Understanding the intra-population genetic diversity and population genetic structure is beneficial for plant protection and breeding.Dendrobium huoshanense is an endangered authentic medicinal herb.To analyze the pop...Understanding the intra-population genetic diversity and population genetic structure is beneficial for plant protection and breeding.Dendrobium huoshanense is an endangered authentic medicinal herb.To analyze the population differentiation and genetic diversity in D.huoshanense,simple sequence repeat(SSR)markers were obtained by high-throughput transcriptome sequencing,and 10 expressed sequence tag(EST)-SSRs were studied in 182 Dendrobium individual samples from 15 populations distributed in Huoshan County,southwest Anhui Province.After deep sequencing of D.huoshanense transcriptome,a total of 58202 unigenes were obtained,and 7781 SSRs with 1~6 base repeats were detected.Among them,dinucleotide SSRs were the dominant repeating motifs(3119,40.085%),and the most common was AG/CT(2666,54.01%).Ten SSR primers were screened out from the designed 120 primer pairs for further polymorphism verification.The results showed that 75 alleles were detected,and the number of alleles at each locus ranged from 4 to 11,with an average of 7.5.The mean expected heterozygosity(H_(e)),observed heterozygosity(H_(o))and polymorphism information content(C_(pi))of the 10 SSR loci were 0.646,0.574,and 0.607,respectively.D.huoshanense populations exhibited a high level of genetic diversity(H_(o)=0.591,H_(e)=0.557)and a moderate level of differentiation(F_(ST)=0.10069),and the genetic variation mainly existed within populations.The results of Structure and neighbour-joining(NJ)analysis based on 10 polymorphic primers showed that D.huoshanense populations were clustered into 2 groups,and the populations KS,HSD,and JXZ had rich genetic diversity and multiple private alleles,suggesting that these populations could be used for genetic improvement of the species.The study revealed frequency distribution of D.huoshanense SSR loci,and successfully developed microsatellite genetic markers,which are expected to expand the repertoire of DNA markers for genetic study of D.huoshanense.展开更多
Genetic mapping provides a powerful tool for the analysis of quantitative trait loci （QTLs） at the genomic level. Herein, we report a new genetic linkage map developed from an F1-derived doubled haploid （DH） popul...Genetic mapping provides a powerful tool for the analysis of quantitative trait loci （QTLs） at the genomic level. Herein, we report a new genetic linkage map developed from an F1-derived doubled haploid （DH） population of 168 lines, which was generated from the cross between two elite Chinese common wheat （Triticum aestivum L.） varieties, Huapei 3 and Yumai 57. The map contained 305 loci, represented by 283 simple sequence repeat （SSR） and 22 expressed sequence tag （EST）-SSR markers, which covered a total length of 2141.7 cM with an average distance of 7.02 cM between adjacent markers on the map. The chromosomal locations and map positions of 22 new SSR markers were determined, and were found to distribute on 14 linkage groups. Twenty SSR loci showed different chromosomal locations from those reported in other maps. Therefore, this map offers new information on the SSR markers of wheat. This genetic map provides new opportunities to detect and map QTLs controlling agronomically important traits. The unique features of this map are discussed.展开更多
文摘Understanding the intra-population genetic diversity and population genetic structure is beneficial for plant protection and breeding.Dendrobium huoshanense is an endangered authentic medicinal herb.To analyze the population differentiation and genetic diversity in D.huoshanense,simple sequence repeat(SSR)markers were obtained by high-throughput transcriptome sequencing,and 10 expressed sequence tag(EST)-SSRs were studied in 182 Dendrobium individual samples from 15 populations distributed in Huoshan County,southwest Anhui Province.After deep sequencing of D.huoshanense transcriptome,a total of 58202 unigenes were obtained,and 7781 SSRs with 1~6 base repeats were detected.Among them,dinucleotide SSRs were the dominant repeating motifs(3119,40.085%),and the most common was AG/CT(2666,54.01%).Ten SSR primers were screened out from the designed 120 primer pairs for further polymorphism verification.The results showed that 75 alleles were detected,and the number of alleles at each locus ranged from 4 to 11,with an average of 7.5.The mean expected heterozygosity(H_(e)),observed heterozygosity(H_(o))and polymorphism information content(C_(pi))of the 10 SSR loci were 0.646,0.574,and 0.607,respectively.D.huoshanense populations exhibited a high level of genetic diversity(H_(o)=0.591,H_(e)=0.557)and a moderate level of differentiation(F_(ST)=0.10069),and the genetic variation mainly existed within populations.The results of Structure and neighbour-joining(NJ)analysis based on 10 polymorphic primers showed that D.huoshanense populations were clustered into 2 groups,and the populations KS,HSD,and JXZ had rich genetic diversity and multiple private alleles,suggesting that these populations could be used for genetic improvement of the species.The study revealed frequency distribution of D.huoshanense SSR loci,and successfully developed microsatellite genetic markers,which are expected to expand the repertoire of DNA markers for genetic study of D.huoshanense.
基金the National Natural Science Foundation of China (30671270)the Hi-Tech Research and Development (863) Program of China(2006AA10Z1E9 and 2006AA100101)
文摘Genetic mapping provides a powerful tool for the analysis of quantitative trait loci （QTLs） at the genomic level. Herein, we report a new genetic linkage map developed from an F1-derived doubled haploid （DH） population of 168 lines, which was generated from the cross between two elite Chinese common wheat （Triticum aestivum L.） varieties, Huapei 3 and Yumai 57. The map contained 305 loci, represented by 283 simple sequence repeat （SSR） and 22 expressed sequence tag （EST）-SSR markers, which covered a total length of 2141.7 cM with an average distance of 7.02 cM between adjacent markers on the map. The chromosomal locations and map positions of 22 new SSR markers were determined, and were found to distribute on 14 linkage groups. Twenty SSR loci showed different chromosomal locations from those reported in other maps. Therefore, this map offers new information on the SSR markers of wheat. This genetic map provides new opportunities to detect and map QTLs controlling agronomically important traits. The unique features of this map are discussed.
