The extensive utilization of the low-energy dipeptide sweetener aspartame in foods leads to various studies on searching for new sweeteners in series. However, the real mechanistic cause of their sweetness power is st...The extensive utilization of the low-energy dipeptide sweetener aspartame in foods leads to various studies on searching for new sweeteners in series. However, the real mechanistic cause of their sweetness power is still not completely known owing to their complex interactions with human sweet receptor, which may be different from that of other sweeteners to some extent. In this contribution, predictive quantitative structure-property relationship(QSPR) models have been developed for diverse aspartame analogues using Materials Studio 5.0 software. The optimal QSPR model(r2 = 0.913, r2 CV = 0.881 and r2 pred = 0.730) constructed by the genetic function approximation method has been validated by the tests of cross validation, randomization, external prediction and other statistical criteria, which shows that their sweetness power is mainly governed by their electrotopological-state indices(SssCH and SsNH), spatial descriptors(Shadow length: LX, ellipsoidal volume and Connolly surface occupied volume) and topological descriptors(Chi(3): cluster and Chi(0)(valence modified)), which partially supports both multipoint attachment theory proposed by Nofre and Tinti et al. and B-X theory proposed by Kier et al.. Present exploited results provide the key structural features for the sweetness power of aspartame analogues, supplement the mechanistic understanding of the sweet perception, and would be also helpful for the design of potent sweetener analogs prior to their synthesis.展开更多
Quantum chemical parameters of 10 amino acids with D- and L-configurations were firstly calculated with semi-empirical AM1 method. Furthermore, the relationship between molecular structures of D-, L-amino acids and t...Quantum chemical parameters of 10 amino acids with D- and L-configurations were firstly calculated with semi-empirical AM1 method. Furthermore, the relationship between molecular structures of D-, L-amino acids and their sweetness were observed. The results show that upon different configurations of amino acids, the sweetness is relative with their formation heat, dipole moment, energy gap of frontier orbital and other parameters. The formation heats of the same amino acids possessing D- and L-configurations are different except glycine. The algebraic value of D-amino acid is generally larger than that of corresponding L-configuration with only one except of tyrosine. The dipole moment of D-amino acid is generally larger than that of corresponding L-amino acid except tyrosine and lysine. The lowest unoccupied orbital energy （ELUMO） of D-amino acid is higher than that of corresponding L-configuration except phenylalanine. △E of D-amino acid is larger than that of L-amino acid except histidine, phenylalanine and lysine. The larger gap will have advantage for its matching with frontier orbital energy of human protein acceptor, which strengthens the interaction between D-amino acid and sweet taste acceptor. Besides, the changing rules of these parameters are generally identical.展开更多
The goals of this study were to examine, using the temporal dominance of sensations (TDS) method, the effects of 1) the sucrose concentration on the temporal sequence of sweetness perception in four fruit-flavored (le...The goals of this study were to examine, using the temporal dominance of sensations (TDS) method, the effects of 1) the sucrose concentration on the temporal sequence of sweetness perception in four fruit-flavored (lemon, peach, strawberry, and vanilla) teas with 0.05, 0.15, and 0.25 M sucrose;and 2) participants’ sex on this temporal sequence. Twenty-four healthy young adults were assigned to female (n = 12) and male (n = 12) groups. Both groups evaluated five sensory attributes in 12 samples (four fruit-flavored teas, each with three sucrose concentrations). As in our preceding study [1], two sensations, sweetness and fruitiness, were dominant after ingesting the 12 teas in both groups, but the temporal sequence of sweetness perception differed between the two groups. On average, the male group reported the first appearance of sweetness earlier in the samples with 0.05 M sucrose compared with the female group, with the samples containing 0.15 and 0.25 M sucrose. The average durations of the sweetness sensation were different between the male and female groups as well as among the sucrose concentrations and the four flavors tested.展开更多
B and C sands of the Lower Goru Formation of Cretaceous are proven reservoirs in different parts of the Middle and Lower Indus Basin,Pakistan.Most of the discoveries in this basin have been made in structural traps.Ho...B and C sands of the Lower Goru Formation of Cretaceous are proven reservoirs in different parts of the Middle and Lower Indus Basin,Pakistan.Most of the discoveries in this basin have been made in structural traps.However,in Sawan gas field;structural inversion,deep burial depth and heterogeneity of reservoir intervals make it difficult to demarcate the sweetness zones through conventional seismic analysis.In this work,different data sets have been integrated through constrained sparse spike inversion to mark sweetness zones in B and C sands of this formation.C sand contains four sweetness zones;the main sweetness zone is located towards the east while three subtle sweetness zones were identified towards the west of Sawan fault.The location of producing and nonproducing wells within the sweetness and outside of sweetness zones confirms the credibility of this work.B sand includes three sweetness zones located towards the west of Sawan fault.Moreover,inverted porosity results not only show good agreement with the porosity log of blind well(Sawan-02)but also show good matching with the core porosities.Hence integration of different data sets leads to demarcate the accurate location,size and extent of the sweetness zones.展开更多
The protein glycosidase APM is a seet agent produced from amino acid necessary to the body. With a sweetness 200 times more than that of the refined sugar, this product has been confirmed as an A (1) Class low heat, h...The protein glycosidase APM is a seet agent produced from amino acid necessary to the body. With a sweetness 200 times more than that of the refined sugar, this product has been confirmed as an A (1) Class low heat, high sweetness agent by specialists of JECFA of the United Nations. A series of toxicology tests undertaken by the United States, China and Japan as well as many other international institutions over past decades, have proven the展开更多
Between the rugged cliffs where the Yarkant River carves through the Taklimakan Desert,a 164.8-meter-tall colossus stands guard-the huge dam of the Altash Water Conservancy Project,the largest water conservancy projec...Between the rugged cliffs where the Yarkant River carves through the Taklimakan Desert,a 164.8-meter-tall colossus stands guard-the huge dam of the Altash Water Conservancy Project,the largest water conservancy project in Xinjiang Uygur Autonomous Region.展开更多
An influx of small investors eager to earn money puts heavy pressure on fund managers Fund managers control millions in capital and call the shots in the stock market. They can make a fortune from a single judgment. T...An influx of small investors eager to earn money puts heavy pressure on fund managers Fund managers control millions in capital and call the shots in the stock market. They can make a fortune from a single judgment. The debut of fund management展开更多
The town of Pangezhuang in suburban Beijing,also known as the land of watermelons,has been hosting a watermelon festival for 32 years to celebrate its green prowess.And among the watermelon growers in the town,Song Sh...The town of Pangezhuang in suburban Beijing,also known as the land of watermelons,has been hosting a watermelon festival for 32 years to celebrate its green prowess.And among the watermelon growers in the town,Song Shaotang is one of the most famous ones,a second-generation entrant who learned the skills from his father,a melon grower since the 1980s.展开更多
This year's Mid-Autumn Festival fell on Sept.10,the sameday as Teachers'Day.People were glad to see many fun activities that celebrated the traditional estival and showed their gratitude for teachers.
SWEET(sugars will eventually be exported transporters)蛋白是近年来鉴定的新型糖转运蛋白,在植物生长发育和果实品质形成过程中有重要作用。为探究金柑SWEET基因家族特点及其在果实发育过程中的表达,从金柑基因组数据库中鉴定出17个...SWEET(sugars will eventually be exported transporters)蛋白是近年来鉴定的新型糖转运蛋白,在植物生长发育和果实品质形成过程中有重要作用。为探究金柑SWEET基因家族特点及其在果实发育过程中的表达,从金柑基因组数据库中鉴定出17个SWEET成员,生物信息学分析表明金柑SWEET基因家族多数成员具有典型的7次跨膜螺旋结构,属于疏水碱性蛋白,在基序和结构域上相对保守。进化分析表明金柑SWEET成员可以分为4类。蛋白质互作网络分析发现FhSWEET16b是重要的核心家族成员。基因表达分析发现FhSWEET1、FhSWEET2b、FhSWEET15和FhSWEET16a在果实发育过程中表达量较高,可能在金柑果实糖分积累中起关键作用。本研究结果为进一步分析柑橘SWEET基因调控果实糖积累的分子机制提供基础。展开更多
Gas-bearing shales have become a major source of future natural gas production worldwide.It has become increasingly urgent to develop a reliable prediction model and corresponding workflow for identifying shale gas sw...Gas-bearing shales have become a major source of future natural gas production worldwide.It has become increasingly urgent to develop a reliable prediction model and corresponding workflow for identifying shale gas sweet spots.The formation of gas-bearing shales is closely linked to relative sealevel changes,providing an important approach to predicting sweet spots in the Wufeng-Longmaxi shale in the southern Sichuan Basin,China.Three types of marine shale gas sweet spots are identified in the shale based on their formation stages combined with relative sea-level changes:early,middle,and late transgression types.This study develops a prediction model and workflow for identifying shale gas sweet spots by analyzing relative sea-level changes and facies sequences.Predicting shale gas sweet spots in an explored block using this model and workflow can provide a valuable guide for well design and hydraulic fracturing,significantly enhancing the efficiency of shale gas exploration and development.Notably,the new prediction model and workflow can be utilized for the rapid evaluation of the potential for shale gas development in new shale gas blocks or those with low exploratory maturity.展开更多
目的分析尼拉帕利所致药物不良反应(ADR)的发生情况及其临床特点。方法检索中国知网、万方、维普、PubMed、Web of Science数据库中收录的尼拉帕利致ADR个案报道,并对ADR的类型、发生时间、合并用药情况进行分析。结果共筛选出有效文献8...目的分析尼拉帕利所致药物不良反应(ADR)的发生情况及其临床特点。方法检索中国知网、万方、维普、PubMed、Web of Science数据库中收录的尼拉帕利致ADR个案报道,并对ADR的类型、发生时间、合并用药情况进行分析。结果共筛选出有效文献8篇,涉及研究对象共计13例。13例患者年龄43~82岁。其中3例患者合并使用其他药物,其他患者不详。12例患者用药剂量在常规范围内,1例患者的相关信息缺失。在不良反应发生时间已知的患者中,多数患者在用药后1个月内出现ADR。13例患者中,11例患者出现血液系统疾病(10例为血小板减少,1例为全血细胞减少),另有1例出现皮肤Sweet综合征,1例出现间质性肺病。结论尼拉帕利引起的ADR需引起临床高度重视,应加强用药监测,以减少潜在致命性ADR的发生。展开更多
Increasing access to locally produced, safe, nutritious and affordable complementary foods is essential to combat micronutrient deficiencies in young children in low- and middle-income countries. Two formulations of s...Increasing access to locally produced, safe, nutritious and affordable complementary foods is essential to combat micronutrient deficiencies in young children in low- and middle-income countries. Two formulations of sweets based on jujube, baobab fruit powder and sweet potato puree were produced, and their nutritional values and sensory properties such as taste, smell, color and acceptability were assessed. The formulation containing the most jujube powder, baobab fruit and sweet potato puree gave the best nutritional value. The sweets are rich in calcium (259.80 mg/100 g), magnesium (67.98 mg/100 g), potassium (782.04 mg/100 g), vitamin C (32.37 mg/100 g), iron (6.45 g mg/100 g) and zinc (0.97 mg/100 g). Sensory tests on the two formulations showed acceptability scores ranging from 81.26% to 84.58%, well above the minimum acceptable threshold. Candies with jujube, baobab fruit powder and sweet potato puree could be an alternative for preventing micronutrient deficiencies.展开更多
Sweet cherry(Prunus avium)represents a significant stone fruit crop in temperate regions worldwide.While molecular breeding has progressed substantially following the initial sweet cherry genome release,existing genom...Sweet cherry(Prunus avium)represents a significant stone fruit crop in temperate regions worldwide.While molecular breeding has progressed substantially following the initial sweet cherry genome release,existing genome assemblies contain unresolved gaps and comprise consensus chimeric sequences that fail to differentiate haplotype alleles,significantly constraining research on important agronomic trait inheritance.This study presents a phased-resolved telomere-to-telomere reference genome of sweet cherry‘Tieton’.The assembly anchors 653.03 Mb of sequence onto 16 pseudochromosomes representing two haplotypes,with 67,012 coding genes identified(33,777 in hapA and 33,235 in hapB).The genome demonstrates superior quality metrics,including a consensus accuracy exceeding QV44,contig N50 above 17.94 Mb,Benchmarking Universal Single-Copy Orthologs completeness of 98.7%,and a long terminal repeat(LTR)assembly index exceeding 20.This genome provides phased and annotated chromosome pairs,offering a comprehensive view of sweet cherry’s diploid genome organization.Utilizing this reference genome,we identified a large fragment deletion associated with yellow-skinned fruit in sweet cherry‘13-33’.This resource will significantly enhance breeding efforts and genetic research in sweet cherries.展开更多
基金Supported by the National Natural Science Foundation of China(No.21673207)Special Fundamental Research Fund for the Central Public Scientific Research Institutes(No.562018Y-5983)Zhejiang Provincial Collaborative Innovation Center of Food Safety and Nutrition(No.2017SICR115,2017SICR101)
文摘The extensive utilization of the low-energy dipeptide sweetener aspartame in foods leads to various studies on searching for new sweeteners in series. However, the real mechanistic cause of their sweetness power is still not completely known owing to their complex interactions with human sweet receptor, which may be different from that of other sweeteners to some extent. In this contribution, predictive quantitative structure-property relationship(QSPR) models have been developed for diverse aspartame analogues using Materials Studio 5.0 software. The optimal QSPR model(r2 = 0.913, r2 CV = 0.881 and r2 pred = 0.730) constructed by the genetic function approximation method has been validated by the tests of cross validation, randomization, external prediction and other statistical criteria, which shows that their sweetness power is mainly governed by their electrotopological-state indices(SssCH and SsNH), spatial descriptors(Shadow length: LX, ellipsoidal volume and Connolly surface occupied volume) and topological descriptors(Chi(3): cluster and Chi(0)(valence modified)), which partially supports both multipoint attachment theory proposed by Nofre and Tinti et al. and B-X theory proposed by Kier et al.. Present exploited results provide the key structural features for the sweetness power of aspartame analogues, supplement the mechanistic understanding of the sweet perception, and would be also helpful for the design of potent sweetener analogs prior to their synthesis.
文摘Quantum chemical parameters of 10 amino acids with D- and L-configurations were firstly calculated with semi-empirical AM1 method. Furthermore, the relationship between molecular structures of D-, L-amino acids and their sweetness were observed. The results show that upon different configurations of amino acids, the sweetness is relative with their formation heat, dipole moment, energy gap of frontier orbital and other parameters. The formation heats of the same amino acids possessing D- and L-configurations are different except glycine. The algebraic value of D-amino acid is generally larger than that of corresponding L-configuration with only one except of tyrosine. The dipole moment of D-amino acid is generally larger than that of corresponding L-amino acid except tyrosine and lysine. The lowest unoccupied orbital energy （ELUMO） of D-amino acid is higher than that of corresponding L-configuration except phenylalanine. △E of D-amino acid is larger than that of L-amino acid except histidine, phenylalanine and lysine. The larger gap will have advantage for its matching with frontier orbital energy of human protein acceptor, which strengthens the interaction between D-amino acid and sweet taste acceptor. Besides, the changing rules of these parameters are generally identical.
文摘The goals of this study were to examine, using the temporal dominance of sensations (TDS) method, the effects of 1) the sucrose concentration on the temporal sequence of sweetness perception in four fruit-flavored (lemon, peach, strawberry, and vanilla) teas with 0.05, 0.15, and 0.25 M sucrose;and 2) participants’ sex on this temporal sequence. Twenty-four healthy young adults were assigned to female (n = 12) and male (n = 12) groups. Both groups evaluated five sensory attributes in 12 samples (four fruit-flavored teas, each with three sucrose concentrations). As in our preceding study [1], two sensations, sweetness and fruitiness, were dominant after ingesting the 12 teas in both groups, but the temporal sequence of sweetness perception differed between the two groups. On average, the male group reported the first appearance of sweetness earlier in the samples with 0.05 M sucrose compared with the female group, with the samples containing 0.15 and 0.25 M sucrose. The average durations of the sweetness sensation were different between the male and female groups as well as among the sucrose concentrations and the four flavors tested.
文摘B and C sands of the Lower Goru Formation of Cretaceous are proven reservoirs in different parts of the Middle and Lower Indus Basin,Pakistan.Most of the discoveries in this basin have been made in structural traps.However,in Sawan gas field;structural inversion,deep burial depth and heterogeneity of reservoir intervals make it difficult to demarcate the sweetness zones through conventional seismic analysis.In this work,different data sets have been integrated through constrained sparse spike inversion to mark sweetness zones in B and C sands of this formation.C sand contains four sweetness zones;the main sweetness zone is located towards the east while three subtle sweetness zones were identified towards the west of Sawan fault.The location of producing and nonproducing wells within the sweetness and outside of sweetness zones confirms the credibility of this work.B sand includes three sweetness zones located towards the west of Sawan fault.Moreover,inverted porosity results not only show good agreement with the porosity log of blind well(Sawan-02)but also show good matching with the core porosities.Hence integration of different data sets leads to demarcate the accurate location,size and extent of the sweetness zones.
文摘The protein glycosidase APM is a seet agent produced from amino acid necessary to the body. With a sweetness 200 times more than that of the refined sugar, this product has been confirmed as an A (1) Class low heat, high sweetness agent by specialists of JECFA of the United Nations. A series of toxicology tests undertaken by the United States, China and Japan as well as many other international institutions over past decades, have proven the
文摘Between the rugged cliffs where the Yarkant River carves through the Taklimakan Desert,a 164.8-meter-tall colossus stands guard-the huge dam of the Altash Water Conservancy Project,the largest water conservancy project in Xinjiang Uygur Autonomous Region.
文摘An influx of small investors eager to earn money puts heavy pressure on fund managers Fund managers control millions in capital and call the shots in the stock market. They can make a fortune from a single judgment. The debut of fund management
文摘The town of Pangezhuang in suburban Beijing,also known as the land of watermelons,has been hosting a watermelon festival for 32 years to celebrate its green prowess.And among the watermelon growers in the town,Song Shaotang is one of the most famous ones,a second-generation entrant who learned the skills from his father,a melon grower since the 1980s.
文摘This year's Mid-Autumn Festival fell on Sept.10,the sameday as Teachers'Day.People were glad to see many fun activities that celebrated the traditional estival and showed their gratitude for teachers.
文摘SWEET(sugars will eventually be exported transporters)蛋白是近年来鉴定的新型糖转运蛋白,在植物生长发育和果实品质形成过程中有重要作用。为探究金柑SWEET基因家族特点及其在果实发育过程中的表达,从金柑基因组数据库中鉴定出17个SWEET成员,生物信息学分析表明金柑SWEET基因家族多数成员具有典型的7次跨膜螺旋结构,属于疏水碱性蛋白,在基序和结构域上相对保守。进化分析表明金柑SWEET成员可以分为4类。蛋白质互作网络分析发现FhSWEET16b是重要的核心家族成员。基因表达分析发现FhSWEET1、FhSWEET2b、FhSWEET15和FhSWEET16a在果实发育过程中表达量较高,可能在金柑果实糖分积累中起关键作用。本研究结果为进一步分析柑橘SWEET基因调控果实糖积累的分子机制提供基础。
文摘Gas-bearing shales have become a major source of future natural gas production worldwide.It has become increasingly urgent to develop a reliable prediction model and corresponding workflow for identifying shale gas sweet spots.The formation of gas-bearing shales is closely linked to relative sealevel changes,providing an important approach to predicting sweet spots in the Wufeng-Longmaxi shale in the southern Sichuan Basin,China.Three types of marine shale gas sweet spots are identified in the shale based on their formation stages combined with relative sea-level changes:early,middle,and late transgression types.This study develops a prediction model and workflow for identifying shale gas sweet spots by analyzing relative sea-level changes and facies sequences.Predicting shale gas sweet spots in an explored block using this model and workflow can provide a valuable guide for well design and hydraulic fracturing,significantly enhancing the efficiency of shale gas exploration and development.Notably,the new prediction model and workflow can be utilized for the rapid evaluation of the potential for shale gas development in new shale gas blocks or those with low exploratory maturity.
文摘目的分析尼拉帕利所致药物不良反应(ADR)的发生情况及其临床特点。方法检索中国知网、万方、维普、PubMed、Web of Science数据库中收录的尼拉帕利致ADR个案报道,并对ADR的类型、发生时间、合并用药情况进行分析。结果共筛选出有效文献8篇,涉及研究对象共计13例。13例患者年龄43~82岁。其中3例患者合并使用其他药物,其他患者不详。12例患者用药剂量在常规范围内,1例患者的相关信息缺失。在不良反应发生时间已知的患者中,多数患者在用药后1个月内出现ADR。13例患者中,11例患者出现血液系统疾病(10例为血小板减少,1例为全血细胞减少),另有1例出现皮肤Sweet综合征,1例出现间质性肺病。结论尼拉帕利引起的ADR需引起临床高度重视,应加强用药监测,以减少潜在致命性ADR的发生。
文摘Increasing access to locally produced, safe, nutritious and affordable complementary foods is essential to combat micronutrient deficiencies in young children in low- and middle-income countries. Two formulations of sweets based on jujube, baobab fruit powder and sweet potato puree were produced, and their nutritional values and sensory properties such as taste, smell, color and acceptability were assessed. The formulation containing the most jujube powder, baobab fruit and sweet potato puree gave the best nutritional value. The sweets are rich in calcium (259.80 mg/100 g), magnesium (67.98 mg/100 g), potassium (782.04 mg/100 g), vitamin C (32.37 mg/100 g), iron (6.45 g mg/100 g) and zinc (0.97 mg/100 g). Sensory tests on the two formulations showed acceptability scores ranging from 81.26% to 84.58%, well above the minimum acceptable threshold. Candies with jujube, baobab fruit powder and sweet potato puree could be an alternative for preventing micronutrient deficiencies.
基金supported by the Shandong Provincial Natural Science Foundation,China(ZR2021MC117)the Key R&D Program of Shandong Province,China(2022TZXD006).
文摘Sweet cherry(Prunus avium)represents a significant stone fruit crop in temperate regions worldwide.While molecular breeding has progressed substantially following the initial sweet cherry genome release,existing genome assemblies contain unresolved gaps and comprise consensus chimeric sequences that fail to differentiate haplotype alleles,significantly constraining research on important agronomic trait inheritance.This study presents a phased-resolved telomere-to-telomere reference genome of sweet cherry‘Tieton’.The assembly anchors 653.03 Mb of sequence onto 16 pseudochromosomes representing two haplotypes,with 67,012 coding genes identified(33,777 in hapA and 33,235 in hapB).The genome demonstrates superior quality metrics,including a consensus accuracy exceeding QV44,contig N50 above 17.94 Mb,Benchmarking Universal Single-Copy Orthologs completeness of 98.7%,and a long terminal repeat(LTR)assembly index exceeding 20.This genome provides phased and annotated chromosome pairs,offering a comprehensive view of sweet cherry’s diploid genome organization.Utilizing this reference genome,we identified a large fragment deletion associated with yellow-skinned fruit in sweet cherry‘13-33’.This resource will significantly enhance breeding efforts and genetic research in sweet cherries.
