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The Stability for the Hyperneutral Type Nonlinear Time Varying Control Systems(1) 被引量:2
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作者 Mong Peiyuan Li Yeping 《Wuhan University Journal of Natural Sciences》 CAS 1998年第3期23-28,共6页
We discuss the equivalence problem of hyperneutral type nonlinear time varying control system and linear time varying control system in the theory of stabilization.Making use of the symmetric positive definite soluti... We discuss the equivalence problem of hyperneutral type nonlinear time varying control system and linear time varying control system in the theory of stabilization.Making use of the symmetric positive definite solution of Riccati matrix differential equation which corresponds to the linear time varying control system, we construct the Lyapunov function.Then we provide the sufficent conditions which make that the zero solution of the hyperneutral type nonlinear time varying control systems is uniformly asymptotically stable by means of the Lyapunov equivalence decomposition method, and find the formulaes which can be used to estimate the bounds of the time delays and the nonlinear terms. 展开更多
关键词 hyperneutral type time delay NONLINEAR asymptotically stable
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考虑蓄电池Run-time等效模型的船舶直流应急电网短路电流计算 被引量:4
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作者 王朝红 曹穆 +1 位作者 许嘉沄 雷加智 《武汉大学学报(工学版)》 CAS CSCD 北大核心 2024年第3期348-355,共8页
为计算直流应急电网不同故障类型下的短路电流,便于直流应急电网开关器件的型号以及相应的保护措施的选择,提出了一种考虑蓄电池Run-time等效模型的船舶直流应急电网短路电流计算方法。与传统蓄电池的Thevenin等效模型和PNGV(the partne... 为计算直流应急电网不同故障类型下的短路电流,便于直流应急电网开关器件的型号以及相应的保护措施的选择,提出了一种考虑蓄电池Run-time等效模型的船舶直流应急电网短路电流计算方法。与传统蓄电池的Thevenin等效模型和PNGV(the partnership for a new generation of vehicles)等效模型相比,所提方法考虑了蓄电池容量衰减、温度、循环次数、存储时长、电流倍率、自身产热等多因素的影响,对蓄电池的故障等值电路进行了精确模拟。最后,利用实际的直流应急电网短路电流仿真和实验验证了所提方法的准确性,与Thevenin等效模型和PNGV等效模型相比,不同故障下蓄电池Run-time等效模型短路电流的计算误差更小。 展开更多
关键词 直流应急电网 Run-time等效模型 短路电流 故障等值 故障类型
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The effect of polymer type on electric breakdown strength on a nanosecond time scale 被引量:1
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作者 赵亮 苏建仓 +1 位作者 潘亚峰 张喜波 《Chinese Physics B》 SCIE EI CAS CSCD 2012年第3期158-163,共6页
Based on the concepts of fast polarization, effective electric field and electron impact ionization criterion, the effect of polymer type on electric breakdown strength (EBD) on a nanosecond time scale is investigat... Based on the concepts of fast polarization, effective electric field and electron impact ionization criterion, the effect of polymer type on electric breakdown strength (EBD) on a nanosecond time scale is investigated, and a formula that qualitatively characterizes the relation between the electric breakdown strength and the polymer type is derived. According to this formula, it is found that the electric breakdown strength decreases with an increase in the effective relative dielectric constants of the polymers. By calculating the effective relative dielectric constants for different types of polymers, the theoretical relation for the electric breakdown strengths of common polymers is predicted. To verify the prediction, the polymers of PE (polyethylene), PTFE (polytetrafluoroethelene), PMMA (organic glass) and Nylon are tested with a nanosecond-pulse generator. The experimental result shows EBD (PTFE) 〉 EBD (PMMA) 〉 EBD (Nylon) 〉 EBD (PE). This result is consistent with the theoretical prediction. 展开更多
关键词 electric breakdown strength polymer type nanosecond time scale fast polarization phenomenon
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Energy-Momentum Density of Non-Diagonal Bianchi Type Space-Time in General Relativity and Teleparallel Gravity
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作者 Ragab M. Gad Haifaa A. Alharbi 《Journal of Applied Mathematics and Physics》 2021年第9期2355-2375,共21页
We calculated the energy-momentum density of non-diagonal Bianchi type space-time in two different theories of gravity, General relativity (GR) and the theory of Teleparallel gravity (TG). Firstly, by applying Einstei... We calculated the energy-momentum density of non-diagonal Bianchi type space-time in two different theories of gravity, General relativity (GR) and the theory of Teleparallel gravity (TG). Firstly, by applying Einstein, Landau-Lifshitz, Bergmann-Thomson and M<span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;">&#248;</span></span></span>ller prescriptions, using double index complexes in <strong>GR</strong>. Secondly, in the frame work of <strong>TG</strong>, we used the energy momentum complexes of Einstein, Bergmann-Thomson and Landau-Lifshitz. We also study the spacial cases of non-diagonal Bianchi type space-time <strong>BII</strong>, <strong>BVIII</strong> and <strong>BIX</strong>. We obtained the same energy-momentum density components for Einstein and Bergmann-Thomson prescriptions for the above four mentioned space-times that we considered in our work. Also, we found that the energy density component in M<span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;">&#248;</span></span></span>ller prescription is zero for all Bianchi types space-times in GR. Furthermore, we show that if the metric components are functions of time t alone, then the total gravitational energy is identically zero. 展开更多
关键词 Energy-Momentum Densities Double Index Complexes Teleparallel Gravity Bianchi type Space time
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Existence of Periodic Solutions for Neutral-Type Neural Networks with Delays on Time Scales 被引量:1
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作者 Zhenkun Huang Jinxiang Cai 《Journal of Applied Mathematics and Physics》 2013年第4期1-5,共5页
In this paper, we employ a fixed point theorem due to Krasnosel’skii to attain the existence of periodic solutions for neutral-type neural networks with delays on a periodic time scale. Some new sufficient conditions... In this paper, we employ a fixed point theorem due to Krasnosel’skii to attain the existence of periodic solutions for neutral-type neural networks with delays on a periodic time scale. Some new sufficient conditions are established to show that there exists a unique periodic solution by the contraction mapping principle. 展开更多
关键词 Neutral-type NEURAL Networks On time Scales PERIODIC Solution
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Time-delay effects on the dynamics of Linard type equation with fast and slow variables
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作者 Yuanguang Zheng Lijuan Bao 《Theoretical & Applied Mechanics Letters》 CAS 2013年第6期47-50,共4页
Time-delay effects on the dynamics of Li^nard type equation with one fast variable and one slow variable are investigated in the present paper. By using the methods of stability switch and geometric singular perturbat... Time-delay effects on the dynamics of Li^nard type equation with one fast variable and one slow variable are investigated in the present paper. By using the methods of stability switch and geometric singular perturbation, time-delay-induced complex oscillations and bursting are investigated, and in several case studies, the mechanism of the generation of the complex oscillations and bursting is illuminated. Numerical results demonstrate the validity of the theoretical results. 展开更多
关键词 Lienard type equation time-DELAY stability switch singular perturbation
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Multi-type ant system algorithm for the time dependent vehicle routing problem with time windows 被引量:16
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作者 DENG Ye ZHU Wanhong +1 位作者 LI Hongwei ZHENG Yonghui 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2018年第3期625-638,共14页
The time dependent vehicle routing problem with time windows(TDVRPTW) is considered. A multi-type ant system(MTAS) algorithm hybridized with the ant colony system(ACS)and the max-min ant system(MMAS) algorithm... The time dependent vehicle routing problem with time windows(TDVRPTW) is considered. A multi-type ant system(MTAS) algorithm hybridized with the ant colony system(ACS)and the max-min ant system(MMAS) algorithms is proposed. This combination absorbs the merits of the two algorithms in solutions construction and optimization separately. In order to improve the efficiency of the insertion procedure, a nearest neighbor selection(NNS) mechanism, an insertion local search procedure and a local optimization procedure are specified in detail. And in order to find a balance between good scouting performance and fast convergence rate, an adaptive pheromone updating strategy is proposed in the MTAS. Computational results confirm the MTAS algorithm's good performance with all these strategies on classic vehicle routing problem with time windows(VRPTW) benchmark instances and the TDVRPTW instances, and some better results especially for the number of vehicles and travel times of the best solutions are obtained in comparison with the previous research. 展开更多
关键词 multi-type ant system(MTAS) time dependent vehicle routing problem with time windows(VRPTW) nearest neighbor selection(NNS)
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Effects of Seed Maturity of Turf-Type Tall Fescue on the Seed Vigor and the Optimal Harvesting Time 被引量:3
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作者 XU Rong and HAN Jian-guoInstitute of Grassland Science, China Agricultural University, Beijing 100094 , P.R.China 《Agricultural Sciences in China》 CAS CSCD 2003年第4期454-462,共9页
By assay of accelerated aging germination, germination index, vigor index, seedling length, seedling weight, electric conductivity, dehydrogenase activity, ATP content and acid phosphoesterase activity during seed dev... By assay of accelerated aging germination, germination index, vigor index, seedling length, seedling weight, electric conductivity, dehydrogenase activity, ATP content and acid phosphoesterase activity during seed development of turf-type tall fescue, the seed vigor of tall fescue were studied. Combining with seed moisture content and yield, the optimal harvesting time of tall fescue was determined. The results indicated that the seed vigor increased continuously along with seed maturity, and the higher seed vigor was achieved at 19th day after perk anthesis and maintained continuously until 31st day after peak anthesis. At 25th day after peak anthesis, the highest yield of 3 533 kg ha-1 and the good quality of seeds of tall fescue were harvested with 32.19% moisture content. From 22nd to 31st day after peak anthesis, the seed yield of 3 300 kg ha-1 and the good quality seeds of tall fescue were harvested with 40 - 12. 43% moisture content, and the span was the optimal harvesting time. 展开更多
关键词 Turf-type tall fescue Seed vigor Optimal harvesting time
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Integral Inequality of Hardy-Type on Time Scales
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作者 Emmanuella Ehui Aribike Yisa Oluwatoyin Anthonio +1 位作者 Kamilu Rauf Michael Oyelami Ajisope 《Advances in Pure Mathematics》 2019年第6期534-543,共10页
Inequalities are essential in the study of Mathematics and are useful tools in the theory of analysis. They have been playing a critical role in the study of the existence and uniqueness properties of solutions of ini... Inequalities are essential in the study of Mathematics and are useful tools in the theory of analysis. They have been playing a critical role in the study of the existence and uniqueness properties of solutions of initial and boundary value problems for differential equations as well as difference equations with their bounds. In this paper, we obtain new integral inequalities mainly by using some known inequalities. Various generalizations of Hardy's inequality are special cases of the results therein. 展开更多
关键词 time Scale Measurable CONVEX FUNCTIONS Hardy-type INEQUALITIES
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Bianchi Type-IX Anisotropic Dark Energy Cosmological Models with Time Dependent Deceleration Parameter
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作者 H. R. Ghate Atish S. Sontakke Yogendra D. Patil 《International Journal of Astronomy and Astrophysics》 2015年第4期302-323,共22页
Bianchi type-IX cosmological models with variable equation of state (EoS) parameter have been investigated in general relativity when universe is filled with dark energy. The field equations have been solved by consid... Bianchi type-IX cosmological models with variable equation of state (EoS) parameter have been investigated in general relativity when universe is filled with dark energy. The field equations have been solved by considering (i) q=B (variable);(ii) , where k and m are constants;(iii) , where k is constant and R is average scale factor;(iv) which gives . This renders early decelerating and late time accelerating cosmological models. The physical and geometrical properties of the models are also discussed. 展开更多
关键词 DARK Energy DECELERATION Parameter Bianchi type-IX Space time
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Numerical Solutions for the Time-Dependent Emden-Fowler-Type Equations by B-Spline Method
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作者 Mohamed El-Gamel W. El-bashbashy Atallah El-Shenawy 《Applied Mathematics》 2014年第4期593-600,共8页
A numerical method based on B-spline is developed to solve the time-dependent Emden-Fow- ler-type equations. We also present a reliable new algorithm based on B-spline to overcome the difficulty of the singular point ... A numerical method based on B-spline is developed to solve the time-dependent Emden-Fow- ler-type equations. We also present a reliable new algorithm based on B-spline to overcome the difficulty of the singular point at x = 0. The error analysis of the method is described. Numerical results are given to illustrate the efficiency of the proposed method. 展开更多
关键词 B-SPLINE time-DEPENDENT Numerical Solutions EMDEN-FOWLER type
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日常时间管理量表的汉化及信效度检验
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作者 马旖旎 张理想 +1 位作者 赵俐丽 史天陆 《护理研究》 北大核心 2025年第4期617-623,共7页
目的:将日常时间管理量表(Daily Time Management Scale,DTMS)进行汉化,并对其进行信效度检验。方法:遵循Brislin翻译模式进行量表翻译,通过专家咨询和预调查进行文化调适后形成中文版DTMS。采用便利抽样法,选取300例2型糖尿病病人进行... 目的:将日常时间管理量表(Daily Time Management Scale,DTMS)进行汉化,并对其进行信效度检验。方法:遵循Brislin翻译模式进行量表翻译,通过专家咨询和预调查进行文化调适后形成中文版DTMS。采用便利抽样法,选取300例2型糖尿病病人进行横断面调查,分析中文版DTMS的信效度。结果:中文版DTMS项目分析结果显示所有条目均设计合理、区分度良好。量表条目水平的内容效度指数(I-CVI)为0.875~1.000,量表内容效度指数(S-CVI)为0.928。探索性因子分析共提取3个公因子,累计方差贡献率为64.695%。总量表的Cronbach's α系数为0.864,3个维度的Cronbach's α系数分别为0.851,0.834,0.746;重测信度为0.845。中文版DTMS包括工作满意度与生活充实度的协调、生活节奏调整和全面考量的目标取向3个维度,共16个条目。结论:中文版DTMS在2型糖尿病病人中的应用具有良好的信效度,可作为评估2型糖尿病病人日常时间管理的有效工具。 展开更多
关键词 日常时间管理 2型糖尿病 量表 信度 效度
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Global Existence and Large Time Asymptotic Behavior of Strong Solution to the Cauchy Problem of 2D Density-Dependent Boussinesq Equations of Korteweg Type
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作者 Qi Zhang 《Advances in Pure Mathematics》 2021年第4期346-368,共23页
In this paper, we study the Cauchy problem of the density-dependent Boussinesq equations of Korteweg type on the whole space with a vacuum. It is proved that there exists a unique strong solution for the two-dimension... In this paper, we study the Cauchy problem of the density-dependent Boussinesq equations of Korteweg type on the whole space with a vacuum. It is proved that there exists a unique strong solution for the two-dimensional Cauchy problem established that the initial density and the initial temperature decay not extremely slow. Particularly, it is allowed to be arbitrarily large for the initial data and vacuum states for the initial density, even including the compact support. Moreover, when the density depends on the Korteweg term with the viscosity coefficient and capillary coefficient, we obtain a consistent priority estimate by the energy method, and extend the local strong solutions to the global strong solutions. Finally, when the pressure and external force are not affected, we deform the fluid models of Korteweg type, we can obtain the large time decay rates of the gradients of velocity, temperature and pressure. 展开更多
关键词 Incompressible Boussinesq Equation Korteweg type Global Strong Solutions Large time Behavior Vacuum
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水产养殖轨道式精准饲料投喂系统设计与试验 被引量:1
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作者 朱明 孙勇健 +3 位作者 雷翔 牛鹏基 赵振鹏 万鹏 《渔业现代化》 北大核心 2025年第2期78-89,共12页
针对水产养殖过程中饲料投喂劳动强度大、投喂不均匀、效率低等问题,设计了一款基于地轨的轨道式精准饲料投喂系统,整套系统集机械结构设计、自动控制系统、上位机监视投喂信息管理系统于一体。通过对系统的行走装置、料仓、下料装置、... 针对水产养殖过程中饲料投喂劳动强度大、投喂不均匀、效率低等问题,设计了一款基于地轨的轨道式精准饲料投喂系统,整套系统集机械结构设计、自动控制系统、上位机监视投喂信息管理系统于一体。通过对系统的行走装置、料仓、下料装置、称重装置等主要关键部件进行设计与理论分析,确定了系统的结构参数,基于SIEMENS S7-200 SMART PLC开发了自动控制系统,并以行驶速度、定位精度、投喂速度、投喂精度、饲料破碎率为试验指标进行饲料投喂试验。结果显示:系统运行稳定可靠,可以顺利自动启停,行驶速度为12.7 m/min,定位精度误差范围在39~58 mm,投喂速度为3.31 kg/min,投喂精度误差<0.63%,饲料破碎率低于1%。整个投喂全程自动化运行,上位机能够实时监视系统的行驶过程和投喂过程。研究表明,该系统提高了饲料利用率,降低了劳动成本和投饲成本,同时一体化的投饲设备能更好管理使用,为水产养殖自动化提供了可行方案。 展开更多
关键词 轨道式投饲系统 水产养殖 定位 定时定量 PLC 精准投喂
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中国内地最大余震的统计特征分析
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作者 陈大庆 邵叶 +3 位作者 许斌斌 杨雪 王小娜 仲秋 《华南地震》 2025年第3期6-12,共7页
对1976年以来中国内地134次6级以上地震的破裂类型、序列类型、主震与最大余震的震级差、时间间隔以及序列类型和主震前震中区域中小地震活动的关系等进行了统计分析,发现90%的最大余震发生在主震后90天以内。按破裂类型划分,各类型中... 对1976年以来中国内地134次6级以上地震的破裂类型、序列类型、主震与最大余震的震级差、时间间隔以及序列类型和主震前震中区域中小地震活动的关系等进行了统计分析,发现90%的最大余震发生在主震后90天以内。按破裂类型划分,各类型中主余型的占比都超过了60%,并且相对在走滑型和正断型中占比较更高,而孤立型在走滑型中占比较低,正断型中甚至未出现孤立型地震,并且孤立型地震多发生在地震相对不活跃的区域,随着主震震级的升高,孤立型序列的数量占比显著减小。统计主震后24h内最大余震和后续最大余震的关系发现,主震后24h内发生的最大余震越大,则后续最大余震越小。如果24h内最大余震和主震震级差1级以内或者2.5级以上,前者后续发生更大余震可能性比例较小为18.2%,后者则可能性很大为89.5%。这些统计规律可以在震前、震后不同时间段对序列的最大余震做出估计。 展开更多
关键词 中国内地 最大余震 序列类型 破裂类型 间隔时间
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传送带电机系统的显式时间区间二型模糊控制
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作者 孟瑾 崔建华 +2 位作者 朱丹 高振华 严文 《制造业自动化》 2025年第5期39-45,共7页
具有波动流量的传送带电机系统在数学上可以描述为具有扰动的切换系统。但在实际工程中,扰动的不确定性会影响输送过程的完成时间。因此,为了解决这类未知扰动下切换控制系统的稳定时间先验性问题,提出了一种显式时间自适应区间二型模... 具有波动流量的传送带电机系统在数学上可以描述为具有扰动的切换系统。但在实际工程中,扰动的不确定性会影响输送过程的完成时间。因此,为了解决这类未知扰动下切换控制系统的稳定时间先验性问题,提出了一种显式时间自适应区间二型模糊控制方法。首先,将传送带电机系统描述为具有时间切换关系的系统。然后,根据系统的显式时间稳定性设计了鲁棒控制器。最后,为了处理不确定流量波动引起的扰动,采用自适应区间型模糊逻辑系统作为鲁棒项增益来逼近这种不确定性。与现有的相关控制方法相比,所提方法可以实现未知扰动下传送带电机切换系统的显式时间稳定性,并且控制输入更低、更平滑。仿真和实验验证了该方法的有效性。 展开更多
关键词 传送带电机系统 显式时间稳定性 区间二型模糊控制
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考虑双时间窗特性的机场多车型摆渡车调度优化
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作者 张文义 唐雨拉尔 +3 位作者 王旭兰 周静 边可 刘志硕 《北京航空航天大学学报》 北大核心 2025年第10期3345-3353,共9页
为提升摆渡车运行的灵活性、增强车辆调度方案的能效和实践可操作性,综合考虑多车型和机场摆渡车运输服务的双时间窗特性,从任务点视角构建机场摆渡车调度问题的混合整数线性规划模型。该模型可视为一个考虑多车型和双时间窗且需求可拆... 为提升摆渡车运行的灵活性、增强车辆调度方案的能效和实践可操作性,综合考虑多车型和机场摆渡车运输服务的双时间窗特性,从任务点视角构建机场摆渡车调度问题的混合整数线性规划模型。该模型可视为一个考虑多车型和双时间窗且需求可拆分的车辆路径问题,是一个复杂的NP-hard问题,为此,提出一种改进的自适应大邻域搜索(ALNS)算法。以北京首都国际机场东飞行区为背景的案例仿真结果表明:所提算法具有良好的稳定性和优化效果,200次迭代下10轮次计算实验结果的标准差与平均值之比仅为5.6%,优化前后对应的最优目标函数值下降达54%;多车型调度方案显著优于人工和单一车型调度方案,在车辆容量利用能效上较人工、大车型和小车型方案分别提升了98.3%、31.3%和22.2%,在运行总成本上较人工、大车型和小车型方案则分别下降了48.3%、23.4%和23.5%。 展开更多
关键词 航空运输 机场 摆渡车调度 多车型 双时间窗 自适应大邻域搜索
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分数阶中立型时变时滞神经网络的全局渐近稳定性
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作者 刘国权 周吴昊 +1 位作者 陈立平 周书民 《控制理论与应用》 北大核心 2025年第6期1170-1180,共11页
本文研究了一类分数阶中立型时变时滞神经网络的全局渐近稳定性问题.首先,本文提出了一类含有时变时滞的分数阶中立型神经网络模型,该模型含有分数阶微分项和时变时滞;其次,基于Lyapunov泛函理论、线性矩阵不等式方法,本文深入研究了该... 本文研究了一类分数阶中立型时变时滞神经网络的全局渐近稳定性问题.首先,本文提出了一类含有时变时滞的分数阶中立型神经网络模型,该模型含有分数阶微分项和时变时滞;其次,基于Lyapunov泛函理论、线性矩阵不等式方法,本文深入研究了该模型的全局渐进稳定性问题,推导出了新的该模型的稳定性判据;最后,通过4个实例验证了所得的稳定性判据的可行性.本文研究为含有时变时滞的分数阶中立型神经网络的稳定性研究提供了新的依据. 展开更多
关键词 稳定性 分数阶中立型神经网络 LYAPUNOV-KRASOVSKII泛函 时变时滞 线性矩阵不等式
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基于混合模型的多类型机场航班过站时间预测 被引量:1
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作者 李国 王伟倩 曹卫东 《计算机工程与设计》 北大核心 2025年第2期633-640,F0003,共9页
为更精确地预测航班过站时间,将全国机场按照规模差异及不同地理位置所导致的客流量差异和天气差异对航班过站时间造成的不同影响进行分类,基于各类机场航班数据,构建混合轻量级梯度提升机算法(LightGBM)模型对航班过站时间分类预测。... 为更精确地预测航班过站时间,将全国机场按照规模差异及不同地理位置所导致的客流量差异和天气差异对航班过站时间造成的不同影响进行分类,基于各类机场航班数据,构建混合轻量级梯度提升机算法(LightGBM)模型对航班过站时间分类预测。引入自适应鲁棒损失函数(adaptive robust loss function,ARLF)改进LightGBM模型损失函数,降低航班数据中存在离群值的影响;通过改进的麻雀搜索算法对改进后的LightGBM模型进行参数寻优,形成混合LightGBM模型。采用全国2019年全年航班数据进行验证,实验结果验证了方法的可行性。 展开更多
关键词 多类型机场 航班过站时间预测 客流量差异 天气差异 混合轻量级梯度提升机算法模型 自适应鲁棒损失函数 离群值 麻雀搜索算法
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猪IL-4、IL-6和IL-10 SYBR Green Ⅰ real-time PCR检测方法的建立及应用 被引量:11
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作者 施开创 李焕荣 +2 位作者 杨汉春 郭鑫 盖新娜 《中国预防兽医学报》 CAS CSCD 北大核心 2010年第2期102-107,共6页
为探讨PRRSV感染后机体的体液免疫应答、从分子水平深入研究PRRSV的免疫机制,本研究针对Th2型细胞因子IL-4、IL-6、IL-10以及看家基因β-actin的基因序列分别设计一对特异性引物,构建含有各自引物扩增序列的重组质粒作为阳性标准品,建... 为探讨PRRSV感染后机体的体液免疫应答、从分子水平深入研究PRRSV的免疫机制,本研究针对Th2型细胞因子IL-4、IL-6、IL-10以及看家基因β-actin的基因序列分别设计一对特异性引物,构建含有各自引物扩增序列的重组质粒作为阳性标准品,建立了检测IL-4、IL-6、IL-10及β-actin的SYBR Green Ⅰ real-time PCR方法。该方法线性关系好,各种细胞因子及β-actin标准曲线的相关系数均达到0.997以上;敏感性高,初始模板的检出下限均达到1×101copies/μL;特异性强,扩增产物形成单一的特异性熔解峰;重复性好,组内与组间的变异系数均小于3%。应用所建立的方法对猪繁殖与呼吸综合征病毒(PRRSV)感染仔猪外周血单个核细胞(PBMC)中IL-4、IL-6和IL-10mRNA的表达水平进行了检测。结果表明,本研究建立的real-timePCR检测方法灵敏度高、特异性强、重复性好,可以用于猪Th2型细胞因子的检测及定量分析。 展开更多
关键词 TH2型细胞因子 荧光定量PCR 检测方法
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