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The SCO Tianjin Summit Empowers the SCO’s High-Quality Development and Global Governance Reform
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作者 Chen Yurong 《Contemporary World》 2025年第5期8-14,共7页
From 31 August to 1 September 2025,the 25th Meeting of the Council of Heads of State of the Shanghai Cooperation Organization(the“Tianjin Summit”)and the“Shanghai Cooperation Organization Plus”Meeting were success... From 31 August to 1 September 2025,the 25th Meeting of the Council of Heads of State of the Shanghai Cooperation Organization(the“Tianjin Summit”)and the“Shanghai Cooperation Organization Plus”Meeting were successfully held in Tianjin,China.This was the fifth SCO summit hosted by China and the largest summit since the organization’s founding 24 years ago.The Summit produced fruitful outcomes that injected powerful momentum into the SCO’s future development and signaled that the SCO has entered a new stage of high-quality development. 展开更多
关键词 global governance reform high quality development shanghai cooperation organization tianjin Shanghai Cooperation Organization tianjin Summit
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Assessment of Carbon Sink Capacity in Seawater Suspension Cage Aquaculture of Shellfish in Tianjin from 2015 to 2022
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作者 Zhiru FU Xin QI +4 位作者 Jian LI Haonan CHEN Chao MA Lijun SHI Liwei SHI 《Asian Agricultural Research》 2025年第2期30-33,共4页
[Objectives]To assess the carbon sink capacity in seawater suspension cage aquaculture of shellfish in Tianjin from 2015 to 2022.[Methods]The carbon sink capacity of different shellfish species was evaluated using bot... [Objectives]To assess the carbon sink capacity in seawater suspension cage aquaculture of shellfish in Tianjin from 2015 to 2022.[Methods]The carbon sink capacity of different shellfish species was evaluated using both physical and value assessment methods.[Results]The shellfish cultivated in seawater suspension cages in Tianjin exhibited a significant capacity for carbon sinks.The amounts of carbon removed by suspension cage aquaculture of Rapana venosa,Crassostrea gigas,Scapharca subcrenata,Scapharca broughtonii and Argopectens irradias were 448.297,403.398,89.463,40.657,and 106.719 t,respectively.Furthermore,the total volume of shellfish cultivated in seawater exhibited a consistent upward trend over time,correlating with an annual increase in the amount of carbon removed.Among the shellfish cultivated in seawater suspension cages,the order of carbon sink capacity was as follows:C.gigas>R.venosa>A.irradias>S.subcrenata and S.broughtonii.In terms of the carbon sink capacity of soft tissues,the ranking was as follows:A.irradias>R.venosa>C.gigas>S.subcrenata and S.broughtonii.The structural and yield factors associated with seawater suspension cage aquaculture of shellfish significantly influenced the enhancement of the total carbon sink of cultivated shellfish.Notably,structural factors had a greater impact on the increase in the carbon sink of cultivated shellfish compared to total yield factors.[Conclusions]The findings will serve as a reference for enhancing the carbon sink potential of fisheries and achieving sustainable development in seawater aquaculture in Tianjin. 展开更多
关键词 Seawater suspension cage aquaculture SHELLFISH Carbon sink ASSESSMENT tianjin
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The Role of Village Regulations in Litigation Source Governance:A Legal and Empirical Analysis Based on Tianjin’s 2023 Model Village Regulations
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作者 Siying Huo Yuefeng Wang +1 位作者 Ziyuan Xu Guo Yang 《Proceedings of Business and Economic Studies》 2025年第4期446-454,共9页
This study examines the role of village regulations within China’s Litigation Source Governance(LSG)framework,specifically analyzing Tianjin Municipality’s 2023 Model Village Regulations.Employing legal analysis and... This study examines the role of village regulations within China’s Litigation Source Governance(LSG)framework,specifically analyzing Tianjin Municipality’s 2023 Model Village Regulations.Employing legal analysis and Natural Language Processing(NLP)techniques,the research evaluates the effectiveness,enforceability,and thematic orientation of these regulations in grassroots dispute resolution.Findings reveal a pronounced reliance on moral governance provisions,limited judicial recognition,and significant implementation challenges due to the predominance of non-binding(soft)clauses.The study recommends enhancing judicial recognition through formal confirmation mechanisms,increasing legally binding clauses,and integrating village-level governance more closely with formal judicial processes.This approach not only strengthens local governance but also provides valuable insights for nationwide replication,supporting broader goals of rural stability and governance modernization. 展开更多
关键词 Village regulations Litigation source governance Judicial recognition Natural language processing Grassroots governance tianjin municipality Legal enforceability
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2025 Gaoyang Textile&Apparel Event:Boosting supply chain&industry upgrade in Beijing–Tianjin–Hebei region
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作者 Qiu Shuchen 《China Textile》 2025年第3期32-33,共2页
On May 21st,the launch ceremony of the 2025 Textile and Clothing"Optimization of Supply and Upgrade"event was held in Gaoyang,Hebei Province.The theme of the event is to promote the coordinated development a... On May 21st,the launch ceremony of the 2025 Textile and Clothing"Optimization of Supply and Upgrade"event was held in Gaoyang,Hebei Province.The theme of the event is to promote the coordinated development and supply-demand docking of the textile and clothing industry in the Beijing–Tianjin–Hebei Region.Representatives from the textile and clothing industries gathered in Gaoyang to discuss the coordinated development of regional industries.The Ministry of Industry and Information Technology,Industry Associations,and leaders of the three local governments jointly announced the official launch of the national industrial upgrading action. 展开更多
关键词 beijing tianjin hebei region industrial upgrading action industry upgrade supply chain TEXTILE coordinated development clothing supply demand docking
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Application of COI gene in the classification of the gobioid fishes in the Tianjin coastal area
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作者 FANG Enjun ZENG Xiangxi +4 位作者 WANG Hong ZHANG Xue ZHANG Jingwei GAO Yan WANG Yu 《Marine Science Bulletin》 2025年第1期28-37,共10页
In order to clarify the mitochondrial bar code sequence and taxonomic information of common goby fish in Tianjin coastal marine areas,seven species of goby fish were collected from Tianjin coastal marine areas,identif... In order to clarify the mitochondrial bar code sequence and taxonomic information of common goby fish in Tianjin coastal marine areas,seven species of goby fish were collected from Tianjin coastal marine areas,identified by morphology,and DNA was extracted for amplification and sequencing of mitochondrial COI gene fragments.Phylogenetic analysis and molecular identification software MEGA were used to analyze the sequence characteristics and molecular classification of COI gene fragments of 12 common goby species in Tianjin coastal marine areas.Seven COI gene fragments of Goby were amplified and sequenced,the average GC content was lower than AT content,the base had a bias in the codon position distribution,and the highest GC content was the second base,which was similar to the COI gene base of many fishes.The codon base variation rate was 19.4%,and the ratio of transitions to transversions was 1.3.Phylogenetic distance analysis and phylogenetic tree analysis clarified the phylotaxonomic and evolutionary relationships of 12 major goby species in Tianjin coastal marine areas.Genetic distance indicates that Amblychaeturichthys hexanema and Synechogobius ommaturus are the same species.The high codon variation rate of codon encoding gene in Goby may be related to its good environmental adaptability. 展开更多
关键词 tianjin coastal marine areas goby fish cytochrome oxidase subunit I(COI)gene phylogenetic analysis
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Estimation of Soil Organic Carbon Stocks Utilizing Machine Learning Algorithms and Multi-source Geospatial Data in Coastal Wetlands of Tianjin and Hebei,China
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作者 YANG Rui LIU Mingyue +10 位作者 ZHANG Yongbin MAN Weidong TONG Jingfen LIU Dong ZHANG Qingwen KOU Caiyao LI Xiang LIU Yahui TIAN Di YIN Xuan HE Jiannan 《Chinese Geographical Science》 2025年第4期707-721,I0003,共16页
Coastal wetlands are crucial for the‘blue carbon sink’,significantly contributing to regulating climate change.This study util-ized 160 soil samples,35 remote sensing features,and 5 geo-climatic data to accurately e... Coastal wetlands are crucial for the‘blue carbon sink’,significantly contributing to regulating climate change.This study util-ized 160 soil samples,35 remote sensing features,and 5 geo-climatic data to accurately estimate the soil organic carbon stocks(SOCS)in the coastal wetlands of Tianjin and Hebei,China.To reduce data redundancy,simplify model complexity,and improve model inter-pretability,Pearson correlation analysis(PsCA),Boruta,and recursive feature elimination(RFE)were employed to optimize features.Combined with the optimized features,the soil organic carbon density(SOCD)prediction model was constructed by using multivariate adaptive regression splines(MARS),extreme gradient boosting(XGBoost),and random forest(RF)algorithms and applied to predict the spatial distribution of SOCD and estimate the SOCS of different wetland types in 2020.The results show that:1)different feature combinations have a significant influence on the model performance.Better prediction performance was attained by building a model using RFE-based feature combinations.RF has the best prediction accuracy(R^(2)=0.587,RMSE=0.798 kg/m^(2),MAE=0.660 kg/m^(2)).2)Optical features are more important than radar and geo-climatic features in the MARS,XGBoost,and RF algorithms.3)The size of SOCS is related to SOCD and the area of each wetland type,aquaculture pond has the highest SOCS,followed by marsh,salt pan,mud-flat,and sand shore. 展开更多
关键词 soil organic carbon stocks(SOCS) soil organic carbon density(SOCD) multivariate adaptive regression spline(MARS) extreme gradient boosting(XGBoost) random forest(RF) residual kriging(RK) feature optimization coastal wetlands tianjin and Hebei China
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新分离的副粘病毒Tianjin株的全基因组序列分析 被引量:5
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作者 李梅 石立莹 +3 位作者 袁立军 李晓眠 王卿 王文秀 《病毒学报》 CAS CSCD 北大核心 2008年第1期1-6,共6页
副粘病毒Tianjin株是一株对普通棉耳狨猴具有高致病性,并可能与人类下呼吸道感染密切相关的毒株。为了明确其基因结构、变异特点及种系进化地位,采用RT-PCR、测序和拼接,获得了副粘病毒Tianjin株全基因组序列,与GenBank登录的副粘病毒科... 副粘病毒Tianjin株是一株对普通棉耳狨猴具有高致病性,并可能与人类下呼吸道感染密切相关的毒株。为了明确其基因结构、变异特点及种系进化地位,采用RT-PCR、测序和拼接,获得了副粘病毒Tianjin株全基因组序列,与GenBank登录的副粘病毒科7个属和尚未分类的28株病毒及7株仙台病毒代表株,进行同源性比较及系统进化分析。结果表明,副粘病Tianjin株属于副粘病毒科、副粘病毒亚科、呼吸道病毒属,与仙台病毒关系最近。基因组全长及组成规律与仙台病毒相似,只是L基因末尾A15240C变异而使L蛋白增加了一个谷氨酸残基。副粘病毒Tianjin株存在440个独特的核苷酸变异位点,导致110个氨基酸残基的改变,系统进化上构成独立的分支。副粘病毒Tianjin株在基因组序列、宿主亲嗜性和致病性等方面与已知仙台病毒存在较大的差异,可能代表仙台病毒的一个新基因型。 展开更多
关键词 副粘病毒tianjin 仙台病毒 同源性比较 系统进化分析
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Analysis on Vapour Field for the Drought Causes in Beijing,Tianjin and Hebei Districts in Recent Years 被引量:5
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作者 杨文霞 李宏宇 +1 位作者 李宗涛 房彬 《Agricultural Science & Technology》 CAS 2010年第1期117-121,共5页
The monthly variation,temporal-spatial distribution,transportation characteristics of vapour field at middle and lower atmosphere and their effects on drought in Beijing,Tianjin and Hebei districts were studied,which ... The monthly variation,temporal-spatial distribution,transportation characteristics of vapour field at middle and lower atmosphere and their effects on drought in Beijing,Tianjin and Hebei districts were studied,which have a guiding significance on predicting climate and arranging agricultural production in the district. 展开更多
关键词 BEIJING tianjin and Hebei district DROUGHT Vapour field
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副粘病毒Tianjin株NP蛋白的表达及分析 被引量:1
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作者 王卿 李梅 +2 位作者 石立莹 袁立军 王文秀 《病毒学报》 CAS CSCD 北大核心 2008年第3期234-238,共5页
1999年,本实验室从群体性暴发的急性呼吸道感染致死的普通棉耳绒猴肺组织中分离到一株副粘病毒,命名为副粘病毒Tianjin株。经研究发现该动物中心的工作人员都有此病毒抗体,且正常人群(献血员)抗体阳性率高达46%,急性呼吸道感染... 1999年,本实验室从群体性暴发的急性呼吸道感染致死的普通棉耳绒猴肺组织中分离到一株副粘病毒,命名为副粘病毒Tianjin株。经研究发现该动物中心的工作人员都有此病毒抗体,且正常人群(献血员)抗体阳性率高达46%,急性呼吸道感染的患儿抗体阳性率为19.28%,提示此病原体与人类可能有密切的关系。经血清学、形态学及基因测序研究,表明副粘病毒Tianjin株与仙台病毒(Sendai virus,SeV)同源性较高, 展开更多
关键词 副粘病毒tianjin NP基因 同源性及进化分析
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副粘病毒Tianjin株NP蛋白的表达及分析 被引量:1
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作者 王卿 李梅 +2 位作者 石立莹 袁立军 王文秀 《中国病原生物学杂志》 CSCD 2008年第4期245-250,共6页
目的分析副粘病毒Tianjin株NP蛋白与其他SeV的同源性及种系进化关系,探讨其变异及抗原性。方法克隆NP基因,对其核苷酸和氨基酸序列进行同源性及进化分析。在原核系统中分3段表达NP蛋白。结果经Western blot分析,重组蛋白NP1、NP2和NP3... 目的分析副粘病毒Tianjin株NP蛋白与其他SeV的同源性及种系进化关系,探讨其变异及抗原性。方法克隆NP基因,对其核苷酸和氨基酸序列进行同源性及进化分析。在原核系统中分3段表达NP蛋白。结果经Western blot分析,重组蛋白NP1、NP2和NP3能与副粘病毒Tianjin株抗血清特异反应,具有天然抗原性。Tianjin株与仙台病毒BB1株NP的核苷酸和氨基酸序列同源性分别为94.5%和96.2%,与其他仙台病毒的同源性,在85.1%~88.7%和92.4%~94.7%之间。Tianjin株NP蛋白共有15个独特的氨基酸变异位点和11个与BB1株共有的变异位点。Tianjin株与BB1株NP核苷酸序列的差异大于同一进化分支内的仙台病毒之间的差异。结论副粘病毒Tian-jin株属于仙台病毒新的进化分支。重组NP蛋白具有抗原性。 展开更多
关键词 副粘病毒tianjin NP基因 原核表达 同源性及进化分析
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Macrobenthos Community Structure and Its Relations With Environmental Factors in Three Reservoirs in Tianjin 被引量:1
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作者 姜巨峰 傅志茹 +4 位作者 李文雯 张韦 孟一耕 钱红 李相普 《Agricultural Science & Technology》 CAS 2014年第6期947-953,969,共8页
[Objective] The aim was to research macrobenthos community structure and its relations with environmental factors in three reservoirs. [Method] Benthic diversity and water quality of Yuqiao Reservoir, Beidagang Reserv... [Objective] The aim was to research macrobenthos community structure and its relations with environmental factors in three reservoirs. [Method] Benthic diversity and water quality of Yuqiao Reservoir, Beidagang Reservoir and Er- wangzhuang Reservoir were investigated in May, July and September 2011, respec- tively. []Result] Of all the 14 sampling sites, 22 species of macroinvertebrates were collected, totally, which included 6 species of aquatic insects, 7 species of Mollusca, 2 species of Crustacean, 7 species of Oligochaeta. The species of macroinverte- brates during May, July and September in Yuqiao reservoir were 11, 8 and 10, respectively. The dominant species were Chironomus sinicus, and Monopylephorus limosus. The average density and biomass of the macrobenthos were 132.43 ind./m2 and 50.09 g/m2. Yuqiao reservoir was mostly at status of premediate polluted, according to the Margalef index, Shannon index and Pielou index. The species of during May, July and September in Beidagang reservoir were 5 5 and 5, respectively. The dominant species were Chironomus sinicus, Penaeus vannamei. The average density and biomass of the macrobenthos were 218 ind./m2 and 2.70 g/m2. Beidagang reservoir was mostly at status of heavy polluted, accord- ing to the Margalef index, Shannon-wiener index, Pielou index. The species of during May, July and September in Erwangzhuang reservoir were 3, 5 and 5, respectively. The dominant species were Monopylephorus limosus, Chi- ronomus sinicus. The average density and biomass of the macrobenthos were 104 ind/m2 and 0.20 g/m2. Erwangzhuang reservoir was mostly at status of intermediate polluted, according to the Margalef index, Shannon index, Pielou index. The density of zoobenthos in Yuqiao Reservoir was positively significantly correlated with TP (P〉 0.05), negatively correlated with NH4+-N (P〉0.05), positively correlated with COD (P〈 0.01), not significantly correlated with other environment factors (P〉0.05). There was no significant correlation between biomass of zoobenthos and environment factors. The density of zoobenthos in Beidagang Reservoir with NH4+-N was positively signif- icantly correlated (P〈0.05), not significantly correlated with other aquatic environment factors (P〉0.05). There was no significant correlation between biomass of zoobenthos and environment factors. The density of zoobenthos in Erwangzhuang Reser- voir with NO3-N was positively significantly correlated (P〈0.05), not significant cor- related with other aquatic environment factors (F〉0.05). The biomass of zoobenthos in Erwangzhuang Reservoir with NH4+-N was positively significantly correlated (P〈 0.05), not significant correlated with other aquatic environment factors (P〉0.05). [Conclusion] It is indicated that this three reservoirs were all eutrophied and polluted, and the eurephication extent change of water in three reservoirs is as follows: Beidagang reservoir〉Erwangzhuang reservoir〉Yuqiao reservoir. 展开更多
关键词 MACROBENTHOS Community structure Diversity index Water quality as-sessment Large reservoirs tianjin
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Analysis on Landscape Pattern of Urban Green Space in Tianjin City 被引量:1
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作者 孙艳玲 郭鹏 马振兴 《Journal of Landscape Research》 2011年第1期25-27,30,共4页
Based on IKONOS satellite image in August of 2008,and by using GIS technique and spatial pattern analysis software FRAGSTATS,the paper analyzed landscape pattern of urban green space in Tianjin City from the aspects o... Based on IKONOS satellite image in August of 2008,and by using GIS technique and spatial pattern analysis software FRAGSTATS,the paper analyzed landscape pattern of urban green space in Tianjin City from the aspects of patch composition and level of category and landscape.The result showed that there were manymin-sized patches in Tianjin City while large patches occupied a large proportion of the total area of green space.Green spaces were unbalanced in distribution and unreasonable in structure.It suggested adjusting the structure of urban green space,reducing the degree of landscape fragmentation,strengthening the construction of large and mid-sized green patches,so as to improve ecological function of green space. 展开更多
关键词 URBAN green SPACE LANDSCAPE PATTERN tianjin CITY
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RS/GIS-Based Analysis of Landscape Pattern Evolution in Tianjin City 被引量:1
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作者 孙艳玲 郭鹏 《Journal of Landscape Research》 2011年第2期10-13,共4页
Taking the downtown area of Tianjin City and Beichen District,Xiqing District,Jinnan District and Dongli District in its neighborhood as the study area,TM remote sensing images(with a resolution ratio of 30 m)of 1993 ... Taking the downtown area of Tianjin City and Beichen District,Xiqing District,Jinnan District and Dongli District in its neighborhood as the study area,TM remote sensing images(with a resolution ratio of 30 m)of 1993 and 2001 were utilized to analyze landscape structures and evolution characteristics of the study area by calculating its landscape pattern indexes with the help of ArcGIS9.3.The results showed that:A.Ratios of landscape components changed greatly,area ratio of each landscape component varied in different degrees.Area of farmland decreased from 32.7% to 29.2%;area of green spaces decreased from 27.4% to 20.4%;water area decreased from 26.1% to 23.7%;construction land area increased from 13.8% to 26.8%,and replaced the dominant status of farmland.B.There were 99 366 landscape patches in the study area by 2001,which was almost twice as much as 52 832 landscape patches in 1993.C.Urbanization and increasing human interventions led to the increase of landscape diversity indexes,and the aggravated fragmentation.Through analyzing dynamic changes of urban landscape structures in Tianjin City,evolution trend of the structural characteristics of urban spaces and its factors can be better understood,city pattern and land use properly planned,benign development of economy,society and eco-environment realized. 展开更多
关键词 LANDSCAPE ECOLOGY SPATIAL PATTERN GIS tianjin
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Study on the Spatial Pattern Changes of Land Use Based on Fractal Dimensions in Tianjin New Coastal Area 被引量:3
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作者 白新萍 《Agricultural Science & Technology》 CAS 2011年第6期879-882,919,共5页
[Objective] The aim was to discuss the spatial pattern changes of land use in Tianjin new coastal area based on fractal dimensions.[Method] By dint of remote and geographic information system technology to obtain the ... [Objective] The aim was to discuss the spatial pattern changes of land use in Tianjin new coastal area based on fractal dimensions.[Method] By dint of remote and geographic information system technology to obtain the data of urban land use in new coastal area from 1993 to 2008,the boundary dimension,radius dimension and information dimension of each land use type were calculated based on fractal dimension.In addition,the revealed land use spatial dimension changes characteristics were analyzed.[Result] The spatial distribution of each land use type in new costal area had distinct fractal characteristics.And,the amount and changes of three types of dimension values effectively revealed the changes of complicatedness,centeredness and evenness of spatial pattern of land use in the study area.The boundary dimension of unused land and salty earth increased incessantly,which suggested its increasing complicatedness.The boundary of the port and wharf and shoal land was getting simpler.The radius dimension of the cultivated land was larger than 2,which suggested that its area spread from center to the surroundings;the one in salty land and waters distributed evenly within different radius space to the center of the city;the one in other land use types reduced gradually from center to the surroundings.The information dimension value in the woodland and orchard land,unused land and shoal land was small,and was in obvious concentrated distribution;the spatial distribution of cultivated and salty land concentrated in the outside area;the construction area in the port and wharf spread gradually on the basis of original state;the spatial distribution of waters and residents and mines were even.[Conclusion] Applying fractal dimensions to the study of spatial pattern changes of urban land use can make up for some disadvantages in classical urban spatial pattern quantitative research,which has favorable practical value. 展开更多
关键词 Land use Fractal dimension Boundary dimension Radial dimension Information dimension tianjin New Coastal Area
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仙台病毒Tianjin株噬菌体Fab抗体库的构建及初步筛选
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作者 李霞 李晓绵 +1 位作者 石立莹 李梅 《中国生物制品学杂志》 CAS CSCD 2013年第4期482-485,共4页
目的构建仙台病毒Tianjin株噬菌体Fab抗体库,并进行初步筛选。方法用仙台病毒Tianjin株免疫小鼠,制备脾细胞悬液,提取小鼠脾细胞总RNA,RT-PCR扩增鼠抗体轻链(κ链)和重链Fd基因,将轻链基因和噬菌粒p3MH经SacⅠ和XbaⅠ双酶切后纯化回收,... 目的构建仙台病毒Tianjin株噬菌体Fab抗体库,并进行初步筛选。方法用仙台病毒Tianjin株免疫小鼠,制备脾细胞悬液,提取小鼠脾细胞总RNA,RT-PCR扩增鼠抗体轻链(κ链)和重链Fd基因,将轻链基因和噬菌粒p3MH经SacⅠ和XbaⅠ双酶切后纯化回收,经T4 DNA连接酶连接,电转化E.coli XL1-Blue,构建轻链库;将重链Fd段基因和轻链库p3MH-κ经SpeⅠ和XhoⅠ双酶切后,纯化回收并连接,电转化E.coli XL1-Blue,获得噬菌体抗体库,计算重组率和抗体库滴度。以仙台病毒Tianjin株重组蛋白HN2为抗原进行初步筛选,制备噬菌体抗体,并进行测序。结果构建的免疫噬菌体抗体库库容为1.18×107,重组率为90%,制备的噬菌体抗体滴度为1011pfu/ml;经初步筛选,噬菌体富集了约63.6倍,获得2株与重组蛋白HN2有结合活性的阳性克隆,经NCBI BLAST进行同源性分析,显示为鼠源性抗体,经IMGT分析,显示轻链基因与Vk9亚群基因的同源性为94.87%,重链基因与VH7亚群基因的同源性为97.85%。结论成功构建了仙台病毒Tianjin株噬菌体Fab抗体库,为该病毒株的诊断、疾病治疗及致病机制等研究奠定了基础。 展开更多
关键词 仙台病毒 tianjin FAB 噬菌体抗体库
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灭活仙台病毒Tianjin株诱导人乳腺癌细胞系BCAP37凋亡研究
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作者 韩哲 王顺达 +2 位作者 陈曦腾 赵杰 石立莹 《中华肿瘤防治杂志》 CAS 北大核心 2014年第8期565-569,共5页
目的:研究灭活仙台病毒Tianjin株诱导人乳腺癌细胞系BCAP37凋亡的作用。方法:用紫外线灭活不同浓度仙台病毒Tianjin株感染人乳腺癌BCAP37细胞,设立活病毒对照孔及正常细胞对照孔,感染后不同时间收集细胞,MTT法检测细胞存活率,琼脂糖凝... 目的:研究灭活仙台病毒Tianjin株诱导人乳腺癌细胞系BCAP37凋亡的作用。方法:用紫外线灭活不同浓度仙台病毒Tianjin株感染人乳腺癌BCAP37细胞,设立活病毒对照孔及正常细胞对照孔,感染后不同时间收集细胞,MTT法检测细胞存活率,琼脂糖凝胶电泳检测DNA片段降解情况,Hoechst染色观察细胞凋亡形态学变化,AnnexinⅤ/PI标记流式细胞仪检测细胞凋亡百分率,蛋白质印迹法检测Caspase-9及Caspase-3蛋白的表达。结果:MTT检测发现灭活仙台病毒Tianjin株对人乳腺癌细胞系BCAP37有明显的生长抑制作用,且呈剂量-时间依赖性;DNA片段琼脂糖凝胶电泳显示灭活病毒组呈典型的细胞凋亡梯形条带;Hoechst染色显示灭活病毒能够诱导BCAP37凋亡,显微镜下可见细胞核固缩,破裂,溶解,凋亡小体形成等形态学改变;流式细胞仪检测灭活病毒组细胞凋亡率为(15.1±1.1)%,明显高于正常细胞对照组,P<0.001;蛋白质印迹法结果显示,灭活病毒作用24h即出现Caspase-3及Caspase-9活化片段,与正常细胞组比较均明显升高,P值均<0.001。结论:灭活仙台病毒Tianjin株能够诱导人乳腺癌BCAP37细胞凋亡,其机制可能与Caspase-9和Caspase-3活化有关。 展开更多
关键词 仙台病毒tianjin 乳腺肿瘤 BCAP37 凋亡
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Analysis of Current Situation and Countermeasures of Plant Landscapes on Tianjin Urban Squares of China
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作者 孟瑾 陈良 +1 位作者 王月 刘志波 《Journal of Landscape Research》 2011年第11期29-31,35,共4页
Through the survey of plant landscapes on eight representative squares in Tianjin City,the paper had pointed out that plant landscapes on Tianjin squares were improper in terms of plants richness,cultivation layer and... Through the survey of plant landscapes on eight representative squares in Tianjin City,the paper had pointed out that plant landscapes on Tianjin squares were improper in terms of plants richness,cultivation layer and color change.It had suggested selecting regional characteristic tree species,applying salt-tolerant plants,emphasizing the diversity of plants species,highlighting the application of towering arbors,stressing the use of color-leaved plants,and creating abundant plant communities,so as to provide citizens with a more practical and ornamental square landscape environment through improving plant landscapes on squares in Tianjin City. 展开更多
关键词 tianjin City SQUARE Plant LANDSCAPE Current SITUATION COUNTERMEASURES
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副粘病毒Tianjin株F基因真核表达载体的构建及初步鉴定
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作者 张国际 李晓眠 +1 位作者 钟启平 李梅 《天津师范大学学报(自然科学版)》 CAS 2007年第3期19-21,共3页
为研究仙台病毒主要表面抗原f蛋白对小鼠的免疫效果,应用RT-PCR从副粘病毒Tianjin株基因组中扩增出F基因,将其插入pcDNA3的Pcmv下游,构建了F基因真核表达载体,同时使用PCR、限制性酶切和测序等方法对其进行了鉴定,鉴定结果证实构建成功... 为研究仙台病毒主要表面抗原f蛋白对小鼠的免疫效果,应用RT-PCR从副粘病毒Tianjin株基因组中扩增出F基因,将其插入pcDNA3的Pcmv下游,构建了F基因真核表达载体,同时使用PCR、限制性酶切和测序等方法对其进行了鉴定,鉴定结果证实构建成功.构建好的F基因真核表达载体可为下一步的体外表达和动物免疫实验奠定基础. 展开更多
关键词 副粘病毒tianjin F基因 真核表达载体
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副粘病毒Tianjin株RT-PCR检测方法的建立及初步应用
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作者 石立莹 李梅 +1 位作者 何健民 李晓眠 《热带医学杂志》 CAS 2009年第3期265-268,共4页
目的建立检测副粘病毒Tianjin株的RT-PCR方法,为临床标本的检测提供参考依据。方法根据副粘病毒Tianjin株F蛋白的基因序列,设计出一对特异性引物,以Tianjin株总RNA逆转录的cDNA为模板进行PCR扩增,然后对该方法的敏感性、特异性进行检测... 目的建立检测副粘病毒Tianjin株的RT-PCR方法,为临床标本的检测提供参考依据。方法根据副粘病毒Tianjin株F蛋白的基因序列,设计出一对特异性引物,以Tianjin株总RNA逆转录的cDNA为模板进行PCR扩增,然后对该方法的敏感性、特异性进行检测。并将该方法应用于临床84例下呼吸道感染患儿支气管肺泡灌洗液(BALF)标本的检测。结果通过此方法成功扩增出约500bp的预期条带。将Tianjin株鸡胚尿囊液(血凝效价为1:1280)稀释到320倍时仍可出现阳性条带,对新城疫病毒、甲型流感病毒鼠肺适应株、人副流感病毒Ⅰ型、柯萨奇病毒B3m株、单纯疱疹病毒Ⅰ型及鸡胚尿囊液的PCR检测结果均呈阴性。84例下呼吸道感染患儿BALF标本中,其中2例阳性,阳性率为2.38%,与本实验室建立的双抗体夹心ELISA法检测结果相接近。结论建立的副粘病毒Tianjin株RT-PCR检测方法是一种快速、灵敏、特异的方法,为患病儿童中Tianjin株感染情况的调查奠定了重要的基础。 展开更多
关键词 副粘病毒tianjin 仙台病毒 RT—PCR
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Statistical and causes analysis of storm surges along Tianjin coast during the past 20 years
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作者 李希彬 孙晓燕 +2 位作者 刘洋 张秋丰 牛福新 《Marine Science Bulletin》 CAS 2014年第1期15-24,共10页
Based on tidal data statistical analysis for 20 years of Tanggu Marine Environmental Monitoring Station from 1991 to 2010, we concluded that an average of nearly 10 days of 100 cm above water increase took place at Ti... Based on tidal data statistical analysis for 20 years of Tanggu Marine Environmental Monitoring Station from 1991 to 2010, we concluded that an average of nearly 10 days of 100 cm above water increase took place at Tianjin coast every year. The maximum high tide and average tide of Tianjin coast occurred in summer and autumn, and the maximum water increase also occurred in summer and autumn. Days with water increase more than 100 cm mostly occurred in spring, autumn and winter. Then we summarized the causes of coastal storm surge disaster in Tianjin based on astronomical tide factors, meteorological factors, sea level rise, land subsidence, and geographic factors, et al. Finally, we proposed storm surge disaster prevention measures. 展开更多
关键词 tianjin storm surge water increase statistical analysis cause analysis
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