Syntaxin 1A(Syx1A)has diverse and indispensable functions in animals.Pre-vious studies have mainly focused on the roles of Syx1A in Drosophila,and so how Syx1A operates during the development of other insects remains ...Syntaxin 1A(Syx1A)has diverse and indispensable functions in animals.Pre-vious studies have mainly focused on the roles of Syx1A in Drosophila,and so how Syx1A operates during the development of other insects remains poorly understood.This study in-vestigated whether disrupting LmSyx1A using RNA interference(RNAi)affects the growth and development of Locusta migratoria.LmSyx1A was expressed in all tissues tested,with the highest expression observed in the fat body.After 5th-instar nymphs were injected with double-stranded LmSyx1A(dsLmSyx1A),none of the nymphs were able to molt normally and all eventually died.The silencing of LmSyx1A resulted in the cessation of feeding,body weight loss,and atrophy of the midgut and gastric cecum in locusts.Hematoxylin and eosin(H&E)staining showed that the columnar cells in the midgut were severely dam-aged,with microvilli defects visible in dsLmSyx1A-injected nymphs.Secretory vesicles were observed with transmission electron microscopy(TEM).In addition,reverse tran-scription quantitative polymerase chain reaction(RT-qPCR)further indicates that silenc-ing LmSyx1A repressed the expression of genes involved in the insulin/mammalian target of rapamycin(mTOR)-associated nutritional pathway.Taken together,these results sug-gest that LmSyx1A significantly affects the midgut cell layer of locust nymphs,which was partially associated with the downregulation of the insulin/mTOR-associated nutritional pathway.Thus,we argue that LmSyx1A is a suitable target for developing dsRNA-based biological pesticides for managing L.migratoria.展开更多
基金supported by the National Key R&D Program of China(2022YFD1700200)the Natural Science Foundation of Shanxi Province,China(20210302123440)the Fund for Shanxi“1331 Project”and the Graduate Education Innovation Project of Shanxi Province,China(2023SJ033).
文摘Syntaxin 1A(Syx1A)has diverse and indispensable functions in animals.Pre-vious studies have mainly focused on the roles of Syx1A in Drosophila,and so how Syx1A operates during the development of other insects remains poorly understood.This study in-vestigated whether disrupting LmSyx1A using RNA interference(RNAi)affects the growth and development of Locusta migratoria.LmSyx1A was expressed in all tissues tested,with the highest expression observed in the fat body.After 5th-instar nymphs were injected with double-stranded LmSyx1A(dsLmSyx1A),none of the nymphs were able to molt normally and all eventually died.The silencing of LmSyx1A resulted in the cessation of feeding,body weight loss,and atrophy of the midgut and gastric cecum in locusts.Hematoxylin and eosin(H&E)staining showed that the columnar cells in the midgut were severely dam-aged,with microvilli defects visible in dsLmSyx1A-injected nymphs.Secretory vesicles were observed with transmission electron microscopy(TEM).In addition,reverse tran-scription quantitative polymerase chain reaction(RT-qPCR)further indicates that silenc-ing LmSyx1A repressed the expression of genes involved in the insulin/mammalian target of rapamycin(mTOR)-associated nutritional pathway.Taken together,these results sug-gest that LmSyx1A significantly affects the midgut cell layer of locust nymphs,which was partially associated with the downregulation of the insulin/mTOR-associated nutritional pathway.Thus,we argue that LmSyx1A is a suitable target for developing dsRNA-based biological pesticides for managing L.migratoria.
