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The Stability for the Hyperneutral Type Nonlinear Time Varying Control Systems(1) 被引量:2
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作者 Mong Peiyuan Li Yeping 《Wuhan University Journal of Natural Sciences》 CAS 1998年第3期23-28,共6页
We discuss the equivalence problem of hyperneutral type nonlinear time varying control system and linear time varying control system in the theory of stabilization.Making use of the symmetric positive definite soluti... We discuss the equivalence problem of hyperneutral type nonlinear time varying control system and linear time varying control system in the theory of stabilization.Making use of the symmetric positive definite solution of Riccati matrix differential equation which corresponds to the linear time varying control system, we construct the Lyapunov function.Then we provide the sufficent conditions which make that the zero solution of the hyperneutral type nonlinear time varying control systems is uniformly asymptotically stable by means of the Lyapunov equivalence decomposition method, and find the formulaes which can be used to estimate the bounds of the time delays and the nonlinear terms. 展开更多
关键词 hyperneutral type time delay NONLINEAR asymptotically stable
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The effect of polymer type on electric breakdown strength on a nanosecond time scale 被引量:1
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作者 赵亮 苏建仓 +1 位作者 潘亚峰 张喜波 《Chinese Physics B》 SCIE EI CAS CSCD 2012年第3期158-163,共6页
Based on the concepts of fast polarization, effective electric field and electron impact ionization criterion, the effect of polymer type on electric breakdown strength (EBD) on a nanosecond time scale is investigat... Based on the concepts of fast polarization, effective electric field and electron impact ionization criterion, the effect of polymer type on electric breakdown strength (EBD) on a nanosecond time scale is investigated, and a formula that qualitatively characterizes the relation between the electric breakdown strength and the polymer type is derived. According to this formula, it is found that the electric breakdown strength decreases with an increase in the effective relative dielectric constants of the polymers. By calculating the effective relative dielectric constants for different types of polymers, the theoretical relation for the electric breakdown strengths of common polymers is predicted. To verify the prediction, the polymers of PE (polyethylene), PTFE (polytetrafluoroethelene), PMMA (organic glass) and Nylon are tested with a nanosecond-pulse generator. The experimental result shows EBD (PTFE) 〉 EBD (PMMA) 〉 EBD (Nylon) 〉 EBD (PE). This result is consistent with the theoretical prediction. 展开更多
关键词 electric breakdown strength polymer type nanosecond time scale fast polarization phenomenon
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Energy-Momentum Density of Non-Diagonal Bianchi Type Space-Time in General Relativity and Teleparallel Gravity
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作者 Ragab M. Gad Haifaa A. Alharbi 《Journal of Applied Mathematics and Physics》 2021年第9期2355-2375,共21页
We calculated the energy-momentum density of non-diagonal Bianchi type space-time in two different theories of gravity, General relativity (GR) and the theory of Teleparallel gravity (TG). Firstly, by applying Einstei... We calculated the energy-momentum density of non-diagonal Bianchi type space-time in two different theories of gravity, General relativity (GR) and the theory of Teleparallel gravity (TG). Firstly, by applying Einstein, Landau-Lifshitz, Bergmann-Thomson and M<span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;">&#248;</span></span></span>ller prescriptions, using double index complexes in <strong>GR</strong>. Secondly, in the frame work of <strong>TG</strong>, we used the energy momentum complexes of Einstein, Bergmann-Thomson and Landau-Lifshitz. We also study the spacial cases of non-diagonal Bianchi type space-time <strong>BII</strong>, <strong>BVIII</strong> and <strong>BIX</strong>. We obtained the same energy-momentum density components for Einstein and Bergmann-Thomson prescriptions for the above four mentioned space-times that we considered in our work. Also, we found that the energy density component in M<span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;">&#248;</span></span></span>ller prescription is zero for all Bianchi types space-times in GR. Furthermore, we show that if the metric components are functions of time t alone, then the total gravitational energy is identically zero. 展开更多
关键词 Energy-Momentum Densities Double Index Complexes Teleparallel Gravity Bianchi type Space time
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Existence of Periodic Solutions for Neutral-Type Neural Networks with Delays on Time Scales 被引量:1
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作者 Zhenkun Huang Jinxiang Cai 《Journal of Applied Mathematics and Physics》 2013年第4期1-5,共5页
In this paper, we employ a fixed point theorem due to Krasnosel’skii to attain the existence of periodic solutions for neutral-type neural networks with delays on a periodic time scale. Some new sufficient conditions... In this paper, we employ a fixed point theorem due to Krasnosel’skii to attain the existence of periodic solutions for neutral-type neural networks with delays on a periodic time scale. Some new sufficient conditions are established to show that there exists a unique periodic solution by the contraction mapping principle. 展开更多
关键词 Neutral-type NEURAL Networks On time Scales PERIODIC Solution
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Time-delay effects on the dynamics of Linard type equation with fast and slow variables
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作者 Yuanguang Zheng Lijuan Bao 《Theoretical & Applied Mechanics Letters》 CAS 2013年第6期47-50,共4页
Time-delay effects on the dynamics of Li^nard type equation with one fast variable and one slow variable are investigated in the present paper. By using the methods of stability switch and geometric singular perturbat... Time-delay effects on the dynamics of Li^nard type equation with one fast variable and one slow variable are investigated in the present paper. By using the methods of stability switch and geometric singular perturbation, time-delay-induced complex oscillations and bursting are investigated, and in several case studies, the mechanism of the generation of the complex oscillations and bursting is illuminated. Numerical results demonstrate the validity of the theoretical results. 展开更多
关键词 Lienard type equation time-DELAY stability switch singular perturbation
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Effects of Seed Maturity of Turf-Type Tall Fescue on the Seed Vigor and the Optimal Harvesting Time 被引量:3
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作者 XU Rong and HAN Jian-guoInstitute of Grassland Science, China Agricultural University, Beijing 100094 , P.R.China 《Agricultural Sciences in China》 CAS CSCD 2003年第4期454-462,共9页
By assay of accelerated aging germination, germination index, vigor index, seedling length, seedling weight, electric conductivity, dehydrogenase activity, ATP content and acid phosphoesterase activity during seed dev... By assay of accelerated aging germination, germination index, vigor index, seedling length, seedling weight, electric conductivity, dehydrogenase activity, ATP content and acid phosphoesterase activity during seed development of turf-type tall fescue, the seed vigor of tall fescue were studied. Combining with seed moisture content and yield, the optimal harvesting time of tall fescue was determined. The results indicated that the seed vigor increased continuously along with seed maturity, and the higher seed vigor was achieved at 19th day after perk anthesis and maintained continuously until 31st day after peak anthesis. At 25th day after peak anthesis, the highest yield of 3 533 kg ha-1 and the good quality of seeds of tall fescue were harvested with 32.19% moisture content. From 22nd to 31st day after peak anthesis, the seed yield of 3 300 kg ha-1 and the good quality seeds of tall fescue were harvested with 40 - 12. 43% moisture content, and the span was the optimal harvesting time. 展开更多
关键词 Turf-type tall fescue Seed vigor Optimal harvesting time
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Integral Inequality of Hardy-Type on Time Scales
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作者 Emmanuella Ehui Aribike Yisa Oluwatoyin Anthonio +1 位作者 Kamilu Rauf Michael Oyelami Ajisope 《Advances in Pure Mathematics》 2019年第6期534-543,共10页
Inequalities are essential in the study of Mathematics and are useful tools in the theory of analysis. They have been playing a critical role in the study of the existence and uniqueness properties of solutions of ini... Inequalities are essential in the study of Mathematics and are useful tools in the theory of analysis. They have been playing a critical role in the study of the existence and uniqueness properties of solutions of initial and boundary value problems for differential equations as well as difference equations with their bounds. In this paper, we obtain new integral inequalities mainly by using some known inequalities. Various generalizations of Hardy's inequality are special cases of the results therein. 展开更多
关键词 time Scale Measurable CONVEX FUNCTIONS Hardy-type INEQUALITIES
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Bianchi Type-IX Anisotropic Dark Energy Cosmological Models with Time Dependent Deceleration Parameter
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作者 H. R. Ghate Atish S. Sontakke Yogendra D. Patil 《International Journal of Astronomy and Astrophysics》 2015年第4期302-323,共22页
Bianchi type-IX cosmological models with variable equation of state (EoS) parameter have been investigated in general relativity when universe is filled with dark energy. The field equations have been solved by consid... Bianchi type-IX cosmological models with variable equation of state (EoS) parameter have been investigated in general relativity when universe is filled with dark energy. The field equations have been solved by considering (i) q=B (variable);(ii) , where k and m are constants;(iii) , where k is constant and R is average scale factor;(iv) which gives . This renders early decelerating and late time accelerating cosmological models. The physical and geometrical properties of the models are also discussed. 展开更多
关键词 DARK Energy DECELERATION Parameter Bianchi type-IX Space time
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Numerical Solutions for the Time-Dependent Emden-Fowler-Type Equations by B-Spline Method
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作者 Mohamed El-Gamel W. El-bashbashy Atallah El-Shenawy 《Applied Mathematics》 2014年第4期593-600,共8页
A numerical method based on B-spline is developed to solve the time-dependent Emden-Fow- ler-type equations. We also present a reliable new algorithm based on B-spline to overcome the difficulty of the singular point ... A numerical method based on B-spline is developed to solve the time-dependent Emden-Fow- ler-type equations. We also present a reliable new algorithm based on B-spline to overcome the difficulty of the singular point at x = 0. The error analysis of the method is described. Numerical results are given to illustrate the efficiency of the proposed method. 展开更多
关键词 B-SPLINE time-DEPENDENT Numerical Solutions EMDEN-FOWLER type
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Global Existence and Large Time Asymptotic Behavior of Strong Solution to the Cauchy Problem of 2D Density-Dependent Boussinesq Equations of Korteweg Type
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作者 Qi Zhang 《Advances in Pure Mathematics》 2021年第4期346-368,共23页
In this paper, we study the Cauchy problem of the density-dependent Boussinesq equations of Korteweg type on the whole space with a vacuum. It is proved that there exists a unique strong solution for the two-dimension... In this paper, we study the Cauchy problem of the density-dependent Boussinesq equations of Korteweg type on the whole space with a vacuum. It is proved that there exists a unique strong solution for the two-dimensional Cauchy problem established that the initial density and the initial temperature decay not extremely slow. Particularly, it is allowed to be arbitrarily large for the initial data and vacuum states for the initial density, even including the compact support. Moreover, when the density depends on the Korteweg term with the viscosity coefficient and capillary coefficient, we obtain a consistent priority estimate by the energy method, and extend the local strong solutions to the global strong solutions. Finally, when the pressure and external force are not affected, we deform the fluid models of Korteweg type, we can obtain the large time decay rates of the gradients of velocity, temperature and pressure. 展开更多
关键词 Incompressible Boussinesq Equation Korteweg type Global Strong Solutions Large time Behavior Vacuum
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基于时滞补偿的DK-2机车制动滑模自抗扰控制
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作者 肖友刚 胡紫欣 +1 位作者 李蔚 袁玄成 《铁道科学与工程学报》 北大核心 2026年第1期275-288,共14页
为解决机车空气制动系统时滞与扰动导致制动控制性能下降的问题,以DK-2型机车制动机为研究对象,在分析制动机压力控制原理的基础上,设计一种带时滞与扰动补偿的滑模自抗扰复合控制器(sliding mode active disturbance rejection control... 为解决机车空气制动系统时滞与扰动导致制动控制性能下降的问题,以DK-2型机车制动机为研究对象,在分析制动机压力控制原理的基础上,设计一种带时滞与扰动补偿的滑模自抗扰复合控制器(sliding mode active disturbance rejection controller with time-delay and disturbance compensation,SMADRCWC)。首先,采用AMESim软件建立其气动系统数值仿真模型,探明时滞对DK-2型制动机的影响程度。在此基础上,设计时滞因子能自适应调整的输出预估跟踪微分器(adaptive output predictive tracking differentiator,AOPTD),在系统动态变化阶段增大以充分补偿时滞,在稳态时减小以避免超调;为了补偿气路泄漏、模型不确定性及外部干扰等总扰动,设计扩张状态观测器(extended state observer,ESO)对其进行实时估计并补偿,将AOPTD、ESO与滑模控制(sliding mode control,SMC)相结合,综合形成具有时滞补偿和扰动抑制功能的闭环控制器SMADRCWC。仿真结果表明:SMADRCWC能够有效提升列车管和制动缸的压力跟踪精度,同时减少电磁阀的频繁动作,提高系统运行的稳定性;在1000 ms时滞工况下,相比PID和滑模控制,该策略能够显著缩短压力调节时间,并有效补偿因时滞导致的动态误差,避免了超调现象的发生;在100~1000 ms时滞范围及复合扰动作用下,SMADRCWC控制下的DK-2型制动机运行平稳无超调,且压力控制误差在±5 kPa范围内,体现出了很强的鲁棒性。所设计的SMADRCWC控制器为不确定时滞和复杂扰动环境的机车空气制动系统控制提供了一种新的解决方法。 展开更多
关键词 DK-2制动机 时滞补偿 输出预估跟踪微分器 扩张状态观测器 滑模自抗扰控制
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基于事件过程的珠江流域极端小时降水变化特征
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作者 宋晓猛 韦金江 张建云 《水科学进展》 北大核心 2026年第2期220-234,共15页
全球变暖背景下极端降水事件频发,对生态环境和人类社会构成严峻挑战,深入理解其变化特征对防洪减灾具有重要意义。本文利用多源数据融合的逐小时降水数据集,基于事件过程的极端小时降水(Event-based Extreme Hourly Precipitation,EEHP... 全球变暖背景下极端降水事件频发,对生态环境和人类社会构成严峻挑战,深入理解其变化特征对防洪减灾具有重要意义。本文利用多源数据融合的逐小时降水数据集,基于事件过程的极端小时降水(Event-based Extreme Hourly Precipitation,EEHP)分析框架,探讨珠江流域2000—2023年EEHP的时空变化特征,重点分析主导时间分布模式、历时-强度组合模式及其空间分布格局。结果表明:①EEHP总频次呈上升趋势,然而平均降水量和持续时间普遍呈下降趋势,年内高发期集中于5—6月,且日内降水主峰值多发于上午07:00—08:00和下午16:002个时段。②EEHP的各项指标具有相似的空间分布格局,总体为“西低东高”的分布特征。③前峰型时间分布模式为珠江流域EEHP的主导类型(占比87.4%),主要分布于流域西部的云贵-广西、东部的广东-福建及两广沿海地区,在春夏季最为活跃。④长历时-低强度EEHP是珠江流域事件占比最高的类型,低强度事件的空间分布呈“西北低、东南高”的格局,高强度事件的空间分布则相对均匀;不同组合类型的EEHP事件平均强度与平均历时呈现明显的逆向关系,平均历时随着平均强度增加而显著减小。 展开更多
关键词 极端小时降水 时间分布模式 季节性主导 历时-强度类型
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套袋对‘红阳’猕猴桃果实品质的影响
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作者 陈厚锡 姜连 +4 位作者 李良良 李苇洁 罗充 高洁静 张青青 《分子植物育种》 北大核心 2026年第2期525-529,共5页
为提升猕猴桃套袋技术,以‘红阳’猕猴桃为试材,探究3种不同质地果袋及3个不同套袋时间处理对猕猴桃果实品质的影响。结果显示,套袋可改善‘红阳’猕猴桃果实外观,降低其果实内含物;增添果袋层数和提早套袋时间均能降低果实风斑率和病... 为提升猕猴桃套袋技术,以‘红阳’猕猴桃为试材,探究3种不同质地果袋及3个不同套袋时间处理对猕猴桃果实品质的影响。结果显示,套袋可改善‘红阳’猕猴桃果实外观,降低其果实内含物;增添果袋层数和提早套袋时间均能降低果实风斑率和病虫危害率,且差异显著;基于果实8个主要指标隶属函数值的综合评价发现,花后20 d套黄色单层袋的果实品质得分最高。本研究可为‘红阳’猕猴桃套袋的果袋择取及其施行时间提供理论支撑。 展开更多
关键词 ‘红阳’猕猴桃 套袋时间 袋型 果实品质
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压力型抗浮锚杆承载性能现场试验研究
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作者 白晓宇 郑海龙 +2 位作者 孙淦 吕承禄 闫楠 《河北工程大学学报(自然科学版)》 2026年第1期9-16,共8页
为深入研究压力型预应力混凝土用螺纹钢筋(PSB)抗浮锚杆的承载性能和位移规律,以青岛某实际抗浮工程为例,针对3根压力型PSB抗浮锚杆开展现场试验研究。通过实时监测锚筋与灌浆体的位移变化,系统分析了荷载-位移演化规律,揭示了PSB抗浮... 为深入研究压力型预应力混凝土用螺纹钢筋(PSB)抗浮锚杆的承载性能和位移规律,以青岛某实际抗浮工程为例,针对3根压力型PSB抗浮锚杆开展现场试验研究。通过实时监测锚筋与灌浆体的位移变化,系统分析了荷载-位移演化规律,揭示了PSB抗浮锚杆的黏结应力分布特性。试验结果表明,锚筋与灌浆体位移随荷载增长呈线性增加趋势,受荷载水平影响显著;当荷载达到最大荷载的60%时,位移曲线出现骤升,进入加速增长阶段;灌浆体位移曲线整体呈线性增长,未出现突变点或双折线特征,锚筋与灌浆体的相对位移随着荷载增加而增大。该试验条件下,压力型PSB抗浮锚杆锚筋-灌浆体界面展现出的平均黏结应力为0.73 MPa,而灌浆体-岩土体界面的平均黏结应力则相对较低,为0.104 MPa。灌浆体-岩土体界面黏结应力与锚固长度和锚孔直径有关,锚固长度和锚孔直径过大反而会降低锚筋-灌浆体界面的黏结应力。 展开更多
关键词 压力型PSB抗浮锚杆 时间-位移曲线 黏结应力 现场试验
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猪IL-4、IL-6和IL-10 SYBR Green Ⅰ real-time PCR检测方法的建立及应用 被引量:11
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作者 施开创 李焕荣 +2 位作者 杨汉春 郭鑫 盖新娜 《中国预防兽医学报》 CAS CSCD 北大核心 2010年第2期102-107,共6页
为探讨PRRSV感染后机体的体液免疫应答、从分子水平深入研究PRRSV的免疫机制,本研究针对Th2型细胞因子IL-4、IL-6、IL-10以及看家基因β-actin的基因序列分别设计一对特异性引物,构建含有各自引物扩增序列的重组质粒作为阳性标准品,建... 为探讨PRRSV感染后机体的体液免疫应答、从分子水平深入研究PRRSV的免疫机制,本研究针对Th2型细胞因子IL-4、IL-6、IL-10以及看家基因β-actin的基因序列分别设计一对特异性引物,构建含有各自引物扩增序列的重组质粒作为阳性标准品,建立了检测IL-4、IL-6、IL-10及β-actin的SYBR Green Ⅰ real-time PCR方法。该方法线性关系好,各种细胞因子及β-actin标准曲线的相关系数均达到0.997以上;敏感性高,初始模板的检出下限均达到1×101copies/μL;特异性强,扩增产物形成单一的特异性熔解峰;重复性好,组内与组间的变异系数均小于3%。应用所建立的方法对猪繁殖与呼吸综合征病毒(PRRSV)感染仔猪外周血单个核细胞(PBMC)中IL-4、IL-6和IL-10mRNA的表达水平进行了检测。结果表明,本研究建立的real-timePCR检测方法灵敏度高、特异性强、重复性好,可以用于猪Th2型细胞因子的检测及定量分析。 展开更多
关键词 TH2型细胞因子 荧光定量PCR 检测方法
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绵羊IFN-γ和IL-2基因SYBR GreenⅠreal-time PCR检测方法的建立及应用 被引量:5
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作者 张冰冰 赵魁 +6 位作者 宋德光 臧德跃 王栋 贺文琦 陈克研 王改丽 高丰 《中国兽医科学》 CAS CSCD 北大核心 2010年第11期1146-1150,共5页
为探讨传染性脓疱病毒感染后机体的细胞免疫应答,从分子水平对传染性脓疱病毒的免疫机制进行了深入探讨。首先针对Th1型细胞因子IFN-γ、IL-2以及管家基因GAPDH的mRNA序列分别设计了1对特异性引物,扩增相应的基因片段并构建含有相应基... 为探讨传染性脓疱病毒感染后机体的细胞免疫应答,从分子水平对传染性脓疱病毒的免疫机制进行了深入探讨。首先针对Th1型细胞因子IFN-γ、IL-2以及管家基因GAPDH的mRNA序列分别设计了1对特异性引物,扩增相应的基因片段并构建含有相应基因序列的重组质粒,以所构建的重组质粒作为阳性标准品,建立了用于IFN-γ、IL-2及GAPDH检测的SYBR GreenⅠreal-time PCR方法。该方法线性关系良好,IFN-γ、IL-2和GAPDH标准曲线的相关系数均达0.99以上;敏感性高,初始模板的检出下限均达1×103copies/μL;特异性强,扩增产物形成单一的特异性融解峰;重复性好,组内与组间的变异系数均小于3%。应用该方法对传染性脓疱病毒感染羔羊外周血白细胞中的IFN-γ、IL-2 mRNA的表达水平进行了检测。结果表明,建立的real-time PCR检测方法具有灵敏度高、特异性强、重复性好等优点,可用于绵羊Th1型细胞因子的检测及定量分析。 展开更多
关键词 羔羊 TH1型细胞因子 荧光定量聚合酶链反应 检测方法
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Type-2模糊系统在黄金价格预测中的应用 被引量:3
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作者 钱冰冰 《佳木斯大学学报(自然科学版)》 CAS 2007年第3期397-399,共3页
选择了一个模糊时间序列的模型来确定模糊系统初始参数,同时运用了Type-2模糊系统和Type-1模糊系统进行了训练及预测,比较了他们的训练逼近效果,以及预测效果,给出了仿真.
关键词 模糊时间序列 type-2模糊系统 模糊系统训练 预测
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猪链球菌2型的Real-time PCR定量检测研究 被引量:6
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作者 蒋鲁岩 蔡潭溪 陈国强 《中国人兽共患病学报》 CAS CSCD 北大核心 2006年第9期843-845,850,共4页
目的 建立了基于TaqMan探针的Real-time PCR方法,实时、定量检测猪链球菌2型。针对GenBank公布的猪链球菌2型的csp2J基因序列设计一对引物和TaqMan探针,建立了Real-time PCR检测方法,制作标准曲线,定量检测猪链球菌2型。通过对6种... 目的 建立了基于TaqMan探针的Real-time PCR方法,实时、定量检测猪链球菌2型。针对GenBank公布的猪链球菌2型的csp2J基因序列设计一对引物和TaqMan探针,建立了Real-time PCR检测方法,制作标准曲线,定量检测猪链球菌2型。通过对6种细菌(9株菌株)的DNA进行扩增。结果所有4株猪链球菌2型均可产生扩增曲线,其他5株非猪链球菌2型均不产生扩增曲线。细菌纯培养物的检测敏感度为6~8CFU/PCR反应体系,相关系数均为0.99(r^2=0.99),整个试验可在1.5h内完成。建立的方法可用于猪链球菌2型的快速、定量检测。 展开更多
关键词 TAQMAN探针 REAL-time PCR 猪链球菌csp2J基因 定量检测
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猪IL-2、IL-12p40和IFN-γ SYBR Green Ⅰ real-time PCR检测方法的建立 被引量:2
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作者 施开创 莫胜兰 +4 位作者 许瑞胜 李向涛 陈宏备 郑敏 刘棋 《中国畜牧兽医》 CAS 北大核心 2010年第7期74-79,共6页
针对猪Th1型细胞因子IL-2、IL-12p40、IFN-γ以及管家基因β-actin的基因序列分别设计一对特异性引物,构建含有各自引物扩增序列的重组质粒作为阳性标准品,建立了检测IL-2、IL-12p40、IFN-γ及β-actin的SYBR Green Ⅰ real-ti mePCR方... 针对猪Th1型细胞因子IL-2、IL-12p40、IFN-γ以及管家基因β-actin的基因序列分别设计一对特异性引物,构建含有各自引物扩增序列的重组质粒作为阳性标准品,建立了检测IL-2、IL-12p40、IFN-γ及β-actin的SYBR Green Ⅰ real-ti mePCR方法。该方法线性关系好,标准曲线的相关系数均达到0.997以上;敏感性高,初始模板的检出下限均达到100拷贝/μL;特异性强,扩增产物形成单一的特异性熔解峰;重复性好,组内与组间的变异系数均小于3.5%。应用所建立的方法对猪繁殖与呼吸综合征病毒感染仔猪外周血单个核细胞中IL-2、IL-12p40和IFN-γ mRNA的表达水平进行了检测。结果表明,所建立的real-time PCR检测方法灵敏度高、特异性强、重复性好。 展开更多
关键词 TH1型细胞因子 荧光定量PCR SYBR Green
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猪细环病毒1型real-time PCR检测方法的建立 被引量:5
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作者 王俊 单晶晶 +3 位作者 孟凡伟 于红欣 黎阳 周双海 《中国兽医科学》 CAS CSCD 北大核心 2014年第9期927-932,共6页
参考猪细环病毒1型(TTSuV 1)基因非编码区保守序列设计了1对特异性引物,构建含有TTSuV 1基因片段的重组质粒,以系列稀释后的重组质粒作为模板建立了定量检测TTSuV 1的SYBR GreenⅠreal-time PCR方法,并绘制标准曲线,进行特异性、敏感性... 参考猪细环病毒1型(TTSuV 1)基因非编码区保守序列设计了1对特异性引物,构建含有TTSuV 1基因片段的重组质粒,以系列稀释后的重组质粒作为模板建立了定量检测TTSuV 1的SYBR GreenⅠreal-time PCR方法,并绘制标准曲线,进行特异性、敏感性和重复性试验。结果显示,该方法标准曲线的决定系数为0.999 5,表明具有优良的线性关系;最低可精确检测63copies/μL的核酸模板;重复性试验的变异系数小于2%;对TTSuV 2、猪圆环病毒1型、猪圆环病毒2型、伪狂犬病病毒、猪细小病毒、猪繁殖与呼吸综合征病毒及猪瘟病毒等均检测不到荧光信号。对临床样品的检出率高于常规PCR方法。结果表明,建立的real-time PCR检测方法特异性强、灵敏度高、重复性好,可用于TTSuV 1的检测与定量分析。 展开更多
关键词 猪细环病毒1型 实时定量PCR SYBR GreenⅠ 定量检测
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