Among the 14 serovars of Listeria monocytogenes(Lm),serovar 4b strains are the most predominant isolates linked to human listeriosis outbreaks-a phenotype associated with their unique wall teichoic acid(WTA)decorated ...Among the 14 serovars of Listeria monocytogenes(Lm),serovar 4b strains are the most predominant isolates linked to human listeriosis outbreaks-a phenotype associated with their unique wall teichoic acid(WTA)decorated with galactose(Gal)and glucose(Glu).A wealth of knowledge is available for galactosylated-WTA(Gal-WTA)manipulating bacterial homeostasis and virulence,whereas the relationship between glucosylated-WTA(Glu-WTA)and Gal-WTA in listerial physiology and pathogenesis remains unclear.Here,we find that Glu-WTA and Gal-WTA jointly constitute the O-antigen pattern of serovar 4b Lm;however,Glu-WTA specifically serves as the indispensable ligand for listeriophage LP4 adsorption.Moreover,the co-operation between Glu-and Gal-WTA increases biofilm formation and bacterial resistance to cationic antimicrobial peptide(CRAMP).We further demonstrate that Gal-WTA modulates the anchoring of surface proteins,including IspC,Ami,and InlB.Additionally,dual glycosylated WTA interaction with ActA facilitates bacterial intracellular motility and dissemination.Consistently,Glu-WTA significantly enhances bacterial colonization ability in the mesenteric lymph nodes(MLNs),ileum,liver,and brain of mouse,cooperating with Gal-WTA to facilitate Lm dissemination to distant organs and tissues.In conclusion,we reveal the crucial roles of Glu-WTA in synergizing with Gal-WTA to modulate the integrity of the cell wall structure and exacerbate bacterial infection,providing a global understanding of the hypervirulence and pathogenicity of invasive serovar 4b Lm.展开更多
1 Results Several methods have been elaborated in this laboratory allowing preparation of macromolecules with phosphodiester bonds,and having sequence of atoms similar as in the chains of biomacromolecules - nucleic o...1 Results Several methods have been elaborated in this laboratory allowing preparation of macromolecules with phosphodiester bonds,and having sequence of atoms similar as in the chains of biomacromolecules - nucleic or teichoic acids (TA),namely:-(C)n-O-PO-,where n=2 (for teichoic acids) or 3.These methods,to be discussed in the lecture,are based on the ring-opening polymerization,transesterification,and recently elaborated direct addition of phosphoric acid to diepoxides.For the first time an attempt h...展开更多
Staphylococcus aureus is a major public health threat,largely due to its remarkable capacity to develop antimicro-bial resistance.Zhang et al.recently demonstrated a highly innovative approach to eradicate chronic met...Staphylococcus aureus is a major public health threat,largely due to its remarkable capacity to develop antimicro-bial resistance.Zhang et al.recently demonstrated a highly innovative approach to eradicate chronic methicillin-resistant S.aureus infections by inducing bacterial calcification with antibody-polysialic acid conjugates targeting wall teichoic acids,while simultaneously modulating host immune responses via enhanced calprotectin expres-sion and macrophage activation.Despite limitations,this strategy represents a promising and unconventional therapy to combat resistant S.aureus infections.展开更多
基金supported by the National Natural Science Foundation of China(32161133025 and 32373012)the Key Research and Development Program of Jiangsu(BE2021331)+2 种基金111 Project(D18007)the Priority Academic Development Program of Jiangsu Higher Education Institutions(PAPD),the State-funded Postdoctoral Researchers Program(GZC20241437)the Jiangsu Funding Program for Excellent Postdoctoral Talent(2024ZB641).
文摘Among the 14 serovars of Listeria monocytogenes(Lm),serovar 4b strains are the most predominant isolates linked to human listeriosis outbreaks-a phenotype associated with their unique wall teichoic acid(WTA)decorated with galactose(Gal)and glucose(Glu).A wealth of knowledge is available for galactosylated-WTA(Gal-WTA)manipulating bacterial homeostasis and virulence,whereas the relationship between glucosylated-WTA(Glu-WTA)and Gal-WTA in listerial physiology and pathogenesis remains unclear.Here,we find that Glu-WTA and Gal-WTA jointly constitute the O-antigen pattern of serovar 4b Lm;however,Glu-WTA specifically serves as the indispensable ligand for listeriophage LP4 adsorption.Moreover,the co-operation between Glu-and Gal-WTA increases biofilm formation and bacterial resistance to cationic antimicrobial peptide(CRAMP).We further demonstrate that Gal-WTA modulates the anchoring of surface proteins,including IspC,Ami,and InlB.Additionally,dual glycosylated WTA interaction with ActA facilitates bacterial intracellular motility and dissemination.Consistently,Glu-WTA significantly enhances bacterial colonization ability in the mesenteric lymph nodes(MLNs),ileum,liver,and brain of mouse,cooperating with Gal-WTA to facilitate Lm dissemination to distant organs and tissues.In conclusion,we reveal the crucial roles of Glu-WTA in synergizing with Gal-WTA to modulate the integrity of the cell wall structure and exacerbate bacterial infection,providing a global understanding of the hypervirulence and pathogenicity of invasive serovar 4b Lm.
文摘1 Results Several methods have been elaborated in this laboratory allowing preparation of macromolecules with phosphodiester bonds,and having sequence of atoms similar as in the chains of biomacromolecules - nucleic or teichoic acids (TA),namely:-(C)n-O-PO-,where n=2 (for teichoic acids) or 3.These methods,to be discussed in the lecture,are based on the ring-opening polymerization,transesterification,and recently elaborated direct addition of phosphoric acid to diepoxides.For the first time an attempt h...
基金financially supported by Independent Research Fund,Denmark(2035-00110B)the Novo Nordisk Foundation,Denmark(NNF22OC0077593)awarded to Hanne Ingmer.
文摘Staphylococcus aureus is a major public health threat,largely due to its remarkable capacity to develop antimicro-bial resistance.Zhang et al.recently demonstrated a highly innovative approach to eradicate chronic methicillin-resistant S.aureus infections by inducing bacterial calcification with antibody-polysialic acid conjugates targeting wall teichoic acids,while simultaneously modulating host immune responses via enhanced calprotectin expres-sion and macrophage activation.Despite limitations,this strategy represents a promising and unconventional therapy to combat resistant S.aureus infections.
