文给出了G2型T oro idal李代数的顶点表示的构造,这种构造方式与D(41)的D ynk in图的顶点粘合和一个2上循环有着紧密联系.展开更多
We will give an overview of results obtained by our reactive fluid model. It is characterised as a fluid model where all moments with sources in the experiment are kept. Furthermore, full account is taken for the high...We will give an overview of results obtained by our reactive fluid model. It is characterised as a fluid model where all moments with sources in the experiment are kept. Furthermore, full account is taken for the highest moments appearing in unexpanded denominators also including full toroidicity. It has been demonstrated that the strength of zonal flows is dramatically larger in reactive fluid closures than in those which involve dissipation. This gives a direct connection between the fluid closure and the level of excitation of turbulence. This is because zonal flows are needed to absorb the inverse cascade in quasi 2D turbulence. This also explains the similarity in structure of the transport coefficients in our model with a reactive closure in the energy equation and models which have a reactive closure because of zero ion temperature such as the Hasegawa-Wakatani model. Our exact reactive closure unifies several well-known features of tokamak experiments such as the L-H transition, internal transport barriers and the nonlinear Dimits upshift of the critical gradient for onset of transport. It also gives transport of the same level as that in nonlinear gyrokinetic codes. Since these include the kinetic resonance this confirms the validity of the thermodynamic properties of our model. Furthermore, we can show that while a strongly nonlinear model is needed in kinetic theory a quasilinear model is sufficient in the fluid description. Thus our quasilinear fluid model will be adequate for treating all relevant problems in bulk transport. This is finally confirmed by the reproduction by the model of the experimental power scaling of the confinement time Te ~ P-2/3. This confirms the validity of our reactive fluid model. This also gives credibility to our ITER simulations including the H-mode barrier. A new result is here, that alpha heating strongly reduces the slope of the H-mode barrier. This should significantly reduce the effects of ELM＇s.展开更多
By employing dynamic Monte Carlo simulations, we investigate a coil-to-toroid transition of self-attractive semiflexible polymers and the spatial distributions of nanoparticles in self- attractive semiflexible polymer...By employing dynamic Monte Carlo simulations, we investigate a coil-to-toroid transition of self-attractive semiflexible polymers and the spatial distributions of nanoparticles in self- attractive semiflexible polymer/nanoparticle composites. The conformation of self-attractive semiflexible polymers depends on bending energy and self-attractive interactions between monomers in polymer chains. A three-stage process of toroid formation for self-attractive semiflexible chains is shown： several isolated toroids, a loose toroid structure, and a compact toroid structure. Utilizing the compact toroid conformations of self-attractive semiflexible chains, we can control effectively the spatial distributions of nanoparticles in self-attractive semiflexible polymer nanocomposites, and an unconventional toroid structure of nanoparti- cles is observed.展开更多
This paper aims to study the oscillation of a sparkgenerated submerged bubble located near or inside a circular aperture made in a flat plate using high-speed visualization technique. In the case of a bubble oscillati...This paper aims to study the oscillation of a sparkgenerated submerged bubble located near or inside a circular aperture made in a flat plate using high-speed visualization technique. In the case of a bubble oscillating near an aperture the initial free surface of the water was set at the bottom surface of the plate. The effects of aperture size and bubblefree surface distance on the bubble behavior as well as on the ensuing droplet dynamics are investigated. It was found that the direction of the bubble reentrant jet was towards the aperture or away from it respectively when the normalized aperture size was smaller or greater than a certain critical value. In addition, a toroidal vortex ring was observed to form, which rotated inwards as it moved away from the aperture. It was also found that if the bubble was incepted at a distance sufficiently away from a supercritical size aperture a single droplet could be produced. In the case of a bub- ble initiated in the middle of a circular aperture submerged just beneath the water free surface, the bubble was found to take the shape of an ellipsoid during its expansion. Then a reentrant jet was initiated and pierced the bubble from its top side.展开更多
On the basis of the principle of dieless hydrobulging technology, a novel hydrobulging technology for manufacturinghyperbolic plates is proposed. First, a toroidal pipe elbow or a partial toroidal pipe elbow is formed...On the basis of the principle of dieless hydrobulging technology, a novel hydrobulging technology for manufacturinghyperbolic plates is proposed. First, a toroidal pipe elbow or a partial toroidal pipe elbow is formed, then the single hydrobulgedstructure can be cut up into some desired plates. It is proved by experiments and finite element simulation that manufacturingtechnology of hyperbolic plates adopting integrated hydrobulging forming technology is feasible.展开更多
A level set method of non-uniform grids is used to simulate the whole evolution of a cavitation bubble, including its growth, collapse and rebound near a rigid wall. Single-phase Navier-Stokes equation in the liquid r...A level set method of non-uniform grids is used to simulate the whole evolution of a cavitation bubble, including its growth, collapse and rebound near a rigid wall. Single-phase Navier-Stokes equation in the liquid region is solved by MAC projection algorithm combined with second-order ENO scheme for the advection terms. The moving inter-face is captured by the level set function, and the interface velocity is resolved by ＂one-side＂ velocity extension from the liquid region to the bubble region, complementing the second-order weighted least squares method across the interface and projection inside bubble. The use of non-uniform grid overcomes the difficulty caused by the large computational domain and very small bubble size. The computation is very stable without suffering from large flow-field gradients, and the results are in good agreements with other studies. The bubble interface kinematics, dynamics and its effect on the wall are highlighted, which shows that the code can effectively capture the ＂shock wave＂-like pressure and velocity at jet impact, toroidal bubble, and complicated pressure structure with peak, plateau and valley in the later stage of bubble oscillating.展开更多
The most general contact in mechanical transmission is the elliptical one. In particular, a toroidal continuously variable transmission (CVT) has an elliptical shape in the contact area under the elastohydrodynamic lu...The most general contact in mechanical transmission is the elliptical one. In particular, a toroidal continuously variable transmission (CVT) has an elliptical shape in the contact area under the elastohydrodynamic lubrication regime, where the shearing of the fluid subjects to high contact stress and transmits the power. Many parameters affect the contact service performance of the half toroidal CVT, which include the properties of the contacting material (Young抯 modulus), operating parameters (input torque and maximum traction coefficient) and geometrical parameters (aspect cavity ratio, curvature ratio and half cone angle of the power roller). In this paper, the contacts between the input disk, the power roller and the output disk are formulated using the classical Hertzian contact theory. Based on the formulated equations, different system parameters, which affect the maximum Hertzian stresses, are compared. The comparative results will provide some observations of the relations between the maximum Hertzian stresses and transmission ratios in the form of graphs. These graphs give useful information for designer to know the maximum Hertzian stress during operation in such systems.展开更多
基金Ministry of Science,Innovation and Universities,Spain(NextGeneration EU fundsMaría Zambrano Program 124/MTAI/22+2 种基金and PID2020-113320RB-I00)Agency for Management of University and Research Grants,Regional Government of Catalonia,Spain(2021-SGR-00900)Catalan Institution for Research and Advanced Studies(ICREA).
文摘Background Sperm cryopreservation is widely used in the cattle industry,as it allows for disassociating the localiza-tion of sires and the collection of semen from the timing of artificial insemination.While freeze-thawing is known to impair sperm DNA integrity,whether the damage induced consists of single-(SSB)or double-strand breaks(DSB)has not been determined.In addition,no previous study has addressed if DNA breaks preferentially reside in specific genome regions such as those forming the toroid linker regions,or are rather spread throughout the regions linked to protamines.The main aim of the present work,therefore,was to elucidate the type and localization of the DNA damage generated by cryopreservation and to evaluate its impact on artificial insemination outcomes in cattle.Results The incidence of SSB and DSB was evaluated in 12 ejaculates before and after cryopreservation with the Comet assay,and the localization of the DNA breaks was assessed using pulsed-field gel electrophoresis(PFGE).Before cryopreservation,the incidence of SSB was 10.99%±4.62%and involved 20.56%±3.04%of sperm cells,whereas these figures significantly(P<0.0001)increased up to 34.11%±3.48%and 53.36%±11.00%in frozen-thawed sperm.In contrast,no significant differences in the incidence of DSB were observed(P>0.990)before and after cryopreservation(before:incidence of 13.91%±1.75%of sperm DNA affecting 56.04%±12.49%of sperm cells;after:incidence of 13.55%±1.55%of sperm DNA involving 53.36%±11.00%of sperm cells).Moreover,PFGE revealed that the percentage of sperm DNA fragments whose length was shorter than a toroid(<31.5 kb)was greater(P<0.0001)after(27.00%±4.26%)than before freeze-thawing(15.57%±4.53%).These differences indicated that the DNA breaks induced by cryopreservation affect the regions condensed in protamines,which are structured in toroids.On the other hand,in vivo fertility rates were associated to the incidence of SSB and DSB in frozen-thawed sperm(P=0.032 and P=0.005),but not with the size of the DNA fragments resulting from these breaks(P>0.05).Conclusion Cryopreservation of bovine sperm generates single-strand DNA breaks,which are mainly located in protamine-condensed toroidal regions.The incidence of DNA breaks in cryopreserved sperm has an impact on cat-tle fertility,regardless of the size of generated fragments.
基金funded by National Natural Science Foundation of China(NSFC)(Nos.12075053,11505021 and 11975068)by National Key R&D Program of China(No.2022YFE 03060002)+1 种基金by Fundamental Research Funds for the Central Universities(No.2232024G-10)supported by the U.S.DoE Office of Science(No.DE-FG02–95ER54309)。
文摘Toroidal torques,generated by the resonant magnetic perturbation(RMP)and acting on the plasma column,are numerically systematically investigated for an ITER baseline scenario.The neoclassical toroidal viscosity(NTV),in particular the resonant portion,is found to provide the dominant contribution to the total toroidal torque under the slow plasma flow regime in ITER.While the electromagnetic torque always opposes the plasma flow,the toroidal torque associated with the Reynolds stress enhances the plasma flow independent of the flow direction.A peculiar double-peak structure for the net NTV torque is robustly computed for ITER,as the toroidal rotation frequency is scanned near the zero value.This structure is found to be ultimately due to a non-monotonic behavior of the wave-particle resonance integral(over the particle pitch angle)in the superbanana plateau NTV regime in ITER.These findings are qualitatively insensitive to variations of a range of factors including the wall resistivity,the plasma pedestal flow and the assumed frequency of the rotating RMP field.
文摘在[4]和[5]中已经研究了sim p ly-laced型T oro idal李代数的顶点表示,[6]文据此给出了Bl型T oro idal李代数顶点表示的构造.受[6]文启发,本文给出了G2型T oro idal李代数的顶点表示的构造,这种构造方式与D(41)的D ynk in图的顶点粘合和一个2上循环有着紧密联系.
文摘We will give an overview of results obtained by our reactive fluid model. It is characterised as a fluid model where all moments with sources in the experiment are kept. Furthermore, full account is taken for the highest moments appearing in unexpanded denominators also including full toroidicity. It has been demonstrated that the strength of zonal flows is dramatically larger in reactive fluid closures than in those which involve dissipation. This gives a direct connection between the fluid closure and the level of excitation of turbulence. This is because zonal flows are needed to absorb the inverse cascade in quasi 2D turbulence. This also explains the similarity in structure of the transport coefficients in our model with a reactive closure in the energy equation and models which have a reactive closure because of zero ion temperature such as the Hasegawa-Wakatani model. Our exact reactive closure unifies several well-known features of tokamak experiments such as the L-H transition, internal transport barriers and the nonlinear Dimits upshift of the critical gradient for onset of transport. It also gives transport of the same level as that in nonlinear gyrokinetic codes. Since these include the kinetic resonance this confirms the validity of the thermodynamic properties of our model. Furthermore, we can show that while a strongly nonlinear model is needed in kinetic theory a quasilinear model is sufficient in the fluid description. Thus our quasilinear fluid model will be adequate for treating all relevant problems in bulk transport. This is finally confirmed by the reproduction by the model of the experimental power scaling of the confinement time Te ~ P-2/3. This confirms the validity of our reactive fluid model. This also gives credibility to our ITER simulations including the H-mode barrier. A new result is here, that alpha heating strongly reduces the slope of the H-mode barrier. This should significantly reduce the effects of ELM＇s.
文摘By employing dynamic Monte Carlo simulations, we investigate a coil-to-toroid transition of self-attractive semiflexible polymers and the spatial distributions of nanoparticles in self- attractive semiflexible polymer/nanoparticle composites. The conformation of self-attractive semiflexible polymers depends on bending energy and self-attractive interactions between monomers in polymer chains. A three-stage process of toroid formation for self-attractive semiflexible chains is shown： several isolated toroids, a loose toroid structure, and a compact toroid structure. Utilizing the compact toroid conformations of self-attractive semiflexible chains, we can control effectively the spatial distributions of nanoparticles in self-attractive semiflexible polymer nanocomposites, and an unconventional toroid structure of nanoparti- cles is observed.
文摘This paper aims to study the oscillation of a sparkgenerated submerged bubble located near or inside a circular aperture made in a flat plate using high-speed visualization technique. In the case of a bubble oscillating near an aperture the initial free surface of the water was set at the bottom surface of the plate. The effects of aperture size and bubblefree surface distance on the bubble behavior as well as on the ensuing droplet dynamics are investigated. It was found that the direction of the bubble reentrant jet was towards the aperture or away from it respectively when the normalized aperture size was smaller or greater than a certain critical value. In addition, a toroidal vortex ring was observed to form, which rotated inwards as it moved away from the aperture. It was also found that if the bubble was incepted at a distance sufficiently away from a supercritical size aperture a single droplet could be produced. In the case of a bub- ble initiated in the middle of a circular aperture submerged just beneath the water free surface, the bubble was found to take the shape of an ellipsoid during its expansion. Then a reentrant jet was initiated and pierced the bubble from its top side.
文摘On the basis of the principle of dieless hydrobulging technology, a novel hydrobulging technology for manufacturinghyperbolic plates is proposed. First, a toroidal pipe elbow or a partial toroidal pipe elbow is formed, then the single hydrobulgedstructure can be cut up into some desired plates. It is proved by experiments and finite element simulation that manufacturingtechnology of hyperbolic plates adopting integrated hydrobulging forming technology is feasible.
基金the National Natural Science Foundation of China(10272032 and 10672043).
文摘A level set method of non-uniform grids is used to simulate the whole evolution of a cavitation bubble, including its growth, collapse and rebound near a rigid wall. Single-phase Navier-Stokes equation in the liquid region is solved by MAC projection algorithm combined with second-order ENO scheme for the advection terms. The moving inter-face is captured by the level set function, and the interface velocity is resolved by ＂one-side＂ velocity extension from the liquid region to the bubble region, complementing the second-order weighted least squares method across the interface and projection inside bubble. The use of non-uniform grid overcomes the difficulty caused by the large computational domain and very small bubble size. The computation is very stable without suffering from large flow-field gradients, and the results are in good agreements with other studies. The bubble interface kinematics, dynamics and its effect on the wall are highlighted, which shows that the code can effectively capture the ＂shock wave＂-like pressure and velocity at jet impact, toroidal bubble, and complicated pressure structure with peak, plateau and valley in the later stage of bubble oscillating.
基金the Ford-NSFC Foundation of China (No. 50122151).
文摘The most general contact in mechanical transmission is the elliptical one. In particular, a toroidal continuously variable transmission (CVT) has an elliptical shape in the contact area under the elastohydrodynamic lubrication regime, where the shearing of the fluid subjects to high contact stress and transmits the power. Many parameters affect the contact service performance of the half toroidal CVT, which include the properties of the contacting material (Young抯 modulus), operating parameters (input torque and maximum traction coefficient) and geometrical parameters (aspect cavity ratio, curvature ratio and half cone angle of the power roller). In this paper, the contacts between the input disk, the power roller and the output disk are formulated using the classical Hertzian contact theory. Based on the formulated equations, different system parameters, which affect the maximum Hertzian stresses, are compared. The comparative results will provide some observations of the relations between the maximum Hertzian stresses and transmission ratios in the form of graphs. These graphs give useful information for designer to know the maximum Hertzian stress during operation in such systems.
