This study attempted to assess the lethal concentration(96-h LC_(50))effects of imidacloprid(neonicotinoid pesticide),thiamethoxam(neonicotinoid pesticide),and their combination on juvenile Zebrafish(Danio rerio).Each...This study attempted to assess the lethal concentration(96-h LC_(50))effects of imidacloprid(neonicotinoid pesticide),thiamethoxam(neonicotinoid pesticide),and their combination on juvenile Zebrafish(Danio rerio).Each set of trials contained a control(de-chlorinated tap water),and the experiments were repeated three times.The fish(n=10)were randomly measured with an average length of(3.4±0.34)cm and weight of(1±0.1)g.The temperature was kept at 24℃.Experiments 1 and 2 were designed to investigate at the acute toxicity of imidacloprid and thiamethoxam on juvenile zebrafish(Danio rerio)respectively,whereas experiment 3 was aimed at the combined toxicity of IMI and THM on zebrafish.The tests followed the same study design,and each experiment used seven different logarithmic concentrations of imidacloprid insecticides(310.00,317.08,324.33,331.74,339.32,347.07,355.00 mg/L)and thiamethoxam(175.00,185.52,200.93,215.30,230.70,247.20,264.88 mg/L).The results show that THM is more toxic than IMI,with LC_(50)values of 190.34 mg/L for THM and 310.92 mg/L for IMI.Both individual toxicities showed a substantial positive connection(P<0.05)with confidence limits of 321.50-300.68 mg/L for IMI and 199.91-181.21 mg/L for THM.The joint toxicity test was carried out using the 96-h LC_(50)values of imidacloprid and thiamethoxam obtained in the individual acute toxicity trials at a 1:1 ratio.The Additive Index(AI)demonstrated that imidacloprid and thiamethoxam acted synergistically on D.rerio.As a matter of fact,more research is needed to better understand the impact of IMI and THM on other aquatic organisms and also create strategies to mitigate its harmful effects on aquatic life.展开更多
The prevention and control effect of 35% thiamethoxam-prochloraz FS to rice thrips and rice bakanae disease and its influence on the quality of rice seedlings were studied. The results show that when 100 kg of rice se...The prevention and control effect of 35% thiamethoxam-prochloraz FS to rice thrips and rice bakanae disease and its influence on the quality of rice seedlings were studied. The results show that when 100 kg of rice seeds were coated with 200 g of 35% thiamethoxam-prochloraz FS,the control effects to rice thrips were 93.0% and 84.7% 15 and 20 days after sowing, and the control effect to rice bakanae disease was 90.8% 30 days after sowing. As 100 kg of rice seeds were coated with 250 g of 35% thiamethoxam-prochloraz FS, the control effects to rice thrips were 98.2% and 94.9% 15 and 20 days after sowing, and the control ef- fect to rice bakanae disease was 94.6% 30 days after sowing. 35% thiamethoxam- prochloraz FS is safe to rice seedlings and can effectively promote the growth of rice seedlings.展开更多
Seed treatments with the neonicotinoid insecticides imidacloprid and thiamethoxam were evaluated to determine whether the chemicals at effective concentrations for aphid control would influence the germination and ear...Seed treatments with the neonicotinoid insecticides imidacloprid and thiamethoxam were evaluated to determine whether the chemicals at effective concentrations for aphid control would influence the germination and early growth of oilseed rape,Brassica napus.Treatment with imidacloprid or thiamethoxam did not affect the cumulative germination rate,but significantly inhibited establishment potential by suppressing root system development in the cotyledon stage.However,these alterations in seedling development in the thiamethoxam-treated seeds appeared not to be detrimental as leaves developed;in contrast,for the seedlings with imidacloprid as seed treatment agent,a significantly decreased shoot/root ratio was still evident at the late two-leaf stage.After two leaves developed,chlorophyll content per leaf in the thiamethoxam treatment was significantly higher than that of the control,while chlorophyll content per leaf in the imidacloprid treatment remained close to that in the control.Most other parameters,i.e.,height,leaf area,weight of stem,leaf or root,and other growth indexes,between the treatments and the control showed no significant difference.Additionally,it was found that storage time of the treated seeds had a significant effect on cumulative germination rate.Treatment 30 d before planting significantly reduced germination relative to that of the control.All of the plants treated with neonicotinoids were shown to have significant anti-aphid characteristics that persisted until the end of the trial.展开更多
[Objective] The paper was to provide scientific basis for popularization and application of 70% Thiamethoxam ZF. [Method] 70% Thiamethoxam ZF was selected and applied in the soil for controlling Ceratovacuna lanigera ...[Objective] The paper was to provide scientific basis for popularization and application of 70% Thiamethoxam ZF. [Method] 70% Thiamethoxam ZF was selected and applied in the soil for controlling Ceratovacuna lanigera Zehntner and Baliathrips serratus Kobus. [ Result ] 70% Thiamethoxam ZF had good con-rol effect against C. lanigera and B. serratus. The optimum dosage of 70% Thiamethoxam ZF was 450 g/hm^2 （effective ingredients 315 g）. 70% Thiamethoxam ZF （ dosage per hm^2 ） mixed with the regular fertilizer （ dosage per hm^2 ） should be applied together in sugarcane planting and management from February to June. The control effects against C. lanigera and B. serratus were above 98.4% and 82.9% respectively. The actual yield and sugar content were increased by more than 34 290 kg/hm^2 and 6.8% respectively compared to blank control. [ Conclusion] 70% Thiamethoxam ZF was a new ideal pesticide with characteristics of sustained- release, long-lasting and low-toxic for controlling C. lanigera and B. serratus, and should be applied widely in the sugarcane planting area.展开更多
[Objectives]The paper was to verify the field efficacy of 1%bifenthrin·thiamethoxam GR replacing organophosphorus GR against sweet potato weevil and its impact on the yield and quality of sweet potato.[Methods]A ...[Objectives]The paper was to verify the field efficacy of 1%bifenthrin·thiamethoxam GR replacing organophosphorus GR against sweet potato weevil and its impact on the yield and quality of sweet potato.[Methods]A total of 4 field trials were conducted in Guangdong Province,including 1%bifenthrin·thiamethoxam GR applied at the doses of 3,4 and 5 kg/667 m^(2),and 3%phoxim GR applied at the dose of 4 kg/667 m^(2).[Results]1%Bifenthrin·thiamethoxam GR applied at the dose of 5 kg/667 m^(2)had excellent control effects on sweet potato weevil,with an average control effect of 77.60%,which was significantly higher than that of 3%phoxim GR applied at the dose of 4 kg/667 m^(2)(48.52%).And the average yield increase rate of sweet potato treated with 1%bifenthrin·thiamethoxam GR reached 24.79%,significantly higher than 12.37%in the control group.[Conclusions]1%Bifenthrin·thiamethoxam GR should be evenly distributed on the ridge surface near sweet potato within 5-7 d after planting,and the recommended dosage is 5 kg/667 m^(2),which will have good control effect on sweet potato weevil and increase the yield of sweet potato.展开更多
The linearity, stability, accuracy and inter-day precisions of the assay method were evaluated in water, soil and rhizomes and leaves of Hedychium coronarium of the Zingiberaceae family. Mato Grosso do Sul is a predom...The linearity, stability, accuracy and inter-day precisions of the assay method were evaluated in water, soil and rhizomes and leaves of Hedychium coronarium of the Zingiberaceae family. Mato Grosso do Sul is a predominantly agricultural state in Brazil and has many rivers and permanent and seasonal lakes, in which the Hedychium coronarium, an aquatic plant, is found the swampy environments. The prepared samples were analyzed quantitatively by High Performance Liquid Chromatography with PDA and UV detection for the presence of thiamethoxam. The thiamethoxam was recovered from these samples at rates ranging from 81.16% - 99.93%. The coefficient of variation in the quantitative analysis of the thiamethoxam was under 5%. The linearity of the method was determined by linear regression. The analysis of the samples spiked with known amounts of analyte demonstrated that the response was proportional to the concentrations of the samples with determination coefficients of r2 = 0.9992 (water and soil) and r2 = 0.9990 (leaves and rhizomes) for the linear range of the analytical calibration curves of the samples. The detection limit was 0.36 μg.L-1 and quantification limit was 1.2 μg.L-1 for thiamethoxam. The method was considered sensitive for quantification of the thiamethoxam in water, soil and rhizomes and leaves of Hedychium coronarium.展开更多
基金Supported by the Central Public-interest Scientific Institution Basal Research Fund,CAFS(2025XT0902)the earmarked fund for China Agriculture Research System(CARS-46).
文摘This study attempted to assess the lethal concentration(96-h LC_(50))effects of imidacloprid(neonicotinoid pesticide),thiamethoxam(neonicotinoid pesticide),and their combination on juvenile Zebrafish(Danio rerio).Each set of trials contained a control(de-chlorinated tap water),and the experiments were repeated three times.The fish(n=10)were randomly measured with an average length of(3.4±0.34)cm and weight of(1±0.1)g.The temperature was kept at 24℃.Experiments 1 and 2 were designed to investigate at the acute toxicity of imidacloprid and thiamethoxam on juvenile zebrafish(Danio rerio)respectively,whereas experiment 3 was aimed at the combined toxicity of IMI and THM on zebrafish.The tests followed the same study design,and each experiment used seven different logarithmic concentrations of imidacloprid insecticides(310.00,317.08,324.33,331.74,339.32,347.07,355.00 mg/L)and thiamethoxam(175.00,185.52,200.93,215.30,230.70,247.20,264.88 mg/L).The results show that THM is more toxic than IMI,with LC_(50)values of 190.34 mg/L for THM and 310.92 mg/L for IMI.Both individual toxicities showed a substantial positive connection(P<0.05)with confidence limits of 321.50-300.68 mg/L for IMI and 199.91-181.21 mg/L for THM.The joint toxicity test was carried out using the 96-h LC_(50)values of imidacloprid and thiamethoxam obtained in the individual acute toxicity trials at a 1:1 ratio.The Additive Index(AI)demonstrated that imidacloprid and thiamethoxam acted synergistically on D.rerio.As a matter of fact,more research is needed to better understand the impact of IMI and THM on other aquatic organisms and also create strategies to mitigate its harmful effects on aquatic life.
基金Supported by the Guiding Plan for Agricultural Science and Technology of Yancheng City(YKN2013018)~~
文摘The prevention and control effect of 35% thiamethoxam-prochloraz FS to rice thrips and rice bakanae disease and its influence on the quality of rice seedlings were studied. The results show that when 100 kg of rice seeds were coated with 200 g of 35% thiamethoxam-prochloraz FS,the control effects to rice thrips were 93.0% and 84.7% 15 and 20 days after sowing, and the control effect to rice bakanae disease was 90.8% 30 days after sowing. As 100 kg of rice seeds were coated with 250 g of 35% thiamethoxam-prochloraz FS, the control effects to rice thrips were 98.2% and 94.9% 15 and 20 days after sowing, and the control ef- fect to rice bakanae disease was 94.6% 30 days after sowing. 35% thiamethoxam- prochloraz FS is safe to rice seedlings and can effectively promote the growth of rice seedlings.
基金supported by the National Special Fund for Agro-Scientific Research of Public Interests of China(201303030)the Anhui Provincial Natural Science Foundation,China(1408085MKL65)
文摘Seed treatments with the neonicotinoid insecticides imidacloprid and thiamethoxam were evaluated to determine whether the chemicals at effective concentrations for aphid control would influence the germination and early growth of oilseed rape,Brassica napus.Treatment with imidacloprid or thiamethoxam did not affect the cumulative germination rate,but significantly inhibited establishment potential by suppressing root system development in the cotyledon stage.However,these alterations in seedling development in the thiamethoxam-treated seeds appeared not to be detrimental as leaves developed;in contrast,for the seedlings with imidacloprid as seed treatment agent,a significantly decreased shoot/root ratio was still evident at the late two-leaf stage.After two leaves developed,chlorophyll content per leaf in the thiamethoxam treatment was significantly higher than that of the control,while chlorophyll content per leaf in the imidacloprid treatment remained close to that in the control.Most other parameters,i.e.,height,leaf area,weight of stem,leaf or root,and other growth indexes,between the treatments and the control showed no significant difference.Additionally,it was found that storage time of the treated seeds had a significant effect on cumulative germination rate.Treatment 30 d before planting significantly reduced germination relative to that of the control.All of the plants treated with neonicotinoids were shown to have significant anti-aphid characteristics that persisted until the end of the trial.
基金Supported by Special Fund for China Agricultural Industry Research System(CARS-20-2-2)Special Fund for Agricultural Industry Research System of Yunnan Province(YNGZTX-4-92)
文摘[Objective] The paper was to provide scientific basis for popularization and application of 70% Thiamethoxam ZF. [Method] 70% Thiamethoxam ZF was selected and applied in the soil for controlling Ceratovacuna lanigera Zehntner and Baliathrips serratus Kobus. [ Result ] 70% Thiamethoxam ZF had good con-rol effect against C. lanigera and B. serratus. The optimum dosage of 70% Thiamethoxam ZF was 450 g/hm^2 （effective ingredients 315 g）. 70% Thiamethoxam ZF （ dosage per hm^2 ） mixed with the regular fertilizer （ dosage per hm^2 ） should be applied together in sugarcane planting and management from February to June. The control effects against C. lanigera and B. serratus were above 98.4% and 82.9% respectively. The actual yield and sugar content were increased by more than 34 290 kg/hm^2 and 6.8% respectively compared to blank control. [ Conclusion] 70% Thiamethoxam ZF was a new ideal pesticide with characteristics of sustained- release, long-lasting and low-toxic for controlling C. lanigera and B. serratus, and should be applied widely in the sugarcane planting area.
文摘[Objectives]The paper was to verify the field efficacy of 1%bifenthrin·thiamethoxam GR replacing organophosphorus GR against sweet potato weevil and its impact on the yield and quality of sweet potato.[Methods]A total of 4 field trials were conducted in Guangdong Province,including 1%bifenthrin·thiamethoxam GR applied at the doses of 3,4 and 5 kg/667 m^(2),and 3%phoxim GR applied at the dose of 4 kg/667 m^(2).[Results]1%Bifenthrin·thiamethoxam GR applied at the dose of 5 kg/667 m^(2)had excellent control effects on sweet potato weevil,with an average control effect of 77.60%,which was significantly higher than that of 3%phoxim GR applied at the dose of 4 kg/667 m^(2)(48.52%).And the average yield increase rate of sweet potato treated with 1%bifenthrin·thiamethoxam GR reached 24.79%,significantly higher than 12.37%in the control group.[Conclusions]1%Bifenthrin·thiamethoxam GR should be evenly distributed on the ridge surface near sweet potato within 5-7 d after planting,and the recommended dosage is 5 kg/667 m^(2),which will have good control effect on sweet potato weevil and increase the yield of sweet potato.
文摘The linearity, stability, accuracy and inter-day precisions of the assay method were evaluated in water, soil and rhizomes and leaves of Hedychium coronarium of the Zingiberaceae family. Mato Grosso do Sul is a predominantly agricultural state in Brazil and has many rivers and permanent and seasonal lakes, in which the Hedychium coronarium, an aquatic plant, is found the swampy environments. The prepared samples were analyzed quantitatively by High Performance Liquid Chromatography with PDA and UV detection for the presence of thiamethoxam. The thiamethoxam was recovered from these samples at rates ranging from 81.16% - 99.93%. The coefficient of variation in the quantitative analysis of the thiamethoxam was under 5%. The linearity of the method was determined by linear regression. The analysis of the samples spiked with known amounts of analyte demonstrated that the response was proportional to the concentrations of the samples with determination coefficients of r2 = 0.9992 (water and soil) and r2 = 0.9990 (leaves and rhizomes) for the linear range of the analytical calibration curves of the samples. The detection limit was 0.36 μg.L-1 and quantification limit was 1.2 μg.L-1 for thiamethoxam. The method was considered sensitive for quantification of the thiamethoxam in water, soil and rhizomes and leaves of Hedychium coronarium.
