To the Editor:Radiation therapy(RT)is important in treating thoracic tumors.However,RT inevitably causes damage to normal lungs,leading to radiation-induced lung injury(RILI).Physical and biological factors conjointly...To the Editor:Radiation therapy(RT)is important in treating thoracic tumors.However,RT inevitably causes damage to normal lungs,leading to radiation-induced lung injury(RILI).Physical and biological factors conjointly determine RILI.Understanding the role of physical technology development in reducing RILI is more conducive to developing and applying radiotherapy physical technology.The physical technology mentioned here is mainly based on the principles and methods of physics(engineering)applied in RT.展开更多
Recently,the high incidence of oral mucosal defects and the subsequent functional impairments have attracted widespread attention.Controlling scaffold geometry pattern has been proposed as a strategy to promote cell b...Recently,the high incidence of oral mucosal defects and the subsequent functional impairments have attracted widespread attention.Controlling scaffold geometry pattern has been proposed as a strategy to promote cell behavior and facilitate soft tissue repair.In this study,we innovatively construct an integrated dual-layer heterogeneous polycaprolactone(PCL)scaffold using melt electrowriting(MEW)technology.The outer layer was disordered,while the inner layer featured oriented fiber patterns:parallel(P-par),rhombic(P-rhomb),and square(P-sq).Our findings revealed that the P-rhomb and P-sq scaffolds exhibited superior surface wettability,roughness,and tensile strength compared to the pure disordered PCL scaffolds(P)and P-par.Compared to the commercial collagen membranes,the outer layer of PCL can effectively inhibit bacterial adhesion and biofilm formation.Furthermore,the P-rhomb and P-sq groups demonstrated higher gene and protein expression levels related to cell adhesion and cell migration rates than did the P and P-par groups.Among them,P-sq plays an important role in inducing the differentiation of gingival fibroblasts into myofibroblasts rich inα-smooth muscle actin(α-SMA).Additionally,P-sq could reduce inflammation,promote epithelial regeneration,and accelerate wound healing when used in full-thickness oral mucosal defects in rabbits.Overall,the integrated dual-layer heterogeneous PCL scaffold fabricated by MEW technology effectively inhibited bacterial adhesion and guided tissue regeneration,offering advantages for clinical translation and large-scale production.This promising material holds important potential for treating full-thickness mucosal defects in a bacteria-rich oral environments.展开更多
Cell therapy is a promising strategy for acute liver failure(ALF),while its therapeutic efficacy is often limited by cell loss and poor arrangement.Here,inspired by liver microunits,we propose a novel spatially ordere...Cell therapy is a promising strategy for acute liver failure(ALF),while its therapeutic efficacy is often limited by cell loss and poor arrangement.Here,inspired by liver microunits,we propose a novel spatially ordered multicellular lobules for the ALF treatment by using a microfluidic continuous spinning technology.The microfluidics with multiple microchannels was constructed by assembling parallel capillaries.Sodium alginate(Alg)solution encapsulating human umbilical vein endothelial cells(HUVECs),hepatocytes,and mesenchymal stem cells(MSCs)are introduced into the middle channel and the 6 parallel outer channels of the microfluidics,respectively.Simultaneously,Ca^(2+)-loaded solutions are pumped through the innermost and outermost channels,forming a hollow microfiber with hepatocytes and MSCs alternately surrounding the HUVECs.These microfibers could highly resemble the cord-like structure of liver lobules,bringing about outstanding liver-like functions.We have demonstrated that in ALF rats,our biomimetic lobules can effectively suppress excessive inflammatory responses,decrease cell necrosis,and promote regenerative pathways,leading to satisfied therapeutic efficacy.These findings underscore the potential of spatially ordered multicellular microfibers in treating related diseases and improving traditional clinical methods.展开更多
基金Project of Science,Technology Department of Yunnan Province and Kunming Medical University(No.202101AY070001-164)
文摘To the Editor:Radiation therapy(RT)is important in treating thoracic tumors.However,RT inevitably causes damage to normal lungs,leading to radiation-induced lung injury(RILI).Physical and biological factors conjointly determine RILI.Understanding the role of physical technology development in reducing RILI is more conducive to developing and applying radiotherapy physical technology.The physical technology mentioned here is mainly based on the principles and methods of physics(engineering)applied in RT.
基金funded by the Key Research and Development Program of Zhejiang Province(2021C03059 and 2024C03081)the National Natural Science Foundation of China(81801024)the Research and Development Project of Stomatology Hospital Zhejiang University School of Medicine(RD2023JXXK01).
文摘Recently,the high incidence of oral mucosal defects and the subsequent functional impairments have attracted widespread attention.Controlling scaffold geometry pattern has been proposed as a strategy to promote cell behavior and facilitate soft tissue repair.In this study,we innovatively construct an integrated dual-layer heterogeneous polycaprolactone(PCL)scaffold using melt electrowriting(MEW)technology.The outer layer was disordered,while the inner layer featured oriented fiber patterns:parallel(P-par),rhombic(P-rhomb),and square(P-sq).Our findings revealed that the P-rhomb and P-sq scaffolds exhibited superior surface wettability,roughness,and tensile strength compared to the pure disordered PCL scaffolds(P)and P-par.Compared to the commercial collagen membranes,the outer layer of PCL can effectively inhibit bacterial adhesion and biofilm formation.Furthermore,the P-rhomb and P-sq groups demonstrated higher gene and protein expression levels related to cell adhesion and cell migration rates than did the P and P-par groups.Among them,P-sq plays an important role in inducing the differentiation of gingival fibroblasts into myofibroblasts rich inα-smooth muscle actin(α-SMA).Additionally,P-sq could reduce inflammation,promote epithelial regeneration,and accelerate wound healing when used in full-thickness oral mucosal defects in rabbits.Overall,the integrated dual-layer heterogeneous PCL scaffold fabricated by MEW technology effectively inhibited bacterial adhesion and guided tissue regeneration,offering advantages for clinical translation and large-scale production.This promising material holds important potential for treating full-thickness mucosal defects in a bacteria-rich oral environments.
基金supported by the National Natural Science Foundation of China(82270646,T2225003,and 82100664)the National Key Research and Development Program of China(2022YFA1105300)+5 种基金the Jiangsu Provincial Science and Technology Special Fund for Outstanding Young Scholars(BK20230051)Special Funding of Jiangsu Province Concept Validation Center Nanjing Drum Tower Hospital(2024-GNYZ-ZD-03)the Nanjing Health Science and Technology Development Project for Distinguished Young Scholars(JQX22003)the Clinical Trials from Nanjing Drum Tower Hospital(2022-LCYJ-ZD-01)the Guangdong Basic and Applied Basic Research Foundation(2021B1515120054)the Joint Fund of Henan Province Science and Technology R&D Program(Project No.225200810021).
文摘Cell therapy is a promising strategy for acute liver failure(ALF),while its therapeutic efficacy is often limited by cell loss and poor arrangement.Here,inspired by liver microunits,we propose a novel spatially ordered multicellular lobules for the ALF treatment by using a microfluidic continuous spinning technology.The microfluidics with multiple microchannels was constructed by assembling parallel capillaries.Sodium alginate(Alg)solution encapsulating human umbilical vein endothelial cells(HUVECs),hepatocytes,and mesenchymal stem cells(MSCs)are introduced into the middle channel and the 6 parallel outer channels of the microfluidics,respectively.Simultaneously,Ca^(2+)-loaded solutions are pumped through the innermost and outermost channels,forming a hollow microfiber with hepatocytes and MSCs alternately surrounding the HUVECs.These microfibers could highly resemble the cord-like structure of liver lobules,bringing about outstanding liver-like functions.We have demonstrated that in ALF rats,our biomimetic lobules can effectively suppress excessive inflammatory responses,decrease cell necrosis,and promote regenerative pathways,leading to satisfied therapeutic efficacy.These findings underscore the potential of spatially ordered multicellular microfibers in treating related diseases and improving traditional clinical methods.
