Temperature-sensitive hydrogel—poly(N-isopropyl acrylamide) (PNIPA) was prepared and applied to protein refolding. PNIPA gel disks and gel particles were synthesized by the solution polymerization and inverse suspens...Temperature-sensitive hydrogel—poly(N-isopropyl acrylamide) (PNIPA) was prepared and applied to protein refolding. PNIPA gel disks and gel particles were synthesized by the solution polymerization and inverse suspension polymerization respectively. The swelling kinetics of the gels was also studied. With these prepared PNIPA gels, the model protein lysozyme was renatured. Within 24h, PNIPA gel disks improved the yield of lysozyme activity by 49.3% from 3375.2U·mg^-1 to 5038.8U·mg^-1. With the addition of faster response PNIPA gel beads, the total lysozyme activity recovery was about 68.98% in 3h, as compared with 42.03% by simple batch dilution. The novel refolding system with PNIPA enables efficient refolding especially at high protein concentrations. Discussion about the mechanism revealed that when PNIPA gels were added into the refolding buffer, the hydrophobic interactions between denatured proteins and polymer gels could prevent the aggregation of refolding intermediates, thus enhanced the protein renaturation.展开更多
In this study,the hydrogels composites with coatings based on a temperature-sensitive linear copolymer of N-tert-butylacrylamide(NTBA)and acrylamide(AAm)on cotton fabrics have been developed.The cotton fabrics were co...In this study,the hydrogels composites with coatings based on a temperature-sensitive linear copolymer of N-tert-butylacrylamide(NTBA)and acrylamide(AAm)on cotton fabrics have been developed.The cotton fabrics were coated using aqueous solution of the linear copolymer,1,2,3,4-butanetertracarboxylic acid(BTCA)as a cross-linker and sodium hypophosphite(SHP)as a catalyst,followed by drying and curing.The effects of cross-linking reaction conditions in coating process on water-impermeable ability of coated cotton fabrics were investigated in detail.The results indicate that the coated fabrics have temperature sensitivity.The coatings of poly(NTBA-co-AAm)hydrogels were bonded on the surface of the cotton fabrics,as verified by SEM and optical microscopy,which gave the water-impermeable ability to the hydrogels composites.Moreover,the hydrogels formed in the coating process also identified that - COOH of BTCA reacted with -NH2 in the linear polymer and formed three-dimensional network hydrogels.FTIR and XPS were used to characterize the cross-linking reaction of - COOH of BTCA and - OH of cellulose.展开更多
The swelling behavior of a temperature-sensitive poly-N-isopropylacrylamide(PNIPAM) hydrogel circular cylinder is studied subjected to combined extension-torsion and varied temperature. In this regard, a semi-analytic...The swelling behavior of a temperature-sensitive poly-N-isopropylacrylamide(PNIPAM) hydrogel circular cylinder is studied subjected to combined extension-torsion and varied temperature. In this regard, a semi-analytical solution is proposed for general combined loading. A finite element(FE) analysis is conducted, subjecting a hydrogel cylinder to the combined extension-torsion and the varied temperature to evaluate the validity and accuracy of the solution. A user-defined UHYPER subroutine is developed and verified under free and constrained swelling conditions. The FE results illustrate excellent agreement with the semi-analytical solution. Due to the complexity of the problem, some compositions and applied loading factors are analyzed. It is revealed that for larger cross-linked density and larger ending temperature, the cylinder yields higher stresses and smaller radial swelling deformation. Besides, the radial and hoop stresses increase by applying larger twist and axial stretch. The hoop stresses intersect at approximately R/Rout = 0.58, where the hoop stress vanishes. Besides, the axial force has direct and inverse relationships with the axial stretch and the twist, respectively. However, the resultant torsional moment behavior is complex, and the position of the maximum point varies significantly by altering the axial stretch and the twist.展开更多
A kind of novel copolymer hydrogel of poly（N, N-dimethylaminoethyl methacrylate-co-N-isopropylacrylamide） （poly[DMAEMA/NIPAAm]） was synthesized by the initiation of K2S2O8, N, N＇-methylene-bis（acrylamide） （Bis...A kind of novel copolymer hydrogel of poly（N, N-dimethylaminoethyl methacrylate-co-N-isopropylacrylamide） （poly[DMAEMA/NIPAAm]） was synthesized by the initiation of K2S2O8, N, N＇-methylene-bis（acrylamide） （Bis） was used as the crosslinker. The effects of monomer content, pH and temperature on swelling ratio of the hydrogel were investigated; the thermo-sensitivity in deionized water and in physiological saline was determined. It showed that the swelling ratio of the hydrogel could be changed by changing the temperature or pH alternately. Both swelling ratio and LCST （Lower Critical Solution Temperature） of the hydrogel decreased with the increase of NIPAAm in the co-polymer content.展开更多
Nanoscale zero-valent iron(n ZVI) particles supported on a porous, semi-interpenetrating(semi-IPN), temperature-sensitive composite hydrogel(PNIPAm-PHEMA). n ZVI@PNIPAmPHEMA, was successfully synthesized and character...Nanoscale zero-valent iron(n ZVI) particles supported on a porous, semi-interpenetrating(semi-IPN), temperature-sensitive composite hydrogel(PNIPAm-PHEMA). n ZVI@PNIPAmPHEMA, was successfully synthesized and characterized by FT-IR, SEM, EDS, XRD and the weighing method. The loading of nZVI was 0.1548 ± 0.0015 g/g and the particle size was30–100 nm. NZVI was uniformly dispersed on the pore walls inside the PNIPAm-PHEMA.Because of the well-dispersed n ZVI, the highly porous structure, and the synergistic effect of PNIPAm-PHEMA, nZVI@PNIPAm-PHEMA showed excellent reductive activity and wide p H applicability. 95% of 4-NP in 100 m L of 400 mg/L 4-NP solution with initial p H 3.0–9.0 could be completely reduced into 4-AP by about 0.0548 g of fresh supported n ZVI at 18–25 °C under stirring(110 r/min) within 45 min reaction time. A greater than 99% 4-NP degradation ratio was obtained when the initial p H was 5.0–9.0. The reduction of 4-NP by nZVI@PNIPAm-PHEMA was in agreement with the pseudo-first-order kinetics model with Kobsvalues of 0.0885–0.101 min-1.NZVI@PNIPAm-PHEMA was able to be recycled, and about 85% degradation ratio of 4-NP was obtained after its sixth reuse cycle. According to the temperature sensitivity of PNIPAmPHEMA, n ZVI@PNIPAm-PHEMA exhibited very good storage stability, and about 88.9%degradation ratio of 4-NP was obtained after its storage for 30 days. The hybrid reducer was highly efficient for the reduction of 2-NP, 3-NP, 2-chloro-4-nitrophenol and 2-chloro-4-nitrophenol. Our results suggest that PNIPAm-PHEMA could be a good potential carrier, with n ZVI@PNIPAm-PHEMA having potential value in the application of reductive degradation of nitrophenol pollutants.展开更多
Atherosclerosis remains a major cause of morbidity and mortality worldwide.Intraplaque neovascularization critically promotes atherosclerotic progression and instability.Vascular endothelial growth factor A(VEGFA)stim...Atherosclerosis remains a major cause of morbidity and mortality worldwide.Intraplaque neovascularization critically promotes atherosclerotic progression and instability.Vascular endothelial growth factor A(VEGFA)stimulates aberrant microvessel growth in plaques by inducing endothelial cell proliferation and migration.Pigment epithelium-derived factor(PEDF)potently inhibits VEGFA-dependent neovascularization.This study introduces a thermosensitive hydrogel(PFSgel)developed from poloxamer 407(F127)and sodium alginate(SA)to deliver PEDF locally to atherosclerotic lesions.The PFSgel demonstrated a suitable liquid-solid transition at body temperature(37°C),then forming a stable 3D network structure after SA gelling with the Ca2+in the physiological environment which contributed to the character of controlled release.Rheological analysis confirmed its phase transition temperature of 28.7°C and notable self-healing properties,making it ideal for dynamic vascular environments.In vitro experiments showed that PFSgel could suppress VEGFA-induced endothelial cells’proliferation and migration through modulation of CD31 and MMP-2/MMP-9 signaling.Notably,in vivo degradation test validated the controlled release pattern of PFSgel.In Apoedeficient atherosclerotic mice,ultrasound-guided PFSgel injection onto the abdominal aorta enabled gradual in situ release of encapsulated PEDF.This effectively reduced plaque burden,neovascularization,and luminal stenosis,even with exogenous VEGFA administration.Histological analyses confirmed reduced lipid deposition,plaque area,and neovascularization within plaques.Overall,this novel in situforming PEDF delivery platform enables targeted suppression of pathological neovascularization via CD31 and MMP-2/MMP-9 pathways,representing a promising approach to stabilize high-risk plaques by intervening against VEGFA-dependent neovascularization.展开更多
Flexible hydrogels have shown promise as strain sensors in medical monitoring,human motion detection and intelligent robotics.For a hydrogel strain sensor,certain challenges need to be urgently addressed for practical...Flexible hydrogels have shown promise as strain sensors in medical monitoring,human motion detection and intelligent robotics.For a hydrogel strain sensor,certain challenges need to be urgently addressed for practical applications,such as the damage caused by external effects,leading to equipment failure,and the inability to perceive ambient temperature,resulting in single functionality.Herein,a stretchable,self-healing and dual temperature-strain sensitive hydrogel,with a physically-crosslinked network,is designed by constructing multiple dynamic reversible bonds.Graphene oxide(GO)and iron ions(Fe^(3+))act as dynamic bridges in the cross-linked network and are mediated by the covalent and hydrogen bonding,rendering excellent stretchability to the hydrogel.The reversible features of coordination interactions and hydrogen interactions endow excellent recoverability and self-healing properties.Moreover,the incorporated N-isopropyl acrylamide(NIPAM)provides excellent temperature responsiveness to the hydrogel,facilitating the detection of external temperature changes.Meanwhile,the hydrogels exhibited strain-sensitivity,with a wide working range of 1%-300%,fast response and electrical stability,which can be used as flexible sensors to monitor body motions,e.g.,speaking and the bending of finger,wrist,elbow and knee.Overall,the hydrogel possesses dual sensory capabilities,combining external temperature and strain,for potential applications in wearable multifunctional sensing devices.展开更多
Post-traumatic stress disorder(PTSD)is a psychiatric disease that seriously affects brain function.Currently,selective serotonin reuptake inhibitors(SSRIs)are used to treat PTSD clinically but have decreased efficienc...Post-traumatic stress disorder(PTSD)is a psychiatric disease that seriously affects brain function.Currently,selective serotonin reuptake inhibitors(SSRIs)are used to treat PTSD clinically but have decreased efficiency and increased side effects.In this study,nasal cannabidiol inclusion complex temperature-sensitive hydrogels(CBD TSGs)were prepared and evaluated to treat PTSD.Mice model of PTSD was established with conditional fear box.CBD TSGs could significantly improve the spontaneous behavior,exploratory spirit and alleviate tension in open field box,relieve anxiety and tension in elevated plus maze,and reduce the freezing time.Hematoxylin and eosin and c-FOS immunohistochemistry slides showed that the main injured brain areas in PTSD were the prefrontal cortex,amygdala,and hippocampus CA1.CBD TSGs could reduce the level of tumor necrosis factor-a caused by PTSD.Western blot analysis showed that CBD TSGs increased the expression of the 5-HT1 A receptor.Intranasal administration of CBD TSGs was more efficient and had more obvious brain targeting effects than oral administration,as evidenced by the pharmacokinetics and brain tissue distribution of CBD TSGs.Overall,nasal CBD TSGs are safe and effective and have controlled release.There are a novel promising option for the clinical treatment of PTSD.展开更多
Semi-interpenetrating （semi-IPNs） hydrogels containing biocompatible silk sericin （SS） and poly（N-isopropylacrylamide）（PNIPAM） were prepared as novel cellular matrices. Their maximum swelling degree and basic ...Semi-interpenetrating （semi-IPNs） hydrogels containing biocompatible silk sericin （SS） and poly（N-isopropylacrylamide）（PNIPAM） were prepared as novel cellular matrices. Their maximum swelling degree and basic characteristics for biomedical applications such as mouse ？broblasts （L929） cell proliferation and desorption were investigated. The results showed that the incorporation of high hydrophilic SS into PNIPAM hydrogel increased the maximum swelling degree of the semi-IPNs hydrogels, and the adhesion and growth of the L929 on semi-IPNs hydrogels were at least comparable to, or even better than, that on conventional PNIPAM hydrogel. In addition, L929 cells were found to detach from the hydrogels surface naturally by controlling environmental temperature. These results suggest great potential of semi-IPNs hydrogels in tissue engineering.展开更多
Insulin is an essential and versatile protein taking part in the control of blood glucose levels and protein anabolism.However,under prolonged storage or high temperature stress,insulin tends to unfold and aggregate i...Insulin is an essential and versatile protein taking part in the control of blood glucose levels and protein anabolism.However,under prolonged storage or high temperature stress,insulin tends to unfold and aggregate into toxic amyloid fibrils,leading to loss of physiological function.Inspired by natural chaperones,a series of temperature-sensitive polycaprolactone-based micelles were designed to prevent insulin from deactivation.The micelles were fabricated through the self-assembly of amphiphilic copolymers of methoxy poly(ethylene glycol)-poly(4-diethylformamide caprolactone-co-caprolactone)(mPEG_(17)-P(DECL-co-CL)),which had a regular spherical morphology with particle sizes of about 100 nm.In addition,the lower critical solution temperature(LCST)of the micelles could be tuned to 9 and 29℃by changing the ratio of DECL to CL.Benefiting from the temperature-sensitivity of DECL segment,the binding ability of micelles to insulin could be modulated by changing the temperature.Above LCST,micelles effectively inhibited insulin aggregation and protected it from thermal inactivation due to the strong binding ability between the hydrophobic segment DECL and insulin.Below LCST,DECL segment returned to hydrophilic and bound weakly with insulin,leading to the release of insulin and assisting in its recovery of secondary structure.Thus,these temperature-sensitive micelles provided an effective strategy for insulin protection.展开更多
A temperature-sensitive mud cake remover(G315)was developed using ethyl lactate as the primary component.Based on this,a temperature-sensitive acidic completion fluid(CF-G315)was formulated.Core evaluation tests,mud c...A temperature-sensitive mud cake remover(G315)was developed using ethyl lactate as the primary component.Based on this,a temperature-sensitive acidic completion fluid(CF-G315)was formulated.Core evaluation tests,mud cake dissolution tests and corrosion tests were conducted to analyze the mud cake removal performance of G315,the removal efficiency of CF-G315,and its ability to modify the near-wellbore reservoirs,corrosion to casing and hydrolysis performance.Results indicate that ethyl lactate in G315 exhibits weak acidity at room temperature and decomposes into lactic acid under high temperatures.The lactic acid reacts with the calcium carbonate in the mud cake,generating bubbles that dislodge the mud cake and form soluble salts that are subsequently removed by fluid flow,thereby ensuring effective mud cake clearance.CF-G315 removes mud cake efficiently and enhances near-wellbore reservoir permeability.It demonstrates low corrosivity and environmental compatibility,contributing to equipment safety,simplified operational procedures and reduced operational risks.CF-G315 is promising for application in scenarios such as horizontal wells,open-hole completions and gravel pack completions.展开更多
To get a sort of new scaffold material for soft tissue reconstruction,we have prepared XLHA-PNIPAAm and XLHA-MC injectable hydrogels through blending crosslinked HA(XLHA) and two temperature-sensitive materials differ...To get a sort of new scaffold material for soft tissue reconstruction,we have prepared XLHA-PNIPAAm and XLHA-MC injectable hydrogels through blending crosslinked HA(XLHA) and two temperature-sensitive materials differed in degradation poly(N-isopropylacrylamide)(PNIPAAm) and methylcellulose(MC),respectively.We tested the injectablility,enzymatic biodegradability,temperature-sensitivity,structure cytotoxicity and hemolysis of the two injectable hydrogels.Our research has successfully obtained the preparation condition of XLHA-PNIPAAm injectable hydrogel,and verified that adding non-degradable material PNIPAAm can postpone the degradation of HA more effectively than degradable material MC.PNIPAAm prepared with 5 kGy dose radiation,MBAAm/NIPAAm(M/M)=0.015,monomer concentration=3% produced XLHA-PNIPAAm with slowest enzymatic biodegradability.DSC results showed that temperature-sensitivity of the XLHA-PNIPAAm was more stable than that of XLHA-MC.Two composite hydrogels were qualified in cytotoxicity and hemolysis tests and the biocompatibility of XLHA-PNIPAAm hydrogel showed better than XLHA-MC hydrogel.展开更多
Conducting hydrogels have garnered significant interest in the field of wearable electronics.However,simultaneously achieving high transparency,high conductivity,strong adhesion,and self-healing ability within a short...Conducting hydrogels have garnered significant interest in the field of wearable electronics.However,simultaneously achieving high transparency,high conductivity,strong adhesion,and self-healing ability within a short time remains a major challenge.In this study,a multifunctional mussel-inspired hydrogel was synthesized in only 5 min,with polydopamine(PDA)-polypyrrole(Ppy)-polyaniline(PANi)and poly(vinyl alcohol)(PVA)nanoparticles incorporated into the polyacrylamide(PAM)network.The resulting hydrogel exhibited high transparency(about 90% light transmission in the range of 400-800 nm),high conductivity((95.4±0.4)×10^(-4)S/cm),tensile strength(32.60±1.03 k Pa),strain at break(904.46%±11.50%),and adhesive strength(30-60 k Pa).It also demonstrated rapid self-healing properties(about 48% strength recovery within 1h at 50℃)and water-dependent shape memory behavior.As a wearable strain sensor,the hydrogel successfully detected finger flexion,wrist movements,facial expression changes,and breathing with high sensitivity and stability.The calculated gauge factor(GF)was 7.44±0.31,which is higher than that of many previously reported hydrogels.Compared with previous oyster-inspired or Ppy-based hydrogels,our system showed a much shorter synthesis time,higher transparency,and enhanced multifunctionality.These findings highlight the potential of the proposed hydrogel for next-generation flexible electronics,e-skin,and biomedical monitoring devices.展开更多
This study presents a thorough investigation into the use of single and twin-tailed cationic and anionic surfactant-modified chitosan(SMCS)hydrogel beads as effective adsorbents for the elimination of hazardous polycy...This study presents a thorough investigation into the use of single and twin-tailed cationic and anionic surfactant-modified chitosan(SMCS)hydrogel beads as effective adsorbents for the elimination of hazardous polycyclic aromatic hydrocarbons(PAHs)from aqueous solutions.The Chitosan(CS)hydrogel beads were modified with single/twin-tailed anionic surfactants,sodium dodecyl sulfate(SDS)and sodium bis(2-ethylhexyl)sulfosuccinate(AOT),and cationic surfactants,dodecyltrimethylammonium bromide(DTAB)and didodecyldimethylammonium bromide(DDAB),to enhance their adsorption capacity of PAHs.The CS and SMCS beads were evaluated for their structural,mechanical,and adsorption properties using a range of techniques,including infrared spectroscopy(IR),energy-dispersive X-ray spectroscopy(EDX),rheometry,and field emission scanning electron microscopy(FESEM).Adsorption experiments of naphthalene(Nap),acenaphthene(Ace),and phenanthrene(Phe)on SMCS beads demonstrate that they have significantly higher adsorption capacities than CS beads,due to increase in hydrophobic interactions.Adsorption capacity followed the trend,Phen>Ace>Nap for all the beads revealing that twin-tailed SMCS bead possess much higher adsorption capacities(Qmax)compared to single-tailed SMCS beads.For twin tailed surfactants,the maximum adsorption capacities for Nap,Ace and Phe varied as CS-AOT(CS-DDAB):430.0(323.8)611.60(538.18)633.39(536.99)mg/g respectively,outperforming other reported hydrogel beads.The study highlights the simplicity,eco-friendliness,and enhanced performance of surfactant modification for developing high-efficiency adsorbents,paving the way for cost-effective solutions in water re-mediation.展开更多
By combining the merits of radiative cooling(RC)and evaporation cooling(EC),radiative coupled evaporative cooling(REC)has attracted considerable attention for sub-ambient cooling purposes.However,for outdoor devices,t...By combining the merits of radiative cooling(RC)and evaporation cooling(EC),radiative coupled evaporative cooling(REC)has attracted considerable attention for sub-ambient cooling purposes.However,for outdoor devices,the interior heating power would increase the working temperature and fire risk,which would suppress their above-ambient heat dissipation capabilities and passive water cycle properties.In this work,we introduced a REC design based on an all-in-one photonic hydrogel for above-ambient heat dissipation and flame retardancy.Unlike conventional design RC film for heat dissipation with limited cooling power and fire risk,REC hydrogel can greatly improve the heat dissipation performance in the daytime with a high workload,indicating a 12.0℃lower temperature than the RC film under the same conditions in the outdoor experiment.In the nighttime with a low workload,RC-assisted adsorption can improve atmospheric water harvesting to ensure EC in the daytime.In addition,our REC hydrogel significantly enhanced flame retardancy by absorbing heat without a corresponding temperature rise,thus mitigating fire risks.Thus,our design shows a promising solution for the thermal management of outdoor devices,delivering outstanding performance in both heat dissipation and flame retardancy.展开更多
Solar-driven interfacial desalination(SID)offers a sustainable route for freshwater production,yet its long-term performance is compromised by salt crystallization and microbial fouling under complex marine conditions...Solar-driven interfacial desalination(SID)offers a sustainable route for freshwater production,yet its long-term performance is compromised by salt crystallization and microbial fouling under complex marine conditions.Zwitterionic polymers offer promising nonfouling capabilities,but current zwitterionic hydrogel-based solar evaporators(HSEs)suffer from inadequate hydration and salt vulnerability.Inspired by the natural marine environmental adaptive characteristics of saltwater fish,we report a superhydrated zwitterionic poly(trimethylamine N-oxide,PTMAO)/polyacrylamide(PAAm)/polypyrrole(PPy)hydrogel(PTAP)with dedicated water channels for efficient,durable,and nonfouling SID.The directly linked N⁺and O⁻groups in PTMAO establish a robust hydration shell that facilitates rapid water transport while resisting salt and microbial adhesion.Integrated PAAm and PPy networks enhance mechanical strength and photothermal conversion.PTAP achieves a high evaporation rate of 2.35 kg m^(−2)h^(−1)under 1 kW m^(–2)in 10 wt%NaCl solution,maintaining stable operation over 100 h without salt accumulation.Furthermore,PTAP effectively resists various foulants including proteins,bacterial,and algal adhesion.Molecular dynamics simulations reveal that the exceptional hydration capacity supports its nonfouling properties.This work advances the development of nonfouling HSEs for sustainable solar desalination in real-world marine environments.展开更多
Octopuses,due to their flexible arms,marvelous adaptability,and powerful suckers,are able to effortlessly grasp and disengage various objects in the marine surrounding without causing devastation.However,manipulating ...Octopuses,due to their flexible arms,marvelous adaptability,and powerful suckers,are able to effortlessly grasp and disengage various objects in the marine surrounding without causing devastation.However,manipulating delicate objects such as soft and fragile foods underwater require gentle contact and stable adhesion,which poses a serious challenge to now available soft grippers.Inspired by the sucker infundibulum structure and flexible tentacles of octopus,herein we developed a hydraulically actuated hydrogel soft gripper with adaptive maneuverability by coupling multiple hydrogen bond-mediated supramolecular hydrogels and vat polymerization three-dimensional printing,in which hydrogel bionic sucker is composed of a tunable curvature membrane,a negative pressure cavity,and a pneumatic chamber.The design of the sucker structure with the alterable curvature membrane is conducive to realize the reliable and gentle switchable adhesion of the hydrogel soft gripper.As a proof-of-concept,the adaptive hydrogel soft gripper is capable of implement diversified underwater tasks,including gingerly grasping fragile foods like egg yolks and tofu,as well as underwater robots and vehicles that station-keeping and crawling based on switchable adhesion.This study therefore provides a transformative strategy for the design of novel soft grippers that will render promising utilities for underwater exploration soft robotics.展开更多
Bone regeneration for non-load-bearing defects remains a significant clinical challenge requiring advanced biomaterials and cellular strategies.Adiposederived mesenchymal stem cells(AD-MSCs)have garnered significant i...Bone regeneration for non-load-bearing defects remains a significant clinical challenge requiring advanced biomaterials and cellular strategies.Adiposederived mesenchymal stem cells(AD-MSCs)have garnered significant interest in bone tissue engineering(BTE)because of their abundant availability,minimally invasive harvesting procedures,and robust differentiation potential into osteogenic lineages.Unlike bone marrow-derived mesenchymal stem cells,AD-MSCs can be easily obtained in large quantities,making them appealing alternatives for therapeutic applications.This review explores hydrogels containing polymers,such as chitosan,collagen,gelatin,and hyaluronic acid,and their composites,tailored for BTE,and emphasizes the importance of these hydrogels as scaffolds for the delivery of AD-MSCs.Various hydrogel fabrication techniques and biocompatibility assessments are discussed,along with innovative modifications to enhance osteogenesis.This review also briefly outlines AD-MSC isolation methods and advanced embedding techniques for precise cell placement,such as direct encapsulation and three-dimensional bioprinting.We discuss the mechanisms of bone regeneration in the AD-MSC-laden hydrogels,including osteoinduction,vascularization,and extracellular matrix remodeling.We also review the preclinical and clinical applications of AD-MSC-hydrogel systems,emphasizing their success and limitations.In this review,we provide a comprehensive overview of AD-MSC-based hydrogel systems to guide the development of effective therapies for bone regeneration.展开更多
Peripheral nerve injury causes severe neuroinflammation and has become a global medical challenge.Previous research has demonstrated that porcine decellularized nerve matrix hydrogel exhibits excellent biological prop...Peripheral nerve injury causes severe neuroinflammation and has become a global medical challenge.Previous research has demonstrated that porcine decellularized nerve matrix hydrogel exhibits excellent biological properties and tissue specificity,highlighting its potential as a biomedical material for the repair of severe peripheral nerve injury;however,its role in modulating neuroinflammation post-peripheral nerve injury remains unknown.Here,we aimed to characterize the anti-inflammatory properties of porcine decellularized nerve matrix hydrogel and their underlying molecular mechanisms.Using peripheral nerve injury model rats treated with porcine decellularized nerve matrix hydrogel,we evaluated structural and functional recovery,macrophage phenotype alteration,specific cytokine expression,and changes in related signaling molecules in vivo.Similar parameters were evaluated in vitro using monocyte/macrophage cell lines stimulated with lipopolysaccharide and cultured on porcine decellularized nerve matrix hydrogel-coated plates in complete medium.These comprehensive analyses revealed that porcine decellularized nerve matrix hydrogel attenuated the activation of excessive inflammation at the early stage of peripheral nerve injury and increased the proportion of the M2 subtype in monocytes/macrophages.Additionally,porcine decellularized nerve matrix hydrogel negatively regulated the Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-κB axis both in vivo and in vitro.Our findings suggest that the efficacious anti-inflammatory properties of porcine decellularized nerve matrix hydrogel induce M2 macrophage polarization via suppression of the Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-κB pathway,providing new insights into the therapeutic mechanism of porcine decellularized nerve matrix hydrogel in peripheral nerve injury.展开更多
The major aim of stroke therapy is to stimulate brain repair and improve behavioral recovery after cerebral ischemia.One option is to stimulate endogenous neurogenesis in the subventricular zone and direct the newly f...The major aim of stroke therapy is to stimulate brain repair and improve behavioral recovery after cerebral ischemia.One option is to stimulate endogenous neurogenesis in the subventricular zone and direct the newly formed neurons to the damaged area.However,only a small percentage of these neurons survive,and many do not reach the damaged area,possibly because the corpus callosum impedes the migration of subventricular zone-derived stem cells into the lesioned cortex.A second major obstacle to stem cell therapy is the strong inflammatory reaction induced by cerebral ischemia,whereby the associated phagocytic activity of brain macrophages removes both therapeutic cells and/or cell-based drug carriers.To address these issues,neurogenesis was electrically stimulated in the subventricular zone,followed by isolation of proliferating cells,including newly formed neurons,which were subsequently mixed with a nutritional hydrogel.This mixture was then transferred to the stroke cavity of day 14 post-stroke mice.We found that the performance of the treated animals improved in behavioral tests,including novel object,open field,hole board,grooming,and“time-to-feel”adhesive tape tests.Furthermore,immunostaining revealed that the stem cell marker nestin,the neuroepithelial marker Mash1,and the immature neuronal marker doublecortin-positive cells survived in the transplanted area for 2 weeks,possibly due to reduced phagocytic activity and supportive angiogenesis.These results clearly indicate that the transplantation of committed subventricular zone stem cells combined with a protective nutritional gel directly into the infarct cavity after the peak of stroke-induced neuroinflammation represents a feasible approach to improve neurorestoration after cerebral ischemia.展开更多
基金the National Natural Science Foundation of China (No. 20276065).
文摘Temperature-sensitive hydrogel—poly(N-isopropyl acrylamide) (PNIPA) was prepared and applied to protein refolding. PNIPA gel disks and gel particles were synthesized by the solution polymerization and inverse suspension polymerization respectively. The swelling kinetics of the gels was also studied. With these prepared PNIPA gels, the model protein lysozyme was renatured. Within 24h, PNIPA gel disks improved the yield of lysozyme activity by 49.3% from 3375.2U·mg^-1 to 5038.8U·mg^-1. With the addition of faster response PNIPA gel beads, the total lysozyme activity recovery was about 68.98% in 3h, as compared with 42.03% by simple batch dilution. The novel refolding system with PNIPA enables efficient refolding especially at high protein concentrations. Discussion about the mechanism revealed that when PNIPA gels were added into the refolding buffer, the hydrophobic interactions between denatured proteins and polymer gels could prevent the aggregation of refolding intermediates, thus enhanced the protein renaturation.
基金Research Fund for the Doctoral Programof Higher Education of China(No.20050058006)
文摘In this study,the hydrogels composites with coatings based on a temperature-sensitive linear copolymer of N-tert-butylacrylamide(NTBA)and acrylamide(AAm)on cotton fabrics have been developed.The cotton fabrics were coated using aqueous solution of the linear copolymer,1,2,3,4-butanetertracarboxylic acid(BTCA)as a cross-linker and sodium hypophosphite(SHP)as a catalyst,followed by drying and curing.The effects of cross-linking reaction conditions in coating process on water-impermeable ability of coated cotton fabrics were investigated in detail.The results indicate that the coated fabrics have temperature sensitivity.The coatings of poly(NTBA-co-AAm)hydrogels were bonded on the surface of the cotton fabrics,as verified by SEM and optical microscopy,which gave the water-impermeable ability to the hydrogels composites.Moreover,the hydrogels formed in the coating process also identified that - COOH of BTCA reacted with -NH2 in the linear polymer and formed three-dimensional network hydrogels.FTIR and XPS were used to characterize the cross-linking reaction of - COOH of BTCA and - OH of cellulose.
文摘The swelling behavior of a temperature-sensitive poly-N-isopropylacrylamide(PNIPAM) hydrogel circular cylinder is studied subjected to combined extension-torsion and varied temperature. In this regard, a semi-analytical solution is proposed for general combined loading. A finite element(FE) analysis is conducted, subjecting a hydrogel cylinder to the combined extension-torsion and the varied temperature to evaluate the validity and accuracy of the solution. A user-defined UHYPER subroutine is developed and verified under free and constrained swelling conditions. The FE results illustrate excellent agreement with the semi-analytical solution. Due to the complexity of the problem, some compositions and applied loading factors are analyzed. It is revealed that for larger cross-linked density and larger ending temperature, the cylinder yields higher stresses and smaller radial swelling deformation. Besides, the radial and hoop stresses increase by applying larger twist and axial stretch. The hoop stresses intersect at approximately R/Rout = 0.58, where the hoop stress vanishes. Besides, the axial force has direct and inverse relationships with the axial stretch and the twist, respectively. However, the resultant torsional moment behavior is complex, and the position of the maximum point varies significantly by altering the axial stretch and the twist.
文摘A kind of novel copolymer hydrogel of poly（N, N-dimethylaminoethyl methacrylate-co-N-isopropylacrylamide） （poly[DMAEMA/NIPAAm]） was synthesized by the initiation of K2S2O8, N, N＇-methylene-bis（acrylamide） （Bis） was used as the crosslinker. The effects of monomer content, pH and temperature on swelling ratio of the hydrogel were investigated; the thermo-sensitivity in deionized water and in physiological saline was determined. It showed that the swelling ratio of the hydrogel could be changed by changing the temperature or pH alternately. Both swelling ratio and LCST （Lower Critical Solution Temperature） of the hydrogel decreased with the increase of NIPAAm in the co-polymer content.
基金supported by the National Natural Science Foundation of China(No.51508233)the Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment
文摘Nanoscale zero-valent iron(n ZVI) particles supported on a porous, semi-interpenetrating(semi-IPN), temperature-sensitive composite hydrogel(PNIPAm-PHEMA). n ZVI@PNIPAmPHEMA, was successfully synthesized and characterized by FT-IR, SEM, EDS, XRD and the weighing method. The loading of nZVI was 0.1548 ± 0.0015 g/g and the particle size was30–100 nm. NZVI was uniformly dispersed on the pore walls inside the PNIPAm-PHEMA.Because of the well-dispersed n ZVI, the highly porous structure, and the synergistic effect of PNIPAm-PHEMA, nZVI@PNIPAm-PHEMA showed excellent reductive activity and wide p H applicability. 95% of 4-NP in 100 m L of 400 mg/L 4-NP solution with initial p H 3.0–9.0 could be completely reduced into 4-AP by about 0.0548 g of fresh supported n ZVI at 18–25 °C under stirring(110 r/min) within 45 min reaction time. A greater than 99% 4-NP degradation ratio was obtained when the initial p H was 5.0–9.0. The reduction of 4-NP by nZVI@PNIPAm-PHEMA was in agreement with the pseudo-first-order kinetics model with Kobsvalues of 0.0885–0.101 min-1.NZVI@PNIPAm-PHEMA was able to be recycled, and about 85% degradation ratio of 4-NP was obtained after its sixth reuse cycle. According to the temperature sensitivity of PNIPAmPHEMA, n ZVI@PNIPAm-PHEMA exhibited very good storage stability, and about 88.9%degradation ratio of 4-NP was obtained after its storage for 30 days. The hybrid reducer was highly efficient for the reduction of 2-NP, 3-NP, 2-chloro-4-nitrophenol and 2-chloro-4-nitrophenol. Our results suggest that PNIPAm-PHEMA could be a good potential carrier, with n ZVI@PNIPAm-PHEMA having potential value in the application of reductive degradation of nitrophenol pollutants.
基金National Natural Science Foundation of China (81972886 and 82172736)Shanghai Health and Family Planning Commission Fund (202240235)+1 种基金Natural Science Foundation of Shanghai (21ZR1451400)Shanghai Jiading District Health and Family Planning Commission Fund (2021-KY-10)。
文摘Atherosclerosis remains a major cause of morbidity and mortality worldwide.Intraplaque neovascularization critically promotes atherosclerotic progression and instability.Vascular endothelial growth factor A(VEGFA)stimulates aberrant microvessel growth in plaques by inducing endothelial cell proliferation and migration.Pigment epithelium-derived factor(PEDF)potently inhibits VEGFA-dependent neovascularization.This study introduces a thermosensitive hydrogel(PFSgel)developed from poloxamer 407(F127)and sodium alginate(SA)to deliver PEDF locally to atherosclerotic lesions.The PFSgel demonstrated a suitable liquid-solid transition at body temperature(37°C),then forming a stable 3D network structure after SA gelling with the Ca2+in the physiological environment which contributed to the character of controlled release.Rheological analysis confirmed its phase transition temperature of 28.7°C and notable self-healing properties,making it ideal for dynamic vascular environments.In vitro experiments showed that PFSgel could suppress VEGFA-induced endothelial cells’proliferation and migration through modulation of CD31 and MMP-2/MMP-9 signaling.Notably,in vivo degradation test validated the controlled release pattern of PFSgel.In Apoedeficient atherosclerotic mice,ultrasound-guided PFSgel injection onto the abdominal aorta enabled gradual in situ release of encapsulated PEDF.This effectively reduced plaque burden,neovascularization,and luminal stenosis,even with exogenous VEGFA administration.Histological analyses confirmed reduced lipid deposition,plaque area,and neovascularization within plaques.Overall,this novel in situforming PEDF delivery platform enables targeted suppression of pathological neovascularization via CD31 and MMP-2/MMP-9 pathways,representing a promising approach to stabilize high-risk plaques by intervening against VEGFA-dependent neovascularization.
基金financially supported by the National Natural Science Foundation of China(No.52173301)International Science and Technology Cooperation Project of Sichuan Province(No.2022YFH0019)Innovative Research Team of Southwest Petroleum University(No.2017CXTD01)。
文摘Flexible hydrogels have shown promise as strain sensors in medical monitoring,human motion detection and intelligent robotics.For a hydrogel strain sensor,certain challenges need to be urgently addressed for practical applications,such as the damage caused by external effects,leading to equipment failure,and the inability to perceive ambient temperature,resulting in single functionality.Herein,a stretchable,self-healing and dual temperature-strain sensitive hydrogel,with a physically-crosslinked network,is designed by constructing multiple dynamic reversible bonds.Graphene oxide(GO)and iron ions(Fe^(3+))act as dynamic bridges in the cross-linked network and are mediated by the covalent and hydrogen bonding,rendering excellent stretchability to the hydrogel.The reversible features of coordination interactions and hydrogen interactions endow excellent recoverability and self-healing properties.Moreover,the incorporated N-isopropyl acrylamide(NIPAM)provides excellent temperature responsiveness to the hydrogel,facilitating the detection of external temperature changes.Meanwhile,the hydrogels exhibited strain-sensitivity,with a wide working range of 1%-300%,fast response and electrical stability,which can be used as flexible sensors to monitor body motions,e.g.,speaking and the bending of finger,wrist,elbow and knee.Overall,the hydrogel possesses dual sensory capabilities,combining external temperature and strain,for potential applications in wearable multifunctional sensing devices.
基金funded by the Beijing Municipal Natural Science Foundation,China(7202147 and 7172072,China)
文摘Post-traumatic stress disorder(PTSD)is a psychiatric disease that seriously affects brain function.Currently,selective serotonin reuptake inhibitors(SSRIs)are used to treat PTSD clinically but have decreased efficiency and increased side effects.In this study,nasal cannabidiol inclusion complex temperature-sensitive hydrogels(CBD TSGs)were prepared and evaluated to treat PTSD.Mice model of PTSD was established with conditional fear box.CBD TSGs could significantly improve the spontaneous behavior,exploratory spirit and alleviate tension in open field box,relieve anxiety and tension in elevated plus maze,and reduce the freezing time.Hematoxylin and eosin and c-FOS immunohistochemistry slides showed that the main injured brain areas in PTSD were the prefrontal cortex,amygdala,and hippocampus CA1.CBD TSGs could reduce the level of tumor necrosis factor-a caused by PTSD.Western blot analysis showed that CBD TSGs increased the expression of the 5-HT1 A receptor.Intranasal administration of CBD TSGs was more efficient and had more obvious brain targeting effects than oral administration,as evidenced by the pharmacokinetics and brain tissue distribution of CBD TSGs.Overall,nasal CBD TSGs are safe and effective and have controlled release.There are a novel promising option for the clinical treatment of PTSD.
文摘Semi-interpenetrating （semi-IPNs） hydrogels containing biocompatible silk sericin （SS） and poly（N-isopropylacrylamide）（PNIPAM） were prepared as novel cellular matrices. Their maximum swelling degree and basic characteristics for biomedical applications such as mouse ？broblasts （L929） cell proliferation and desorption were investigated. The results showed that the incorporation of high hydrophilic SS into PNIPAM hydrogel increased the maximum swelling degree of the semi-IPNs hydrogels, and the adhesion and growth of the L929 on semi-IPNs hydrogels were at least comparable to, or even better than, that on conventional PNIPAM hydrogel. In addition, L929 cells were found to detach from the hydrogels surface naturally by controlling environmental temperature. These results suggest great potential of semi-IPNs hydrogels in tissue engineering.
基金financially supported by the National Natural Science Foundation of China(Nos.52273009 and 21674037).
文摘Insulin is an essential and versatile protein taking part in the control of blood glucose levels and protein anabolism.However,under prolonged storage or high temperature stress,insulin tends to unfold and aggregate into toxic amyloid fibrils,leading to loss of physiological function.Inspired by natural chaperones,a series of temperature-sensitive polycaprolactone-based micelles were designed to prevent insulin from deactivation.The micelles were fabricated through the self-assembly of amphiphilic copolymers of methoxy poly(ethylene glycol)-poly(4-diethylformamide caprolactone-co-caprolactone)(mPEG_(17)-P(DECL-co-CL)),which had a regular spherical morphology with particle sizes of about 100 nm.In addition,the lower critical solution temperature(LCST)of the micelles could be tuned to 9 and 29℃by changing the ratio of DECL to CL.Benefiting from the temperature-sensitivity of DECL segment,the binding ability of micelles to insulin could be modulated by changing the temperature.Above LCST,micelles effectively inhibited insulin aggregation and protected it from thermal inactivation due to the strong binding ability between the hydrophobic segment DECL and insulin.Below LCST,DECL segment returned to hydrophilic and bound weakly with insulin,leading to the release of insulin and assisting in its recovery of secondary structure.Thus,these temperature-sensitive micelles provided an effective strategy for insulin protection.
基金Supported by National Natural Science Foundation of China(52404010).
文摘A temperature-sensitive mud cake remover(G315)was developed using ethyl lactate as the primary component.Based on this,a temperature-sensitive acidic completion fluid(CF-G315)was formulated.Core evaluation tests,mud cake dissolution tests and corrosion tests were conducted to analyze the mud cake removal performance of G315,the removal efficiency of CF-G315,and its ability to modify the near-wellbore reservoirs,corrosion to casing and hydrolysis performance.Results indicate that ethyl lactate in G315 exhibits weak acidity at room temperature and decomposes into lactic acid under high temperatures.The lactic acid reacts with the calcium carbonate in the mud cake,generating bubbles that dislodge the mud cake and form soluble salts that are subsequently removed by fluid flow,thereby ensuring effective mud cake clearance.CF-G315 removes mud cake efficiently and enhances near-wellbore reservoir permeability.It demonstrates low corrosivity and environmental compatibility,contributing to equipment safety,simplified operational procedures and reduced operational risks.CF-G315 is promising for application in scenarios such as horizontal wells,open-hole completions and gravel pack completions.
基金The Nattional Key Scientific Program-Nanoscience and Nanotechnologygrant number:2009CB930000
文摘To get a sort of new scaffold material for soft tissue reconstruction,we have prepared XLHA-PNIPAAm and XLHA-MC injectable hydrogels through blending crosslinked HA(XLHA) and two temperature-sensitive materials differed in degradation poly(N-isopropylacrylamide)(PNIPAAm) and methylcellulose(MC),respectively.We tested the injectablility,enzymatic biodegradability,temperature-sensitivity,structure cytotoxicity and hemolysis of the two injectable hydrogels.Our research has successfully obtained the preparation condition of XLHA-PNIPAAm injectable hydrogel,and verified that adding non-degradable material PNIPAAm can postpone the degradation of HA more effectively than degradable material MC.PNIPAAm prepared with 5 kGy dose radiation,MBAAm/NIPAAm(M/M)=0.015,monomer concentration=3% produced XLHA-PNIPAAm with slowest enzymatic biodegradability.DSC results showed that temperature-sensitivity of the XLHA-PNIPAAm was more stable than that of XLHA-MC.Two composite hydrogels were qualified in cytotoxicity and hemolysis tests and the biocompatibility of XLHA-PNIPAAm hydrogel showed better than XLHA-MC hydrogel.
文摘Conducting hydrogels have garnered significant interest in the field of wearable electronics.However,simultaneously achieving high transparency,high conductivity,strong adhesion,and self-healing ability within a short time remains a major challenge.In this study,a multifunctional mussel-inspired hydrogel was synthesized in only 5 min,with polydopamine(PDA)-polypyrrole(Ppy)-polyaniline(PANi)and poly(vinyl alcohol)(PVA)nanoparticles incorporated into the polyacrylamide(PAM)network.The resulting hydrogel exhibited high transparency(about 90% light transmission in the range of 400-800 nm),high conductivity((95.4±0.4)×10^(-4)S/cm),tensile strength(32.60±1.03 k Pa),strain at break(904.46%±11.50%),and adhesive strength(30-60 k Pa).It also demonstrated rapid self-healing properties(about 48% strength recovery within 1h at 50℃)and water-dependent shape memory behavior.As a wearable strain sensor,the hydrogel successfully detected finger flexion,wrist movements,facial expression changes,and breathing with high sensitivity and stability.The calculated gauge factor(GF)was 7.44±0.31,which is higher than that of many previously reported hydrogels.Compared with previous oyster-inspired or Ppy-based hydrogels,our system showed a much shorter synthesis time,higher transparency,and enhanced multifunctionality.These findings highlight the potential of the proposed hydrogel for next-generation flexible electronics,e-skin,and biomedical monitoring devices.
基金the Department of Science and Technology(DST),Govt.of India for providing funds under the FIST program and PURSE grant vide No.SR/PURSE/2020/31 to the department of Chemistry,University of Kashmir.
文摘This study presents a thorough investigation into the use of single and twin-tailed cationic and anionic surfactant-modified chitosan(SMCS)hydrogel beads as effective adsorbents for the elimination of hazardous polycyclic aromatic hydrocarbons(PAHs)from aqueous solutions.The Chitosan(CS)hydrogel beads were modified with single/twin-tailed anionic surfactants,sodium dodecyl sulfate(SDS)and sodium bis(2-ethylhexyl)sulfosuccinate(AOT),and cationic surfactants,dodecyltrimethylammonium bromide(DTAB)and didodecyldimethylammonium bromide(DDAB),to enhance their adsorption capacity of PAHs.The CS and SMCS beads were evaluated for their structural,mechanical,and adsorption properties using a range of techniques,including infrared spectroscopy(IR),energy-dispersive X-ray spectroscopy(EDX),rheometry,and field emission scanning electron microscopy(FESEM).Adsorption experiments of naphthalene(Nap),acenaphthene(Ace),and phenanthrene(Phe)on SMCS beads demonstrate that they have significantly higher adsorption capacities than CS beads,due to increase in hydrophobic interactions.Adsorption capacity followed the trend,Phen>Ace>Nap for all the beads revealing that twin-tailed SMCS bead possess much higher adsorption capacities(Qmax)compared to single-tailed SMCS beads.For twin tailed surfactants,the maximum adsorption capacities for Nap,Ace and Phe varied as CS-AOT(CS-DDAB):430.0(323.8)611.60(538.18)633.39(536.99)mg/g respectively,outperforming other reported hydrogel beads.The study highlights the simplicity,eco-friendliness,and enhanced performance of surfactant modification for developing high-efficiency adsorbents,paving the way for cost-effective solutions in water re-mediation.
基金financially supported by the Science and Technology Innovation Program of Hunan Province(2024RC3003)the Central South University Innovation-Driven Research Programme(2023CXQD012)the Initiative for Sustainable Energy for its financial support。
文摘By combining the merits of radiative cooling(RC)and evaporation cooling(EC),radiative coupled evaporative cooling(REC)has attracted considerable attention for sub-ambient cooling purposes.However,for outdoor devices,the interior heating power would increase the working temperature and fire risk,which would suppress their above-ambient heat dissipation capabilities and passive water cycle properties.In this work,we introduced a REC design based on an all-in-one photonic hydrogel for above-ambient heat dissipation and flame retardancy.Unlike conventional design RC film for heat dissipation with limited cooling power and fire risk,REC hydrogel can greatly improve the heat dissipation performance in the daytime with a high workload,indicating a 12.0℃lower temperature than the RC film under the same conditions in the outdoor experiment.In the nighttime with a low workload,RC-assisted adsorption can improve atmospheric water harvesting to ensure EC in the daytime.In addition,our REC hydrogel significantly enhanced flame retardancy by absorbing heat without a corresponding temperature rise,thus mitigating fire risks.Thus,our design shows a promising solution for the thermal management of outdoor devices,delivering outstanding performance in both heat dissipation and flame retardancy.
基金supported by National Natural Science Foundation of China(22209036,U23A20119)Hebei Provincial Natural Science Foundation,Excellent Youth Project(E2023202069)+1 种基金National Key R&D Program of China(2024YFF0506000,2024YFB4609100)Fundamental Research Foundation from Hebei University of Technology(424132016,282021485).
文摘Solar-driven interfacial desalination(SID)offers a sustainable route for freshwater production,yet its long-term performance is compromised by salt crystallization and microbial fouling under complex marine conditions.Zwitterionic polymers offer promising nonfouling capabilities,but current zwitterionic hydrogel-based solar evaporators(HSEs)suffer from inadequate hydration and salt vulnerability.Inspired by the natural marine environmental adaptive characteristics of saltwater fish,we report a superhydrated zwitterionic poly(trimethylamine N-oxide,PTMAO)/polyacrylamide(PAAm)/polypyrrole(PPy)hydrogel(PTAP)with dedicated water channels for efficient,durable,and nonfouling SID.The directly linked N⁺and O⁻groups in PTMAO establish a robust hydration shell that facilitates rapid water transport while resisting salt and microbial adhesion.Integrated PAAm and PPy networks enhance mechanical strength and photothermal conversion.PTAP achieves a high evaporation rate of 2.35 kg m^(−2)h^(−1)under 1 kW m^(–2)in 10 wt%NaCl solution,maintaining stable operation over 100 h without salt accumulation.Furthermore,PTAP effectively resists various foulants including proteins,bacterial,and algal adhesion.Molecular dynamics simulations reveal that the exceptional hydration capacity supports its nonfouling properties.This work advances the development of nonfouling HSEs for sustainable solar desalination in real-world marine environments.
基金the financial support from the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB0470303)the National Key Research and Development Program of China (2022YFB4600101)+5 种基金the National Natural Science Foundation of China (52175201)the Research Program of Science and Technology Department of Gansu Province (24JRRA059, 24JRRA044, and 24YFFA014)the Science Fund of Shandong Laboratory of Advanced Materials and Green Manufacturing at Yantai (AMGM2024F12)the Major Program (ZYFZFX-2) of the Lanzhou Institute of Chemical Physics, CASthe Special Research Assistant Project of the Chinese Academy of Sciencesthe Oasis Scholar of Shihezi University
文摘Octopuses,due to their flexible arms,marvelous adaptability,and powerful suckers,are able to effortlessly grasp and disengage various objects in the marine surrounding without causing devastation.However,manipulating delicate objects such as soft and fragile foods underwater require gentle contact and stable adhesion,which poses a serious challenge to now available soft grippers.Inspired by the sucker infundibulum structure and flexible tentacles of octopus,herein we developed a hydraulically actuated hydrogel soft gripper with adaptive maneuverability by coupling multiple hydrogen bond-mediated supramolecular hydrogels and vat polymerization three-dimensional printing,in which hydrogel bionic sucker is composed of a tunable curvature membrane,a negative pressure cavity,and a pneumatic chamber.The design of the sucker structure with the alterable curvature membrane is conducive to realize the reliable and gentle switchable adhesion of the hydrogel soft gripper.As a proof-of-concept,the adaptive hydrogel soft gripper is capable of implement diversified underwater tasks,including gingerly grasping fragile foods like egg yolks and tofu,as well as underwater robots and vehicles that station-keeping and crawling based on switchable adhesion.This study therefore provides a transformative strategy for the design of novel soft grippers that will render promising utilities for underwater exploration soft robotics.
文摘Bone regeneration for non-load-bearing defects remains a significant clinical challenge requiring advanced biomaterials and cellular strategies.Adiposederived mesenchymal stem cells(AD-MSCs)have garnered significant interest in bone tissue engineering(BTE)because of their abundant availability,minimally invasive harvesting procedures,and robust differentiation potential into osteogenic lineages.Unlike bone marrow-derived mesenchymal stem cells,AD-MSCs can be easily obtained in large quantities,making them appealing alternatives for therapeutic applications.This review explores hydrogels containing polymers,such as chitosan,collagen,gelatin,and hyaluronic acid,and their composites,tailored for BTE,and emphasizes the importance of these hydrogels as scaffolds for the delivery of AD-MSCs.Various hydrogel fabrication techniques and biocompatibility assessments are discussed,along with innovative modifications to enhance osteogenesis.This review also briefly outlines AD-MSC isolation methods and advanced embedding techniques for precise cell placement,such as direct encapsulation and three-dimensional bioprinting.We discuss the mechanisms of bone regeneration in the AD-MSC-laden hydrogels,including osteoinduction,vascularization,and extracellular matrix remodeling.We also review the preclinical and clinical applications of AD-MSC-hydrogel systems,emphasizing their success and limitations.In this review,we provide a comprehensive overview of AD-MSC-based hydrogel systems to guide the development of effective therapies for bone regeneration.
基金supported by the Shenzhen Hong Kong Joint Funding Project,No.SGDX20230116093645007(to LY)the Shenzhen Science and Technology Innovation Committee International Cooperation Project,No.GJHZ20200731095608025(to LY)+7 种基金Shenzhen Development and Reform Commission’s Intelligent Diagnosis,Treatment and Prevention of Adolescent Spinal Health Public Service Platform,No.S2002Q84500835(to LY)Shenzhen Medical Research Fund,No.B2303005(to LY)Team-based Medical Science Research Program,No.2024YZZ02(to LY)Zhejiang Provincial Natural Science Foundation of China,No.LWQ20H170001(to RL)Basic Research Project of Shenzhen Science and Technology from Shenzhen Science and Technology Innovation Commission,No.JCYJ20210324103010029(to BY)Shenzhen Second People’s Hospital Clinical Research Fund of Guangdong Province High-level Hospital Construction Project,Nos.2023yjlcyj029(to BY),2023yjlcyj021(to LL)Guangdong Basic and Applied Basic Research Foundation,No.2022A1515110679(to LL)China Postdoctoral Science Foundation,No.2022M722203(to GL).
文摘Peripheral nerve injury causes severe neuroinflammation and has become a global medical challenge.Previous research has demonstrated that porcine decellularized nerve matrix hydrogel exhibits excellent biological properties and tissue specificity,highlighting its potential as a biomedical material for the repair of severe peripheral nerve injury;however,its role in modulating neuroinflammation post-peripheral nerve injury remains unknown.Here,we aimed to characterize the anti-inflammatory properties of porcine decellularized nerve matrix hydrogel and their underlying molecular mechanisms.Using peripheral nerve injury model rats treated with porcine decellularized nerve matrix hydrogel,we evaluated structural and functional recovery,macrophage phenotype alteration,specific cytokine expression,and changes in related signaling molecules in vivo.Similar parameters were evaluated in vitro using monocyte/macrophage cell lines stimulated with lipopolysaccharide and cultured on porcine decellularized nerve matrix hydrogel-coated plates in complete medium.These comprehensive analyses revealed that porcine decellularized nerve matrix hydrogel attenuated the activation of excessive inflammation at the early stage of peripheral nerve injury and increased the proportion of the M2 subtype in monocytes/macrophages.Additionally,porcine decellularized nerve matrix hydrogel negatively regulated the Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-κB axis both in vivo and in vitro.Our findings suggest that the efficacious anti-inflammatory properties of porcine decellularized nerve matrix hydrogel induce M2 macrophage polarization via suppression of the Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-κB pathway,providing new insights into the therapeutic mechanism of porcine decellularized nerve matrix hydrogel in peripheral nerve injury.
基金supported by European Union Funding Programme,PNRR,No. 760058(to DMH)the UEFISCDI Project,No. PN-III-P4-IDPCE-2020-059(to APW)
文摘The major aim of stroke therapy is to stimulate brain repair and improve behavioral recovery after cerebral ischemia.One option is to stimulate endogenous neurogenesis in the subventricular zone and direct the newly formed neurons to the damaged area.However,only a small percentage of these neurons survive,and many do not reach the damaged area,possibly because the corpus callosum impedes the migration of subventricular zone-derived stem cells into the lesioned cortex.A second major obstacle to stem cell therapy is the strong inflammatory reaction induced by cerebral ischemia,whereby the associated phagocytic activity of brain macrophages removes both therapeutic cells and/or cell-based drug carriers.To address these issues,neurogenesis was electrically stimulated in the subventricular zone,followed by isolation of proliferating cells,including newly formed neurons,which were subsequently mixed with a nutritional hydrogel.This mixture was then transferred to the stroke cavity of day 14 post-stroke mice.We found that the performance of the treated animals improved in behavioral tests,including novel object,open field,hole board,grooming,and“time-to-feel”adhesive tape tests.Furthermore,immunostaining revealed that the stem cell marker nestin,the neuroepithelial marker Mash1,and the immature neuronal marker doublecortin-positive cells survived in the transplanted area for 2 weeks,possibly due to reduced phagocytic activity and supportive angiogenesis.These results clearly indicate that the transplantation of committed subventricular zone stem cells combined with a protective nutritional gel directly into the infarct cavity after the peak of stroke-induced neuroinflammation represents a feasible approach to improve neurorestoration after cerebral ischemia.
