In this paper, we demonstrated a one-step template-free strategy to fabricate a hollow mesoporous structured NaY F4：Yb,Er nanoparticles with excellent upconversion luminescence. Folic acid（FA）, a commonly used canc...In this paper, we demonstrated a one-step template-free strategy to fabricate a hollow mesoporous structured NaY F4：Yb,Er nanoparticles with excellent upconversion luminescence. Folic acid（FA）, a commonly used cancer-targeting agent, was conjugated on the surface of the nanoparticles based on the presence of free amine groups, which were labeled as NaY F4：Yb,Er-FA HMUCNPs. The properties were extensively studied, which indicated the obtained samples showed a typical hollow mesoporous structure and excellent upconversion luminescence that were useful for cell imaging and drug delivery. The L929 cells viability, hemolysis assay and coagulation test demonstrated good biocompatibility of the samples. The anti-cancer drug doxorubicin hydrochloride（DOX） storage/release properties were demonstrated to be pH-responsive, in which, the drug release might be beneficial at the reduced pH for targeted release and controlled therapy. Moreover, it was found that DOX-loaded NaY F4：Yb,Er-FA HMUCNPs exhibited greater cytotoxicity to KB cells than free DOX due to the specific cell uptake by KB cells via folate receptor-mediate endocytosis. Therefore, the multifunctional nanoparticles combining upconversion luminescent property and hollow mesoporous structure have potential for simultaneous targeted anti-cancer drug delivery and cell imaging.展开更多
Intercellular communication between neurons and glial cells via extracellular vesicles（EVs）as a novel mechanism of information transfer has been shown to be involved in regeneration processes within the central nerv...Intercellular communication between neurons and glial cells via extracellular vesicles（EVs）as a novel mechanism of information transfer has been shown to be involved in regeneration processes within the central nervous system（CNS）（Rajendran et al.,2014）.Hence,to take advantage of EV signaling for therapeutic applications appears to be a completely new approach to promote regeneration.展开更多
Polyurethane micelles（PM）-based nanovehicles have shown great potential in targeted delivery of therapeutics and diagnostics into tumors.However,the pathways of PMs entering cancer cells and the action mechanism of ...Polyurethane micelles（PM）-based nanovehicles have shown great potential in targeted delivery of therapeutics and diagnostics into tumors.However,the pathways of PMs entering cancer cells and the action mechanism of targeting ligands have yet to be understood.In this contribution,the actively-targeted PM were developed using trastuzumab as a model targeting group.It was found that PM were mainly taken up by SKOV-3 tumor cells via a micropinocytosis process,while the incorporation of trastuzumab to PM enabled a receptor-mediated endocytosis of nanocarriers in cancer cells,leading to more efficient cell entry and enhanced anticancer efficacy of chemotherapeutic drugs both in vitro and in vivo.This study is advantageous to the understanding of the action mechanism of trastuzumab,and significant for the construction of improved formulations for targeted delivery and precise therapy.展开更多
基金Project supported by the National Natural Science Foundation of China(51372201)the Specialized Research Fund of Education Department of Shaanxi Province(16JK1242)+1 种基金Project of Science and Technology Special of Shangluo(SK2015-36)the Scientific Research Foundation of Shangluo University(15SKY021)
文摘In this paper, we demonstrated a one-step template-free strategy to fabricate a hollow mesoporous structured NaY F4：Yb,Er nanoparticles with excellent upconversion luminescence. Folic acid（FA）, a commonly used cancer-targeting agent, was conjugated on the surface of the nanoparticles based on the presence of free amine groups, which were labeled as NaY F4：Yb,Er-FA HMUCNPs. The properties were extensively studied, which indicated the obtained samples showed a typical hollow mesoporous structure and excellent upconversion luminescence that were useful for cell imaging and drug delivery. The L929 cells viability, hemolysis assay and coagulation test demonstrated good biocompatibility of the samples. The anti-cancer drug doxorubicin hydrochloride（DOX） storage/release properties were demonstrated to be pH-responsive, in which, the drug release might be beneficial at the reduced pH for targeted release and controlled therapy. Moreover, it was found that DOX-loaded NaY F4：Yb,Er-FA HMUCNPs exhibited greater cytotoxicity to KB cells than free DOX due to the specific cell uptake by KB cells via folate receptor-mediate endocytosis. Therefore, the multifunctional nanoparticles combining upconversion luminescent property and hollow mesoporous structure have potential for simultaneous targeted anti-cancer drug delivery and cell imaging.
文摘Intercellular communication between neurons and glial cells via extracellular vesicles（EVs）as a novel mechanism of information transfer has been shown to be involved in regeneration processes within the central nervous system（CNS）（Rajendran et al.,2014）.Hence,to take advantage of EV signaling for therapeutic applications appears to be a completely new approach to promote regeneration.
基金financially supported by the National Natural Science Foundation of China(Nos.51273126 and 51573112)the National Science Fund for Distinguished Young Scholars of China(No.51425305)the Youth Science and Technology Innovation Team of Sichuan Province(No.2015TD0001)
文摘Polyurethane micelles（PM）-based nanovehicles have shown great potential in targeted delivery of therapeutics and diagnostics into tumors.However,the pathways of PMs entering cancer cells and the action mechanism of targeting ligands have yet to be understood.In this contribution,the actively-targeted PM were developed using trastuzumab as a model targeting group.It was found that PM were mainly taken up by SKOV-3 tumor cells via a micropinocytosis process,while the incorporation of trastuzumab to PM enabled a receptor-mediated endocytosis of nanocarriers in cancer cells,leading to more efficient cell entry and enhanced anticancer efficacy of chemotherapeutic drugs both in vitro and in vivo.This study is advantageous to the understanding of the action mechanism of trastuzumab,and significant for the construction of improved formulations for targeted delivery and precise therapy.
