The delivery efficiency of DNA nanoassemblies to the cytosol remains unsatisfactory due to endoand lysosomal entrapment and degradation,which restricts their bioanalytical and biomedical applications.Herein,the author...The delivery efficiency of DNA nanoassemblies to the cytosol remains unsatisfactory due to endoand lysosomal entrapment and degradation,which restricts their bioanalytical and biomedical applications.Herein,the authors developed a disulfide-containing molecular sticker(DSMS)assisted approach to achieve efficient cytosolic delivery of DNA nanoassemblies.DSMS is designed to consist of a disulfide unit for thiol-mediated uptake and a guanidinium cation unit for strongly adhering to DNA nanoassemblies.After attaching with DSMS,a set of DNA nanoassemblies maintained their original three-dimensional features but had a different cellular internalization pathway.These results demonstrated that DSMS-DNA nanoassemblies complex did not enter the cells via endocytosis but instead entered through thiol-mediated direct uptake.Taking advantages of this facile assembly,universal applicability,and direct cytosolic delivery,DSMS might serve as a potent agent to facilitate the applications of DNA nanoassemblies in a variety of fields,such as bioimaging,drug delivery,and gene therapy.展开更多
基金supported by the Natural Science Foundation of China(grant nos.91959102,21635002,and 61805041)the Natural Science Foundation of Fujian Province of China(grant no.2017J06004)+1 种基金the Shanghai Rising-Star Program(grant no.19QA1405400)the Program for Changjiang Scholars and Innovative Research Team in University(grant no.IRT15R11).
文摘The delivery efficiency of DNA nanoassemblies to the cytosol remains unsatisfactory due to endoand lysosomal entrapment and degradation,which restricts their bioanalytical and biomedical applications.Herein,the authors developed a disulfide-containing molecular sticker(DSMS)assisted approach to achieve efficient cytosolic delivery of DNA nanoassemblies.DSMS is designed to consist of a disulfide unit for thiol-mediated uptake and a guanidinium cation unit for strongly adhering to DNA nanoassemblies.After attaching with DSMS,a set of DNA nanoassemblies maintained their original three-dimensional features but had a different cellular internalization pathway.These results demonstrated that DSMS-DNA nanoassemblies complex did not enter the cells via endocytosis but instead entered through thiol-mediated direct uptake.Taking advantages of this facile assembly,universal applicability,and direct cytosolic delivery,DSMS might serve as a potent agent to facilitate the applications of DNA nanoassemblies in a variety of fields,such as bioimaging,drug delivery,and gene therapy.
