After gene mutation, the pcDNA3.1/APP595/596 plasmid was transfected into HEK293 cells to establish a cell model of Alzheimer＇s disease. The cell model was treated with donepezil or compound Danshen tablets after cul...After gene mutation, the pcDNA3.1/APP595/596 plasmid was transfected into HEK293 cells to establish a cell model of Alzheimer＇s disease. The cell model was treated with donepezil or compound Danshen tablets after culture for 72 hours. Reverse transcription-PCR showed that the mRNA expression of amyloid protein precursor decreased in all groups following culture for 24 hours, and that there was no significant difference in the amount of decrease between donepezil and compound Danshen tablets. Our results suggest that compound Danshen tablets can reduce expression of the mRNA for amyloid protein precursor in a transgenic cell model of Alzheimer＇s disease, with similar effects to donepezil.展开更多
The phenomenon of car-following is special in traffic operations. Traditional car-following models can well describe the reactions of the movements between two concessive vehicles in the same lane within a certain dis...The phenomenon of car-following is special in traffic operations. Traditional car-following models can well describe the reactions of the movements between two concessive vehicles in the same lane within a certain distance. With the invention of connected vehicle technologies, more and more advisory messages are in development and applied in our daily lives, some of which are related to the measures and warnings of speed and headway distance between the two concessive vehicles. Such warnings may change the conventional car-following mechanisms. This paper intends to consider the possible impacts of in-vehicle warning messages to improve the traditional car-following models, including the General Motor (GM) Model and the Linear (Helly) Model, by calibrating model parameters using field data from an arterial road in Houston, Texas, U.S.A. The safety messages were provided by a tablet/smartphone application. One exponent was applied to the GM model, while another one applied to the Linear (Helly) model, both were on the stimuli term “difference in velocity between two concessive vehicles”. The calibration and validation were separately conducted for deceleration and acceleration conditions. Results showed that, the parameters of the traditional GM model failed to be properly calibrated with the interference of in-vehicle safety messages, and the parameters calibrated from the traditional Linear (Helly) Model with no in-vehicle messages could not be directly used in the case with such messages. However, both updated models can be well calibrated even if those messages were provided. The entire research process, as well as the calibrated models and parameters could be a reference in the on-going connected vehicle program and micro/macroscopic traffic simulations.展开更多
目的:评估乌梢祛风片联合甲氨蝶呤片治疗类风湿关节炎(RA)的临床疗效与经济性。方法:通过医院信息系统,随机抽取符合标准的RA患者,根据用药情况分为乌梢祛风片组(简称“蛇片组”)和对照组。所有患者均采用甲氨蝶呤片口服治疗,蛇片组患...目的:评估乌梢祛风片联合甲氨蝶呤片治疗类风湿关节炎(RA)的临床疗效与经济性。方法:通过医院信息系统,随机抽取符合标准的RA患者,根据用药情况分为乌梢祛风片组(简称“蛇片组”)和对照组。所有患者均采用甲氨蝶呤片口服治疗,蛇片组患者在此基础上给予乌梢祛风片口服治疗。利用倾向性匹配使两组患者的基线数据一致,比较两组的中医证候疗效、疾病缓解情况等疗效指标。使用Treeage pro 2022软件构建决策树模型,进行成本-效果分析及敏感性分析。结果:通过倾向性匹配,最终纳入蛇片组与对照组患者各53例。蛇片组患者的中医证候疗效总有效率为84.91%,对照组为67.92%,蛇片组的疗效优于对照组(P<0.05)。蛇片组在减少触痛关节数、肿胀关节数方面优于对照组(P<0.05)。成本-效果分析表明,蛇片组的增量成本为1299.01元,基于中医证候疗效与ACR20疗效的增量成本效果比分别为92.79与68.37。敏感性分析表明,蛇片组住院天数和日均住院费用影响大;当患者的意愿支付阈值为56295.54元时,经济性更优。结论:乌梢祛风片联合甲氨蝶呤片治疗方案能有效改善RA患者的中医证候、缓解关节症状,且联合治疗比单独使用甲氨蝶呤片更具经济性。展开更多
目的运用网状Meta分析评估不同中成药联合化学药治疗甲亢的疗效与安全性,为临床用药提供循证依据。方法计算机检索VIP、Wanfang、CNKI、PubMed、Cochrane Library、Web of Science等数据库,检索公开发表中成药治疗甲亢的对照研究,检索...目的运用网状Meta分析评估不同中成药联合化学药治疗甲亢的疗效与安全性,为临床用药提供循证依据。方法计算机检索VIP、Wanfang、CNKI、PubMed、Cochrane Library、Web of Science等数据库,检索公开发表中成药治疗甲亢的对照研究,检索时限建库至2025年1月。采用Cochrane 5.4手册对纳入的研究进行质量评价。运用R4.1.1软件进行贝叶斯网状Meta数据结果比较及排序。结果最终纳入31篇研究,总样本量2615例,共纳入9种口服中成药。网状Meta分析结果表明,有效率排名前三的干预措施为:百令胶囊+化学药>知柏地黄丸(口服液)+化学药>雷公藤多苷片(多甙片)+化学药;降低FT3激素水平前三排序:雷公藤多苷片(多甙片)+化学药>玄栀颗粒+化学药>甲亢平消丸+化学药;降低FT4激素水平前三排序:雷公藤多苷片(多甙片)+化学药>百令胶囊+化学药>玄栀颗粒+化学药;提高TSH激素水平前三排序:玄栀颗粒+化学药>百令胶囊+化学药>甲亢平消丸+化学药;降低TT3激素水平前三排序:甲亢灵胶囊+化学药>百令胶囊+化学药>知柏地黄丸(口服液)+化学药;降低TT4激素水平前三排序:百令胶囊+化学药>知柏地黄丸(口服液)+化学药>银甲散(丹)+化学药。结论治疗甲状腺功能亢进采用口服中成药的方案中,提高有效率及降低TT4激素水平方面,首选百令胶囊+化学药;针对改善相关激素水平方面,在降低FT3、FT4激素水平方面,雷公藤多苷片(多甙片)+化学药最有效,甲亢灵胶囊+化学药在降低TT3激素水平方面最有效,临床中可根据患者的具体情况合理准确选择用药。展开更多
For several years, near-infrared spectroscopy (NIRS) has become an analytical technique of great interest for the pharmaceutical industry, particularly for the non-destructive analysis of dosage forms. The goal of thi...For several years, near-infrared spectroscopy (NIRS) has become an analytical technique of great interest for the pharmaceutical industry, particularly for the non-destructive analysis of dosage forms. The goal of this study is to show the capacity of this new technique to assay the active ingredient in low-dosage tablets. NIR spectroscopy is a rapid, non-destructive technique and does not need any sample preparation. A prediction model was built by using a partial least square regression fit method. The NIR assay was performed by transmission. The results obtained by NIR spectroscopy were compared with the conventional HPLC method for Montelukast tablets produced by Sigma pharmaceutical corp. The study showed that Montelukast tablets can be individually analyzed by NIR with high accuracy. It was shown that the variability of this new tech- nique is less important than that of the conventional method which is the HPLC with UV detection.展开更多
Objective Hypoglycemic Ziyabiti Tablets(HZT)is a traditional multicomponent treatment for diabetes in Xinjiang Uyghur Traditional Medicine.The present study aimed to investigate the effect of HZT in diabetic rats and ...Objective Hypoglycemic Ziyabiti Tablets(HZT)is a traditional multicomponent treatment for diabetes in Xinjiang Uyghur Traditional Medicine.The present study aimed to investigate the effect of HZT in diabetic rats and its activity in cultured liver cells to investigate the relative mechanisms.Methods 10 days high-fat diet fed rats were intraperitoneally injected with alloxan(ALX)at next two subsequent days to induce diabetes mellitus(DM).Then were divided into 5 groups:saline,positive DM control and DM groups treated with different doses of HZT.Fasting blood glucose(FBG),total cholesterol(TC),total triglycerides(TG),high-density lipoprotein(HDL-C),fasting insulin(FI),insulin secretion(IS)and insulin sensitivity index(ISI)were measured.The IC_(50) of HZT in L-02 cells was determined by MTT assay,in intact and in paracetamol-induced liver injury(Par),on lactate dehydrogenase(LDH)activity and on glucose consumption.Results HZT decreased FBG and TC(P <0.05),increased IS(P <0.05)and at 440 mg·kg^(-1)·d^(-1) increased FI(P < 0.01).In vitro,HZT at 0.1,0.2,and 0.4 mg/mL decreased LDH activity and promoted glucose consumption.Conclusion The hypoglycemic mechanism of HZT is possibly related to increased insulin secretion from the pancreas and increased utilization of glucose by the liver.展开更多
The objective of this study was to develop once-daily metformin hydrochloride sustained-release tablets (MHSRT) and evaluate their in vitro release behavior. MHSRT were prepared by the film coating method. The in vitr...The objective of this study was to develop once-daily metformin hydrochloride sustained-release tablets (MHSRT) and evaluate their in vitro release behavior. MHSRT were prepared by the film coating method. The in vitro drug release rate of MHSRT and the commercial tablets Fortamet? made in the United States of America in water was fitted with zero order kinetic equation, and Ritger-Peppas kinetic equation in 0.1 M HCl and pH 6.8-phosphate buffer, respectively. The similarity factor f2 values of MHSRT in three different dissolution medium were 82, 80 and 74, respectively in comparison with imported Fortamet?, which were all greater than 50. The results of storage-stability showed that MHSRT were stable for at least 6 months under stress condition (40℃ ± 2℃, RH 75% ± 5%). Therefore, in this study, MHSRT were successfully prepared using optimized formulation technologies that meet mass produce. The in vitro release behavior of MHSRT was almost similar to that of imported Fortamet?.展开更多
目的建立窄治疗指数药物华法林钠片临床相关的质量标准。方法利用GastroplusTM软件构建华法林钠片在健康成人中的口服生理药动学(physiologically based pharmacokinetic,PBPK)模型,选择合适的溶出模型建立药物体外和体内关系(in vitro ...目的建立窄治疗指数药物华法林钠片临床相关的质量标准。方法利用GastroplusTM软件构建华法林钠片在健康成人中的口服生理药动学(physiologically based pharmacokinetic,PBPK)模型,选择合适的溶出模型建立药物体外和体内关系(in vitro and in vivo relationships,IVIVR),结合虚拟生物等效性研究(virtual bioequivalence,VBE)方法,探讨华法林钠片(2.5 mg)临床相关的溶出度和含量安全空间。结果成功建立并验证了华法林钠片的口服PBPK模型。使用Z-factor和weibull模型建立了不同溶出条件下的华法林钠片的IVIVR。VBE模拟结果表明,在满足生物等效性前提下,华法林钠片(2.5 mg)的溶出度安全空间为pH 6.8溶出介质条件下,Q_(30 min)≥80%,含量安全空间范围为95%~105%,超出以上范围可能导致生物不等效的风险。结论本研究建立了一种基于PBPK模型的药物质量标准评价方法,为临床相关药品质量标准的制定提供了新的思路和科学依据,帮助提高药品质量研究和药品监管效率。展开更多
基金supported by the Bureau of Traditional Chinese Medicine of Guangdong Province, No. 2010463the National Science and Technology"12~(th) Five-years"Major Special-purpose Foundation,No.2011ZX09201-201-01
文摘After gene mutation, the pcDNA3.1/APP595/596 plasmid was transfected into HEK293 cells to establish a cell model of Alzheimer＇s disease. The cell model was treated with donepezil or compound Danshen tablets after culture for 72 hours. Reverse transcription-PCR showed that the mRNA expression of amyloid protein precursor decreased in all groups following culture for 24 hours, and that there was no significant difference in the amount of decrease between donepezil and compound Danshen tablets. Our results suggest that compound Danshen tablets can reduce expression of the mRNA for amyloid protein precursor in a transgenic cell model of Alzheimer＇s disease, with similar effects to donepezil.
文摘The phenomenon of car-following is special in traffic operations. Traditional car-following models can well describe the reactions of the movements between two concessive vehicles in the same lane within a certain distance. With the invention of connected vehicle technologies, more and more advisory messages are in development and applied in our daily lives, some of which are related to the measures and warnings of speed and headway distance between the two concessive vehicles. Such warnings may change the conventional car-following mechanisms. This paper intends to consider the possible impacts of in-vehicle warning messages to improve the traditional car-following models, including the General Motor (GM) Model and the Linear (Helly) Model, by calibrating model parameters using field data from an arterial road in Houston, Texas, U.S.A. The safety messages were provided by a tablet/smartphone application. One exponent was applied to the GM model, while another one applied to the Linear (Helly) model, both were on the stimuli term “difference in velocity between two concessive vehicles”. The calibration and validation were separately conducted for deceleration and acceleration conditions. Results showed that, the parameters of the traditional GM model failed to be properly calibrated with the interference of in-vehicle safety messages, and the parameters calibrated from the traditional Linear (Helly) Model with no in-vehicle messages could not be directly used in the case with such messages. However, both updated models can be well calibrated even if those messages were provided. The entire research process, as well as the calibrated models and parameters could be a reference in the on-going connected vehicle program and micro/macroscopic traffic simulations.
文摘目的:评估乌梢祛风片联合甲氨蝶呤片治疗类风湿关节炎(RA)的临床疗效与经济性。方法:通过医院信息系统,随机抽取符合标准的RA患者,根据用药情况分为乌梢祛风片组(简称“蛇片组”)和对照组。所有患者均采用甲氨蝶呤片口服治疗,蛇片组患者在此基础上给予乌梢祛风片口服治疗。利用倾向性匹配使两组患者的基线数据一致,比较两组的中医证候疗效、疾病缓解情况等疗效指标。使用Treeage pro 2022软件构建决策树模型,进行成本-效果分析及敏感性分析。结果:通过倾向性匹配,最终纳入蛇片组与对照组患者各53例。蛇片组患者的中医证候疗效总有效率为84.91%,对照组为67.92%,蛇片组的疗效优于对照组(P<0.05)。蛇片组在减少触痛关节数、肿胀关节数方面优于对照组(P<0.05)。成本-效果分析表明,蛇片组的增量成本为1299.01元,基于中医证候疗效与ACR20疗效的增量成本效果比分别为92.79与68.37。敏感性分析表明,蛇片组住院天数和日均住院费用影响大;当患者的意愿支付阈值为56295.54元时,经济性更优。结论:乌梢祛风片联合甲氨蝶呤片治疗方案能有效改善RA患者的中医证候、缓解关节症状,且联合治疗比单独使用甲氨蝶呤片更具经济性。
文摘目的运用网状Meta分析评估不同中成药联合化学药治疗甲亢的疗效与安全性,为临床用药提供循证依据。方法计算机检索VIP、Wanfang、CNKI、PubMed、Cochrane Library、Web of Science等数据库,检索公开发表中成药治疗甲亢的对照研究,检索时限建库至2025年1月。采用Cochrane 5.4手册对纳入的研究进行质量评价。运用R4.1.1软件进行贝叶斯网状Meta数据结果比较及排序。结果最终纳入31篇研究,总样本量2615例,共纳入9种口服中成药。网状Meta分析结果表明,有效率排名前三的干预措施为:百令胶囊+化学药>知柏地黄丸(口服液)+化学药>雷公藤多苷片(多甙片)+化学药;降低FT3激素水平前三排序:雷公藤多苷片(多甙片)+化学药>玄栀颗粒+化学药>甲亢平消丸+化学药;降低FT4激素水平前三排序:雷公藤多苷片(多甙片)+化学药>百令胶囊+化学药>玄栀颗粒+化学药;提高TSH激素水平前三排序:玄栀颗粒+化学药>百令胶囊+化学药>甲亢平消丸+化学药;降低TT3激素水平前三排序:甲亢灵胶囊+化学药>百令胶囊+化学药>知柏地黄丸(口服液)+化学药;降低TT4激素水平前三排序:百令胶囊+化学药>知柏地黄丸(口服液)+化学药>银甲散(丹)+化学药。结论治疗甲状腺功能亢进采用口服中成药的方案中,提高有效率及降低TT4激素水平方面,首选百令胶囊+化学药;针对改善相关激素水平方面,在降低FT3、FT4激素水平方面,雷公藤多苷片(多甙片)+化学药最有效,甲亢灵胶囊+化学药在降低TT3激素水平方面最有效,临床中可根据患者的具体情况合理准确选择用药。
文摘For several years, near-infrared spectroscopy (NIRS) has become an analytical technique of great interest for the pharmaceutical industry, particularly for the non-destructive analysis of dosage forms. The goal of this study is to show the capacity of this new technique to assay the active ingredient in low-dosage tablets. NIR spectroscopy is a rapid, non-destructive technique and does not need any sample preparation. A prediction model was built by using a partial least square regression fit method. The NIR assay was performed by transmission. The results obtained by NIR spectroscopy were compared with the conventional HPLC method for Montelukast tablets produced by Sigma pharmaceutical corp. The study showed that Montelukast tablets can be individually analyzed by NIR with high accuracy. It was shown that the variability of this new tech- nique is less important than that of the conventional method which is the HPLC with UV detection.
文摘Objective Hypoglycemic Ziyabiti Tablets(HZT)is a traditional multicomponent treatment for diabetes in Xinjiang Uyghur Traditional Medicine.The present study aimed to investigate the effect of HZT in diabetic rats and its activity in cultured liver cells to investigate the relative mechanisms.Methods 10 days high-fat diet fed rats were intraperitoneally injected with alloxan(ALX)at next two subsequent days to induce diabetes mellitus(DM).Then were divided into 5 groups:saline,positive DM control and DM groups treated with different doses of HZT.Fasting blood glucose(FBG),total cholesterol(TC),total triglycerides(TG),high-density lipoprotein(HDL-C),fasting insulin(FI),insulin secretion(IS)and insulin sensitivity index(ISI)were measured.The IC_(50) of HZT in L-02 cells was determined by MTT assay,in intact and in paracetamol-induced liver injury(Par),on lactate dehydrogenase(LDH)activity and on glucose consumption.Results HZT decreased FBG and TC(P <0.05),increased IS(P <0.05)and at 440 mg·kg^(-1)·d^(-1) increased FI(P < 0.01).In vitro,HZT at 0.1,0.2,and 0.4 mg/mL decreased LDH activity and promoted glucose consumption.Conclusion The hypoglycemic mechanism of HZT is possibly related to increased insulin secretion from the pancreas and increased utilization of glucose by the liver.
文摘The objective of this study was to develop once-daily metformin hydrochloride sustained-release tablets (MHSRT) and evaluate their in vitro release behavior. MHSRT were prepared by the film coating method. The in vitro drug release rate of MHSRT and the commercial tablets Fortamet? made in the United States of America in water was fitted with zero order kinetic equation, and Ritger-Peppas kinetic equation in 0.1 M HCl and pH 6.8-phosphate buffer, respectively. The similarity factor f2 values of MHSRT in three different dissolution medium were 82, 80 and 74, respectively in comparison with imported Fortamet?, which were all greater than 50. The results of storage-stability showed that MHSRT were stable for at least 6 months under stress condition (40℃ ± 2℃, RH 75% ± 5%). Therefore, in this study, MHSRT were successfully prepared using optimized formulation technologies that meet mass produce. The in vitro release behavior of MHSRT was almost similar to that of imported Fortamet?.
文摘目的建立窄治疗指数药物华法林钠片临床相关的质量标准。方法利用GastroplusTM软件构建华法林钠片在健康成人中的口服生理药动学(physiologically based pharmacokinetic,PBPK)模型,选择合适的溶出模型建立药物体外和体内关系(in vitro and in vivo relationships,IVIVR),结合虚拟生物等效性研究(virtual bioequivalence,VBE)方法,探讨华法林钠片(2.5 mg)临床相关的溶出度和含量安全空间。结果成功建立并验证了华法林钠片的口服PBPK模型。使用Z-factor和weibull模型建立了不同溶出条件下的华法林钠片的IVIVR。VBE模拟结果表明,在满足生物等效性前提下,华法林钠片(2.5 mg)的溶出度安全空间为pH 6.8溶出介质条件下,Q_(30 min)≥80%,含量安全空间范围为95%~105%,超出以上范围可能导致生物不等效的风险。结论本研究建立了一种基于PBPK模型的药物质量标准评价方法,为临床相关药品质量标准的制定提供了新的思路和科学依据,帮助提高药品质量研究和药品监管效率。
