Dental caries is primarily caused by cariogenic bacteria metabolizing carbohydrates to produce acidic substances that erode the dental hard tissues.Traditional remineralization treatments often have limited efficacy d...Dental caries is primarily caused by cariogenic bacteria metabolizing carbohydrates to produce acidic substances that erode the dental hard tissues.Traditional remineralization treatments often have limited efficacy due to their lack of antibacterial activity.According to the Interrupting Dental Caries(IDC)theory,ideal caries-preventive materials should possess both antibacterial and remineralizing properties.Furthermore,effective adhesion to dental surfaces is crucial.Inspired by the wet adhesion properties of the salivary acquired pellicle,we developed a multifunctional gargle named Ce6@PDN-SAP(CP-SAP).This formulation employed peptide dendrimer nanogels(PDN)as a carrier for the photosensitizer Ce6,further functionalized with saliva-acquired peptide(SAP)to confer wet adhesion properties.CP-SAP rapidly adhered to the dental surface and remained effective for extended periods.Upon laser irradiation,Ce6 generated reactive oxygen species(ROS),disrupting bacterial outer membrane integrity,causing protein leakage,and reducing ATP levels,thereby achieving potent antibacterial effects.Following this,PDN and SAP acted as nucleation templates to promote in situ remineralization of demineralized dental hard tissues.In vivo studies using rat models demonstrated that CP-SAP provided significantly superior caries-preventive effects compared to chlorhexidine gargle.In conclusion,CP-SAP,as an innovative approach grounded in the IDC theory,holds great promise for the prevention and treatment of dental caries.展开更多
Dental caries is one of the most prevalent human diseases resulting from tooth demineralization caused by acid production of bacteria plaque.It remains challenges for current practice to specifically identify,interven...Dental caries is one of the most prevalent human diseases resulting from tooth demineralization caused by acid production of bacteria plaque.It remains challenges for current practice to specifically identify,intervene and interrupt the development of caries while restoring defects.In this study,inspired by natural dental plaque,a stimuli-responsive multidrug delivery system(PMs@NaF-SAP)has been developed to prevent tooth decay and promote enamel restoration.Classic spherical core-shell structures of micelles dual-loaded with antibacterial and restorative agents are self-assembled into bacteria-responsive multidrug delivery system based on the pH-cleavable boronate ester bond,followed by conjugation with salivary-acquired peptide(SAP)to endow the nanoparticle with strong adhesion to tooth enamel.The constructed PMs@NaF-SAP specifically adheres to tooth,identifies cariogenic conditions and intelligently releases drugs at acidic pH,thereby providing antibacterial adhesion and cariogenic biofilm resistance,and restoring the microarchitecture and mechanical properties of demineralized teeth.Topical treatment with PMs@NaF-SAP effectively diminishes the onset and severity of caries without impacting oral microbiota diversity or surrounding mucosal tissues.These findings demonstrate this novel nanotherapy has potential as a promising biomedical application for caries prevention and tooth defect restoration while resisting biofilm-associated diseases in a controlled manner activated by pathological bacteria.展开更多
Caries is one of the most prevalent human diseases,resulting from demineralization of tooth hard tissue caused by acids produced from bacteria,and can progress to pulpal inflammation.Filling restoration with dental re...Caries is one of the most prevalent human diseases,resulting from demineralization of tooth hard tissue caused by acids produced from bacteria,and can progress to pulpal inflammation.Filling restoration with dental resin composites(DRCs)is currently the most common treatment for caries.However,existing DRCs suffer from low fracture strength and lack comprehensive anti-caries bioactivity including remineralization,pulp protection,and anti-cariogenic bacteria effects.In this study,inspired by plant roots’ability to stabilize and improve soil,fluorinated urchin-like hydroxyapatite(FUHA)with a three-dimensional whisker structure and bioactive components of calcium,phosphorus,and fluorine was designed and synthesized by a dynamic self-assembly method.Furthermore,versatile FUHA particles with different loading fractions were used as functional fillers to fabricate methacrylate-based DRCs,where the urchin-like hydroxyapatite(UHA)filled DRCs and commercial DRCs(Z350XT and BEAUTIFIL II)served as the control groups.The results demonstrated that FUHA with 50 wt%loading in resin matrix endowed DRC(F5)with excellent physicochemical properties,dentin remineralization property,cell viability,promotion of dental pulp stem cells mineralization,and antibacterial properties.Meanwhile,F5 also presented good clinical handling and aesthetic characteristics.Therefore,structure/functional-integrated FUHA filled DRCs have potential as a promising strategy for tooth restoration and anti-caries bioactivity.展开更多
基金supported by the National Natural Science Foundation of China(82170950,82272150)National Health Commission of the People’s Republic of China(WKJ-ZJ-2214)Wenzhou Basic Scientific Research Project(Grant No.Y20240323).
文摘Dental caries is primarily caused by cariogenic bacteria metabolizing carbohydrates to produce acidic substances that erode the dental hard tissues.Traditional remineralization treatments often have limited efficacy due to their lack of antibacterial activity.According to the Interrupting Dental Caries(IDC)theory,ideal caries-preventive materials should possess both antibacterial and remineralizing properties.Furthermore,effective adhesion to dental surfaces is crucial.Inspired by the wet adhesion properties of the salivary acquired pellicle,we developed a multifunctional gargle named Ce6@PDN-SAP(CP-SAP).This formulation employed peptide dendrimer nanogels(PDN)as a carrier for the photosensitizer Ce6,further functionalized with saliva-acquired peptide(SAP)to confer wet adhesion properties.CP-SAP rapidly adhered to the dental surface and remained effective for extended periods.Upon laser irradiation,Ce6 generated reactive oxygen species(ROS),disrupting bacterial outer membrane integrity,causing protein leakage,and reducing ATP levels,thereby achieving potent antibacterial effects.Following this,PDN and SAP acted as nucleation templates to promote in situ remineralization of demineralized dental hard tissues.In vivo studies using rat models demonstrated that CP-SAP provided significantly superior caries-preventive effects compared to chlorhexidine gargle.In conclusion,CP-SAP,as an innovative approach grounded in the IDC theory,holds great promise for the prevention and treatment of dental caries.
基金supported by the National Natural Science Foundation of China(No.82001106,81970918,81901043).
文摘Dental caries is one of the most prevalent human diseases resulting from tooth demineralization caused by acid production of bacteria plaque.It remains challenges for current practice to specifically identify,intervene and interrupt the development of caries while restoring defects.In this study,inspired by natural dental plaque,a stimuli-responsive multidrug delivery system(PMs@NaF-SAP)has been developed to prevent tooth decay and promote enamel restoration.Classic spherical core-shell structures of micelles dual-loaded with antibacterial and restorative agents are self-assembled into bacteria-responsive multidrug delivery system based on the pH-cleavable boronate ester bond,followed by conjugation with salivary-acquired peptide(SAP)to endow the nanoparticle with strong adhesion to tooth enamel.The constructed PMs@NaF-SAP specifically adheres to tooth,identifies cariogenic conditions and intelligently releases drugs at acidic pH,thereby providing antibacterial adhesion and cariogenic biofilm resistance,and restoring the microarchitecture and mechanical properties of demineralized teeth.Topical treatment with PMs@NaF-SAP effectively diminishes the onset and severity of caries without impacting oral microbiota diversity or surrounding mucosal tissues.These findings demonstrate this novel nanotherapy has potential as a promising biomedical application for caries prevention and tooth defect restoration while resisting biofilm-associated diseases in a controlled manner activated by pathological bacteria.
基金the support from the National Natural Science Foundation of China(Grant Nos.82201115,81921002)China Postdoctoral Science Foundation(No.2021M702166)the Innovative Research Team of High-level Local Universities in Shanghai,Oral and Maxillofacial Regeneration and Functional Restoration.
文摘Caries is one of the most prevalent human diseases,resulting from demineralization of tooth hard tissue caused by acids produced from bacteria,and can progress to pulpal inflammation.Filling restoration with dental resin composites(DRCs)is currently the most common treatment for caries.However,existing DRCs suffer from low fracture strength and lack comprehensive anti-caries bioactivity including remineralization,pulp protection,and anti-cariogenic bacteria effects.In this study,inspired by plant roots’ability to stabilize and improve soil,fluorinated urchin-like hydroxyapatite(FUHA)with a three-dimensional whisker structure and bioactive components of calcium,phosphorus,and fluorine was designed and synthesized by a dynamic self-assembly method.Furthermore,versatile FUHA particles with different loading fractions were used as functional fillers to fabricate methacrylate-based DRCs,where the urchin-like hydroxyapatite(UHA)filled DRCs and commercial DRCs(Z350XT and BEAUTIFIL II)served as the control groups.The results demonstrated that FUHA with 50 wt%loading in resin matrix endowed DRC(F5)with excellent physicochemical properties,dentin remineralization property,cell viability,promotion of dental pulp stem cells mineralization,and antibacterial properties.Meanwhile,F5 also presented good clinical handling and aesthetic characteristics.Therefore,structure/functional-integrated FUHA filled DRCs have potential as a promising strategy for tooth restoration and anti-caries bioactivity.
