A 3-D unstructured-grid, finite-volume coastal ocean model （FVCOM） was used to study the tides and tidal currents in the Lianzhou Bay and adjacent areas. The simulation results were in good agreement with observatio...A 3-D unstructured-grid, finite-volume coastal ocean model （FVCOM） was used to study the tides and tidal currents in the Lianzhou Bay and adjacent areas. The simulation results were in good agreement with observations. The co-tidal maps of K1 , O1 , and M2 indicated that K1 and O1 were mostly standing waves outside the Lianzhou Bay, while M2 had a largely propagating character. However, all three constituents became propagating waves when entering the Lianzhou Bay, due to the shallow waters. The tidal current ellipses showed the characters of K1 , O1 , and M2 constituents： K1 and O1 were rotating outside the bay, but rectilinear along the water channels inside the bay; M2 was mostly rectilinear over the whole area. The tidal-induced residual current shows the flow was divided into two branches by the Guantouling Peninsula： one turned to flow west; the other was blocked by the southern boundary of the peninsula, creating a clockwise circulation. In Lianzhou Bay, there were two circulation systems, a cyclonic one at the top of the bay and an anti-cyclonic at the mouth.展开更多
The characteristics of three-dimensional （3-D） tidal current in the Oujiang Estuary are investigated according to in situ observations. The Oujiang Estuary has features of irregular coastline, complex topography, ma...The characteristics of three-dimensional （3-D） tidal current in the Oujiang Estuary are investigated according to in situ observations. The Oujiang Estuary has features of irregular coastline, complex topography, many islands, moveable boundary, and submerged dyke, therefore, σ 3-D numerical model oil an unstructured triangular grid has been degeloped. The σ coordinate transforination, the moveable boundary and submerged dyke treatment techniques were employed in the model so it is suitable for the tidal simulations in the Oujing Estuary with submerged dyke and moveable boundary problems. The model is evaluated with the in situ data, and the results show that the calculated water elevations at 19 stations and currents at 19 profiler stations are in good agreement with measured data both in magnitude and phase. This numerical model is applied to the 3-D tidal circulation simulations of experiments in stopping flow transport through the South Branch of the Oujiang Estuary, and the feasibility to cutoff the flow in the South Branch of the Oujiang Estuary is demonstrated by numerical simulation experiments. The developed numerical model simulated the 3-D tidal current circulations in complicated coastal and estuarine waters very well.展开更多
By means of three dimensional POM model,which computes the eddy viscosity coefficient based on two order turbulent closed model,the tide and tidal current in the Beibu Gulf are simulated with fine grid.In the comput...By means of three dimensional POM model,which computes the eddy viscosity coefficient based on two order turbulent closed model,the tide and tidal current in the Beibu Gulf are simulated with fine grid.In the computed region,six islands are considered and the bottom friction coefficients are taken to be various values from the difference of sea region.Acquired tidal harmonic constants are compared with that of 81 tidal observatories.The absolute mean error of K 1 constituent amplitude is 4 6 cm and of the lag is 9°.The absolute mean error of O 1 constituent amplitude is 5 6 cm and of the lag is 7°.The absolute mean error of M 2 constituent amplitude is 6 2 cm and of the lag is 15°.The tide,tidal current,residual water level and tide induced residual current,as well as the vertial distribution of horizontal velocity in this sea region are analysed by the simulated results.展开更多
With the continuous development of digital medicine,minimally invasive precision and safety have become the primary development trends in hepatobiliary surgery.Due to the specificity and complexity of hepatobiliary su...With the continuous development of digital medicine,minimally invasive precision and safety have become the primary development trends in hepatobiliary surgery.Due to the specificity and complexity of hepatobiliary surgery,traditional preoperative imaging techniques such as computed tomography and magnetic resonance imaging cannot meet the need for identification of fine anatomical regions.Imaging-based three-dimensional(3D)reconstruction,virtual simulation of surgery and 3D printing optimize the surgical plan through preoperative assessment,improving the controllability and safety of intraoperative operations,and in difficult-to-reach areas of the posterior and superior liver,assistive robots reproduce the surgeon’s natural movements with stable cameras,reducing natural vibrations.Electromagnetic navigation in abdominal surgery solves the problem of conventional surgery still relying on direct visual observation or preoperative image assessment.We summarize and compare these recent trends in digital medical solutions for the future development and refinement of digital medicine in hepatobiliary surgery.展开更多
Graphite anode materials are widely used in commercial lithium-ion batteries;however, the long electron/ion transportation path restricted its high energy storage. In this experiment, we designed a copper/graphite com...Graphite anode materials are widely used in commercial lithium-ion batteries;however, the long electron/ion transportation path restricted its high energy storage. In this experiment, we designed a copper/graphite composite with a dual three-dimensional(3 D) continuous porous structure combining used nonsolvent-induced phase separation and heat treatment, in which a large amount of graphite is embedded in the 3 D porous copper/carbon architecture. In the novel structure, not only the electron and Li^(+) transmission performances are improved, but also the space of current collector is fully utilized. Meanwhile,carbonized polyacrylonitrile network stabilizes the interface between graphite and copper matrix. The obtained copper/graphite composite anode has an initial discharge capacity of 524.6 mAh·g^(-1), a holding capacity of350 mAh·g^(-1) and excellent cycle stability(299.3 mAh·g^(-1) after 180 cycles at 0.1 C rate), exhibiting good electrochemical performance. The experimental results show that the mass loading of the copper/graphite composite electrode material is about 4.39 mg·cm^(-2). We also envisage replacing graphite with other high-capacity active materials to fill the current collector, which can provide a reference for the future development of next-generation advanced electrodes.展开更多
On the basis of a three-dimensional weakly nonliear theory of Lagrangian residual current in the Baroclinic shallow seas, a diagnostic numerical calculation of wind-driven, thermohaline and tide-induced Lagrangian res...On the basis of a three-dimensional weakly nonliear theory of Lagrangian residual current in the Baroclinic shallow seas, a diagnostic numerical calculation of wind-driven, thermohaline and tide-induced Lagrangian residual current in the Bohai Sea is made. The model involves the Richardson number in the eddy viscosity coefficient, wind, thcrmolialine and tidal effects in the focing terms. The runoff of the Huanghe River and a part of the Huanghai Warm Water coming from the Huanghai Sea through the Bohai Sea Strait is also considered. The velocity-splitting method is adopted. The wind-driven circu lation, thermohaline circulation and the tide-induced Lagrangian residual circulation are also obtained individually and analysed. The dynamics of the three main eddies in the Lagrangian mean circulation is discussed. Finally, the numerical result is partly verified with the observed data.展开更多
The model equations with tbree-dimensional, time-dependent, nonlinear Navier-Stokes equations are transformed by sigma-transformation.On the basis of the process splitting technique, the fluid flow problems are divid...The model equations with tbree-dimensional, time-dependent, nonlinear Navier-Stokes equations are transformed by sigma-transformation.On the basis of the process splitting technique, the fluid flow problems are divided into two parts:the vertically-intopated equations (external mode) and the vertical structure equations(internal mace). The first set of equations being the propagation of the tidal weves and the ADI numerical scheme has ben chosen to solve them. Conerning the vertical structure equations, they are solved by means of leapfrog stepping procedure.The main features of the tide and associated tidal current in the Bohai Sea are examined with this 3-D model.To have a good reproduction of vertical structure, the column is divided into 10 layers and the M2 tidal current is computed in detail. The simulation reveal the spetial structure and some important characteristics of the tidal current of the Bohai Sea. The application of the 3-D madel to forecasting of the tidal current in the Bobal Sea has been Performed as an illustration.展开更多
Three-dimensional(3D)urban structures play a critical role in informing climate mitigation strategies aimed at the built environment and facilitating sustainable urban development.Regrettably,there exists a significan...Three-dimensional(3D)urban structures play a critical role in informing climate mitigation strategies aimed at the built environment and facilitating sustainable urban development.Regrettably,there exists a significant gap in detailed and consistent data on 3D building space structures with global coverage due to the challenges inherent in the data collection and model calibration processes.In this study,we constructed a global urban structure(GUS-3D)dataset,including building volume,height,and footprint information,at a 500 m spatial resolution using extensive satellite observation products and numerous reference building samples.Our analysis indicated that the total volume of buildings worldwide in2015 exceeded 1×10^(12)m^(3).Over the 1985 to 2015 period,we observed a slight increase in the magnitude of 3D building volume growth(i.e.,it increased from 166.02 km3 during the 1985–2000 period to 175.08km3 during the 2000–2015 period),while the expansion magnitudes of the two-dimensional(2D)building footprint(22.51×10^(3) vs 13.29×10^(3)km^(2))and urban extent(157×10^(3) vs 133.8×10^(3)km^(2))notably decreased.This trend highlights the significant increase in intensive vertical utilization of urban land.Furthermore,we identified significant heterogeneity in building space provision and inequality across cities worldwide.This inequality is particularly pronounced in many populous Asian cities,which has been overlooked in previous studies on economic inequality.The GUS-3D dataset shows great potential to deepen our understanding of the urban environment and creates new horizons for numerous 3D urban studies.展开更多
BACKGROUND Three-dimensional(3D)modelling technology translates the patient-specific anatomical information derived from two-dimensional radiological images into virtual or physical 3D models,which more closely resemb...BACKGROUND Three-dimensional(3D)modelling technology translates the patient-specific anatomical information derived from two-dimensional radiological images into virtual or physical 3D models,which more closely resemble the complex environment encountered during surgery.It has been successfully applied to surgical planning and navigation,as well as surgical training and patient education in several surgical specialties,but its uptake lags behind in colorectal surgery.Rectal cancer surgery poses specific challenges due to the complex anatomy of the pelvis,which is difficult to comprehend and visualise.AIM To review the current and emerging applications of the 3D models,both virtual and physical,in rectal cancer surgery。METHODS Medline/PubMed,Embase and Scopus databases were searched using the keywords“rectal surgery”,“colorectal surgery”,“three-dimensional”,“3D”,“modelling”,“3D printing”,“surgical planning”,“surgical navigation”,“surgical education”,“patient education”to identify the eligible full-text studies published in English between 2001 and 2020.Reference list from each article was manually reviewed to identify additional relevant papers.The conference abstracts,animal and cadaveric studies and studies describing 3D pelvimetry or radiotherapy planning were excluded.Data were extracted from the retrieved manuscripts and summarised in a descriptive way.The manuscript was prepared and revised in accordance with PRISMA 2009 checklist.RESULTS Sixteen studies,including 9 feasibility studies,were included in the systematic review.The studies were classified into four categories:feasibility of the use of 3D modelling technology in rectal cancer surgery,preoperative planning and intraoperative navigation,surgical education and surgical device design.Thirteen studies used virtual models,one 3D printed model and 2 both types of models.The construction of virtual and physical models depicting the normal pelvic anatomy and rectal cancer,was shown to be feasible.Within the clinical context,3D models were used to identify vascular anomalies,for surgical planning and navigation in lateral pelvic wall lymph node dissection and in management of recurrent rectal cancer.Both physical and virtual 3D models were found to be valuable in surgical education,with a preference for 3D printed models.The main limitations of the current technology identified in the studies were related to the restrictions of the segmentation process and the lack of 3D printing materials that could mimic the soft and deformable tissues.CONCLUSION 3D modelling technology has potential to be utilised in multiple aspects of rectal cancer surgery,however,it is still at the experimental stage of application in this setting.展开更多
BACKGROUND Inguinal hernias are common after surgery.Tension-free repair is widely accepted as the main method for managing inguinal hernias.Adequate exposure,coverage,and repair of the myopectineal orifice(MPO)are ne...BACKGROUND Inguinal hernias are common after surgery.Tension-free repair is widely accepted as the main method for managing inguinal hernias.Adequate exposure,coverage,and repair of the myopectineal orifice(MPO)are necessary.However,due to differences in race and sex,people’s body shapes vary.According to European guidelines,the patch should measure 10 cm×15 cm.If any part of the MPO is dissected,injury to the nerves,vascular network,or organs may occur during surgery,thereby leading to inguinal discomfort,pain,and seroma formation after surgery.Therefore,accurate localization and measurement of the boundary of the MPO are crucial for selecting the optimal patch for inguinal hernia repair.AIM To compare the size of the MPO measured on three-dimensional multislice spiral computed tomography(CT)with that measured via laparoscopy and explore the relevant factors influencing the size of the MPO.METHODS Clinical data from 74 patients who underwent laparoscopic tension-free inguinal hernia repair at the General Surgery Department of the First Affiliated Hospital of Anhui University of Science and Technology between September 2022 and July 2024 were collected and analyzed retrospectively.Transabdominal preperitoneal was performed.Sixty-four males and 10 females,with an average age of 58.30±12.32 years,were included.The clinical data of the patients were collected.The boundary of the MPO was measured on three-dimensional CT images before surgery and then again during transabdominal preperitoneal.All the preoperative and intraoperative data were analyzed via paired t-tests.A t-test was used for comparisons of age,body mass index,and sex between the groups.In the comparative analysis,a P value less than 0.05 indicated a significant difference.RESULTS The boundaries of the MPO on 3-dimensional CT images measured 7.05±0.47 cm and 6.27±0.61 cm,and the area of the MPO was 19.54±3.33 cm^(2).The boundaries of the MPO during surgery were 7.18±0.51 cm and 6.17±0.40 cm.The errors were not statistically significant.However,the intraoperative BD(the width of the MPO,P=0.024,P<0.05)and preoperative AC(the length of the MPO,P=0.045,P<0.05)significantly differed according to sex.The AC and BD measurements before and during surgery were not significantly different according to age,body mass index,hernia side or hernia type(P>0.05).CONCLUSION The application of this technology can aid in determining the most appropriate dissection range and patch size.展开更多
To address the problem of multi-missile cooperative interception against maneuvering targets at a prespecified impact time and desired Line-of-Sight(LOS)angles in ThreeDimensional(3D)space,this paper proposes a 3D lea...To address the problem of multi-missile cooperative interception against maneuvering targets at a prespecified impact time and desired Line-of-Sight(LOS)angles in ThreeDimensional(3D)space,this paper proposes a 3D leader-following cooperative interception guidance law.First,in the LOS direction of the leader,an impact time-controlled guidance law is derived based on the fixed-time stability theory,which enables the leader to complete the interception task at a prespecified impact time.Next,in the LOS direction of the followers,by introducing a time consensus tracking error function,a fixed-time consensus tracking guidance law is investigated to guarantee the consensus tracking convergence of the time-to-go.Then,in the direction normal to the LOS,by combining the designed global integral sliding mode surface and the second-order Sliding Mode Control(SMC)theory,an innovative 3D LOS-angle-constrained interception guidance law is developed,which eliminates the reaching phase in the traditional sliding mode guidance laws and effectively saves energy consumption.Moreover,it effectively suppresses the chattering phenomenon while avoiding the singularity issue,and compensates for unknown interference caused by target maneuvering online,making it convenient for practical engineering applications.Finally,theoretical proof analysis and multiple sets of numerical simulation results verify the effectiveness,superiority,and robustness of the investigated guidance law.展开更多
A three-dimensional wave radiation stress is introduced into the hydrodynamic sediment coupled model COHERENS-SED, which has been developed through introducing wave-enhanced bottom shear stress, wave dependent surface...A three-dimensional wave radiation stress is introduced into the hydrodynamic sediment coupled model COHERENS-SED, which has been developed through introducing wave-enhanced bottom shear stress, wave dependent surface drag coefficient, wave-induced surface mixing, SWAN, damping function of sediment on turbulence, sediment model and depth-dependent wave radiation stress to COHERENS. The COHERENS-SED is adopted to study the effects induced by wave-induced three-dimensional longshore current on suspended sediment spreading of the Huanghe River （Yellow River） mouth. Several different cases divided by setting different wave parameters of inputting boundary waves are carried out. The modeling results agree with measurement data. In terms of simulation results, it is easy to know that three-dimensional wave radiation stress plays an obvious role when inputting boundary wave height is stronger than 3 m. Moreover, wave direction also affects the sediment spreading rules of the mouth strongly too.展开更多
The semidiagnostic and prognostic models are used to compute the current in the Taiwan Strait with wind and hydrographic data collected during August, 1984 and September 1 ~ 6, 1988. This calculation can be divided in...The semidiagnostic and prognostic models are used to compute the current in the Taiwan Strait with wind and hydrographic data collected during August, 1984 and September 1 ~ 6, 1988. This calculation can be divided into two stages, i. e. (1 ) the adjustable stage; (2) prognostic calculation. The computed result shows tha the density and velocity fields etc. have been adjusted when t = 2. 5 d, namely the solution of semidignostic calculation is obtained,and the quasi-steady state solution have been reached after about 40 d. Comparing the results of diagncotic calculation with those of semidiagnortic and prognostic calculations indicates that they agree qualitatively. For example, they all have the following common features: (1 ) there is a persistent northward fiow with a volume transport of 0. 8 × 10 6 m3/s through the Taiwan Strait in summer; (2 ) the current near the western coas of Taiwan is stronger than that in other regiona; (3) the upweiling occurs near the Fujian coast and so on. However, there is a quantitative difference between them as follows. For example, the horizotal velocity near the westem cot of Taiwan and the upwelling speed near the Fujian aret both are underestimaed in the diagnestic calculation, because the data used in which is smoothed, and they both are intensified in the solutions of semidiagnostic and prognostic calculations. For example, the maximum velocity near the western coast of Taiwan at t = 0 d (diagnostic), 2. 5 d (semidiagnostic) and 300 d (prognostic) is 59.1, 62. 1 and 62. 0 cm/s, respectively. From the above comparison we see it is quite necessary that a semidiagnostic model be used to compute the currents when the data have been smoothed.展开更多
By coupling the three-dimensional hydrodynamic model with the wave model, numerical simulations of the three- dimensional wave-induced current are carried out in this study. The wave model is based on the numerical so...By coupling the three-dimensional hydrodynamic model with the wave model, numerical simulations of the three- dimensional wave-induced current are carried out in this study. The wave model is based on the numerical solution of the modified wave action equation and eikonal equation, which can describe the wave refraction and diffraction. The hydrodynamic model is driven by the wave-induced radiation stresses and affected by the wave turbulence. The numerical implementation of the module has used the finite-volume schemes on unstructured grid, which provides great flexibility for modeling the waves and currents in the complex actual nearshore, and ensures the conservation of energy propagation. The applicability of the proposed model is evaluated in calculating the cases of wave set-up, longshore currents, undertow on a sloping beach, rip currents and meandering longshore currents on a tri-cuspate beach. The results indicate that it is necessary to introduce the depth-dependent radiation stresses into the numerical simulation of wave-induced currents, and comparisons show that the present model makes better prediction on the wave procedure as well as both horizontal and vertical structures in the wave-induced current field.展开更多
Based on the wind and hydrographic data obtained by R/V Xiangyanghong 14 during June of 1999, the currents in the Huanghai Sea and East China Sea are computed by the three dimensional non-linear diagnostic, semidiagno...Based on the wind and hydrographic data obtained by R/V Xiangyanghong 14 during June of 1999, the currents in the Huanghai Sea and East China Sea are computed by the three dimensional non-linear diagnostic, semidiagnostic models and prognostic in the a coordinate. The computed results show that the density and velocity fields and so on have been adjusted when time is about 3 days, namely the solution of semidiagnostic calculation is obtained. In the northwest part of the computed region, the Huanghai coastal current flows southeastward, and then it flows out the computed region south of Cheju Island. In the west side of the southern part of the computed region, there is other current, which is mainly inshore branch of Taiwan Warm Current, and it flows cyclonically and turns to the northeast. In the region north of the above two currents, there is a cyclonic eddy southwest of Cheju Island, and it has characteristics of high density and low temperature. There is an offshore branch of Taiwan Warm Current in the west side of the Kuroshio, and it makes a cyclonic meander, then flows northeastward. The Kuroshio in the East China Sea is stronger, and flows northeastward. Its maximum horizontal velocity is 108.5 cm/s at the sea surface, which is located at the northern boundary, and it is 106.1 cm/s at 30 m level, 102.2 cm/s at 75 m level and 85.1 cm/s at 200 m level, respectively, which are all located at the southern boundary. Comparing the results of diagnostic calculation with those of semidiagnostic and prognostic calculations indicates that the horizontal velocity field agrees qualitatively, and there is a little difference between them in quantity. The comparison between the computed velocities and the observed velocities at the mooring station show that they agree each other.展开更多
It is of great importance to obtain precise trace data,as traces are frequently the sole visible and measurable parameter in most outcrops.The manual recognition and detection of traces on high-resolution three-dimens...It is of great importance to obtain precise trace data,as traces are frequently the sole visible and measurable parameter in most outcrops.The manual recognition and detection of traces on high-resolution three-dimensional(3D)models are relatively straightforward but time-consuming.One potential solution to enhance this process is to use machine learning algorithms to detect the 3D traces.In this study,a unique pixel-wise texture mapper algorithm generates a dense point cloud representation of an outcrop with the precise resolution of the original textured 3D model.A virtual digital image rendering was then employed to capture virtual images of selected regions.This technique helps to overcome limitations caused by the surface morphology of the rock mass,such as restricted access,lighting conditions,and shading effects.After AI-powered trace detection on two-dimensional(2D)images,a 3D data structuring technique was applied to the selected trace pixels.In the 3D data structuring,the trace data were structured through 2D thinning,3D reprojection,clustering,segmentation,and segment linking.Finally,the linked segments were exported as 3D polylines,with each polyline in the output corresponding to a trace.The efficacy of the proposed method was assessed using a 3D model of a real-world case study,which was used to compare the results of artificial intelligence(AI)-aided and human intelligence trace detection.Rosette diagrams,which visualize the distribution of trace orientations,confirmed the high similarity between the automatically and manually generated trace maps.In conclusion,the proposed semi-automatic method was easy to use,fast,and accurate in detecting the dominant jointing system of the rock mass.展开更多
Currently,there are a limited number of dynamic models available for braided composite plates with large overall motions,despite the incorporation of three-dimensional(3D)braided composites into rotating blade compone...Currently,there are a limited number of dynamic models available for braided composite plates with large overall motions,despite the incorporation of three-dimensional(3D)braided composites into rotating blade components.In this paper,a dynamic model of 3D 4-directional braided composite thin plates considering braiding directions is established.Based on Kirchhoff's plate assumptions,the displacement variables of the plate are expressed.By incorporating the braiding directions into the constitutive equation of the braided composites,the dynamic model of the plate considering braiding directions is obtained.The effects of the speeds,braiding directions,and braided angles on the responses of the plate with fixed-axis rotation and translational motion,respectively,are investigated.This paper presents a dynamic theory for calculating the deformation of 3D braided composite structures undergoing both translational and rotational motions.It also provides a simulation method for investigating the dynamic behavior of non-isotropic material plates in various applications.展开更多
Liposarcoma is one of the most common soft tissue sarcomas,however,its occurrence rate is still rare compared to other cancers.Due to its rarity,in vitro experiments are an essential approach to elucidate liposarcoma ...Liposarcoma is one of the most common soft tissue sarcomas,however,its occurrence rate is still rare compared to other cancers.Due to its rarity,in vitro experiments are an essential approach to elucidate liposarcoma pathobiology.Conventional cell culture-based research(2D cell culture)is still playing a pivotal role,while several shortcomings have been recently under discussion.In vivo,mouse models are usually adopted for pre-clinical analyses with expectations to overcome the issues of 2D cell culture.However,they do not fully recapitulate human dedifferentiated liposarcoma(DDLPS)characteristics.Therefore,three-dimensional(3D)culture systems have been the recent research focus in the cell biology field with the expectation to overcome at the same time the disadvantages of 2D cell culture and in vivo animal models and fill in the gap between them.Given the liposarcoma rarity,we believe that 3D cell culture techniques,including 3D cell cultures/co-cultures,and Patient-Derived tumor Organoids(PDOs),represent a promising approach to facilitate liposarcoma investigation and elucidate its molecular mechanisms and effective therapy development.In this review,we first provide a general overview of 3D cell cultures compared to 2D cell cultures.We then focus on one of the recent 3D cell culture applications,Patient-Derived Organoids(PDOs),summarizing and discussing several PDO methodologies.Finally,we discuss the current and future applications of PDOs to sarcoma,particularly in the field of liposarcoma.展开更多
Bone repair remains an important target in tissue engineering,making the development of bioactive scaffolds for effective bone defect repair a critical objective.In this study,β-tricalcium phosphate(β-TCP)scaffolds ...Bone repair remains an important target in tissue engineering,making the development of bioactive scaffolds for effective bone defect repair a critical objective.In this study,β-tricalcium phosphate(β-TCP)scaffolds incorporated with processed pyritum decoction(PPD)were fabricated using three-dimensional(3D)printing-assisted freeze-casting.The produced composite scaffolds were evaluated for their mechanical strength,physicochemical properties,biocompatibility,in vitro proangiogenic activity,and in vivo efficacy in repairing rabbit femoral defects.They not only demonstrated excellent physicochemical properties,enhanced mechanical strength,and good biosafety but also significantly promoted the proliferation,migration,and aggregation of pro-angiogenic human umbilical vein endothelial cells(HUVECs).In vivo studies revealed that all scaffold groups facilitated osteogenesis at the bone defect site,with theβ-TCP scaffolds loaded with PPD markedly enhancing the expression of neurogenic locus Notch homolog protein 1(Notch1),vascular endothelial growth factor(VEGF),bone morphogenetic protein-2(BMP-2),and osteopontin(OPN).Overall,the scaffolds developed in this study exhibited strong angiogenic and osteogenic capabilities both in vitro and in vivo.The incorporation of PPD notably promoted the angiogenic-osteogenic coupling,thereby accelerating bone repair,which suggests that PPD is a promising material for bone repair and that the PPD/β-TCP scaffolds hold great potential as a bone graft alternative.展开更多
基金supported by the special fund for the Ocean Public Welfare Scientific Research Project, State Oceanic Administration, People's Republic of China(Grant No. 200805065)
文摘A 3-D unstructured-grid, finite-volume coastal ocean model （FVCOM） was used to study the tides and tidal currents in the Lianzhou Bay and adjacent areas. The simulation results were in good agreement with observations. The co-tidal maps of K1 , O1 , and M2 indicated that K1 and O1 were mostly standing waves outside the Lianzhou Bay, while M2 had a largely propagating character. However, all three constituents became propagating waves when entering the Lianzhou Bay, due to the shallow waters. The tidal current ellipses showed the characters of K1 , O1 , and M2 constituents： K1 and O1 were rotating outside the bay, but rectilinear along the water channels inside the bay; M2 was mostly rectilinear over the whole area. The tidal-induced residual current shows the flow was divided into two branches by the Guantouling Peninsula： one turned to flow west; the other was blocked by the southern boundary of the peninsula, creating a clockwise circulation. In Lianzhou Bay, there were two circulation systems, a cyclonic one at the top of the bay and an anti-cyclonic at the mouth.
基金The Natural Science Foundation of Tianjin, China under contract No.08JCZDZT00200
文摘The characteristics of three-dimensional （3-D） tidal current in the Oujiang Estuary are investigated according to in situ observations. The Oujiang Estuary has features of irregular coastline, complex topography, many islands, moveable boundary, and submerged dyke, therefore, σ 3-D numerical model oil an unstructured triangular grid has been degeloped. The σ coordinate transforination, the moveable boundary and submerged dyke treatment techniques were employed in the model so it is suitable for the tidal simulations in the Oujing Estuary with submerged dyke and moveable boundary problems. The model is evaluated with the in situ data, and the results show that the calculated water elevations at 19 stations and currents at 19 profiler stations are in good agreement with measured data both in magnitude and phase. This numerical model is applied to the 3-D tidal circulation simulations of experiments in stopping flow transport through the South Branch of the Oujiang Estuary, and the feasibility to cutoff the flow in the South Branch of the Oujiang Estuary is demonstrated by numerical simulation experiments. The developed numerical model simulated the 3-D tidal current circulations in complicated coastal and estuarine waters very well.
文摘By means of three dimensional POM model,which computes the eddy viscosity coefficient based on two order turbulent closed model,the tide and tidal current in the Beibu Gulf are simulated with fine grid.In the computed region,six islands are considered and the bottom friction coefficients are taken to be various values from the difference of sea region.Acquired tidal harmonic constants are compared with that of 81 tidal observatories.The absolute mean error of K 1 constituent amplitude is 4 6 cm and of the lag is 9°.The absolute mean error of O 1 constituent amplitude is 5 6 cm and of the lag is 7°.The absolute mean error of M 2 constituent amplitude is 6 2 cm and of the lag is 15°.The tide,tidal current,residual water level and tide induced residual current,as well as the vertial distribution of horizontal velocity in this sea region are analysed by the simulated results.
基金Supported by National Natural Science Foundation of China,No.82070638 and No.81770621and JSPS KAKENHI,No.JP18H02866.
文摘With the continuous development of digital medicine,minimally invasive precision and safety have become the primary development trends in hepatobiliary surgery.Due to the specificity and complexity of hepatobiliary surgery,traditional preoperative imaging techniques such as computed tomography and magnetic resonance imaging cannot meet the need for identification of fine anatomical regions.Imaging-based three-dimensional(3D)reconstruction,virtual simulation of surgery and 3D printing optimize the surgical plan through preoperative assessment,improving the controllability and safety of intraoperative operations,and in difficult-to-reach areas of the posterior and superior liver,assistive robots reproduce the surgeon’s natural movements with stable cameras,reducing natural vibrations.Electromagnetic navigation in abdominal surgery solves the problem of conventional surgery still relying on direct visual observation or preoperative image assessment.We summarize and compare these recent trends in digital medical solutions for the future development and refinement of digital medicine in hepatobiliary surgery.
基金financially supported by Tianjin Municipal Education Committee Scientific Research Project (No.2017KJ075)。
文摘Graphite anode materials are widely used in commercial lithium-ion batteries;however, the long electron/ion transportation path restricted its high energy storage. In this experiment, we designed a copper/graphite composite with a dual three-dimensional(3 D) continuous porous structure combining used nonsolvent-induced phase separation and heat treatment, in which a large amount of graphite is embedded in the 3 D porous copper/carbon architecture. In the novel structure, not only the electron and Li^(+) transmission performances are improved, but also the space of current collector is fully utilized. Meanwhile,carbonized polyacrylonitrile network stabilizes the interface between graphite and copper matrix. The obtained copper/graphite composite anode has an initial discharge capacity of 524.6 mAh·g^(-1), a holding capacity of350 mAh·g^(-1) and excellent cycle stability(299.3 mAh·g^(-1) after 180 cycles at 0.1 C rate), exhibiting good electrochemical performance. The experimental results show that the mass loading of the copper/graphite composite electrode material is about 4.39 mg·cm^(-2). We also envisage replacing graphite with other high-capacity active materials to fill the current collector, which can provide a reference for the future development of next-generation advanced electrodes.
基金Project supported by the National Natural Science Foundation of China
文摘On the basis of a three-dimensional weakly nonliear theory of Lagrangian residual current in the Baroclinic shallow seas, a diagnostic numerical calculation of wind-driven, thermohaline and tide-induced Lagrangian residual current in the Bohai Sea is made. The model involves the Richardson number in the eddy viscosity coefficient, wind, thcrmolialine and tidal effects in the focing terms. The runoff of the Huanghe River and a part of the Huanghai Warm Water coming from the Huanghai Sea through the Bohai Sea Strait is also considered. The velocity-splitting method is adopted. The wind-driven circu lation, thermohaline circulation and the tide-induced Lagrangian residual circulation are also obtained individually and analysed. The dynamics of the three main eddies in the Lagrangian mean circulation is discussed. Finally, the numerical result is partly verified with the observed data.
文摘The model equations with tbree-dimensional, time-dependent, nonlinear Navier-Stokes equations are transformed by sigma-transformation.On the basis of the process splitting technique, the fluid flow problems are divided into two parts:the vertically-intopated equations (external mode) and the vertical structure equations(internal mace). The first set of equations being the propagation of the tidal weves and the ADI numerical scheme has ben chosen to solve them. Conerning the vertical structure equations, they are solved by means of leapfrog stepping procedure.The main features of the tide and associated tidal current in the Bohai Sea are examined with this 3-D model.To have a good reproduction of vertical structure, the column is divided into 10 layers and the M2 tidal current is computed in detail. The simulation reveal the spetial structure and some important characteristics of the tidal current of the Bohai Sea. The application of the 3-D madel to forecasting of the tidal current in the Bobal Sea has been Performed as an illustration.
基金supported by the National Science Fund for Distinguished Young Scholars(42225107)the National Natural Science Foundation of China(42001326,42371414,42171409,and 42271419)+1 种基金the Natural Science Foundation of Guangdong Province of China(2022A1515012207)the Basic and Applied Basic Research Project of Guangzhou Science and Technology Planning(202201011539)。
文摘Three-dimensional(3D)urban structures play a critical role in informing climate mitigation strategies aimed at the built environment and facilitating sustainable urban development.Regrettably,there exists a significant gap in detailed and consistent data on 3D building space structures with global coverage due to the challenges inherent in the data collection and model calibration processes.In this study,we constructed a global urban structure(GUS-3D)dataset,including building volume,height,and footprint information,at a 500 m spatial resolution using extensive satellite observation products and numerous reference building samples.Our analysis indicated that the total volume of buildings worldwide in2015 exceeded 1×10^(12)m^(3).Over the 1985 to 2015 period,we observed a slight increase in the magnitude of 3D building volume growth(i.e.,it increased from 166.02 km3 during the 1985–2000 period to 175.08km3 during the 2000–2015 period),while the expansion magnitudes of the two-dimensional(2D)building footprint(22.51×10^(3) vs 13.29×10^(3)km^(2))and urban extent(157×10^(3) vs 133.8×10^(3)km^(2))notably decreased.This trend highlights the significant increase in intensive vertical utilization of urban land.Furthermore,we identified significant heterogeneity in building space provision and inequality across cities worldwide.This inequality is particularly pronounced in many populous Asian cities,which has been overlooked in previous studies on economic inequality.The GUS-3D dataset shows great potential to deepen our understanding of the urban environment and creates new horizons for numerous 3D urban studies.
文摘BACKGROUND Three-dimensional(3D)modelling technology translates the patient-specific anatomical information derived from two-dimensional radiological images into virtual or physical 3D models,which more closely resemble the complex environment encountered during surgery.It has been successfully applied to surgical planning and navigation,as well as surgical training and patient education in several surgical specialties,but its uptake lags behind in colorectal surgery.Rectal cancer surgery poses specific challenges due to the complex anatomy of the pelvis,which is difficult to comprehend and visualise.AIM To review the current and emerging applications of the 3D models,both virtual and physical,in rectal cancer surgery。METHODS Medline/PubMed,Embase and Scopus databases were searched using the keywords“rectal surgery”,“colorectal surgery”,“three-dimensional”,“3D”,“modelling”,“3D printing”,“surgical planning”,“surgical navigation”,“surgical education”,“patient education”to identify the eligible full-text studies published in English between 2001 and 2020.Reference list from each article was manually reviewed to identify additional relevant papers.The conference abstracts,animal and cadaveric studies and studies describing 3D pelvimetry or radiotherapy planning were excluded.Data were extracted from the retrieved manuscripts and summarised in a descriptive way.The manuscript was prepared and revised in accordance with PRISMA 2009 checklist.RESULTS Sixteen studies,including 9 feasibility studies,were included in the systematic review.The studies were classified into four categories:feasibility of the use of 3D modelling technology in rectal cancer surgery,preoperative planning and intraoperative navigation,surgical education and surgical device design.Thirteen studies used virtual models,one 3D printed model and 2 both types of models.The construction of virtual and physical models depicting the normal pelvic anatomy and rectal cancer,was shown to be feasible.Within the clinical context,3D models were used to identify vascular anomalies,for surgical planning and navigation in lateral pelvic wall lymph node dissection and in management of recurrent rectal cancer.Both physical and virtual 3D models were found to be valuable in surgical education,with a preference for 3D printed models.The main limitations of the current technology identified in the studies were related to the restrictions of the segmentation process and the lack of 3D printing materials that could mimic the soft and deformable tissues.CONCLUSION 3D modelling technology has potential to be utilised in multiple aspects of rectal cancer surgery,however,it is still at the experimental stage of application in this setting.
基金Supported by the 2022 Provincial Quality Engineering Project for Higher Education Institutions,No.2022sx031the 2023 Provincial Quality Engineering Project for Higher Education Institutions,No.2023jyxm1071.
文摘BACKGROUND Inguinal hernias are common after surgery.Tension-free repair is widely accepted as the main method for managing inguinal hernias.Adequate exposure,coverage,and repair of the myopectineal orifice(MPO)are necessary.However,due to differences in race and sex,people’s body shapes vary.According to European guidelines,the patch should measure 10 cm×15 cm.If any part of the MPO is dissected,injury to the nerves,vascular network,or organs may occur during surgery,thereby leading to inguinal discomfort,pain,and seroma formation after surgery.Therefore,accurate localization and measurement of the boundary of the MPO are crucial for selecting the optimal patch for inguinal hernia repair.AIM To compare the size of the MPO measured on three-dimensional multislice spiral computed tomography(CT)with that measured via laparoscopy and explore the relevant factors influencing the size of the MPO.METHODS Clinical data from 74 patients who underwent laparoscopic tension-free inguinal hernia repair at the General Surgery Department of the First Affiliated Hospital of Anhui University of Science and Technology between September 2022 and July 2024 were collected and analyzed retrospectively.Transabdominal preperitoneal was performed.Sixty-four males and 10 females,with an average age of 58.30±12.32 years,were included.The clinical data of the patients were collected.The boundary of the MPO was measured on three-dimensional CT images before surgery and then again during transabdominal preperitoneal.All the preoperative and intraoperative data were analyzed via paired t-tests.A t-test was used for comparisons of age,body mass index,and sex between the groups.In the comparative analysis,a P value less than 0.05 indicated a significant difference.RESULTS The boundaries of the MPO on 3-dimensional CT images measured 7.05±0.47 cm and 6.27±0.61 cm,and the area of the MPO was 19.54±3.33 cm^(2).The boundaries of the MPO during surgery were 7.18±0.51 cm and 6.17±0.40 cm.The errors were not statistically significant.However,the intraoperative BD(the width of the MPO,P=0.024,P<0.05)and preoperative AC(the length of the MPO,P=0.045,P<0.05)significantly differed according to sex.The AC and BD measurements before and during surgery were not significantly different according to age,body mass index,hernia side or hernia type(P>0.05).CONCLUSION The application of this technology can aid in determining the most appropriate dissection range and patch size.
文摘To address the problem of multi-missile cooperative interception against maneuvering targets at a prespecified impact time and desired Line-of-Sight(LOS)angles in ThreeDimensional(3D)space,this paper proposes a 3D leader-following cooperative interception guidance law.First,in the LOS direction of the leader,an impact time-controlled guidance law is derived based on the fixed-time stability theory,which enables the leader to complete the interception task at a prespecified impact time.Next,in the LOS direction of the followers,by introducing a time consensus tracking error function,a fixed-time consensus tracking guidance law is investigated to guarantee the consensus tracking convergence of the time-to-go.Then,in the direction normal to the LOS,by combining the designed global integral sliding mode surface and the second-order Sliding Mode Control(SMC)theory,an innovative 3D LOS-angle-constrained interception guidance law is developed,which eliminates the reaching phase in the traditional sliding mode guidance laws and effectively saves energy consumption.Moreover,it effectively suppresses the chattering phenomenon while avoiding the singularity issue,and compensates for unknown interference caused by target maneuvering online,making it convenient for practical engineering applications.Finally,theoretical proof analysis and multiple sets of numerical simulation results verify the effectiveness,superiority,and robustness of the investigated guidance law.
基金The Natural Science Foundation Study on Mechanics of Non-breaking wave-induced vertical mixing on Pollutant Dispersion of Huanghe River Estuary under contract No.51179178Project from Establishment of Fine Sediment Transport Modeling System for the Yellow Sea+1 种基金which is a sub-project of Development of Operational Oceanographic systemScience & Technology Development Project of Qingdao under contract No.09-1-3-18-jch
文摘A three-dimensional wave radiation stress is introduced into the hydrodynamic sediment coupled model COHERENS-SED, which has been developed through introducing wave-enhanced bottom shear stress, wave dependent surface drag coefficient, wave-induced surface mixing, SWAN, damping function of sediment on turbulence, sediment model and depth-dependent wave radiation stress to COHERENS. The COHERENS-SED is adopted to study the effects induced by wave-induced three-dimensional longshore current on suspended sediment spreading of the Huanghe River （Yellow River） mouth. Several different cases divided by setting different wave parameters of inputting boundary waves are carried out. The modeling results agree with measurement data. In terms of simulation results, it is easy to know that three-dimensional wave radiation stress plays an obvious role when inputting boundary wave height is stronger than 3 m. Moreover, wave direction also affects the sediment spreading rules of the mouth strongly too.
文摘The semidiagnostic and prognostic models are used to compute the current in the Taiwan Strait with wind and hydrographic data collected during August, 1984 and September 1 ~ 6, 1988. This calculation can be divided into two stages, i. e. (1 ) the adjustable stage; (2) prognostic calculation. The computed result shows tha the density and velocity fields etc. have been adjusted when t = 2. 5 d, namely the solution of semidignostic calculation is obtained,and the quasi-steady state solution have been reached after about 40 d. Comparing the results of diagncotic calculation with those of semidiagnortic and prognostic calculations indicates that they agree qualitatively. For example, they all have the following common features: (1 ) there is a persistent northward fiow with a volume transport of 0. 8 × 10 6 m3/s through the Taiwan Strait in summer; (2 ) the current near the western coas of Taiwan is stronger than that in other regiona; (3) the upweiling occurs near the Fujian coast and so on. However, there is a quantitative difference between them as follows. For example, the horizotal velocity near the westem cot of Taiwan and the upwelling speed near the Fujian aret both are underestimaed in the diagnestic calculation, because the data used in which is smoothed, and they both are intensified in the solutions of semidiagnostic and prognostic calculations. For example, the maximum velocity near the western coast of Taiwan at t = 0 d (diagnostic), 2. 5 d (semidiagnostic) and 300 d (prognostic) is 59.1, 62. 1 and 62. 0 cm/s, respectively. From the above comparison we see it is quite necessary that a semidiagnostic model be used to compute the currents when the data have been smoothed.
基金financially supported by the the National Natural Science Foundation of China(Grant No.51709054)the Public Science and Technology Research Funds Projects of Ocean(Grant Nos.201405025 and 201505019)
文摘By coupling the three-dimensional hydrodynamic model with the wave model, numerical simulations of the three- dimensional wave-induced current are carried out in this study. The wave model is based on the numerical solution of the modified wave action equation and eikonal equation, which can describe the wave refraction and diffraction. The hydrodynamic model is driven by the wave-induced radiation stresses and affected by the wave turbulence. The numerical implementation of the module has used the finite-volume schemes on unstructured grid, which provides great flexibility for modeling the waves and currents in the complex actual nearshore, and ensures the conservation of energy propagation. The applicability of the proposed model is evaluated in calculating the cases of wave set-up, longshore currents, undertow on a sloping beach, rip currents and meandering longshore currents on a tri-cuspate beach. The results indicate that it is necessary to introduce the depth-dependent radiation stresses into the numerical simulation of wave-induced currents, and comparisons show that the present model makes better prediction on the wave procedure as well as both horizontal and vertical structures in the wave-induced current field.
基金National Natural Science Foundation of China under contract No. 40176007National Key Foundation Research Program of China under contract No. G1999043802
文摘Based on the wind and hydrographic data obtained by R/V Xiangyanghong 14 during June of 1999, the currents in the Huanghai Sea and East China Sea are computed by the three dimensional non-linear diagnostic, semidiagnostic models and prognostic in the a coordinate. The computed results show that the density and velocity fields and so on have been adjusted when time is about 3 days, namely the solution of semidiagnostic calculation is obtained. In the northwest part of the computed region, the Huanghai coastal current flows southeastward, and then it flows out the computed region south of Cheju Island. In the west side of the southern part of the computed region, there is other current, which is mainly inshore branch of Taiwan Warm Current, and it flows cyclonically and turns to the northeast. In the region north of the above two currents, there is a cyclonic eddy southwest of Cheju Island, and it has characteristics of high density and low temperature. There is an offshore branch of Taiwan Warm Current in the west side of the Kuroshio, and it makes a cyclonic meander, then flows northeastward. The Kuroshio in the East China Sea is stronger, and flows northeastward. Its maximum horizontal velocity is 108.5 cm/s at the sea surface, which is located at the northern boundary, and it is 106.1 cm/s at 30 m level, 102.2 cm/s at 75 m level and 85.1 cm/s at 200 m level, respectively, which are all located at the southern boundary. Comparing the results of diagnostic calculation with those of semidiagnostic and prognostic calculations indicates that the horizontal velocity field agrees qualitatively, and there is a little difference between them in quantity. The comparison between the computed velocities and the observed velocities at the mooring station show that they agree each other.
基金supported by grants from the Human Resources Development program (Grant No.20204010600250)the Training Program of CCUS for the Green Growth (Grant No.20214000000500)by the Korea Institute of Energy Technology Evaluation and Planning (KETEP)funded by the Ministry of Trade,Industry,and Energy of the Korean Government (MOTIE).
文摘It is of great importance to obtain precise trace data,as traces are frequently the sole visible and measurable parameter in most outcrops.The manual recognition and detection of traces on high-resolution three-dimensional(3D)models are relatively straightforward but time-consuming.One potential solution to enhance this process is to use machine learning algorithms to detect the 3D traces.In this study,a unique pixel-wise texture mapper algorithm generates a dense point cloud representation of an outcrop with the precise resolution of the original textured 3D model.A virtual digital image rendering was then employed to capture virtual images of selected regions.This technique helps to overcome limitations caused by the surface morphology of the rock mass,such as restricted access,lighting conditions,and shading effects.After AI-powered trace detection on two-dimensional(2D)images,a 3D data structuring technique was applied to the selected trace pixels.In the 3D data structuring,the trace data were structured through 2D thinning,3D reprojection,clustering,segmentation,and segment linking.Finally,the linked segments were exported as 3D polylines,with each polyline in the output corresponding to a trace.The efficacy of the proposed method was assessed using a 3D model of a real-world case study,which was used to compare the results of artificial intelligence(AI)-aided and human intelligence trace detection.Rosette diagrams,which visualize the distribution of trace orientations,confirmed the high similarity between the automatically and manually generated trace maps.In conclusion,the proposed semi-automatic method was easy to use,fast,and accurate in detecting the dominant jointing system of the rock mass.
基金Project supported by the National Natural Science Foundation of China(Nos.12372071 and 12372070)the Aeronautical Science Fund of China(No.2022Z055052001)the Foundation of China Scholarship Council(No.202306830079)。
文摘Currently,there are a limited number of dynamic models available for braided composite plates with large overall motions,despite the incorporation of three-dimensional(3D)braided composites into rotating blade components.In this paper,a dynamic model of 3D 4-directional braided composite thin plates considering braiding directions is established.Based on Kirchhoff's plate assumptions,the displacement variables of the plate are expressed.By incorporating the braiding directions into the constitutive equation of the braided composites,the dynamic model of the plate considering braiding directions is obtained.The effects of the speeds,braiding directions,and braided angles on the responses of the plate with fixed-axis rotation and translational motion,respectively,are investigated.This paper presents a dynamic theory for calculating the deformation of 3D braided composite structures undergoing both translational and rotational motions.It also provides a simulation method for investigating the dynamic behavior of non-isotropic material plates in various applications.
文摘Liposarcoma is one of the most common soft tissue sarcomas,however,its occurrence rate is still rare compared to other cancers.Due to its rarity,in vitro experiments are an essential approach to elucidate liposarcoma pathobiology.Conventional cell culture-based research(2D cell culture)is still playing a pivotal role,while several shortcomings have been recently under discussion.In vivo,mouse models are usually adopted for pre-clinical analyses with expectations to overcome the issues of 2D cell culture.However,they do not fully recapitulate human dedifferentiated liposarcoma(DDLPS)characteristics.Therefore,three-dimensional(3D)culture systems have been the recent research focus in the cell biology field with the expectation to overcome at the same time the disadvantages of 2D cell culture and in vivo animal models and fill in the gap between them.Given the liposarcoma rarity,we believe that 3D cell culture techniques,including 3D cell cultures/co-cultures,and Patient-Derived tumor Organoids(PDOs),represent a promising approach to facilitate liposarcoma investigation and elucidate its molecular mechanisms and effective therapy development.In this review,we first provide a general overview of 3D cell cultures compared to 2D cell cultures.We then focus on one of the recent 3D cell culture applications,Patient-Derived Organoids(PDOs),summarizing and discussing several PDO methodologies.Finally,we discuss the current and future applications of PDOs to sarcoma,particularly in the field of liposarcoma.
基金supported by the National Science Foundation of China(Nos.81373970,81773902,81973484,and 32171402)the National College Students Innovation and Entrepreneurship Training Program(No.201810315019)+4 种基金the Postgraduate Research and Practice Innovation Program of Jiangsu Province(Nos.SJCX21_0712 and KYCX23_2052)the Scientific Research Project of Jiangsu Provincial Association of Traditional Chinese Medicine(No.XYLD2024013)the Youth Scientific Research Project of Jiangyin Municipal Health Commission(No.Q202402)the Natural Science Foundation Project of Nanjing University of Chinese Medicine(No.XZR2024173)the Jiangyin Science and Technology Innovation Special Fund Project(No.JY0603A011014230032PB),China.
文摘Bone repair remains an important target in tissue engineering,making the development of bioactive scaffolds for effective bone defect repair a critical objective.In this study,β-tricalcium phosphate(β-TCP)scaffolds incorporated with processed pyritum decoction(PPD)were fabricated using three-dimensional(3D)printing-assisted freeze-casting.The produced composite scaffolds were evaluated for their mechanical strength,physicochemical properties,biocompatibility,in vitro proangiogenic activity,and in vivo efficacy in repairing rabbit femoral defects.They not only demonstrated excellent physicochemical properties,enhanced mechanical strength,and good biosafety but also significantly promoted the proliferation,migration,and aggregation of pro-angiogenic human umbilical vein endothelial cells(HUVECs).In vivo studies revealed that all scaffold groups facilitated osteogenesis at the bone defect site,with theβ-TCP scaffolds loaded with PPD markedly enhancing the expression of neurogenic locus Notch homolog protein 1(Notch1),vascular endothelial growth factor(VEGF),bone morphogenetic protein-2(BMP-2),and osteopontin(OPN).Overall,the scaffolds developed in this study exhibited strong angiogenic and osteogenic capabilities both in vitro and in vivo.The incorporation of PPD notably promoted the angiogenic-osteogenic coupling,thereby accelerating bone repair,which suggests that PPD is a promising material for bone repair and that the PPD/β-TCP scaffolds hold great potential as a bone graft alternative.
