Background:The medicinal material known as Os Draconis(Longgu)originates from fossilized remains of ancient mammals and is widely used in treating emotional and mental conditions.However,fossil resources are nonrenewa...Background:The medicinal material known as Os Draconis(Longgu)originates from fossilized remains of ancient mammals and is widely used in treating emotional and mental conditions.However,fossil resources are nonrenewable,and clinical demand is increasingly difficult to meet,leading to a proliferation of counterfeit products.During prolonged geological burial,static pressure from the surrounding strata severely compromises the microstructural integrity of osteons in Os Draconis,but Os Draconis still largely retains the structural features of mammalian bone.Methods:Using verified authentic Os Draconis samples over 10,000 years old as a baseline,this study summarizes the ultrastructural characteristics of genuine Os Draconis.Employing electron probe microanalysis and optical polarized light microscopy,we examined 28 batches of authentic Os Draconis and 31 batches of counterfeits to identify their ultrastructural differences.Key points for ultrastructural identification of Os Draconis were compiled,and a new identification approach was proposed based on these differences.Results:Authentic Os Draconis exhibited distinct ultrastructural markers:irregularly shaped osteons with traversing fissures,deformed/displaced Haversian canals,and secondary mineral infill(predominantly calcium carbonate).Counterfeits showed regular osteon arrangements,absent traversal fissures,and homogeneous hydroxyapatite composition.Lab-simulated samples lacked structural degradation features.EPMA confirmed calcium carbonate infill in fossilized Haversian canals,while elemental profiles differentiated lacunae types(void vs.mineral-packed).Conclusion:The study established ultrastructural criteria for authentic Os Draconis identification:osteon deformation,geological fissures penetrating bone units,and heterogenous mineral deposition.These features,unattainable in counterfeits or modern processed bones,provide a cost-effective,accurate identification method.This approach bridges gaps in TCM material standardization and supports quality control for clinical applications.展开更多
3D laser scanning technology is widely used in underground openings for high-precision,rapid,and nondestructive structural evaluations.Segmenting large 3D point cloud datasets,particularly in coal mine roadways with m...3D laser scanning technology is widely used in underground openings for high-precision,rapid,and nondestructive structural evaluations.Segmenting large 3D point cloud datasets,particularly in coal mine roadways with multi-scale targets,remains challenging.This paper proposes an enhanced segmentation method integrating improved PointNet++with a coverage-voted strategy.The coverage-voted strategy reduces data while preserving multi-scale target topology.The segmentation is achieved using an enhanced PointNet++algorithm with a normalization preprocessing head,resulting in a 94%accuracy for common supporting components.Ablation experiments show that the preprocessing head and coverage strategies increase segmentation accuracy by 20%and 2%,respectively,and improve Intersection over Union(IoU)for bearing plate segmentation by 58%and 20%.The accuracy of the current pretraining segmentation model may be affected by variations in surface support components,but it can be readily enhanced through re-optimization with additional labeled point cloud data.This proposed method,combined with a previously developed machine learning model that links rock bolt load and the deformation field of its bearing plate,provides a robust technique for simultaneously measuring the load of multiple rock bolts in a single laser scan.展开更多
AIM:To construct an intelligent segmentation scheme for precise localization of central serous chorioretinopathy(CSC)leakage points,thereby enabling ophthalmologists to deliver accurate laser treatment without navigat...AIM:To construct an intelligent segmentation scheme for precise localization of central serous chorioretinopathy(CSC)leakage points,thereby enabling ophthalmologists to deliver accurate laser treatment without navigational laser equipment.METHODS:A dataset with dual labels(point-level and pixel-level)was first established based on fundus fluorescein angiography(FFA)images of CSC and subsequently divided into training(102 images),validation(40 images),and test(40 images)datasets.An intelligent segmentation method was then developed,based on the You Only Look Once version 8 Pose Estimation(YOLOv8-Pose)model and segment anything model(SAM),to segment CSC leakage points.Next,the YOLOv8-Pose model was trained for 200 epochs,and the best-performing model was selected to form the optimal combination with SAM.Additionally,the classic five types of U-Net series models[i.e.,U-Net,recurrent residual U-Net(R2U-Net),attention U-Net(AttU-Net),recurrent residual attention U-Net(R2AttUNet),and nested U-Net(UNet^(++))]were initialized with three random seeds and trained for 200 epochs,resulting in a total of 15 baseline models for comparison.Finally,based on the metrics including Dice similarity coefficient(DICE),intersection over union(IoU),precision,recall,precisionrecall(PR)curve,and receiver operating characteristic(ROC)curve,the proposed method was compared with baseline models through quantitative and qualitative experiments for leakage point segmentation,thereby demonstrating its effectiveness.RESULTS:With the increase of training epochs,the mAP50-95,Recall,and precision of the YOLOv8-Pose model showed a significant increase and tended to stabilize,and it achieved a preliminary localization success rate of 90%(i.e.,36 images)for CSC leakage points in 40 test images.Using manually expert-annotated pixel-level labels as the ground truth,the proposed method achieved outcomes with a DICE of 57.13%,an IoU of 45.31%,a precision of 45.91%,a recall of 93.57%,an area under the PR curve(AUC-PR)of 0.78 and an area under the ROC curve(AUC-ROC)of 0.97,which enables more accurate segmentation of CSC leakage points.CONCLUSION:By combining the precise localization capability of the YOLOv8-Pose model with the robust and flexible segmentation ability of SAM,the proposed method not only demonstrates the effectiveness of the YOLOv8-Pose model in detecting keypoint coordinates of CSC leakage points from the perspective of application innovation but also establishes a novel approach for accurate segmentation of CSC leakage points through the“detect-then-segment”strategy,thereby providing a potential auxiliary means for the automatic and precise realtime localization of leakage points during traditional laser photocoagulation for CSC.展开更多
Highlights By conjugating the same anti-N monoclonal antibody(mAb4-mAb1)with colloidal gold or fluorescent microspheres,this study developed two rapid point-of-care antigen immunochromatographic strips for the detecti...Highlights By conjugating the same anti-N monoclonal antibody(mAb4-mAb1)with colloidal gold or fluorescent microspheres,this study developed two rapid point-of-care antigen immunochromatographic strips for the detection of porcine deltacoronavirus.The fluorescent microsphere-based lateral flow test strip demonstrated a sensitivity of 10^(1.7)TCID_(50)/0.1 mL,which is fourfold higher than that of the colloidal gold-based assay.Porcine deltacoronavirus(PDCoV)is a recently identified enteric coronavirus that causes an acute infectious disease in piglets,leading to diarrhea,vomiting,dehydration,and mortality(Hu et al.2015).展开更多
针对菇房内杏鲍菇表型参数测量任务中,由于扫描设备视角受限,扫描的杏鲍菇点云出现残缺问题,基于AdaPoinTr(Adaptive geometry-aware point transformers)提出了改进的SwinPoinTr模型,实现了对残缺杏鲍菇点云的准确补全和杏鲍菇表型参...针对菇房内杏鲍菇表型参数测量任务中,由于扫描设备视角受限,扫描的杏鲍菇点云出现残缺问题,基于AdaPoinTr(Adaptive geometry-aware point transformers)提出了改进的SwinPoinTr模型,实现了对残缺杏鲍菇点云的准确补全和杏鲍菇表型参数的测量。该方法在使用提出的特征重塑模块的基础上,构建具有几何感知能力的层次化Transformer编码模块,提高了模型对输入点云的利用率和模型捕捉点云细节特征的能力。然后基于泊松重建方法完成了补全点云表面重建,并测量到杏鲍菇表型参数。实验结果表明,本文所提算法在残缺杏鲍菇点云补全任务中,模型倒角距离为1.316×10^(-4),地球移动距离为21.3282,F1分数为87.87%。在表型参数估测任务中,模型对杏鲍菇菌高、体积、表面积估测结果的决定系数分别为0.9582、0.9596、0.9605,均方根误差分别为4.4213 mm、10.8185 cm^(3)、7.5778 cm^(2)。结果证实了该研究方法可以有效地补全残缺的杏鲍菇点云,可以为菇房内杏鲍菇表型参数测量提供基础。展开更多
Based on the generalized bilinear method, diversity of exact solutions of the (3 + 1)-dimensional Kadomtsev-Petviashvili-Boussinesq-like equation is successfully derived by using symbolic computation with Maple. These...Based on the generalized bilinear method, diversity of exact solutions of the (3 + 1)-dimensional Kadomtsev-Petviashvili-Boussinesq-like equation is successfully derived by using symbolic computation with Maple. These new solutions, named three-wave solutions and periodic wave have greatly enriched the existing literature. Via the three-dimensional images, density images and contour plots, the physical characteristics of these waves are well described. The new three-wave solutions and periodic solitary wave solutions obtained in this paper, will have a wide range of applications in the fields of physics and mechanics.展开更多
We investigate the three-wave resonant interaction (TWRI) of Bogoliubov excitations in a disk-shaped Bose-Einstein condensate with the diffraction of the excitations taken into account. We show that the phase-matching...We investigate the three-wave resonant interaction (TWRI) of Bogoliubov excitations in a disk-shaped Bose-Einstein condensate with the diffraction of the excitations taken into account. We show that the phase-matching condition for the TWRI can be satisfied by a suitable selection of the wavevectors and the frequencies of the three exciting modes involved in the TWRI. Using a method of multiple-scales we derive a set of nonlinearly coupled envelope equations describing the TWRI process and give some explicit solitary-wave solutions.展开更多
The potential for nonlinear conversion between two laser pulses in a three-level V-type medium with assistance of an auxiliary microwave resonant radiation is studied. The results show that microwave driven field can ...The potential for nonlinear conversion between two laser pulses in a three-level V-type medium with assistance of an auxiliary microwave resonant radiation is studied. The results show that microwave driven field can lead to the parametric generation of a new laser pulse with high conversion efficieney when a weak pump laser pulse is applied.展开更多
In this paper. the coupling equations describing nonlinear three-wave interaction amongRossby waves including the forcing of an external vorticity source are obtained. Under certainconditions, the coupling equations w...In this paper. the coupling equations describing nonlinear three-wave interaction amongRossby waves including the forcing of an external vorticity source are obtained. Under certainconditions, the coupling equations with a constant amplitude forcing, the stability analysis indicates that when the amplitude of the external forcing increases to a certain extent, a pitchforkbifurcation occurs. Also. it is shown fi-o m numerical results that the bifurcation can lead to chaoticbehavior of' strange' attractor. For the obtained three-variable equation, when the amplitude ofmodulated external forcing gradually increases, a Period-doubling bifurcation is found to lead tochaotic behavior. Thus, in a nonlinear three-wave coupling model in the large-scale forcedbarotropic atmospheric flow, chaotic behavior can be observed. This chaotic behavior can explainin part 30-60-day low-flequency oscillations observed in mid-high latitudes.展开更多
基金supported by the Scientific and Technological Innovation Project of the China Academy of Chinese Medical Sciences(CI2021A04013)the National Natural Science Foundation of China(82204610)+1 种基金the Qihang Talent Program(L2022046)the Fundamental Research Funds for the Central Public Welfare Research Institutes(ZZ15-YQ-041 and L2021029).
文摘Background:The medicinal material known as Os Draconis(Longgu)originates from fossilized remains of ancient mammals and is widely used in treating emotional and mental conditions.However,fossil resources are nonrenewable,and clinical demand is increasingly difficult to meet,leading to a proliferation of counterfeit products.During prolonged geological burial,static pressure from the surrounding strata severely compromises the microstructural integrity of osteons in Os Draconis,but Os Draconis still largely retains the structural features of mammalian bone.Methods:Using verified authentic Os Draconis samples over 10,000 years old as a baseline,this study summarizes the ultrastructural characteristics of genuine Os Draconis.Employing electron probe microanalysis and optical polarized light microscopy,we examined 28 batches of authentic Os Draconis and 31 batches of counterfeits to identify their ultrastructural differences.Key points for ultrastructural identification of Os Draconis were compiled,and a new identification approach was proposed based on these differences.Results:Authentic Os Draconis exhibited distinct ultrastructural markers:irregularly shaped osteons with traversing fissures,deformed/displaced Haversian canals,and secondary mineral infill(predominantly calcium carbonate).Counterfeits showed regular osteon arrangements,absent traversal fissures,and homogeneous hydroxyapatite composition.Lab-simulated samples lacked structural degradation features.EPMA confirmed calcium carbonate infill in fossilized Haversian canals,while elemental profiles differentiated lacunae types(void vs.mineral-packed).Conclusion:The study established ultrastructural criteria for authentic Os Draconis identification:osteon deformation,geological fissures penetrating bone units,and heterogenous mineral deposition.These features,unattainable in counterfeits or modern processed bones,provide a cost-effective,accurate identification method.This approach bridges gaps in TCM material standardization and supports quality control for clinical applications.
基金supported by the National Natural Science Foundation of China(Grant Nos.52304139,52325403)the CCTEG Coal Mining Research Institute funding(Grant No.KCYJY-2024-MS-10).
文摘3D laser scanning technology is widely used in underground openings for high-precision,rapid,and nondestructive structural evaluations.Segmenting large 3D point cloud datasets,particularly in coal mine roadways with multi-scale targets,remains challenging.This paper proposes an enhanced segmentation method integrating improved PointNet++with a coverage-voted strategy.The coverage-voted strategy reduces data while preserving multi-scale target topology.The segmentation is achieved using an enhanced PointNet++algorithm with a normalization preprocessing head,resulting in a 94%accuracy for common supporting components.Ablation experiments show that the preprocessing head and coverage strategies increase segmentation accuracy by 20%and 2%,respectively,and improve Intersection over Union(IoU)for bearing plate segmentation by 58%and 20%.The accuracy of the current pretraining segmentation model may be affected by variations in surface support components,but it can be readily enhanced through re-optimization with additional labeled point cloud data.This proposed method,combined with a previously developed machine learning model that links rock bolt load and the deformation field of its bearing plate,provides a robust technique for simultaneously measuring the load of multiple rock bolts in a single laser scan.
基金Supported by the Shenzhen Science and Technology Program(No.JCYJ20240813152704006)the National Natural Science Foundation of China(No.62401259)+2 种基金the Fundamental Research Funds for the Central Universities(No.NZ2024036)the Postdoctoral Fellowship Program of CPSF(No.GZC20242228)High Performance Computing Platform of Nanjing University of Aeronautics and Astronautics。
文摘AIM:To construct an intelligent segmentation scheme for precise localization of central serous chorioretinopathy(CSC)leakage points,thereby enabling ophthalmologists to deliver accurate laser treatment without navigational laser equipment.METHODS:A dataset with dual labels(point-level and pixel-level)was first established based on fundus fluorescein angiography(FFA)images of CSC and subsequently divided into training(102 images),validation(40 images),and test(40 images)datasets.An intelligent segmentation method was then developed,based on the You Only Look Once version 8 Pose Estimation(YOLOv8-Pose)model and segment anything model(SAM),to segment CSC leakage points.Next,the YOLOv8-Pose model was trained for 200 epochs,and the best-performing model was selected to form the optimal combination with SAM.Additionally,the classic five types of U-Net series models[i.e.,U-Net,recurrent residual U-Net(R2U-Net),attention U-Net(AttU-Net),recurrent residual attention U-Net(R2AttUNet),and nested U-Net(UNet^(++))]were initialized with three random seeds and trained for 200 epochs,resulting in a total of 15 baseline models for comparison.Finally,based on the metrics including Dice similarity coefficient(DICE),intersection over union(IoU),precision,recall,precisionrecall(PR)curve,and receiver operating characteristic(ROC)curve,the proposed method was compared with baseline models through quantitative and qualitative experiments for leakage point segmentation,thereby demonstrating its effectiveness.RESULTS:With the increase of training epochs,the mAP50-95,Recall,and precision of the YOLOv8-Pose model showed a significant increase and tended to stabilize,and it achieved a preliminary localization success rate of 90%(i.e.,36 images)for CSC leakage points in 40 test images.Using manually expert-annotated pixel-level labels as the ground truth,the proposed method achieved outcomes with a DICE of 57.13%,an IoU of 45.31%,a precision of 45.91%,a recall of 93.57%,an area under the PR curve(AUC-PR)of 0.78 and an area under the ROC curve(AUC-ROC)of 0.97,which enables more accurate segmentation of CSC leakage points.CONCLUSION:By combining the precise localization capability of the YOLOv8-Pose model with the robust and flexible segmentation ability of SAM,the proposed method not only demonstrates the effectiveness of the YOLOv8-Pose model in detecting keypoint coordinates of CSC leakage points from the perspective of application innovation but also establishes a novel approach for accurate segmentation of CSC leakage points through the“detect-then-segment”strategy,thereby providing a potential auxiliary means for the automatic and precise realtime localization of leakage points during traditional laser photocoagulation for CSC.
基金financially supported by the National Key Research and Development Program of China(2021YFF0703600)。
文摘Highlights By conjugating the same anti-N monoclonal antibody(mAb4-mAb1)with colloidal gold or fluorescent microspheres,this study developed two rapid point-of-care antigen immunochromatographic strips for the detection of porcine deltacoronavirus.The fluorescent microsphere-based lateral flow test strip demonstrated a sensitivity of 10^(1.7)TCID_(50)/0.1 mL,which is fourfold higher than that of the colloidal gold-based assay.Porcine deltacoronavirus(PDCoV)is a recently identified enteric coronavirus that causes an acute infectious disease in piglets,leading to diarrhea,vomiting,dehydration,and mortality(Hu et al.2015).
文摘Based on the generalized bilinear method, diversity of exact solutions of the (3 + 1)-dimensional Kadomtsev-Petviashvili-Boussinesq-like equation is successfully derived by using symbolic computation with Maple. These new solutions, named three-wave solutions and periodic wave have greatly enriched the existing literature. Via the three-dimensional images, density images and contour plots, the physical characteristics of these waves are well described. The new three-wave solutions and periodic solitary wave solutions obtained in this paper, will have a wide range of applications in the fields of physics and mechanics.
文摘We investigate the three-wave resonant interaction (TWRI) of Bogoliubov excitations in a disk-shaped Bose-Einstein condensate with the diffraction of the excitations taken into account. We show that the phase-matching condition for the TWRI can be satisfied by a suitable selection of the wavevectors and the frequencies of the three exciting modes involved in the TWRI. Using a method of multiple-scales we derive a set of nonlinearly coupled envelope equations describing the TWRI process and give some explicit solitary-wave solutions.
基金Project supported in part by the National Natural Science Foundation of China （Grant Nos 60478029 and 10575040）.
文摘The potential for nonlinear conversion between two laser pulses in a three-level V-type medium with assistance of an auxiliary microwave resonant radiation is studied. The results show that microwave driven field can lead to the parametric generation of a new laser pulse with high conversion efficieney when a weak pump laser pulse is applied.
文摘In this paper. the coupling equations describing nonlinear three-wave interaction amongRossby waves including the forcing of an external vorticity source are obtained. Under certainconditions, the coupling equations with a constant amplitude forcing, the stability analysis indicates that when the amplitude of the external forcing increases to a certain extent, a pitchforkbifurcation occurs. Also. it is shown fi-o m numerical results that the bifurcation can lead to chaoticbehavior of' strange' attractor. For the obtained three-variable equation, when the amplitude ofmodulated external forcing gradually increases, a Period-doubling bifurcation is found to lead tochaotic behavior. Thus, in a nonlinear three-wave coupling model in the large-scale forcedbarotropic atmospheric flow, chaotic behavior can be observed. This chaotic behavior can explainin part 30-60-day low-flequency oscillations observed in mid-high latitudes.
