从西江流域佛山段水环境保护需求出发,以.NET Framework为系统基础运行环境,使用Google Maps API V3将Google地图嵌入到网页进行标识开发,依托LINQ to SQL技术对基础数据进行对象化操作,通过构建基础数据层、数据存取层、业务逻辑层、U...从西江流域佛山段水环境保护需求出发,以.NET Framework为系统基础运行环境,使用Google Maps API V3将Google地图嵌入到网页进行标识开发,依托LINQ to SQL技术对基础数据进行对象化操作,通过构建基础数据层、数据存取层、业务逻辑层、UI表现层等4层架构,建立环境质量、主要风险源、环境敏感点及环境应急处置资源等信息数据库,运用计算机技术和监测技术,系统实现了水环境质量的可视化实时监控和水质的模拟预测功能,可为流域水环境的风险预警、事故源头追溯和环境污染事故应急提供技术支持。展开更多
Global infectious disease prevention faces escalating challenges due to the continual emergence of novel pathogens and rapid viral mutations.Synthetic biology has revolutionized this field by enabling precise diagnost...Global infectious disease prevention faces escalating challenges due to the continual emergence of novel pathogens and rapid viral mutations.Synthetic biology has revolutionized this field by enabling precise diagnostics,innovative vaccine platforms,and targeted therapeutics,yet it simultaneously raises concerns regarding dual-use potential,biosafety,and ethical governance.This systematic review(2015-2025,PubMed,Web of Science,Scopus)focuses on CRISPR-based diagnostics,synthetic vaccines,and engineered probiotics.CRISPR/Cas systems such as DETECTR(Cas12a)and SHERLOCK(Cas13a)demonstrate high sensitivity and rapid pathogen detection(e.g.,SARS-CoV-2,Ebola),but their misuse could enhance pathogen virulence or enable bioweapon development.mRNA and viral vector vaccines offer flexible and rapid responses to emerging infections but encounter limitations in molecular stability,delivery system toxicity,and ecological safety.Engineered probiotics,designed as“living therapeutics,”can detect pathogens and modulate immune responses,yet pose potential risks of horizontal gene transfer and host-specific variability.Overall,while synthetic biology provides transformative tools for infectious disease control,it necessitates robust global regulatory frameworks,standardized biosafety practices,and ethical oversight to ensure responsible and sustainable application.展开更多
文摘从西江流域佛山段水环境保护需求出发,以.NET Framework为系统基础运行环境,使用Google Maps API V3将Google地图嵌入到网页进行标识开发,依托LINQ to SQL技术对基础数据进行对象化操作,通过构建基础数据层、数据存取层、业务逻辑层、UI表现层等4层架构,建立环境质量、主要风险源、环境敏感点及环境应急处置资源等信息数据库,运用计算机技术和监测技术,系统实现了水环境质量的可视化实时监控和水质的模拟预测功能,可为流域水环境的风险预警、事故源头追溯和环境污染事故应急提供技术支持。
基金supported by Beijing Research Ward Excellence Program(BRWEP2024W102170101)The National Key Research and Development Program(2022YFC2603500,2022YFC2603505)+5 种基金Capital’s Funds for Health Improvement and Research(2022-1-2172)Beijing Municipal Health Commission high-level public health technical personnel construction project(discipline leader-03-26,discipline backbone-02-28)Beijing Hospitals Authority Clinical medicine Development of special funding support(ZLRK202301)Beijing Hospitals Authority“peak”talent training program(DFL20241803)National Key Research and Development Program of China(2023YFC2306900)National Key Research and Development Program of Ministry of Science and Technology(2023YFC2308105).
文摘Global infectious disease prevention faces escalating challenges due to the continual emergence of novel pathogens and rapid viral mutations.Synthetic biology has revolutionized this field by enabling precise diagnostics,innovative vaccine platforms,and targeted therapeutics,yet it simultaneously raises concerns regarding dual-use potential,biosafety,and ethical governance.This systematic review(2015-2025,PubMed,Web of Science,Scopus)focuses on CRISPR-based diagnostics,synthetic vaccines,and engineered probiotics.CRISPR/Cas systems such as DETECTR(Cas12a)and SHERLOCK(Cas13a)demonstrate high sensitivity and rapid pathogen detection(e.g.,SARS-CoV-2,Ebola),but their misuse could enhance pathogen virulence or enable bioweapon development.mRNA and viral vector vaccines offer flexible and rapid responses to emerging infections but encounter limitations in molecular stability,delivery system toxicity,and ecological safety.Engineered probiotics,designed as“living therapeutics,”can detect pathogens and modulate immune responses,yet pose potential risks of horizontal gene transfer and host-specific variability.Overall,while synthetic biology provides transformative tools for infectious disease control,it necessitates robust global regulatory frameworks,standardized biosafety practices,and ethical oversight to ensure responsible and sustainable application.
