The nucleus accumbens(NAc)plays an important role in various emotional and motivational behaviors that rely on heightened wakefulness.However,the neural mechanisms underlying the relationship between arousal and emoti...The nucleus accumbens(NAc)plays an important role in various emotional and motivational behaviors that rely on heightened wakefulness.However,the neural mechanisms underlying the relationship between arousal and emotion regulation in NAc remain unclear.Here,we investigated the roles of a specific subset of inhibitory corticotropin-releasing hormone neurons in the NAc(NAcCRH)in regulating arousal and emotional behaviors in mice.We found an increased activity of NAcCRH neurons during wakefulness and rewarding stimulation.Activation of NAcCRH neurons converts NREM or REM sleep to wakefulness,while inhibition of these neurons attenuates wakefulness.Remarkably,activation of NAcCRH neurons induces a place preference response(PPR)and decreased basal anxiety level,whereas their inactivation induces a place aversion response and anxious state.NAcCRH neurons are identified as the major NAc projection neurons to the bed nucleus of the stria terminalis(BNST).Furthermore,activation of the NAcCRH-BNST pathway similarly induced wakefulness and positive emotional behaviors.Taken together,we identified a basal forebrain CRH pathway that promotes the arousal associated with positive affective states.展开更多
The insulin-like growth factor Ⅰ(IGF-Ⅰ) gene of triangular bream (Megalobrama terminalis)(GenBank No.AY247412)(Tb)was cloned for the first time from liver by RT-PCR. Thenucleotide sequence analysis showed the Tb IGF...The insulin-like growth factor Ⅰ(IGF-Ⅰ) gene of triangular bream (Megalobrama terminalis)(GenBank No.AY247412)(Tb)was cloned for the first time from liver by RT-PCR. Thenucleotide sequence analysis showed the Tb IGF-ⅠcDNA consisted of 486 nucleotides andencoded 117 amino acids including B, C, A, D and E five domains. Analysis of E-domainindicated that cloned Tb IGF-Ⅰbelonged to IGF-ⅠEa-2 subtype. Identity analysis showedthe IGF-Ⅰnucleotide sequence shared 99.8% homology with bluntnose bream, 88.8% withgrass carp, 85.8% with common carp; the pre-IGF-Ⅰamine acid sequence shared 99.4% withbluntnose bream, 88.8% with grass carp, 85.4% homology with common carp. In the CyprinusCarpio, the higher homology of nucleotide sequence and amino acid sequence in IGF-Ⅰshowed that the closer relationship the fishes have. These results could provide basicdata for the research on Tb germplasm and the development and utilization of biologicalfeed additives.展开更多
A 62-year-old woman with frequent occurrence of symptomatic atrial tachycardia with a foci located at the root of the upper crista terminalis was found to have right diaphragm paresis after receiving a total of 8 radi...A 62-year-old woman with frequent occurrence of symptomatic atrial tachycardia with a foci located at the root of the upper crista terminalis was found to have right diaphragm paresis after receiving a total of 8 radiofrequency energy deliveries （40-60 W, 50-60℃） and a total duration of 540 seconds of ablation therapy （7Fr 8 mm deflectable ablation catheter）. The right diaphragm paresis remained resolved up to 14 months after the procedure as confirmed by repeated chest X-rays.展开更多
Anxiety is a common disease in the modern society which significantly affects people’s daily lives and function,thus it has become an increasingly highlighted issue.Anxiety is regulated by neural circuits in the brai...Anxiety is a common disease in the modern society which significantly affects people’s daily lives and function,thus it has become an increasingly highlighted issue.Anxiety is regulated by neural circuits in the brain.Therefore,the basal mechanism of anxiety has been studied,especially research based on the related neural circuits.For a long time,due to the limitations of science and technology,there was no breakthrough in research regarding anxiety.However,in recent years,due to the progress of technology,the research on anxiety neural circuits has made great progress.For example,the interaction among various brain regions,such as the central nucleus of the amygdala(CeA),the ventral tegmental area(VTA),the ventral hippocampus(vHPC),and so on.This article focuses on three brain regions:including BLA,BNST,and VTA,and illustrate their different roles and mechanisms in regulating anxiety.On this basis,this intensive study of anxiety will further promote the progress of anxiety research and provide therapeutic targets for the related treatment.展开更多
基金supported by grants from the National Natural Science Foundation of China(82090033,82288101,U20A6005,32200801,and 32200967)China Postdoctoral Science Foundation(2021M700851,2023T160117,and 2023M740708).
文摘The nucleus accumbens(NAc)plays an important role in various emotional and motivational behaviors that rely on heightened wakefulness.However,the neural mechanisms underlying the relationship between arousal and emotion regulation in NAc remain unclear.Here,we investigated the roles of a specific subset of inhibitory corticotropin-releasing hormone neurons in the NAc(NAcCRH)in regulating arousal and emotional behaviors in mice.We found an increased activity of NAcCRH neurons during wakefulness and rewarding stimulation.Activation of NAcCRH neurons converts NREM or REM sleep to wakefulness,while inhibition of these neurons attenuates wakefulness.Remarkably,activation of NAcCRH neurons induces a place preference response(PPR)and decreased basal anxiety level,whereas their inactivation induces a place aversion response and anxious state.NAcCRH neurons are identified as the major NAc projection neurons to the bed nucleus of the stria terminalis(BNST).Furthermore,activation of the NAcCRH-BNST pathway similarly induced wakefulness and positive emotional behaviors.Taken together,we identified a basal forebrain CRH pathway that promotes the arousal associated with positive affective states.
文摘The insulin-like growth factor Ⅰ(IGF-Ⅰ) gene of triangular bream (Megalobrama terminalis)(GenBank No.AY247412)(Tb)was cloned for the first time from liver by RT-PCR. Thenucleotide sequence analysis showed the Tb IGF-ⅠcDNA consisted of 486 nucleotides andencoded 117 amino acids including B, C, A, D and E five domains. Analysis of E-domainindicated that cloned Tb IGF-Ⅰbelonged to IGF-ⅠEa-2 subtype. Identity analysis showedthe IGF-Ⅰnucleotide sequence shared 99.8% homology with bluntnose bream, 88.8% withgrass carp, 85.8% with common carp; the pre-IGF-Ⅰamine acid sequence shared 99.4% withbluntnose bream, 88.8% with grass carp, 85.4% homology with common carp. In the CyprinusCarpio, the higher homology of nucleotide sequence and amino acid sequence in IGF-Ⅰshowed that the closer relationship the fishes have. These results could provide basicdata for the research on Tb germplasm and the development and utilization of biologicalfeed additives.
文摘A 62-year-old woman with frequent occurrence of symptomatic atrial tachycardia with a foci located at the root of the upper crista terminalis was found to have right diaphragm paresis after receiving a total of 8 radiofrequency energy deliveries （40-60 W, 50-60℃） and a total duration of 540 seconds of ablation therapy （7Fr 8 mm deflectable ablation catheter）. The right diaphragm paresis remained resolved up to 14 months after the procedure as confirmed by repeated chest X-rays.
文摘Anxiety is a common disease in the modern society which significantly affects people’s daily lives and function,thus it has become an increasingly highlighted issue.Anxiety is regulated by neural circuits in the brain.Therefore,the basal mechanism of anxiety has been studied,especially research based on the related neural circuits.For a long time,due to the limitations of science and technology,there was no breakthrough in research regarding anxiety.However,in recent years,due to the progress of technology,the research on anxiety neural circuits has made great progress.For example,the interaction among various brain regions,such as the central nucleus of the amygdala(CeA),the ventral tegmental area(VTA),the ventral hippocampus(vHPC),and so on.This article focuses on three brain regions:including BLA,BNST,and VTA,and illustrate their different roles and mechanisms in regulating anxiety.On this basis,this intensive study of anxiety will further promote the progress of anxiety research and provide therapeutic targets for the related treatment.
