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Global Mapping of Three-Dimensional Urban Structures Reveals Escalating Utilization in the Vertical Dimension and Pronounced Building Space Inequality
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作者 Xiaoping Liu Xinxin Wu +6 位作者 Xuecao Li Xiaocong Xu Weilin Liao Limin Jiao Zhenzhong Zeng Guangzhao Chen Xia Li 《Engineering》 2025年第4期86-99,共14页
Three-dimensional(3D)urban structures play a critical role in informing climate mitigation strategies aimed at the built environment and facilitating sustainable urban development.Regrettably,there exists a significan... Three-dimensional(3D)urban structures play a critical role in informing climate mitigation strategies aimed at the built environment and facilitating sustainable urban development.Regrettably,there exists a significant gap in detailed and consistent data on 3D building space structures with global coverage due to the challenges inherent in the data collection and model calibration processes.In this study,we constructed a global urban structure(GUS-3D)dataset,including building volume,height,and footprint information,at a 500 m spatial resolution using extensive satellite observation products and numerous reference building samples.Our analysis indicated that the total volume of buildings worldwide in2015 exceeded 1×10^(12)m^(3).Over the 1985 to 2015 period,we observed a slight increase in the magnitude of 3D building volume growth(i.e.,it increased from 166.02 km3 during the 1985–2000 period to 175.08km3 during the 2000–2015 period),while the expansion magnitudes of the two-dimensional(2D)building footprint(22.51×10^(3) vs 13.29×10^(3)km^(2))and urban extent(157×10^(3) vs 133.8×10^(3)km^(2))notably decreased.This trend highlights the significant increase in intensive vertical utilization of urban land.Furthermore,we identified significant heterogeneity in building space provision and inequality across cities worldwide.This inequality is particularly pronounced in many populous Asian cities,which has been overlooked in previous studies on economic inequality.The GUS-3D dataset shows great potential to deepen our understanding of the urban environment and creates new horizons for numerous 3D urban studies. 展开更多
关键词 three-dimensionAL Global mapping Building volume Building height Building space inequality
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Three-dimension collision-free trajectory planning of UAVs based on ADS-B information in low-altitude urban airspace 被引量:2
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作者 Chao DONG Yifan ZHANG +3 位作者 Ziye JIA Yiyang LIAO Lei ZHANG Qihui WU 《Chinese Journal of Aeronautics》 2025年第2期274-285,共12页
The environment of low-altitude urban airspace is complex and variable due to numerous obstacles,non-cooperative aircraft,and birds.Unmanned Aerial Vehicles(UAVs)leveraging environmental information to achieve three-d... The environment of low-altitude urban airspace is complex and variable due to numerous obstacles,non-cooperative aircraft,and birds.Unmanned Aerial Vehicles(UAVs)leveraging environmental information to achieve three-dimension collision-free trajectory planning is the prerequisite to ensure airspace security.However,the timely information of surrounding situation is difficult to acquire by UAVs,which further brings security risks.As a mature technology leveraged in traditional civil aviation,the Automatic Dependent Surveillance-Broadcast(ADS-B)realizes continuous surveillance of the information of aircraft.Consequently,we leverage ADS-B for surveillance and information broadcasting,and divide the aerial airspace into multiple sub-airspaces to improve flight safety in UAV trajectory planning.In detail,we propose the secure Sub-airSpaces Planning(SSP)algorithm and Particle Swarm Optimization Rapidly-exploring Random Trees(PSO-RRT)algorithm for the UAV trajectory planning in law-altitude airspace.The performance of the proposed algorithm is verified by simulations and the results show that SSP reduces both the maximum number of UAVs in the sub-airspace and the length of the trajectory,and PSO-RRT reduces the cost of UAV trajectory in the sub-airspace. 展开更多
关键词 three-dimension trajectory planning of UAV Collision avoidance Sliding window ADS-B Low-altitude urban airspace
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Navigating anatomical complexity in laparoscopic sigmoid cancer surgery:A three-dimension reconstruction protocol for intraoperative safety and efficiency
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作者 Zong-Xian Zhao Run-Dong Yao +3 位作者 Zong-Ju Hu Chao-Qian Chen Shu Zhu Yuan Yao 《World Journal of Gastrointestinal Surgery》 2025年第8期350-361,共12页
BACKGROUND Sigmoid colon cancer faces challenges due to anatomical diversity,including variable inferior mesenteric artery(IMA)branching and tumor localization complexities,which increase intraoperative risks.AIM To c... BACKGROUND Sigmoid colon cancer faces challenges due to anatomical diversity,including variable inferior mesenteric artery(IMA)branching and tumor localization complexities,which increase intraoperative risks.AIM To comprehensively evaluate the impact of three-dimensional(3D)visualization technology on enhancing surgical precision and safety,as well as optimizing perioperative outcomes in laparoscopic sigmoid cancer resection.METHODS A prospective cohort of 106 patients(January 2023 to December 2024)undergoing laparoscopic sigmoid cancer resection was divided into the 3D(n=55)group and the control(n=51)group.The 3D group underwent preoperative enhanced computed tomography reconstruction(3D Slicer 5.2.2&Mimics 19.0).3D reconstruction visualization navigation intraoperatively guided the following key steps:Tumor location,Toldt’s space dissection,IMA ligation level selection,regional lymph node dissection,and marginal artery preservation.Outcomes included operative parameters,lymph node yield,and recovery metrics.RESULTS The 3D group demonstrated a significantly shorter operative time(172.91±20.69 minutes vs 190.29±32.29 minutes;P=0.002),reduced blood loss(31.5±11.8 mL vs 44.1±23.4 mL,P=0.001),earlier postoperative flatus(2.23±0.54 days vs 2.53±0.61 days;P=0.013),shorter hospital length of stay(13.47±1.74 days vs 16.20±7.71 days;P=0.013),shorter postoperative length of stay(8.6±2.6 days vs 10.5±4.9 days;P=0.014),and earlier postoperative exhaust time(2.23±0.54 days vs 2.53±0.61 days;P=0.013).Furthermore,the 3D group exhibited a higher mean number of lymph nodes harvested(16.91±5.74 vs 14.45±5.66;P=0.030).CONCLUSION The 3D visualization technology effectively addresses sigmoid colon anatomical complexity through surgical navigation,improving procedural safety and efficiency. 展开更多
关键词 three-dimension reconstruction Sigmoid colon cancer Visualization Inferior mesenteric artery Anatomical complexity Intraoperative safety
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Three-Dimensional Prospectivity Modeling of Jinshan Ag-Au Deposit,Southern China by Weights-of-Evidence
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作者 Fan Xiao Qiuming Cheng +1 位作者 Weisheng Hou Frederik P.Agterberg 《Journal of Earth Science》 2025年第5期2038-2057,共20页
To comprehensively utilize the valuable geological map,exploration profile,borehole,and geochemical logging data and the knowledge on the formation of the Jinshan Ag-Au deposit for forecasting the exploration targets ... To comprehensively utilize the valuable geological map,exploration profile,borehole,and geochemical logging data and the knowledge on the formation of the Jinshan Ag-Au deposit for forecasting the exploration targets of concealed ore bodies,three-dimensional Mineral Prospectivity Modeling(MPM)of the deposit has been conducted using the weights-of-evidence(WofE)method.Conditional independence between evidence layers was tested,and the outline results using the prediction-volume(P-V)and Student's t-statistic methods for delineating favorable mineralization areas from continuous posterior probability map were critically compared.Four exploration targets delineated ultimately by the Student's t-statistic method for the discovery of minable ore bodies in each of the target areas were discussed in detail.The main conclusions include:(1)three-dimensional modeling of a deposit using multi-source reconnaissance data is useful for MPM in interpreting their relationships with known ore bodies;(2)WofE modeling can be used as a straightforward tool for integrating deposit model and reconnaissance data in MPM;(3)the Student's t-statistic method is more applicable in binarizing the continuous prospectivity map for exploration targeting than the PV approach;and(4)two target areas within high potential to find undiscovered ore bodies were diagnosed to guide future near-mine exploration activities of the Jinshan deposit. 展开更多
关键词 three-dimensional modeling mineral prospectivity mapping exploration targeting WEIGHTS-OF-EVIDENCE C-V fractal model Jinshan Ag-Au deposit mineral deposits economic geology
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Artificial intelligence-aided semi-automatic joint trace detection from textured three-dimensional models of rock mass
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作者 Seyedahmad Mehrishal Jineon Kim +1 位作者 Yulong Shao Jae Joon Song 《Journal of Rock Mechanics and Geotechnical Engineering》 2025年第4期1973-1985,共13页
It is of great importance to obtain precise trace data,as traces are frequently the sole visible and measurable parameter in most outcrops.The manual recognition and detection of traces on high-resolution three-dimens... It is of great importance to obtain precise trace data,as traces are frequently the sole visible and measurable parameter in most outcrops.The manual recognition and detection of traces on high-resolution three-dimensional(3D)models are relatively straightforward but time-consuming.One potential solution to enhance this process is to use machine learning algorithms to detect the 3D traces.In this study,a unique pixel-wise texture mapper algorithm generates a dense point cloud representation of an outcrop with the precise resolution of the original textured 3D model.A virtual digital image rendering was then employed to capture virtual images of selected regions.This technique helps to overcome limitations caused by the surface morphology of the rock mass,such as restricted access,lighting conditions,and shading effects.After AI-powered trace detection on two-dimensional(2D)images,a 3D data structuring technique was applied to the selected trace pixels.In the 3D data structuring,the trace data were structured through 2D thinning,3D reprojection,clustering,segmentation,and segment linking.Finally,the linked segments were exported as 3D polylines,with each polyline in the output corresponding to a trace.The efficacy of the proposed method was assessed using a 3D model of a real-world case study,which was used to compare the results of artificial intelligence(AI)-aided and human intelligence trace detection.Rosette diagrams,which visualize the distribution of trace orientations,confirmed the high similarity between the automatically and manually generated trace maps.In conclusion,the proposed semi-automatic method was easy to use,fast,and accurate in detecting the dominant jointing system of the rock mass. 展开更多
关键词 Automatic trace detection Digital joint mapping Rock discontinuities characterization three-dimensional(3D)trace network
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A physics-informed machine learning solution for landslide susceptibility mapping based on three-dimensional slope stability evaluation
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作者 WANG Yun-hao WANG Lu-qi +4 位作者 ZHANG Wen-gang LIU Song-lin SUN Wei-xin HONG Li ZHU Zheng-wei 《Journal of Central South University》 CSCD 2024年第11期3838-3853,共16页
Landslide susceptibility mapping is a crucial tool for disaster prevention and management.The performance of conventional data-driven model is greatly influenced by the quality of the samples data.The random selection... Landslide susceptibility mapping is a crucial tool for disaster prevention and management.The performance of conventional data-driven model is greatly influenced by the quality of the samples data.The random selection of negative samples results in the lack of interpretability throughout the assessment process.To address this limitation and construct a high-quality negative samples database,this study introduces a physics-informed machine learning approach,combining the random forest model with Scoops 3D,to optimize the negative samples selection strategy and assess the landslide susceptibility of the study area.The Scoops 3D is employed to determine the factor of safety value leveraging Bishop’s simplified method.Instead of conventional random selection,negative samples are extracted from the areas with a high factor of safety value.Subsequently,the results of conventional random forest model and physics-informed data-driven model are analyzed and discussed,focusing on model performance and prediction uncertainty.In comparison to conventional methods,the physics-informed model,set with a safety area threshold of 3,demonstrates a noteworthy improvement in the mean AUC value by 36.7%,coupled with a reduced prediction uncertainty.It is evident that the determination of the safety area threshold exerts an impact on both prediction uncertainty and model performance. 展开更多
关键词 machine learning physics-informed model negative samples selection INTERPRETABILITY landslide susceptibility mapping
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Real-time volume rendering for three-dimensional fetal ultrasound using volumetric photon mapping
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作者 Jing Zou Jing Qin 《Visual Computing for Industry,Biomedicine,and Art》 2024年第1期83-97,共15页
Three-dimensional(3D)fetal ultrasound has been widely used in prenatal examinations.Realistic and real-time volumetric ultrasound volume rendering can enhance the effectiveness of diagnoses and assist obstetricians an... Three-dimensional(3D)fetal ultrasound has been widely used in prenatal examinations.Realistic and real-time volumetric ultrasound volume rendering can enhance the effectiveness of diagnoses and assist obstetricians and pregnant mothers in communicating.However,this remains a challenging task because(1)there is a large amount of speckle noise in ultrasound images and(2)ultrasound images usually have low contrasts,making it difficult to distinguish different tissues and organs.However,traditional local-illumination-based methods do not achieve satisfactory results.This real-time requirement makes the task increasingly challenging.This study presents a novel real-time volume-rendering method equipped with a global illumination model for 3D fetal ultrasound visualization.This method can render direct illumination and indirect illumination separately by calculating single scattering and multiple scattering radiances,respectively.The indirect illumination effect was simulated using volumetric photon mapping.Calculating each photon’s brightness is proposed using a novel screen-space destiny estimation to avoid complicated storage structures and accelerate computation.This study proposes a high dynamic range approach to address the issue of fetal skin with a dynamic range exceeding that of the display device.Experiments show that our technology,compared to conventional methodologies,can generate realistic rendering results with far more depth information. 展开更多
关键词 three-dimensional fetal ultrasound Volume rendering Photon mapping Global illumination
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3.0T磁共振T2 mapping序列联合血清新饱食分子蛋白1水平检测在老年膝关节早期骨关节炎诊断中的应用价值
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作者 唐利 巩玉荣 +2 位作者 曾立叶 高艳芳 邓成哲 《实用医学杂志》 北大核心 2025年第8期1238-1242,共5页
目的探讨3.0 T磁共振(MRI)T2 mapping序列联合血清新饱食分子蛋白1(nesfatin-1)水平对老年膝关节早期骨关节炎(OA)的诊断价值。方法选取2023年5月至2024年5月医院收治的膝关节OA的97例老年患者(OA组)和52例同期老年体检者(对照组),根据... 目的探讨3.0 T磁共振(MRI)T2 mapping序列联合血清新饱食分子蛋白1(nesfatin-1)水平对老年膝关节早期骨关节炎(OA)的诊断价值。方法选取2023年5月至2024年5月医院收治的膝关节OA的97例老年患者(OA组)和52例同期老年体检者(对照组),根据X线结果将膝关节OA组分为早期组和非早期组,均接受3.0T MRI T2 mapping序列扫描检测膝关节软骨区域T2值,检测血清nesfatin-1水平,比较上述指标差异并采用ROC曲线分析其对老年膝关节早期OA的诊断价值。结果97例老年膝关节OA患者中,早期组35例,非早期组62例,OA组患者膝关节5个软骨区域的T2值及nesfatin-1血清均高于对照组(P<0.05),早期组均低于非早期组(P<0.05);膝关节软骨区域T2值和血清nesfatin-1水平单独诊断早期OA的AUC在0.774~0.871范围,联合诊断的AUC为0.939。结论3.0 T磁共振T2 mapping序列联合血清nesfatin-1水平检测对老年膝关节早期OA具有较高的诊断价值。 展开更多
关键词 膝关节早期骨关节炎 老年 磁共振 T2 mapping序列 新饱食分子蛋白1
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MR T_(1)-mapping结合动脉自旋标记技术在评估儿童慢性肾脏病肾纤维化和灌注中的应用 被引量:1
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作者 赵佳佳 胡俊 +2 位作者 尹传高 顾东浩 朱晓冬 《临床放射学杂志》 北大核心 2025年第3期513-517,共5页
目的初步研究MR纵向弛豫时间定量成像(T 1-mapping)和动脉自旋标记技术(ASL)在诊断儿童慢性肾脏病(CKD)中的应用价值。方法纳入CKD患儿32例(包括肾脏未萎缩19例,萎缩13例),同期招募健康志愿者30名作对照组,行T 1-mapping和ASL序列扫描,... 目的初步研究MR纵向弛豫时间定量成像(T 1-mapping)和动脉自旋标记技术(ASL)在诊断儿童慢性肾脏病(CKD)中的应用价值。方法纳入CKD患儿32例(包括肾脏未萎缩19例,萎缩13例),同期招募健康志愿者30名作对照组,行T 1-mapping和ASL序列扫描,分别获得肾皮质T 1值和肾皮质血流量(RBF)。比较对照组和CKD组患儿肾皮质T 1值和RBF值的差异,并分析CKD组肾皮质T 1值和RBF值与估算肾小球滤过率(eGFR)、血肌酐(Scr)、血胱抑素C(Cys-C)的相关性。结果CKD组较对照组肾皮质T 1值明显升高、RBF值明显减低(P<0.05);CKD组中,肾脏未萎缩患儿肾皮质T 1值与eGRF、Scr、Cys-C呈高、中度相关(r=-0.832、0.777、0.698,P<0.05),肾脏萎缩患儿肾皮质T 1值与eGRF、Scr呈高度相关(r=0.726、-0.743,P<0.05);CKD组肾皮质RBF与eGRF、Cys-C、Scr呈中度相关(r=0.699、-0.619、-0.554,P<0.05)。结论MR T_(1)-mapping和ASL定量反映肾脏微观病理变化及血流灌注情况,具有预测肾脏慢性病理损伤的潜在价值,有望在肾脏发生不可逆损伤前早期、无创地提供肾脏功能信息。 展开更多
关键词 慢性肾脏病 儿童 T_(1)-mapping 动脉自旋标记 磁共振功能成像
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基于ArcMap的森林资源调查地形图接图网制作
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作者 胡卫东 龙艳 《计算机应用文摘》 2025年第9期176-179,182,共5页
文章以广西森林资源调查1∶1万地形图接图网的制作过程为例,利用ArcMap 10.8的渔网工具、创建自定义地理(坐标)变换工具和投影工具,制作了基于CGCS2000坐标系的1∶1万地形图接图网,以及1954北京坐标系转CGCS2000坐标系和1980西安坐标系... 文章以广西森林资源调查1∶1万地形图接图网的制作过程为例,利用ArcMap 10.8的渔网工具、创建自定义地理(坐标)变换工具和投影工具,制作了基于CGCS2000坐标系的1∶1万地形图接图网,以及1954北京坐标系转CGCS2000坐标系和1980西安坐标系转CGCS2000坐标系的1∶1万地形图接图网,旨在通过接图网,在CGCS2000坐标系下,快速、准确、高效地完成不同坐标系下的1∶1万地形图栅格配准。 展开更多
关键词 森林资源调查 ARCmap 地形图 接图网 配准
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Three-dimensional computed tomography mapping of posterior malleolar fractures 被引量:2
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作者 Qi-Hang Su Juan Liu +5 位作者 Yan Zhang Jun Tan Mei-Jun Yan Kai Zhu Jin Zhang Cong Li 《World Journal of Clinical Cases》 SCIE 2020年第1期29-37,共9页
BACKGROUND Posterior malleolar fractures have been reported to occur in<40%of ankle fractures.AIM To reveal the recurrent patterns and characteristics of posterior malleolar fractures by creating fracture maps of t... BACKGROUND Posterior malleolar fractures have been reported to occur in<40%of ankle fractures.AIM To reveal the recurrent patterns and characteristics of posterior malleolar fractures by creating fracture maps of the posterior malleolar fractures through the use of computed tomography mapping.METHODS A consecutive series of posterior malleolar fractures was used to create threedimensional reconstruction images,which were oriented and superimposed to fit an ankle model template by both aligning specific biolandmarks and reducing reconstructed fracture fragments.Fracture lines were found and traced in order to generate an ankle fracture map.RESULTS This study involved 112 patients with a mean age of 49,comprising 32 pronationexternal rotation grade IV fractures and 80 supination-external rotation grade IV fractures according to the Lauge-Hansen classification system.Three-dimensional maps showed that the posterior ankle fracture fragments in the supinationexternal rotation grade IV group were relatively smaller than those in the pronation-external rotation grade IV group after posterior malleolus fracture.In addition,the distribution analyses on posterior malleolus fracture lines indicated that the supination-external rotation grade IV group tended to have higher linear density but more concentrated and orderly distribution fractures compared to the pronation-external rotation grade IV group.CONCLUSION Fracture maps revealed the fracture characteristics and recurrent patterns of posterior malleolar fractures,which might help to improve the understanding of ankle fracture as well as increase opportunities for follow-up research and aid clinical decision-making. 展开更多
关键词 three-dimensional imaging mapS Ankle fractures Computed tomography
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T2 mapping与3D-WATSc序列定量测量膝关节软骨体积的一致性 被引量:1
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作者 吴明杰 许亚飞 +2 位作者 傅孙亚 张玉琴 徐凌斌 《中国医学影像学杂志》 北大核心 2025年第3期320-325,共6页
目的探索T2 mapping与三维选择性水激发(3D-WATSc)序列测量膝关节软骨体积的一致性。资料与方法2022年5—12月宁波大学附属李惠利医院招募30名健康志愿者,行左膝关节3D-WATSc、T2mapping序列扫描。随机抽取20名受试者的T2 mapping序列,... 目的探索T2 mapping与三维选择性水激发(3D-WATSc)序列测量膝关节软骨体积的一致性。资料与方法2022年5—12月宁波大学附属李惠利医院招募30名健康志愿者,行左膝关节3D-WATSc、T2mapping序列扫描。随机抽取20名受试者的T2 mapping序列,分析8组不同TE(13、26、39、52、65、78、91、104 ms)的原始图像,由2名观察者对原始图像质量进行评分,使用Kappa检验观察评分一致性,Welch方差分析和Games-Howell text事后检验得出最佳图像质量的TE。基于T2 mapping和3D-WATSc序列对30名受试者股骨髁、胫骨平台和髌骨后缘软骨体积进行半自动分割测量,使用组内相关系数(ICC)、BlandAltman检验T2 mapping序列下2名观察者间及两种序列测得软骨体积的一致性。结果2名观察者评分除TE 104 ms一致性较差(Kappa=0.318)、TE 78 ms差异无统计学意义(P=0.052)外,其余一致性均为中等(Kappa>0.40,P<0.05),进一步分析TE 26 ms软骨显示质量评分最佳(均值2.90)。将TE 26 ms与其余7组评分进行Games-Howell text两两比较,除与TE 13 ms(均值2.55)、TE 39 ms(均值2.62)两组评分差异无统计学意义(P>0.05)外,TE 26 ms与其余TE组评分差异均有统计学意义(P均<0.05)。在T2 mapping(TR 2000 ms、TE 26 ms)序列上,2名观察者分割得到各部分软骨体积的ICC均>0.6,P<0.05,以股骨髁软骨体积的一致性最好(ICC=0.877,P<0.01)。两种序列测得的各部分软骨体积一致性均较好(ICC均>0.75,P均<0.01),以胫骨平台软骨体积的一致性最好(ICC=0.857,P<0.01)。结论T2 mapping原始图像(TR 2000 ms、TE 26 ms)可清晰显示膝关节软骨形态,T2 mapping与3D-WATSc序列定量膝关节软骨体积的一致性较好,T2 mapping单一序列可为膝关节软骨形态及功能的一站式定量分析提供可信的扫描方案。 展开更多
关键词 磁共振成像 T2 mapping 三维选择性水激发序列 膝关节 软骨 体积
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Asymmetric image encryption algorithm based on a new three-dimensional improved logistic chaotic map 被引量:1
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作者 叶国栋 吴惠山 +1 位作者 黄小玲 Syh-Yuan Tan 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第3期153-163,共11页
Based on some analyses of existing chaotic image encryption frameworks and a new designed three-dimensional improved logistic chaotic map(3D-ILM),an asymmetric image encryption algorithm using public-key Rivest–Shami... Based on some analyses of existing chaotic image encryption frameworks and a new designed three-dimensional improved logistic chaotic map(3D-ILM),an asymmetric image encryption algorithm using public-key Rivest–Shamir–Adleman(RSA)is presented in this paper.In the first stage,a new 3D-ILM is proposed to enhance the chaotic behavior considering analysis of time sequence,Lyapunov exponent,and Shannon entropy.In the second stage,combined with the public key RSA algorithm,a new key acquisition mathematical model(MKA)is constructed to obtain the initial keys for the 3D-ILM.Consequently,the key stream can be produced depending on the plain image for a higher security.Moreover,a novel process model(NPM)for the input of the 3D-ILM is built,which is built to improve the distribution uniformity of the chaotic sequence.In the third stage,to encrypt the plain image,a pre-process by exclusive OR(XOR)operation with a random matrix is applied.Then,the pre-processed image is performed by a permutation for rows,a downward modulo function for adjacent pixels,a permutation for columns,a forward direction XOR addition-modulo diffusion,and a backward direction XOR addition-modulo diffusion to achieve the final cipher image.Moreover,experiments show that the the proposed algorithm has a better performance.Especially,the number of pixels change rate(NPCR)is close to ideal case 99.6094%,with the unified average changing intensity(UACI)close to 33.4634%,and the information entropy(IE)close to 8. 展开更多
关键词 three-dimensional improved logistic chaotic map(3D-ILM) Rivest–Shamir–Adleman(RSA)algorithm image encryption CONFUSION ENTROPY
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基于副结核分枝杆菌重组MAP0827c蛋白的间接ELISA抗体检测方法的建立与初步应用 被引量:1
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作者 俞杰 许淑芸 +3 位作者 魏京京 魏增科 赵昌乐 周霞 《中国预防兽医学报》 北大核心 2025年第3期271-277,共7页
为建立快速准确检测副结核分枝杆菌(MAP)抗体的间接ELISA方法,本研究通过SignalP-5.0等相关网站分析MAP0827c蛋白的生物信息学特点,设计扩增MAP MAP0827c基因的引物,将扩增的MAP0827c基因克隆于pET-32a(+)中构建重组质粒pET32a-MAP0827c... 为建立快速准确检测副结核分枝杆菌(MAP)抗体的间接ELISA方法,本研究通过SignalP-5.0等相关网站分析MAP0827c蛋白的生物信息学特点,设计扩增MAP MAP0827c基因的引物,将扩增的MAP0827c基因克隆于pET-32a(+)中构建重组质粒pET32a-MAP0827c,并经酶切和测序鉴定正确后利用原核表达系统表达重组MAP0827c蛋白,以该蛋白作为包被抗原,采用棋盘滴定法筛选间接ELISA反应条件,确定临界值,以异源阳性血清验证该方法的特异性;通过稀释阳性血清评估所建方法的灵敏性;以5份牛MAP阳性血清进行该方法的批内和批间试验评估该方法的重复性。结果显示,MAP0827c蛋白由374个氨基酸构成,含15个抗原决定簇,不存在跨膜区和信号肽,二级结构以α螺旋为主。经原核系统表达了分子量为42 ku的目的蛋白。建立的ELISA方法蛋白最佳包被量为0.05μg/mL,一抗血清最佳稀释倍数为1:100,二抗兔抗牛IgG-HRP的最佳稀释倍数为1:5000,封闭液选择1%明胶封闭1 h,一抗血清作用时为1 h,酶标二抗孵育时间为1 h,显色时间为25 min;特异性试验结果显示,建立的间接ELISA方法仅对MAP阳性血清检测为阳性,而不与布鲁氏菌、结核分枝杆菌及金黄色葡萄球菌的牛阳性血清发生反应,特异性强;敏感性试验结果显示,可检测的阳性血清最大稀释度为1:800,敏感性高;批内和批间重复试验结果显示,变异系数均小于10%,重复性较好。采用建立的ELISA方法和商品化ELISA试剂盒对70份临床牛血清样品进行检测,结果显示,二者的阳性检出率分别为30%(21/70)和34.29%(24/70),二者的总符合率为95.71%。本研究首次建立了基于MAP0827c蛋白的检测MAP抗体的间接ELISA方法,为MAP流行病学调查和检测提供了可行技术手段。 展开更多
关键词 副结核分枝杆菌 map0827c蛋白 原核表达 抗体 间接ELISA
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磁共振T2-mapping成像技术在膝关节骨性关节炎诊断中应用的研究进展
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作者 陈思 《黑龙江医学》 2025年第16期2042-2044,2048,共4页
T2-mapping成像技术通过分析软骨的T2弛豫时间,定量评估软骨组织学结构,揭示软骨中水含量和胶原纤维排列的变化,适用于早期检测软骨损伤和监测疾病进展,对评估软骨健康状况至关重要。T2-mapping成像技术不仅对膝关节骨性关节炎(KOA)的... T2-mapping成像技术通过分析软骨的T2弛豫时间,定量评估软骨组织学结构,揭示软骨中水含量和胶原纤维排列的变化,适用于早期检测软骨损伤和监测疾病进展,对评估软骨健康状况至关重要。T2-mapping成像技术不仅对膝关节骨性关节炎(KOA)的早期诊断和进展监测具有重要价值,还能在软骨修复过程中监测和评估软骨的生化变化。随着成像技术和数据处理能力的进一步发展,T2-mapping成像有望提高诊断精度、优化治疗方案,为KOA的管理和治疗带来革新。文章综述了磁共振成像技术(MRI)中的T2-mapping成像技术在KOA诊断和评估中的应用。 展开更多
关键词 磁共振T2-mapping成像 膝关节 骨性关节炎
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基于T_(2)mapping及超短回波时间技术定量探究早期膝骨关节炎的发病机制 被引量:1
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作者 牛飞格 孟祥虹 +1 位作者 孙曼 王植 《临床放射学杂志》 北大核心 2025年第2期350-356,共7页
目的基于浅表软骨的T_(2)mapping和骨软骨交界区(OCJ)的超短回波时间(UTE)T_(2)^(*)mapping探究膝骨关节炎(KOA)早期浅表软骨和骨软骨交界区的病变顺序。方法对纳入的30个健康膝和30个早期骨性关节炎膝进行常规和定量MRI扫描,主要扫描... 目的基于浅表软骨的T_(2)mapping和骨软骨交界区(OCJ)的超短回波时间(UTE)T_(2)^(*)mapping探究膝骨关节炎(KOA)早期浅表软骨和骨软骨交界区的病变顺序。方法对纳入的30个健康膝和30个早期骨性关节炎膝进行常规和定量MRI扫描,主要扫描序列包括三维水激发序列(3D WATS)、T_(2)mapping和UTE T_(2)^(*)mapping。将每个膝关节都分为股骨内侧髁、股骨外侧髁、胫骨内侧髁、胫骨外侧髁、髌骨和滑车区6个区域并测量每个区域的浅层软骨T_(2)值和OCJ T_(2)^(*)值。用独立样本t检验比较健康组和早期KOA组间的浅层软骨T_(2)值及OCJ T_(2)^(*)值。用Spearman相关性检验评估浅层软骨T_(2)值与OCJ T_(2)^(*)值间的相关性。结果早期KOA组的浅层软骨T_(2)值在股骨内侧髁(P<0.001)、胫骨内侧平台(P<0.001)、髌骨(P<0.001)和股骨滑车区(P=0.01)相对于健康组显著升高。早期KOA组OCJ T_(2)^(*)值在股骨内侧髁(P<0.001)、胫骨内侧平台(P<0.001)、胫骨外侧平台(P<0.001)、髌骨(P=0.001)和滑车区(P<0.001)均显著升高。浅层软骨T_(2)值和骨软骨交界区T_(2)^(*)值间存在正相关关系(r=0.37,P<0.001)。结论浅表软骨的T_(2)mapping和骨软骨交界区的UTE T_(2)^(*)mapping成像都可定量且无创识别早期KOA。在KOA早期,浅表软骨和OCJ都出现了生化改变。 展开更多
关键词 骨性关节炎 关节软骨骨软骨交界区 T_(2)mapping T_(2)^(*)mapping
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A modified method of discontinuity trace mapping using three-dimensional point clouds of rock mass surfaces 被引量:14
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作者 Keshen Zhang Wei Wu +3 位作者 Hehua Zhu Lianyang Zhang Xiaojun Li Hong Zhang 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2020年第3期571-586,共16页
This paper presents an automated method for discontinuity trace mapping using three-dimensional point clouds of rock mass surfaces.Specifically,the method consists of five steps:(1)detection of trace feature points by... This paper presents an automated method for discontinuity trace mapping using three-dimensional point clouds of rock mass surfaces.Specifically,the method consists of five steps:(1)detection of trace feature points by normal tensor voting theory,(2)co ntraction of trace feature points,(3)connection of trace feature points,(4)linearization of trace segments,and(5)connection of trace segments.A sensitivity analysis was then conducted to identify the optimal parameters of the proposed method.Three field cases,a natural rock mass outcrop and two excavated rock tunnel surfaces,were analyzed using the proposed method to evaluate its validity and efficiency.The results show that the proposed method is more efficient and accurate than the traditional trace mapping method,and the efficiency enhancement is more robust as the number of feature points increases. 展开更多
关键词 Rock mass DISCONTINUITY three-dimensional point clouds Trace mapping
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牦牛MAP3K7基因克隆及其与皮下前体脂肪细胞分化关系分析
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作者 蓝湘一 何洋洋 +5 位作者 张明 黄慈 王海波 王会 柴志欣 钟金城 《华北农学报》 北大核心 2025年第3期225-233,共9页
MAP3K7是MAP3K家族中的一员,在脂肪分化、炎症和肿瘤发生过程中发挥着重要作用,但其与牦牛脂肪分化的关系未见报道,并且其基因序列未知,制约了相关功能研究。为了获得牦牛MAP3K7基因编码区(CDS)保守区域序列,明确其表达特征,了解其在牦... MAP3K7是MAP3K家族中的一员,在脂肪分化、炎症和肿瘤发生过程中发挥着重要作用,但其与牦牛脂肪分化的关系未见报道,并且其基因序列未知,制约了相关功能研究。为了获得牦牛MAP3K7基因编码区(CDS)保守区域序列,明确其表达特征,了解其在牦牛不同组织中的表达水平及其与牦牛皮下前体脂肪细胞分化的相关性,利用RT-PCR技术,克隆牦牛MAP3K7基因并利用生物信息学方法分析生物学特性;利用实时荧光定量PCR(qPCR)技术检测MAP3K7在牦牛心、肝、脾等10个组织中的表达情况,与不同分化阶段牦牛皮下脂肪细胞中MAP3K7基因和脂分化相关基因的表达情况,同时结合油红O染色,明确MAP3K7基因对牦牛皮下前体脂肪细胞分化的影响。结果表明:克隆所得牦牛MAP3K7基因CDS区长度为1740 bp,编码579个氨基酸,与野牦牛MAP3K7预测序列CDS区比对发现第967位A>G,导致第323位氨基酸Thr>Ala的突变。理化性质分析发现,该蛋白为整体带负电的亲水酸性蛋白,具有112个氨基酸磷酸化位点,其氨基酸组成中,占比最高的是丝氨酸;二级结构预测显示,其无规卷曲含量最高,可能与TAB3等蛋白质间存在相互作用关系。核苷酸同源性比对与进化树结果表明,牦牛MAP3K7在反刍动物间具有较高的保守性,与野牦牛同源性最高且进化关系最近。组织定量检测发现,MAP3K7在牦牛皮下脂肪和内脏脂肪中的表达量相对较低,在牦牛皮下前体脂肪细胞诱导分化过程中,MAP3K7表达量随着成熟脂肪细胞的增多先上调,至分化到一定程度后下调。综上,MAP3K7基因在牦牛皮下前体脂肪细胞分化前期高表达以促进分化,但当分化到一定程度时,表达下调抑制进一步分化。 展开更多
关键词 牦牛 map3K7 基因表达 皮下脂肪 脂肪分化
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基于T2^(*)mapping的业余马拉松运动员足踝部关节软骨损伤及其影响因素分析 被引量:1
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作者 高雅丽 兰晓川 +2 位作者 王舒 宋凌恒 陈伟 《磁共振成像》 北大核心 2025年第2期94-99,共6页
目的基于T2^(*)mapping定量分析业余马拉松运动员足踝部关节软骨的T2^(*)值,并分析其与性别、年龄、身体质量指数(body mass index,BMI)、跑龄、跑量之间的相关性。材料与方法于2023年7月份至2023年9月份招募重庆市长跑运动爱好者48名,... 目的基于T2^(*)mapping定量分析业余马拉松运动员足踝部关节软骨的T2^(*)值,并分析其与性别、年龄、身体质量指数(body mass index,BMI)、跑龄、跑量之间的相关性。材料与方法于2023年7月份至2023年9月份招募重庆市长跑运动爱好者48名,其中跑量<300 km/月的36例(中低跑量组),跑量≥300 km/月的12例(高跑量组)。所有受试者均进行单侧无症状踝关节的MRI扫描,扫描序列包括T2^(*)mapping多回波自旋回波(spin echo,SE)序列矢状位、质子密度加权成像脂肪抑制(proton density-weighted imaging fat-saturated,PDWI-FS)序列矢状位、冠状位、横轴位以及T1加权脂肪抑制成像(T1-weighted imaging fat-saturated,T1WI-FS)序列横轴位。沿关节软骨轮廓边缘勾画距骨穹窿、跟骰关节跟骨面、骰骨面及后距下关节跟骨面、距骨面软骨作为感兴趣区(region of interest,ROI),获得相应的T2^(*)值。采用线性回归分析软骨T2^(*)值与年龄、BMI、跑龄的相关性,采用独立样本t检验分析不同跑量及不同性别间的软骨T2^(*)值差异。结果(1)距骨穹窿、跟骰关节跟骨面及骰骨面、后距下关节跟骨面及距骨面软骨T2^(*)值在性别上的差异均具有统计学意义(P=0.001、P<0.001、P=0.002、P=0.008、P=0.004);(2)高跑量组的距骨穹窿、后距下关节跟骨面软骨T2^(*)值高于中低跑量组(P=0.014、0.023),不同跑量的跟骰关节跟骨面及骰骨面、后距下关节距骨面软骨T2^(*)值的差异均无统计学意义(P=0.987、0.072、0.724);(3)距骨穹窿、跟骰关节跟骨面及骰骨面、后距下关节跟骨面、距骨面软骨T2^(*)值均与BMI呈正相关(r=0.376、0.384、0.300、0.422、0.455,P=0.005、0.004、0.019、0.001、0.001)。结论在业余马拉松运动员这一跑步群体中,与中低跑量相比,高跑量更有可能导致距骨穹窿、后距下关节跟骨面软骨损伤;而与较低的BMI相比,高BMI增加了距骨穹窿、跟骰关节跟骨面、骰骨面及后距下关节跟骨面、距骨面软骨损伤的风险。 展开更多
关键词 业余马拉松 跑步 软骨 磁共振成像 T2^(*)mapping
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湖羊MAP2K6基因多态性及其与生长性状的关联分析
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作者 朱硕 孙敬华 +9 位作者 鲍晶晶 尚明玉 刘天义 熊金珂 杨培福 姚书海 孙歆恬 贺建宁 杨会国 张莉 《中国畜牧兽医》 北大核心 2025年第8期3695-3706,共12页
【目的】探究丝裂原活化蛋白激酶激酶6(mitogen-activated protein kinase kinase6,MAP2K6)基因在湖羊不同发育阶段背最长肌组织中的表达水平,分析该基因的多态性与湖羊生长性状之间的相关性,以期为湖羊的生长性状分子育种提供新的标记... 【目的】探究丝裂原活化蛋白激酶激酶6(mitogen-activated protein kinase kinase6,MAP2K6)基因在湖羊不同发育阶段背最长肌组织中的表达水平,分析该基因的多态性与湖羊生长性状之间的相关性,以期为湖羊的生长性状分子育种提供新的标记资源。【方法】利用实时荧光定量PCR检测MAP2K6基因在湖羊(n=15)不同发育阶段背最长肌组织中的表达情况;通过Illumina OvineSNP 50K BeadChip检测湖羊(n=3024)MAP2K6基因的单核苷酸多态性(SNP),利用一般线性模型分析MAP2K6基因SNP位点与湖羊(n=1974)生长性状间的关联性,并使用R语言corrplot包计算湖羊体重与各体尺指标的相关系数。【结果】实时荧光定量PCR检测结果显示,湖羊背最长肌组织中MAP2K6基因表达量在初生到4月龄阶段逐渐升高,且3、4月龄的表达量均极显著高于初生、45日龄和6月龄(P<0.01)。湖羊MAP2K6基因中共检测到2个位点:rs414959578G>A和rs426057803A>G。关联分析结果显示,MAP2K6基因rs414959578G>A位点对湖羊5月龄体重、体高、体斜长、胸围、胸深、胸宽、十字部高、腰角宽,以及6月龄胸围、背膘厚有显著或极显著影响(P<0.05;P<0.01);rs426057803A>G位点对湖羊3月龄管围,5月龄胸围、管围和十字部高以及6月龄背膘厚有显著或极显著影响(P<0.05;P<0.01)。相关性分析结果显示,湖羊体重与体尺指标间存在显著正相关(P<0.05),但6月龄湖羊体斜长与6月龄胸宽、腰角宽,5月龄管围与6月龄腰角宽均不存在显著相关(P>0.05)。【结论】MAP2K6基因与湖羊背最长肌的发育相关,rs414959578G>A和rs426057803A>G位点对湖羊生长性状有显著影响。研究结果可为湖羊生长性状分子标记的挖掘和利用提供一定的理论依据。 展开更多
关键词 湖羊 map2K6基因 生长性状 关联分析
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