Spinal cord injury（SCI）frequently results in the permanent loss of function below the level of injury due to the failure of axonal regeneration in the adult mammalian central nervous system（CNS）.The limited intrin...Spinal cord injury（SCI）frequently results in the permanent loss of function below the level of injury due to the failure of axonal regeneration in the adult mammalian central nervous system（CNS）.The limited intrinsic growth capacity of adult neurons,a lack of growth-promoting factors and the multifactorial inhibitory microenvironment around the lesion site contribute to the lack of axonalregeneration. Strategies such as transplantation of cells,展开更多
Precise genome modification with engineered nucleases is a powerful tool for studying basic biology and applied biotechnology. Transcription activator-like effector nucleases（TALENs）,consisting of an engineered spec...Precise genome modification with engineered nucleases is a powerful tool for studying basic biology and applied biotechnology. Transcription activator-like effector nucleases（TALENs）,consisting of an engineered specific（TALE） DNA binding domain and a Fok I cleavage domain,are newly developed versatile reagents for genome engineering in different organisms.Because of the simplicity of the DNA recognition code and their modular assembly,TALENs can act as customizable molecular DNA scissors inducing double-strand breaks（DSBs） at given genomic location.Thus,they provide a valuable approach to targeted genome modifications such as mutations, insertions,replacements or chromosome rearrangements.In this article,we review the development of TALENs,and summarize the principles and tools for TALEN-mediated gene targeting in plant cells,as well as current and potential strategies for use in plant research and crop improvement.展开更多
With the developments in metabolic engineering and the emergence of synthetic biology,many breakthroughs in medicinal,biological and chemical products as well as biofuels have been achieved in recent decades.As an imp...With the developments in metabolic engineering and the emergence of synthetic biology,many breakthroughs in medicinal,biological and chemical products as well as biofuels have been achieved in recent decades.As an important barrier to traditional metabolic engineering,however,the identification of ratelimiting step(s)for the improvement of specific cellular functions is often difficult.Meanwhile,in the case of synthetic biology,more and more BioBricks could be constructed for targeted purposes,but the optimized assembly or engineering of these components for high-efficiency cell factories is still a challenge.Owing to the lack of steady-state kinetic data for overall flux,balancing many multistep biosynthetic pathways is time-consuming and needs vast resources of labor and materials.A strategy called targeted engineering is proposed in an effort to solve this problem.Briefly,a targeted biosynthetic pathway is to be reconstituted in vitro and then the contribution of cofactors,substrates and each enzyme will be analyzed systematically.Next is in vivo engineering or de novo pathway assembly with the guidance of information gained from in vitro assays.To demonstrate its practical application,biosynthesis pathways for the production of important products,e.g.chemicals,nutraceuticals and drug precursors,have been engineered in Escherichia coli and Saccharomyces cerevisiae.These cases can be regarded as concept proofs indicating targeted engineering might help to create high-efficiency cell factories based upon constructed biological components.展开更多
The goals of engineering and scientific missions for Chang＇E-2 lunar satellite require high detection sensitivity and large imaging dynamic range for the onboard CCD cameras. The TDI CCD image sensor was adopted for ...The goals of engineering and scientific missions for Chang＇E-2 lunar satellite require high detection sensitivity and large imaging dynamic range for the onboard CCD cameras. The TDI CCD image sensor was adopted for the two linear CCD stereo cameras for the first time in the lunar reconnaissance of the world. The design argumentation is described in this paper. The analysis shows that the imagers meet the mission requirements. The satellite was launched on 1 October 2010 at zero window. The cameras obtained images of 7 m resolution on the 100 km orbit for the first time on 24 October 2010, and operated once again on 27 October 2010 to take stereo images of the Sinus Iridum with the resolution better than 1.5 m. On the near-moon-arc of 15 kmxl00 km elliptical orbit, the images are very clear and rich of grey scales, indicating successful completion of the Chang＇E-2 engineering mission. At the present the cameras are acquiring the full lunar surface stereo images with 7 m resolution on the 100 km circular orbit to complete their scientific mission.展开更多
基金Supported by grants from the Deutsche Forschungsgemeinschaft(BL414/3-1)International Foundation for Research in Paraplegia+2 种基金the Indiana University Health-Indiana University School of Medicine Strategic Research InitiativeIndiana Spinal Cord and Brain Injury Research FundMorton Cure Paralysis Fund to AB and a Heinz Gotze Memorial Fellowship to SL
文摘Spinal cord injury（SCI）frequently results in the permanent loss of function below the level of injury due to the failure of axonal regeneration in the adult mammalian central nervous system（CNS）.The limited intrinsic growth capacity of adult neurons,a lack of growth-promoting factors and the multifactorial inhibitory microenvironment around the lesion site contribute to the lack of axonalregeneration. Strategies such as transplantation of cells,
基金supported by the National Natural Science Foundation of China(Grant Nos.201263,383601 and 31200273)
文摘Precise genome modification with engineered nucleases is a powerful tool for studying basic biology and applied biotechnology. Transcription activator-like effector nucleases（TALENs）,consisting of an engineered specific（TALE） DNA binding domain and a Fok I cleavage domain,are newly developed versatile reagents for genome engineering in different organisms.Because of the simplicity of the DNA recognition code and their modular assembly,TALENs can act as customizable molecular DNA scissors inducing double-strand breaks（DSBs） at given genomic location.Thus,they provide a valuable approach to targeted genome modifications such as mutations, insertions,replacements or chromosome rearrangements.In this article,we review the development of TALENs,and summarize the principles and tools for TALEN-mediated gene targeting in plant cells,as well as current and potential strategies for use in plant research and crop improvement.
基金the National 973 Program of China(No.2011CBA00800 and 2012CB721000)National 863 Program of China(No.2012AA02A701)+2 种基金National Natural Science Foundation of China(No.31170096,312220170 and 31500072)the Natural Science Foundation of Hubei Province(No.2015CFB415)China Postdoctoral Science Foundation Grant(No.2014M562052).
文摘With the developments in metabolic engineering and the emergence of synthetic biology,many breakthroughs in medicinal,biological and chemical products as well as biofuels have been achieved in recent decades.As an important barrier to traditional metabolic engineering,however,the identification of ratelimiting step(s)for the improvement of specific cellular functions is often difficult.Meanwhile,in the case of synthetic biology,more and more BioBricks could be constructed for targeted purposes,but the optimized assembly or engineering of these components for high-efficiency cell factories is still a challenge.Owing to the lack of steady-state kinetic data for overall flux,balancing many multistep biosynthetic pathways is time-consuming and needs vast resources of labor and materials.A strategy called targeted engineering is proposed in an effort to solve this problem.Briefly,a targeted biosynthetic pathway is to be reconstituted in vitro and then the contribution of cofactors,substrates and each enzyme will be analyzed systematically.Next is in vivo engineering or de novo pathway assembly with the guidance of information gained from in vitro assays.To demonstrate its practical application,biosynthesis pathways for the production of important products,e.g.chemicals,nutraceuticals and drug precursors,have been engineered in Escherichia coli and Saccharomyces cerevisiae.These cases can be regarded as concept proofs indicating targeted engineering might help to create high-efficiency cell factories based upon constructed biological components.
文摘The goals of engineering and scientific missions for Chang＇E-2 lunar satellite require high detection sensitivity and large imaging dynamic range for the onboard CCD cameras. The TDI CCD image sensor was adopted for the two linear CCD stereo cameras for the first time in the lunar reconnaissance of the world. The design argumentation is described in this paper. The analysis shows that the imagers meet the mission requirements. The satellite was launched on 1 October 2010 at zero window. The cameras obtained images of 7 m resolution on the 100 km orbit for the first time on 24 October 2010, and operated once again on 27 October 2010 to take stereo images of the Sinus Iridum with the resolution better than 1.5 m. On the near-moon-arc of 15 kmxl00 km elliptical orbit, the images are very clear and rich of grey scales, indicating successful completion of the Chang＇E-2 engineering mission. At the present the cameras are acquiring the full lunar surface stereo images with 7 m resolution on the 100 km circular orbit to complete their scientific mission.
