Objective Calibrated Automated Thrombogram（CAT） is a test to monitor the generation of thrombin. It can be described by four parameters： lag time, peak thrombin, endogenous thrombin potential （ETP） and time to pe...Objective Calibrated Automated Thrombogram（CAT） is a test to monitor the generation of thrombin. It can be described by four parameters： lag time, peak thrombin, endogenous thrombin potential （ETP） and time to peak （ttPeak）. This study aims to determine the normal ranges of CAT parameters in Chinese, and evaluate whether thrombin generation is correlated with the concentration of heparin/Iow molecular weight heparin. Methods Plasma from 120 healthy subjects were collected to determine the normal rangea of CAT parameters in Chinese. Normal plasma pool （NPP, n=25） spiked with different concentrations of heparin or enoxaparin were used to detecte CAT parameters. The overall and age specific normal ranges of CAT parameters were calculated using descriptive statistics method with mean＋-2SD. The correlation between CAT parameters and age or concentrations of heparin, enoxaparin were analyzed with linear regression model. Results The normal ranges for lag time, peak thrombin, ETP, ttPeak in the subjects were 3.648＋2.465 min, 367.39＋151.93 nmol/L, 2277＋_1030 nmol/L.min and 6.372＋_4.280 min respectively. Age was linearly correlated with lag time （r=-0.6583, P〈0.0002）, peak thrombin （r=0.4863, P〈0.0002）, ETP （r=0.3608, P〈0.0014） and ttPeak （r=-0.6323, P〈0.0002）. The values of ETP/peak ratio were linearly correlated with concentrations of heparin. Conclusion The normal ranges of four CAT parameters for Chinese were determined. CAT parameters are associated with age. ETP/peak ratio could be used to monitor the process of anticoagulation therapy.展开更多
基金supported by grant from the Ministry of Education of China(grant No.20110001120088)
文摘Objective Calibrated Automated Thrombogram（CAT） is a test to monitor the generation of thrombin. It can be described by four parameters： lag time, peak thrombin, endogenous thrombin potential （ETP） and time to peak （ttPeak）. This study aims to determine the normal ranges of CAT parameters in Chinese, and evaluate whether thrombin generation is correlated with the concentration of heparin/Iow molecular weight heparin. Methods Plasma from 120 healthy subjects were collected to determine the normal rangea of CAT parameters in Chinese. Normal plasma pool （NPP, n=25） spiked with different concentrations of heparin or enoxaparin were used to detecte CAT parameters. The overall and age specific normal ranges of CAT parameters were calculated using descriptive statistics method with mean＋-2SD. The correlation between CAT parameters and age or concentrations of heparin, enoxaparin were analyzed with linear regression model. Results The normal ranges for lag time, peak thrombin, ETP, ttPeak in the subjects were 3.648＋2.465 min, 367.39＋151.93 nmol/L, 2277＋_1030 nmol/L.min and 6.372＋_4.280 min respectively. Age was linearly correlated with lag time （r=-0.6583, P〈0.0002）, peak thrombin （r=0.4863, P〈0.0002）, ETP （r=0.3608, P〈0.0014） and ttPeak （r=-0.6323, P〈0.0002）. The values of ETP/peak ratio were linearly correlated with concentrations of heparin. Conclusion The normal ranges of four CAT parameters for Chinese were determined. CAT parameters are associated with age. ETP/peak ratio could be used to monitor the process of anticoagulation therapy.
文摘目的 分析血栓弹力图、凝血功能指标与急性缺血性脑卒中(acute ischemic stroke, AIS)患者病情严重程度的相关性。方法 回顾性选取广东省第二人民医院(民航院区)于2023年7月—2024年1月收治的60例AIS患者的临床资料,并以美国国立卫生研究院卒中评分量表(National Institutes of Health Stroke Scale, NIHSS)将患者分为轻症组(33例)与中重症组(27例)。比较两组的血栓弹力图(thromboelastography, TEG)相关指标与凝血功能指标,并通过Pearson相关分析TEG相关指标、凝血功能与病情程度的相关性。结果 中重症组凝血因子功能(reaction time, R值)、纤维蛋白聚合时间(kinetics, K值)、凝血酶原时间(prothrombin time, PT)、凝血酶时间(thrombin time, TT)、活化部分凝血活酶时间(activated partial thromboplastin time, APTT)均低于轻症组,差异有统计学意义(P均<0.05);中重症组血小板功能(maximum amplitude, MA)、纤维蛋白聚合速度(Angle)及纤维蛋白原(fibrinogen, FIB)分别为(78.25±4.78)mm、(77.93±3.96)°、(5.56±1.46)g/L,高于轻症组的(65.16±5.31)mm、(69.12±4.11)°、(3.02±0.97)g/L,差异有统计学意义(t=9.931、8.396、8.060,P均<0.05)。经Pearson相关分析显示,TEG相关指标R值、K值及凝血功能指标PT、TT、APTT与AIS患者病情严重程度呈负相关,TEG相关指标MA、Angle及凝血功能指标FIB与AIS患者病情严重程度呈正相关(P均<0.05)。结论TEG相关指标及凝血功能指标与AIS患者病情严重程度具有明显相关性,对中重度AIS患者具有较好的诊断价值。
