Wheat root systems undergo dynamic and adaptive changes to mitigate adverse effects through sophisticated regulatory mechanisms under drought stress.Elucidating and utilizing these mechanisms is highly important for b...Wheat root systems undergo dynamic and adaptive changes to mitigate adverse effects through sophisticated regulatory mechanisms under drought stress.Elucidating and utilizing these mechanisms is highly important for breeding drought-resistant wheat varieties.Here,we identify histone deacetylase TaHDA8 as a critical component that negatively regulates wheat root elongation and drought tolerance.Under drought stress,TaHDA8 could be finely tuned to alleviate its inhibition of root elongation,thereby improving wheat adaptation to water deficit.Interestingly,reduction in TaHDA8 protein levels de-represses the DNA-binding ability of TaAREB3,a positive regulator of root elongation and drought tolerance,which depends on the retention of acetylation at K248 and K281 residues.The restored DNA binding of TaAREB3 to the promoter of TaKOR1 upregulates its expression,thereby promoting root elongation by enhancing cell proliferation in the root meristem.Further studies revealed that natural variations in the TaKOR1 promoter determine the differences in TaAREB3 binding,and wheat germplasm with TaHDA8–TaAREB3–TaKOR1 regulatory module has been widely selected.Collectively,this study reveals how a lysine deacetylase regulates drought-responsive root development via non-histone deacetylation,providing genetic resources for improving root architecture and breeding drought-resilient wheat varieties.展开更多
Wheat is a staple food globally.Grain size substantially affects yield by influencing grain weight.Only a few genes associated with grain yield have been cloned in wheat by omics strategies and map-based cloning.Lecti...Wheat is a staple food globally.Grain size substantially affects yield by influencing grain weight.Only a few genes associated with grain yield have been cloned in wheat by omics strategies and map-based cloning.Lectin receptor-like kinases(LecRLKs),a plant-specific RLK subfamily,are involved in plant development,seed germination,hormone signaling,and response to abiotic and biotic stresses[1].A recent study has indicated an association of LecRLKs with grain yield[2].However,the role of wheat LecRLKs in the regulation of grain size remains poorly understood.展开更多
基金funded by the National Natural Science Foundation of China(32130078,32441061)the National Key Research and Development Program of China(2022YFF1001604)+1 种基金Chinese Universities Scientific Fund(2024TC188)Young-scholar program of China Agricultural University-Bayannur Research Institute(2024BYNECAU009).
文摘Wheat root systems undergo dynamic and adaptive changes to mitigate adverse effects through sophisticated regulatory mechanisms under drought stress.Elucidating and utilizing these mechanisms is highly important for breeding drought-resistant wheat varieties.Here,we identify histone deacetylase TaHDA8 as a critical component that negatively regulates wheat root elongation and drought tolerance.Under drought stress,TaHDA8 could be finely tuned to alleviate its inhibition of root elongation,thereby improving wheat adaptation to water deficit.Interestingly,reduction in TaHDA8 protein levels de-represses the DNA-binding ability of TaAREB3,a positive regulator of root elongation and drought tolerance,which depends on the retention of acetylation at K248 and K281 residues.The restored DNA binding of TaAREB3 to the promoter of TaKOR1 upregulates its expression,thereby promoting root elongation by enhancing cell proliferation in the root meristem.Further studies revealed that natural variations in the TaKOR1 promoter determine the differences in TaAREB3 binding,and wheat germplasm with TaHDA8–TaAREB3–TaKOR1 regulatory module has been widely selected.Collectively,this study reveals how a lysine deacetylase regulates drought-responsive root development via non-histone deacetylation,providing genetic resources for improving root architecture and breeding drought-resilient wheat varieties.
基金funded by the National Key Research and Development Program(2023YFD1200403)the Henan Natural Science Foundation(24HASTIT055,252300421246,and 25A210020)of China.
文摘Wheat is a staple food globally.Grain size substantially affects yield by influencing grain weight.Only a few genes associated with grain yield have been cloned in wheat by omics strategies and map-based cloning.Lectin receptor-like kinases(LecRLKs),a plant-specific RLK subfamily,are involved in plant development,seed germination,hormone signaling,and response to abiotic and biotic stresses[1].A recent study has indicated an association of LecRLKs with grain yield[2].However,the role of wheat LecRLKs in the regulation of grain size remains poorly understood.
