Ecological effects of crude oil residues on weed rhizospheres are still vague. The quantitative and diversity changes and metabolic responses of soil-bacterial communities in common dandelion （Taraxacum officinale）,...Ecological effects of crude oil residues on weed rhizospheres are still vague. The quantitative and diversity changes and metabolic responses of soil-bacterial communities in common dandelion （Taraxacum officinale）, jerusalem artichoke （Silphiurn perfoliatum L.） and evening primrose （A colypha australis L.） rhizospheric soils were thus examined using the method of carbon source utilization. The results indicated that there were various toxic effects of crude oil residues on the growth and reproduction of soil bacteria, but the weed rhizospheres could mitigate the toxic effects. Total heterotrophic counting colony-forming units （CFUs） in the rhizospheric soils were significantly higher than those in the non-rhizospheric soils. The culturable soil-bacterial CFUs in the jerusalem artichoke （S. perfoliatum） rhizosphere polluted with 0.50 kg/pot of crude oil residues were almost twice as much as those with 0.25 kg/pot and without the addition of crude oil residues. The addition of crude oil residues increased the difference in substrate evenness, substrate richness, and substrate diversity between non-rhizospheric and rhizospheric soils of T. officinale and A. australis, but there was no significant （p〉0.05） difference in the Shannon＇s diversity index between non-rhizospheric and rhizospheric soils of S. perfoliatum. The rhizospheric response of weed species to crude oil residues suggested that S. perfoliatum may be a potential weed species for the effective plant-microorganism bioremediation of contaminated soils by crude oil residues.展开更多
In the present study, a biofertilizer on the basis of Streptomyces fumanus gn-2 was used for the treatment of wheat and soybean seeds (dose 104 spore/ml) before planting them in soil with low fertility in order to det...In the present study, a biofertilizer on the basis of Streptomyces fumanus gn-2 was used for the treatment of wheat and soybean seeds (dose 104 spore/ml) before planting them in soil with low fertility in order to determine the effect of this biological agent on germination rate;the growth of seedlings, shoots, and the maturation phase of plants;the rhizosphere’s functional biodiversity;and the resistance of these plants to pathogens. Seeds were soaked in the suspension for a period of two or three hours. During the growing season of the crop, no additional fertilizing and spraying of a biopesticide against diseases or pests occurred. Despite the soil having low fertility, low quantities of organic matter, and not having been before used for the cultivation of agricultural plants, this biofertilizer showed a strong stimulatory effect on the growth of seeds and seedlings of wheat and soybeans. The average germination and seed vigor increased by 1.5 - 2.0 times, and the phenophases were accelerated to three to five days. In all phases of vegetation, the ammonifying bacteria in the presence of an antagonist (a biological agent) developed rapidly and were constantly present in significant numbers in the rhizosphere. Streptomyces fumanus introduced into non-sterile soil entered into competition with the local soil microflora and had the ability to colonize the rhizosphere system of plants. The use of a formulation of Streptomyces gn-2 has improved the composition of rhizosphere microflora, attracting saprophytic microorganisms: ammonificators and oligotrophs. The presence of the biocontrol microorganism Streptomyces fumanus in the rhizosphere plays an important role in enhancing the growth and development of useful groups, such as nitrogen-fixing bacteria.展开更多
Since the early 2000s, many satellite passive microwave brightness temperature （BT） archives, such as the Advanced Microwave Scanning Radiometer for the Earth Observing System （AMSR-E） BTs, have become the useful ...Since the early 2000s, many satellite passive microwave brightness temperature （BT） archives, such as the Advanced Microwave Scanning Radiometer for the Earth Observing System （AMSR-E） BTs, have become the useful resources for assessing the changes in the surface and deep soil moistures over both arid and semi-arid regions. In this study, we used a new soil effective temperature （T scheme and the archived AMSR-E BTs to estimate surface soil moisture （SM） over the Nagqu region in the central Tibetan Plateau, China. The surface and deep soil temperatures required for the calculation of regional-scale T were obtained from outputs of the Community Land Model version 4.5 （CLM4.5）. In situ SM measurements at the CEOP-CAMP/Tibet （Coordinated Enhanced Observing Period Asia-Australia Monsoon Project on the Tibetan Plateau） experimental sites were used to validate the AMSR-E-based SM estimations at regional and single-site scales. Furthermore, the spatial distribution of monthly mean surface SM over the Nagqu region was obtained from 16 daytime AMSR-E BT observations in July 2004 over the Nagqu region. Results revealed that the AMSR-E-based surface SM estimations agreed well with the in situ-based surface SM measurements, with the root mean square error （RMSE） ranging from 0.042 to 0.066 m3/m3 and the coefficient of determination （R2） ranging from 0.71 to 0.92 during the nighttime and daytime. The regional surface soil water state map showed a clear spatial pattern related to the terrain. It indicated that the lower surface SM values occurred in the mountainous areas of the northern, mid-western and southeastern parts of Nagqu region, while the higher surface SM values appeared in the low elevation areas such as the Tongtian River Basin, Namco Lake and bog meadows in the central part of Nagqu region. Our analysis also showed that the new T^scheme does not require special fitting parameters or additional assumptions, which simplifies the data requirements for regional-scale applications. This scheme combined with the archived satellite passive microwave BT observations can be used to estimate the historical surface SM for hydrological process studies over the Tibetan Plateau regions.展开更多
The negative effect of soil erosion and soil compaction is well documented for the purpose of optimum rangeland functioning, while the impact of rangeland degradation on effective soil depth is seldom quantified. The ...The negative effect of soil erosion and soil compaction is well documented for the purpose of optimum rangeland functioning, while the impact of rangeland degradation on effective soil depth is seldom quantified. The aim of this study was to quantify the response of vegetation cover and soil properties, particularly effective soil depth and soil texture to rangeland degradation. Forty-one farms were sampled in the arid and semi-arid climate of South Africa. Within these farms, data was collected over a vegetation degradation gradient. Results showed a significant decline in relative basal cover (94% ± 15% to 39% ± 17%) and soil depth (90% ± 14% to 73% ± 24%) as rangeland degraded. Soil texture changes over the degradation gradients vary for different homogeneous vegetation types. Indications regarding the loss of a functioning rangeland ecosystem were also demonstrated, using objective long-term relations between rangeland conditions and grazing capacity. The study highlights the importance of sustainable rangeland management practices to reduce the loss in effective soil depth and to ensure the sustainable utilization of the rangeland ecosystem. These results can probably extrapolate to other arid and semi-arid rangelands worldwide.展开更多
A rhizobox system constructed with crude oil- contaminated soil was vegetated with alfalfa （Medicago sativa L.） to evaluate the rhizosphere effects on the soil microbial population and functional structure, and to e...A rhizobox system constructed with crude oil- contaminated soil was vegetated with alfalfa （Medicago sativa L.） to evaluate the rhizosphere effects on the soil microbial population and functional structure, and to explore the potential mechanisms by which plants enhance the removal of crude oil in soil. During the 80-day experiment, 31.6% of oil was removed from the adjacent rhizosphere （AR）; this value was 27% and 53% higher than the percentage of oil removed from the far rhizosphere （FR） and from the non-rhizosphere （NR）, respectively. The populations of heterotrophic bacteria and hydrocarbon- degrading bacteria were higher in the AR and FR than in the NR. However, the removal rate of crude oil was positively correlated with the proportion of hydrocarbon- degrading bacteria in the rhizosphere. In total, 796, 731, and 379 functional genes were detected by microarray in the AR, FR, and NR, respectively. Higher proportions of functional genes related to carbon degradation and organic remediation, were found in rhizosphere soil compared with NR soil, suggesting that the rhizosphere selectively increased the abundance of these specific functional genes. The increase in water-holding capacity and decrease in pH as well as salinity of the soil all followed the order of AR 〉 FR 〉 NR. Canonical component analysis showed that salinity was the most important environmental factor influencing the microbial functional structure in the rhizosphere and that salinity was negatively correlated with the abundance of carbon and organic degradation genes.展开更多
Heavy tamping is one of the important methods for ground treatment. But the vibration is often a problem that restricts the application during heavy tamping. On the other hand, as an instant impact load with controlla...Heavy tamping is one of the important methods for ground treatment. But the vibration is often a problem that restricts the application during heavy tamping. On the other hand, as an instant impact load with controllable energy, heavy tamping is a good test in situ for the study of soil dynamics. With the analysis in the field of frequency, deductive method for medium effecting function and excitation function of heavy tamping is put forward in this paper.展开更多
基金The National Natural Science Foundation of China as an Outstanding Youth Fund grant (No. 20225722) the National NaturalScience Foundation for the Joint China-Russia Project (No. 20611120015)
文摘Ecological effects of crude oil residues on weed rhizospheres are still vague. The quantitative and diversity changes and metabolic responses of soil-bacterial communities in common dandelion （Taraxacum officinale）, jerusalem artichoke （Silphiurn perfoliatum L.） and evening primrose （A colypha australis L.） rhizospheric soils were thus examined using the method of carbon source utilization. The results indicated that there were various toxic effects of crude oil residues on the growth and reproduction of soil bacteria, but the weed rhizospheres could mitigate the toxic effects. Total heterotrophic counting colony-forming units （CFUs） in the rhizospheric soils were significantly higher than those in the non-rhizospheric soils. The culturable soil-bacterial CFUs in the jerusalem artichoke （S. perfoliatum） rhizosphere polluted with 0.50 kg/pot of crude oil residues were almost twice as much as those with 0.25 kg/pot and without the addition of crude oil residues. The addition of crude oil residues increased the difference in substrate evenness, substrate richness, and substrate diversity between non-rhizospheric and rhizospheric soils of T. officinale and A. australis, but there was no significant （p〉0.05） difference in the Shannon＇s diversity index between non-rhizospheric and rhizospheric soils of S. perfoliatum. The rhizospheric response of weed species to crude oil residues suggested that S. perfoliatum may be a potential weed species for the effective plant-microorganism bioremediation of contaminated soils by crude oil residues.
文摘In the present study, a biofertilizer on the basis of Streptomyces fumanus gn-2 was used for the treatment of wheat and soybean seeds (dose 104 spore/ml) before planting them in soil with low fertility in order to determine the effect of this biological agent on germination rate;the growth of seedlings, shoots, and the maturation phase of plants;the rhizosphere’s functional biodiversity;and the resistance of these plants to pathogens. Seeds were soaked in the suspension for a period of two or three hours. During the growing season of the crop, no additional fertilizing and spraying of a biopesticide against diseases or pests occurred. Despite the soil having low fertility, low quantities of organic matter, and not having been before used for the cultivation of agricultural plants, this biofertilizer showed a strong stimulatory effect on the growth of seeds and seedlings of wheat and soybeans. The average germination and seed vigor increased by 1.5 - 2.0 times, and the phenophases were accelerated to three to five days. In all phases of vegetation, the ammonifying bacteria in the presence of an antagonist (a biological agent) developed rapidly and were constantly present in significant numbers in the rhizosphere. Streptomyces fumanus introduced into non-sterile soil entered into competition with the local soil microflora and had the ability to colonize the rhizosphere system of plants. The use of a formulation of Streptomyces gn-2 has improved the composition of rhizosphere microflora, attracting saprophytic microorganisms: ammonificators and oligotrophs. The presence of the biocontrol microorganism Streptomyces fumanus in the rhizosphere plays an important role in enhancing the growth and development of useful groups, such as nitrogen-fixing bacteria.
基金supported by the National Natural Science Foundation of China (41575013)the National Supercomputer Center in Guangzhou, China
文摘Since the early 2000s, many satellite passive microwave brightness temperature （BT） archives, such as the Advanced Microwave Scanning Radiometer for the Earth Observing System （AMSR-E） BTs, have become the useful resources for assessing the changes in the surface and deep soil moistures over both arid and semi-arid regions. In this study, we used a new soil effective temperature （T scheme and the archived AMSR-E BTs to estimate surface soil moisture （SM） over the Nagqu region in the central Tibetan Plateau, China. The surface and deep soil temperatures required for the calculation of regional-scale T were obtained from outputs of the Community Land Model version 4.5 （CLM4.5）. In situ SM measurements at the CEOP-CAMP/Tibet （Coordinated Enhanced Observing Period Asia-Australia Monsoon Project on the Tibetan Plateau） experimental sites were used to validate the AMSR-E-based SM estimations at regional and single-site scales. Furthermore, the spatial distribution of monthly mean surface SM over the Nagqu region was obtained from 16 daytime AMSR-E BT observations in July 2004 over the Nagqu region. Results revealed that the AMSR-E-based surface SM estimations agreed well with the in situ-based surface SM measurements, with the root mean square error （RMSE） ranging from 0.042 to 0.066 m3/m3 and the coefficient of determination （R2） ranging from 0.71 to 0.92 during the nighttime and daytime. The regional surface soil water state map showed a clear spatial pattern related to the terrain. It indicated that the lower surface SM values occurred in the mountainous areas of the northern, mid-western and southeastern parts of Nagqu region, while the higher surface SM values appeared in the low elevation areas such as the Tongtian River Basin, Namco Lake and bog meadows in the central part of Nagqu region. Our analysis also showed that the new T^scheme does not require special fitting parameters or additional assumptions, which simplifies the data requirements for regional-scale applications. This scheme combined with the archived satellite passive microwave BT observations can be used to estimate the historical surface SM for hydrological process studies over the Tibetan Plateau regions.
文摘The negative effect of soil erosion and soil compaction is well documented for the purpose of optimum rangeland functioning, while the impact of rangeland degradation on effective soil depth is seldom quantified. The aim of this study was to quantify the response of vegetation cover and soil properties, particularly effective soil depth and soil texture to rangeland degradation. Forty-one farms were sampled in the arid and semi-arid climate of South Africa. Within these farms, data was collected over a vegetation degradation gradient. Results showed a significant decline in relative basal cover (94% ± 15% to 39% ± 17%) and soil depth (90% ± 14% to 73% ± 24%) as rangeland degraded. Soil texture changes over the degradation gradients vary for different homogeneous vegetation types. Indications regarding the loss of a functioning rangeland ecosystem were also demonstrated, using objective long-term relations between rangeland conditions and grazing capacity. The study highlights the importance of sustainable rangeland management practices to reduce the loss in effective soil depth and to ensure the sustainable utilization of the rangeland ecosystem. These results can probably extrapolate to other arid and semi-arid rangelands worldwide.
文摘A rhizobox system constructed with crude oil- contaminated soil was vegetated with alfalfa （Medicago sativa L.） to evaluate the rhizosphere effects on the soil microbial population and functional structure, and to explore the potential mechanisms by which plants enhance the removal of crude oil in soil. During the 80-day experiment, 31.6% of oil was removed from the adjacent rhizosphere （AR）; this value was 27% and 53% higher than the percentage of oil removed from the far rhizosphere （FR） and from the non-rhizosphere （NR）, respectively. The populations of heterotrophic bacteria and hydrocarbon- degrading bacteria were higher in the AR and FR than in the NR. However, the removal rate of crude oil was positively correlated with the proportion of hydrocarbon- degrading bacteria in the rhizosphere. In total, 796, 731, and 379 functional genes were detected by microarray in the AR, FR, and NR, respectively. Higher proportions of functional genes related to carbon degradation and organic remediation, were found in rhizosphere soil compared with NR soil, suggesting that the rhizosphere selectively increased the abundance of these specific functional genes. The increase in water-holding capacity and decrease in pH as well as salinity of the soil all followed the order of AR 〉 FR 〉 NR. Canonical component analysis showed that salinity was the most important environmental factor influencing the microbial functional structure in the rhizosphere and that salinity was negatively correlated with the abundance of carbon and organic degradation genes.
文摘Heavy tamping is one of the important methods for ground treatment. But the vibration is often a problem that restricts the application during heavy tamping. On the other hand, as an instant impact load with controllable energy, heavy tamping is a good test in situ for the study of soil dynamics. With the analysis in the field of frequency, deductive method for medium effecting function and excitation function of heavy tamping is put forward in this paper.
