Cancer comprises a group of diseases which are involved in the aberrant growth of the cells causing disruption of normal body function. Due to the lack of proper sophisticated treatments this nasty disease leads to th...Cancer comprises a group of diseases which are involved in the aberrant growth of the cells causing disruption of normal body function. Due to the lack of proper sophisticated treatments this nasty disease leads to the death of most of the patients affected with it. Moreover, treatments like chemotherapy involve other post-treatment complications which make them unfavorable for extended use. Medicinal plants possess many phytochemicals of great therapeutic value and many of them are effective in killing cancer cells. These compounds working by variety of mechanisms and in most of the cases exhibit their anticancer potentiality by inhibiting many proteins involved in cell growth and division. Molecular docking is a computational approach which facilitates the finding of the best molecule from a group which may bind with the highest affinity with the intended target by providing a virtual biological system. This process works on the basis of specific algorithm and involves scoring function to rank the molecules that fit with the target. This study has been designed to investigate the potentiality of four phytochemicals from Clitoria ternatea—Kaempferol, Myricetin, P-Hydroxycinnamic acid and Quercetin as inhibitors of two cell cycle checkpoint proteins—Cyclin Dependent Kinase-2 (CDK-2) and Cyclin Dependent Kinase-6 (CDK-6) in Cyclin/CDK pathway. Quercetin and Myricetin docked with higher affinity with CDK-2 and CDK-6 respectively. Drug likeness property analysis and ADME/T test impose computational approach to investigate physicochemical and pharmacological properties of candidate drug molecules. P-Hydroxycinnamic acid performed well in both drug likeness property analysis and ADME/T than Quercetin and Myricetin. So, P-Hydroxycinnamic acid is the best finding of this experiment.展开更多
Objective:To assess the estrogenic activity of hydroalcoholic extract of Clitoria(C.)ternatea leaves in female Wistar rats.Methods:Hydroalcoholic extract of C.ternatea leaves prepared by using cold maceration method w...Objective:To assess the estrogenic activity of hydroalcoholic extract of Clitoria(C.)ternatea leaves in female Wistar rats.Methods:Hydroalcoholic extract of C.ternatea leaves prepared by using cold maceration method was tested for estrogenic activity.An acute toxicity study was conducted to estimate the safe dose using OECD 423 guidelines.For estrogenic activity,ovariectomized female rats were divided into four groups,with 6 rats in each group.The control and standard groups were administered with 1%carboxymethyl cellulose orally and estradiol valerate at 1μg/rat/day subcutaneously,respectively.The third group was administered with hydroalcoholic extract of C.ternatea at the dose 500 mg/kg body weight orally and the fourth group was administered with hydroalcoholic extract of C.ternatea at the dose 500 mg/kg body weight orally along with estradiol valerate at dose 1μg/rat/day subcutaneously.All treatments lasted for 7 consecutive days and estrogenic activity was assessed by observing vaginal cornfication.On day 8,all animals were sacrificed and uterine horns were dissected out.Utrine weight was measured and blood serum was further processed for the estimation of biochemical parameters like cholesterol,total proteins,alkaline phosphatase and estrogen by autoanylser.Histological studies of uterus were also carried out.Results:Acute toxicity studies indicated the hydroalcoholic extract of C.ternatea leave was found to be safe up to the dose level of 2000 mg/kg.Oral administration of C.ternatea extract at the dose 500 mg/kg body weight and and estradiol valerate(1μg/rat/day)caused morphological changes i.e.increase in uterine weight,vaginal opening and cornification of cells;biochemical changes i.e.increase in cholesterol,total protein,alkaline phosphatase and estrogen contents;histological changes i.e.increase in uterine diameter,thickness and height of endometrium.Simultaneous administration of C.ternatea extract with estradiol valerate showed a synergistic effect.Histological investigations further confirmed the strong estrogenic nature of C.ternatea extract.Conclusions:C.ternatea extract(500 mg/kg)showed a significant estrogenic activity which is also supported by biochemical and histological studies.So,on the basis of these findings,it can be concluded that C.ternatea can be used as an alternative to synthetic oral contraceptives.展开更多
Ayurveda is a renowned traditional medicine practiced in India from ancient times and Clitoria ternatea is one such prospective medicinal herb incorporated as an essential constituent in a brain tonic called as medhya...Ayurveda is a renowned traditional medicine practiced in India from ancient times and Clitoria ternatea is one such prospective medicinal herb incorporated as an essential constituent in a brain tonic called as medhya rasayan for treating neurological disorders.This work emphasises the significance of the plant as a brain drug there by upholding Indian medicine.The phytochemicals from the root extract were extricated using gas chromatography–mass spectrometry assay and molecular docking against the protein Monoamine oxidase was performed with four potential compounds along with four reference compounds of the plant.This persuades the prospect of C.ternatea as a remedy for neurodegenerative diseases and depression.The in silico assay enumerates that a major compound(Z)-9,17-octadecadienal obtained from the chromatogram with a elevated retention time of 32.99 furnished a minimum binding affinity energy value of-6.5 kcal/mol against monoamine oxidase(MAO-A).The interactions with the amino acid residues ALA 68,TYR 60 and TYR 69 were analogous to the reference compound kaempferol-3-monoglucoside with a least score of-13.90/-12.95 kcal/mol against the isoforms(MAO)A and B.This study fortifies the phytocompounds of C.ternatea as MAO-inhibitors and to acquire a pharmaceutical approach in rejuvenating Ayurvedic medicine.展开更多
Clitoria ternatea L.,belonging to the family Fabaceae and subfamily Papilonaceae,is an herbaceous perennial legume valued for its medicinal importance.Pharmacological investigations on this plant have shown it has a v...Clitoria ternatea L.,belonging to the family Fabaceae and subfamily Papilonaceae,is an herbaceous perennial legume valued for its medicinal importance.Pharmacological investigations on this plant have shown it has a variety of medicinal properties,including antibacterial activities,antitumor activities,antioxidant activities,memory enhancing activities,wound healing activities,antipyretic activities and antiasthmatic activities.However,there are few studies on the chemical constituents of C.ternatea.The present research reviewed the chemical constituents and biological activities of C.ternatea to provide references for further research on this plant.展开更多
Objective:Clitoria ternatea is a well-known bioactive plant used to treat several inflammatory ailments in Ayurvedic system of medicine in India.The present investigation aimed to determine the antiinflammatory and an...Objective:Clitoria ternatea is a well-known bioactive plant used to treat several inflammatory ailments in Ayurvedic system of medicine in India.The present investigation aimed to determine the antiinflammatory and anti-arthritic activity of ethanolic extract of Clitoria ternatea roots(EECT) in animal models.Methods:The anti-inflammatory activity of the EECT was evaluated by carrageenan and histamineinduced paw edema.Results:EECT showed a significant reduction in mean paw edema volume in both carrageenan and histamine-induced inflammation.The efficacy of EECT in rheumatoid arthritis was tested against Freund's complete adjuvant(CFA) induced arthritic model in Wistar rats.The anti-arthritic effect of EECT was determined by systematic scoring of arthritis symptoms and measuring paw edema.A considerable decrease in paw diameter was observed in the EECT(200 and 400 mg/kg) and diclofenac(10 mg/kg) treated groups after day 7.Diclofenac(10 mg/kg) and EECT(400 mg/kg) showed a significant reduction in paw diameter from day 14 compared with CFA control(P <0.001).The anti-arthritic activity was also confirmed from the altered biochemical,haematological(Hb,RBC and WBC) and anti-oxidant parameters(SOD,MDA,CAT,and GSH).EECT(400 and 200 mg/kg) also showed a marked inhibition of joint destruction.Conclusion:This study provides a pharmacological rationale for the traditional use of C.ternatea against inflammation and rheumatoid arthritis in India.展开更多
文摘Cancer comprises a group of diseases which are involved in the aberrant growth of the cells causing disruption of normal body function. Due to the lack of proper sophisticated treatments this nasty disease leads to the death of most of the patients affected with it. Moreover, treatments like chemotherapy involve other post-treatment complications which make them unfavorable for extended use. Medicinal plants possess many phytochemicals of great therapeutic value and many of them are effective in killing cancer cells. These compounds working by variety of mechanisms and in most of the cases exhibit their anticancer potentiality by inhibiting many proteins involved in cell growth and division. Molecular docking is a computational approach which facilitates the finding of the best molecule from a group which may bind with the highest affinity with the intended target by providing a virtual biological system. This process works on the basis of specific algorithm and involves scoring function to rank the molecules that fit with the target. This study has been designed to investigate the potentiality of four phytochemicals from Clitoria ternatea—Kaempferol, Myricetin, P-Hydroxycinnamic acid and Quercetin as inhibitors of two cell cycle checkpoint proteins—Cyclin Dependent Kinase-2 (CDK-2) and Cyclin Dependent Kinase-6 (CDK-6) in Cyclin/CDK pathway. Quercetin and Myricetin docked with higher affinity with CDK-2 and CDK-6 respectively. Drug likeness property analysis and ADME/T test impose computational approach to investigate physicochemical and pharmacological properties of candidate drug molecules. P-Hydroxycinnamic acid performed well in both drug likeness property analysis and ADME/T than Quercetin and Myricetin. So, P-Hydroxycinnamic acid is the best finding of this experiment.
文摘Objective:To assess the estrogenic activity of hydroalcoholic extract of Clitoria(C.)ternatea leaves in female Wistar rats.Methods:Hydroalcoholic extract of C.ternatea leaves prepared by using cold maceration method was tested for estrogenic activity.An acute toxicity study was conducted to estimate the safe dose using OECD 423 guidelines.For estrogenic activity,ovariectomized female rats were divided into four groups,with 6 rats in each group.The control and standard groups were administered with 1%carboxymethyl cellulose orally and estradiol valerate at 1μg/rat/day subcutaneously,respectively.The third group was administered with hydroalcoholic extract of C.ternatea at the dose 500 mg/kg body weight orally and the fourth group was administered with hydroalcoholic extract of C.ternatea at the dose 500 mg/kg body weight orally along with estradiol valerate at dose 1μg/rat/day subcutaneously.All treatments lasted for 7 consecutive days and estrogenic activity was assessed by observing vaginal cornfication.On day 8,all animals were sacrificed and uterine horns were dissected out.Utrine weight was measured and blood serum was further processed for the estimation of biochemical parameters like cholesterol,total proteins,alkaline phosphatase and estrogen by autoanylser.Histological studies of uterus were also carried out.Results:Acute toxicity studies indicated the hydroalcoholic extract of C.ternatea leave was found to be safe up to the dose level of 2000 mg/kg.Oral administration of C.ternatea extract at the dose 500 mg/kg body weight and and estradiol valerate(1μg/rat/day)caused morphological changes i.e.increase in uterine weight,vaginal opening and cornification of cells;biochemical changes i.e.increase in cholesterol,total protein,alkaline phosphatase and estrogen contents;histological changes i.e.increase in uterine diameter,thickness and height of endometrium.Simultaneous administration of C.ternatea extract with estradiol valerate showed a synergistic effect.Histological investigations further confirmed the strong estrogenic nature of C.ternatea extract.Conclusions:C.ternatea extract(500 mg/kg)showed a significant estrogenic activity which is also supported by biochemical and histological studies.So,on the basis of these findings,it can be concluded that C.ternatea can be used as an alternative to synthetic oral contraceptives.
文摘Ayurveda is a renowned traditional medicine practiced in India from ancient times and Clitoria ternatea is one such prospective medicinal herb incorporated as an essential constituent in a brain tonic called as medhya rasayan for treating neurological disorders.This work emphasises the significance of the plant as a brain drug there by upholding Indian medicine.The phytochemicals from the root extract were extricated using gas chromatography–mass spectrometry assay and molecular docking against the protein Monoamine oxidase was performed with four potential compounds along with four reference compounds of the plant.This persuades the prospect of C.ternatea as a remedy for neurodegenerative diseases and depression.The in silico assay enumerates that a major compound(Z)-9,17-octadecadienal obtained from the chromatogram with a elevated retention time of 32.99 furnished a minimum binding affinity energy value of-6.5 kcal/mol against monoamine oxidase(MAO-A).The interactions with the amino acid residues ALA 68,TYR 60 and TYR 69 were analogous to the reference compound kaempferol-3-monoglucoside with a least score of-13.90/-12.95 kcal/mol against the isoforms(MAO)A and B.This study fortifies the phytocompounds of C.ternatea as MAO-inhibitors and to acquire a pharmaceutical approach in rejuvenating Ayurvedic medicine.
文摘Clitoria ternatea L.,belonging to the family Fabaceae and subfamily Papilonaceae,is an herbaceous perennial legume valued for its medicinal importance.Pharmacological investigations on this plant have shown it has a variety of medicinal properties,including antibacterial activities,antitumor activities,antioxidant activities,memory enhancing activities,wound healing activities,antipyretic activities and antiasthmatic activities.However,there are few studies on the chemical constituents of C.ternatea.The present research reviewed the chemical constituents and biological activities of C.ternatea to provide references for further research on this plant.
文摘Objective:Clitoria ternatea is a well-known bioactive plant used to treat several inflammatory ailments in Ayurvedic system of medicine in India.The present investigation aimed to determine the antiinflammatory and anti-arthritic activity of ethanolic extract of Clitoria ternatea roots(EECT) in animal models.Methods:The anti-inflammatory activity of the EECT was evaluated by carrageenan and histamineinduced paw edema.Results:EECT showed a significant reduction in mean paw edema volume in both carrageenan and histamine-induced inflammation.The efficacy of EECT in rheumatoid arthritis was tested against Freund's complete adjuvant(CFA) induced arthritic model in Wistar rats.The anti-arthritic effect of EECT was determined by systematic scoring of arthritis symptoms and measuring paw edema.A considerable decrease in paw diameter was observed in the EECT(200 and 400 mg/kg) and diclofenac(10 mg/kg) treated groups after day 7.Diclofenac(10 mg/kg) and EECT(400 mg/kg) showed a significant reduction in paw diameter from day 14 compared with CFA control(P <0.001).The anti-arthritic activity was also confirmed from the altered biochemical,haematological(Hb,RBC and WBC) and anti-oxidant parameters(SOD,MDA,CAT,and GSH).EECT(400 and 200 mg/kg) also showed a marked inhibition of joint destruction.Conclusion:This study provides a pharmacological rationale for the traditional use of C.ternatea against inflammation and rheumatoid arthritis in India.
