Consistent with previous research, results showed men get more paragraphs and photography coverage. Interestingly, women's factual information is always associated with relevant men report, including P.E relevant ...Consistent with previous research, results showed men get more paragraphs and photography coverage. Interestingly, women's factual information is always associated with relevant men report, including P.E relevant with personal information.展开更多
The regional policy in the innovation development of private P.E economy plays better role in management according to law,guidance and escort;increase the macro quality of main body of private P.E entrepreneurs and th...The regional policy in the innovation development of private P.E economy plays better role in management according to law,guidance and escort;increase the macro quality of main body of private P.E entrepreneurs and the overall staff quality,develop P.E vocational P.E to provide P.E talents for private P.E economy.展开更多
In this paper, the physical education information teaching design system is researched and developed based on VB6.0 optimization platform. According to the national physical education and health course standards and t...In this paper, the physical education information teaching design system is researched and developed based on VB6.0 optimization platform. According to the national physical education and health course standards and the fundamental theories of the constructivisrn, the author adopts the modular-design development method to design two functional areas which are the displaying of the teaching design contents and the online information database （nine modules in total） on the basis of the P.E. teaching design flow. Importantly, this system features the compatibility, scalability and practical applicability, and can effectively improve the ability of P.E. teachers to design their teaching, and hence provides a guarantee for the improvement of teaching quality and efficiency.展开更多
In the new period, the physical education in colleges and universities is of greater and greater significance for the enhancement of students＇ learning efficiency. In this paper, the current situation of the current ...In the new period, the physical education in colleges and universities is of greater and greater significance for the enhancement of students＇ learning efficiency. In this paper, the current situation of the current physical education is analyzed, and also related suggestions on the optimization of the P.E. course teaching plan are proposed.展开更多
With the development of the times and the reform of the education system, the state pays more attention to vocational education and the training of technical personnel, and the society's demand for talents in vari...With the development of the times and the reform of the education system, the state pays more attention to vocational education and the training of technical personnel, and the society's demand for talents in various industries is constantly improving and refined. "Morality cultivation" is the basic principle of education in higher vocational colleges. It is not conducive to the all-round development of students and does not meet the requirements of training builders and successors of socialism with Chinese characteristics to lay particular stress on scientific research results, skills competition awards, employment rate of graduates and other indicators, while neglecting the cultivation of students' "morality". It is particularly important to create a good academic atmosphere on campus, to promote the educational concept of "cultivating morality and cultivating people" in the process of higher vocational colleges' education management, to enable students to improve their own code of conduct, ideological and moral character, to set up lofty aspirations and life goals, and to cultivate the correct outlook on life and values of contemporary college students in a strong learning atmosphere.展开更多
This article comments on the study by Zhang et al,which proposed that exosomes derived from hypoxia-injured endometrial epithelial cells promote human umbilical cord mesenchymal stem cell migration and differentiation...This article comments on the study by Zhang et al,which proposed that exosomes derived from hypoxia-injured endometrial epithelial cells promote human umbilical cord mesenchymal stem cell migration and differentiation into endo-metrial epithelial cells via exosomal miR-137-3p.The authors demonstrated that miR-137-3p targets ubiquitin protein ligase E3C and activates signal transducer and activator of transcription 3 signaling,thereby driving epithelial lineage transition.While this study expands our understanding of exosome-mediated intercellular communication in endometrial repair,several key gaps remain.Notably,microRNA(miRNA)profiling was performed in human umbilical cord mesenchymal stem cells post-exosome treatment,not in the exosomes derived from hypoxia-injured endometrial epithelial cell themselves,leaving open whether miR-137-3p is directly transferred or indirectly induced.In addition,data on exosome characterization were unavailable,and the rationale for selecting miR-137-3p over other differentially expressed miRNAs was not well justified.Future studies should include direct exosomal miRNA content analysis,in vivo validation,and deeper mechanistic exploration of the ubiquitin protein ligase E3C-signal transducer and activator of transcription 3 ubiquitination axis to establish the clinical and biological relevance of this pathway.展开更多
BACKGROUND Thin endometrium,leading cause of recurrent implantation failure and infertility,has been found to respond to exosomes.AIM To investigate the efficacy of exosomes in addressing the issue of thin endometrium...BACKGROUND Thin endometrium,leading cause of recurrent implantation failure and infertility,has been found to respond to exosomes.AIM To investigate the efficacy of exosomes in addressing the issue of thin endometrium.METHODS RNA sequencing and reverse transcription-quantitative polymerase chain reaction were employed to identify differentially expressed microRNAs(miRNAs)in human umbilical cord mesenchymal stem cell(hucMSC)treated with exosomes enriched with endometrial cell-derived components.Additionally,Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were conducted to highlight significant enrichment in specific biological pathways,molecular functions,and cellular components.Transwell and wound healing assays were performed to assess migratory potential,and western blotting was detected protein level.RESULTS A total of 53 differentially expressed miRNAs were identified in hucMSC treated with exosomes enriched with endometrial cell-derived components,comprising 27 upregulated and 26 downregulated miRNAs,which includes miR-137-3p.Enhanced migratory potential was observed in the Transwell and wound healing assays,and western blotting confirmed the epithelial differentiation of hucMSC and the increased p-signal transducer and activator of transcription 3.These effects were attributed to the upregulation of miR-137-3p.CONCLUSION miR-137-3p in exosomes from hypoxia-affected endometrial epithelial cell stimulates the signal transducer and activator of transcription 3 signaling pathway,enhancing the migration and differentiation of hucMSC into endometrial epithelial cell.展开更多
This study by Zhang et al elucidates the role of exosome miR-137-3p targeting ubiquitin protein ligase E3C to activate signal transducer and activator of transcription 3 under hypoxia conditions,thereby promoting the ...This study by Zhang et al elucidates the role of exosome miR-137-3p targeting ubiquitin protein ligase E3C to activate signal transducer and activator of transcription 3 under hypoxia conditions,thereby promoting the migration and differentiation of human umbilical cord mesenchymal stem cells to endometrial epithelial cells.It emphasizes that exosomal miR-137-3p/ubiquitin protein ligase E3C/signal transducer and activator of transcription 3 axis is a promising pathway for endometrial regeneration.This article introduced the therapeutic potential of exosomal microRNAs in regenerative medicine while underscoring the need for standardized protocols in optimizing exosome delivery and validating molecular pathways for clinical translation.展开更多
A family of high_order accuracy explicit difference schemes for solving 2_dimension parabolic P.D.E. are constructed. Th e stability condition is r=Δt/Δx 2=Δt/Δy 2【1/2 and the truncation err or is O(Δt 3+Δx...A family of high_order accuracy explicit difference schemes for solving 2_dimension parabolic P.D.E. are constructed. Th e stability condition is r=Δt/Δx 2=Δt/Δy 2【1/2 and the truncation err or is O(Δt 3+Δx 4).展开更多
Two new freshwater atyid shrimps, Caridina angustipes sp nov.and C clavipes sp nov.from Hunan Province,China are described. C angustipes sp nov.is similar to C baojingensis Guo,He et Bai,1992,b...Two new freshwater atyid shrimps, Caridina angustipes sp nov.and C clavipes sp nov.from Hunan Province,China are described. C angustipes sp nov.is similar to C baojingensis Guo,He et Bai,1992,but with longer rostrum,15-27 dorsal teeth,3-6 ventral teeth,and straighter inner margin of endopods in male 1st pleopods. C clavipes sp nov.is similar to C angustipes ,but with narrower distal and fewer spines on inner margin of endopds in male 1st pleopods,and shorter and thinner appendix masculina in male 2nd pleopods with only 1 spine on inner lateral margin and 4 spines in apex.The holotypes are deposited in Shanghai Fisheries University.Some representative paratypes are deposited in Foshan Science and Technology College.展开更多
The whole plant with root ofHedyotis diffusa Willd. （AE） has been demonstrated to have anti-inflammatory, antioxidant, and anti-bacterial activities. In our study, we aim to examine the anti-tumor effect of alcoholi...The whole plant with root ofHedyotis diffusa Willd. （AE） has been demonstrated to have anti-inflammatory, antioxidant, and anti-bacterial activities. In our study, we aim to examine the anti-tumor effect of alcoholic extract of AE in mice implanted with sarcoma S180 cells （SBT mice）. We also compared the immune system function and the life span of SBT mice with that of control mice. We found that AE displayed a significant inhibitory effect on solid tumor growth, as well as increased the life span. At the dose of 10 mg/kg body weight, both tumor weight and volume were decreased significantly. We also measured several immune fimction markers, inlcuding spleen index （SI）, thymus index （TI） and parameters of hematological. We observed no reduce in these markers, indicatine that AE could inhibit tumor ~rowth without affectino immune function in SBT ,nice.展开更多
Objective: To study the prognostic value of the pathological margin and molecular margin marked by eIF4E and P53 protein in laryngeal carcinoma. Methods: The prognostic value of pathological and molecular margin was s...Objective: To study the prognostic value of the pathological margin and molecular margin marked by eIF4E and P53 protein in laryngeal carcinoma. Methods: The prognostic value of pathological and molecular margin was studied in 253 cases and 67 cases respectively, the latter were pathological negative margin chosen from the former. Immunohistochemisty was used to detect the expression of eIF4E and p53 proteins. Results: The rate of pathological, p53 and eIF4E positive margins was 20.2%, 19.4% and 32.8% respectively. The recurrent rate of those with positive margins was higher than that of negative margins, which including pathological margin (70.6% vs 35.1%, P =0.0000), p53 margin (69.2% vs 33.3%, P =0.018) and eIF4E margin (63.6% vs 28.9%, P =0.018); The survival rate of those with negative margins was higher than those with positive margins, including pathological margin (the 5-year cumulative survival rate was 37.52% and 64.37% respectively, P =0.0023), p53 margin (the 5-year cumulative survival rate was 24.62% and 75.69% respectively, P =0.0012) and eIF4E margin (the 5-year cumulative survival rate was 43.31% and 77.52% respectively, P =0.0006). Conclusion: The prognosis of those with both pathological and molecular positive margins was worse than that of the negative margins; Both the eIF4E and p53 were useful markers to pick out the poor prognostic patients from those with pathological negative margin, and the former seemed to be more potential.展开更多
The portal is characterized with large amount of information, high stability and interaction, while "Online Journal of Physical P.E" is to publicize the academic pure value, spread the achievement influence ...The portal is characterized with large amount of information, high stability and interaction, while "Online Journal of Physical P.E" is to publicize the academic pure value, spread the achievement influence in daily life, and advocate an academic atmosphere of utilitarianism with a slogan of "article issued online can be published in journal with the recommendation of experts" and practical action. It is the first academic journal under network operation.展开更多
文摘Consistent with previous research, results showed men get more paragraphs and photography coverage. Interestingly, women's factual information is always associated with relevant men report, including P.E relevant with personal information.
文摘The regional policy in the innovation development of private P.E economy plays better role in management according to law,guidance and escort;increase the macro quality of main body of private P.E entrepreneurs and the overall staff quality,develop P.E vocational P.E to provide P.E talents for private P.E economy.
文摘In this paper, the physical education information teaching design system is researched and developed based on VB6.0 optimization platform. According to the national physical education and health course standards and the fundamental theories of the constructivisrn, the author adopts the modular-design development method to design two functional areas which are the displaying of the teaching design contents and the online information database （nine modules in total） on the basis of the P.E. teaching design flow. Importantly, this system features the compatibility, scalability and practical applicability, and can effectively improve the ability of P.E. teachers to design their teaching, and hence provides a guarantee for the improvement of teaching quality and efficiency.
文摘In the new period, the physical education in colleges and universities is of greater and greater significance for the enhancement of students＇ learning efficiency. In this paper, the current situation of the current physical education is analyzed, and also related suggestions on the optimization of the P.E. course teaching plan are proposed.
文摘With the development of the times and the reform of the education system, the state pays more attention to vocational education and the training of technical personnel, and the society's demand for talents in various industries is constantly improving and refined. "Morality cultivation" is the basic principle of education in higher vocational colleges. It is not conducive to the all-round development of students and does not meet the requirements of training builders and successors of socialism with Chinese characteristics to lay particular stress on scientific research results, skills competition awards, employment rate of graduates and other indicators, while neglecting the cultivation of students' "morality". It is particularly important to create a good academic atmosphere on campus, to promote the educational concept of "cultivating morality and cultivating people" in the process of higher vocational colleges' education management, to enable students to improve their own code of conduct, ideological and moral character, to set up lofty aspirations and life goals, and to cultivate the correct outlook on life and values of contemporary college students in a strong learning atmosphere.
基金Supported by the General Program of the Natural Science Foundation of Guangdong Province,No.2025A1515011163.
文摘This article comments on the study by Zhang et al,which proposed that exosomes derived from hypoxia-injured endometrial epithelial cells promote human umbilical cord mesenchymal stem cell migration and differentiation into endo-metrial epithelial cells via exosomal miR-137-3p.The authors demonstrated that miR-137-3p targets ubiquitin protein ligase E3C and activates signal transducer and activator of transcription 3 signaling,thereby driving epithelial lineage transition.While this study expands our understanding of exosome-mediated intercellular communication in endometrial repair,several key gaps remain.Notably,microRNA(miRNA)profiling was performed in human umbilical cord mesenchymal stem cells post-exosome treatment,not in the exosomes derived from hypoxia-injured endometrial epithelial cell themselves,leaving open whether miR-137-3p is directly transferred or indirectly induced.In addition,data on exosome characterization were unavailable,and the rationale for selecting miR-137-3p over other differentially expressed miRNAs was not well justified.Future studies should include direct exosomal miRNA content analysis,in vivo validation,and deeper mechanistic exploration of the ubiquitin protein ligase E3C-signal transducer and activator of transcription 3 ubiquitination axis to establish the clinical and biological relevance of this pathway.
基金Supported by the National High Level Hospital Clinical Research Funding,No.2022-PUMCH-B-080 and No.2022-PUMCH-C-064.
文摘BACKGROUND Thin endometrium,leading cause of recurrent implantation failure and infertility,has been found to respond to exosomes.AIM To investigate the efficacy of exosomes in addressing the issue of thin endometrium.METHODS RNA sequencing and reverse transcription-quantitative polymerase chain reaction were employed to identify differentially expressed microRNAs(miRNAs)in human umbilical cord mesenchymal stem cell(hucMSC)treated with exosomes enriched with endometrial cell-derived components.Additionally,Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were conducted to highlight significant enrichment in specific biological pathways,molecular functions,and cellular components.Transwell and wound healing assays were performed to assess migratory potential,and western blotting was detected protein level.RESULTS A total of 53 differentially expressed miRNAs were identified in hucMSC treated with exosomes enriched with endometrial cell-derived components,comprising 27 upregulated and 26 downregulated miRNAs,which includes miR-137-3p.Enhanced migratory potential was observed in the Transwell and wound healing assays,and western blotting confirmed the epithelial differentiation of hucMSC and the increased p-signal transducer and activator of transcription 3.These effects were attributed to the upregulation of miR-137-3p.CONCLUSION miR-137-3p in exosomes from hypoxia-affected endometrial epithelial cell stimulates the signal transducer and activator of transcription 3 signaling pathway,enhancing the migration and differentiation of hucMSC into endometrial epithelial cell.
文摘This study by Zhang et al elucidates the role of exosome miR-137-3p targeting ubiquitin protein ligase E3C to activate signal transducer and activator of transcription 3 under hypoxia conditions,thereby promoting the migration and differentiation of human umbilical cord mesenchymal stem cells to endometrial epithelial cells.It emphasizes that exosomal miR-137-3p/ubiquitin protein ligase E3C/signal transducer and activator of transcription 3 axis is a promising pathway for endometrial regeneration.This article introduced the therapeutic potential of exosomal microRNAs in regenerative medicine while underscoring the need for standardized protocols in optimizing exosome delivery and validating molecular pathways for clinical translation.
文摘A family of high_order accuracy explicit difference schemes for solving 2_dimension parabolic P.D.E. are constructed. Th e stability condition is r=Δt/Δx 2=Δt/Δy 2【1/2 and the truncation err or is O(Δt 3+Δx 4).
文摘Two new freshwater atyid shrimps, Caridina angustipes sp nov.and C clavipes sp nov.from Hunan Province,China are described. C angustipes sp nov.is similar to C baojingensis Guo,He et Bai,1992,but with longer rostrum,15-27 dorsal teeth,3-6 ventral teeth,and straighter inner margin of endopods in male 1st pleopods. C clavipes sp nov.is similar to C angustipes ,but with narrower distal and fewer spines on inner margin of endopds in male 1st pleopods,and shorter and thinner appendix masculina in male 2nd pleopods with only 1 spine on inner lateral margin and 4 spines in apex.The holotypes are deposited in Shanghai Fisheries University.Some representative paratypes are deposited in Foshan Science and Technology College.
文摘The whole plant with root ofHedyotis diffusa Willd. （AE） has been demonstrated to have anti-inflammatory, antioxidant, and anti-bacterial activities. In our study, we aim to examine the anti-tumor effect of alcoholic extract of AE in mice implanted with sarcoma S180 cells （SBT mice）. We also compared the immune system function and the life span of SBT mice with that of control mice. We found that AE displayed a significant inhibitory effect on solid tumor growth, as well as increased the life span. At the dose of 10 mg/kg body weight, both tumor weight and volume were decreased significantly. We also measured several immune fimction markers, inlcuding spleen index （SI）, thymus index （TI） and parameters of hematological. We observed no reduce in these markers, indicatine that AE could inhibit tumor ~rowth without affectino immune function in SBT ,nice.
文摘Objective: To study the prognostic value of the pathological margin and molecular margin marked by eIF4E and P53 protein in laryngeal carcinoma. Methods: The prognostic value of pathological and molecular margin was studied in 253 cases and 67 cases respectively, the latter were pathological negative margin chosen from the former. Immunohistochemisty was used to detect the expression of eIF4E and p53 proteins. Results: The rate of pathological, p53 and eIF4E positive margins was 20.2%, 19.4% and 32.8% respectively. The recurrent rate of those with positive margins was higher than that of negative margins, which including pathological margin (70.6% vs 35.1%, P =0.0000), p53 margin (69.2% vs 33.3%, P =0.018) and eIF4E margin (63.6% vs 28.9%, P =0.018); The survival rate of those with negative margins was higher than those with positive margins, including pathological margin (the 5-year cumulative survival rate was 37.52% and 64.37% respectively, P =0.0023), p53 margin (the 5-year cumulative survival rate was 24.62% and 75.69% respectively, P =0.0012) and eIF4E margin (the 5-year cumulative survival rate was 43.31% and 77.52% respectively, P =0.0006). Conclusion: The prognosis of those with both pathological and molecular positive margins was worse than that of the negative margins; Both the eIF4E and p53 were useful markers to pick out the poor prognostic patients from those with pathological negative margin, and the former seemed to be more potential.
文摘The portal is characterized with large amount of information, high stability and interaction, while "Online Journal of Physical P.E" is to publicize the academic pure value, spread the achievement influence in daily life, and advocate an academic atmosphere of utilitarianism with a slogan of "article issued online can be published in journal with the recommendation of experts" and practical action. It is the first academic journal under network operation.
