Objective To explore the effect of acupuncture combined with medicated thread moxibustion of Zhuang medicine on insomnia rats. Methods Sixty SPF Wistar rats were randomly divided into four groups, including a normal c...Objective To explore the effect of acupuncture combined with medicated thread moxibustion of Zhuang medicine on insomnia rats. Methods Sixty SPF Wistar rats were randomly divided into four groups, including a normal control group （control group）, a sham operation group, a model group and a treatment group, with 15 rats in each group. In the model group and treatment group, insomnia model were established by injecting orexin into lateral ventricle of rats. In the sham operation group, artificial cerebrospinal fluid was injected into lateral ventricle of rats, and the rats in control group were not treated. On the basis of modeling, the rats in treatment group were treated by acupuncture combined with medicated thread moxibustion of Zhuang medicine which was conducted once a day, and the treatment lasted for 14 days. The expression of orexin mRNA and orexin content were detected, and the changes of associated receptors, including orexin 1 receptor （OX I R） and orexin 2 receptor （OX 2 R） were observed. Results The expression of orexin mRNA in model group （6.54 ± 1.21） significantly increased when compared with control group （0.66±0.12, P〈0.01）, and orexin content in model group [（i 156.35±126.88） pg/mL] significantly increased when compared with control group [（427.12_± 32.54） pg/mL, P〈O.OI]. The expression of orexin mRNA in treatment group （0.87 ± 0.26） significantly reduced when compared with the model group （P〈0.01）, and orexin content in the treatment group [（585.23 ± 89.21） pg/mL] significantly reduced when compared with the model group （P〈O.01）. The percentage of OX I R positive cells in the model group （0.64±0.15） significantly increased when compared with the control group （0.21±0.03, P〈0.01）, and the percentage of OX 2 R positive cells in the model group （0.76±0.18） significantly increased when compared with the control group （0.24±0.01, P〈O.01）. The percentage of OX 1 R positive cells in the treatment group （0.36_±0.08） significantly reduced when compared with the model group （P〈0.01）. The percentage of OX 2 R positive cells in treatment group （0.25_± 0.03） significantly reduced when compared with model group （P〈0.01）. Conclusion It might be an effective link of acupuncture combined with medicated thread moxibustion of Zhuang medicine in treatment of insomnia that the treatment could inhibit the awakening promotion effect of orexin by affecting orexin, OX I R and OX 2 R.展开更多
基金Supported by National Natural Science Foundation of China:81360579
文摘Objective To explore the effect of acupuncture combined with medicated thread moxibustion of Zhuang medicine on insomnia rats. Methods Sixty SPF Wistar rats were randomly divided into four groups, including a normal control group （control group）, a sham operation group, a model group and a treatment group, with 15 rats in each group. In the model group and treatment group, insomnia model were established by injecting orexin into lateral ventricle of rats. In the sham operation group, artificial cerebrospinal fluid was injected into lateral ventricle of rats, and the rats in control group were not treated. On the basis of modeling, the rats in treatment group were treated by acupuncture combined with medicated thread moxibustion of Zhuang medicine which was conducted once a day, and the treatment lasted for 14 days. The expression of orexin mRNA and orexin content were detected, and the changes of associated receptors, including orexin 1 receptor （OX I R） and orexin 2 receptor （OX 2 R） were observed. Results The expression of orexin mRNA in model group （6.54 ± 1.21） significantly increased when compared with control group （0.66±0.12, P〈0.01）, and orexin content in model group [（i 156.35±126.88） pg/mL] significantly increased when compared with control group [（427.12_± 32.54） pg/mL, P〈O.OI]. The expression of orexin mRNA in treatment group （0.87 ± 0.26） significantly reduced when compared with the model group （P〈0.01）, and orexin content in the treatment group [（585.23 ± 89.21） pg/mL] significantly reduced when compared with the model group （P〈O.01）. The percentage of OX I R positive cells in the model group （0.64±0.15） significantly increased when compared with the control group （0.21±0.03, P〈0.01）, and the percentage of OX 2 R positive cells in the model group （0.76±0.18） significantly increased when compared with the control group （0.24±0.01, P〈O.01）. The percentage of OX 1 R positive cells in the treatment group （0.36_±0.08） significantly reduced when compared with the model group （P〈0.01）. The percentage of OX 2 R positive cells in treatment group （0.25_± 0.03） significantly reduced when compared with model group （P〈0.01）. Conclusion It might be an effective link of acupuncture combined with medicated thread moxibustion of Zhuang medicine in treatment of insomnia that the treatment could inhibit the awakening promotion effect of orexin by affecting orexin, OX I R and OX 2 R.
