The assembling of a coating of time-resolved fluorescent chelator BSPDA （ abbreviated for 4, 7-bis （ sulfhydrylphenyl）-1, 10-phenanthroline-2, 9-dicarboxylic acid） onto a nano-gold layer was demonstrated. First, B...The assembling of a coating of time-resolved fluorescent chelator BSPDA （ abbreviated for 4, 7-bis （ sulfhydrylphenyl）-1, 10-phenanthroline-2, 9-dicarboxylic acid） onto a nano-gold layer was demonstrated. First, BSPDA was synthesized by simple procedures, and then an approach was developed to immobilize BSPDA onto the nano-gold layer deposited on a silane modified glass substrate, whereby europium ion （Ⅲ, Eu^3＋ ） was captured and released owing to the interactive process of complexation and dissociation between BSPDA functionalized coating and Eu^3＋ solution. The fluorescence spectra and related lifetimes were determined. Also, the BSPDA functionalized coating＇s specific complexation with Eu^3＋ on the BSPDA assembly layer and the nonspecific adsorption of Eu^3＋ on the nano-gold layer were compared. These results allowed a selective complexation of Eu^3＋ by assembling a BSPDA chelating layer on the nano-gold layer; thus, a tunable time-resolved fluorescent layer was covalently attached, The results of the nanoparticle assembling and probing （or labeling） processes to specific bio-systems were very interesting and had significant implications to time-resolved-fluorescence-based detection on biosensor surfaces such as DNA chip and to arrayed light display devices.展开更多
[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PP...[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PPR.[Methods]Soluble N protein and NH fusion protein were successfully obtained in an Escherichia coli expression system by optimizing E.coli codon and expression conditions.Furthermore,based on purified soluble N protein and NH fusion protein,a double-antigen sandwich time-resolved fluorescence immunoassay method for detection of peste des petits ruminants virus(PPRV)was established.[Results]The method has high sensitivity and specificity and can specifically detect the antibody against PPRV in sheep serum,and it has no cross reaction with other related diseases.The method was used to detect 292 clinical samples,and compared with French IDVET competition ELISA kit.The coincidence rates of positive samples and negative samples from the two kinds of test kits were 92.47%and 97.26%,respectively,and the overall coincidence rate was 94.86%.The intra-group and inter-group coefficients of variation in the repeatability test were less than 10%.[Conclusions]Compared with the traditional ELISA method,the double-antigen sandwich time-resolved fluorescence immunoassay for detection of PPRV has equivalent sensitivity and specificity,and simple and rapid operation,and thus high application and popularization value.展开更多
Timefluorescence decays were first performed on hematoporphyrin derivative laser No.3 in buffer at different micelles concentration.The flu.orescence lifetime constants,measured with a time-correlated single photon co...Timefluorescence decays were first performed on hematoporphyrin derivative laser No.3 in buffer at different micelles concentration.The flu.orescence lifetime constants,measured with a time-correlated single photon counting system;could be fitted by two or three exponential components with different relative peak amplitudes depending on the observation wavelength^thus indicating the presence of different emitting molecular species and/or of molecular species in different aggregational states.展开更多
Using a picosecond excitation source and a time-resolved streak camera detection system,the time-resolved fluorescence spectra of purified C_(60) have been recorded from toluene solution at room temperature.A fluoresc...Using a picosecond excitation source and a time-resolved streak camera detection system,the time-resolved fluorescence spectra of purified C_(60) have been recorded from toluene solution at room temperature.A fluorescence peak at about 730nm was detected and its lifetime was directly determined to be 1.1±0.1 ns.Two fluorescence peaks obtained from the mixture of C_(60) and C_(70) at 676 and 696nm were mainly related to C_(70) content.展开更多
Accurate quantification of interleukin-6(IL6),a biomarker central to sepsis and cytokine release syndrome,is essential for assessing disease severity.Here,we present a high-performance lateral flow assay(LFA)that leve...Accurate quantification of interleukin-6(IL6),a biomarker central to sepsis and cytokine release syndrome,is essential for assessing disease severity.Here,we present a high-performance lateral flow assay(LFA)that leverages a novel conjugate:europium nanoparticles(EuNPs)labels linked via tetrahedral DNA frameworks(TDFs).The TDF precisely controls antibody orientation,minimizes nonspecific binding,and improves conjugate stability.Combined with the strong,time-resolved fluorescence of EuNPs,this design achieves a broad dynamic range and preserves linearity at high analyte concentrations.The platform quantitatively detects IL6 from 0 to 5000 pg/mL within 10 min,showing excellent agreement with reference methods.This DNA-nanostructureenhanced approach provides a robust and portable point-of-care testing strategy for critical clinical decisionmaking.展开更多
An enhancement method of rapid lifetime determination is proposed for time-resolved fluorescence imaging to discriminate substances with approximate fluorescence lifetime in forensic examination. In the method, an ima...An enhancement method of rapid lifetime determination is proposed for time-resolved fluorescence imaging to discriminate substances with approximate fluorescence lifetime in forensic examination. In the method, an image-exclusive-OR treatment with filter threshold adaptively chosen is presented to extract the region of interest from dual-gated fluorescence intensity images, and then the fluorescence lifetime image is reconstructed based on the rapid lifetime determination algorithm. Furthermore, a maximum and minimum threshold filtering is developed to automatically realize visualization enhancement of the lifetime image. In proof experiments, compared with traditional fluorescence intensity imaging and rapid lifetime determination method, the proposed method automatically distinguishes altered and obliterated documents written by two brands of highlighters with the same color and close fluorescence lifetime.展开更多
In recent years,phosphodiesterase type 5 enzyme inhibitors,including tadalafil,sildenafil,and their analogues have emerged as illegal ingredients in dietary supplements.It can cause serious clinical side effects in co...In recent years,phosphodiesterase type 5 enzyme inhibitors,including tadalafil,sildenafil,and their analogues have emerged as illegal ingredients in dietary supplements.It can cause serious clinical side effects in consumers,resulting in diverse symptoms.To rapidly screen illegal drugs in dietary supplements,a dual-labeled probe time-resolved fluorescence immunochromatography assay(DL-TRFICA)based on two broad-spectrum antibodies labeled with time-resolved fluorescent microspheres was established for the simultaneous qualitative and quantitative detection of nine sildenafil-like and three tadalafil-like adulterants.The half-maximal inhibitory concentration(IC50)of sildenafil-like and tadalafil-like adulterants were 2.49-5.88 and 2.15-2.78 ng mL^(-1),the limit of detection were 0.08-1.66 and 0.59-1.85 ng mL^(-1),and the visualization value(cut-off value)were 31.25-250 and 50-62.50 ng mL^(-1),respectively.Notably,the potential practical applications of DL-TRFICA evaluated by conducting a quantitative evaluation of sildenafil and tadalafil adulterants in dietary supplement samples(herbal spirit drinks,healthcare oral liquids,and herbal medicine pills)demonstrated a recovery rate ranging from 80.0%to 125.8%with a coefficient of variation of less than 15%,which concluded that the DL-TRFICA approach could be used in real sample detection.展开更多
Fumonisins(FBs)are mycotoxins primarily synthesized by Fusarium moniliformis.Among these,FB1 exhibits not only high toxicity towards humans and animals but also carcinogenic properties.The global prevalence of FB1 con...Fumonisins(FBs)are mycotoxins primarily synthesized by Fusarium moniliformis.Among these,FB1 exhibits not only high toxicity towards humans and animals but also carcinogenic properties.The global prevalence of FB1 contamination in cereals and related products,particularly maize,is alarmingly significant.Consequently,the accurate determination of FB1 levels in cereals holds immense importance.In this study,highly sensitive monoclonal antibodies specifically targeting FB1 were prepared and utilized for the establishment of a time-resolved fluorescence immunochromatographic assay(TRFIC)to detect FB1.The parameters of antibody labeling with time-resolved fluorescent microspheres were optimized.The detection time was significantly reduced to 6 min.The limits of detection(LOD)for corn,rice,and feed were determined as 0.496-0.844μg/kg,and the quantification(LOQ)was 0.788-1.322μg/kg.In addition,an indirect competitive enzyme-linked immunosorbent assay(ic-ELISA)was successfully developed.Under optimized conditions,the half inhibitory concentration(IC50)value for FB1 was determined as 2.137μg/L.A strong correlation between the results obtained from these two methods and HPLC-MS/MS analysis was observed in the same samples tested.In conclusion,both immunological methods developed in this work are highly suitable for rapid FB1 detection in real field samples.展开更多
Objective To investigate the prevalence and clinical significance of the centromere protein-F-like(CENP-F-like)immunofluorescence staining pattern in a large patient cohort and through literature review.Methods We ret...Objective To investigate the prevalence and clinical significance of the centromere protein-F-like(CENP-F-like)immunofluorescence staining pattern in a large patient cohort and through literature review.Methods We retrospectively analyzed antinuclear antibody(ANA)immunofluorescence assay results from 191274 patients at West China Hospital of Sichuan University between March 2018 and November 2020.Specific immunological markers were tested in sera with CENP-F-like patterns.Additionally,a narrative review of seven relevant studies was performed for comparison.Results In Southwest China,ANA positivity was found in 32.09%of patients,with the CENP-F-like pattern detected in 0.015%of all cases and 0.05%of ANA-positive individuals.The CENP-F-like pattern appeared predominantly at titers≥1∶320,most often in isolation(68.97%),but also mixed with cytoplasmic speckled patterns.Patients with cancers accounted for the highest proportion(31.03%),including solid tumors and hematologic malignancies.Metastasis was observed in patients with solid tumors,while graft-versus-host disease(GVHD)occurred in those with hematologic malignancies post-transplantation.Autoimmune diseases(AIDs)were diagnosed in 20.69%of cases,all showing disease-specific autoantibodies.These findings were broadly consistent with previous reports and suggest a possible association between the CENP-F-like pattern and malignancies.Conclusion The CENP-F-like pattern is rare in ANA tests but may be associated with clinically important conditions,particularly cancers and AIDs.The occurrence of metastasis and GVHD in patients with this pattern highlights its potential clinical relevance,and concurrent autoantibodies may assist in diagnosing AIDs.展开更多
Cyclo[n]Thiophenes(CnTs)are a distinctive class of π-conjugated macrocyclic molecules that have attracted growing attention owing to their structural aesthetics and organic electronic characteristics.However,the deve...Cyclo[n]Thiophenes(CnTs)are a distinctive class of π-conjugated macrocyclic molecules that have attracted growing attention owing to their structural aesthetics and organic electronic characteristics.However,the development of CnTs has been largely impeded by inefficient synthetic route.In this work,we employ a bridge strategy using bipyridine as bridge to link two quaterthiophene units resulting in Φ-shaped bicyclosystem.This strain-retaining approach improves the synthesis efficiency of the macrocycles.Two new macrocyclic molecules,(4T-2hexyl-2Me)_(2)-DPBP and(4T-2hexyl)_(2)-DPBP,were successfully synthesized in total yield 17%and 16%,respectively.Single-crystal structure of(4T-2hexyl-2Me)_(2)-DPBP reveals that the bipyridine bridge is orthogonally strapped by two quaterthiophene units.Notably,both compounds exhibit aggregation-induced emission enhancement(AIEE)behavior-an unprecedented feature among CnT-based macrocycles.Theoretical calculations reveal that this AIE phenomenon originates from the restriction of intramolecular motion(RIM)in the aggregated state,which suppresses the non-radiative decay channels.These results demonstrate a generalized strategy for the synthesis of functionalπ-conjugated macrocyclic molecules based fluorescent materials.展开更多
Over the past ten years,numerous papers have been published on the use of indocyanine green(ICG)fluorescence in liver surgery for hepatocellular carcinoma(HCC).There are many different applications.The first involves ...Over the past ten years,numerous papers have been published on the use of indocyanine green(ICG)fluorescence in liver surgery for hepatocellular carcinoma(HCC).There are many different applications.The first involves targeting superficial tumors in patients with macronodular cirrhosis and an irregular liver surface.In a minimally invasive setting,the lack of tactile feedback on the hepatic surface makes detecting subcapsular HCC with ultrasound alone challenging.ICG fusion images can mimic the tactile feedback of the hand and act as an ultrasound booster.ICG fluorescence can be used to evaluate tumor residues after minimally invasive thermal ablation.ICG fluorescence imaging can also be used to identify the grade of HCC early on and evaluate the microinvasive component.展开更多
Based on 4'-(1H-tetrazol-5-yl)-[1,1'-biphenyl]-2,4,6-tricarboxylic acid(H_(4)bta)ligand,zinc metal-organic framework(Zn-MOF):{[Zn_(2)(bta)(bpy)_(2)(H_(2)O)]·1.5H_(2)O}_(n)(bpy=2,2'-bipyridine)was desi...Based on 4'-(1H-tetrazol-5-yl)-[1,1'-biphenyl]-2,4,6-tricarboxylic acid(H_(4)bta)ligand,zinc metal-organic framework(Zn-MOF):{[Zn_(2)(bta)(bpy)_(2)(H_(2)O)]·1.5H_(2)O}_(n)(bpy=2,2'-bipyridine)was designed and synthesized by hydrothermal method.Its structure was characterized by elemental analysis,IR spectra,X-ray single crystal diffraction,etc.The asymmetric unit of Zn-MOF contains two crystallographically independent Zn^(2+)ions.Through the connection of Zn^(2+)ions via H_(4)bta,a 1D double-layer network structure is formed.Adjacent double-layer networks further form a 2D supramolecular network through hydrogen bonding.Notably,Zn-MOF exhibited excellent fluorescence properties and could efficiently and sensitively detect various water pollutants:4-nitrophenol(4-NP),Cu^(2+),and pyrimethanil(Pth).Additionally,the mechanism of fluorescence sensing was investigated.CCDC:2432230.展开更多
The level of glutathione(GSH)is significantly associated with numerous pathological processes,thus,real-time detection of the GSH level is of significance for early diagnosis of GSH-related diseases.Herein,we develope...The level of glutathione(GSH)is significantly associated with numerous pathological processes,thus,real-time detection of the GSH level is of significance for early diagnosis of GSH-related diseases.Herein,we developed in vivo second near-infrared(NIR-II)window fluorescence(FL)and ratiometric photoacoustic(RPA)dual-modality imaging of GSH using a GSH-activatable probe(LET-14).LET-14 was synthesized based on a rhodamine hybrid xanthene skeleton with a FL shielding 2,4-dinitrobenzene sulfonyl group that can be specifically cleaved by GSH,thus resulting in a markedly bathochromic-shift absorption,a 6.5-fold increase in NIR-II FL intensity(FL_(920))and a 13-fold increase in RPA signal(PA_(880)/PA_(705))in vitro.Intriguingly,LET-14 exhibits good selectivity and sensitivity for NIR-II FL and RPA dual-modality imaging of GSH in 4T1 tumor-bearing mouse model.Our findings develop an in vivo detection tool of GSH,which has great potential in the field of cancer diagnosis.展开更多
Accurate phenological information is essential for measuring ecosystem dynamics and carbon uptake.Southwest China is one of the country's largest terrestrial carbon sink regions and plays a crucial role in carbon ...Accurate phenological information is essential for measuring ecosystem dynamics and carbon uptake.Southwest China is one of the country's largest terrestrial carbon sink regions and plays a crucial role in carbon peaking and neutrality.However,its complex terrain,fragile ecosystem,and variable climate challenge carbon sink stability.Vegetation phenology significantly impacts carbon absorption and release,making accurate phenological data essential for understanding carbon sequestration dynamics.The widespread distribution of evergreen forests and their weak seasonal variation in canopy introduce significant uncertainties in extracting phenology using traditional remote sensing information in this region.These limitations can lead to inaccurate assessments of carbon sink dynamics.Therefore,precise phenology extraction and analysis are vital for improving ecosystem dynamics and the carbon cycle in Southwest China.Firstly,we employed different ways to evaluate the ability of solar-induced chlorophyll fluorescence(SIF)and traditional remote sensing information to extract phenology.Secondly,based on SIF,we analyzed the spatial and temporal changes in the start of the growing season(SOS),the end of the growing season(EOS),and the length of the growing season(LOS)from 2001 to 2020.Finally,we systematically analyzed the response of SOS and EOS to five preseason climatic factors.The results showed that(1)SIF outperformed traditional remote sensing information in extracting phenology.(2)Vegetation phenology exhibited significant spatial heterogeneity.Moreover,SOS,EOS,and LOS showed trends of advancement,delay,and extension both overall and across all vegetation types.(3)Precipitation was the main factor influencing SOS,while surface downward solar radiation and mean temperature were the main factors affecting EOS,and the phenology of different vegetation types showed a great difference in response to preseason climate factors.These findings improve our understanding of vegetation phenology and its dynamics over Southwest China.展开更多
Accurately assessing the carbon sequestration capacity of forests is crucial for mitigating climate change.Traditional methods for estimating Gross Primary Productivity(GPP)of vegetation involve significant uncertaint...Accurately assessing the carbon sequestration capacity of forests is crucial for mitigating climate change.Traditional methods for estimating Gross Primary Productivity(GPP)of vegetation involve significant uncertainties.As a novel remote sensing approach,Solar-Induced chlorophyll Fluorescence(SIF)is directly related to photosynthesis and has demonstrated strong correlations with GPP across various ecosystems,climate zones,and spatial scales.Current GPP estimation methods based on SIF include Light Use Efficiency(LUE)models,the SCOPE process models,and the latest mechanistic light response(MLR)models.Future research should focus on improving the mechanistic understanding of SIF-related processes and promoting the integration of multi-source remote sensing data with SIF-based modeling to enhance the accuracy and universality of GPP estimation.展开更多
A series of blue and blue‑green Ir(Ⅲ)complexes has been investigated theoretically to explore their electronic structures,photophysical properties,efficiency roll‑off effect,and thermal activation delayed fluorescenc...A series of blue and blue‑green Ir(Ⅲ)complexes has been investigated theoretically to explore their electronic structures,photophysical properties,efficiency roll‑off effect,and thermal activation delayed fluorescence(TADF)properties.All calculations were performed using density functional theory(DFT)and time‑dependent density functional theory(TDDFT).Calculations for electronic structures,frontier molecular orbital characteristics(which determine the efficiency roll‑off effect of the complexes),and photophysical properties were conducted using the Gaussian 09 software package.The calculation of spin‑orbit coupling matrix elements<T|HSOC|S>,which determine the TADF properties of the complexes,was performed using the ORCA software package.The calculation results show that the auxiliary ligand tetraphenylimidodiphosphinate(tpip),a strong electron‑withdrawing group,can mitigate the efficiency roll‑off effect of the complex.Furthermore,TADF is observed in one of the designed complexes,(F_(3)Phppy)_(2)Ir(tpip),where F_(3)Phppy=2‑[4‑(2,4,6‑trifluorophenyl)phenyl]pyridine.展开更多
Existing methods for tracing water pollution sources typically integrate three-dimensional excitationemission matrix(3D-EEM)fluorescence spectroscopy with similarity-based matching algorithms.However,these approaches ...Existing methods for tracing water pollution sources typically integrate three-dimensional excitationemission matrix(3D-EEM)fluorescence spectroscopy with similarity-based matching algorithms.However,these approaches exhibit high error rates in borderline cases and necessitate expert manual review,which limits scalability and introduces inconsistencies between algorithmic outputs and expert judgment.To address these limitations,we propose a large vision-language model(VLM)designed as an“expert agent”to automatically refine similarity scores,ensuring alignment with expert decisions and overcoming key application bottlenecks.The model consists of two core components:(1)rule-based similarity calculation module generate initial spectral similarity scores,and(2)pre-trained large vision-language model fine-tuned via supervised learning and reinforcement learning with human feedback(RLHF)to emulate expert assessments.To facilitate training and evaluation,we introduce two expert-annotated datasets,Spec1k and SpecReason,which capture both quantitative corrections and qualitative reasoning patterns,allowing the model to emulate expert decision-making processes.Experimental results demonstrate that our method achieves 81.45%source attribution accuracy,38.24%higher than rule-based and machine learning baselines.Real-world deployment further validates its effectiveness.展开更多
The increasing demand for flexible displays and wearable electronics has driven extensive efforts to develop stretchable organic lightemitting diodes(OLEDs).A critical challenge in this field is the creation of emissi...The increasing demand for flexible displays and wearable electronics has driven extensive efforts to develop stretchable organic lightemitting diodes(OLEDs).A critical challenge in this field is the creation of emissive layers that combine high efficiency with mechanical robustness.Thermally activated delayed fluorescence(TADF)materials have attracted significant attention as third-generation emitters capable of achieving 100%internal quantum efficiency;however,their application in stretchable OLEDs has been limited.In this study,we propose an elastomer doping strategy.Polyurethane(PU)is incorporated into TADF polymers to improve their mechanical flexibility while maintaining a high luminescent efficiency.The resulting composite films exhibited excellent TADF characteristics and remarkable stretchability(75%).OLEDs fabricated from these materials achieved a maximum external quantum efficiency(EQE)of 14.26%and a peak luminance of 73570 cd·m^(-2),with the PUdoped devices showing a significantly suppressed efficiency roll-off.Additionally,a fully stretchable OLED architecture was designed and operated under tensile strain to maintain stable electroluminescent performance.These results demonstrate that elastomer doping is an effective strategy for balancing the mechanical compliance with optoelectronic performance,offering a promising pathway for the development of high-performance stretchable OLEDs for flexible electronics.展开更多
Photoswitchable fluorescent polymeric nanoparticles were widely concerned because of their excellent features including the flexible design,easy preparation and functionalization,and thus exhibited great application p...Photoswitchable fluorescent polymeric nanoparticles were widely concerned because of their excellent features including the flexible design,easy preparation and functionalization,and thus exhibited great application potential in information encryption,anti-counterfeiting,but remained challenging in improving the security.Herein,we described a self-erased time-resolved information encryption via using photoswitchable dual-color fluorescent polymeric nanoparticles(PDFPNs)containing two fluorescence dyes(blue and red)and photochromic spiroxazine derivatives.In view of the different thermo-induced isomerization rates of photochromic spiroxazine derivatives in different flexible substrates,the decoloration rate of PDFPNs can be programmatically tuned by regulating ratio between rigid polymer and flexible polymer.Therefore,after ultraviolet light(UV)irradiation,correct information could only be recognized in preestablished time during the self-erased process.Our results indicated that PDFPNs exhibited fast photo-responsibility(2 min),high fluorescence contrast,well-pleasing photo-reversibility(>20 times),and programmable thermo-responsiveness(24 s-6 h).We thus demonstrated their application in the selferased time-resolved information encryption and anti-counterfeiting with high security.展开更多
Very recently,we have proposed that all culprit molecules of arteriosclerosis mightpossess a common and measurable inherent property,namely,a coaggregating ten-dency.Extensive kinetic measurements of these tendencies ...Very recently,we have proposed that all culprit molecules of arteriosclerosis mightpossess a common and measurable inherent property,namely,a coaggregating ten-dency.Extensive kinetic measurements of these tendencies in terms of △CAgC valueshave led to a new and surprising observation,i.e.,the cholesteryl ester with a longsaturated 18-carbon chain(CE-18)actually has a much smaller tendency towardcoaggregation than that of the ester with a“short”8-carbon chain(CE-8).Thisstate of affairs has been coined as the“chain-foldability effect”,i.e.,the 18-carbon展开更多
基金Project supported by the National Natural Science Foundation of China (20505020) the Natural Science Foundation ofGuangdong Province (06300086) +2 种基金 China Postdoctoral Science Foundation (20060390202) Scientific Research Fund ofHunan Provincial Education Department (05C508) Skeleton Youth Faculty Programof Hunan Higher Educational School
文摘The assembling of a coating of time-resolved fluorescent chelator BSPDA （ abbreviated for 4, 7-bis （ sulfhydrylphenyl）-1, 10-phenanthroline-2, 9-dicarboxylic acid） onto a nano-gold layer was demonstrated. First, BSPDA was synthesized by simple procedures, and then an approach was developed to immobilize BSPDA onto the nano-gold layer deposited on a silane modified glass substrate, whereby europium ion （Ⅲ, Eu^3＋ ） was captured and released owing to the interactive process of complexation and dissociation between BSPDA functionalized coating and Eu^3＋ solution. The fluorescence spectra and related lifetimes were determined. Also, the BSPDA functionalized coating＇s specific complexation with Eu^3＋ on the BSPDA assembly layer and the nonspecific adsorption of Eu^3＋ on the nano-gold layer were compared. These results allowed a selective complexation of Eu^3＋ by assembling a BSPDA chelating layer on the nano-gold layer; thus, a tunable time-resolved fluorescent layer was covalently attached, The results of the nanoparticle assembling and probing （or labeling） processes to specific bio-systems were very interesting and had significant implications to time-resolved-fluorescence-based detection on biosensor surfaces such as DNA chip and to arrayed light display devices.
基金Supported by National Key R&D Program for the Prevention and Control of Major Exotic Animal Diseases(2022YFD1800500)National Mutton Sheep Industrial Technology System(CARS39)+2 种基金Key Research and Development Program of Shandong Province(Major Science and Technology Innovation Project)(2021CXGC011306)Scientific Research Project of General Administration of Customs(2024HK033)Scientific Research Project of Jinan Customs(2023JK005).
文摘[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PPR.[Methods]Soluble N protein and NH fusion protein were successfully obtained in an Escherichia coli expression system by optimizing E.coli codon and expression conditions.Furthermore,based on purified soluble N protein and NH fusion protein,a double-antigen sandwich time-resolved fluorescence immunoassay method for detection of peste des petits ruminants virus(PPRV)was established.[Results]The method has high sensitivity and specificity and can specifically detect the antibody against PPRV in sheep serum,and it has no cross reaction with other related diseases.The method was used to detect 292 clinical samples,and compared with French IDVET competition ELISA kit.The coincidence rates of positive samples and negative samples from the two kinds of test kits were 92.47%and 97.26%,respectively,and the overall coincidence rate was 94.86%.The intra-group and inter-group coefficients of variation in the repeatability test were less than 10%.[Conclusions]Compared with the traditional ELISA method,the double-antigen sandwich time-resolved fluorescence immunoassay for detection of PPRV has equivalent sensitivity and specificity,and simple and rapid operation,and thus high application and popularization value.
文摘Timefluorescence decays were first performed on hematoporphyrin derivative laser No.3 in buffer at different micelles concentration.The flu.orescence lifetime constants,measured with a time-correlated single photon counting system;could be fitted by two or three exponential components with different relative peak amplitudes depending on the observation wavelength^thus indicating the presence of different emitting molecular species and/or of molecular species in different aggregational states.
文摘Using a picosecond excitation source and a time-resolved streak camera detection system,the time-resolved fluorescence spectra of purified C_(60) have been recorded from toluene solution at room temperature.A fluorescence peak at about 730nm was detected and its lifetime was directly determined to be 1.1±0.1 ns.Two fluorescence peaks obtained from the mixture of C_(60) and C_(70) at 676 and 696nm were mainly related to C_(70) content.
基金supported by National Key R&D Program of China(2022YFF0710000)National Natural Science Foundation of China(22461142140)Shanghai Jiao Tong University 2030 Initiative。
文摘Accurate quantification of interleukin-6(IL6),a biomarker central to sepsis and cytokine release syndrome,is essential for assessing disease severity.Here,we present a high-performance lateral flow assay(LFA)that leverages a novel conjugate:europium nanoparticles(EuNPs)labels linked via tetrahedral DNA frameworks(TDFs).The TDF precisely controls antibody orientation,minimizes nonspecific binding,and improves conjugate stability.Combined with the strong,time-resolved fluorescence of EuNPs,this design achieves a broad dynamic range and preserves linearity at high analyte concentrations.The platform quantitatively detects IL6 from 0 to 5000 pg/mL within 10 min,showing excellent agreement with reference methods.This DNA-nanostructureenhanced approach provides a robust and portable point-of-care testing strategy for critical clinical decisionmaking.
基金supported by the National Natural Science Foundation of China (NSFC) (No. U1736101)the Youth Innovation Promotion Association CAS (No. 2017155)。
文摘An enhancement method of rapid lifetime determination is proposed for time-resolved fluorescence imaging to discriminate substances with approximate fluorescence lifetime in forensic examination. In the method, an image-exclusive-OR treatment with filter threshold adaptively chosen is presented to extract the region of interest from dual-gated fluorescence intensity images, and then the fluorescence lifetime image is reconstructed based on the rapid lifetime determination algorithm. Furthermore, a maximum and minimum threshold filtering is developed to automatically realize visualization enhancement of the lifetime image. In proof experiments, compared with traditional fluorescence intensity imaging and rapid lifetime determination method, the proposed method automatically distinguishes altered and obliterated documents written by two brands of highlighters with the same color and close fluorescence lifetime.
基金supported in part by the grants from the National Natural Science Foundation of China(grant number 32272400)the Science and Technology Planning Project of Guangzhou City(grant number 202206010146)the National Key Research and Development Program of China(2023YFF1105002).
文摘In recent years,phosphodiesterase type 5 enzyme inhibitors,including tadalafil,sildenafil,and their analogues have emerged as illegal ingredients in dietary supplements.It can cause serious clinical side effects in consumers,resulting in diverse symptoms.To rapidly screen illegal drugs in dietary supplements,a dual-labeled probe time-resolved fluorescence immunochromatography assay(DL-TRFICA)based on two broad-spectrum antibodies labeled with time-resolved fluorescent microspheres was established for the simultaneous qualitative and quantitative detection of nine sildenafil-like and three tadalafil-like adulterants.The half-maximal inhibitory concentration(IC50)of sildenafil-like and tadalafil-like adulterants were 2.49-5.88 and 2.15-2.78 ng mL^(-1),the limit of detection were 0.08-1.66 and 0.59-1.85 ng mL^(-1),and the visualization value(cut-off value)were 31.25-250 and 50-62.50 ng mL^(-1),respectively.Notably,the potential practical applications of DL-TRFICA evaluated by conducting a quantitative evaluation of sildenafil and tadalafil adulterants in dietary supplement samples(herbal spirit drinks,healthcare oral liquids,and herbal medicine pills)demonstrated a recovery rate ranging from 80.0%to 125.8%with a coefficient of variation of less than 15%,which concluded that the DL-TRFICA approach could be used in real sample detection.
基金funded by the National Natural Science Foundation of China(32072920 and 32373067)the National Key Research and Development Programs of China(2023YFD1301001)+1 种基金the HZAU-AGIS Cooperation Fund(SZYJY2022024)the Fundamental Research Funds for the Central Universities(2662022DKPY007).
文摘Fumonisins(FBs)are mycotoxins primarily synthesized by Fusarium moniliformis.Among these,FB1 exhibits not only high toxicity towards humans and animals but also carcinogenic properties.The global prevalence of FB1 contamination in cereals and related products,particularly maize,is alarmingly significant.Consequently,the accurate determination of FB1 levels in cereals holds immense importance.In this study,highly sensitive monoclonal antibodies specifically targeting FB1 were prepared and utilized for the establishment of a time-resolved fluorescence immunochromatographic assay(TRFIC)to detect FB1.The parameters of antibody labeling with time-resolved fluorescent microspheres were optimized.The detection time was significantly reduced to 6 min.The limits of detection(LOD)for corn,rice,and feed were determined as 0.496-0.844μg/kg,and the quantification(LOQ)was 0.788-1.322μg/kg.In addition,an indirect competitive enzyme-linked immunosorbent assay(ic-ELISA)was successfully developed.Under optimized conditions,the half inhibitory concentration(IC50)value for FB1 was determined as 2.137μg/L.A strong correlation between the results obtained from these two methods and HPLC-MS/MS analysis was observed in the same samples tested.In conclusion,both immunological methods developed in this work are highly suitable for rapid FB1 detection in real field samples.
文摘Objective To investigate the prevalence and clinical significance of the centromere protein-F-like(CENP-F-like)immunofluorescence staining pattern in a large patient cohort and through literature review.Methods We retrospectively analyzed antinuclear antibody(ANA)immunofluorescence assay results from 191274 patients at West China Hospital of Sichuan University between March 2018 and November 2020.Specific immunological markers were tested in sera with CENP-F-like patterns.Additionally,a narrative review of seven relevant studies was performed for comparison.Results In Southwest China,ANA positivity was found in 32.09%of patients,with the CENP-F-like pattern detected in 0.015%of all cases and 0.05%of ANA-positive individuals.The CENP-F-like pattern appeared predominantly at titers≥1∶320,most often in isolation(68.97%),but also mixed with cytoplasmic speckled patterns.Patients with cancers accounted for the highest proportion(31.03%),including solid tumors and hematologic malignancies.Metastasis was observed in patients with solid tumors,while graft-versus-host disease(GVHD)occurred in those with hematologic malignancies post-transplantation.Autoimmune diseases(AIDs)were diagnosed in 20.69%of cases,all showing disease-specific autoantibodies.These findings were broadly consistent with previous reports and suggest a possible association between the CENP-F-like pattern and malignancies.Conclusion The CENP-F-like pattern is rare in ANA tests but may be associated with clinically important conditions,particularly cancers and AIDs.The occurrence of metastasis and GVHD in patients with this pattern highlights its potential clinical relevance,and concurrent autoantibodies may assist in diagnosing AIDs.
基金support from the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB0520000)the National Key R&D Program of China(No.2022YFA1203200)+3 种基金National Natural Science Foundation of China(No.52273170)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(No.2021030)Postdoctoral Fellowship Program of CPSF(No.GZC20232735)BMS Junior Fellow of Beijing National Laboratory For molecular Science(No.2023BMS20111).
文摘Cyclo[n]Thiophenes(CnTs)are a distinctive class of π-conjugated macrocyclic molecules that have attracted growing attention owing to their structural aesthetics and organic electronic characteristics.However,the development of CnTs has been largely impeded by inefficient synthetic route.In this work,we employ a bridge strategy using bipyridine as bridge to link two quaterthiophene units resulting in Φ-shaped bicyclosystem.This strain-retaining approach improves the synthesis efficiency of the macrocycles.Two new macrocyclic molecules,(4T-2hexyl-2Me)_(2)-DPBP and(4T-2hexyl)_(2)-DPBP,were successfully synthesized in total yield 17%and 16%,respectively.Single-crystal structure of(4T-2hexyl-2Me)_(2)-DPBP reveals that the bipyridine bridge is orthogonally strapped by two quaterthiophene units.Notably,both compounds exhibit aggregation-induced emission enhancement(AIEE)behavior-an unprecedented feature among CnT-based macrocycles.Theoretical calculations reveal that this AIE phenomenon originates from the restriction of intramolecular motion(RIM)in the aggregated state,which suppresses the non-radiative decay channels.These results demonstrate a generalized strategy for the synthesis of functionalπ-conjugated macrocyclic molecules based fluorescent materials.
文摘Over the past ten years,numerous papers have been published on the use of indocyanine green(ICG)fluorescence in liver surgery for hepatocellular carcinoma(HCC).There are many different applications.The first involves targeting superficial tumors in patients with macronodular cirrhosis and an irregular liver surface.In a minimally invasive setting,the lack of tactile feedback on the hepatic surface makes detecting subcapsular HCC with ultrasound alone challenging.ICG fusion images can mimic the tactile feedback of the hand and act as an ultrasound booster.ICG fluorescence can be used to evaluate tumor residues after minimally invasive thermal ablation.ICG fluorescence imaging can also be used to identify the grade of HCC early on and evaluate the microinvasive component.
文摘Based on 4'-(1H-tetrazol-5-yl)-[1,1'-biphenyl]-2,4,6-tricarboxylic acid(H_(4)bta)ligand,zinc metal-organic framework(Zn-MOF):{[Zn_(2)(bta)(bpy)_(2)(H_(2)O)]·1.5H_(2)O}_(n)(bpy=2,2'-bipyridine)was designed and synthesized by hydrothermal method.Its structure was characterized by elemental analysis,IR spectra,X-ray single crystal diffraction,etc.The asymmetric unit of Zn-MOF contains two crystallographically independent Zn^(2+)ions.Through the connection of Zn^(2+)ions via H_(4)bta,a 1D double-layer network structure is formed.Adjacent double-layer networks further form a 2D supramolecular network through hydrogen bonding.Notably,Zn-MOF exhibited excellent fluorescence properties and could efficiently and sensitively detect various water pollutants:4-nitrophenol(4-NP),Cu^(2+),and pyrimethanil(Pth).Additionally,the mechanism of fluorescence sensing was investigated.CCDC:2432230.
基金supported by the National Natural Science Foundation of China(Nos.82372116,U23A2097)Guangdong Basic and Applied Basic Research Foundation(No.2022A1515010620)+2 种基金Shenzhen Medical Research Fund(Nos.B2302047,A2302047)Shenzhen Science and Technology Program(No.JCYJ20220818095806014)Research Team Cultivation Program of Shenzhen University(No.2023QNT019).
文摘The level of glutathione(GSH)is significantly associated with numerous pathological processes,thus,real-time detection of the GSH level is of significance for early diagnosis of GSH-related diseases.Herein,we developed in vivo second near-infrared(NIR-II)window fluorescence(FL)and ratiometric photoacoustic(RPA)dual-modality imaging of GSH using a GSH-activatable probe(LET-14).LET-14 was synthesized based on a rhodamine hybrid xanthene skeleton with a FL shielding 2,4-dinitrobenzene sulfonyl group that can be specifically cleaved by GSH,thus resulting in a markedly bathochromic-shift absorption,a 6.5-fold increase in NIR-II FL intensity(FL_(920))and a 13-fold increase in RPA signal(PA_(880)/PA_(705))in vitro.Intriguingly,LET-14 exhibits good selectivity and sensitivity for NIR-II FL and RPA dual-modality imaging of GSH in 4T1 tumor-bearing mouse model.Our findings develop an in vivo detection tool of GSH,which has great potential in the field of cancer diagnosis.
基金supported by the National Natural Science Foundation of China[Grant NO.42401465 and 42401464]Yunnan Fundamental Research Projects[Grant NO.202501AT070343,202401AU070169 and 202401CF070161]+1 种基金Natural Science Fund of Kunming University of Science and Technology(KKZ3202421125)Yunnan Provincial Talent Project“High-level Talent Training Support Plan”[YNWR-QNBJ-2020-031]。
文摘Accurate phenological information is essential for measuring ecosystem dynamics and carbon uptake.Southwest China is one of the country's largest terrestrial carbon sink regions and plays a crucial role in carbon peaking and neutrality.However,its complex terrain,fragile ecosystem,and variable climate challenge carbon sink stability.Vegetation phenology significantly impacts carbon absorption and release,making accurate phenological data essential for understanding carbon sequestration dynamics.The widespread distribution of evergreen forests and their weak seasonal variation in canopy introduce significant uncertainties in extracting phenology using traditional remote sensing information in this region.These limitations can lead to inaccurate assessments of carbon sink dynamics.Therefore,precise phenology extraction and analysis are vital for improving ecosystem dynamics and the carbon cycle in Southwest China.Firstly,we employed different ways to evaluate the ability of solar-induced chlorophyll fluorescence(SIF)and traditional remote sensing information to extract phenology.Secondly,based on SIF,we analyzed the spatial and temporal changes in the start of the growing season(SOS),the end of the growing season(EOS),and the length of the growing season(LOS)from 2001 to 2020.Finally,we systematically analyzed the response of SOS and EOS to five preseason climatic factors.The results showed that(1)SIF outperformed traditional remote sensing information in extracting phenology.(2)Vegetation phenology exhibited significant spatial heterogeneity.Moreover,SOS,EOS,and LOS showed trends of advancement,delay,and extension both overall and across all vegetation types.(3)Precipitation was the main factor influencing SOS,while surface downward solar radiation and mean temperature were the main factors affecting EOS,and the phenology of different vegetation types showed a great difference in response to preseason climate factors.These findings improve our understanding of vegetation phenology and its dynamics over Southwest China.
基金sponsored by the National Natural Science Foundation of China(Grant number 42250205,42471510)the Open Found of Technology Innovation Center for Intelligent Monitoring and Spatial Regulation of Land Carbon Sinks,MNR(CUG-SRCS-0002)the Open Fund of Hubei Key Laboratory of Regional Ecological Process and Environmental Evolution(REEC-OF-202405).
文摘Accurately assessing the carbon sequestration capacity of forests is crucial for mitigating climate change.Traditional methods for estimating Gross Primary Productivity(GPP)of vegetation involve significant uncertainties.As a novel remote sensing approach,Solar-Induced chlorophyll Fluorescence(SIF)is directly related to photosynthesis and has demonstrated strong correlations with GPP across various ecosystems,climate zones,and spatial scales.Current GPP estimation methods based on SIF include Light Use Efficiency(LUE)models,the SCOPE process models,and the latest mechanistic light response(MLR)models.Future research should focus on improving the mechanistic understanding of SIF-related processes and promoting the integration of multi-source remote sensing data with SIF-based modeling to enhance the accuracy and universality of GPP estimation.
文摘A series of blue and blue‑green Ir(Ⅲ)complexes has been investigated theoretically to explore their electronic structures,photophysical properties,efficiency roll‑off effect,and thermal activation delayed fluorescence(TADF)properties.All calculations were performed using density functional theory(DFT)and time‑dependent density functional theory(TDDFT).Calculations for electronic structures,frontier molecular orbital characteristics(which determine the efficiency roll‑off effect of the complexes),and photophysical properties were conducted using the Gaussian 09 software package.The calculation of spin‑orbit coupling matrix elements<T|HSOC|S>,which determine the TADF properties of the complexes,was performed using the ORCA software package.The calculation results show that the auxiliary ligand tetraphenylimidodiphosphinate(tpip),a strong electron‑withdrawing group,can mitigate the efficiency roll‑off effect of the complex.Furthermore,TADF is observed in one of the designed complexes,(F_(3)Phppy)_(2)Ir(tpip),where F_(3)Phppy=2‑[4‑(2,4,6‑trifluorophenyl)phenyl]pyridine.
文摘Existing methods for tracing water pollution sources typically integrate three-dimensional excitationemission matrix(3D-EEM)fluorescence spectroscopy with similarity-based matching algorithms.However,these approaches exhibit high error rates in borderline cases and necessitate expert manual review,which limits scalability and introduces inconsistencies between algorithmic outputs and expert judgment.To address these limitations,we propose a large vision-language model(VLM)designed as an“expert agent”to automatically refine similarity scores,ensuring alignment with expert decisions and overcoming key application bottlenecks.The model consists of two core components:(1)rule-based similarity calculation module generate initial spectral similarity scores,and(2)pre-trained large vision-language model fine-tuned via supervised learning and reinforcement learning with human feedback(RLHF)to emulate expert assessments.To facilitate training and evaluation,we introduce two expert-annotated datasets,Spec1k and SpecReason,which capture both quantitative corrections and qualitative reasoning patterns,allowing the model to emulate expert decision-making processes.Experimental results demonstrate that our method achieves 81.45%source attribution accuracy,38.24%higher than rule-based and machine learning baselines.Real-world deployment further validates its effectiveness.
基金supported by the National Natural Science Foundation of China(Nos.T2441002,22525506,U24A20137,and U22A6002)Strategic Priority Research Program of CAS(No.XDB0520101)+1 种基金National Key R&D Program of China(No.2023YFB3609000)CAS Project for Young Scientists in Basic Research(No.YSBR-053)。
文摘The increasing demand for flexible displays and wearable electronics has driven extensive efforts to develop stretchable organic lightemitting diodes(OLEDs).A critical challenge in this field is the creation of emissive layers that combine high efficiency with mechanical robustness.Thermally activated delayed fluorescence(TADF)materials have attracted significant attention as third-generation emitters capable of achieving 100%internal quantum efficiency;however,their application in stretchable OLEDs has been limited.In this study,we propose an elastomer doping strategy.Polyurethane(PU)is incorporated into TADF polymers to improve their mechanical flexibility while maintaining a high luminescent efficiency.The resulting composite films exhibited excellent TADF characteristics and remarkable stretchability(75%).OLEDs fabricated from these materials achieved a maximum external quantum efficiency(EQE)of 14.26%and a peak luminance of 73570 cd·m^(-2),with the PUdoped devices showing a significantly suppressed efficiency roll-off.Additionally,a fully stretchable OLED architecture was designed and operated under tensile strain to maintain stable electroluminescent performance.These results demonstrate that elastomer doping is an effective strategy for balancing the mechanical compliance with optoelectronic performance,offering a promising pathway for the development of high-performance stretchable OLEDs for flexible electronics.
基金financially supported by the National Key R&D Program of China(Nos.2023YFB3812400,2023YFB3812403)National Natural Foundation of China(Nos.52273206,52350233)+1 种基金Hunan Provincial Natural Science Foundation(No.2021JJ10029)Huxiang High-level Talent Gathering Project(No.2022RC4039).
文摘Photoswitchable fluorescent polymeric nanoparticles were widely concerned because of their excellent features including the flexible design,easy preparation and functionalization,and thus exhibited great application potential in information encryption,anti-counterfeiting,but remained challenging in improving the security.Herein,we described a self-erased time-resolved information encryption via using photoswitchable dual-color fluorescent polymeric nanoparticles(PDFPNs)containing two fluorescence dyes(blue and red)and photochromic spiroxazine derivatives.In view of the different thermo-induced isomerization rates of photochromic spiroxazine derivatives in different flexible substrates,the decoloration rate of PDFPNs can be programmatically tuned by regulating ratio between rigid polymer and flexible polymer.Therefore,after ultraviolet light(UV)irradiation,correct information could only be recognized in preestablished time during the self-erased process.Our results indicated that PDFPNs exhibited fast photo-responsibility(2 min),high fluorescence contrast,well-pleasing photo-reversibility(>20 times),and programmable thermo-responsiveness(24 s-6 h).We thus demonstrated their application in the selferased time-resolved information encryption and anti-counterfeiting with high security.
基金the National Natural Science Foundation of China Laboratory of Photochemistry and PhotophysicsInstitute of Photographic Chemistry,Chinese Academy of Sciences.
文摘Very recently,we have proposed that all culprit molecules of arteriosclerosis mightpossess a common and measurable inherent property,namely,a coaggregating ten-dency.Extensive kinetic measurements of these tendencies in terms of △CAgC valueshave led to a new and surprising observation,i.e.,the cholesteryl ester with a longsaturated 18-carbon chain(CE-18)actually has a much smaller tendency towardcoaggregation than that of the ester with a“short”8-carbon chain(CE-8).Thisstate of affairs has been coined as the“chain-foldability effect”,i.e.,the 18-carbon
