Multi-organ-on-a-chip(MOOC)technology represents a pivotal direction in the organ-on-a-chip field,seeking to emulate the complex interactions of multiple human organs in vitro through microfluidic systems.This technol...Multi-organ-on-a-chip(MOOC)technology represents a pivotal direction in the organ-on-a-chip field,seeking to emulate the complex interactions of multiple human organs in vitro through microfluidic systems.This technology overcomes the limitations of traditional single-organ models,providing a novel platform for investigating complex disease mechanisms and evaluating drug efficacy and toxicity.Although it demonstrates broad application prospects,its development still faces critical bottlenecks,including inadequate physiological coupling between organs,short functional maintenance durations,and limited real-time monitoring capabilities.Contemporary research is advancing along three key directions,including functional coupling,sensor integration,and full-process automation systems,to propel the technology toward enhanced levels of physiological relevance and predictive accuracy.展开更多
Introduction:Traditional dengue surveillance operates reactively,frequently lagging behind viral transmission patterns and thereby impeding timely public health responses.Wastewater-based epidemiology(WBE)presents sig...Introduction:Traditional dengue surveillance operates reactively,frequently lagging behind viral transmission patterns and thereby impeding timely public health responses.Wastewater-based epidemiology(WBE)presents significant potential for proactive early warning systems.This study sought to implement and validate the first community-level WBE system for dengue during an active outbreak,evaluating its capacity to detect cryptic transmission and provide actionable intelligence for public health interventions.Methods:During a dengue virus serotype 1(DENV-1)outbreak,we collected 618 wastewater grab samples from manholes within a 200-m radius of 8 reported cases,along with matched patient serum and urine samples.We systematically compared magnetic bead and polyethylene glycol(PEG)concentration methods for viral recovery efficiency.DENV-1 ribonucleic acid(RNA)was detected and quantified using reverse transcription quantitative polymerase chain reaction(RT-qPCR).Positive samples underwent genomic sequencing and phylogenetic analysis to confirm environmental signals and determine viral lineages.Results:The magnetic bead method demonstrated superior performance with a limit of detection of 10 copies/mL and was selected based on its higher recovery efficiency(59.7%).We successfully detected DENV-1 in 14 of 618 wastewater samples tested.Critically,a positive wastewater signal from one residential building preceded the clinical diagnosis of a new case within that same location by several hours.For a single patient,we successfully generated matched viral genomic sequences from serum,urine,and wastewater samples,providing definitive validation of the environmental signal’s authenticity.Conclusions:Community-level wastewater surveillance represents a powerful and effective tool for dengue control programs.This approach provides actionable early warnings by detecting cryptic viral transmission before cases receive clinical identification.Such capabilities enable public health authorities to deploy preemptive,geographically-targeted interventions,including vector control measures,fundamentally improving both the speed and precision of outbreak responses while helping to mitigate disease spread.展开更多
基金supported by the Shenzhen Medical Research Fund(Grant No.A2303049)Guangdong Basic and Applied Basic Research(Grant No.2023A1515010647)+1 种基金National Natural Science Foundation of China(Grant No.22004135)Shenzhen Science and Technology Program(Grant No.RCBS20210706092409020,GXWD20201231165807008,20200824162253002).
文摘Multi-organ-on-a-chip(MOOC)technology represents a pivotal direction in the organ-on-a-chip field,seeking to emulate the complex interactions of multiple human organs in vitro through microfluidic systems.This technology overcomes the limitations of traditional single-organ models,providing a novel platform for investigating complex disease mechanisms and evaluating drug efficacy and toxicity.Although it demonstrates broad application prospects,its development still faces critical bottlenecks,including inadequate physiological coupling between organs,short functional maintenance durations,and limited real-time monitoring capabilities.Contemporary research is advancing along three key directions,including functional coupling,sensor integration,and full-process automation systems,to propel the technology toward enhanced levels of physiological relevance and predictive accuracy.
基金Supported by the National Key R&D Program of China(2024YFC2311500)the Guangdong Medical Science and Technology Research Fund Project(B2025367),the Key Project of Medicine Discipline of Guangzhou(2025–2027-11)the Guangzhou Science and Technology Plan Project(2023A03J0447,2023A03J0454,2023A03J0938,and 2024A03J0369).
文摘Introduction:Traditional dengue surveillance operates reactively,frequently lagging behind viral transmission patterns and thereby impeding timely public health responses.Wastewater-based epidemiology(WBE)presents significant potential for proactive early warning systems.This study sought to implement and validate the first community-level WBE system for dengue during an active outbreak,evaluating its capacity to detect cryptic transmission and provide actionable intelligence for public health interventions.Methods:During a dengue virus serotype 1(DENV-1)outbreak,we collected 618 wastewater grab samples from manholes within a 200-m radius of 8 reported cases,along with matched patient serum and urine samples.We systematically compared magnetic bead and polyethylene glycol(PEG)concentration methods for viral recovery efficiency.DENV-1 ribonucleic acid(RNA)was detected and quantified using reverse transcription quantitative polymerase chain reaction(RT-qPCR).Positive samples underwent genomic sequencing and phylogenetic analysis to confirm environmental signals and determine viral lineages.Results:The magnetic bead method demonstrated superior performance with a limit of detection of 10 copies/mL and was selected based on its higher recovery efficiency(59.7%).We successfully detected DENV-1 in 14 of 618 wastewater samples tested.Critically,a positive wastewater signal from one residential building preceded the clinical diagnosis of a new case within that same location by several hours.For a single patient,we successfully generated matched viral genomic sequences from serum,urine,and wastewater samples,providing definitive validation of the environmental signal’s authenticity.Conclusions:Community-level wastewater surveillance represents a powerful and effective tool for dengue control programs.This approach provides actionable early warnings by detecting cryptic viral transmission before cases receive clinical identification.Such capabilities enable public health authorities to deploy preemptive,geographically-targeted interventions,including vector control measures,fundamentally improving both the speed and precision of outbreak responses while helping to mitigate disease spread.
