目的验证TIDE(Track of Indels by Decomposition)和TIDER(Tracking of Insertion,DEletions and Recombination events)分析绵羊囊胚基因组编辑结果的可靠性,为检测基因组编辑绵羊胚胎突变效率提供精准、及时的分析方法。方法TIDE是1...目的验证TIDE(Track of Indels by Decomposition)和TIDER(Tracking of Insertion,DEletions and Recombination events)分析绵羊囊胚基因组编辑结果的可靠性,为检测基因组编辑绵羊胚胎突变效率提供精准、及时的分析方法。方法TIDE是1种简便精准的检测方法,能精确测定CRISPR/Cas9在细胞群体中引发的靶向突变谱型和频率。利用TIDE平台对绵羊囊胚的基因组诱导突变进行表征分析与量化。结果以MSTN基因突变绵羊的基因组样本为材料,进行PCR扩增并构建T载体,随机选取单克隆测序。测序结果证实了TIDE分析基因组编辑结果的准确性。通过将TIDER分析单链寡核苷酸模板整合产生的编辑结果与DNA限制性酶切图谱对比,验证了TIDER分析外源DNA短片段导入的准确性。结论TIDE和TIDER工具可有效量化绵羊囊胚突变率,识别主要的插入和缺失类型,是高效经济的基因突变分析方法。展开更多
Accurate knowledge of mesospheric winds and waves is essential for studying the dynamics and climate in the mesosphere and lower thermosphere(MLT)region.In this study,we conduct a comparative analysis of the mesospher...Accurate knowledge of mesospheric winds and waves is essential for studying the dynamics and climate in the mesosphere and lower thermosphere(MLT)region.In this study,we conduct a comparative analysis of the mesosphere tidal results obtained from two adjacent meteor radars at low latitudes in Kunming,China,from November 2013 to December 2014.These two radars operate at different frequencies of 37.5 MHz and 53.1 MHz,respectively.However,overall good agreement is observed between the two radars in terms of horizontal winds and tide observations.The results show that the dominant tidal waves of the zonal and meridional winds are diurnal and semidiurnal tides.Moreover,we conduct an exhaustive statistical analysis to compare the tidal amplitudes and vertical wavelengths recorded by the dual radar systems,which reveals a high degree of alignment in tidal dynamics.The investigation includes variances and covariances of tidal amplitudes,which demonstrate remarkable consistency across measurements from both radars.This finding highlights clear uniformity in the mesospheric tidal patterns observed at low latitudes by the two neighboring meteor radars.Results of the comparative analysis specifically underscore the significant correlation in vertical wavelength measurements,validating the robustness of radar observations for tidal research.展开更多
We use a high-resolution numerical simulation to analyze the generation and evolution of semidiurnal internal tides in the Weddell-Scotia Confluence.Our results indicate that two ridges near the Antarctic Peninsula,th...We use a high-resolution numerical simulation to analyze the generation and evolution of semidiurnal internal tides in the Weddell-Scotia Confluence.Our results indicate that two ridges near the Antarctic Peninsula,the South Scotia Ridge(SSR)and the Philip Ridge(PR),are energetic sources of semidiurnal internal tides.The strongest semidiurnal barotropic to baroclinic energy conversion occurs around the crest of the SSR,reaching 0.3 W/m^(2).The depthintegrated,tidally averaged semidiurnal internal tide energy fluxes that radiated from the SSR reach about 2 kW/m.The northward semidiurnal internal tide energy fluxes entering the Scotia Sea are stronger than the southward energy fluxes entering the Powell Basin.For the SSR region between the South Orkney Plateau and Elephant Island,the areaintegrated barotropic to baroclinic conversion rate is 0.71 GW,of which 0.56 GW(79%)dissipates locally.The dissipation of internal tides occurs mainly in water depths of less than 1000 m.The dissipation rate over the SSR is as high as 10−7 W/kg.The energy fluxes create an anticlockwise gyre between the SSR and PR.The horizontal kinetic energy and available potential energy oscillate sinusoidally with peaks occurring at quarter-wavelength intervals.This energy pattern suggests the formation of standing waves due to interference between the semidiurnal internal tides originating from the SSR and PR.The results of this study show that internal tides and related mixing need to be considered in the analysis of water mass transformation in the Southern Ocean.展开更多
Red tide is an ecological disaster caused by the excessive proliferation of photosynthetic algae in the ocean.The frequent occurrences of red tide have brought serious harms to the marine aquaculture and caused signif...Red tide is an ecological disaster caused by the excessive proliferation of photosynthetic algae in the ocean.The frequent occurrences of red tide have brought serious harms to the marine aquaculture and caused significant economic losses to the marine industry.Red tide prediction can alleviate and even stop the long-term damages to marine ecosystems,which helps maintain the ecological balance of the ocean environment and contributes to the Sustainable Development Goal of“life below water”formulated by the United Nations.Aiming at red tide prediction using remote sensing technology,this study proposed a novel approach of red tide prediction using time-series hyperspectral observations,and examined the proposed method in the Xinghai Bay,China.Three spectral indices,namely the twoband ratio(TBR),the three-band spectral index(TBSI),and the fluorescence baseline height(FLH),were used to reduce the dimensionality of hyperspectral data and extract spectral features.Two machine learning models including the random forest(RF)and the support vector machine(SVM)were employed to predict whether red tide would occur on a target day based on the time-series spectral indices obtained in the previous days.By comparing and analyzing the prediction results of multiple machine learning models trained with different spectral indices and temporal lengths,it is found that both the RF and the SVM models can predict the red tide outbreaks at the accuracies over 0.9 using adequate temporal lengths of input data.When the temporal length of input data is limited,however,it is suggested to use the RF model,which accurately predicts red tide outbreaks using the temporal input of the 2-d TBSI.The proposed method is expected to provide oceanic and maritime agencies with early warnings on red tide outbreaks and ensure the safety of the coastal environment in large spatial scales using optical remote sensing technology.展开更多
Ocean mixing is a consequence of essential dynamic processes such as internal tides and lee waves that occur near the seafloor topography.Internal tides and lee waves are generated by barotropic tidal currents and geo...Ocean mixing is a consequence of essential dynamic processes such as internal tides and lee waves that occur near the seafloor topography.Internal tides and lee waves are generated by barotropic tidal currents and geostrophic flows,respectively.Ocean current is composed of multiple flows;thus,internal tides and lee waves occur concurrently in the real ocean.In this paper,the Massachusetts Institute of Technology general circulation model(MITgcm)is used to conduct 2D numerical experiments.By varying background flow intensities,the energy and dissipation relationship between internal tides and lee waves are investigated.The results reveal that the internal tide beams become asymmetric due to the influence of Doppler shift.The lee wave structure gradually leads the wave field when the background flow velocity rises constantly.The presence of a background flow increases the energy portion of the high-mode wave by up to 15%-20%.Moreover,strong shear,owing to the background flow,considerably increases dissipation.When the background flow velocity is higher than the barotropic tidal current velocity,the isopycnal overturn triggered by the lee wave generates a dissipation of the same order of magnitude as the shear.展开更多
As a frequently occurred marine pollution phenomenon,red tides of water body due to eutrophication cause massive mortality of marine organisms and serious ecological problems.The early warning and prediction of red ti...As a frequently occurred marine pollution phenomenon,red tides of water body due to eutrophication cause massive mortality of marine organisms and serious ecological problems.The early warning and prediction of red tide outbreak can provide guidance to the coastal management,and is of great value to the aquaculture industry and marine environment protection.An approach for the risk assessment of red tide occurrence using spectral indices was made.The optimal spectral indices were explored from three candidates,namely two-band ratio(TBR)method,three-band spectral index(TBSI)method,and fluorescence baseline(FLB)method.The correlations between the spectral indices and the red tide occurrence were quantitatively evaluated through analysis of variance(ANOVA).The risk maps for the Beibu Gulf and the Bohai Bay in China were produced with the normalized spectral indices based on the multi-spectral observation from Sentinel-3 satellite.Results show that both TBR and TBSI values have significant correlations with the occurrences of red tide as the ANOVA results.TBSI illustrated correctly the risk of red tide occurrence in the risk maps and was the optimal spectral index offshore risk assessment of red tide.FLB method failed to recognize the high-risk regions and may not be the appropriate spectral index.The risk assessment method proposed in this study can provide early alarms on red tide occurrence and help timely the countermeasure against potential harms.展开更多
Ulva prolifera green tides are becoming aworldwide environmental problem,especially in the Yellow Sea,China.However,the effects of the occurrence of U.prolifera green tides on the community organization and stability ...Ulva prolifera green tides are becoming aworldwide environmental problem,especially in the Yellow Sea,China.However,the effects of the occurrence of U.prolifera green tides on the community organization and stability of surrounding microbiomes have still not been de-termined.Here,the prokaryotic microbial community network stability and assembly char-acteristics were systematically analyzed and compared between the green tide and non-green tide periods.U.prolifera blooms weaken the community complexity and robustness of surrounding microbiomes,increasing fragmentation and decreasing diversity.Bacteria and archaea exhibited distinct community distributions and assembly patterns under the influ-ence of green tides,and bacterial communities were more sensitive to outbreaks of green tides.The bacterial communities exhibited a greater niche breadth and a lower phyloge-netic distance during the occurrence of U.prolifera green tides compared to those during the non-green tide period while archaeal communities remained unchanged,suggesting that the bacterial communities underwent stronger homogeneous selection and more sensitive to green tide blooms than the archaeal communities.Piecewise structural equation model analysis revealed that the different responses of major prokaryotic microbial groups,such as Cyanobacteria,to environmental variables during green tides,were influenced by the variations in pH and nitrate during green tides and correlated with the salinity gradient during the non-green tide period.This study elucidates the response of the adaptability,associations,and stability of surrounding microbiomes to outbreaks of U.prolifera green tides.展开更多
Motivated by the wide applications of empirical global ocean tide models in Earth science,particularly in shallow waters and coastal regions,we proposed an updated global ocean tide model representing all major diurna...Motivated by the wide applications of empirical global ocean tide models in Earth science,particularly in shallow waters and coastal regions,we proposed an updated global ocean tide model representing all major diurnal and semidiurnal tidal constituents.We integrated the development technique of the Technical University of Denmark(DTU10)model and calculated the residual tides using the intermediary FES2012 model.We utilized all available Topex/Poseidon,Jason-1,Ocean Surface Topography Mission(OSTM)/Jason-2 primary and tandem missions to develop the new model.To reduce the discrepancies between the model and in situ measurements estimated S2 tide constituent,the ERA-Interim model was selected for dry tropospheric correction of altimetry data.The newly developed model with an improved spatial resolution from 1/8°×1/8°to 1/16°×1/16°was assessed and compared against contemporary global ocean tide models using in situ measurements in coastal regions,continental shelf waters,marginal seas,and deep waters.The results demonstrate improvements in shallow waters and coastal regions,particularly in representative coastal re-gions such as the Northwest European Shelf and East Asian Marginal Seas.Moreover,the model performs well in simulating S_(2) and K_(2) tides with root mean square differences of 0.355 and 0.234 cm,respectively,against in situ measurements in deep waters world-wide.Compared with that of DTU10,the root sum square of the new model for the eight main tidal constituents decrease by 8.4%to 0.997 cm.展开更多
This study on the distribution of phosphate and its relation to phytoplankton biomass in Western Xiamen Harbor using marine ecosystem enclosures to isolate the culture water from the tidal currents and salinity change...This study on the distribution of phosphate and its relation to phytoplankton biomass in Western Xiamen Harbor using marine ecosystem enclosures to isolate the culture water from the tidal currents and salinity changes outside indicated that the phytoplankton biomass variation closely related to dissolved inorganic phosphorus (DIP) in the seawater as described by the equation: [Chl a]=A×e -B[PO4] . The biomass changes lagged by about two days the corresponding DIP. The research also dealt with the minimal DIP concentration for stopping diatom bloom and the possible maximal diatom biomass was estimated from the DIP external concentration in the seawater. The threshold of DIP initiating Skeletonema costatum red tide was calculated for use as an index to forecast its red tides. In addition, the relationships between a dinoflagellate red tide and nutrients are discussed. The results showed that the multiplication of dinoflagellate was not entirely dependent on the nutrients in the seawater.展开更多
A series of enclosed ecosystem experiments were conducted in a land based tank near the seaside of West Xiamen Harbor.The results of experiments conducted in different seasons and years showed a repeatble phytoplankto...A series of enclosed ecosystem experiments were conducted in a land based tank near the seaside of West Xiamen Harbor.The results of experiments conducted in different seasons and years showed a repeatble phytoplankton succession. In this relatively stable ecosystem with added nutrients and trace metals,diatoms dominated initially,dinoflagellates dominated in the later stage,and dinoflagellate red tides eventually occurred.Vitamin B 12 enrichment may speed up this succession process. Stirring the water column could stop this process. Soluble Mn at a level of 3-4 μg/L in seawater,which also is the existing concentration of solu ble Mn in Xiamen Harbor seawater,is sufficient for the multiplication of algae and occurrence of red tide.The present study showed that excessive soluble Mn in Xiamen Harbor cannot cause red tide,and that Fe was one of the important factors causing diatom red tide in this present study..展开更多
To study the Taiwan Strait （TS）, an unusual sea area, the numerical model in marginal seas of China is used to simulate and analyze the tidal wave motion in the strait. The numerical modeling experiments reproduce t...To study the Taiwan Strait （TS）, an unusual sea area, the numerical model in marginal seas of China is used to simulate and analyze the tidal wave motion in the strait. The numerical modeling experiments reproduce the amphidromic system of the M2 tide in the south end of the Taiwan strait, and consequently confirm the existence of the degenerate amphidromic system. On this basis, further discussion is conducted on the M2 system and its formation mechanism. It can be concluded that the tidal waves of the TS is consisted of the progressing wave from the north entrance and the degenerate amphidromic system from the south entrance, in which the progressing wave from the north entrance dominates the tidal wave motion in the strait. Except for the convergent effect caused by the landform and boundary, the degenerate amphidromic system produced in the south of the strait is another important factor for the following phenomena： the large tidal range in the middle of the strait, the concentrative zone of co-amplitude and co-phase line in the south of the strait. The degenerate amphidromic system is mainly produced by the incident Pacific Ocean tidal wave from the Luzon strait and the action by the shoreline and landform. The position of the amphidromic point is compelled to move toward southwest until degenerating by the powerful progressing wave from the north entrance.展开更多
In the numerical studies of a real tide M4 resonance system, the Xiangshan Port which is a partially-closed bay, Dong et al. [1999. Acta Oceanologica Sinica, 21 （3）： 1-6] found the interesting phenomenon that the a...In the numerical studies of a real tide M4 resonance system, the Xiangshan Port which is a partially-closed bay, Dong et al. [1999. Acta Oceanologica Sinica, 21 （3）： 1-6] found the interesting phenomenon that the advection plays an important role in inhibiting the growth of the amplitude of the tidal second-order resonance response （M4）. This result is contrary to the general traditional ideas for a non-resonance system. How this phenomenon is interpreted and what internal mechanism is behind the phenomenon are the main focuses of this study. The followings are examined： （1） the dynamic features of a second-order resonance system of tide; （2） the dominating factors on the second-order resonance responses; （3） the effects of both the friction and the advection on the second-order resonance responses; and （4） their roles in dominating the second-order resonance response and internal mechanisms by using the analytical methods. The respective results show that： （1） Both the bottom friction and the advection play significant roles in dominating the magnitude of the amplitude of the second-order resonance responses; （2） the effect of the friction on the second-order resonance response depends on the distribution ratio of the work-done of the system to friction force exhausted into between the damping of the first-order system and the inner excitation of the second-order system; （3） the advection plays a positive role in increasing the amplitude of the second-order non-resonance response in the second order non-resonance of tide; （4） in a second-order resonance system of tide, the effect of the advection may be either to increase or to decrease the amplitudes of the second-order resonance responses of tide, which depends on the distribution ratio mentioned above.展开更多
Green tides have occurred every year from 2007 to 2014 in the Yellow Sea. Ulva prolifera （Mtiller） J. Agardh has been identified as the bloom-forming alga, co-occurring with U. intestinalis. We observed distinct str...Green tides have occurred every year from 2007 to 2014 in the Yellow Sea. Ulva prolifera （Mtiller） J. Agardh has been identified as the bloom-forming alga, co-occurring with U. intestinalis. We observed distinct strategies for both algal species during green tides. U.prolifera exhibited a high abundance initially and then decreased dramatically, while U. intestinalis persisted throughout. The antioxidant system responses of these two macroalgae were compared in the late phase of a green tide （in-situ） and after laboratory acclimation. Lipid peroxidation and antioxidant system responses differed significantly between the two. Malondialdehyde and hydrogen peroxide contents increased significantly in-situ in U. prolifera, but not in U. intestinalis. In U. prolifera, we observed a significant decrease in total antioxidant ability （T-AOC）, antioxidant enzymes （SOD and Apx）, and non-enzyme antioxidants （GSH and AsA） in-situ. U. intestinalis showed the same pattern of T-AOC and SOD, but its Gpx, Apx, and GSH responses did not differ significantly. The results suggest that U. prolifera was more susceptible than U. intestinalis to the harsh environmental changes during the late phase of a Yellow Sea green tide. The boom and bust strategy exhibited by U. prolifera and the persistence of U. intestinalis can be explained by differences in enzyme activity and antioxidant systems.展开更多
The Brazilian coast is characterized by different tidal regimes and distinct meteorological influences. The northern part has larger tidal amplitudes and is permanently affected by trade winds and tropical disturbance...The Brazilian coast is characterized by different tidal regimes and distinct meteorological influences. The northern part has larger tidal amplitudes and is permanently affected by trade winds and tropical disturbances; the southern portion has smaller tidal amplitudes and is frequently influenced by extratropical cyclone activity. Besides these aspects, many features regarding current structure and behavior are also present, such as the equatorial system of currents, the subtropical gyre and the corresponding western boundary currents, and the Brazil-Malvinas confluence region. Within this context, efforts were made to develop the BRAZCOAST system, capable of describing the processes that determine the oceanic circulation from large to coastal scales. A customized version of the Princeton Ocean Model(POM) was implemented in a basin-scale domain covering the whole of the tropical and southern Atlantic Ocean, with 0.5° spatial resolution, as well as three nested grids with(1/12)° resolution covering the different parts of the Brazilian shelf, in a one-way procedure. POM was modified to include tidal potential generator terms and a partially-clamped boundary condition for tidal elevations. The coarse grid captured large-scale features, while the nested grids detailed local circulations affected by bathymetry and coastal restrictions. An interesting aspect at the coarse grid level was the relevance of the Weddell Sea to the location of the tidal amphidromic systems.展开更多
This study was performed to compare storm surges/tide simulated by the regional and coastal storm surges/tide forecast system （RTSM （regional tide/storm surges model）, CTSM （coastal tide/storm surges model）） usi...This study was performed to compare storm surges/tide simulated by the regional and coastal storm surges/tide forecast system （RTSM （regional tide/storm surges model）, CTSM （coastal tide/storm surges model）） using two different inputs from weather models （RDAPS （Regional Data Assimilation and Prediction System） and KWRF （Korea Weather and Research Forecasting）） during two typhoons that occurred between 2007 and 2008. Both the RDAPS and KWRF are the operational weather forecasting system in KMA （Korea Meteorological Administration）. The horizontal resolutions of RDAPS and KWRF are 30 and 10 km, respectively. The storm surges/tide was hind casted using sea wind and pressure fields of two Typhoons which was approaching Korean Peninsula. The CTSM using input from KWRF simulate very well the storm surges/tide pattern in the complex coastal areas. The result showed that the storm surges by the coastal storm surges/tide model with high resolution input was in well agreement with the observed sea level occurred by high tide and storm surges in the coastal areas.展开更多
Since 2007,the large-scale green tide caused by Ulva prolifera(U.prolifera)have occurred as a recurrent phenomenon in the southern Yellow Sea of China.Field surveys and satellite remote sensing showed that the small s...Since 2007,the large-scale green tide caused by Ulva prolifera(U.prolifera)have occurred as a recurrent phenomenon in the southern Yellow Sea of China.Field surveys and satellite remote sensing showed that the small scattered patches of green tide algae were first observed along the Porphyra agriculture area of the Subei Shoal in late April.In this study,we attempted to identify the role of eutrophication in the origin of the green tide in the Subei Shoal and its adjacent area.Subei Shoal and its adjacent area are characterized by rich nutrients,especially NO_(3)^(-)-N,NH_(4)^(+)-N,PO_(4)^(3-)-P,and other bioavailable components(such as urea-N and amino acids).In the spring of 2017,the average concentrations of NO_(3)^(-)-N were 19.01±11.01μmolL^(-1),accounting for 86.68%of the dis-solved inorganic nitrogen(DIN).In addition,the average concentration of NH4^(+)-N was 2.51±1.60μmolL^(-1).PO_(4)^(3-)-P had an average concentration of 0.14±0.13μmolL-1.The average concentrations of urea-N and total hydrolyzed amino acids(THAA)were 1.73±1.36μmolL^(-1)and 1.33±0.80μmolL^(-1),respectively.Rich nutritive substances play a key role in the rapid production of U.prolifera and make the Jiangsu coastal water an incubator for green tide.展开更多
Ulva prolifera is the causative species of the annually occurring large-scale green tides in China since 2007.Its specific biological features on reproductivity strategies,as well as intra-species genetic diversity,ar...Ulva prolifera is the causative species of the annually occurring large-scale green tides in China since 2007.Its specific biological features on reproductivity strategies,as well as intra-species genetic diversity,are still largely unknown,especially at the genome level,despite their importance in understanding the formation and outbreak of massive green tides.In the present study,the restriction site-associated DNA genotyping approach(2b-RAD)was adopted to identify the genome-wide single-nucleotide polymorphisms(SNPs)of 54 individual thalli including samples collected from Subei Shoal in 2019 and Qingdao coast from 2019 to 2021.SNPs genotype results revealed that most of the thalli in 2019 and 2020 were haploid gametophytes,while only half of the thalli were gametophytes in 2021,indicating flexibility in the reproductive strategies for the formation of the green tides among different years and the dominance of asexual and vegetative reproductive mode for the floating period.Besides,population analysis was conducted,and it revealed a very low genetic diversity among samples from Subei Shoal and the Qingdao coast in the same year and a higher divergence among samples in different years.The results showed the efficiency of 2b-RAD in the exploration of SNPs in U.prolifera and provided the first genome-wide scale evidence for the origin of the large-scale green tides on the Qingdao coast.This study improved our understanding of the reproductive strategy and genetic diversity of the green tide causative species and will help further reveal the biological causes of the green tide in China.展开更多
The tide plays a pivotal role in the ocean,affecting the global ocean circulation and supplying the bulk of the energy for the global meridional overturning circulation.To further investigate internal tides and their ...The tide plays a pivotal role in the ocean,affecting the global ocean circulation and supplying the bulk of the energy for the global meridional overturning circulation.To further investigate internal tides and their impacts on circulation,it is imperative to incorporate tidal forcing into the eddy-resolving global ocean circulation model.In this study,we successfully incorporated explicit tides(eight major constituents)into a global eddy-resolving general ocean circulation model and evaluated its tidal simulation ability.We obtained harmonic constants by analyzing sea surface height through tidal harmonic analysis and compared them with the analysis data Topex Poseidon Cross-Overs v9(TPXO9),the open ocean tide dataset from 102 open-ocean tide observations,and tide gauge stations from World Ocean Circulation Experiment.The results demonstrated that the State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics/Institute of Atmospheric Physics(LASG/IAP)Climate System Ocean Model 3.0(LICOM3.0)effectively simulated tides,with errors predominantly occurring in nearshore regions.The tidal amplitude simulated in LICOM3.0 was greater than that of TPXO9,and these high-amplitude areas exhibited greater errors.The amplitude error of the M_(2) constituent was larger,while the phase error of the K_(1) constituent was more significant.Furthermore,we further compared our results with those from other models.展开更多
In 2022,Yellow Sea green tide caused by Ulva prolifera reached a historic minimum and the coastal areas of Shandong Peninsula were less affected.However,the largest amount of seaweed biomass has been washed ashore on ...In 2022,Yellow Sea green tide caused by Ulva prolifera reached a historic minimum and the coastal areas of Shandong Peninsula were less affected.However,the largest amount of seaweed biomass has been washed ashore on Lianyungang and Rizhao coasts since 2015.We studied the development pattern of Yellow Sea green tide in 2022,and analyzed the key environmental factors on the growth and drifting,then discussed the possible reasons that resulted in the massive stranding of green tide biomass in Lianyungang and Rizhao.Results show under the combined influence of the east to southeast winds and currents with shoreward anomalies,green tide drifted to the coastal waters between Shandong and Jiangsu provinces and the distribution areas located westward compared with previous years(2008–2021).Floating U.prolifera rafts from the coastal waters of Binhai and Sheyang drifted continuously into the coastal waters of Lianyungang and Rizhao,providing important supplements for Yellow Sea green tide.Because green tide in 2022 distributed close to the coastal waters,the abundant nutrients might support their continuous high growth rate.In addition,the amount of rainfall around Shandong Peninsula from late June to early July were significantly higher than in previous years,which might promote the development of green tide to some extent.展开更多
文摘目的验证TIDE(Track of Indels by Decomposition)和TIDER(Tracking of Insertion,DEletions and Recombination events)分析绵羊囊胚基因组编辑结果的可靠性,为检测基因组编辑绵羊胚胎突变效率提供精准、及时的分析方法。方法TIDE是1种简便精准的检测方法,能精确测定CRISPR/Cas9在细胞群体中引发的靶向突变谱型和频率。利用TIDE平台对绵羊囊胚的基因组诱导突变进行表征分析与量化。结果以MSTN基因突变绵羊的基因组样本为材料,进行PCR扩增并构建T载体,随机选取单克隆测序。测序结果证实了TIDE分析基因组编辑结果的准确性。通过将TIDER分析单链寡核苷酸模板整合产生的编辑结果与DNA限制性酶切图谱对比,验证了TIDER分析外源DNA短片段导入的准确性。结论TIDE和TIDER工具可有效量化绵羊囊胚突变率,识别主要的插入和缺失类型,是高效经济的基因突变分析方法。
基金supported by the National Natural Science Foundation of China (Grant Nos. 42125402 and 42174183)the National Key Technologies R&D Program of China (Grant No.2022YFF0503703)+2 种基金the B-type Strategic Priority Program of the Chinese Academy of Sciences (Grant No. XDB41000000)the foundation of the National Key Laboratory of Electromagnetic Environment and the Fundamental Research Funds for the Central Universitiesthe Chinese Meridian Project
文摘Accurate knowledge of mesospheric winds and waves is essential for studying the dynamics and climate in the mesosphere and lower thermosphere(MLT)region.In this study,we conduct a comparative analysis of the mesosphere tidal results obtained from two adjacent meteor radars at low latitudes in Kunming,China,from November 2013 to December 2014.These two radars operate at different frequencies of 37.5 MHz and 53.1 MHz,respectively.However,overall good agreement is observed between the two radars in terms of horizontal winds and tide observations.The results show that the dominant tidal waves of the zonal and meridional winds are diurnal and semidiurnal tides.Moreover,we conduct an exhaustive statistical analysis to compare the tidal amplitudes and vertical wavelengths recorded by the dual radar systems,which reveals a high degree of alignment in tidal dynamics.The investigation includes variances and covariances of tidal amplitudes,which demonstrate remarkable consistency across measurements from both radars.This finding highlights clear uniformity in the mesospheric tidal patterns observed at low latitudes by the two neighboring meteor radars.Results of the comparative analysis specifically underscore the significant correlation in vertical wavelength measurements,validating the robustness of radar observations for tidal research.
基金The Doctor Foundation of Hebei Normal University of Science and Technology under contract No.2024YB039the Fundamental Research Funds for the Provincial Universities of Hebei under contract Nos 2023JK07 and 2022JK03+3 种基金the Major Science and Technology Support Project of Hebei Province under contract No.242S3301Zthe Fujian Provincial Natural Science Foundation of China under contract No.2021J05172the National Natural Science Foundation of China under contract No.42176244the CAS Key Deployment Project of Centre for Ocean Mega-Research of Science under contract No.COMS2020Q07.
文摘We use a high-resolution numerical simulation to analyze the generation and evolution of semidiurnal internal tides in the Weddell-Scotia Confluence.Our results indicate that two ridges near the Antarctic Peninsula,the South Scotia Ridge(SSR)and the Philip Ridge(PR),are energetic sources of semidiurnal internal tides.The strongest semidiurnal barotropic to baroclinic energy conversion occurs around the crest of the SSR,reaching 0.3 W/m^(2).The depthintegrated,tidally averaged semidiurnal internal tide energy fluxes that radiated from the SSR reach about 2 kW/m.The northward semidiurnal internal tide energy fluxes entering the Scotia Sea are stronger than the southward energy fluxes entering the Powell Basin.For the SSR region between the South Orkney Plateau and Elephant Island,the areaintegrated barotropic to baroclinic conversion rate is 0.71 GW,of which 0.56 GW(79%)dissipates locally.The dissipation of internal tides occurs mainly in water depths of less than 1000 m.The dissipation rate over the SSR is as high as 10−7 W/kg.The energy fluxes create an anticlockwise gyre between the SSR and PR.The horizontal kinetic energy and available potential energy oscillate sinusoidally with peaks occurring at quarter-wavelength intervals.This energy pattern suggests the formation of standing waves due to interference between the semidiurnal internal tides originating from the SSR and PR.The results of this study show that internal tides and related mixing need to be considered in the analysis of water mass transformation in the Southern Ocean.
基金The National Natural Science Foundation of China under contract No.42406188the Natural Science Foundation of Liaoning Province under contract No.2024-BS-022+1 种基金the Dalian High-Level Talent Innovation Program under contract No.2022RG02the Fundamental Research Funds for the Central Universities under contract No.3132025107.
文摘Red tide is an ecological disaster caused by the excessive proliferation of photosynthetic algae in the ocean.The frequent occurrences of red tide have brought serious harms to the marine aquaculture and caused significant economic losses to the marine industry.Red tide prediction can alleviate and even stop the long-term damages to marine ecosystems,which helps maintain the ecological balance of the ocean environment and contributes to the Sustainable Development Goal of“life below water”formulated by the United Nations.Aiming at red tide prediction using remote sensing technology,this study proposed a novel approach of red tide prediction using time-series hyperspectral observations,and examined the proposed method in the Xinghai Bay,China.Three spectral indices,namely the twoband ratio(TBR),the three-band spectral index(TBSI),and the fluorescence baseline height(FLH),were used to reduce the dimensionality of hyperspectral data and extract spectral features.Two machine learning models including the random forest(RF)and the support vector machine(SVM)were employed to predict whether red tide would occur on a target day based on the time-series spectral indices obtained in the previous days.By comparing and analyzing the prediction results of multiple machine learning models trained with different spectral indices and temporal lengths,it is found that both the RF and the SVM models can predict the red tide outbreaks at the accuracies over 0.9 using adequate temporal lengths of input data.When the temporal length of input data is limited,however,it is suggested to use the RF model,which accurately predicts red tide outbreaks using the temporal input of the 2-d TBSI.The proposed method is expected to provide oceanic and maritime agencies with early warnings on red tide outbreaks and ensure the safety of the coastal environment in large spatial scales using optical remote sensing technology.
基金supported by the National Natural Science Foundation of China(No.41876015)。
文摘Ocean mixing is a consequence of essential dynamic processes such as internal tides and lee waves that occur near the seafloor topography.Internal tides and lee waves are generated by barotropic tidal currents and geostrophic flows,respectively.Ocean current is composed of multiple flows;thus,internal tides and lee waves occur concurrently in the real ocean.In this paper,the Massachusetts Institute of Technology general circulation model(MITgcm)is used to conduct 2D numerical experiments.By varying background flow intensities,the energy and dissipation relationship between internal tides and lee waves are investigated.The results reveal that the internal tide beams become asymmetric due to the influence of Doppler shift.The lee wave structure gradually leads the wave field when the background flow velocity rises constantly.The presence of a background flow increases the energy portion of the high-mode wave by up to 15%-20%.Moreover,strong shear,owing to the background flow,considerably increases dissipation.When the background flow velocity is higher than the barotropic tidal current velocity,the isopycnal overturn triggered by the lee wave generates a dissipation of the same order of magnitude as the shear.
基金Supported by the National Key R&D Program of China(No.2023YFC3108300)the Dalian High-level Talent Innovation Program(No.2022RG02)the Fundamental Research Funds for the Central Universities(No.3132023507)。
文摘As a frequently occurred marine pollution phenomenon,red tides of water body due to eutrophication cause massive mortality of marine organisms and serious ecological problems.The early warning and prediction of red tide outbreak can provide guidance to the coastal management,and is of great value to the aquaculture industry and marine environment protection.An approach for the risk assessment of red tide occurrence using spectral indices was made.The optimal spectral indices were explored from three candidates,namely two-band ratio(TBR)method,three-band spectral index(TBSI)method,and fluorescence baseline(FLB)method.The correlations between the spectral indices and the red tide occurrence were quantitatively evaluated through analysis of variance(ANOVA).The risk maps for the Beibu Gulf and the Bohai Bay in China were produced with the normalized spectral indices based on the multi-spectral observation from Sentinel-3 satellite.Results show that both TBR and TBSI values have significant correlations with the occurrences of red tide as the ANOVA results.TBSI illustrated correctly the risk of red tide occurrence in the risk maps and was the optimal spectral index offshore risk assessment of red tide.FLB method failed to recognize the high-risk regions and may not be the appropriate spectral index.The risk assessment method proposed in this study can provide early alarms on red tide occurrence and help timely the countermeasure against potential harms.
基金supported by the National Key Research and Development Program of China(No.2022YFC2807500)Laoshan Laboratory(No.LSKJ202203201)+1 种基金the National Natural Science Foundation of China(Nos.42206147,42120104006 and 42176111)the Natural Science Foundation of Shandong Province(Nos.ZR2022QD046,ZR2021QD051).
文摘Ulva prolifera green tides are becoming aworldwide environmental problem,especially in the Yellow Sea,China.However,the effects of the occurrence of U.prolifera green tides on the community organization and stability of surrounding microbiomes have still not been de-termined.Here,the prokaryotic microbial community network stability and assembly char-acteristics were systematically analyzed and compared between the green tide and non-green tide periods.U.prolifera blooms weaken the community complexity and robustness of surrounding microbiomes,increasing fragmentation and decreasing diversity.Bacteria and archaea exhibited distinct community distributions and assembly patterns under the influ-ence of green tides,and bacterial communities were more sensitive to outbreaks of green tides.The bacterial communities exhibited a greater niche breadth and a lower phyloge-netic distance during the occurrence of U.prolifera green tides compared to those during the non-green tide period while archaeal communities remained unchanged,suggesting that the bacterial communities underwent stronger homogeneous selection and more sensitive to green tide blooms than the archaeal communities.Piecewise structural equation model analysis revealed that the different responses of major prokaryotic microbial groups,such as Cyanobacteria,to environmental variables during green tides,were influenced by the variations in pH and nitrate during green tides and correlated with the salinity gradient during the non-green tide period.This study elucidates the response of the adaptability,associations,and stability of surrounding microbiomes to outbreaks of U.prolifera green tides.
基金supported by the open funding of the Technology Innovation Center for South China Sea Re-mote Sensing,Surveying and Mapping Collaborative Ap-plication,Ministry of Natural Resources,P.R.China(No.RSSMCA-2024-B001)the National Natural Science Foundation of China(Nos.T2261149752 and 42476172).
文摘Motivated by the wide applications of empirical global ocean tide models in Earth science,particularly in shallow waters and coastal regions,we proposed an updated global ocean tide model representing all major diurnal and semidiurnal tidal constituents.We integrated the development technique of the Technical University of Denmark(DTU10)model and calculated the residual tides using the intermediary FES2012 model.We utilized all available Topex/Poseidon,Jason-1,Ocean Surface Topography Mission(OSTM)/Jason-2 primary and tandem missions to develop the new model.To reduce the discrepancies between the model and in situ measurements estimated S2 tide constituent,the ERA-Interim model was selected for dry tropospheric correction of altimetry data.The newly developed model with an improved spatial resolution from 1/8°×1/8°to 1/16°×1/16°was assessed and compared against contemporary global ocean tide models using in situ measurements in coastal regions,continental shelf waters,marginal seas,and deep waters.The results demonstrate improvements in shallow waters and coastal regions,particularly in representative coastal re-gions such as the Northwest European Shelf and East Asian Marginal Seas.Moreover,the model performs well in simulating S_(2) and K_(2) tides with root mean square differences of 0.355 and 0.234 cm,respectively,against in situ measurements in deep waters world-wide.Compared with that of DTU10,the root sum square of the new model for the eight main tidal constituents decrease by 8.4%to 0.997 cm.
文摘This study on the distribution of phosphate and its relation to phytoplankton biomass in Western Xiamen Harbor using marine ecosystem enclosures to isolate the culture water from the tidal currents and salinity changes outside indicated that the phytoplankton biomass variation closely related to dissolved inorganic phosphorus (DIP) in the seawater as described by the equation: [Chl a]=A×e -B[PO4] . The biomass changes lagged by about two days the corresponding DIP. The research also dealt with the minimal DIP concentration for stopping diatom bloom and the possible maximal diatom biomass was estimated from the DIP external concentration in the seawater. The threshold of DIP initiating Skeletonema costatum red tide was calculated for use as an index to forecast its red tides. In addition, the relationships between a dinoflagellate red tide and nutrients are discussed. The results showed that the multiplication of dinoflagellate was not entirely dependent on the nutrients in the seawater.
文摘A series of enclosed ecosystem experiments were conducted in a land based tank near the seaside of West Xiamen Harbor.The results of experiments conducted in different seasons and years showed a repeatble phytoplankton succession. In this relatively stable ecosystem with added nutrients and trace metals,diatoms dominated initially,dinoflagellates dominated in the later stage,and dinoflagellate red tides eventually occurred.Vitamin B 12 enrichment may speed up this succession process. Stirring the water column could stop this process. Soluble Mn at a level of 3-4 μg/L in seawater,which also is the existing concentration of solu ble Mn in Xiamen Harbor seawater,is sufficient for the multiplication of algae and occurrence of red tide.The present study showed that excessive soluble Mn in Xiamen Harbor cannot cause red tide,and that Fe was one of the important factors causing diatom red tide in this present study..
文摘To study the Taiwan Strait （TS）, an unusual sea area, the numerical model in marginal seas of China is used to simulate and analyze the tidal wave motion in the strait. The numerical modeling experiments reproduce the amphidromic system of the M2 tide in the south end of the Taiwan strait, and consequently confirm the existence of the degenerate amphidromic system. On this basis, further discussion is conducted on the M2 system and its formation mechanism. It can be concluded that the tidal waves of the TS is consisted of the progressing wave from the north entrance and the degenerate amphidromic system from the south entrance, in which the progressing wave from the north entrance dominates the tidal wave motion in the strait. Except for the convergent effect caused by the landform and boundary, the degenerate amphidromic system produced in the south of the strait is another important factor for the following phenomena： the large tidal range in the middle of the strait, the concentrative zone of co-amplitude and co-phase line in the south of the strait. The degenerate amphidromic system is mainly produced by the incident Pacific Ocean tidal wave from the Luzon strait and the action by the shoreline and landform. The position of the amphidromic point is compelled to move toward southwest until degenerating by the powerful progressing wave from the north entrance.
文摘In the numerical studies of a real tide M4 resonance system, the Xiangshan Port which is a partially-closed bay, Dong et al. [1999. Acta Oceanologica Sinica, 21 （3）： 1-6] found the interesting phenomenon that the advection plays an important role in inhibiting the growth of the amplitude of the tidal second-order resonance response （M4）. This result is contrary to the general traditional ideas for a non-resonance system. How this phenomenon is interpreted and what internal mechanism is behind the phenomenon are the main focuses of this study. The followings are examined： （1） the dynamic features of a second-order resonance system of tide; （2） the dominating factors on the second-order resonance responses; （3） the effects of both the friction and the advection on the second-order resonance responses; and （4） their roles in dominating the second-order resonance response and internal mechanisms by using the analytical methods. The respective results show that： （1） Both the bottom friction and the advection play significant roles in dominating the magnitude of the amplitude of the second-order resonance responses; （2） the effect of the friction on the second-order resonance response depends on the distribution ratio of the work-done of the system to friction force exhausted into between the damping of the first-order system and the inner excitation of the second-order system; （3） the advection plays a positive role in increasing the amplitude of the second-order non-resonance response in the second order non-resonance of tide; （4） in a second-order resonance system of tide, the effect of the advection may be either to increase or to decrease the amplitudes of the second-order resonance responses of tide, which depends on the distribution ratio mentioned above.
基金Supported by the China Postdoctoral Science Foundation(No.2013M531648)the Natural Science Foundation of Shandong Province(No.ZR2014DP008)+2 种基金the Fundamental Research Funds for the Central Universities(No.201513046)the National Special Research Fund for Non-Profit Marine Sector(No.201305009)the Joint Funds of the National Natural Science Foundation of China(No.U1406403)
文摘Green tides have occurred every year from 2007 to 2014 in the Yellow Sea. Ulva prolifera （Mtiller） J. Agardh has been identified as the bloom-forming alga, co-occurring with U. intestinalis. We observed distinct strategies for both algal species during green tides. U.prolifera exhibited a high abundance initially and then decreased dramatically, while U. intestinalis persisted throughout. The antioxidant system responses of these two macroalgae were compared in the late phase of a green tide （in-situ） and after laboratory acclimation. Lipid peroxidation and antioxidant system responses differed significantly between the two. Malondialdehyde and hydrogen peroxide contents increased significantly in-situ in U. prolifera, but not in U. intestinalis. In U. prolifera, we observed a significant decrease in total antioxidant ability （T-AOC）, antioxidant enzymes （SOD and Apx）, and non-enzyme antioxidants （GSH and AsA） in-situ. U. intestinalis showed the same pattern of T-AOC and SOD, but its Gpx, Apx, and GSH responses did not differ significantly. The results suggest that U. prolifera was more susceptible than U. intestinalis to the harsh environmental changes during the late phase of a Yellow Sea green tide. The boom and bust strategy exhibited by U. prolifera and the persistence of U. intestinalis can be explained by differences in enzyme activity and antioxidant systems.
基金the Brazilian agencies FAPESP (Sao Paulo State Research Agency) and CNPq (National Council for Scientific and Technological Development) for funding throughout the development of this work
文摘The Brazilian coast is characterized by different tidal regimes and distinct meteorological influences. The northern part has larger tidal amplitudes and is permanently affected by trade winds and tropical disturbances; the southern portion has smaller tidal amplitudes and is frequently influenced by extratropical cyclone activity. Besides these aspects, many features regarding current structure and behavior are also present, such as the equatorial system of currents, the subtropical gyre and the corresponding western boundary currents, and the Brazil-Malvinas confluence region. Within this context, efforts were made to develop the BRAZCOAST system, capable of describing the processes that determine the oceanic circulation from large to coastal scales. A customized version of the Princeton Ocean Model(POM) was implemented in a basin-scale domain covering the whole of the tropical and southern Atlantic Ocean, with 0.5° spatial resolution, as well as three nested grids with(1/12)° resolution covering the different parts of the Brazilian shelf, in a one-way procedure. POM was modified to include tidal potential generator terms and a partially-clamped boundary condition for tidal elevations. The coarse grid captured large-scale features, while the nested grids detailed local circulations affected by bathymetry and coastal restrictions. An interesting aspect at the coarse grid level was the relevance of the Weddell Sea to the location of the tidal amphidromic systems.
文摘This study was performed to compare storm surges/tide simulated by the regional and coastal storm surges/tide forecast system （RTSM （regional tide/storm surges model）, CTSM （coastal tide/storm surges model）） using two different inputs from weather models （RDAPS （Regional Data Assimilation and Prediction System） and KWRF （Korea Weather and Research Forecasting）） during two typhoons that occurred between 2007 and 2008. Both the RDAPS and KWRF are the operational weather forecasting system in KMA （Korea Meteorological Administration）. The horizontal resolutions of RDAPS and KWRF are 30 and 10 km, respectively. The storm surges/tide was hind casted using sea wind and pressure fields of two Typhoons which was approaching Korean Peninsula. The CTSM using input from KWRF simulate very well the storm surges/tide pattern in the complex coastal areas. The result showed that the storm surges by the coastal storm surges/tide model with high resolution input was in well agreement with the observed sea level occurred by high tide and storm surges in the coastal areas.
基金supported by the Joint Fund between NSFC and Shandong Province(No.U1906210)the China National Key Research and Development Program(No.2016YFC1402101).
文摘Since 2007,the large-scale green tide caused by Ulva prolifera(U.prolifera)have occurred as a recurrent phenomenon in the southern Yellow Sea of China.Field surveys and satellite remote sensing showed that the small scattered patches of green tide algae were first observed along the Porphyra agriculture area of the Subei Shoal in late April.In this study,we attempted to identify the role of eutrophication in the origin of the green tide in the Subei Shoal and its adjacent area.Subei Shoal and its adjacent area are characterized by rich nutrients,especially NO_(3)^(-)-N,NH_(4)^(+)-N,PO_(4)^(3-)-P,and other bioavailable components(such as urea-N and amino acids).In the spring of 2017,the average concentrations of NO_(3)^(-)-N were 19.01±11.01μmolL^(-1),accounting for 86.68%of the dis-solved inorganic nitrogen(DIN).In addition,the average concentration of NH4^(+)-N was 2.51±1.60μmolL^(-1).PO_(4)^(3-)-P had an average concentration of 0.14±0.13μmolL-1.The average concentrations of urea-N and total hydrolyzed amino acids(THAA)were 1.73±1.36μmolL^(-1)and 1.33±0.80μmolL^(-1),respectively.Rich nutritive substances play a key role in the rapid production of U.prolifera and make the Jiangsu coastal water an incubator for green tide.
基金Supported by the Laoshan Laboratory (No.LSKJ202204005)the Mount Tai Scholar Climbing Plan to Song SUNthe Open Fund of CAS Key Laboratory of Marine Ecology and Environmental Sciences,Institute of Oceanology,Chinese Academy of Sciences (No.KLMEES201801)
文摘Ulva prolifera is the causative species of the annually occurring large-scale green tides in China since 2007.Its specific biological features on reproductivity strategies,as well as intra-species genetic diversity,are still largely unknown,especially at the genome level,despite their importance in understanding the formation and outbreak of massive green tides.In the present study,the restriction site-associated DNA genotyping approach(2b-RAD)was adopted to identify the genome-wide single-nucleotide polymorphisms(SNPs)of 54 individual thalli including samples collected from Subei Shoal in 2019 and Qingdao coast from 2019 to 2021.SNPs genotype results revealed that most of the thalli in 2019 and 2020 were haploid gametophytes,while only half of the thalli were gametophytes in 2021,indicating flexibility in the reproductive strategies for the formation of the green tides among different years and the dominance of asexual and vegetative reproductive mode for the floating period.Besides,population analysis was conducted,and it revealed a very low genetic diversity among samples from Subei Shoal and the Qingdao coast in the same year and a higher divergence among samples in different years.The results showed the efficiency of 2b-RAD in the exploration of SNPs in U.prolifera and provided the first genome-wide scale evidence for the origin of the large-scale green tides on the Qingdao coast.This study improved our understanding of the reproductive strategy and genetic diversity of the green tide causative species and will help further reveal the biological causes of the green tide in China.
基金The National Natural Science Foundation of China under contract Nos 41931182,42090040,42176024,and 42206006the National Key Program for Developing Basic Sciences under contract No.2022YFC3104802.
文摘The tide plays a pivotal role in the ocean,affecting the global ocean circulation and supplying the bulk of the energy for the global meridional overturning circulation.To further investigate internal tides and their impacts on circulation,it is imperative to incorporate tidal forcing into the eddy-resolving global ocean circulation model.In this study,we successfully incorporated explicit tides(eight major constituents)into a global eddy-resolving general ocean circulation model and evaluated its tidal simulation ability.We obtained harmonic constants by analyzing sea surface height through tidal harmonic analysis and compared them with the analysis data Topex Poseidon Cross-Overs v9(TPXO9),the open ocean tide dataset from 102 open-ocean tide observations,and tide gauge stations from World Ocean Circulation Experiment.The results demonstrated that the State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics/Institute of Atmospheric Physics(LASG/IAP)Climate System Ocean Model 3.0(LICOM3.0)effectively simulated tides,with errors predominantly occurring in nearshore regions.The tidal amplitude simulated in LICOM3.0 was greater than that of TPXO9,and these high-amplitude areas exhibited greater errors.The amplitude error of the M_(2) constituent was larger,while the phase error of the K_(1) constituent was more significant.Furthermore,we further compared our results with those from other models.
基金Supported by the National Key R&D Program of China(No.2022YFC3106005)the Shandong Provincial Natural Science Foundation(No.ZR2021MD122)+1 种基金the MNR Key Laboratory of Eco-Environmental Science and Technology,China(No.MEEST-2023-04)the Shandong Provincial Key Laboratory of Marine Ecological Environment and Disaster Prevention and Mitigation(No.201708)。
文摘In 2022,Yellow Sea green tide caused by Ulva prolifera reached a historic minimum and the coastal areas of Shandong Peninsula were less affected.However,the largest amount of seaweed biomass has been washed ashore on Lianyungang and Rizhao coasts since 2015.We studied the development pattern of Yellow Sea green tide in 2022,and analyzed the key environmental factors on the growth and drifting,then discussed the possible reasons that resulted in the massive stranding of green tide biomass in Lianyungang and Rizhao.Results show under the combined influence of the east to southeast winds and currents with shoreward anomalies,green tide drifted to the coastal waters between Shandong and Jiangsu provinces and the distribution areas located westward compared with previous years(2008–2021).Floating U.prolifera rafts from the coastal waters of Binhai and Sheyang drifted continuously into the coastal waters of Lianyungang and Rizhao,providing important supplements for Yellow Sea green tide.Because green tide in 2022 distributed close to the coastal waters,the abundant nutrients might support their continuous high growth rate.In addition,the amount of rainfall around Shandong Peninsula from late June to early July were significantly higher than in previous years,which might promote the development of green tide to some extent.
