The emergence of wearable devices capable of continuous biomarker monitoring has significantly transformed the traditional landscape of healthcare,offering real-time insights into physiological changes,enabling early ...The emergence of wearable devices capable of continuous biomarker monitoring has significantly transformed the traditional landscape of healthcare,offering real-time insights into physiological changes,enabling early detection of disease,and facilitating individualized therapeutic.Nucleic acid aptamers,as a highly sensitive biorecognition elements with programmable properties,provide an innovative solution for building a new generation of continuous monitoring system.This review presents recent developments in aptamers-based wearable electrochemical sensors for continuous monitoring of biomarkers.Firstly,the core advantages of aptamers in continuous monitoring are summarized,including unique reversible binding properties,excellent biostability and engineerable modification ability,which make them ideal recognition elements for building real-time biosensing interfaces.Secondly,this review provides insights into the design principles of aptamers-based electrochemical sensors,including the characterization of aptamers,the immobilization methods on the electrode surface and the sensing strategies of sensors.Besides,this review highlights recent research advances in the field,focusing on the design of different types of sensors as well as their applications.Specifically,according to the difference target acquisition methods,the type of sensors is classified into non-invasive sensors,which involve monitoring biomarkers present in accessible biofluids such as sweat,saliva and wound exudate,and low-invasive sensors utilizing microneedle patch technology to sample biomarkers within interstitial fluids.Finally,this review introduces the challenges and discuss potential solutions for future development of aptamers-based wearable biosensors,including how to achieve effective biomarkers detection,ensure reversible binding response and ensure long-term stability,etc.This review provides useful reference for the development of aptamers-based electrochemical continuous monitoring technology in healthcare applications.展开更多
Bionic limbs require reliable,low-noise and high-comfort interfaces between electrodes and the prosthetic system.This work presents the first fully flexible,wearable myoelectric control system compatible with both dry...Bionic limbs require reliable,low-noise and high-comfort interfaces between electrodes and the prosthetic system.This work presents the first fully flexible,wearable myoelectric control system compatible with both dry and wet electrodes.It features a low-noise front-end circuit on foil using amorphous Indium-Gallium-Zinc-Oxide(a-IGZO)Thin-Film Transistors,optimized for multi-electrode sensing.The design includes an autozeroed pre-charging buffer to minimize offset and 1/f noise while maintaining high input impedance(841 MΩat 50 Hz).The front-end achieves 22µVrms input noise,<-90 dBc crosstalk,and a 4.6 mV input offset consuming 55.3µW per channel.EMG signals measured by this AFE were used to drive an elbow musculoskeletal model and predict the resulting human elbow flexion-extension moments,which in turn were used to realize a closed-loop real-time control in a simulated bionic elbow joint,using both dry and wet electrodes.Experiments done with a series of movements show a 20°rms error in angular control.展开更多
Recent researchhas shown that probiotics,particularly Lactobacillus sp.,have potential as modulators of the immune system.This review explores the immunotherapeutic potential of Lactobacillus species via adjuvant ther...Recent researchhas shown that probiotics,particularly Lactobacillus sp.,have potential as modulators of the immune system.This review explores the immunotherapeutic potential of Lactobacillus species via adjuvant therapy with immune checkpoint inhibitors(ICIs),such as programmed cell death protein-1,programmed death ligand-1,and cytotoxic T-lymphocyte antigen-4 inhibitors,which have revolutionized cancer treatment by enhancing T-cell-mediated antitumor immunity.However,patient response remains questionable,prompting further study into the role of the gut microbiota in modulating these therapies.These probiotics influence immune responses through interactions with immune cells such as dendritic cells,macrophages,and T cells.These interactions promote cytokine production,enhance CD8+T-cell activity,and improve the gut barrier.Specific Lactobacillus strains can increase ICI efficacy through the production of proinflammatory cytokines and associated side effects.Optimizing Lactobacillus-based therapies and integrating them with existing cancer treatments may lead to improved patient outcomes.Although the formulation of probiotic species in advanced drug delivery systems,such as niosomes and liposomes,may prove to be successful,regulatory guidelines need to be followed to support their nontoxic effects in preclinical trials followed by clinical studies.Although the concomitant administration of probiotics with ICIs remains a promising strategy,the underlying mechanisms need to be explored to support their systemic nontoxic effects.展开更多
基金support from the National Natural Science Foundation of China(Grant No.T2225010,32171399,32171456,32401202)Guangdong Basic and Applied Basic Research Foundation(Grant No.2023A1515011267,2023A1515111139,2025A1515010608)+4 种基金Science and Technology Program of Guangzhou,China(Grant No.2024B03J0121,2024B03J1284)Shenzhen Science and Technology Program(Grant No.RCBS20231211090558093)China Postdoctoral Science Foundation(2023TQ0386)Fundamental Research Funds for the Central Universities,Sun Yat-sen University(No.24xkjc011)the Opening Project of The National Key Laboratory of Smart Farm Technology and Systems.
文摘The emergence of wearable devices capable of continuous biomarker monitoring has significantly transformed the traditional landscape of healthcare,offering real-time insights into physiological changes,enabling early detection of disease,and facilitating individualized therapeutic.Nucleic acid aptamers,as a highly sensitive biorecognition elements with programmable properties,provide an innovative solution for building a new generation of continuous monitoring system.This review presents recent developments in aptamers-based wearable electrochemical sensors for continuous monitoring of biomarkers.Firstly,the core advantages of aptamers in continuous monitoring are summarized,including unique reversible binding properties,excellent biostability and engineerable modification ability,which make them ideal recognition elements for building real-time biosensing interfaces.Secondly,this review provides insights into the design principles of aptamers-based electrochemical sensors,including the characterization of aptamers,the immobilization methods on the electrode surface and the sensing strategies of sensors.Besides,this review highlights recent research advances in the field,focusing on the design of different types of sensors as well as their applications.Specifically,according to the difference target acquisition methods,the type of sensors is classified into non-invasive sensors,which involve monitoring biomarkers present in accessible biofluids such as sweat,saliva and wound exudate,and low-invasive sensors utilizing microneedle patch technology to sample biomarkers within interstitial fluids.Finally,this review introduces the challenges and discuss potential solutions for future development of aptamers-based wearable biosensors,including how to achieve effective biomarkers detection,ensure reversible binding response and ensure long-term stability,etc.This review provides useful reference for the development of aptamers-based electrochemical continuous monitoring technology in healthcare applications.
基金project Smart-Sense(with project number17608)which is(partly)financed by the Dutch Research Council(NWO)。
文摘Bionic limbs require reliable,low-noise and high-comfort interfaces between electrodes and the prosthetic system.This work presents the first fully flexible,wearable myoelectric control system compatible with both dry and wet electrodes.It features a low-noise front-end circuit on foil using amorphous Indium-Gallium-Zinc-Oxide(a-IGZO)Thin-Film Transistors,optimized for multi-electrode sensing.The design includes an autozeroed pre-charging buffer to minimize offset and 1/f noise while maintaining high input impedance(841 MΩat 50 Hz).The front-end achieves 22µVrms input noise,<-90 dBc crosstalk,and a 4.6 mV input offset consuming 55.3µW per channel.EMG signals measured by this AFE were used to drive an elbow musculoskeletal model and predict the resulting human elbow flexion-extension moments,which in turn were used to realize a closed-loop real-time control in a simulated bionic elbow joint,using both dry and wet electrodes.Experiments done with a series of movements show a 20°rms error in angular control.
文摘Recent researchhas shown that probiotics,particularly Lactobacillus sp.,have potential as modulators of the immune system.This review explores the immunotherapeutic potential of Lactobacillus species via adjuvant therapy with immune checkpoint inhibitors(ICIs),such as programmed cell death protein-1,programmed death ligand-1,and cytotoxic T-lymphocyte antigen-4 inhibitors,which have revolutionized cancer treatment by enhancing T-cell-mediated antitumor immunity.However,patient response remains questionable,prompting further study into the role of the gut microbiota in modulating these therapies.These probiotics influence immune responses through interactions with immune cells such as dendritic cells,macrophages,and T cells.These interactions promote cytokine production,enhance CD8+T-cell activity,and improve the gut barrier.Specific Lactobacillus strains can increase ICI efficacy through the production of proinflammatory cytokines and associated side effects.Optimizing Lactobacillus-based therapies and integrating them with existing cancer treatments may lead to improved patient outcomes.Although the formulation of probiotic species in advanced drug delivery systems,such as niosomes and liposomes,may prove to be successful,regulatory guidelines need to be followed to support their nontoxic effects in preclinical trials followed by clinical studies.Although the concomitant administration of probiotics with ICIs remains a promising strategy,the underlying mechanisms need to be explored to support their systemic nontoxic effects.
