In order to study the functional structure of the transcription terminators and the mechanism of termination, a survey of the chromatin structure, including the location of DNase I hypersensitive sites and the nucleos...In order to study the functional structure of the transcription terminators and the mechanism of termination, a survey of the chromatin structure, including the location of DNase I hypersensitive sites and the nucleosome arrangement, of yepst ADH1 and FLP terminators was made. The results show that there is no relationship between the function of the terminators and the existence of DNase I hypersensitive sites. However, it is found that there is always a nueleosome at the immediate upstream of the transcrip-tional termination sites. As a control, the chromatin structures of the pBR322 DNA fragments on the yeast shutter vectors are also investigated at the same time. The random nucleosome arrangement on the bacterial DNA in yeast agrees with the published reports. A new hypothesis, about the mechanism of transcriptional termination is put forward and the reason of different nucleosome arrangement on the DNAs which areori-ginally from different species in yeast is discussed.展开更多
文摘In order to study the functional structure of the transcription terminators and the mechanism of termination, a survey of the chromatin structure, including the location of DNase I hypersensitive sites and the nucleosome arrangement, of yepst ADH1 and FLP terminators was made. The results show that there is no relationship between the function of the terminators and the existence of DNase I hypersensitive sites. However, it is found that there is always a nueleosome at the immediate upstream of the transcrip-tional termination sites. As a control, the chromatin structures of the pBR322 DNA fragments on the yeast shutter vectors are also investigated at the same time. The random nucleosome arrangement on the bacterial DNA in yeast agrees with the published reports. A new hypothesis, about the mechanism of transcriptional termination is put forward and the reason of different nucleosome arrangement on the DNAs which areori-ginally from different species in yeast is discussed.
