The objective of this research is to study the dynamic response characteristics of a three-beam system with intermediate elastic connections under a moving load/mass-spring.In this study,the finite Sine-Fourier transf...The objective of this research is to study the dynamic response characteristics of a three-beam system with intermediate elastic connections under a moving load/mass-spring.In this study,the finite Sine-Fourier transform was performed for the dynamic partial differential equations of a simply supported three-beam system(SSTBS)under a moving load and a moving mass-spring,respectively.The dynamic partial differential equations were transformed into dynamic ordinary differential equations relative to the time coordinates,and the equations were solved and the displacement Fourier amplitude spectral expressions were obtained.Finally,based on finite Sine-Fourier inverse transform,the expressions for dynamic response of SSTBS under the moving load and moving mass-spring were obtained.The proposed method,along with ANSYS,was used to calculate the dynamic response of the SSTBS under a moving load/mass-spring at different speeds.The results obtained herein were consistent with the ANSYS numerical calculation results,verifying the accuracy of the proposed method.The influence of the load/mass-spring’s moving speed on the dynamic deflections of SSTBS were analyzed.SSTBS has several critical speeds under a moving load/mass-spring.The vertical acceleration incurred by a change in the vertical speed of SSTBS due to the movement of mass-spring and the centrifugal acceleration produced by the movement of massspring on the vertical curve generated by SSTBS vibration could not be neglected.展开更多
Structured illumination microscopy(SIM)is a rapidly developing super-resolution technology.It has been widely used in various application fields of biomedicine due to its excellent two-and three-dimensional imaging ca...Structured illumination microscopy(SIM)is a rapidly developing super-resolution technology.It has been widely used in various application fields of biomedicine due to its excellent two-and three-dimensional imaging capabilities.Furthermore,faster three-dimensional imaging methods are required to help enable more research-oriented living cell imaging.In this paper,a fast and sensitive three-dimensional structured illumination microscopy based on asymmetric three-beam interference is proposed.An innovative time-series acquisition method is employed to halve the time required to obtain each raw image.A segmented half-wave plate as a substantial linear polarization modulation method is applied to the three-dimensional SIM system for the first time.Although it needs to acquire 21 raw images instead of 15 to reconstruct one super-resolution image,the SIM setup proposed in this paper is 30%faster than the traditional spatial light modulator-SIM(SLM-SIM)in imaging each super-resolution image.The related theoretical derivation,hardware system,and verification experiment are elaborated in this paper.The stable and fast 3D super-resolution imaging method proposed in this paper is of great significance to the research of organelle interaction,intercellular communication,and other biomedical fields.展开更多
基金The Fundamental Research Funds for the Central Universities of Central South University under Grant No.2018zzts189the National Natural Science Foundations of China under Grant Nos.51408449 and 51778630the Innovation-driven Plan in Central South University under Grant No.2015CX006。
文摘The objective of this research is to study the dynamic response characteristics of a three-beam system with intermediate elastic connections under a moving load/mass-spring.In this study,the finite Sine-Fourier transform was performed for the dynamic partial differential equations of a simply supported three-beam system(SSTBS)under a moving load and a moving mass-spring,respectively.The dynamic partial differential equations were transformed into dynamic ordinary differential equations relative to the time coordinates,and the equations were solved and the displacement Fourier amplitude spectral expressions were obtained.Finally,based on finite Sine-Fourier inverse transform,the expressions for dynamic response of SSTBS under the moving load and moving mass-spring were obtained.The proposed method,along with ANSYS,was used to calculate the dynamic response of the SSTBS under a moving load/mass-spring at different speeds.The results obtained herein were consistent with the ANSYS numerical calculation results,verifying the accuracy of the proposed method.The influence of the load/mass-spring’s moving speed on the dynamic deflections of SSTBS were analyzed.SSTBS has several critical speeds under a moving load/mass-spring.The vertical acceleration incurred by a change in the vertical speed of SSTBS due to the movement of mass-spring and the centrifugal acceleration produced by the movement of massspring on the vertical curve generated by SSTBS vibration could not be neglected.
基金This work was funded by The National Key R&D Program of China(2016YFF0102000)Scientific Research and Equipment Development Project of CAS(YJKYYQ20180032 and YJKYYQ20190048)Major Innovative Research Team of Suzhou(ZXT2019007).
文摘Structured illumination microscopy(SIM)is a rapidly developing super-resolution technology.It has been widely used in various application fields of biomedicine due to its excellent two-and three-dimensional imaging capabilities.Furthermore,faster three-dimensional imaging methods are required to help enable more research-oriented living cell imaging.In this paper,a fast and sensitive three-dimensional structured illumination microscopy based on asymmetric three-beam interference is proposed.An innovative time-series acquisition method is employed to halve the time required to obtain each raw image.A segmented half-wave plate as a substantial linear polarization modulation method is applied to the three-dimensional SIM system for the first time.Although it needs to acquire 21 raw images instead of 15 to reconstruct one super-resolution image,the SIM setup proposed in this paper is 30%faster than the traditional spatial light modulator-SIM(SLM-SIM)in imaging each super-resolution image.The related theoretical derivation,hardware system,and verification experiment are elaborated in this paper.The stable and fast 3D super-resolution imaging method proposed in this paper is of great significance to the research of organelle interaction,intercellular communication,and other biomedical fields.
