Background： In the post-antibiotic era, essential oils （EO） are promising alternatives to growth-promoting antibiotics. The aim of the present study was to investigate the antibacterial activities of an EO product ...Background： In the post-antibiotic era, essential oils （EO） are promising alternatives to growth-promoting antibiotics. The aim of the present study was to investigate the antibacterial activities of an EO product and its components thymol and carvacrol in vitro, and the efficacy of EO to control Clostridium perffingens challenge in broiler chickens. Results： The in vitro minimum inhibitory concentration assay showed strong antibacterial activity of the EO product, thymol, and carvacrol against pathogenic Escherichia col~, C. perffingens, and Salmonella strains, and weak activity towards beneficial Lactobacillus strains. Besides, an additive effect was observed between thymol and carvacrol. The in vivo study was carried out with 448 male broiler chicks following a 4 x 2 factorial arrangement to test the effects of EO supplementation （0, 60, 120, or 240 mg/kg EO in wheat-based diet）, pathogen challenge （with or without oral gavage of C. perfr/ngens from day 14 to day 20） and their interactions. Each treatment consisted of eight replicate pens （seven birds/pen）. The challenge led to macroscopic gut lesions, and resulted in a significant increase in ileal populations of C. perffingens and Eschefichia subgroup （P ≤ 0.05） on day 21. Dietary EO supplementation did not influence C. perfringens numbers, but linearly alleviated intestinal lesions on day 21 and 28 （P= 0.010 and 0.036, respectively）, and decreased Eschefichia populations in ileum with increased EO dosages （P = 0.027 and 0.071 for day 21 and 28, respectively）. For caecum, EO quadratically influenced Lactobacillus populations on day 21 （P = 0.002）, and linearly decreased the numbers of total bacteria and Eschefichio on day 28 （P = 0.026 and 0.060, respectively）. Mean thymol and carvacrol concentrations in the small intestine were 0.21 and 0.20 μg/g in intestinal digesta （wet weight）, respectively, for birds fed 60 mg/kg EO, and 0.80 and 0.71 μg/g, respectively, for birds fed 240 mg/kg EO. Conclusions： These results indicated that dietary EO supplementation could affect intestinal microbiota and alleviate intestinal lesions in broilers, which may contribute in controlling C. perffingens infection in broiler chickens.展开更多
Background:Over the past years,antibiotic growth promoter had been restricted in animal husbandry production in many countries because of antimicrobial resistance and foodborne antibiotic residues.However,the problems...Background:Over the past years,antibiotic growth promoter had been restricted in animal husbandry production in many countries because of antimicrobial resistance and foodborne antibiotic residues.However,the problems of poor intestinal health and low growth efficiency of piglets have not been solved completely in an antibiotic-free diet,and it is urgent to explore alternatives to antimicrobial growth promoters.Methods:Here,a total of 532 weaned pigs were assigned to one of 4 treatments,the low amino acid(AA)level diet(d 1 to d 14 is 1.35%,d 15 to d 42 is 1.25%)(Low AA),the low AA level diet supplementation with a carvacrol-thymol blend(50 mg carvacrol and 50 mg thymol/kg of diet)(CB)(Low AA+CB),the high AA level diet(d 1 to d 14 is 1.50%,d 15 to d 42 is 1.40%)(High AA),and the high AA level diet supplementation with a CB(High AA+CB),respectively.Then we measured growth performance and intestinal health indicators of weaned pigs.Results:Results showed that high AA level significantly reduced plasma urea nitrogen,plasma Interleukin-6(IL-6)and fecal lipocalin-2 contents(P<0.05),significantly increased the relative abundance of fecal Lactobacillus and Enterococcus,and had a trend to increase the fecal secretory immunoglobulin A(sIgA)and mucin 2(MUC 2)contents(P<0.05)in piglets,thereby alleviating the diarrhea of piglets and reducing the feed conversion ratio(FCR)of piglets during d 1~14 after weaning.Dietary supplementation with CB significantly increased the activity of plasma antioxidant enzymes T-SOD and GSH-px(P<0.05),while significantly reduced plasma malondialdehyde(MDA),plasma interleukin-1β(IL-1β),plasma endotoxin and D-lactic acid contents(P<0.05).Meanwhile,CB significantly decreased fecal lipocalin-2 contents and the abundance of fecal Escherichia coli(P<0.05).Thus,we hypothesis that dietary supplementation with CB significantly increased the average daily gain(ADG)of piglets(P<0.05)during d 1~14 after weaning through promoting intestinal health.Conclusion:These results suggest that high AA level and dietary supplementation with CB improved the growth performance of weaned pigs in an antibiotic-free diet by improving AA metabolism and intestinal antioxidant capacity.展开更多
This study aimed to assess the preventive effects of thyme oil and thymol on doxorubicin(DOX)-induced renotoxicity,cardiotoxicity,and oxidative stress in Wistar rats.Thyme oil was subjected to GC-MS analysis,which ind...This study aimed to assess the preventive effects of thyme oil and thymol on doxorubicin(DOX)-induced renotoxicity,cardiotoxicity,and oxidative stress in Wistar rats.Thyme oil was subjected to GC-MS analysis,which indicated that thymol was the major constituent representing 33.896%.Rats intraperitoneally injected with DOX at a dose of 2 mg/kg b.w./one per week for 7 weeks were co-treated with thyme oil and its major constituent,thymol,at doses 250 and 100 mg/kg b.w./every other day,respectively,by oral gavage for the same period.Thyme oil and thymol markedly ameliorated the raised levels of serum urea,uric acid,and creatinine in DOX-administered rats.They also reduced the elevated activities of serum CK-MB and LDH.Thyme oil was more effective than thymol in decreasing the elevated serum creatinine level and serum CK-MB activity in DOX-administered rats,thereby reflecting its more potent effect on kidney and heart functions.Lipid peroxidation significantly decreased while GSH level and GST and GPx activities significantly increased in kidney and heart of DOX-administered rats treated with thyme oil and thymol.The DOX-induced perturbed kidney histological changes including congestion of glomerulus tuft,inflammatory cells infiltration,protein cast in lumina of the renal tubule,and thickening of the parietal layer of Bowman’s capsule were remarkably ameliorated as a result of treatment with thyme oil and thymol;thyme oil was more effective.In addition,DOX-induced deleterious heart histological alterations,including intramuscular infiltration of inflammatory cells,focal necrosis of cardiac myocytes,and edema,were remarkably reduced by treatment with thyme oil and thymol.Thus,it can be concluded that DOX could induce marked toxicity in kidney and heart,and the treatment with thyme oil or thymol produced potential improvement of kidney and heart function and histological integrity via repression of oxidative stress and enhancement of antioxidant defense mechanisms.展开更多
Objective To explore the anti-inflammatory effects and mechanisms of action of thymol in Aspergillus fumigatus(A.fumigatus)keratitis.Methods The minimum inhibitory concentration of thymol against A.fumigatus was detec...Objective To explore the anti-inflammatory effects and mechanisms of action of thymol in Aspergillus fumigatus(A.fumigatus)keratitis.Methods The minimum inhibitory concentration of thymol against A.fumigatus was detected.To characterize the anti-inflammatory effects of thymol,mouse corneas and human corneal epithelial cells were pretreated with thymol or dimethyl sulfoxide(DMSO)before infection with A.fumigatus spores.Slit-lamp microscopy,immunohistochemistry,myeloperoxidase detection,quantitative real-time polymerase chain reaction,and Western blotting were used to assess infection.Neutrophil and macrophage recruitment,in addition to the secretion of LOX-1 and IL-1β,were quantified to evaluate the relative contribution of thymol to the inflammatory response.Results We confirmed that the growth of A.fumigatus was directly inhibited by thymol.In contrast with the DMSO group,there was a lower degree of inflammation in the mouse corneas of the thymol-pretreated group.This was characterized by significantly lower clinical scores,less inflammatory cell infiltration,and lower expression of LOX-1 and IL-1β.Similarly,in vitro experiments indicated that the production of LOX-1 and IL-1βwas significantly inhibited after thymol treatment,in contrast with the DMSO-pretreated group.Conclusion Our findings demonstrate that thymol exerted a direct fungistatic activity on A.fumigatus.Furthermore,thymol played a protective role in fungal keratitis by inhibiting LOX-1/IL-1βsignaling pathway and reducing the recruitment of neutrophils and macrophages.展开更多
Mushrooms are good sources of phytochemicals that have antioxidant and anti-proliferative effects.This study identifi ed a unique isoform of 18S rRNA gene(864 bp)from a novel wild mushroom(SMK-1)(GenBank accession num...Mushrooms are good sources of phytochemicals that have antioxidant and anti-proliferative effects.This study identifi ed a unique isoform of 18S rRNA gene(864 bp)from a novel wild mushroom(SMK-1)(GenBank accession number:SUB3267363).Thin layer chromatographic(TLC)profiling of the methanolic extract of the dried mushroom fruiting bodies of SMK-1 revealed the presence of phenolic and fl avonoid fractions with retention factor(Rf)values of 0.955 and 0.927 respectively.The GC/MS chromatograms of the SMK-1 methanolic extract identifi ed the main bioactive compound was phenol,5-methyl-2-(1-methylethyl)(74.00%)(thymol).The radical scavenging activity for the fl avonoid fraction was greater than the phenolic fractions(Rf–phenolics fractions>Rf–fl avonoid fractions)with the antioxidant activity more than that of standard ascorbic acid.Also,the phenolic and flavonoid fractions of SMK-1 expressed cytotoxic effects in HeLa cells with IC50 values ranging from 5μg/mL to 80μg/mL in a dose-dependent manner.This present research highlights the presence of high thymol concentration in a novel wild mushroom that has antioxidant and anti-poliferative potential with therapeutic benefi ts.The application of thymol natural products from novel mushroom SMK-1 as nutrition supplements could inhibit oxidative stress triggered by numerous pathologies that may pave the way to develop a new therapeutic natural drug.展开更多
1 Introduction Centipeda minima（Compositae） is an annual herbaceous plant found in moist places throughout the plains mainly in the southern regions of the People＇s Republic of China, Eastern tropical Asia, Austra...1 Introduction Centipeda minima（Compositae） is an annual herbaceous plant found in moist places throughout the plains mainly in the southern regions of the People＇s Republic of China, Eastern tropical Asia, Australia and the Pacific Islands. It is a commonly used Chinese folk medicine for colds, whooping cough, nasal allergy, malaria and asthma. A number of sesquiterpene lactones, flavonoids and triterpenes have previously been isolated from C. minima. During our study on searching for biological active components from this plant,展开更多
In this work, a novel catalyst of Fe_3O_4@SiO_2@Yb_2O_3 was prepared and the degradation of thymol in reverse osmosis concentrate using ozonation was explored. The operational parameters, such as ozone dosage(8–48 mg...In this work, a novel catalyst of Fe_3O_4@SiO_2@Yb_2O_3 was prepared and the degradation of thymol in reverse osmosis concentrate using ozonation was explored. The operational parameters, such as ozone dosage(8–48 mg·min^(-1)),initial thymol concentration(20–100 mg·L^(-1)), initial pH value(3–11), and catalyst Fe_3O_4@SiO_2@Yb_2O_3dosage(0.2–1.0 g), were studied focusing on the thymol degradation and COD removal. The results indicated that the increase in ozone dosage, initial p H value, and Fe_3O_4@SiO_2@Yb_2O_3dosage accelerated the thymol degradation and COD removal, while the increase in initial thymol concentration hampered the effect of ozonation. A pathway of thymol degradation by catalytic ozonation was proposed based on the intermediates detected by gas chromatography-mass spectrometer and ion chromatography. This paper can provide basic data and technical alternative for pollutant removal from reverse osmosis concentrate by ozonation.展开更多
Anthracnose, caused by the fungus called <i><span style="font-family:Verdana;">Colletotrichum</span></i> <i><span style="font-family:Verdana;">gloeosporioides<...Anthracnose, caused by the fungus called <i><span style="font-family:Verdana;">Colletotrichum</span></i> <i><span style="font-family:Verdana;">gloeosporioides</span></i><span style="font-family:Verdana;">, is the main postharvest disease that affects mango production on Reunion Island. Fruit</span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">s</span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;"> for the export market are always treated with chemicals. The use of chemical treatment is not in adequation with consumer expectations, and the increasing emergence of fungicide-resistant isolates promotes the development of alternatives methods. The principal objective of this work was to use antimicrobial properties of thymol as an alternative postharvest treatment on mango. Thymol diluated in a penetrating agent solution was effective on mango anthracnose. At a concentration of 0.025%, Thymol limited necrosis </span><span style="font-family:Verdana;">development due to pathogens during fruit storage. This treatment can</span><span style="font-family:Verdana;"> stimulate some of polyphenols biosynthesis involved in the fruit resistance to postharvest disease, particularly the<span style="white-space:nowrap;">synthesis</span> of gallic acid and resorcinol. With this final concentration of 0.025% thymol, the treatment did not affect fruit maturation and quality, especially the peel colour and sugar content. Importantly, the treatment did not show any detectable effect on organoleptic qualities of the fruit.</span></span></span></span>展开更多
Thymol is one of the most important phytochemical components because of its pharmacological and bioactive potential effects. This review focuses particularly on thymol as an alternative natural antiparasitic with pote...Thymol is one of the most important phytochemical components because of its pharmacological and bioactive potential effects. This review focuses particularly on thymol as an alternative natural antiparasitic with potential use in the pharmaceutical industry. This is in line with the preferences of the natural products for treatment being safer and without side effects. The biosynthesis pathways of thymol and carvacrol have been discussed, and mechanism of action of thymol on parasites. Studies on thymol confirmed the activity of thymol as anti-parasite against anthelmintic, Trypanosoma ssp., Toxoplasma gondii, Leishmania spp., Plasmodium falciparum, Giardia duodenalis, Eimeria ssp., Cryptosporidium baileyi and Cryptosporidium galli.展开更多
[Objectives]To optimize the preparation process of 5-methyl-2-(2,4,4,7-tetramethylchromanyl)phenol(impurity C)and the liquid phase determination method of the impurity,and to provide an important basis for the establi...[Objectives]To optimize the preparation process of 5-methyl-2-(2,4,4,7-tetramethylchromanyl)phenol(impurity C)and the liquid phase determination method of the impurity,and to provide an important basis for the establishment of material methods and quality control of thymol.[Methods]The 4,7-dimethylcoumarin as the starting material was hydrolyzed by potassium hydroxide to obtain the substance II 5-methyl-2-(1-propylene-2-yl)phenol,and then purified and treated with hydrochloric acid to obtain a pale yellow solid.High-purity main peak was obtained by high-performance liquid phase method,and it was identified as impurity C by ^(1)H-NMR measurement and spectrum analysis.[Results]After the method was optimized,the synthesis route to the impurity C was simple,and the purity of impurity C was high.The obtained impurity C was confirmed by~1H-NMR to be a compound 5-methyl-2-(2,4,4,7-tetramethylchromanyl)phenol.The liquid phase detection method was successfully established to identify the compound.[Conclusions]The optimized method is easy to control and the impurity C has high purity and it is expected to provide quality assurance for the study of related substances in the quality control of thymol.展开更多
Pillared clay (PILC) was prepared from Moroccan clay and characterized, and its aqueous thymol adsorption capacities were studied using a batch equilibrium technique. So, we tested the encapsulation of thymol by alumi...Pillared clay (PILC) was prepared from Moroccan clay and characterized, and its aqueous thymol adsorption capacities were studied using a batch equilibrium technique. So, we tested the encapsulation of thymol by aluminum pillared clay (PILC). The PILCs displayed a total surface area of 270 m2/g, a total pore volume of 0.246 cm3/g and an average pore diameter of 8.9 A, which corresponds to the size of Al13 forming the pillars between the clay layers. The adsorption capacity shown by the PILCs for thymol from water is close to 319 mg?g-1 for low solid/liquid ratio (0.2%). This result suggests that the PILCs have both hydrophobic and hydrophilic characteristics, as a result of the presence of silanol and siloxane groups formed during the pillaring and calcination of the PILCs. The experimental data were analyzed by the Freundlich and the Langmuir isotherm types for low values of equilibrium concentration. The rise of the isotherm in this range of concentrations was related to the affinity of thymol for clay sites, and the equilibrium data fitted well with the Freundlich model with maximum adsorption capacity of 319.51 mg/g for a ratio RS/L = 0.2%. Pseudo-first and pseudo-second-order kinetic models were tested with the experimental data and pseudo-first order kinetics was the best for the adsorption of thymol with coefficients of correlation R2 ≥0.986, and the adsorption was rapid with 90% of the thymol adsorbed within the first 20 min.展开更多
Thymol, the key component of the thyme oil and its derivatives were evaluated for their structure activity relationship as fungicide against Rhizoctonia solani. Since plant-based chemicals are considered as “Generall...Thymol, the key component of the thyme oil and its derivatives were evaluated for their structure activity relationship as fungicide against Rhizoctonia solani. Since plant-based chemicals are considered as “Generally Recognized as Safe” (GRAS) chemicals, there is great potential to use those and synthetic derivatives against R. solani and other fungal pathogens, in vitro, and in the?greenhouse or field conditions. Among the six thymol derivatives evaluated, thymol acetate was considered as the most suitable commercially viable plant-based fungicide due to its superior efficacy as well as lowest residue.展开更多
Callus cultures of Origanum vulgare L. were established from leaf discus on Murashige and Skoog （MS） medium containing different levels of growth regulators, i.e., 2,4-Dichlorophenoxyacetic acid （2,4-D）, Naphthale...Callus cultures of Origanum vulgare L. were established from leaf discus on Murashige and Skoog （MS） medium containing different levels of growth regulators, i.e., 2,4-Dichlorophenoxyacetic acid （2,4-D）, Naphthalene acetic acid （NAA）, Benzyl Adenine （BA） and Kinetin （Kn） and incubated under dark condition. Callus tissues were employed to study the influence of abiotic elicitors on the production of thymol. Constant weights of callus （300 mg） were cultured on accumulation medium treated separately with each one of elicitors used （50 g/L sucrose, 200 mg/L NaC1 and 50 or 100 mg/L proline）. The fresh and dry weights of callus were recorded after six weeks. The result indicated that maximum production of fresh and dry callus weight were 1,014 mg and 46.20 mg respectively achieved at 0.5 mg/L 2,4-D and 3 mg/L BA adding to the medium. Dry callus tissues were extracted with 70% methanol and analyzed by HPLC to determine the concentrations of thymol. The addition of abiotic elicitors to MS medium caused significant reduction in fresh weight of callus compared with control treatment. The concentration of thymol in the callus cultured on control treatment was 146.6 ppm. The data showed that 50 or 100 mg/L proline produced the highest yield of thymol 181.48 ppm and 174.58 ppm respectively, followed by sucrose 162.9 ppm, whereas the treatment with NaCI caused reduction in thymol concentration to percentage of 50.56% compared with the control.展开更多
Aquaculture procedures such as capturing,handling,and trahsporting fish can lead to stress in animals.For this reason,anaesthesia has been recommended to reduce fish stress during these procedures.Synthetic and natura...Aquaculture procedures such as capturing,handling,and trahsporting fish can lead to stress in animals.For this reason,anaesthesia has been recommended to reduce fish stress during these procedures.Synthetic and natural anaesthetics such as tricaine methanesulfonate(MS-222)and eugenol(monoterpene)are commonly used;however,biochemical changes and aversive effects have been reported.Other monoterpene compounds such as thymol and menthol,have shown potential anaesthetic effects in fish species.Thus,further investigation of its anaesthetic properties seems imperative,and the use of the zebrafish in the early stages of its development is an appropriate model due to its advantages.Therefore,the aimed to evaluate the anaesthetic profile of thymol and menthol in zebrafish at 72 hpf(hours post fertisation).Following the determination of the effective anaesthetic concentration,three concentrations(100,200 and 300 mg/L for thymol and 200,400 and 500 mg/L for menthol)were further tested by focusing on mortality rate,heart rate,behaviour features of induction and oxidative effects in comparison to MS-222 and eugenol.Both monoterpenes caused anaesthetic effects in a concentration-dependent manner,with concentrations of 200 mg/L exhibiting a similar profile to MS 222 and eugenol,as evidenced by the increased recovery times.Furthermore,anaesthesia with thymol and menthol caused post-anaesthesia motor pattern abnormalities,which supported some indicators of stress.Slight modifications in physiological parameters and antioxidant changes were observed,but their association with thymol and menthol anaesthesia is unclear.Although sup-porting its anaesthetic use,more extensive research will be required to fully understand the impact of these natural anaesthetics on fish behaviour and stress levels,ultimately leading to safer and more successful anaes-thetic operations in the future.展开更多
It has been found that thymol (Thy) and rosmarinic acid (Ros-A) improve the growth performance of piglets and relieve intestinal inflammation in animals.The effects of Thy and Ros-A separately or in combination (Thy&#...It has been found that thymol (Thy) and rosmarinic acid (Ros-A) improve the growth performance of piglets and relieve intestinal inflammation in animals.The effects of Thy and Ros-A separately or in combination (Thy×Ros-A) on the intestinal function and health of piglets challenged with Escherichia coli K88 (E.coli K88) were investigated.A total of 30 piglets aged 21 d were assigned to 5 groups (n=6).The control (Con) and K88 groups piglets received a basal diet,while the Thy,Ros-A,and Thy×Ros-A groups were fed a basal diet supplemented with 500 mg/kg Thy,500 mg/kg Ros-A,and 250 mg/kg Thy+250 mg/kg Ros-A,respectively.On the 19th and 20th day,piglets in the K88,Thy,Ros-A,and Thy×Ros-A groups were orally administered 10 mL of phosphate-buffered saline (PBS) containing approximately 1×10^(9)CFU/mL of E.coli K88,while the Con group received an equal volume of PBS.The results showed that the Thy×Ros-A treatment reduced the damage to ileal villi induced by the E.coli K88 challenge,leading to longer villi in the ileum (P<0.05).Thy and Ros-A modulated the composition of the ileal microbiota.Compared to the K88 group,the Thy×Ros-A group had a higher abundance of Lactobacillus and Romboutsia,while Escherichia-Shigella and Desulforvibrio were lower (P<0.05).Additionally,the Thy×Ros-A group showed elevated levels of gene and protein expressions for zonula occludens-1,occludin,and claudin-1 compared to the K88 group (P<0.05).In conclusion,combining Thy and Ros-A reduced ileal damage and relieved the inflammation in weaned piglets challenged with E.coli K88 by regulating intestinal microflora and improving barrier function.展开更多
基金supported by Novus International Company(Missouri,USA)the earmarked fund for China Agricultural Research Systems(CARS-42)
文摘Background： In the post-antibiotic era, essential oils （EO） are promising alternatives to growth-promoting antibiotics. The aim of the present study was to investigate the antibacterial activities of an EO product and its components thymol and carvacrol in vitro, and the efficacy of EO to control Clostridium perffingens challenge in broiler chickens. Results： The in vitro minimum inhibitory concentration assay showed strong antibacterial activity of the EO product, thymol, and carvacrol against pathogenic Escherichia col~, C. perffingens, and Salmonella strains, and weak activity towards beneficial Lactobacillus strains. Besides, an additive effect was observed between thymol and carvacrol. The in vivo study was carried out with 448 male broiler chicks following a 4 x 2 factorial arrangement to test the effects of EO supplementation （0, 60, 120, or 240 mg/kg EO in wheat-based diet）, pathogen challenge （with or without oral gavage of C. perfr/ngens from day 14 to day 20） and their interactions. Each treatment consisted of eight replicate pens （seven birds/pen）. The challenge led to macroscopic gut lesions, and resulted in a significant increase in ileal populations of C. perffingens and Eschefichia subgroup （P ≤ 0.05） on day 21. Dietary EO supplementation did not influence C. perfringens numbers, but linearly alleviated intestinal lesions on day 21 and 28 （P= 0.010 and 0.036, respectively）, and decreased Eschefichia populations in ileum with increased EO dosages （P = 0.027 and 0.071 for day 21 and 28, respectively）. For caecum, EO quadratically influenced Lactobacillus populations on day 21 （P = 0.002）, and linearly decreased the numbers of total bacteria and Eschefichio on day 28 （P = 0.026 and 0.060, respectively）. Mean thymol and carvacrol concentrations in the small intestine were 0.21 and 0.20 μg/g in intestinal digesta （wet weight）, respectively, for birds fed 60 mg/kg EO, and 0.80 and 0.71 μg/g, respectively, for birds fed 240 mg/kg EO. Conclusions： These results indicated that dietary EO supplementation could affect intestinal microbiota and alleviate intestinal lesions in broilers, which may contribute in controlling C. perffingens infection in broiler chickens.
基金supported by Hubei Province Science and Technology Innovation Major Project(2019ABA081,2021BBA083)China Agriculture Research System of MOF and MARA.
文摘Background:Over the past years,antibiotic growth promoter had been restricted in animal husbandry production in many countries because of antimicrobial resistance and foodborne antibiotic residues.However,the problems of poor intestinal health and low growth efficiency of piglets have not been solved completely in an antibiotic-free diet,and it is urgent to explore alternatives to antimicrobial growth promoters.Methods:Here,a total of 532 weaned pigs were assigned to one of 4 treatments,the low amino acid(AA)level diet(d 1 to d 14 is 1.35%,d 15 to d 42 is 1.25%)(Low AA),the low AA level diet supplementation with a carvacrol-thymol blend(50 mg carvacrol and 50 mg thymol/kg of diet)(CB)(Low AA+CB),the high AA level diet(d 1 to d 14 is 1.50%,d 15 to d 42 is 1.40%)(High AA),and the high AA level diet supplementation with a CB(High AA+CB),respectively.Then we measured growth performance and intestinal health indicators of weaned pigs.Results:Results showed that high AA level significantly reduced plasma urea nitrogen,plasma Interleukin-6(IL-6)and fecal lipocalin-2 contents(P<0.05),significantly increased the relative abundance of fecal Lactobacillus and Enterococcus,and had a trend to increase the fecal secretory immunoglobulin A(sIgA)and mucin 2(MUC 2)contents(P<0.05)in piglets,thereby alleviating the diarrhea of piglets and reducing the feed conversion ratio(FCR)of piglets during d 1~14 after weaning.Dietary supplementation with CB significantly increased the activity of plasma antioxidant enzymes T-SOD and GSH-px(P<0.05),while significantly reduced plasma malondialdehyde(MDA),plasma interleukin-1β(IL-1β),plasma endotoxin and D-lactic acid contents(P<0.05).Meanwhile,CB significantly decreased fecal lipocalin-2 contents and the abundance of fecal Escherichia coli(P<0.05).Thus,we hypothesis that dietary supplementation with CB significantly increased the average daily gain(ADG)of piglets(P<0.05)during d 1~14 after weaning through promoting intestinal health.Conclusion:These results suggest that high AA level and dietary supplementation with CB improved the growth performance of weaned pigs in an antibiotic-free diet by improving AA metabolism and intestinal antioxidant capacity.
文摘This study aimed to assess the preventive effects of thyme oil and thymol on doxorubicin(DOX)-induced renotoxicity,cardiotoxicity,and oxidative stress in Wistar rats.Thyme oil was subjected to GC-MS analysis,which indicated that thymol was the major constituent representing 33.896%.Rats intraperitoneally injected with DOX at a dose of 2 mg/kg b.w./one per week for 7 weeks were co-treated with thyme oil and its major constituent,thymol,at doses 250 and 100 mg/kg b.w./every other day,respectively,by oral gavage for the same period.Thyme oil and thymol markedly ameliorated the raised levels of serum urea,uric acid,and creatinine in DOX-administered rats.They also reduced the elevated activities of serum CK-MB and LDH.Thyme oil was more effective than thymol in decreasing the elevated serum creatinine level and serum CK-MB activity in DOX-administered rats,thereby reflecting its more potent effect on kidney and heart functions.Lipid peroxidation significantly decreased while GSH level and GST and GPx activities significantly increased in kidney and heart of DOX-administered rats treated with thyme oil and thymol.The DOX-induced perturbed kidney histological changes including congestion of glomerulus tuft,inflammatory cells infiltration,protein cast in lumina of the renal tubule,and thickening of the parietal layer of Bowman’s capsule were remarkably ameliorated as a result of treatment with thyme oil and thymol;thyme oil was more effective.In addition,DOX-induced deleterious heart histological alterations,including intramuscular infiltration of inflammatory cells,focal necrosis of cardiac myocytes,and edema,were remarkably reduced by treatment with thyme oil and thymol.Thus,it can be concluded that DOX could induce marked toxicity in kidney and heart,and the treatment with thyme oil or thymol produced potential improvement of kidney and heart function and histological integrity via repression of oxidative stress and enhancement of antioxidant defense mechanisms.
基金the National Natural Science Foundation of China(No.82171019)Natural Science Foundation of Shandong Province(No.ZR2021MH368)+1 种基金Traditional Chinese Medicine Research Project of Qingdao(No.2020-zyy055)Shandong Qingdao Outstanding Health Professional Development Fund.
文摘Objective To explore the anti-inflammatory effects and mechanisms of action of thymol in Aspergillus fumigatus(A.fumigatus)keratitis.Methods The minimum inhibitory concentration of thymol against A.fumigatus was detected.To characterize the anti-inflammatory effects of thymol,mouse corneas and human corneal epithelial cells were pretreated with thymol or dimethyl sulfoxide(DMSO)before infection with A.fumigatus spores.Slit-lamp microscopy,immunohistochemistry,myeloperoxidase detection,quantitative real-time polymerase chain reaction,and Western blotting were used to assess infection.Neutrophil and macrophage recruitment,in addition to the secretion of LOX-1 and IL-1β,were quantified to evaluate the relative contribution of thymol to the inflammatory response.Results We confirmed that the growth of A.fumigatus was directly inhibited by thymol.In contrast with the DMSO group,there was a lower degree of inflammation in the mouse corneas of the thymol-pretreated group.This was characterized by significantly lower clinical scores,less inflammatory cell infiltration,and lower expression of LOX-1 and IL-1β.Similarly,in vitro experiments indicated that the production of LOX-1 and IL-1βwas significantly inhibited after thymol treatment,in contrast with the DMSO-pretreated group.Conclusion Our findings demonstrate that thymol exerted a direct fungistatic activity on A.fumigatus.Furthermore,thymol played a protective role in fungal keratitis by inhibiting LOX-1/IL-1βsignaling pathway and reducing the recruitment of neutrophils and macrophages.
文摘Mushrooms are good sources of phytochemicals that have antioxidant and anti-proliferative effects.This study identifi ed a unique isoform of 18S rRNA gene(864 bp)from a novel wild mushroom(SMK-1)(GenBank accession number:SUB3267363).Thin layer chromatographic(TLC)profiling of the methanolic extract of the dried mushroom fruiting bodies of SMK-1 revealed the presence of phenolic and fl avonoid fractions with retention factor(Rf)values of 0.955 and 0.927 respectively.The GC/MS chromatograms of the SMK-1 methanolic extract identifi ed the main bioactive compound was phenol,5-methyl-2-(1-methylethyl)(74.00%)(thymol).The radical scavenging activity for the fl avonoid fraction was greater than the phenolic fractions(Rf–phenolics fractions>Rf–fl avonoid fractions)with the antioxidant activity more than that of standard ascorbic acid.Also,the phenolic and flavonoid fractions of SMK-1 expressed cytotoxic effects in HeLa cells with IC50 values ranging from 5μg/mL to 80μg/mL in a dose-dependent manner.This present research highlights the presence of high thymol concentration in a novel wild mushroom that has antioxidant and anti-poliferative potential with therapeutic benefi ts.The application of thymol natural products from novel mushroom SMK-1 as nutrition supplements could inhibit oxidative stress triggered by numerous pathologies that may pave the way to develop a new therapeutic natural drug.
基金Supported by the Natural Science Foundation of Tianjin, China(No.07JCZDJC05400).
文摘1 Introduction Centipeda minima（Compositae） is an annual herbaceous plant found in moist places throughout the plains mainly in the southern regions of the People＇s Republic of China, Eastern tropical Asia, Australia and the Pacific Islands. It is a commonly used Chinese folk medicine for colds, whooping cough, nasal allergy, malaria and asthma. A number of sesquiterpene lactones, flavonoids and triterpenes have previously been isolated from C. minima. During our study on searching for biological active components from this plant,
基金Supported by the National Natural Science Foundation of China(51138008,51478314,51308391)the Key Project for Research and Development(2016YFC0400503)+1 种基金the Tianjin Science and Technology Program(14ZCDGSF00128)the Natural Science Foundation of Tianjin(14JCQNJC09000)
文摘In this work, a novel catalyst of Fe_3O_4@SiO_2@Yb_2O_3 was prepared and the degradation of thymol in reverse osmosis concentrate using ozonation was explored. The operational parameters, such as ozone dosage(8–48 mg·min^(-1)),initial thymol concentration(20–100 mg·L^(-1)), initial pH value(3–11), and catalyst Fe_3O_4@SiO_2@Yb_2O_3dosage(0.2–1.0 g), were studied focusing on the thymol degradation and COD removal. The results indicated that the increase in ozone dosage, initial p H value, and Fe_3O_4@SiO_2@Yb_2O_3dosage accelerated the thymol degradation and COD removal, while the increase in initial thymol concentration hampered the effect of ozonation. A pathway of thymol degradation by catalytic ozonation was proposed based on the intermediates detected by gas chromatography-mass spectrometer and ion chromatography. This paper can provide basic data and technical alternative for pollutant removal from reverse osmosis concentrate by ozonation.
文摘Anthracnose, caused by the fungus called <i><span style="font-family:Verdana;">Colletotrichum</span></i> <i><span style="font-family:Verdana;">gloeosporioides</span></i><span style="font-family:Verdana;">, is the main postharvest disease that affects mango production on Reunion Island. Fruit</span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">s</span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;"> for the export market are always treated with chemicals. The use of chemical treatment is not in adequation with consumer expectations, and the increasing emergence of fungicide-resistant isolates promotes the development of alternatives methods. The principal objective of this work was to use antimicrobial properties of thymol as an alternative postharvest treatment on mango. Thymol diluated in a penetrating agent solution was effective on mango anthracnose. At a concentration of 0.025%, Thymol limited necrosis </span><span style="font-family:Verdana;">development due to pathogens during fruit storage. This treatment can</span><span style="font-family:Verdana;"> stimulate some of polyphenols biosynthesis involved in the fruit resistance to postharvest disease, particularly the<span style="white-space:nowrap;">synthesis</span> of gallic acid and resorcinol. With this final concentration of 0.025% thymol, the treatment did not affect fruit maturation and quality, especially the peel colour and sugar content. Importantly, the treatment did not show any detectable effect on organoleptic qualities of the fruit.</span></span></span></span>
文摘Thymol is one of the most important phytochemical components because of its pharmacological and bioactive potential effects. This review focuses particularly on thymol as an alternative natural antiparasitic with potential use in the pharmaceutical industry. This is in line with the preferences of the natural products for treatment being safer and without side effects. The biosynthesis pathways of thymol and carvacrol have been discussed, and mechanism of action of thymol on parasites. Studies on thymol confirmed the activity of thymol as anti-parasite against anthelmintic, Trypanosoma ssp., Toxoplasma gondii, Leishmania spp., Plasmodium falciparum, Giardia duodenalis, Eimeria ssp., Cryptosporidium baileyi and Cryptosporidium galli.
文摘[Objectives]To optimize the preparation process of 5-methyl-2-(2,4,4,7-tetramethylchromanyl)phenol(impurity C)and the liquid phase determination method of the impurity,and to provide an important basis for the establishment of material methods and quality control of thymol.[Methods]The 4,7-dimethylcoumarin as the starting material was hydrolyzed by potassium hydroxide to obtain the substance II 5-methyl-2-(1-propylene-2-yl)phenol,and then purified and treated with hydrochloric acid to obtain a pale yellow solid.High-purity main peak was obtained by high-performance liquid phase method,and it was identified as impurity C by ^(1)H-NMR measurement and spectrum analysis.[Results]After the method was optimized,the synthesis route to the impurity C was simple,and the purity of impurity C was high.The obtained impurity C was confirmed by~1H-NMR to be a compound 5-methyl-2-(2,4,4,7-tetramethylchromanyl)phenol.The liquid phase detection method was successfully established to identify the compound.[Conclusions]The optimized method is easy to control and the impurity C has high purity and it is expected to provide quality assurance for the study of related substances in the quality control of thymol.
文摘Pillared clay (PILC) was prepared from Moroccan clay and characterized, and its aqueous thymol adsorption capacities were studied using a batch equilibrium technique. So, we tested the encapsulation of thymol by aluminum pillared clay (PILC). The PILCs displayed a total surface area of 270 m2/g, a total pore volume of 0.246 cm3/g and an average pore diameter of 8.9 A, which corresponds to the size of Al13 forming the pillars between the clay layers. The adsorption capacity shown by the PILCs for thymol from water is close to 319 mg?g-1 for low solid/liquid ratio (0.2%). This result suggests that the PILCs have both hydrophobic and hydrophilic characteristics, as a result of the presence of silanol and siloxane groups formed during the pillaring and calcination of the PILCs. The experimental data were analyzed by the Freundlich and the Langmuir isotherm types for low values of equilibrium concentration. The rise of the isotherm in this range of concentrations was related to the affinity of thymol for clay sites, and the equilibrium data fitted well with the Freundlich model with maximum adsorption capacity of 319.51 mg/g for a ratio RS/L = 0.2%. Pseudo-first and pseudo-second-order kinetic models were tested with the experimental data and pseudo-first order kinetics was the best for the adsorption of thymol with coefficients of correlation R2 ≥0.986, and the adsorption was rapid with 90% of the thymol adsorbed within the first 20 min.
文摘Thymol, the key component of the thyme oil and its derivatives were evaluated for their structure activity relationship as fungicide against Rhizoctonia solani. Since plant-based chemicals are considered as “Generally Recognized as Safe” (GRAS) chemicals, there is great potential to use those and synthetic derivatives against R. solani and other fungal pathogens, in vitro, and in the?greenhouse or field conditions. Among the six thymol derivatives evaluated, thymol acetate was considered as the most suitable commercially viable plant-based fungicide due to its superior efficacy as well as lowest residue.
文摘Callus cultures of Origanum vulgare L. were established from leaf discus on Murashige and Skoog （MS） medium containing different levels of growth regulators, i.e., 2,4-Dichlorophenoxyacetic acid （2,4-D）, Naphthalene acetic acid （NAA）, Benzyl Adenine （BA） and Kinetin （Kn） and incubated under dark condition. Callus tissues were employed to study the influence of abiotic elicitors on the production of thymol. Constant weights of callus （300 mg） were cultured on accumulation medium treated separately with each one of elicitors used （50 g/L sucrose, 200 mg/L NaC1 and 50 or 100 mg/L proline）. The fresh and dry weights of callus were recorded after six weeks. The result indicated that maximum production of fresh and dry callus weight were 1,014 mg and 46.20 mg respectively achieved at 0.5 mg/L 2,4-D and 3 mg/L BA adding to the medium. Dry callus tissues were extracted with 70% methanol and analyzed by HPLC to determine the concentrations of thymol. The addition of abiotic elicitors to MS medium caused significant reduction in fresh weight of callus compared with control treatment. The concentration of thymol in the callus cultured on control treatment was 146.6 ppm. The data showed that 50 or 100 mg/L proline produced the highest yield of thymol 181.48 ppm and 174.58 ppm respectively, followed by sucrose 162.9 ppm, whereas the treatment with NaCI caused reduction in thymol concentration to percentage of 50.56% compared with the control.
基金supported by Portuguese funds by FCT/MCTES-Portuguese Foundation for Science and Technology/Ministerio da Ciencia,Tecnologia e Ensino Superior,under the project 10.54499/UIDB/04033/2020(CITAB—Centre for Research and Technology of Agro-Environment and Biological Sciences),SFRH/BD/144904/2019 and 10.54499/2021.00458.CEECIND/CP1690/CT0001.
文摘Aquaculture procedures such as capturing,handling,and trahsporting fish can lead to stress in animals.For this reason,anaesthesia has been recommended to reduce fish stress during these procedures.Synthetic and natural anaesthetics such as tricaine methanesulfonate(MS-222)and eugenol(monoterpene)are commonly used;however,biochemical changes and aversive effects have been reported.Other monoterpene compounds such as thymol and menthol,have shown potential anaesthetic effects in fish species.Thus,further investigation of its anaesthetic properties seems imperative,and the use of the zebrafish in the early stages of its development is an appropriate model due to its advantages.Therefore,the aimed to evaluate the anaesthetic profile of thymol and menthol in zebrafish at 72 hpf(hours post fertisation).Following the determination of the effective anaesthetic concentration,three concentrations(100,200 and 300 mg/L for thymol and 200,400 and 500 mg/L for menthol)were further tested by focusing on mortality rate,heart rate,behaviour features of induction and oxidative effects in comparison to MS-222 and eugenol.Both monoterpenes caused anaesthetic effects in a concentration-dependent manner,with concentrations of 200 mg/L exhibiting a similar profile to MS 222 and eugenol,as evidenced by the increased recovery times.Furthermore,anaesthesia with thymol and menthol caused post-anaesthesia motor pattern abnormalities,which supported some indicators of stress.Slight modifications in physiological parameters and antioxidant changes were observed,but their association with thymol and menthol anaesthesia is unclear.Although sup-porting its anaesthetic use,more extensive research will be required to fully understand the impact of these natural anaesthetics on fish behaviour and stress levels,ultimately leading to safer and more successful anaes-thetic operations in the future.
基金Key R&D Program of China(2017YFE0135200)supported this study.
文摘It has been found that thymol (Thy) and rosmarinic acid (Ros-A) improve the growth performance of piglets and relieve intestinal inflammation in animals.The effects of Thy and Ros-A separately or in combination (Thy×Ros-A) on the intestinal function and health of piglets challenged with Escherichia coli K88 (E.coli K88) were investigated.A total of 30 piglets aged 21 d were assigned to 5 groups (n=6).The control (Con) and K88 groups piglets received a basal diet,while the Thy,Ros-A,and Thy×Ros-A groups were fed a basal diet supplemented with 500 mg/kg Thy,500 mg/kg Ros-A,and 250 mg/kg Thy+250 mg/kg Ros-A,respectively.On the 19th and 20th day,piglets in the K88,Thy,Ros-A,and Thy×Ros-A groups were orally administered 10 mL of phosphate-buffered saline (PBS) containing approximately 1×10^(9)CFU/mL of E.coli K88,while the Con group received an equal volume of PBS.The results showed that the Thy×Ros-A treatment reduced the damage to ileal villi induced by the E.coli K88 challenge,leading to longer villi in the ileum (P<0.05).Thy and Ros-A modulated the composition of the ileal microbiota.Compared to the K88 group,the Thy×Ros-A group had a higher abundance of Lactobacillus and Romboutsia,while Escherichia-Shigella and Desulforvibrio were lower (P<0.05).Additionally,the Thy×Ros-A group showed elevated levels of gene and protein expressions for zonula occludens-1,occludin,and claudin-1 compared to the K88 group (P<0.05).In conclusion,combining Thy and Ros-A reduced ileal damage and relieved the inflammation in weaned piglets challenged with E.coli K88 by regulating intestinal microflora and improving barrier function.
