Phosphorene,which is designated as a monolayer or a few-layer of orthorhombic black phosphorus(BP),has emerged as a new promising member of the two-dimensional material family.A plethora of exciting applications of ph...Phosphorene,which is designated as a monolayer or a few-layer of orthorhombic black phosphorus(BP),has emerged as a new promising member of the two-dimensional material family.A plethora of exciting applications of phosphorene have been reported based on its intriguing properties.Unfortunately,due to the lack of reliable methods for the direct synthesis of highly crystalline phosphorene,bulk BP single crys tals have been the sole precursor to achieve high-quality phosphorene.A chemical vapor transport(CVT)reaction method has been deemed the most successful method for the growth of bulk BP single crystals.Herein,recent progress in the controlled growth of bulk BP single crystals by the CVT reaction is briefly reviewed.The emphasis is focused on the reaction system,nucleation and growth mechanism of the CVT synthesis of bulk BP crystals as well as the recent development in the growth of doped and substituted BP single crystals by the CVT reaction method.The challenges and perspectives ahead of the CVT growth of BP crystals are also pointed out.展开更多
Background:To investigate SCL/TAL 1 interrupting locus(STIL)’s role and prognostic significance in lung adenocarcinoma(LUAD)progression,we examined STIL and E2 promoter binding factor 1(E2F1)expression and their impa...Background:To investigate SCL/TAL 1 interrupting locus(STIL)’s role and prognostic significance in lung adenocarcinoma(LUAD)progression,we examined STIL and E2 promoter binding factor 1(E2F1)expression and their impacts on LUAD prognosis using Gene Expression Profiling Interactive Analysis(GEPIA).Methods:Functional assays including CCK-8,wound-healing,5-ethynyl-2-deoxyuridine(EdU),Transwell assays,and flow cytometry,elucidated STIL and E2F1’s effects on cell viability,proliferation,apoptosis,and migration.Gene set enrichment analysis(GSEA)identified potential pathways,while metabolic assays assessed glucose metabolism.Results:Our findings reveal that STIL and E2F1 are overexpressed in LUAD,correlating with adverse outcomes.It enhances cell proliferation,migration,and invasion,and suppresses apoptosis,activating downstream of E2F1.Silencing E2F1 reversed the promotion effect of the STIL overexpression on cell viability and invasiveness.Importantly,STIL modulates glycolysis,influencing glucose consumption,lactate production,and energy balance in LUAD cells.Conclusion:Our model,incorporating STIL,age,and disease stage,robustly predicts patient prognosis,underscored STIL’s pivotal role in LUAD pathogenesis through metabolic reprogramming.This comprehensive approach not only confirms STIL’s prognostic value but also highlights its potential as a therapeutic target in LUAD.展开更多
基金supported by the National Natural Science Foundation of China(No.21671115)the Science Challenge Project(TZ2018004)+1 种基金the Innovation Center of Radiation Application(KFZC2018040204)the Tsinghua University Initiative Scientific Research Program(2018Z05JZY022).
文摘Phosphorene,which is designated as a monolayer or a few-layer of orthorhombic black phosphorus(BP),has emerged as a new promising member of the two-dimensional material family.A plethora of exciting applications of phosphorene have been reported based on its intriguing properties.Unfortunately,due to the lack of reliable methods for the direct synthesis of highly crystalline phosphorene,bulk BP single crys tals have been the sole precursor to achieve high-quality phosphorene.A chemical vapor transport(CVT)reaction method has been deemed the most successful method for the growth of bulk BP single crystals.Herein,recent progress in the controlled growth of bulk BP single crystals by the CVT reaction is briefly reviewed.The emphasis is focused on the reaction system,nucleation and growth mechanism of the CVT synthesis of bulk BP crystals as well as the recent development in the growth of doped and substituted BP single crystals by the CVT reaction method.The challenges and perspectives ahead of the CVT growth of BP crystals are also pointed out.
文摘Background:To investigate SCL/TAL 1 interrupting locus(STIL)’s role and prognostic significance in lung adenocarcinoma(LUAD)progression,we examined STIL and E2 promoter binding factor 1(E2F1)expression and their impacts on LUAD prognosis using Gene Expression Profiling Interactive Analysis(GEPIA).Methods:Functional assays including CCK-8,wound-healing,5-ethynyl-2-deoxyuridine(EdU),Transwell assays,and flow cytometry,elucidated STIL and E2F1’s effects on cell viability,proliferation,apoptosis,and migration.Gene set enrichment analysis(GSEA)identified potential pathways,while metabolic assays assessed glucose metabolism.Results:Our findings reveal that STIL and E2F1 are overexpressed in LUAD,correlating with adverse outcomes.It enhances cell proliferation,migration,and invasion,and suppresses apoptosis,activating downstream of E2F1.Silencing E2F1 reversed the promotion effect of the STIL overexpression on cell viability and invasiveness.Importantly,STIL modulates glycolysis,influencing glucose consumption,lactate production,and energy balance in LUAD cells.Conclusion:Our model,incorporating STIL,age,and disease stage,robustly predicts patient prognosis,underscored STIL’s pivotal role in LUAD pathogenesis through metabolic reprogramming.This comprehensive approach not only confirms STIL’s prognostic value but also highlights its potential as a therapeutic target in LUAD.
