A 2,6-dibutoxylnaphthalene-based tetralactam macrocycle was designed and synthesized.This macrocycle shows highly selective recognition to phenazine-a well-known secondary metabolite in bacteria and an emerging disinf...A 2,6-dibutoxylnaphthalene-based tetralactam macrocycle was designed and synthesized.This macrocycle shows highly selective recognition to phenazine-a well-known secondary metabolite in bacteria and an emerging disinfection byproduct in drinking water.In contrast,the macrocycle shows no binding to the structurally similar dibenzo-1,4-dioxin.It was revealed that hydrogen bonding,π-π and σ-π interactions are the major driving forces between phenazine and the new tetralactam macrocycle.A perfect complementarity in electrostatic potential surfaces may explain the high selectivity.In addition,the macrocycle shows fluorescent response to phenazine,demonstrating its potential in fluorescent detection of phenazine.展开更多
The sensing and discrimination of different modified nucleosides are important in various key fields,including disease diagnosis and therapy,vaccine development,and so on.Due to the highly structural similarity and di...The sensing and discrimination of different modified nucleosides are important in various key fields,including disease diagnosis and therapy,vaccine development,and so on.Due to the highly structural similarity and diversity of modified nucleosides,it is urgent but challenging to develop a simple and efficient method for accurate discrimination.Here,the discrimination and multiplex quantitation of modified RNA nucleoside are successfully achieved via a novel tetralactam macrocycle-based fluorescent sensor array for the first time.Based on the excellent ability of the endo-functionalized cavity of tetralactam macrocycle to distinguish structurally similar nucleobases in nucleosides,16 nucleosides(including 4 unmodified,6 methylated,and 6 other chemically modified nucleosides)with similar structures can be simultaneously discriminated with 100%accuracy.Furthermore,the concentrations of individual nucleosides in ternary mixtures are quantified simultaneously by support vector machine regression with high accuracy and immunity to interference,even in complicated human urine.In addition,the accurate discrimination of simulated cancer patient urine is realized by using the 3-element sensor array,validating its great potential in cancer precision medicine.展开更多
基金This research was financially supported by the National Natural Science Foundation of China (Nos. 21705075 and 21822104)the SZSTI (Nos. JCYJ20180504165810828 and KQJSCX 20170728162528382)+2 种基金the Shenzhen Nobel Prize Scientists Laboratory Project (No. C17213101)the Open Fund of State Key Laboratory of Chemo/Biosensing and Chemometrics of Hunan University (No. 2017019)the Academy of Finiand (A.V. grant no. 314343) and the University of Jyvaskyla. We thank SUSTech-MCPC for instrumental assistanee.
文摘A 2,6-dibutoxylnaphthalene-based tetralactam macrocycle was designed and synthesized.This macrocycle shows highly selective recognition to phenazine-a well-known secondary metabolite in bacteria and an emerging disinfection byproduct in drinking water.In contrast,the macrocycle shows no binding to the structurally similar dibenzo-1,4-dioxin.It was revealed that hydrogen bonding,π-π and σ-π interactions are the major driving forces between phenazine and the new tetralactam macrocycle.A perfect complementarity in electrostatic potential surfaces may explain the high selectivity.In addition,the macrocycle shows fluorescent response to phenazine,demonstrating its potential in fluorescent detection of phenazine.
基金supported by the National Natural Science Foundation of China(22174059,22201128)the Young Science and Technology Innovation Program of Hunan Province(2022RC1230).
文摘The sensing and discrimination of different modified nucleosides are important in various key fields,including disease diagnosis and therapy,vaccine development,and so on.Due to the highly structural similarity and diversity of modified nucleosides,it is urgent but challenging to develop a simple and efficient method for accurate discrimination.Here,the discrimination and multiplex quantitation of modified RNA nucleoside are successfully achieved via a novel tetralactam macrocycle-based fluorescent sensor array for the first time.Based on the excellent ability of the endo-functionalized cavity of tetralactam macrocycle to distinguish structurally similar nucleobases in nucleosides,16 nucleosides(including 4 unmodified,6 methylated,and 6 other chemically modified nucleosides)with similar structures can be simultaneously discriminated with 100%accuracy.Furthermore,the concentrations of individual nucleosides in ternary mixtures are quantified simultaneously by support vector machine regression with high accuracy and immunity to interference,even in complicated human urine.In addition,the accurate discrimination of simulated cancer patient urine is realized by using the 3-element sensor array,validating its great potential in cancer precision medicine.
