Anthocyanins play a crucial role in shaping the visual appeal and nutritional quality of fruits.Previous research on anthocyanin biosynthesis in sweet cherry(Prunus avium L.)has primarily relied on single-omics approa...Anthocyanins play a crucial role in shaping the visual appeal and nutritional quality of fruits.Previous research on anthocyanin biosynthesis in sweet cherry(Prunus avium L.)has primarily relied on single-omics approaches or focused on a limited range of metabolites,leaving the regulatory mechanisms and dynamic metabolism of anthocyanins during ripening inadequately characterized.This study integrated anthocyanin-targeted metabolomics and transcriptomics to identify key anthocyanins in sweet cherry and construct a transcriptional regulatory network for anthocyanin biosynthesis.A novel bHLH transcription factor,Prunus avium bHLH transcription factor 102(PavbHLH102),was identified,and its role in regulating cyanidin levels was validated through overexpression and silencing experiments.Both in vitro and in vivo assays demonstrated that PavbHLH102 activates key anthocyanin biosynthetic genes,including PavF3H,PavDFR,and PavUFGT,thereby enhancing fruit coloration.Notably,PavF3′H upregulation significantly increased cyanidin accumulation.This study provides new insights into anthocyanin regulation in sweet cherry and offers valuable resources for improving fruit quality.展开更多
Increasing access to locally produced, safe, nutritious and affordable complementary foods is essential to combat micronutrient deficiencies in young children in low- and middle-income countries. Two formulations of s...Increasing access to locally produced, safe, nutritious and affordable complementary foods is essential to combat micronutrient deficiencies in young children in low- and middle-income countries. Two formulations of sweets based on jujube, baobab fruit powder and sweet potato puree were produced, and their nutritional values and sensory properties such as taste, smell, color and acceptability were assessed. The formulation containing the most jujube powder, baobab fruit and sweet potato puree gave the best nutritional value. The sweets are rich in calcium (259.80 mg/100 g), magnesium (67.98 mg/100 g), potassium (782.04 mg/100 g), vitamin C (32.37 mg/100 g), iron (6.45 g mg/100 g) and zinc (0.97 mg/100 g). Sensory tests on the two formulations showed acceptability scores ranging from 81.26% to 84.58%, well above the minimum acceptable threshold. Candies with jujube, baobab fruit powder and sweet potato puree could be an alternative for preventing micronutrient deficiencies.展开更多
SWEET(sugars will eventually be exported transporters)蛋白是近年来鉴定的新型糖转运蛋白,在植物生长发育和果实品质形成过程中有重要作用。为探究金柑SWEET基因家族特点及其在果实发育过程中的表达,从金柑基因组数据库中鉴定出17个...SWEET(sugars will eventually be exported transporters)蛋白是近年来鉴定的新型糖转运蛋白,在植物生长发育和果实品质形成过程中有重要作用。为探究金柑SWEET基因家族特点及其在果实发育过程中的表达,从金柑基因组数据库中鉴定出17个SWEET成员,生物信息学分析表明金柑SWEET基因家族多数成员具有典型的7次跨膜螺旋结构,属于疏水碱性蛋白,在基序和结构域上相对保守。进化分析表明金柑SWEET成员可以分为4类。蛋白质互作网络分析发现FhSWEET16b是重要的核心家族成员。基因表达分析发现FhSWEET1、FhSWEET2b、FhSWEET15和FhSWEET16a在果实发育过程中表达量较高,可能在金柑果实糖分积累中起关键作用。本研究结果为进一步分析柑橘SWEET基因调控果实糖积累的分子机制提供基础。展开更多
Bud endodormancy represents an ecological adaptation mechanism in perennial deciduous fruit trees to endure winter cold conditions.Sucrose serves a crucial role in bud endodormancy as both an energy metabolizer and si...Bud endodormancy represents an ecological adaptation mechanism in perennial deciduous fruit trees to endure winter cold conditions.Sucrose serves a crucial role in bud endodormancy as both an energy metabolizer and signaling molecule.Sugars Will Eventually be Exported Transporters(SWEETs)function as sugar-efflux transporters that respond to environmental stimuli and contribute to plant growth and development.While SWEET gene families have been identified in various plant species for sugar transport regulation,their mechanism in regulating peach bud endodormancy remains undefined.In this study,we identified 15 SWEET genes in peach.The nomenclature was established through homologous alignment with the Arabidopsis SWEET gene family,resulting in four distinct clades through phylogenetic analysis.Covariance correlation analysis revealed 6 and 12 collinear SWEET genes in peach and Arabidopsis,respectively,forming 13 collinear gene pairs.Real-time quantitative polymerase chain reaction(RT-qPCR)analysis demonstrated significantly elevated expression of PpSWEET6 during peach bud endodormancy release,correlating positively with sucrose content.Transient overexpression of PpSWEET6 enhanced peach bud endodormancy release,while overexpressing PpSWEET6 in Arabidopsis enhanced seed germination and flowering.Y2H and luciferase complementation imaging(LCI)assays confirmed PpSWEET6 interacted with PpABF2.Additionally,dual luciferase reporter(DLR)assays showed that PpSWEET6 significantly decreased the activation of PpDAM6(key dormancy-inducing gene)through PpABF2,thereby modulating peach bud endodormancy release.These findings advance our understanding of SWEET genes in peach bud endodormancy regulation.展开更多
Gas-bearing shales have become a major source of future natural gas production worldwide.It has become increasingly urgent to develop a reliable prediction model and corresponding workflow for identifying shale gas sw...Gas-bearing shales have become a major source of future natural gas production worldwide.It has become increasingly urgent to develop a reliable prediction model and corresponding workflow for identifying shale gas sweet spots.The formation of gas-bearing shales is closely linked to relative sealevel changes,providing an important approach to predicting sweet spots in the Wufeng-Longmaxi shale in the southern Sichuan Basin,China.Three types of marine shale gas sweet spots are identified in the shale based on their formation stages combined with relative sea-level changes:early,middle,and late transgression types.This study develops a prediction model and workflow for identifying shale gas sweet spots by analyzing relative sea-level changes and facies sequences.Predicting shale gas sweet spots in an explored block using this model and workflow can provide a valuable guide for well design and hydraulic fracturing,significantly enhancing the efficiency of shale gas exploration and development.Notably,the new prediction model and workflow can be utilized for the rapid evaluation of the potential for shale gas development in new shale gas blocks or those with low exploratory maturity.展开更多
Sweet potato is one of the most important crops in Côte d’Ivoire. However, some constraints related to climate change limit its production. This study evaluated the agronomic and technological performances of ei...Sweet potato is one of the most important crops in Côte d’Ivoire. However, some constraints related to climate change limit its production. This study evaluated the agronomic and technological performances of eight varieties of orange-fleshed of sweet potato (Ipomoea batatas (L.) to identify the most promising ones. The results show three groups of varieties with different agronomic performances. Group 1 composed of Irene and Vita is characterized by low production of small and large tuberous roots, low number of productive plants and low yield. Group 2 consisting mainly of Kabode is characterized by high production and weight of large tuberous roots as well as high yield. Group 3, composed of Tacha-2 Ininda, Ejumula Gloria, TIB-440060, CIP-199062-1, Bela bela is characterized by high production of small and large tuberous roots, high number of productive plants and average yield. The TIB-440060 variety has a recovery rate of 100%. The Irene, Kabode, Vita, Kabode varieties were resistant to viral and fungal diseases. In terms of technological quality, the TIB-440060 and CIP-199062-1 varieties have the best organoleptic qualities. Given the agronomic performances of the Kabode, TIB-440060 and CIP-199062-1 varieties, could be selected as the elites varieties and distributed to producers.展开更多
Sweet cherry(Prunus avium)represents a significant stone fruit crop in temperate regions worldwide.While molecular breeding has progressed substantially following the initial sweet cherry genome release,existing genom...Sweet cherry(Prunus avium)represents a significant stone fruit crop in temperate regions worldwide.While molecular breeding has progressed substantially following the initial sweet cherry genome release,existing genome assemblies contain unresolved gaps and comprise consensus chimeric sequences that fail to differentiate haplotype alleles,significantly constraining research on important agronomic trait inheritance.This study presents a phased-resolved telomere-to-telomere reference genome of sweet cherry‘Tieton’.The assembly anchors 653.03 Mb of sequence onto 16 pseudochromosomes representing two haplotypes,with 67,012 coding genes identified(33,777 in hapA and 33,235 in hapB).The genome demonstrates superior quality metrics,including a consensus accuracy exceeding QV44,contig N50 above 17.94 Mb,Benchmarking Universal Single-Copy Orthologs completeness of 98.7%,and a long terminal repeat(LTR)assembly index exceeding 20.This genome provides phased and annotated chromosome pairs,offering a comprehensive view of sweet cherry’s diploid genome organization.Utilizing this reference genome,we identified a large fragment deletion associated with yellow-skinned fruit in sweet cherry‘13-33’.This resource will significantly enhance breeding efforts and genetic research in sweet cherries.展开更多
Conversation A(in the break room)Sara:Do I see a picture of a pie?Greg:Youdo.I'm looking at achart of people'sfavoritefall sweettreats.Sara:Yummy!Applepie ismyfavorite.Greg:It'salsothefavoriteof 30percento...Conversation A(in the break room)Sara:Do I see a picture of a pie?Greg:Youdo.I'm looking at achart of people'sfavoritefall sweettreats.Sara:Yummy!Applepie ismyfavorite.Greg:It'salsothefavoriteof 30percentofpeople.This chartsays that.Sara:Well,apple pie is great.展开更多
Located in the heart of southern China’s Jiangnan region,Wuxi is a famous city rich in history and culture during the Spring Festival,showing a unique charm.One of the most interesting features of Wuxi’s New Year ce...Located in the heart of southern China’s Jiangnan region,Wuxi is a famous city rich in history and culture during the Spring Festival,showing a unique charm.One of the most interesting features of Wuxi’s New Year celebrations lies in its sweet taste.Unlike many other parts of China where spicy(辛辣)or savory delicacies dominate the feast,Wuxi boasts a tradition of sweet foods during the Spring Festival.展开更多
Sweet potato varieties exhibited distinct feedback mechanisms in response to continuous cropping obstacles(CCO).This study evaluated the tolerance to CCO(TCCO)among three types(fresh,purple,and starch),each comprising...Sweet potato varieties exhibited distinct feedback mechanisms in response to continuous cropping obstacles(CCO).This study evaluated the tolerance to CCO(TCCO)among three types(fresh,purple,and starch),each comprising five varieties,cultivated in a 16-year CCO plot(CCp)and adjacent non-continuous cropping plots(NCCp)in China.Yield,resistance coefficient(k_(Y),yield ratio between CCp and NCCp),and nitrogen(N),phosphorus(P),and potassium(K)contents and relative accumulation(k_(N),k_(P),k_(K),similar to k_(Y))in chunks or stem vines,were analyzed.Significant differences(p<0.05)in yield and nutrient contents were observed among all varieties and types.Four varieties(purple:Xu A1-144,Xu D9-123,and starch:Shang 19,Zhe 13)exhibited k_(Y)>1.0,indicating higher yields under CCp.Nutrient imbalance-particularly enhanced N uptake,was associated with CCO susceptibility.Fresh and purple chunks preferentially accumulated N,P and K,respectively,while starch varieties plants strongly absorbed more K.Under NCCp,chunks nutrient levels were correlated with multiple elements in stem vines.Under CCp,each chunk nutrient was primarily affected only by its homologous elements in stem vines.Notably,stem vine k_(K) positively correlated with yield under CCO(r=0.34,p<0.05),and stem vines k_(N) significantly correlated with both chunks k_(N) and stem vines k_(K)(p<0.05).Starch sweet potatoes demonstrated the most balanced NPK absorption for TCCO,with yield and nutrient absorption advantages.TCCO was closely linked to efficient and coordinated N-K absorption,regulated by genetic traits and soil nutrient status.Imbalanced NPK ratios and hindered K absorption played a central role in CCO.Strategies focusing on K management and breeding varieties with inter-organ nutrient coordination abilities could enhance the stress resistance of sweet potato production systems.These findings provide genetic resources and insight into the mechanism of CCO tolerance in sweet potato.展开更多
基金funding from the Natural Science Foundation of Sichuan Province,China(Grant Nos.2025ZNSFSC1100,2024NSFSC0324)the National Key Research and Development Project(Grant No.2017YFC0505104)+2 种基金the Sichuan Agricultural University Dual Support Plan Special Project(Grant No.2024ZYTS020)the Postdoctoral Fellowship Program(Grade B)of China Postdoctoral Science Foundation(Grant No.GZB20250863)the China Postdoctoral Science Foundation(Grant No.2025M773740).
文摘Anthocyanins play a crucial role in shaping the visual appeal and nutritional quality of fruits.Previous research on anthocyanin biosynthesis in sweet cherry(Prunus avium L.)has primarily relied on single-omics approaches or focused on a limited range of metabolites,leaving the regulatory mechanisms and dynamic metabolism of anthocyanins during ripening inadequately characterized.This study integrated anthocyanin-targeted metabolomics and transcriptomics to identify key anthocyanins in sweet cherry and construct a transcriptional regulatory network for anthocyanin biosynthesis.A novel bHLH transcription factor,Prunus avium bHLH transcription factor 102(PavbHLH102),was identified,and its role in regulating cyanidin levels was validated through overexpression and silencing experiments.Both in vitro and in vivo assays demonstrated that PavbHLH102 activates key anthocyanin biosynthetic genes,including PavF3H,PavDFR,and PavUFGT,thereby enhancing fruit coloration.Notably,PavF3′H upregulation significantly increased cyanidin accumulation.This study provides new insights into anthocyanin regulation in sweet cherry and offers valuable resources for improving fruit quality.
文摘Increasing access to locally produced, safe, nutritious and affordable complementary foods is essential to combat micronutrient deficiencies in young children in low- and middle-income countries. Two formulations of sweets based on jujube, baobab fruit powder and sweet potato puree were produced, and their nutritional values and sensory properties such as taste, smell, color and acceptability were assessed. The formulation containing the most jujube powder, baobab fruit and sweet potato puree gave the best nutritional value. The sweets are rich in calcium (259.80 mg/100 g), magnesium (67.98 mg/100 g), potassium (782.04 mg/100 g), vitamin C (32.37 mg/100 g), iron (6.45 g mg/100 g) and zinc (0.97 mg/100 g). Sensory tests on the two formulations showed acceptability scores ranging from 81.26% to 84.58%, well above the minimum acceptable threshold. Candies with jujube, baobab fruit powder and sweet potato puree could be an alternative for preventing micronutrient deficiencies.
文摘SWEET(sugars will eventually be exported transporters)蛋白是近年来鉴定的新型糖转运蛋白,在植物生长发育和果实品质形成过程中有重要作用。为探究金柑SWEET基因家族特点及其在果实发育过程中的表达,从金柑基因组数据库中鉴定出17个SWEET成员,生物信息学分析表明金柑SWEET基因家族多数成员具有典型的7次跨膜螺旋结构,属于疏水碱性蛋白,在基序和结构域上相对保守。进化分析表明金柑SWEET成员可以分为4类。蛋白质互作网络分析发现FhSWEET16b是重要的核心家族成员。基因表达分析发现FhSWEET1、FhSWEET2b、FhSWEET15和FhSWEET16a在果实发育过程中表达量较高,可能在金柑果实糖分积累中起关键作用。本研究结果为进一步分析柑橘SWEET基因调控果实糖积累的分子机制提供基础。
基金funded by the National Key Research and Development Program of China(2018YFD1000104)the Shandong Provincial Fruit Industry Technology System Cultivation and Soil Fertilization Post,China(SDAIT-06-04)the Key R&D Program of Shandong Province(Competitive Innovation Platform),China(2022CXPT017).
文摘Bud endodormancy represents an ecological adaptation mechanism in perennial deciduous fruit trees to endure winter cold conditions.Sucrose serves a crucial role in bud endodormancy as both an energy metabolizer and signaling molecule.Sugars Will Eventually be Exported Transporters(SWEETs)function as sugar-efflux transporters that respond to environmental stimuli and contribute to plant growth and development.While SWEET gene families have been identified in various plant species for sugar transport regulation,their mechanism in regulating peach bud endodormancy remains undefined.In this study,we identified 15 SWEET genes in peach.The nomenclature was established through homologous alignment with the Arabidopsis SWEET gene family,resulting in four distinct clades through phylogenetic analysis.Covariance correlation analysis revealed 6 and 12 collinear SWEET genes in peach and Arabidopsis,respectively,forming 13 collinear gene pairs.Real-time quantitative polymerase chain reaction(RT-qPCR)analysis demonstrated significantly elevated expression of PpSWEET6 during peach bud endodormancy release,correlating positively with sucrose content.Transient overexpression of PpSWEET6 enhanced peach bud endodormancy release,while overexpressing PpSWEET6 in Arabidopsis enhanced seed germination and flowering.Y2H and luciferase complementation imaging(LCI)assays confirmed PpSWEET6 interacted with PpABF2.Additionally,dual luciferase reporter(DLR)assays showed that PpSWEET6 significantly decreased the activation of PpDAM6(key dormancy-inducing gene)through PpABF2,thereby modulating peach bud endodormancy release.These findings advance our understanding of SWEET genes in peach bud endodormancy regulation.
文摘Gas-bearing shales have become a major source of future natural gas production worldwide.It has become increasingly urgent to develop a reliable prediction model and corresponding workflow for identifying shale gas sweet spots.The formation of gas-bearing shales is closely linked to relative sealevel changes,providing an important approach to predicting sweet spots in the Wufeng-Longmaxi shale in the southern Sichuan Basin,China.Three types of marine shale gas sweet spots are identified in the shale based on their formation stages combined with relative sea-level changes:early,middle,and late transgression types.This study develops a prediction model and workflow for identifying shale gas sweet spots by analyzing relative sea-level changes and facies sequences.Predicting shale gas sweet spots in an explored block using this model and workflow can provide a valuable guide for well design and hydraulic fracturing,significantly enhancing the efficiency of shale gas exploration and development.Notably,the new prediction model and workflow can be utilized for the rapid evaluation of the potential for shale gas development in new shale gas blocks or those with low exploratory maturity.
文摘Sweet potato is one of the most important crops in Côte d’Ivoire. However, some constraints related to climate change limit its production. This study evaluated the agronomic and technological performances of eight varieties of orange-fleshed of sweet potato (Ipomoea batatas (L.) to identify the most promising ones. The results show three groups of varieties with different agronomic performances. Group 1 composed of Irene and Vita is characterized by low production of small and large tuberous roots, low number of productive plants and low yield. Group 2 consisting mainly of Kabode is characterized by high production and weight of large tuberous roots as well as high yield. Group 3, composed of Tacha-2 Ininda, Ejumula Gloria, TIB-440060, CIP-199062-1, Bela bela is characterized by high production of small and large tuberous roots, high number of productive plants and average yield. The TIB-440060 variety has a recovery rate of 100%. The Irene, Kabode, Vita, Kabode varieties were resistant to viral and fungal diseases. In terms of technological quality, the TIB-440060 and CIP-199062-1 varieties have the best organoleptic qualities. Given the agronomic performances of the Kabode, TIB-440060 and CIP-199062-1 varieties, could be selected as the elites varieties and distributed to producers.
基金supported by the Shandong Provincial Natural Science Foundation,China(ZR2021MC117)the Key R&D Program of Shandong Province,China(2022TZXD006).
文摘Sweet cherry(Prunus avium)represents a significant stone fruit crop in temperate regions worldwide.While molecular breeding has progressed substantially following the initial sweet cherry genome release,existing genome assemblies contain unresolved gaps and comprise consensus chimeric sequences that fail to differentiate haplotype alleles,significantly constraining research on important agronomic trait inheritance.This study presents a phased-resolved telomere-to-telomere reference genome of sweet cherry‘Tieton’.The assembly anchors 653.03 Mb of sequence onto 16 pseudochromosomes representing two haplotypes,with 67,012 coding genes identified(33,777 in hapA and 33,235 in hapB).The genome demonstrates superior quality metrics,including a consensus accuracy exceeding QV44,contig N50 above 17.94 Mb,Benchmarking Universal Single-Copy Orthologs completeness of 98.7%,and a long terminal repeat(LTR)assembly index exceeding 20.This genome provides phased and annotated chromosome pairs,offering a comprehensive view of sweet cherry’s diploid genome organization.Utilizing this reference genome,we identified a large fragment deletion associated with yellow-skinned fruit in sweet cherry‘13-33’.This resource will significantly enhance breeding efforts and genetic research in sweet cherries.
文摘Conversation A(in the break room)Sara:Do I see a picture of a pie?Greg:Youdo.I'm looking at achart of people'sfavoritefall sweettreats.Sara:Yummy!Applepie ismyfavorite.Greg:It'salsothefavoriteof 30percentofpeople.This chartsays that.Sara:Well,apple pie is great.
文摘Located in the heart of southern China’s Jiangnan region,Wuxi is a famous city rich in history and culture during the Spring Festival,showing a unique charm.One of the most interesting features of Wuxi’s New Year celebrations lies in its sweet taste.Unlike many other parts of China where spicy(辛辣)or savory delicacies dominate the feast,Wuxi boasts a tradition of sweet foods during the Spring Festival.
基金Basic Research Program of Jiangsu(Grant No.BK20230112)Earmarked Fund for CARS-10-Sweetpotato+3 种基金Key Research and Development Project of Jiangsu Province(Grant No.BE2019378)Basic Scientific Research Project of Jiangsu Academy of Agricultural Sciences(Grant No.ZX(18)7003)High-level Scientific Research Foundation for the Introduction of Talent of Jinling Institute of Technology(Grant No.jit-b-201914)Jiangsu College Students Innovation and Entrepreneurship Provincial Key Innovation Training Program(Grant No.202213573031Z).
文摘Sweet potato varieties exhibited distinct feedback mechanisms in response to continuous cropping obstacles(CCO).This study evaluated the tolerance to CCO(TCCO)among three types(fresh,purple,and starch),each comprising five varieties,cultivated in a 16-year CCO plot(CCp)and adjacent non-continuous cropping plots(NCCp)in China.Yield,resistance coefficient(k_(Y),yield ratio between CCp and NCCp),and nitrogen(N),phosphorus(P),and potassium(K)contents and relative accumulation(k_(N),k_(P),k_(K),similar to k_(Y))in chunks or stem vines,were analyzed.Significant differences(p<0.05)in yield and nutrient contents were observed among all varieties and types.Four varieties(purple:Xu A1-144,Xu D9-123,and starch:Shang 19,Zhe 13)exhibited k_(Y)>1.0,indicating higher yields under CCp.Nutrient imbalance-particularly enhanced N uptake,was associated with CCO susceptibility.Fresh and purple chunks preferentially accumulated N,P and K,respectively,while starch varieties plants strongly absorbed more K.Under NCCp,chunks nutrient levels were correlated with multiple elements in stem vines.Under CCp,each chunk nutrient was primarily affected only by its homologous elements in stem vines.Notably,stem vine k_(K) positively correlated with yield under CCO(r=0.34,p<0.05),and stem vines k_(N) significantly correlated with both chunks k_(N) and stem vines k_(K)(p<0.05).Starch sweet potatoes demonstrated the most balanced NPK absorption for TCCO,with yield and nutrient absorption advantages.TCCO was closely linked to efficient and coordinated N-K absorption,regulated by genetic traits and soil nutrient status.Imbalanced NPK ratios and hindered K absorption played a central role in CCO.Strategies focusing on K management and breeding varieties with inter-organ nutrient coordination abilities could enhance the stress resistance of sweet potato production systems.These findings provide genetic resources and insight into the mechanism of CCO tolerance in sweet potato.
