Three-dimensional(3D)culture systems are becoming increasingly popular due to their ability to mimic tissue-like structures more effectively than the monolayer cultures.In cancer and stem cell research,the natural cel...Three-dimensional(3D)culture systems are becoming increasingly popular due to their ability to mimic tissue-like structures more effectively than the monolayer cultures.In cancer and stem cell research,the natural cell characteristics and architectures are closely mimicked by the 3D cell models.Thus,the 3D cell cultures are promising and suitable systems for various proposes,ranging from disease modeling to drug target identification as well as potential therapeutic substances that may transform our lives.This review provides a comprehensive compendium of recent advancements in culturing cells,in particular cancer and stem cells,using 3D culture techniques.The major approaches highlighted here include cell spheroids,hydrogel embedding,bioreactors,scaffolds,and bioprinting.In addition,the progress of employing 3D cell culture systems as a platform for cancer and stem cell research was addressed,and the prominent studies of 3D cell culture systems were discussed.展开更多
With the swift development of China’s soft power and the Belt and Road initiative,the cultural interaction between China and the West become more and more frequent.As an art,the film plays a vital role in spreading C...With the swift development of China’s soft power and the Belt and Road initiative,the cultural interaction between China and the West become more and more frequent.As an art,the film plays a vital role in spreading Chinese culture,and meantime,the film also is a significant means of cross-cultural communication,while the movie subtitle is a carrier to convey the film content and inner spirit to the foreigners.The study analyses the movie subtitle translation of Changjin Lake from three-dimensional selection adaptive conversion perspectives through comparing its source target to its translation target to leave a deeper understanding of translation skills and inner Chinese culture,which will further promote the“going out”of Chinese culture.展开更多
Cancer stem cells(CSCs)are a relatively rare subpopulation of tumor cell with self-renewal and tumorigenesis capabilities.CSCs are associated with cancer recurrence,progression,and chemoradiotherapy resistance.Establi...Cancer stem cells(CSCs)are a relatively rare subpopulation of tumor cell with self-renewal and tumorigenesis capabilities.CSCs are associated with cancer recurrence,progression,and chemoradiotherapy resistance.Establishing a reliable platform for CSC enrichment and study is a prerequisite for understanding the characteristics of CSCs and discovering CSC-related therapeutic strategies.Certain strategies for CSC enrichment have been used in laboratory,particularly fluorescence-activated cell sorting(FACS)and mammosphere culture.However,these methods fail to recapitulate the in vivo chemical and physical conditions in tumors,thus potentially decreasing the malignancy of CSCs in culture and yielding unreliable research results.Accumulating research suggests the promise of a biomaterial-based three-dimensional(3 D)strategy for CSC enrichment and study.This strategy has an advantage over conventional methods in simulating the tumor microenvironment,thus providing a more effective and predictive model for CSC laboratory research.In this review,we first briefly discuss the conventional methods for CSC enrichment and study.We then summarize the latest advances and challenges in biomaterial-based 3 D CSC platforms.Design strategies for materials,morphology,and chemical and physical cues are highlighted to provide direction for the future construction of platforms for CSC enrichment and study.展开更多
This article conducts an analysis and exploration of the English subtitles translation of the domestic animated film Chang’an from the perspective of eco-translatology.Taking three-dimensional transformation as the e...This article conducts an analysis and exploration of the English subtitles translation of the domestic animated film Chang’an from the perspective of eco-translatology.Taking three-dimensional transformation as the entry point,it analyzes the adaptation and selection of subtitle translators in the translation process from the dimensions of language,culture,and communication in Chang’an,aiming to enrich the practical analysis of eco-translatology and provide valuable insights and references for the theoretical construction and practical application of eco-translatology.This article enriches the practical analysis of eco-translatology and also validates to a certain extent the applicability of“three-dimensional”transformation.展开更多
Eco-translatology provides a new perspective and methodology for the international publicity translation of political texts.This paper applies the viewpoint and methodology of eco-translatology,focuses on the three-di...Eco-translatology provides a new perspective and methodology for the international publicity translation of political texts.This paper applies the viewpoint and methodology of eco-translatology,focuses on the three-dimensional transformation of language,culture,and communication,and discusses how translators can adapt to the eco-environment of political texts through the specific example of the keynote speech of China’s president at the opening ceremony of the Third Belt and Road Forum for International Cooperation and select suitable translation strategies and techniques to achieve an ecological balance of the target text in multiple dimensions.展开更多
文摘Three-dimensional(3D)culture systems are becoming increasingly popular due to their ability to mimic tissue-like structures more effectively than the monolayer cultures.In cancer and stem cell research,the natural cell characteristics and architectures are closely mimicked by the 3D cell models.Thus,the 3D cell cultures are promising and suitable systems for various proposes,ranging from disease modeling to drug target identification as well as potential therapeutic substances that may transform our lives.This review provides a comprehensive compendium of recent advancements in culturing cells,in particular cancer and stem cells,using 3D culture techniques.The major approaches highlighted here include cell spheroids,hydrogel embedding,bioreactors,scaffolds,and bioprinting.In addition,the progress of employing 3D cell culture systems as a platform for cancer and stem cell research was addressed,and the prominent studies of 3D cell culture systems were discussed.
文摘With the swift development of China’s soft power and the Belt and Road initiative,the cultural interaction between China and the West become more and more frequent.As an art,the film plays a vital role in spreading Chinese culture,and meantime,the film also is a significant means of cross-cultural communication,while the movie subtitle is a carrier to convey the film content and inner spirit to the foreigners.The study analyses the movie subtitle translation of Changjin Lake from three-dimensional selection adaptive conversion perspectives through comparing its source target to its translation target to leave a deeper understanding of translation skills and inner Chinese culture,which will further promote the“going out”of Chinese culture.
基金supported by grants from the National Natural Science Foundation of China(Grant Nos.11832008 and 81703012)the Program of the Postgraduate Tutor Team of Chongqing Education Commission(2018)+1 种基金the Fundamental Research Funds for the Central Universities(Grant No.2019CDXYSG0004)the Visiting Scholar Foundation of the Key Laboratory of Biorheological Science and Technology(Chongqing University),Ministry of Education(Grant No.CQKLBST-2017-005)。
文摘Cancer stem cells(CSCs)are a relatively rare subpopulation of tumor cell with self-renewal and tumorigenesis capabilities.CSCs are associated with cancer recurrence,progression,and chemoradiotherapy resistance.Establishing a reliable platform for CSC enrichment and study is a prerequisite for understanding the characteristics of CSCs and discovering CSC-related therapeutic strategies.Certain strategies for CSC enrichment have been used in laboratory,particularly fluorescence-activated cell sorting(FACS)and mammosphere culture.However,these methods fail to recapitulate the in vivo chemical and physical conditions in tumors,thus potentially decreasing the malignancy of CSCs in culture and yielding unreliable research results.Accumulating research suggests the promise of a biomaterial-based three-dimensional(3 D)strategy for CSC enrichment and study.This strategy has an advantage over conventional methods in simulating the tumor microenvironment,thus providing a more effective and predictive model for CSC laboratory research.In this review,we first briefly discuss the conventional methods for CSC enrichment and study.We then summarize the latest advances and challenges in biomaterial-based 3 D CSC platforms.Design strategies for materials,morphology,and chemical and physical cues are highlighted to provide direction for the future construction of platforms for CSC enrichment and study.
文摘This article conducts an analysis and exploration of the English subtitles translation of the domestic animated film Chang’an from the perspective of eco-translatology.Taking three-dimensional transformation as the entry point,it analyzes the adaptation and selection of subtitle translators in the translation process from the dimensions of language,culture,and communication in Chang’an,aiming to enrich the practical analysis of eco-translatology and provide valuable insights and references for the theoretical construction and practical application of eco-translatology.This article enriches the practical analysis of eco-translatology and also validates to a certain extent the applicability of“three-dimensional”transformation.
文摘Eco-translatology provides a new perspective and methodology for the international publicity translation of political texts.This paper applies the viewpoint and methodology of eco-translatology,focuses on the three-dimensional transformation of language,culture,and communication,and discusses how translators can adapt to the eco-environment of political texts through the specific example of the keynote speech of China’s president at the opening ceremony of the Third Belt and Road Forum for International Cooperation and select suitable translation strategies and techniques to achieve an ecological balance of the target text in multiple dimensions.
