Increasing access to locally produced, safe, nutritious and affordable complementary foods is essential to combat micronutrient deficiencies in young children in low- and middle-income countries. Two formulations of s...Increasing access to locally produced, safe, nutritious and affordable complementary foods is essential to combat micronutrient deficiencies in young children in low- and middle-income countries. Two formulations of sweets based on jujube, baobab fruit powder and sweet potato puree were produced, and their nutritional values and sensory properties such as taste, smell, color and acceptability were assessed. The formulation containing the most jujube powder, baobab fruit and sweet potato puree gave the best nutritional value. The sweets are rich in calcium (259.80 mg/100 g), magnesium (67.98 mg/100 g), potassium (782.04 mg/100 g), vitamin C (32.37 mg/100 g), iron (6.45 g mg/100 g) and zinc (0.97 mg/100 g). Sensory tests on the two formulations showed acceptability scores ranging from 81.26% to 84.58%, well above the minimum acceptable threshold. Candies with jujube, baobab fruit powder and sweet potato puree could be an alternative for preventing micronutrient deficiencies.展开更多
SWEET(sugars will eventually be exported transporters)蛋白是近年来鉴定的新型糖转运蛋白,在植物生长发育和果实品质形成过程中有重要作用。为探究金柑SWEET基因家族特点及其在果实发育过程中的表达,从金柑基因组数据库中鉴定出17个...SWEET(sugars will eventually be exported transporters)蛋白是近年来鉴定的新型糖转运蛋白,在植物生长发育和果实品质形成过程中有重要作用。为探究金柑SWEET基因家族特点及其在果实发育过程中的表达,从金柑基因组数据库中鉴定出17个SWEET成员,生物信息学分析表明金柑SWEET基因家族多数成员具有典型的7次跨膜螺旋结构,属于疏水碱性蛋白,在基序和结构域上相对保守。进化分析表明金柑SWEET成员可以分为4类。蛋白质互作网络分析发现FhSWEET16b是重要的核心家族成员。基因表达分析发现FhSWEET1、FhSWEET2b、FhSWEET15和FhSWEET16a在果实发育过程中表达量较高,可能在金柑果实糖分积累中起关键作用。本研究结果为进一步分析柑橘SWEET基因调控果实糖积累的分子机制提供基础。展开更多
Gas-bearing shales have become a major source of future natural gas production worldwide.It has become increasingly urgent to develop a reliable prediction model and corresponding workflow for identifying shale gas sw...Gas-bearing shales have become a major source of future natural gas production worldwide.It has become increasingly urgent to develop a reliable prediction model and corresponding workflow for identifying shale gas sweet spots.The formation of gas-bearing shales is closely linked to relative sealevel changes,providing an important approach to predicting sweet spots in the Wufeng-Longmaxi shale in the southern Sichuan Basin,China.Three types of marine shale gas sweet spots are identified in the shale based on their formation stages combined with relative sea-level changes:early,middle,and late transgression types.This study develops a prediction model and workflow for identifying shale gas sweet spots by analyzing relative sea-level changes and facies sequences.Predicting shale gas sweet spots in an explored block using this model and workflow can provide a valuable guide for well design and hydraulic fracturing,significantly enhancing the efficiency of shale gas exploration and development.Notably,the new prediction model and workflow can be utilized for the rapid evaluation of the potential for shale gas development in new shale gas blocks or those with low exploratory maturity.展开更多
Sweet potato is one of the most important crops in Côte d’Ivoire. However, some constraints related to climate change limit its production. This study evaluated the agronomic and technological performances of ei...Sweet potato is one of the most important crops in Côte d’Ivoire. However, some constraints related to climate change limit its production. This study evaluated the agronomic and technological performances of eight varieties of orange-fleshed of sweet potato (Ipomoea batatas (L.) to identify the most promising ones. The results show three groups of varieties with different agronomic performances. Group 1 composed of Irene and Vita is characterized by low production of small and large tuberous roots, low number of productive plants and low yield. Group 2 consisting mainly of Kabode is characterized by high production and weight of large tuberous roots as well as high yield. Group 3, composed of Tacha-2 Ininda, Ejumula Gloria, TIB-440060, CIP-199062-1, Bela bela is characterized by high production of small and large tuberous roots, high number of productive plants and average yield. The TIB-440060 variety has a recovery rate of 100%. The Irene, Kabode, Vita, Kabode varieties were resistant to viral and fungal diseases. In terms of technological quality, the TIB-440060 and CIP-199062-1 varieties have the best organoleptic qualities. Given the agronomic performances of the Kabode, TIB-440060 and CIP-199062-1 varieties, could be selected as the elites varieties and distributed to producers.展开更多
Conversation A(in the break room)Sara:Do I see a picture of a pie?Greg:Youdo.I'm looking at achart of people'sfavoritefall sweettreats.Sara:Yummy!Applepie ismyfavorite.Greg:It'salsothefavoriteof 30percento...Conversation A(in the break room)Sara:Do I see a picture of a pie?Greg:Youdo.I'm looking at achart of people'sfavoritefall sweettreats.Sara:Yummy!Applepie ismyfavorite.Greg:It'salsothefavoriteof 30percentofpeople.This chartsays that.Sara:Well,apple pie is great.展开更多
Located in the heart of southern China’s Jiangnan region,Wuxi is a famous city rich in history and culture during the Spring Festival,showing a unique charm.One of the most interesting features of Wuxi’s New Year ce...Located in the heart of southern China’s Jiangnan region,Wuxi is a famous city rich in history and culture during the Spring Festival,showing a unique charm.One of the most interesting features of Wuxi’s New Year celebrations lies in its sweet taste.Unlike many other parts of China where spicy(辛辣)or savory delicacies dominate the feast,Wuxi boasts a tradition of sweet foods during the Spring Festival.展开更多
Sweet综合征(sweet syndrome,SS)又称急性发热性嗜中性皮病[1-2],发病机制不明,由Robert Douglas Sweet于1964年首次提出,是一种以发热合并急性发作的皮肤触痛性红斑病变为主要表现的少见特发性疾病。其好发于女性,发病年龄以30~60岁居...Sweet综合征(sweet syndrome,SS)又称急性发热性嗜中性皮病[1-2],发病机制不明,由Robert Douglas Sweet于1964年首次提出,是一种以发热合并急性发作的皮肤触痛性红斑病变为主要表现的少见特发性疾病。其好发于女性,发病年龄以30~60岁居多,男女发病率为1∶4[1-2]。骨髓增生异常综合征/骨髓增殖性肿瘤(MDS/MPN)是一类骨髓增殖成分和无效造血导致血细胞减少共存的混合性髓系肿瘤[3],发病率低,合并Sweet综合征常预示着疾病预后不良。本研究报道1例以Sweet综合征起病的MDS/MPN患者并进行文献复习,现报道如下。展开更多
Sweet potatoes are significant cash crops,however,their yield and quality are greatly compromised by viral diseases.In this study,the complete genomic sequences of two Sweet Potato Virus 2(SPV2)isolates from infected ...Sweet potatoes are significant cash crops,however,their yield and quality are greatly compromised by viral diseases.In this study,the complete genomic sequences of two Sweet Potato Virus 2(SPV2)isolates from infected sweet potato leaves in the Shandong(designated as SPV2-SDYT,GenBank No.PQ855660.1)and Jiangsu(designated as SPV2-JSXZ,GenBank No.PQ855661.1)provinces in China were obtained using 5′RACE and RT-PCR amplification.Consistency,phylogeny,codon usage bias,recombination,and selection pressure analyses were conducted using the SPV2-SDYT and SPV2-JSXZ genome sequences.The complete genome sequences of SPV2-SDYT and SPV2-JSXZ were 10561 nucleotides(nt)in length,with respective nucleotide and amino acid identities of 99.25%and 99.12%,respectively.Both isolates were closely related to the SPV2 isolate from China(SPV2-LN).In both SPV2-SDYT and SPV2-JSXZ,the identity of the P1 protein was the highest,whereas that of the P3 protein was the lowest.There were 26 codons with relatively synonymous codon usage(RSCU)values greater than 1 in SPV2-SDYT and 27 codons with RSCU values greater than 1 in SPV2-JSXZ.High-frequency codons in their genomes were predominantly found to end with A/U.Recombination analysis revealed no major recombination sites in either SPV2-SDYT or SPV2-JSXZ.Further selection pressure analysis showed that the non-synonymous substitution rate/synonymous substitution rate(dN/dS)value of all 10 SPV2 proteins was less than 1.This is the first report on the evolutionary relationships of the 17 known SPV2 isolates.Our findings lay the molecular groundwork for preventing and controlling SPV2 infection in root-tuber crops.These findings also contribute to our understanding of the spread and evolution of SPV2,its pathogenic mechanisms,and the development of antiviral strategies against it.展开更多
Vascular wilt caused by Fusarium oxysporum f.sp.batatas(Fob)is a devastating disease threatening global sweet potato production.To elucidate Fob’s pathogenicitymechanisms and informeffective control strategies,we gen...Vascular wilt caused by Fusarium oxysporum f.sp.batatas(Fob)is a devastating disease threatening global sweet potato production.To elucidate Fob’s pathogenicitymechanisms and informeffective control strategies,we generated a green fluorescent protein(GFP)-tagged Fob strain to track infection dynamics in sweet potato susceptible cultivar Xinzhonghua and resistant cultivar Xiangshu75-55,respectively.Through cytological observation,we found in the susceptible Xinzhonghua,Fob predominantly colonized stem villi,injured root growth points,and directly invaded vascular bundles through stemwounds.Spore germination peaked at 2-3 h post-inoculation(hpi),followed by cyclical mycelial expansion and sporulation within vascular tissues with sustaining infection.In contrast,the resistant Xiangshu75-55 exhibited strong suppression of Fob:spores rarely germinated in vascular bundles or on trichomes by 3 hpi,and mature hyphae were absent in stems at 24 hpi.Quantitative reverse transcription PCR(qRT-PCR)confirmed significantly higher Fob biomass in Xinzhonghua than in Xiangshu75-55 by 16 hpi.Additionally,transcriptional profiling revealed distinct pathogen-host interactions during the compatible and incompatible reactions.In Xinzhonghua,Fob virulence genes FobPGX1,FobICL1,FobCTF2,FobFUB5 and FobFUB6 were upregulated within 16 hpi.Conversely,host defense genes IbMAPKK9,IbWRKY61,IbWRKY75,IbSWEET10,IbBBX24 and IbPIF4 were activated in Xiangshu75-55 during the same period.This study provides spatiotemporal cytological and molecular insights into Fob pathogenicity and host resistance,offering a foundation for early disease detection and improved Fusarium wilt management in sweet potato.展开更多
【目的】糖外排转运蛋白(sugars will eventually be exported transporters,SWEETs)在植物生长发育过程中发挥重要作用,解析SWEETs基因在枸杞果实发育过程中对糖积累作用,为进一步揭示SWEETs基因在枸杞果实发育过程中的作用提供参考。...【目的】糖外排转运蛋白(sugars will eventually be exported transporters,SWEETs)在植物生长发育过程中发挥重要作用,解析SWEETs基因在枸杞果实发育过程中对糖积累作用,为进一步揭示SWEETs基因在枸杞果实发育过程中的作用提供参考。【方法】用生物信息学方法对枸杞SWEET基因(LbaSWEETs)进行全基因组鉴定,并用已发表的转录数据分析LbaSWEETs在果实发育时期的基因表达情况。【结果】枸杞SWEET基因家族共有37个成员,随机分布于10条染色体上,分别编码152~621个氨基酸,蛋白质分子质量为16.87~69.97 kD,等电点为4.96~9.86。亚细胞定位预测位于叶绿体或质膜,大多数含有7个跨膜螺旋。系统进化分析发现,37个LbaSWEETs蛋白可分为4个亚群,每个亚群的基因结构和保守基序组成相似。启动子元件分析表明:Lba-SWEETs基因启动子富含大量激素响应、逆境胁迫和生长发育响应元件。转录组数据和qRT-PCR分析表明:LbaSWEET9和LbaSWEET29基因表达量随果实成熟呈现显著增加。相关性分析结果表明,LbaSWEET9和LbaSWEET29基因表达量与果糖含量呈显著正相关。【结论】LbaSWEET9和LbaSWEET29基因是果糖积累的关键基因。展开更多
SWEET(sugars will eventually be exported transporter)是植物中一类重要的糖转运蛋白,通过调控蔗糖、果糖、葡萄糖、半乳糖等糖类物质的跨膜运输,介导植物根、茎、叶、花、果实、种子等器官中糖类物质的转运、积累和贮存,以参与植物...SWEET(sugars will eventually be exported transporter)是植物中一类重要的糖转运蛋白,通过调控蔗糖、果糖、葡萄糖、半乳糖等糖类物质的跨膜运输,介导植物根、茎、叶、花、果实、种子等器官中糖类物质的转运、积累和贮存,以参与植物各组织的生长发育和逆境胁迫的响应等生理过程。近年来,随着植物中SWEET蛋白功能的明晰,其上游调控机制的研究逐渐增加,其参与的调控网络也被逐步解析。本文总结了植物中SWEET蛋白的主要功能和近几年来其上游调控因子的研究进展,并对SWEET基因家族的研究提出了展望,旨在为进一步揭示SWEET糖转运蛋白的作用机制及其参与的分子调控网络提供参考。展开更多
文摘Increasing access to locally produced, safe, nutritious and affordable complementary foods is essential to combat micronutrient deficiencies in young children in low- and middle-income countries. Two formulations of sweets based on jujube, baobab fruit powder and sweet potato puree were produced, and their nutritional values and sensory properties such as taste, smell, color and acceptability were assessed. The formulation containing the most jujube powder, baobab fruit and sweet potato puree gave the best nutritional value. The sweets are rich in calcium (259.80 mg/100 g), magnesium (67.98 mg/100 g), potassium (782.04 mg/100 g), vitamin C (32.37 mg/100 g), iron (6.45 g mg/100 g) and zinc (0.97 mg/100 g). Sensory tests on the two formulations showed acceptability scores ranging from 81.26% to 84.58%, well above the minimum acceptable threshold. Candies with jujube, baobab fruit powder and sweet potato puree could be an alternative for preventing micronutrient deficiencies.
文摘SWEET(sugars will eventually be exported transporters)蛋白是近年来鉴定的新型糖转运蛋白,在植物生长发育和果实品质形成过程中有重要作用。为探究金柑SWEET基因家族特点及其在果实发育过程中的表达,从金柑基因组数据库中鉴定出17个SWEET成员,生物信息学分析表明金柑SWEET基因家族多数成员具有典型的7次跨膜螺旋结构,属于疏水碱性蛋白,在基序和结构域上相对保守。进化分析表明金柑SWEET成员可以分为4类。蛋白质互作网络分析发现FhSWEET16b是重要的核心家族成员。基因表达分析发现FhSWEET1、FhSWEET2b、FhSWEET15和FhSWEET16a在果实发育过程中表达量较高,可能在金柑果实糖分积累中起关键作用。本研究结果为进一步分析柑橘SWEET基因调控果实糖积累的分子机制提供基础。
文摘Gas-bearing shales have become a major source of future natural gas production worldwide.It has become increasingly urgent to develop a reliable prediction model and corresponding workflow for identifying shale gas sweet spots.The formation of gas-bearing shales is closely linked to relative sealevel changes,providing an important approach to predicting sweet spots in the Wufeng-Longmaxi shale in the southern Sichuan Basin,China.Three types of marine shale gas sweet spots are identified in the shale based on their formation stages combined with relative sea-level changes:early,middle,and late transgression types.This study develops a prediction model and workflow for identifying shale gas sweet spots by analyzing relative sea-level changes and facies sequences.Predicting shale gas sweet spots in an explored block using this model and workflow can provide a valuable guide for well design and hydraulic fracturing,significantly enhancing the efficiency of shale gas exploration and development.Notably,the new prediction model and workflow can be utilized for the rapid evaluation of the potential for shale gas development in new shale gas blocks or those with low exploratory maturity.
文摘Sweet potato is one of the most important crops in Côte d’Ivoire. However, some constraints related to climate change limit its production. This study evaluated the agronomic and technological performances of eight varieties of orange-fleshed of sweet potato (Ipomoea batatas (L.) to identify the most promising ones. The results show three groups of varieties with different agronomic performances. Group 1 composed of Irene and Vita is characterized by low production of small and large tuberous roots, low number of productive plants and low yield. Group 2 consisting mainly of Kabode is characterized by high production and weight of large tuberous roots as well as high yield. Group 3, composed of Tacha-2 Ininda, Ejumula Gloria, TIB-440060, CIP-199062-1, Bela bela is characterized by high production of small and large tuberous roots, high number of productive plants and average yield. The TIB-440060 variety has a recovery rate of 100%. The Irene, Kabode, Vita, Kabode varieties were resistant to viral and fungal diseases. In terms of technological quality, the TIB-440060 and CIP-199062-1 varieties have the best organoleptic qualities. Given the agronomic performances of the Kabode, TIB-440060 and CIP-199062-1 varieties, could be selected as the elites varieties and distributed to producers.
文摘Conversation A(in the break room)Sara:Do I see a picture of a pie?Greg:Youdo.I'm looking at achart of people'sfavoritefall sweettreats.Sara:Yummy!Applepie ismyfavorite.Greg:It'salsothefavoriteof 30percentofpeople.This chartsays that.Sara:Well,apple pie is great.
文摘Located in the heart of southern China’s Jiangnan region,Wuxi is a famous city rich in history and culture during the Spring Festival,showing a unique charm.One of the most interesting features of Wuxi’s New Year celebrations lies in its sweet taste.Unlike many other parts of China where spicy(辛辣)or savory delicacies dominate the feast,Wuxi boasts a tradition of sweet foods during the Spring Festival.
文摘Sweet综合征(sweet syndrome,SS)又称急性发热性嗜中性皮病[1-2],发病机制不明,由Robert Douglas Sweet于1964年首次提出,是一种以发热合并急性发作的皮肤触痛性红斑病变为主要表现的少见特发性疾病。其好发于女性,发病年龄以30~60岁居多,男女发病率为1∶4[1-2]。骨髓增生异常综合征/骨髓增殖性肿瘤(MDS/MPN)是一类骨髓增殖成分和无效造血导致血细胞减少共存的混合性髓系肿瘤[3],发病率低,合并Sweet综合征常预示着疾病预后不良。本研究报道1例以Sweet综合征起病的MDS/MPN患者并进行文献复习,现报道如下。
基金Funding Statement:This work was funded by the National Natural Science Foundation of China(32100132)Shandong Province Natural Sciences Foundation of China(ZR2021QC008)+1 种基金Youth Innovation Team Program'in College of Shandong Province of China(2022KJ119)supported by Young Talent of Lifting Engineering for Science and Technology in Shandong,China(SDAST2024QT085).
文摘Sweet potatoes are significant cash crops,however,their yield and quality are greatly compromised by viral diseases.In this study,the complete genomic sequences of two Sweet Potato Virus 2(SPV2)isolates from infected sweet potato leaves in the Shandong(designated as SPV2-SDYT,GenBank No.PQ855660.1)and Jiangsu(designated as SPV2-JSXZ,GenBank No.PQ855661.1)provinces in China were obtained using 5′RACE and RT-PCR amplification.Consistency,phylogeny,codon usage bias,recombination,and selection pressure analyses were conducted using the SPV2-SDYT and SPV2-JSXZ genome sequences.The complete genome sequences of SPV2-SDYT and SPV2-JSXZ were 10561 nucleotides(nt)in length,with respective nucleotide and amino acid identities of 99.25%and 99.12%,respectively.Both isolates were closely related to the SPV2 isolate from China(SPV2-LN).In both SPV2-SDYT and SPV2-JSXZ,the identity of the P1 protein was the highest,whereas that of the P3 protein was the lowest.There were 26 codons with relatively synonymous codon usage(RSCU)values greater than 1 in SPV2-SDYT and 27 codons with RSCU values greater than 1 in SPV2-JSXZ.High-frequency codons in their genomes were predominantly found to end with A/U.Recombination analysis revealed no major recombination sites in either SPV2-SDYT or SPV2-JSXZ.Further selection pressure analysis showed that the non-synonymous substitution rate/synonymous substitution rate(dN/dS)value of all 10 SPV2 proteins was less than 1.This is the first report on the evolutionary relationships of the 17 known SPV2 isolates.Our findings lay the molecular groundwork for preventing and controlling SPV2 infection in root-tuber crops.These findings also contribute to our understanding of the spread and evolution of SPV2,its pathogenic mechanisms,and the development of antiviral strategies against it.
基金supported by the following grants,Earmarked fund for CARS-10-Sweet potato,High-quality development of agriculture“5511”collaborative innovation project(XTCXGC2021005)Natural Science Foundation of Fujian province(2021J01495)+1 种基金Basic Scientific Research Special Project for Fujian Provincial Public Research Institutes(2021R1031008)Science and Technology Innovation Team of Fujian Academy of Agricultural Sciences(CXTD2021012-1).
文摘Vascular wilt caused by Fusarium oxysporum f.sp.batatas(Fob)is a devastating disease threatening global sweet potato production.To elucidate Fob’s pathogenicitymechanisms and informeffective control strategies,we generated a green fluorescent protein(GFP)-tagged Fob strain to track infection dynamics in sweet potato susceptible cultivar Xinzhonghua and resistant cultivar Xiangshu75-55,respectively.Through cytological observation,we found in the susceptible Xinzhonghua,Fob predominantly colonized stem villi,injured root growth points,and directly invaded vascular bundles through stemwounds.Spore germination peaked at 2-3 h post-inoculation(hpi),followed by cyclical mycelial expansion and sporulation within vascular tissues with sustaining infection.In contrast,the resistant Xiangshu75-55 exhibited strong suppression of Fob:spores rarely germinated in vascular bundles or on trichomes by 3 hpi,and mature hyphae were absent in stems at 24 hpi.Quantitative reverse transcription PCR(qRT-PCR)confirmed significantly higher Fob biomass in Xinzhonghua than in Xiangshu75-55 by 16 hpi.Additionally,transcriptional profiling revealed distinct pathogen-host interactions during the compatible and incompatible reactions.In Xinzhonghua,Fob virulence genes FobPGX1,FobICL1,FobCTF2,FobFUB5 and FobFUB6 were upregulated within 16 hpi.Conversely,host defense genes IbMAPKK9,IbWRKY61,IbWRKY75,IbSWEET10,IbBBX24 and IbPIF4 were activated in Xiangshu75-55 during the same period.This study provides spatiotemporal cytological and molecular insights into Fob pathogenicity and host resistance,offering a foundation for early disease detection and improved Fusarium wilt management in sweet potato.
文摘【目的】糖外排转运蛋白(sugars will eventually be exported transporters,SWEETs)在植物生长发育过程中发挥重要作用,解析SWEETs基因在枸杞果实发育过程中对糖积累作用,为进一步揭示SWEETs基因在枸杞果实发育过程中的作用提供参考。【方法】用生物信息学方法对枸杞SWEET基因(LbaSWEETs)进行全基因组鉴定,并用已发表的转录数据分析LbaSWEETs在果实发育时期的基因表达情况。【结果】枸杞SWEET基因家族共有37个成员,随机分布于10条染色体上,分别编码152~621个氨基酸,蛋白质分子质量为16.87~69.97 kD,等电点为4.96~9.86。亚细胞定位预测位于叶绿体或质膜,大多数含有7个跨膜螺旋。系统进化分析发现,37个LbaSWEETs蛋白可分为4个亚群,每个亚群的基因结构和保守基序组成相似。启动子元件分析表明:Lba-SWEETs基因启动子富含大量激素响应、逆境胁迫和生长发育响应元件。转录组数据和qRT-PCR分析表明:LbaSWEET9和LbaSWEET29基因表达量随果实成熟呈现显著增加。相关性分析结果表明,LbaSWEET9和LbaSWEET29基因表达量与果糖含量呈显著正相关。【结论】LbaSWEET9和LbaSWEET29基因是果糖积累的关键基因。
文摘SWEET(sugars will eventually be exported transporter)是植物中一类重要的糖转运蛋白,通过调控蔗糖、果糖、葡萄糖、半乳糖等糖类物质的跨膜运输,介导植物根、茎、叶、花、果实、种子等器官中糖类物质的转运、积累和贮存,以参与植物各组织的生长发育和逆境胁迫的响应等生理过程。近年来,随着植物中SWEET蛋白功能的明晰,其上游调控机制的研究逐渐增加,其参与的调控网络也被逐步解析。本文总结了植物中SWEET蛋白的主要功能和近几年来其上游调控因子的研究进展,并对SWEET基因家族的研究提出了展望,旨在为进一步揭示SWEET糖转运蛋白的作用机制及其参与的分子调控网络提供参考。
