Janus hydrogels have recently emerged as promising bioadhesives for efficient wet-tissue adhesion and anti-postoperative adhesion.However,existing Janus hydrogel adhesives normally need varied chemical designs of diff...Janus hydrogels have recently emerged as promising bioadhesives for efficient wet-tissue adhesion and anti-postoperative adhesion.However,existing Janus hydrogel adhesives normally need varied chemical designs of different layers to achieve asymmetric adhesive/anti-adhesive properties on either side.Here,we present a new strategy to construct an adhesive/anti-adhesive Janus hydrogel tissue patch accomplished by switching the charge-balance of the hydrogel layers with similar compositions(anionic carboxyl polymer and cationicε-polylysine,EPL).The bottom layer(AL)is formed under acidic condition(pH 2.85),featuring abundant-COOH and-NH3+residues,which provide rapid&robust adhesion to diverse wet tissues(up to 100.4 kPa)with high bursting pressure(362.5 mmHg),while the top layer(MLT)is formed under neutral condition,achieving a balanced charge between-COOH/-NH2 and-COO/NH3+groups,which mimic the overall electroneutral structure of zwitterionic materials for efficient anti-postoperative tissue adhesion(up to 6 weeks).Further in vivo studies validated that the integrated AL/MLT hydrogel patch is biodegradable(within 10 weeks),exhibits broad-spectrum antibacterial activity(up to 99.8%),and outperforms the commercial fibrin gel in sutureless wound sealing,rat gastric tissue repair,and anti-postoperative adhesion.This strategy may open a new avenue to develop adhesive/anti-adhesive Janus bioadhesives for efficient non-invasive internal tissue sealing and promoted wound healing.展开更多
The use of tissue adhesive patches provides a promising therapeutic approach for sutureless repair of soft tissue injuries.However,existing tissue adhesive patches are confronted with serious challenges for clinical a...The use of tissue adhesive patches provides a promising therapeutic approach for sutureless repair of soft tissue injuries.However,existing tissue adhesive patches are confronted with serious challenges for clinical applications in the soft tissue environments with biological fluids and dynamic movements.Either their mechanical toughness does not match that of soft tissues,or they fail to establish effective interfacial bonding with tissues in wet conditions.The imbalance between the mechanical cohesion and interfacial adhesion of existing tissue adhesive patches severely restricts their conformal integration with wet surfaces of soft tissues in dynamic biological environments,leading to adhesion failure in clinical applications.Here,this study reports the design,fabrication,and preclinical therapeutic performance of a dual-layer silk-based adhesive patch(named SF patch)that quickly and conformally adheres to various soft tissues regardless of surrounding biological environments.The intimate microscopic structural connection between the highly tough hydrogel matrix layer and thin bioadhesive layer contributes to high mechanical cohesion and robust interfacial adhesion properties of the SF patch,thereby enabling sufficient integration with wet surfaces of soft tissues to withstand the interference of dynamic tissue movements.Ex vivo porcine and in vivo rat models validate its therapeutic efficacy for sutureless sealing and repair of gastrointestinal defects and peripheral nerve injuries.This SF patch is potentially valuable for clinical applications towards internal soft-tissue repair and functional reconstruction.展开更多
Pressure ulcers(PUs)are common skin injuries known for their high morbidity,rapid onset,susceptibility to infection,and challenging healing process.One potential therapy for PUs is cell-based therapy using mesenchymal...Pressure ulcers(PUs)are common skin injuries known for their high morbidity,rapid onset,susceptibility to infection,and challenging healing process.One potential therapy for PUs is cell-based therapy using mesenchymal stem cells(MSCs).However,poor survival and low cell retention of MSCs on skin lesions limit their therapeutic effects and applications.In this study,we prepared an extracellular matrix(dECM)hydrogel decellularized from the human umbilical cord(UC).A patch composed of UC-dECM and UC-MSCs was employed in the treatment of PUs in C57BL/6 mice.Our results indicate that the UC-dECM hydrogel effectively sustains cell viability,enhances the stemness-related gene expression in UC-MSCs,and promotes human umbilical vein endothelial cells(HUVECs)migration and angiogenesis.Compared to the groups treated with the patch containing only UC-dECM,injection of UC-MSCs or gauze dressing,the patch combining UC-dECM hydrogel with UC-MSCs significantly accelerated PU healing.This positive outcome can be attributed to the promotion of tissue re-epithelialization,collagen deposition,angiogenesis,and inflammation inhibition.Our results suggest that the composite patch,comprised of UC-dECM hydrogel and UC-MSCs,may be a promising therapeutic approach for PU treatment.展开更多
基金supports from the Six Talent Peaks Project in Jiangsu Province(SWYY-060)Changzhou City Major Technology De-mand"Unveiling and Leading"Science and Technology Research Project(S11090B42215)the Postdoctoral Fellowship Program of CPSF under Grant Number GZC20231142.
文摘Janus hydrogels have recently emerged as promising bioadhesives for efficient wet-tissue adhesion and anti-postoperative adhesion.However,existing Janus hydrogel adhesives normally need varied chemical designs of different layers to achieve asymmetric adhesive/anti-adhesive properties on either side.Here,we present a new strategy to construct an adhesive/anti-adhesive Janus hydrogel tissue patch accomplished by switching the charge-balance of the hydrogel layers with similar compositions(anionic carboxyl polymer and cationicε-polylysine,EPL).The bottom layer(AL)is formed under acidic condition(pH 2.85),featuring abundant-COOH and-NH3+residues,which provide rapid&robust adhesion to diverse wet tissues(up to 100.4 kPa)with high bursting pressure(362.5 mmHg),while the top layer(MLT)is formed under neutral condition,achieving a balanced charge between-COOH/-NH2 and-COO/NH3+groups,which mimic the overall electroneutral structure of zwitterionic materials for efficient anti-postoperative tissue adhesion(up to 6 weeks).Further in vivo studies validated that the integrated AL/MLT hydrogel patch is biodegradable(within 10 weeks),exhibits broad-spectrum antibacterial activity(up to 99.8%),and outperforms the commercial fibrin gel in sutureless wound sealing,rat gastric tissue repair,and anti-postoperative adhesion.This strategy may open a new avenue to develop adhesive/anti-adhesive Janus bioadhesives for efficient non-invasive internal tissue sealing and promoted wound healing.
基金supported by the National Natural Science Foundation of China(Grant No.22175038,22027805,22334004,22421002)the National Key Research&Development Program of China(Grant No.2020YFA0709900)the Natural Science Foundation of Fujian Province of China(Grant No.2021J01610).
文摘The use of tissue adhesive patches provides a promising therapeutic approach for sutureless repair of soft tissue injuries.However,existing tissue adhesive patches are confronted with serious challenges for clinical applications in the soft tissue environments with biological fluids and dynamic movements.Either their mechanical toughness does not match that of soft tissues,or they fail to establish effective interfacial bonding with tissues in wet conditions.The imbalance between the mechanical cohesion and interfacial adhesion of existing tissue adhesive patches severely restricts their conformal integration with wet surfaces of soft tissues in dynamic biological environments,leading to adhesion failure in clinical applications.Here,this study reports the design,fabrication,and preclinical therapeutic performance of a dual-layer silk-based adhesive patch(named SF patch)that quickly and conformally adheres to various soft tissues regardless of surrounding biological environments.The intimate microscopic structural connection between the highly tough hydrogel matrix layer and thin bioadhesive layer contributes to high mechanical cohesion and robust interfacial adhesion properties of the SF patch,thereby enabling sufficient integration with wet surfaces of soft tissues to withstand the interference of dynamic tissue movements.Ex vivo porcine and in vivo rat models validate its therapeutic efficacy for sutureless sealing and repair of gastrointestinal defects and peripheral nerve injuries.This SF patch is potentially valuable for clinical applications towards internal soft-tissue repair and functional reconstruction.
基金Funded by the Guangdong Basic and Applied Basic Research Foundation(2023A1515012452,2021B1515230011)the Science and Technology Program of Guangzhou(2024B03J0022,201803010086)。
文摘Pressure ulcers(PUs)are common skin injuries known for their high morbidity,rapid onset,susceptibility to infection,and challenging healing process.One potential therapy for PUs is cell-based therapy using mesenchymal stem cells(MSCs).However,poor survival and low cell retention of MSCs on skin lesions limit their therapeutic effects and applications.In this study,we prepared an extracellular matrix(dECM)hydrogel decellularized from the human umbilical cord(UC).A patch composed of UC-dECM and UC-MSCs was employed in the treatment of PUs in C57BL/6 mice.Our results indicate that the UC-dECM hydrogel effectively sustains cell viability,enhances the stemness-related gene expression in UC-MSCs,and promotes human umbilical vein endothelial cells(HUVECs)migration and angiogenesis.Compared to the groups treated with the patch containing only UC-dECM,injection of UC-MSCs or gauze dressing,the patch combining UC-dECM hydrogel with UC-MSCs significantly accelerated PU healing.This positive outcome can be attributed to the promotion of tissue re-epithelialization,collagen deposition,angiogenesis,and inflammation inhibition.Our results suggest that the composite patch,comprised of UC-dECM hydrogel and UC-MSCs,may be a promising therapeutic approach for PU treatment.
