Objective: to explore the analysis and thinking of the results of two methods for unpaid blood donors in a certain area. Methods: 2000 blood samples from unpaid blood donors in a certain area were selected as the rese...Objective: to explore the analysis and thinking of the results of two methods for unpaid blood donors in a certain area. Methods: 2000 blood samples from unpaid blood donors in a certain area were selected as the research object. The detection method was two times of different manufacturers ELISA reagents (Xinchuang/Wantai/Kehua). Six people were tested (ID-NAT) for those who were negative for both times. If positive, they were split again, subject to the (Roche or Kehua reagents) test (six people). Results: the positive rate of HIV 1/HCV/HBV NAT initial screening was 0.5%. Six samples were positive by ELISA at the same time, and five samples were positive by identification, with the positive rate of identification being 83.33%. The positive blood samples for twice, once positive and once negative were classified as three retest positive samples, and the negative samples for twice were classified as one retest negative sample. The negative initial screening positive S/CO value was 13.75 ± 2.40, and the negative S/CO value was 8.06 ± 6.05;The minimum value of positive S/CO in the initial screening of positive retest is 8.07, the minimum value of negative S/CO is 10.12, the minimum value of positive S/CO in the initial screening of negative retest is 9.13, and the minimum value of negative S/CO is 1.02. Conclusion: the results of ELISA and single ID-NAT test for unpaid blood donors are significant. The two methods can improve the accuracy of blood sample test for unpaid blood donors, and can effectively ensure the safety of clinical blood transfusion treatment.展开更多
文摘Objective: to explore the analysis and thinking of the results of two methods for unpaid blood donors in a certain area. Methods: 2000 blood samples from unpaid blood donors in a certain area were selected as the research object. The detection method was two times of different manufacturers ELISA reagents (Xinchuang/Wantai/Kehua). Six people were tested (ID-NAT) for those who were negative for both times. If positive, they were split again, subject to the (Roche or Kehua reagents) test (six people). Results: the positive rate of HIV 1/HCV/HBV NAT initial screening was 0.5%. Six samples were positive by ELISA at the same time, and five samples were positive by identification, with the positive rate of identification being 83.33%. The positive blood samples for twice, once positive and once negative were classified as three retest positive samples, and the negative samples for twice were classified as one retest negative sample. The negative initial screening positive S/CO value was 13.75 ± 2.40, and the negative S/CO value was 8.06 ± 6.05;The minimum value of positive S/CO in the initial screening of positive retest is 8.07, the minimum value of negative S/CO is 10.12, the minimum value of positive S/CO in the initial screening of negative retest is 9.13, and the minimum value of negative S/CO is 1.02. Conclusion: the results of ELISA and single ID-NAT test for unpaid blood donors are significant. The two methods can improve the accuracy of blood sample test for unpaid blood donors, and can effectively ensure the safety of clinical blood transfusion treatment.
